CN105123674A - Section position using method of low concentration NAA gel - Google Patents

Abstract

The invention discloses a section position using method of a low concentration NAA gel and belongs to the technical field of plant biology. The method comprises: firstly preparing 1mg/mL NNA mother liquor; diluting the mother liquor to 10 mg/L low concentration NAA working liquor; preparing low concentration NNA gel sealing liquor containing sodium carboxymethylcellulose with the mass concentration of 1% and sodium alginate with the mass concentration of 8% by using the working liquor; inserting branches into a culture medium; and dropwise adding the gel sealing liquor into axillary buds of healthy and robust branches.

Description

A kind of joint position using method of low concentration NAA gel

Technical field

A joint position using method for low concentration NAA gel, belongs to plant biotechnology field.

Background technology

Cottage propagation is one of vegetative common method of plant, especially trees, and the method has that cost is low, instant effect, cycle short, used branch abundance and can keep the plurality of advantages such as parent's merit.Usually can use dissimilar hormone during cottage propagation, as NAA, IBA, NAA, 2,4-D, GA ₃, 6-BA, KT, TDZ, TIBA, ethrel, B ₉, PP333, MH etc., the interpolation of above-mentioned hormone can enable plant at body or in vitro ahead of time or postpone to take root, bloom, bear fruit, and increases radical, induction axillary bud sprouting, improves percentage of fertile fruit etc., agricultural production plays important purposes.But the branch being difficult to break up in in-vitro propagate or rooting process, due to the loss of long moisture or nutriment, extremely easily causes death.And the method that existing employing urges root to lure bud to bloom possesses skills the shortcomings such as step is more, cost is high, unstable, in production, effect is unsatisfactory.Therefore, need badly a kind of fast and convenient method for plant axillary bud deriving, take root and Cheng Hua etc.

Summary of the invention

A joint position using method for low concentration NAA gel, is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel, cuttage, the dropping of axillalry bud place in described method, concrete operation step is as follows:

1. hormone mother liquor: precise weight be 0.1g NAA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the NAA mother liquor of 1mg/mL;

2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:100 the low concentration hormone working solution that concentration is 10mg/L;

3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate, dissolves for subsequent use with the hormone working solution that the concentration of 100mL is 10mg/L under 50 DEG C of water bath condition;

cuttage: branch is inserted in cultivation matrix;

axillalry bud place drips: drip gel confining liquid at the axillalry bud place of branch.

The invention has the advantages that from the viewpoint of water conservation, short root, lure bud, bloom, bear fruit, with hormone working solution for solvent, sodium carboxymethylcellulose and sodium alginate are gel carrier, are mixed with containing gel solution that is dissimilar, concentration hormone.Features such as during use, branch, flower being soaked in slightly above-mentioned solution or above-mentioned solution is dripped at axillalry bud place, film can be formed rapidly after gel drying, reaching the evaporation of minimizing moisture, being continuously applied hormone, be easy and simple to handle.

embodiment:

Below in conjunction with specific embodiment, the present invention is conducted further description.

Embodiment

A joint position using method for low concentration NAA gel, is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel, cuttage, the dropping of axillalry bud place in described method, concrete operation step is as follows:

1. hormone mother liquor: precise weight be 0.1g NAA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the NAA mother liquor of 1mg/mL;

2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:100 the low concentration hormone working solution that concentration is 10mg/L;

3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate, dissolves for subsequent use with the hormone working solution that the concentration of 100mL is 10mg/L under 50 DEG C of water bath condition;

cuttage: pot marigold branch is inserted in cultivation matrix;

axillalry bud place drips: drip gel confining liquid at the axillalry bud place of branch, after 20-30 days, axillary bud sprouting rate reaches more than 90%, and branch rooting rate reaches more than 80%.

Claims (1)

1. a joint position using method for low concentration NAA gel, is characterized in that, include hormone mother liquor, working solution preparation, the preparation of hormone gel, cuttage, the dropping of axillalry bud place in described method, concrete operation step is as follows:

1. hormone mother liquor: precise weight be 0.1g NAA with a little 95% ethanol dissolve after, be settled to 100mL with distilled water, be the NAA mother liquor of 1mg/mL;

2. working solution preparation: above-mentioned mother liquor is diluted to according to the ratio of volume ratio 1:100 the low concentration hormone working solution that concentration is 10mg/L;

3. hormone gel preparation: accurately take 1g sodium carboxymethylcellulose and 8g sodium alginate, dissolves for subsequent use with the hormone working solution that the concentration of 100mL is 10mg/L under 50 DEG C of water bath condition;

cuttage: branch is inserted in cultivation matrix;

axillalry bud place drips: drip gel confining liquid at the axillalry bud place of branch.