CN105137094A - Immuno-electrochemical paper chip electrode - Google Patents
Immuno-electrochemical paper chip electrode Download PDFInfo
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- CN105137094A CN105137094A CN201510616490.2A CN201510616490A CN105137094A CN 105137094 A CN105137094 A CN 105137094A CN 201510616490 A CN201510616490 A CN 201510616490A CN 105137094 A CN105137094 A CN 105137094A
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- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/76—Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors
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Abstract
The invention discloses an immuno-electrochemical paper chip. The immuno-electrochemical paper chip comprises a bottom plate, a reference electrode layer and a working electrode layer, wherein the working electrode layer comprises a plurality of central shunting regions, a plurality of micro-channels, a detection region and a plurality of working electrodes; each micro-channel is used for leading a sample to be detected from the detection region to the corresponding central shunting region; each working electrode is positioned in the corresponding central shunting region; different antibodies are fixed onto the surfaces of the working electrodes and are used for performing multi-parameter joint detection on FSH, LH and E2 respectively. The immuno-electrochemical paper chip is in a multi-layer combined design, functions of sampling, separating, immuno-detection and the like are achieved on a single paper chip, the operation is simple, and the detection is rapid; meanwhile, an electrochemical immuno-detection method is directly adopted, secondary antibodies do not need to be added, needed reagents are few, and manual operation errors can be reduced.
Description
Technical field
The present invention relates to detection paper and paper chip technical field, relate more specifically to a kind of immune galvanochemistry paper chip electrode detected fast for sex hormone multiparameter.
Background technology
The function of assessment ovary understands the key of female reproductive health, and the method detecting Ovary reserve that adopts realizes more clinically.Ovary reserve monitoring is at disease of ovary diagnosis (as premature ovarian failure, sex premature, paramenia, polycystic ovary syndrome and climacteric etc.), result for the treatment of assessment (as ovary responsiveness assessment during ovulation treatment), standby pregnant guidance and female pathology health etc. have very important significance.Assess the clinical indices such as Ovary reserve Primary Reference age, FSH (FSH), FSH/LH (lutropin), basic estradiol (E2) clinically at present.Wherein FSH, FSH/LH, E2 are the most frequently used Testing index, and these three joint-detection can meet the primary demand that clinician and scientific research personnel monitor Ovary reserve substantially.
In the evaluation to ovarian reserve power, by E2 level and age and FSH, and FSH/LH horizontal integration is got up, and better can evaluate the reserve capabillity of ovary.As basic FSH level is normal, but E2 level raises, then ovulation induction mortality increases, and pregnancy rate declines.Therefore survey blood E2 and FSH level than single mensuration FSH or E2 predictablity rate higher.E2 level has more valuable supplementary meaning to the reaction and final result of estimating the IVF cycle.Because ovarian function is a kind of cyclical variation, the growth quality and quantity of Hormone traits and each menstrual cycle ovarian follicle is closely related, therefore dynamic monitoring LH, FSH and E2 change for understanding ovarian function so that follicular development all most important.Existing detection means is for the somatic damage needing the patient of dynamic monitoring ovarian function not only to there is financial burden, the loss of time and venous blood collection repeatedly, meanwhile, the outpatient service that very long consultation process is also hospital with the wait of the result of laboratory test reaching 6-12 hour brings huge load.The World Health Organization (WHO) propose desirable diagnosis detecting method should have low price, test responsive, special, be easy to use, detect quick, without the need to instrument, be easy to the feature such as shared, therefore explore a kind of easy and simple to handle, immediately can be obtained the method for the quantitative data of FSH, LH and E2 by the detection of micro blood (1-2 bleeds), for clinical ovarian function dynamic monitoring, instruct the tailored diagnostics of standby pregnant and even ovarian function disease and treatment to have important directive significance.
The sex hormone such as LH, FSH and E2 content in blood of human body is extremely low, need higher detection sensitivity, current detection mainly adopts immunization method, such as immune colloid gold examination bar and the method such as ELISA, chemiluminescence, electrochemiluminescence based on immune biochemical detector.Wherein colloid gold test strip is based on immune chromatography method, and structure is simple, and easy to operate, price is low, but owing to adopting immune coloration method, be qualitative or half-quantitative detection, sensitivity is not high, cannot meet quantitative detection demand.Immune biochemical detector can realize multiparameter and detect simultaneously, detection sensitivity is high, but sense cycle is long, need sample amount large (adopting venous blood collection 3-4ml), and immunity analysis instrument mostly is large-scale instrument, price is high, is suitable for clinical laboratory or the centralab of hospital, is difficult to the demand meeting family, individual detects fast to LH, FSH and E2 etc.
Micro-fluidic paper chip take filter paper as base material, there is the function of micro-fluidic chip, can complete the functions such as application of sample, filtration, detection on a single die, and structure is simple, with low cost, few by sample amount, can realize the trace of biomolecule, multiparameter joint-detection.The Whitesides seminar of Harvard University in 2007 develops the first paper chip detected for biological sample, has carried out a series of development work afterwards in the method for making of chip, fluid operated principle and detection method etc.Electrochemical immunoassay method combines the advantage of immunoassay and Electrochemical Detection, utilizes immune response to catch determinand, is changed detect testing concentration by electrochemical signals, there is pick-up unit simple, easy and simple to handle, sensitivity advantages of higher, can meet LH, FSH and E2 and detect demand.
But, the paper chip of current employing Direct Electrochemistry detection method mostly is dropping sample, large by sample amount, need in paper chip development process, to solve reduction sample use amount, reduce the key scientific problems that the microstructures such as micro-raceway groove, micro-reaction zone were disturbed, optimized in different parameters detection mutually; Sex hormone in vivo content is extremely low, and Direct Electrochemistry immune detection paper chip is subject to electrode and prepares modification condition and environmental factor interference, needs to solve the problem in science such as the quantitative detection of ultratrace sex hormone, electrode face finish and bio-sensing film preparation.
Summary of the invention
In view of this, one object of the present invention is to provide a kind of immune galvanochemistry paper chip.Another object of the present invention is that providing a kind of adopts above-mentioned immune galvanochemistry paper chip sex hormone to be carried out to the method detected fast.
To achieve these goals, as one aspect of the present invention, the invention provides a kind of immune galvanochemistry paper chip, comprising:
Base plate, is provided with output electrode, is respectively used to working electrode, is electrically connected electrode and reference electrode, described working electrode, to form measure loop to electrode and reference electrode and external detection instrument;
Contrast electrode layer, is provided with electrode and reference electrode; And
Electrode layer, comprise shunting zone, multiple center, multiple micro-raceway groove, detection zone and multiple working electrode, wherein said multiple micro-raceway groove be used for respectively by detected sample from described detection zone water conservancy diversion to the shunting zone, center of correspondence, described multiple working electrode lays respectively at corresponding shunting zone, center, its surface is fixed with different antibodies respectively, for the hormone of catching in described detected sample.
As another aspect of the present invention, present invention also offers a kind of preparation method of working electrode, comprise the following steps:
In graphene oxide, add poly-acetimide PEI, under backflow effect, heat multiple hours, obtain redox graphene;
Thionine molecule is adsorbed onto the surface of described redox graphene by pi-pi accumulation effect;
React the amido on golden nanometer particle and described redox graphene formation compound;
Select carbon electrode as working electrode, described working electrode is immersed in described compound, thus on described working electrode, form gold nano/thionine/graphene nano composite membrane;
The amino effect of required antibody by nm of gold and protein is fixed on described working electrode.
As another aspect of the invention, present invention also offers a kind of preparation method of immune galvanochemistry paper chip, comprise the following steps:
Prepare each ingredient of immune galvanochemistry paper chip described above respectively;
Each ingredient prepared is fitted together.
As an also aspect of the present invention, present invention also offers a kind of sex hormone multiparameter method for quick, adopt immune galvanochemistry paper chip described above to detect detected sample.
Known based on technique scheme, immune galvanochemistry paper chip electrode of the present invention and adopt its detection method to have following beneficial effect: (1) multiparameter test paper of the present invention chip detects demand in the face of household person, professional software is adopted to carry out high precision design, the microstructures such as micro-raceway groove and micro-reaction zone are formed by wax spray printing and exact heat wax melting, to reduce sample use amount, meet trace detection (1-2 bleeds) demand; Because sensor adopts flow control accurately, ensure that liquid can flow to three detection zones uniformly, detect not by interference mutually, achieve multiparameter joint-detection.Employing multiple layer combination designs, and single paper chip realizes the functions such as application of sample, separation, immune detection, simple to operate, detects fast, adopts Direct Electrochemistry immunologic detection method simultaneously, and need not add two and resist, required reagent is few, can reduce manual operation error; By emulating and test the method combined, optimize the flow passage structure of paper chip, ensure that liquid can flow to three detection zones uniformly, detect not by interference mutually;
(2) adopt Graphene, golden nanometer particle to form composite nano materials to modify working electrode, increase the electroactive of electron mediator, improve detection sensitivity; Adopt metal nanoparticle fixed trapped antibody, optimize antibody fixed form, increase biomolecule supported quantity, improve its reactivity;
(3) the invention provides one easy and simple to handle, immediately can be obtained the method for the quantitative data of FSH, LH and E2 by the detection of micro blood (1-2 bleeds), for clinical ovarian function dynamic monitoring, instruct the tailored diagnostics of standby pregnant and even ovarian function disease and treatment to have important directive significance.
Accompanying drawing explanation
Fig. 1 is the STRUCTURE DECOMPOSITION schematic diagram of immune galvanochemistry paper chip of the present invention;
Fig. 2 is the tectum structure schematic diagram of immune galvanochemistry paper chip of the present invention;
Fig. 3 is the layers of two-sided structural representation of immune galvanochemistry paper chip of the present invention;
Fig. 4 is the electrode layer structural representation of immune galvanochemistry paper chip of the present invention;
Fig. 5 is the preparation section process flow diagram of the electrode layer of immune galvanochemistry paper chip of the present invention;
Fig. 6 is the preparation section process flow diagram of the contrast electrode layer of immune galvanochemistry paper chip of the present invention;
Fig. 7 is the preparation section process flow diagram of the base plate of immune galvanochemistry paper chip of the present invention.
Embodiment
For making the object, technical solutions and advantages of the present invention clearly understand, below in conjunction with specific embodiment, and with reference to accompanying drawing, the present invention is described in further detail.
The invention discloses a kind of immune galvanochemistry paper chip, comprising:
Base plate, is provided with output electrode, is respectively used to working electrode, is electrically connected electrode and reference electrode, described working electrode, to form measure loop to electrode and reference electrode and external detection instrument;
Contrast electrode layer, is provided with electrode and reference electrode; And
Electrode layer, comprise shunting zone, multiple center, multiple micro-raceway groove, detection zone and multiple working electrode, wherein said multiple micro-raceway groove be used for respectively by detected sample from described detection zone water conservancy diversion to the shunting zone, center of correspondence, described multiple working electrode lays respectively at corresponding shunting zone, center, its surface is fixed with different antibodies respectively, for the hormone of catching in described detected sample.
It is superimposed that three layers can adopt arbitrary overlay order to carry out as required, as long as the electrode of correspondence can form electric loop, and do not affect normal sample introduction.
Wherein, working electrode can adopt conventional working electrode, and such as carbon electrode, quantity is preferably 3, is respectively used to carry out multiparameter joint-detection to FSH, LH and E2.As preferably, working electrode adopts thionine as electron mediator, modifies gold nano/thionine/graphene nanometer composite on the working electrode (s.As preferably, the detection zone of electrode layer can also being provided with filtering membrane, for the cell separation in detected sample being retained, only allowing filtering membrane described in all the other material permeances to enter described detection zone, thus improve detection sensitivity.
Immunity galvanochemistry paper chip also comprises overlayer, and overlayer covers the upper surface of whole described immune galvanochemistry paper chip, and is hydrophobic structure.Overlayer such as can adopt rigid plastic to prepare.
Next-door neighbour's overlayer can also be provided with adhesive phase, adhesive phase is for being adhesively fixed in this immune galvanochemistry paper chip by overlayer, and adhesive phase is hydrophobic structure, is formed with sample introduction tank over the binder layer, for by detected sample water conservancy diversion through filtering membrane.In said structure, also can not adhesive phase be set, directly overlayer be fixed in this immune galvanochemistry paper chip by hot-forming technique, and form corresponding sample introduction tank on the cover layer.
The invention also discloses a kind of preparation method of working electrode, comprise the following steps:
In graphene oxide, add poly-acetimide PEI, under backflow effect, heat multiple hours, obtain redox graphene;
Thionine molecule is adsorbed onto the surface of redox graphene by pi-pi accumulation effect;
React the amido on golden nanometer particle and redox graphene formation compound;
Select carbon electrode as working electrode, working electrode is immersed in this compound, thus form gold nano/thionine/graphene nano composite membrane on the working electrode (s;
Required antibody is fixing on the working electrode (s by the amino effect of nm of gold and protein.
The preparation method of this working electrode may be used for the working electrode preparing fixing FSH, LH and E2 antibody, thus is respectively used to detect the indexs such as FSH, LH and E2.
The invention also discloses a kind of preparation method of immune galvanochemistry paper chip, comprise the following steps:
Prepare each ingredient of immune galvanochemistry paper chip as above respectively;
Each ingredient prepared is fitted together.
Wherein, immune galvanochemistry paper chip comprises FSH, LH and E2 tri-kinds of working electrodes, and FSH, LH and E2 working electrode adopts working electrode preparation method as above to be prepared.
The invention also discloses a kind of sex hormone multiparameter method for quick, adopt immune galvanochemistry paper chip as above to detect detected sample.
Below in conjunction with accompanying drawing, explanation is described in detail to a preferred embodiment of the present invention.
Paper chip 0 of the present invention as shown in Figure 1, comprises overlayer 1, layers of two-sided 2, filtering membrane 3, electrode layer 4, contrast electrode layer 5 and base plate 6.
Wherein, overlayer 1 selects rigid plastic, is cut into as Fig. 2 structure, and it acts on mainly: (1) and floor combination form the paper chip with certain degree of hardness; (2) it is hydrophobic structure, and after avoiding application of sample, sample is loose overflows; (3) and electrode layer, bonded by layers of two-sided, form micro-raceway groove, by the suction sample introduction of kapillary.
Wherein, layers of two-sided 2 selects conventional medical dual-side adhesive tape, has bonding effect and hydrophobic property, is cut into as shown in Figure 3.It act as: (1) can by each layer bonding by adhesive effect; (2) layers of two-sided is opened a noise, as the micro-raceway groove 2-1 of sample introduction, by capillarity, finger blood is drawn to shunting zone, then flows to detection zone through filter membrane.
Filtering membrane 3 adopts fiber film material, is cut into circle, and effect is trapped on film by cell separation such as the red blood cell in blood of human body liquid, leucocytes, and all the other materials then enter liquid center shunting zone through filtering membrane.
Electrode layer 4 adopts filter paper: (1) adopts sectional drawing machine that filter paper is cut into A4 size; (2) adopt wax spray printer to carry out wax spray to filter paper and print 4-1, as shown in Figure 4, wherein black part is divided into wax spray part 4-2, the blank 4-3 of white portion; (3) adopt accurate numerical control heating instrument to heat printed filter paper, wax is melted and forms hydrophobic region 4-4, formation center, clear area shunting zone 4-5, micro-raceway groove 4-6 and detection zone 4-7; (4) silk-screen mode is adopted at detection zone silk-screen carbon slurry electrode as working electrode 4-8, simultaneously silk-screen line 4-9 and switching electrode 4-10; (5) to working electrode electrode face finish, different antibodies is fixed respectively to catch three kinds of hormones.
The present invention carries out multiparameter joint-detection to FSH, LH and E2, and working electrode has three, is respectively used to detect FSH, LH and E2.For FSH detecting electrode, the present invention adopts Direct Electrochemistry immunization method the method to combine the advantage of immunoassay and Electrochemical Detection, compared with indirect electrochemical immunization method, resists, operate easier without the need to two of marker enzyme.The present invention adopts thionine as electron mediator, modifies gold nano/thionine/graphene nanometer composite on the working electrode (s.Because poly-thionine have good electrochemical reversibility, stability and fast electron transmission ability be a kind of good electron mediator, and containing two-NH
2base, can adsorb electronegative nanogold particle by covalent bond and electrostatic interaction, thus forms nm of gold individual layer.And Graphene possesses the unique physico-chemical character such as monolithic, high conductance, bigger serface, mithridatism and good electronics biography degree dynamics, be widely used in electrochemical sensing and bio-sensing.
Working electrode preparation process as shown in Figure 5, comprise: (1) prepares redox graphene (rGO), concrete steps comprise: in graphene oxide (GO), add poly-acetimide (PEI), under backflow effect, 135 DEG C are heated 3 hours, after cleaning and centrifuging, obtain redox graphene (rGO).(2) thionine molecule (Thi) is adsorbed onto the surface of redox graphene by pi-pi accumulation effect, and (3) golden nanometer particle is fixed by reacting with the amido on redox graphene thus formed compound.(4) working electrode is carbon electrode, after surface treatment, then becomes gold nano/thionine/graphene nano composite membrane on the working electrode (s.(5) add FSH antibody, by the amino effect of nm of gold and protein, antibody is fixed on two and does on electrode.(6) for reducing non-specific adsorption, finally unnecessary avtive spot is closed with bovine serum albumin(BSA).
Contrast electrode layer 5 adopts filter paper: (1) adopts sectional drawing machine that filter paper is cut into A4 size, (2) adopt wax spray printer to carry out wax spray to filter paper and print 5-1, as shown in Figure 6, wherein black part is divided into wax spray part 5-2, the blank 5-3 of white portion.(3) adopt accurate numerical control heating instrument to heat printed filter paper, wax is melted and forms hydrophobic region 5-4, formation center, clear area 5-5.(4) silk-screen mode is adopted at center silk-screen to electrode (carbon electrode) 5-6 and reference electrode (Ag/Agcl) 5-7, simultaneously silk-screen line 5-8 and switching electrode 5-9.
Base plate 6 adopts the double faced adhesive tape of AR company, preparation flow is as Fig. 7: (1) first adopts film-engraving machine on double faced adhesive tape, scribe figure 6-1, (2) model is thrown off, gold electrode structure 6-2 is formed by the mode of sputtering, comprise output electrode 6-3, line 6-4, working electrode bonding pad 6-5, to electrode connecting region 6-6, reference electrode bonding pad 6-7.Wherein, 6-5 tri-gold-plated districts in working electrode bonding pad are respectively at the switching electrode alignment of 3 in electrode layer, and close contact realizes connecting; Electrode connecting region 6-6 and reference electrode bonding pad 6-7 is alignd to electrode 5-4 and reference electrode 5-5 respectively with on contrast electrode layer, and close contact realizes connecting; Working electrode bonding pad 6-5, electrode connecting region 6-6 to be connected with output electrode 6-3 respectively by line with reference electrode bonding pad 6-7; Output electrode directly can be connected with detecting instrument, and base plate is hard material, and output electrode is the gold electrode sound construction of sputtering, and the paper chip of therefore inventing can adopt the mode of grafting to be connected with instrument.(3) base plate is cut into structure as shown in 6-8 in figure, wherein center drilling forms Chu Yang district 6-9, for cleaning.
Overlayer 1, layers of two-sided 2, filtering membrane 3, electrode layer 4, contrast electrode layer 5 and base plate 6 are assembled according to the mode of Fig. 1 by paper chip 0, wherein overlayer 1, layers of two-sided 2 are all 25mm mutually with base plate 6 width, the wide 15mm of electrode layer 4, contrast electrode layer 5 width 20mm, each layer can be sticked together by double faced adhesive tape like this, form enclosed construction.Wherein one of electrode layer 4 and contrast electrode layer 5 screen printed electrode facing to the one side of base plate 6 sputtering electrode, ensure during encapsulation that the switching electrode 4-10 of electrode layer 4 and the electrode layer bonding pad 6-5 of base plate 6 is adjacent to, and two of contrast electrode layer 5 are transferred electrode 5-9 respectively with the contrast electrode bonding pad 6-6 of base plate 6 be adjacent to electrode connecting region 6-7.
Above-described specific embodiment; object of the present invention, technical scheme and beneficial effect are further described; be understood that; the foregoing is only specific embodiments of the invention; be not limited to the present invention; within the spirit and principles in the present invention all, any amendment made, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. an immune galvanochemistry paper chip, comprising:
Base plate, is provided with output electrode, is respectively used to working electrode, is electrically connected electrode and reference electrode, described working electrode, to form measure loop to electrode and reference electrode and external detection instrument;
Contrast electrode layer, is provided with electrode and reference electrode; And
Electrode layer, comprise shunting zone, multiple center, multiple micro-raceway groove, detection zone and multiple working electrode, wherein said multiple micro-raceway groove be used for respectively by detected sample from described detection zone water conservancy diversion to the shunting zone, center of correspondence, described multiple working electrode lays respectively at corresponding shunting zone, center, its surface is fixed with different antibodies respectively, for the hormone of catching in described detected sample.
2. immune galvanochemistry paper chip as claimed in claim 1, wherein said multiple working electrode is 3 working electrodes, is respectively used to carry out multiparameter joint-detection to FSH, LH and E2.
3. immune galvanochemistry paper chip as claimed in claim 1, wherein said multiple working electrode adopts thionine as electron mediator, modifies gold nano/thionine/graphene nanometer composite on the working electrode (s.
4. immune galvanochemistry paper chip as claimed in claim 1, the detection zone of wherein said electrode layer is also provided with filtering membrane, for the cell separation in detected sample being retained, only allows filtering membrane described in all the other material permeances to enter described detection zone.
5. immune galvanochemistry paper chip as claimed in claim 4, wherein said immune galvanochemistry paper chip also comprises overlayer, and described overlayer covers the upper surface of whole described immune galvanochemistry paper chip, and is hydrophobic structure.
6. immune galvanochemistry paper chip as claimed in claim 4, wherein be close to described overlayer and be also provided with adhesive phase, described adhesive phase is for being adhesively secured to described immune galvanochemistry paper chip by described overlayer, and described adhesive phase is hydrophobic structure, described adhesive phase is formed with sample introduction tank, for by detected sample water conservancy diversion through described filtering membrane.
7. a preparation method for working electrode, comprises the following steps:
In graphene oxide, add poly-acetimide PEI, under backflow effect, heat multiple hours, obtain redox graphene;
Thionine molecule is adsorbed onto the surface of described redox graphene by pi-pi accumulation effect;
React the amido on golden nanometer particle and described redox graphene formation compound;
Select carbon electrode as working electrode, described working electrode is immersed in described compound, thus on described working electrode, form gold nano/thionine/graphene nano composite membrane;
The amino effect of required antibody by nm of gold and protein is fixed on described working electrode.
8. a preparation method for immune galvanochemistry paper chip, comprises the following steps:
Prepare each ingredient of the immune galvanochemistry paper chip as described in claim 1 to 6 any one respectively;
Each ingredient prepared is fitted together.
9. the preparation method of immune galvanochemistry paper chip as claimed in claim 8, wherein said immune galvanochemistry paper chip comprises FSH, LH and E2 working electrode, and described FSH, LH and E2 working electrode adopts the preparation method of working electrode as claimed in claim 7 to be prepared.
10. a sex hormone multiparameter method for quick, adopts the immune galvanochemistry paper chip as described in claim 1 to 6 any one to detect detected sample.
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| CN201510616490.2A CN105137094A (en) | 2015-09-24 | 2015-09-24 | Immuno-electrochemical paper chip electrode |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106199013A (en) * | 2016-08-16 | 2016-12-07 | 北京大学第医院 | A kind of low abundance hormone test method and device |
| CN106841591A (en) * | 2017-02-27 | 2017-06-13 | 同昕生物技术（北京）有限公司 | The test card that a kind of capillary force drives |
| CN106996929A (en) * | 2017-03-10 | 2017-08-01 | ***广州总医院 | Integrated electrochemical luminescence papery micro-fluidic chip and preparation method and application |
| CN107656083A (en) * | 2017-09-21 | 2018-02-02 | 中国科学院电子学研究所 | From sample introduction immune detection paper chip and preparation method thereof |
| CN108663419A (en) * | 2018-06-11 | 2018-10-16 | 中国科学院电子学研究所 | Paper chip and preparation method thereof and biomolecule detecting method |
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| CN109061190A (en) * | 2018-08-22 | 2018-12-21 | 江苏集萃微纳自动化***与装备技术研究所有限公司 | The preparation of multichannel biosensor array and immune detection application based on paper chip |
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| CN114324509A (en) * | 2020-09-27 | 2022-04-12 | 南京基蛋生物医药有限公司 | Graphene modified electrode for blood gas biochemical index analysis and manufacturing method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106199013A (en) * | 2016-08-16 | 2016-12-07 | 北京大学第医院 | A kind of low abundance hormone test method and device |
| CN106199013B (en) * | 2016-08-16 | 2018-05-01 | 北京大学第一医院 | A kind of low abundance hormone test method and device |
| CN106841591A (en) * | 2017-02-27 | 2017-06-13 | 同昕生物技术（北京）有限公司 | The test card that a kind of capillary force drives |
| CN106996929A (en) * | 2017-03-10 | 2017-08-01 | ***广州总医院 | Integrated electrochemical luminescence papery micro-fluidic chip and preparation method and application |
| CN107656083A (en) * | 2017-09-21 | 2018-02-02 | 中国科学院电子学研究所 | From sample introduction immune detection paper chip and preparation method thereof |
| CN108663419A (en) * | 2018-06-11 | 2018-10-16 | 中国科学院电子学研究所 | Paper chip and preparation method thereof and biomolecule detecting method |
| CN109060916A (en) * | 2018-07-31 | 2018-12-21 | 山东师范大学 | Electrochemical sensor electrodes nano surface material method of modifying and sensor |
| CN109061190A (en) * | 2018-08-22 | 2018-12-21 | 江苏集萃微纳自动化***与装备技术研究所有限公司 | The preparation of multichannel biosensor array and immune detection application based on paper chip |
| CN109061190B (en) * | 2018-08-22 | 2021-12-07 | 苏州微湃医疗科技有限公司 | Preparation of multi-channel biosensor array based on paper chip and application of multi-channel biosensor array in immunodetection |
| CN110455895A (en) * | 2019-08-20 | 2019-11-15 | 青岛科技大学 | Graphene oxide/houghite electrochemical DNA biosensor and its preparation method and application of thionine functionalization reduction |
| CN114324509A (en) * | 2020-09-27 | 2022-04-12 | 南京基蛋生物医药有限公司 | Graphene modified electrode for blood gas biochemical index analysis and manufacturing method thereof |
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Application publication date: 20151209 |