CN105132402A - Preparation method of microbial macromolecular balls for nitrogen removal from wastewater - Google Patents
Preparation method of microbial macromolecular balls for nitrogen removal from wastewater Download PDFInfo
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- CN105132402A CN105132402A CN201510425272.0A CN201510425272A CN105132402A CN 105132402 A CN105132402 A CN 105132402A CN 201510425272 A CN201510425272 A CN 201510425272A CN 105132402 A CN105132402 A CN 105132402A
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Abstract
The invention discloses a preparation method of microbial macromolecular balls for nitrogen removal from wastewater, belonging to the technical field of wastewater treatment. The preparation method comprises the following steps: adding distilled water into polyvinyl alcohol powder, and starting an electric stirrer for stirring the distilled water and the polyvinyl alcohol powder for reacting uniformly at certain temperature; sufficiently cooling a mixture formed by a reaction product of distilled water and the polyvinyl alcohol powder and a reaction product of sodium alginate and silicon dioxide; carrying out crosslinking fixation on a mixed substance formed by the sufficiently cooled mixture and aluminum sulfate solution and boric acid solution which are added into the sufficiently cooled mixture; feeding mixed substance subjected to crosslinking fixation into a spherical mold, and then extruding the substance from an extruder; drying in an oven and placing in a muffle furnace for calcining; washing by adopting alcohol and deionized water; and drying, thus preparing the microbial macromolecular balls for nitrogen removal from wastewater. The preparation method has the beneficial effects that the prepared microbial macromolecular balls have the characteristics of high density and high nitrogen resistance, and are suitable for treatment on wastewater with ammonia-nitrogen content being greater than 400mg/L; assimilation is realized after adsorption for ammonia and nitrogen, and microbes can grow again; and as the inside microbes are in the anaerobic state, ammonia and nitrogen can be removed completely, and the removal rate reaches 99.8% or above.
Description
Technical field
The present invention relates to a kind of microorganism polymer bead preparation method of denitrogenation of waste water, belong to technical field of sewage.
Background technology
Microorganism polymer bead technology of preparing is a kind of novel biotechnology, refer to by chemistry or physical method by microorganism or electrodes method in the area of space limited, and make it keep active and can the technology of Reusability; Microorganism polymer bead can be fixed complete microorganism cells, avoid the activity of artificial destruction biological enzyme and the stability of biochemical reaction, microorganism cells density in unit volume medium can be improved, and microorganism stay active for long periods can be made, in addition, the microenvironment of its cell granulations is conducive to shielding indigenous bacterium, phage and toxicant to the harmful competition of microbe, murder by poisoning, and also the performance of Absorbable organic halogens is high-effect in complex environment to make it.
Summary of the invention
Technical problem to be solved by this invention is: can only remove the waste water that ammonia-nitrogen content is less than 400mg/L for microbial sludge, when content is greater than 400mg/L, microorganism can kill by ammonia nitrogen substances, cause the drawback that active sludge lost efficacy, provide a kind of microorganism polymer bead preparation method of denitrogenation of waste water, the microorganism polymer bead that the method is obtained, has the feature of high-density, high patience, can process the waste water that ammonia-nitrogen content is greater than 400mg/L.
For solving the problems of the technologies described above, the present invention adopts technical scheme as described below: a kind of microorganism polymer bead preparation method of denitrogenation of waste water, and its concrete preparation process is:
(1) pva powder getting 20 ~ 30g, in triangle round flask, adds 50 ~ 100mL distilled water, and temperature is 30 DEG C ~ 40 DEG C, starts electric mixer, stirring reaction 10 ~ 15min;
(2) be warming up to 45 DEG C ~ 65 DEG C, after completing, respectively the sodium alginate of 10 ~ 13g and the silicon-dioxide of 1 ~ 5g joined in Erlenmeyer flask, stir, stop after reaction 1 ~ 2h;
(3) frozen water said mixture being placed in 450 ~ 550mL fully cools, alum liquor and 15 ~ 30mL boric acid solution of 15 ~ 30mL is slowly dripped when stirring, temperature in reactor controls, in normal temperature state, after dropwising, then to stir 20 ~ 30min;
(4) and then under nitrogen protection condition, thermostatic control is carried out crosslinkedly fixing 5 ~ 8h under 40 DEG C ~ 60 DEG C conditions;
(5) material after crosslinked fixing is sent in spherical die, then extrude in forcing machine, in coccoid;
(6) coccoid material being put into temperature is that at 60 DEG C ~ 70 DEG C, baking oven is dried, and is placed in retort furnace, under temperature is 500 DEG C ~ 600 DEG C conditions, calcine 2 ~ 3h;
(7) by the material ethanol after calcining and washed with de-ionized water 2 ~ 3 times, at temperature is 35 DEG C ~ 45 DEG C, dry 30 ~ 60min, namely obtains a kind of microorganism polymer bead of denitrogenation of waste water.
Described forcing machine extrusion temperature is: feed zone 110 DEG C ~ 130 DEG C, 140 DEG C ~ 160 DEG C, compression section, fluxing zone 160 DEG C ~ 190 DEG C.
Application method of the present invention: the microorganism polymer bead prepared is placed in the device of cylindrical pond as filler, depth of packing is 2 ~ 4cm, one deck is laid every 30cm, until Chi Ding, then passing into containing mass concentration is the ammonia nitrogen waste water of 400 ~ 800mg/L, reaction 3h, detects the content of ammonia nitrogen in waste water.
Principle of the present invention: the microorganism polymer bead that the method is prepared into, it can be adapted to propagate microorganisms, form the coenosis of high-density, high resistance to nitrogen, because microorganism polymer bead appearance is close to air, assimilate after absorption ammonia nitrogen, microorganism is grown, and inner microorganism is in anaerobic state, make to carry out digestion and instead digest between the aerobic and anaerobism of ammonia nitrogen, the carrying out of ammonia nitrogen in waste water is removed.
The present invention is compared with additive method, and Advantageous Effects is:
(1) the microorganism polymer bead that the method is obtained has the feature such as high-density, high resistance to nitrogen, is applicable to the wastewater treatment that ammonia-nitrogen content is greater than 400mg/L;
(2) assimilate after absorption ammonia nitrogen, the regrowth of microorganism energy;
(3) because inner microorganism is in anaerobic state, make ammonia nitrogen between aerobic and anaerobism, remove more thorough.
Embodiment
The pva powder getting 20 ~ 30g, in triangle round flask, adds 50 ~ 100mL distilled water, and temperature is 30 DEG C ~ 40 DEG C, starts electric mixer, stirring reaction 10 ~ 15min; Be warming up to 45 DEG C ~ 65 DEG C, after completing, respectively the sodium alginate of 10 ~ 13g and the silicon-dioxide of 1 ~ 5g joined in Erlenmeyer flask, stir, stop after reaction 1 ~ 2h; Frozen water said mixture being placed in 450 ~ 550mL fully cools, alum liquor and 15 ~ 30mL boric acid solution of 15 ~ 30mL is slowly dripped when stirring, temperature in reactor controls, in normal temperature state, after dropwising, then to stir 20 ~ 30min; And then under nitrogen protection condition, thermostatic control is carried out crosslinked fixing 5 ~ 8h under 40 DEG C ~ 60 DEG C conditions; Material after crosslinked fixing is sent in spherical die, then extrudes in forcing machine, in coccoid; Coccoid material being put into temperature is that at 60 DEG C ~ 70 DEG C, baking oven is dried, and is placed in retort furnace, under temperature is 500 DEG C ~ 600 DEG C conditions, calcine 2 ~ 3h; Finally by the material ethanol after calcining and washed with de-ionized water 2 ~ 3 times, at temperature is 35 DEG C ~ 45 DEG C, dry 30 ~ 60min, namely obtains a kind of microorganism polymer bead of denitrogenation of waste water.
Described forcing machine extrusion temperature is: feed zone 110 DEG C ~ 130 DEG C, 140 DEG C ~ 160 DEG C, compression section, fluxing zone 160 DEG C ~ 190 DEG C.
Example 1
First the pva powder getting 20g, in triangle round flask, adds 50mL distilled water, and temperature is 30 DEG C, starts electric mixer, stirring reaction 10min; Be warming up to 45 DEG C, after completing, respectively the sodium alginate of 10g and the silicon-dioxide of 1g joined in Erlenmeyer flask, stir, stop after reaction 1h; Frozen water said mixture being placed in 450mL fully cools, and slowly drip alum liquor and the 15mL boric acid solution of 15mL when stirring, the temperature in reactor controls, in normal temperature state, after dropwising, then to stir 20min; And then under nitrogen protection condition, crosslinked fixing 5h is carried out in thermostatic control under 40 DEG C of conditions; Material after crosslinked fixing is sent in spherical die, then extrudes in forcing machine, in coccoid; Coccoid material being put into temperature is that at 60 DEG C, baking oven is dried, and is placed in retort furnace, under temperature is 500 DEG C of conditions, calcines 2h; Finally by the material ethanol after calcining and washed with de-ionized water 2 times, dry 30min at temperature is 35 DEG C, namely obtains a kind of microorganism polymer bead of denitrogenation of waste water; The microorganism polymer bead prepared is placed in the device of cylindrical pond as filler, depth of packing is 2cm, one deck is laid every 30cm, until Chi Ding, then passing into containing mass concentration is the ammonia nitrogen waste water of 400mg/L, reaction 3h, the content detecting ammonia nitrogen in waste water is reduced to 0.5mg/L, and clearance reaches 99.88%.
Example 2
First the pva powder getting 25g, in triangle round flask, adds 75mL distilled water, and temperature is 35 DEG C, starts electric mixer, stirring reaction 12min; Be warming up to 55 DEG C, after completing, respectively the sodium alginate of 12g and the silicon-dioxide of 3g joined in Erlenmeyer flask, stir, stop after reaction 1.5h; Frozen water said mixture being placed in 500mL fully cools, and slowly drip alum liquor and the 22mL boric acid solution of 25mL when stirring, the temperature in reactor controls, in normal temperature state, after dropwising, then to stir 25min; And then under nitrogen protection condition, crosslinked fixing 6.5h is carried out in thermostatic control under 50 DEG C of conditions; Material after crosslinked fixing is sent in spherical die, then extrudes in forcing machine, in coccoid; Coccoid material being put into temperature is that at 65 DEG C, baking oven is dried, and is placed in retort furnace, under temperature is 550 DEG C of conditions, calcines 2.5h; Finally by the material ethanol after calcining and washed with de-ionized water 2 times, dry 45min at temperature is 40 DEG C, namely obtains a kind of microorganism polymer bead of denitrogenation of waste water; The microorganism polymer bead prepared is placed in the device of cylindrical pond as filler, depth of packing is 3cm, one deck is laid every 30cm, until Chi Ding, then passing into containing mass concentration is the ammonia nitrogen waste water of 600mg/L, reaction 3h, the content detecting ammonia nitrogen in waste water is reduced to 0.6mg/L, and clearance reaches 99.9%.
Example 3
First the pva powder getting 30g, in triangle round flask, adds 100mL distilled water, and temperature is 40 DEG C, starts electric mixer, stirring reaction 15min; Be warming up to 65 DEG C, after completing, respectively the sodium alginate of 13g and the silicon-dioxide of 5g joined in Erlenmeyer flask, stir, stop after reaction 2h; Frozen water said mixture being placed in 550mL fully cools, and slowly drip alum liquor and the 30mL boric acid solution of 30mL when stirring, the temperature in reactor controls, in normal temperature state, after dropwising, then to stir 30min; And then under nitrogen protection condition, crosslinked fixing 8h is carried out in thermostatic control under 60 DEG C of conditions; Material after crosslinked fixing is sent in spherical die, then extrudes in forcing machine, in coccoid; Coccoid material being put into temperature is that at 70 DEG C, baking oven is dried, and is placed in retort furnace, under temperature is 600 DEG C of conditions, calcines 3h; Finally by the material ethanol after calcining and washed with de-ionized water 3 times, dry 60min at temperature is 45 DEG C, namely obtains a kind of microorganism polymer bead of denitrogenation of waste water; The microorganism polymer bead prepared is placed in the device of cylindrical pond as filler, depth of packing is 4cm, one deck is laid every 30cm, until Chi Ding, then passing into containing mass concentration is the ammonia nitrogen waste water of 800mg/L, reaction 3h, the content detecting ammonia nitrogen in waste water is reduced to 0.4mg/L, and clearance reaches 99.95%.
Claims (2)
1. a microorganism polymer bead preparation method for denitrogenation of waste water, is characterized in that concrete preparation process is:
(1) pva powder getting 20 ~ 30g, in triangle round flask, adds 50 ~ 100mL distilled water, and temperature is 30 DEG C ~ 40 DEG C, starts electric mixer, stirring reaction 10 ~ 15min;
(2) be warming up to 45 DEG C ~ 65 DEG C, after completing, respectively the sodium alginate of 10 ~ 13g and the silicon-dioxide of 1 ~ 5g joined in Erlenmeyer flask, stir, stop after reaction 1 ~ 2h;
(3) frozen water said mixture being placed in 450 ~ 550mL fully cools, alum liquor and 15 ~ 30mL boric acid solution of 15 ~ 30mL is slowly dripped when stirring, temperature in reactor controls, in normal temperature state, after dropwising, then to stir 20 ~ 30min;
(4) and then under nitrogen protection condition, thermostatic control is carried out crosslinkedly fixing 5 ~ 8h under 40 DEG C ~ 60 DEG C conditions;
(5) material after crosslinked fixing is sent in spherical die, then extrude in forcing machine, in coccoid;
(6) coccoid material being put into temperature is that at 60 DEG C ~ 70 DEG C, baking oven is dried, and is placed in retort furnace, under temperature is 500 DEG C ~ 600 DEG C conditions, calcine 2 ~ 3h;
(7) by the material ethanol after calcining and washed with de-ionized water 2 ~ 3 times, at temperature is 35 DEG C ~ 45 DEG C, dry 30 ~ 60min, namely obtains a kind of microorganism polymer bead of denitrogenation of waste water.
2. the microorganism polymer bead preparation method of a kind of denitrogenation of waste water according to claim 1, is characterized in that forcing machine extrusion temperature is: feed zone 110 DEG C ~ 130 DEG C, 140 DEG C ~ 160 DEG C, compression section, fluxing zone 160 DEG C ~ 190 DEG C.
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CN105925560A (en) * | 2016-06-29 | 2016-09-07 | 华南理工大学 | Embedded nano iron/two microbial agents and preparation method thereof |
Citations (1)
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CN103374561A (en) * | 2012-04-13 | 2013-10-30 | 南京科盛环保科技有限公司 | Preparation and water treatment using method of immobilized nitrifying bacteria |
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CN103374561A (en) * | 2012-04-13 | 2013-10-30 | 南京科盛环保科技有限公司 | Preparation and water treatment using method of immobilized nitrifying bacteria |
Non-Patent Citations (2)
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卢徐节等: "固定化微生物技术对养殖水体脱氮的研究进展", 《环境保护与循环经济》 * |
宋超鹏等: "脱氮微生物固定化材料的研究进展", 《常州大学学报(自然科学版)》 * |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN105925560A (en) * | 2016-06-29 | 2016-09-07 | 华南理工大学 | Embedded nano iron/two microbial agents and preparation method thereof |
CN105925560B (en) * | 2016-06-29 | 2020-06-19 | 华南理工大学 | Embedded nano-iron/two microbial agents and preparation method thereof |
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