CN105132531B - A kind of method and its application identified or aid in identification soybean kernel palmitic acid content - Google Patents
A kind of method and its application identified or aid in identification soybean kernel palmitic acid content Download PDFInfo
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Abstract
The invention discloses a kind of method and its application identified or aid in identification soybean kernel palmitic acid content.A kind of method identified or aid in identification soybean kernel palmitic acid content character provided by the invention, comprises the following steps：Detect the genotype based on Map 6064SNP sites in soybean gene group to be measured, if the soybean kernel with low palmitic acid content character that TT is homozygous, soybean kernel to be measured is candidate, if the soybean kernel with high palmitic acid content character that CC is homozygous, soybean kernel to be measured is candidate；The high palmitic acid content refers to that palmitic acid content is more than 12.3%；The low palmitic acid content refers to that palmitic acid content is less than 12.3%.It is demonstrated experimentally that a kind of method for identifying or aiding in identification soybean kernel palmitic acid content character provided by the present invention can be used for identifying or aid in identifying the height of palmitic acid content in soybean varieties to be measured.
Description
Technical field
The present invention relates to biological technical field, more particularly to a kind of identification or auxiliary to identify soybean kernel palmitic acid content
Method and its application.
Background technology
Soybean (Glycine max) is oil plant cereal crops important in the world, and its seed oil is the mankind and Animal nutrition
And the important sources of food processing industry vegetable edible oil.Soybean oil dosage accounts for 31% (Kim and of whole world edible oil
Krishnan, 2004).Soybean seed oil is mainly what is be made up of glycerine and aliphatic acid, and wherein aliphatic acid accounts for the 90% of seed oil
More than, mainly including palmitic acid (16:0) with stearic acid (18:0) two kinds of saturated fatty acids and oleic acid (18:1), linoleic acid (18:
2) with leukotrienes (18:3) three kinds of unrighted acids such as.Composition and its species proportioning of aliphatic acid determine the product of seed oil
Matter.Palmitic acid is saturated fatty acid, non-digestible, and the mankind are excessively edible, can cause fat and angiocardiopathy, is reduced big
Beans palmitic acid content can increase the nutritive value of soybean.
Due to palmitic acid content be by controlled by multiple genes quantitative character and easily it is affected by environment, utilize traditional phenotypic evaluation
The kind of low palmitic acid content is cultivated with breeding method not only to be needed to take a long time, and is difficult to success, can not
Enough meet the development of current high quality soybean breeding.With the exploitation and use of molecular labeling, molecular marker assisted selection
(Molecular marker-assisted selection, MAS), which turns into, can save human and material resources and acceleration breeding process
Effective ways (Cregan et al.1999).The great advantage of molecular marker assisted selection is that need not assess phenotype spy
In the case of sign, by being confirmed whether to identify the plant of low palmitic acid content with target gene.
Be richly stored with gene in Soybean Germplasm, required for being excavated from material base-germ plasm resource of breeding
Excellent resources and gene can effectively facilitate soybean varieties improvement.As the cultivated soybean area of origin, the Soybean Germplasm in China
The abundantest in the world, be now stored in national germplasm resource bank just has more than 30,000 parts, is made a variation on palmitic acid content extensive
(Liu Xingyuan etc., Crop Germplasm Resources, 1998).In order to effectively study and utilize the (Crop Sciences such as China's soybean resource, Qiu Lijuan
Report, 2009) construct and can represent the multifarious Core Germplasms of China's Soybean Germplasm, to contain in deep excavation germ plasm resource
Favorable genes, effectively widen soybean heredity basis and established material foundation.
Association analysis, also known as association mapping (Association Mapping) are one kind with LD (Linkage
Disequlibrium based on) analyzing, directly genotypic variation and phenotypic variation are analyzed, so as to those and character
Related identified for genes comes out the gene positioning method of (Khush et al.2001), it has also become excavates and phenotype correlation molecule
The powerful tool of mark.New molecular marker-SNP because with genome quantity it is more, it is widely distributed, can high flux
The advantages that detection and be widely used in recent years identification controlled by multiple genes complex character.With being on the increase for SNP marker quantity,
High-throughout SNP partings platform releases one after another, including Goladen Gate and BeadArray (Illumina), GenomeLab
SNP stream (Beckman) and MegAllele (Affymetrix) etc., wherein Illumina companies BeadArray chips
Identification technology have the advantages that efficiently, high flux, cost is low, accuracy is good, be adapted to number of sites from tens to thousands of medium logical
Measure Genotyping research, experiment success rate>99%, it is the solution of preferably middle high flux Genotyping detection, at present
It is widely used in the SNP partings of the species such as the mankind, arabidopsis, rice.
The content of the invention
The purpose of the present invention is identification or auxiliary identifies soybean kernel palmitic acid content character, and/or, screening or auxiliary sieve
Soybean kernel of the choosing with different palmitic acid contents.
To contain in order to solve the above technical problems, present invention firstly provides one kind identification or auxiliary identification soybean kernel palmitic acid
The method for measuring character, it may include following steps：The genotype based on Map-6064SNP sites in soybean gene group to be measured is detected,
It is pure if CC if the soybean kernel with low palmitic acid content character that TT is homozygous, soybean kernel to be measured is candidate
Mould assembly, the soybean kernel with high palmitic acid content character that soybean kernel to be measured is candidate.
In order to solve the above technical problems, present invention also offers one kind screening or assisting sifting to have different palmitic acid contents
Soybean kernel method, it may include following steps：Detect the gene based on Map-6064SNP sites in soybean gene group to be measured
Type, if the soybean kernel with low palmitic acid content character that TT is homozygous, soybean kernel to be measured is candidate, if CC
Soybean kernel homozygous, to be measured is the soybean kernel with high palmitic acid content character of candidate.
In the above method, the place gene in the Map-6064 sites is the 5th article of chromosome of soybean gene group
Glyma.05g015400 genes；The nucleotides sequence of the Glyma.05g015400 genes is classified as the sequence 1 of sequence table；It is described
Map-6064 sites are the 891st of sequence 1 in sequence table.
In the above method, the low palmitic acid content refers to that palmitic acid content is less than 12.3%；The high palmitic acid contains
Amount refers to that palmitic acid content can be more than 12.3%.
In the above method, the method for the genotype based on Map-6064SNP sites in the detection soybean gene group to be measured
It may include：(1) Soybean genomic DNA is extracted.(2) Map-6064 probe groups are added, carry out allele-specific extension and company
Enzyme connection is connect, one section of fragment comprising Map-6064SNP sites and address sequence can be obtained.(3) obtained fragment is carried out
PCR amplification (in amplification system have Cy3 mark dATP, Cy5 mark dGTP, dCTP and dTTP) afterwards with the chip (U.S.
Illumina Products, there is microballon on chip, bead surface is connected with the complementary series of the address sequence) carry out it is miscellaneous
Hand over.(4) chip scanning, according to two kinds of fluorescence color interpretations and genotyping result is exported using software.So that it is determined that soybean product to be measured
Kind is that CC is homozygous or TT is homozygous based on Map-6064SNP loci gene types.
Present invention also offers a kind of product.
Product provided by the present invention, can be in detection soybean gene group polymorphism based on Map-6064SNP sites or
The material of genotype；The function of the product is following (a), (b) or (c)：
(a) identify or aid in the palmitic acid content character of identification soybean kernel；
(b) identify or aid in the related SNP of identification soybean kernel palmitic acid content character；
(c) screening or assisting sifting have the soybean kernel of different palmitic acid contents.
In the said goods, in the soybean gene group genotype based on Map-6064SNP sites can be TT it is homozygous or
CC is homozygous.
In the said goods, the place gene in the Map-6064 sites is the 5th article of chromosome of soybean gene group
Glyma.05g015400 genes；The nucleotides sequence of the Glyma.05g015400 genes is classified as the sequence 1 of sequence table；It is described
Map-6064 sites are the 891st of sequence 1 in sequence table.
In the said goods, the polymorphism detected in soybean gene group based on Map-6064SNP sites or genotype
Material may include Map-6064SNP probe groups.
In the said goods, the palmitic acid content is high palmitic acid content or low palmitic acid content；The high palmitic acid contains
Amount refers to that palmitic acid content can be more than 12.3%；The low palmitic acid content refers to that palmitic acid content is less than 12.3%.
Detect in soybean gene group the material of polymorphism based on Map-6064SNP sites or genotype following (1)-
(6) any application in falls within protection scope of the present invention：
(1) identify or aid in the palmitic acid content character of identification soybean kernel；
(2) product of the palmitic acid content character of identification or auxiliary identification soybean kernel is prepared；
(3) identify or aid in the related SNP of identification soybean kernel palmitic acid content character；
(4) production of the SNP of identification or auxiliary identification soybean kernel palmitic acid content character correlation is prepared
Product；
(5) screening or assisting sifting have the soybean kernel of different palmitic acid contents；
(6) preparation screening or assisting sifting have the product of the soybean kernel of different palmitic acid contents.
In above-mentioned application, in (1) and/or (2), the palmitic acid content character of the soybean kernel can be high palm fibre
Palmitic acid acid content character or low palmitic acid content character.The high palmitic acid content refer to palmitic acid content can be 12.3% with
On；The low palmitic acid content refers to that palmitic acid content is less than 12.3%.
In above-mentioned application, in (3) and/or (4), the palmitic acid content character of the soybean kernel can be high palm fibre
Palmitic acid acid content character or low palmitic acid content character.The high palmitic acid content refer to palmitic acid content can be 12.3% with
On；The low palmitic acid content refers to that palmitic acid content is less than 12.3%.
In above-mentioned application, in (5) and/or (6), the soybean kernel with different palmitic acid contents can be
Soybean kernel with high palmitic acid content character or the soybean kernel with low palmitic acid content character.The high palmitic acid contains
Amount refers to that palmitic acid content can be more than 12.3%；The low palmitic acid content refers to that palmitic acid content is less than 12.3%.
In above-mentioned application, in the soybean gene group genotype based on Map-6064SNP sites can be TT it is homozygous or
CC is homozygous.
In above-mentioned application, the place gene in the Map-6064 sites is the 5th article of chromosome of soybean gene group
Glyma.05g015400 genes；The nucleotides sequence of the Glyma.05g015400 genes is classified as the sequence 1 of sequence table；It is described
Map-6064 sites are the 891st of sequence 1 in sequence table.
It is described to be used to detect the polymorphism or gene based on Map-6064SNP sites in soybean gene group in above-mentioned application
The material of type may include Map-6064SNP probe groups.
Any of the above-described Map-6064SNP probe groups can be made up of probe 1, probe 2 and probe 3：
The probe 1 is single strand dna, and the sequence of probe 1 is as shown in the nucleotides in sequence 2 in sequence table.
The probe 2 is single strand dna, and the sequence of probe 2 is as shown in the nucleotides in sequence 3 in sequence table.
The probe 3 is single strand dna, and the sequence of probe 3 is as shown in the nucleotides in sequence 4 in sequence table.
In the present invention, the low palmitic acid content refers to that palmitic acid content is less than 12.3%；The high palmitic acid content
It can be more than 12.3% to refer to palmitic acid content.
A kind of it is demonstrated experimentally that method identified or aid in identification soybean kernel palmitic acid content character provided by the present invention
It can be used for identifying or aid in identifying the height of soybean kernel palmitic acid content in soybean varieties to be measured.The high palmitic acid content
It is more than 12.3% to refer to palmitic acid content；The low palmitic acid content refers to that palmitic acid content is less than 12.3%.
Embodiment
The present invention is further described in detail with reference to embodiment, the embodiment provided is only for explaining
The bright present invention, the scope being not intended to be limiting of the invention.
Experimental method in following embodiments, it is conventional method unless otherwise specified.
Material used, reagent etc., unless otherwise specified, are commercially obtained in following embodiments.
40 Southern soybean kinds described in following embodiments and 60 northern bean kinds are recorded in the following literature：King
State merit Chinese soybean variety source catalogue Beijing：Chinese agriculture publishing house, 1982；Chang Ru towns, grandson build English Chinese soybean kinds
Resource TOC：Sequel Beijing：Agriculture publishing house, 1991；Chang Ru towns, Sun Jianying, Qiu Lijuan, old dance Chinese soybean kinds
Resource TOC：Sequel two, Beijing：Chinese agriculture publishing house, 1996.
In following embodiments, the low palmitic acid content refers to that palmitic acid content is less than 12.3%；The high palmitic acid
Content refers to that palmitic acid content can be more than 12.3%.
Embodiment 1, Map-6064SNP sites are the SNPs related to soybean kernel palmitic acid content
First, Map-6064 mononucleotide polymorphism sites essential information
Map-6064 mononucleotide polymorphism sites are located at Glyma.05G015400 gene nucleotide series (sequence 1)
891, its essential information is shown in Table 1.
Table 1, Map-6064SNP mononucleotide polymorphism site essential informations
2nd, Map-6064SNP genotype and the association analysis of soybean kernel palmitic acid content
1st, the preparation of Map-6064SNP probe groups
According to the full-length genome SNP data sets of independent development, Map-6064SNP sites and artificial synthesized Map- are screened
6064SNP probe groups：
Probe 1：ACTTCGTCAGTAACGGACGCTGCATACAATGATTGGTTCATGTCA (sequence 2 in sequence table)；
Probe 2：GAGTCGAGGTCATATCGTGCTGCATACAATGATTGGTTCATGTCG (sequence 3 in sequence table)；
Probe 3：
TGTACAATGTATTCTTCACTTCACTAGATCGGTTACTTCTGGTCAGGTGGTCTGCCTATAGTGAGTC
(sequence 4 in sequence table).
The complementary series of address sequence is connected with the microballon of chip.
2nd, the foundation based on Map-6064SNP Genotyping methods
Using Illumina SNP detection platforms to 40 Southern soybean kinds shown in table 2 and 60 shown in table 3
Individual genotype of the northern bean kind based on Map-6064SNP sites is detected.
Detection object is genomic DNA.Using genomic DNA as template, probe 1, probe 2 and spy prepared by step 1 are added
Pin 3, carry out allele-specific extension and connected with ligase, one section can be obtained and include Map-6064SNP sites and address
The fragment of sequence.By obtained fragment enter performing PCR amplification (in amplification system have Cy3 mark dATP, Cy5 mark dGTP,
DCTP and dTTP) (U.S.'s Illumina Products, there is microballon on chip, bead surface is connected with describedly with chip afterwards
The complementary series of location sequence) hybridized.Then chip scanning is carried out, according to two kinds of fluorescence color interpretations and is exported using software
Genotyping result.Determine that soybean varieties to be measured are based on Map-6064SNP loci gene types.
As a result show, it is that CC is homozygous or TT is homozygous that soybean varieties to be measured, which are based on Map-6064SNP loci gene types,.
3rd, Map-6064SNP genotype and the association analysis of soybean kernel palmitic acid content
(1) palmitic acid content of soybean kernel in soybean varieties is detected
In 2010,2011 and 2012 respectively by 60 northern beans in the 40 Southern soybean kinds and table 3 in table 2
Kind is planted in Institute of Crop Science, Chinese Academy of Agricultural Science's Sanya, Hainan base.Tested in field using completely random district's groups
Design, in triplicate.0.4 meter of field planting line width, row is long 1.5 meters, 0.1 meter of strain spacing.After seed harvest, by same year
Part, the content for repeating seed mixing three times, detecting soybean kernel palmitic acid of same kind, then take same kind to detect for 3 years
As a result average value, the palmitic acid content of the kind is obtained, as a result as shown in table 2 and table 3.Palmitic acid content is detected to utilize
HP6890 gas chromatographs (GC) (Agilent Technologies, Palo Alto, CA, USA) are carried out, specific detection method
With reference to Yang et al.Theor Appl Genet (2010) 120:665–678.
(2) methods of genotyping is carried out based on Map-6064SNP sites according to what step 2 was established, detected in table 2 and table 3
Genotype of the soybean varieties based on Map-6064SNP sites, as a result shown in table 2 and table 3.Table 2 is 40 Southern soybean kinds
Based on the genotype in Map-6064SNP sites, table 3 is 60 genotype of the northern bean kind based on Map-6064SNP sites.
2.40 genotype of the Southern soybean kind based on Map-6064SNP sites of table
| Unified number | Variety name | Genotype based on Map-6064SNP sites | Palmitic acid content (%) |
| ZDD11951 | The white soya bean of fan | TT | 12.4 |
| ZDD12023 | Slow soya bean 1 | TT | 12.4 |
| ZDD16282 | Miscellaneous beans -6 | TT | 12.2 |
| ZDD13341 | July is yellow | TT | 12.1 |
| ZDD12400 | October is yellow | TT | 12.1 |
| ZDD12389 | Black eye socket | TT | 11.8 |
| ZDD05494 | Honghu is quick-fried June | TT | 11.8 |
| ZDD24189 | Jiangxi beans 5 | TT | 11.7 |
| ZDD14782 | Long husky mud beans | TT | 11.7 |
| ZDD14783 | Short raw mud beans are 1. | TT | 11.4 |
| ZDD14505 | Yizhang is yellow June | TT | 10.7 |
| ZDD17457 | Sheep eye beans | CC | 13.4 |
| ZDD12845 | Jiange jungle fowl nest beans | CC | 13.4 |
| ZDD12635 | June is early in money | CC | 13.1 |
| ZDD14190 | The white autumn No. 1 | CC | 13.0 |
| ZDD04430 | Taixing short-foot is red | CC | 12.9 |
| ZDD06094 | August is white | CC | 12.9 |
| ZDD21208 | The Zhejiang spring No. 3 | CC | 12.9 |
| ZDD11703 | Dawn soya bean | CC | 12.9 |
| ZDD20532 | Small soya bean | CC | 12.8 |
| ZDD06528 | Yellow green soy bean | CC | 12.8 |
| ZDD12655 | Meishan is quick-fried June | CC | 12.8 |
| ZDD22145 | Big soya bean -2 | CC | 12.8 |
| ZDD12331 | Small white hair | CC | 12.8 |
| ZDD14252 | The early black beans in Fengcheng | CC | 12.8 |
| ZDD12527 | The small soya bean in Pixian County | CC | 12.8 |
| ZDD06454 | Heng Feng crow beans | CC | 12.7 |
| ZDD21030 | Pengshan Huang shell -3 | CC | 12.7 |
| ZDD12386 | Big magnificent beans | CC | 12.7 |
| ZDD20652 | 8307-8-1 | CC | 12.6 |
| ZDD20671 | Tribute beans 7 | CC | 12.6 |
| ZDD16743 | Lianjiang slope soya bean | CC | 12.6 |
| ZDD20676 | June is yellow | CC | 12.6 |
| ZDD14240 | Duchang crow beans | CC | 12.6 |
| ZDD16838 | The big black soya beans of Bao Ting | CC | 12.6 |
| ZDD14125 | Pu's beans 451 | CC | 12.5 |
| ZDD06515 | Hunan beans 4 | CC | 12.5 |
| ZDD06361 | Up to Pu beans | CC | 12.5 |
| ZDD05979 | Five is bluish white | CC | 12.5 |
| ZDD11575 | Pattern beans | CC | 12.5 |
3.60 genotype of the northern bean kind based on Map-6064SNP sites of table
| Unified number | Variety name | Genotype based on Map-6064SNP sites | Palmitic acid content (%) |
| ZDD18845 | Shanxi beans 13 | TT | 12.1 |
| WDD00528 | Amsoy | TT | 12.0 |
| ZDD07391 | Huge rock beans | TT | 12.0 |
| WDD01069 | Biaoge du lot et gerome | TT | 12.0 |
| WDD01307 | Flora | TT | 12.0 |
| ZDD07409 | With imperial oil too | TT | 12.0 |
| WDD00167 | Harosoy | TT | 12.0 |
| ZDD01982 | Its goose egg | TT | 12.0 |
| ZDD08690 | Small soya bean | TT | 11.9 |
| ZDD07370 | Cherish the big grain of moral white flower | TT | 11.9 |
| ZDD01169 | Ox hair is yellow | TT | 11.9 |
| WDD01451 | Nova | TT | 11.9 |
| WDD00001 | Clark | TT | 11.8 |
| ZDD23820 | Distant beans 14 | TT | 11.8 |
| WDD01174 | Salut 216 | TT | 11.8 |
| ZDD18928 | Fen beans 31 | TT | 11.8 |
| ZDD22659 | Close rich 37 | TT | 11.8 |
| WDD00626 | PI196160 | TT | 11.8 |
| ZDD00698 | Dark brown beans | TT | 11.4 |
| ZDD07809 | Iron pod is blue or green | CC | 13.1 |
| ZDD08603 | Small soya bean | CC | 13.1 |
| ZDD00332 | Mo River fodder food beans | CC | 13.1 |
| ZDD22649 | Anti- line 1 | CC | 13.0 |
| WDD01252 | Ten victory come into leaves | CC | 13.0 |
| ZDD22643 | Eastern agriculture 42 | CC | 13.0 |
| ZDD00076 | Peaceful agriculture 1 | CC | 12.9 |
| ZDD06836 | Peaceful agriculture 6 | CC | 12.9 |
| WDD01381 | Dunajka | CC | 12.9 |
| WDD01165 | Amur 41 | CC | 12.9 |
| ZDD07218 | Pale reddish brown No. 2 | CC | 12.8 |
| ZDD22650 | Anti- line 2 | CC | 12.7 |
| ZDD00932 | Big black navel | CC | 12.6 |
| ZDD23684 | Peaceful agriculture 20 | CC | 12.6 |
| ZDD01060 | Yellow navel | CC | 12.6 |
| heinong47 | Black agriculture 47 | CC | 12.6 |
| ZDD23818 | Distant beans 11 | CC | 12.6 |
| ZDD23857 | Cover beans 11 | CC | 12.6 |
| ZDD07244 | Win honour for No. 1 | CC | 12.6 |
| ZDD00854 | Jinzhou 4-1 | CC | 12.6 |
| ZDD07667 | Bear high mountain granule Huang -3 | CC | 12.6 |
| ZDD08650 | The ＞ of soya bean ＜ 2 | CC | 12.5 |
| ZDD00046 | Gram north 1 | CC | 12.5 |
| ZDD18847 | Shanxi beans 15 | CC | 12.5 |
| ZDD01138 | Small soya bean | CC | 12.5 |
| ZDD01629 | The big pea of white navel | CC | 12.5 |
| ZDD06823 | Close rich 25 | CC | 12.4 |
| dongnong163 | Eastern agriculture 163 | CC | 12.4 |
| ZDD22648 | Peaceful agriculture 14 | CC | 12.4 |
| jilin30 | Jilin 30 | CC | 12.4 |
| WDD00570 | Fayette | CC | 12.4 |
| ZDD08643 | Small soya bean | CC | 12.3 |
| ZDD00041 | Heihe No.1 | CC | 12.3 |
| ZDD06819 | Tender rich No. 11 | CC | 12.3 |
| ZDD22642 | Black life 101 | CC | 12.3 |
| ZDD22793 | Jilin 35 | CC | 12.3 |
| ZDD00718 | Tea fodder food beans | CC | 12.3 |
| ZDD06851 | Eastern agriculture 36 | CC | 12.3 |
| WDD00718 | Essex | CC | 12.3 |
| ZDD08705 | Big soya bean<1> | CC | 12.3 |
| ZDD23958 | Handan beans 4 | CC | 12.2 |
The result of table 2 shows that 11 genotype of the kind based on Map-6064SNP sites are TT in 40 soybean south kinds
Homozygous, the average content of the palmitic acid of soybean kernel is 11.8% in the soybean south kind of this 11 kinds；40 soybean
29 genotype of the kind based on Map-6064SNP sites are that CC is homozygous in southern kind, the soybean south of this 29 kinds
The average content of the palmitic acid of soybean kernel is 12.8% in kind.Significance test P<0.01.The result of table 3 is shown, 60 big
19 genotype of the kind based on Map-6064SNP sites are that TT is homozygous in the kind of the beans north, the soybean north of this 19 kinds
The average content of the palmitic acid of soybean kernel is 11.9% in square kind；41 kinds are based on Map- in 60 soybean north kinds
The genotype in 6064SNP sites is that CC is homozygous, and the palmitic acid of soybean kernel is flat in the soybean north kind of this 41 kinds
Equal content is 12.6%.Significance test P<0.01.
Statistical result shows that the genotype based on Map-6064SNP sites is 11 homozygous soybean south kinds of TT
In 19 soybean north kinds, the palmitic acid content of 90% soybean kernel is less than 12.3%；Based on Map-6064SNP sites
Genotype is that the palmitic acid of 99% soybean kernel contains in CC homozygous 29 soybean south kinds and 41 soybean north kinds
Measure as more than 12.3%.
It is demonstrated experimentally that step 2 establish methods of genotyping is carried out based on Map-6064SNP sites can be used for identification and treat
Survey the height of soybean kernel palmitic acid content in soybean varieties：Genotype of the soybean to be measured based on Map-6064SNP sites is detected,
If the genotype based on Map-6064SNP sites is that TT is homozygous, soybean kernel to be measured is candidate in soybean gene group to be measured
The soybean kernel with low palmitic acid content character, if the base based on Map-6064SNP sites in soybean gene group to be measured
Because type is the soybean kernel with high palmitic acid content character that CC is homozygous, soybean kernel to be measured is candidate.The high palm
Acid content refers to that palmitic acid content is more than 12.3%；The low palmitic acid content refers to that palmitic acid content is less than
12.3%.
Claims (7)
1. a kind of method identified or aid in identification soybean kernel palmitic acid content character, comprises the following steps：Detect to be measured big
Genotype based on Map-6064SNP sites in beans genome, if TT is homozygous, soybean kernel to be measured has for candidate's
The soybean kernel of low palmitic acid content character, if CC is homozygous, soybean kernel to be measured for candidate's there is high palmitic acid to contain
Measure the soybean kernel of character；The place gene in the Map-6064SNP sites is the 5th article of chromosome of soybean gene group
Glyma.05G015400 genes；The nucleotides sequence of the Glyma.05G015400 genes is classified as the sequence 1 of sequence table；It is described
Map-6064SNP sites are the 891st of sequence 1 in sequence table；The low palmitic acid content refers to that palmitic acid content is less than
12.3%；The high palmitic acid content refers to that palmitic acid content can be more than 12.3%.
2. a kind of method that screening or assisting sifting have the soybean kernel of different palmitic acid contents, comprises the following steps：Detection
Genotype based on Map-6064SNP sites in soybean gene group to be measured, if TT is homozygous, soybean kernel to be measured is candidate
The soybean kernel with low palmitic acid content character, if CC is homozygous, soybean kernel to be measured has high palm fibre for candidate's
The soybean kernel of palmitic acid acid content character；The place gene in the Map-6064SNP sites is the 5th article of chromosome of soybean gene group
Glyma.05G015400 genes；The nucleotides sequence of the Glyma.05G015400 genes is classified as the sequence 1 of sequence table；It is described
Map-6064SNP sites are the 891st of sequence 1 in sequence table；The low palmitic acid content refers to that palmitic acid content is less than
12.3%；The high palmitic acid content refers to that palmitic acid content can be more than 12.3%.
A kind of 3. product, for the polymorphism or the material of genotype in detection soybean gene group based on Map-6064SNP sites；Institute
The function of stating product is as follows（a）、（b）Or（c）：
（a）Identification or the palmitic acid content character of auxiliary identification soybean kernel；
（b）Identification or the SNP of auxiliary identification soybean kernel palmitic acid content character correlation；
（c）Screening or assisting sifting have the soybean kernel of different palmitic acid contents；
The place gene in the Map-6064SNP sites is the Glyma.05G015400 bases of the 5th article of chromosome of soybean gene group
Cause；The nucleotides sequence of the Glyma.05G015400 genes is classified as the sequence 1 of sequence table；The Map-6064SNP sites are sequence
The 891st of sequence 1 in list.
It is as follows 4. detecting the application of the material of the polymorphism or genotype in soybean gene group based on Map-6064SNP sites
（1）、（2）、（3）、（4）、（5）Or（6）：
（1）Identification or the palmitic acid content character of auxiliary identification soybean kernel；
（2）Prepare the product of the palmitic acid content character of identification or auxiliary identification soybean kernel；
（3）Identification or the SNP of auxiliary identification soybean kernel palmitic acid content character correlation；
（4）Prepare the product of the SNP of identification or auxiliary identification soybean kernel palmitic acid content character correlation；
（5）Screening or assisting sifting have the soybean kernel of different palmitic acid contents；
（6）Preparation screening or assisting sifting have the product of the soybean kernel of different palmitic acid contents；
The place gene in the Map-6064SNP sites is the Glyma.05G015400 bases of the 5th article of chromosome of soybean gene group
Cause；The nucleotides sequence of the Glyma.05G015400 genes is classified as the sequence 1 of sequence table；The Map-6064SNP sites are sequence
The 891st of sequence 1 in list.
5. the application described in product as claimed in claim 3 or claim 4, it is characterised in that：In the soybean gene group
Genotype based on Map-6064SNP sites is that TT is homozygous or CC is homozygous.
6. the application described in product as claimed in claim 3 or claim 4, it is characterised in that：The palmitic acid content is
High palmitic acid content or low palmitic acid content；The high palmitic acid content refers to that palmitic acid content is more than 12.3%；It is described low
Palmitic acid content refers to that palmitic acid content is less than 12.3%.
7. the application described in product as claimed in claim 3 or claim 4, it is characterised in that：It is described to be used to detect soybean
The material of polymorphism or genotype based on Map-6064 SNP sites in genome includes Map-6064SNP probe groups；
The Map-6064SNP probe groups are made up of probe 1, probe 2 and probe 3；
The probe 1 is the single strand dna shown in sequence 2 in sequence table；
The probe 2 is the single strand dna shown in sequence 3 in sequence table；
The probe 3 is the single strand dna shown in sequence 4 in sequence table.
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101500403A (en) * | 2006-03-10 | 2009-08-05 | 孟山都技术有限责任公司 | Soybean seed and oil compositions and methods of making same |
| US8710295B2 (en) * | 2010-05-10 | 2014-04-29 | Pioneer Hi-Bred International Inc | Soybean sequences associated with the FAP3 locus |
-
2015
- 2015-07-23 CN CN201510437818.4A patent/CN105132531B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101500403A (en) * | 2006-03-10 | 2009-08-05 | 孟山都技术有限责任公司 | Soybean seed and oil compositions and methods of making same |
| US8710295B2 (en) * | 2010-05-10 | 2014-04-29 | Pioneer Hi-Bred International Inc | Soybean sequences associated with the FAP3 locus |
Non-Patent Citations (4)
| Title |
|---|
| Molecular Analysis of Soybean Lines with Low Palmitic Acid Content in the Seed Oil;Andrea J.Cardinal等;《CROP SCIENCE》;20070206;第47卷;第304-310页 * |
| SSR- and SNP-related QTL underlying linolenic acid and other fatty acid contents in soybean seeds across multiple environments;Dongwei Xie等;《Mol Breeding》;20110706;第30卷;第169-179页 * |
| 大豆种质脂肪酸主要组分鉴定与QTL标记定位;于福宽;《中国优秀硕士学位论文全文数据库 农业科技辑》;20111015(第10期);D047-141 * |
| 大豆脂肪酸组成性状全基因组关联分析;韩世凤;《中国优秀硕士学位论文全文数据库 农业科技辑》;20140815(第8期);D047-199 * |
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