CN105112404A - Preparation method of nucleic acid extracting solution for animal tissues - Google Patents
Preparation method of nucleic acid extracting solution for animal tissues Download PDFInfo
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Abstract
A preparation method of a nucleic acid extracting solution for animal tissues comprises the following steps: preparation of magnetic microspheres, surface activation and preparation of a final extracting solution, wherein the magnetic microspheres are coated with nanometer magnetic powder through polycarbosilane at first, and the nucleic acid extracting solution is obtained through heat treatment and magnetic separation. The extracting solution prepared by the preparation method can quickly dissociate animal tissues, efficiently separate and purify DNA and be widely applied to the animal disease detection field.
Description
Technical field
The present invention relates to the preparation method of a kind of animal tissues nucleic acid extraction liquid.
Background technology
The food safety of Animal diseases on livestock industry and the mankind has important impact, adopts molecular biology method to carry out diagnosis to animal tissues and can obtain result the most accurately.Committed step is from animal tissues's sample, to extract the DNA of bacterium or virus.Can obtain the DNA of different types of DNA of bacteria or different sorts virus, and genomic integrity, purity and concentration, be from animal tissues, extract the primary goal that DNA diagnoses for animal pathogen.
Usually pollution is had in existing DNA extraction process, and complex steps, the leaching process time is longer.As extracting method such as sodium perchlorate method, SDS method and Wyler's processs, but these methods need sample size larger, and the organism such as phenol are easily residual in DNA solution, particularly amplification procedure can be disturbed in follow-up pcr amplification process, affect amplification, the application of the method is subject to certain restrictions.
Magnetic microsphere, can fast separating and purifying nucleic acid DNA/RNA under the effect of additional magnetic force, easily is automated because of safety and develops rapidly.Magnetic microsphere method for extracting nucleic acid refers to the magnetic microsphere of superparamagnetism adsorbs nucleic acid in high salt, low PH solution, and nucleic acid is carried out the method for nucleic acid extraction from the principle that magnetic microsphere surface departs from low salt solutions.
Existing magnetic microsphere is normally by the spherical particle with certain magnetic of the Material claddings such as the magnetic core such as Z 250 or ferric oxide and silicon-dioxide.Chinese invention patent (CN1217352) discloses a kind of preparation method of magnetic microsphere of coated with silica Z 250, and it adopts acidization at Z 250 surface deposition silicon-dioxide.And the acidic substance added (HCl) can react with magnetic core Z 250 to a certain extent, thus uncertain impact can be produced on the magnetic of magnetic microsphere, cause unstable product quality.
Summary of the invention
The object of the invention is, for some and certain problem of existing in above-mentioned background technology, to provide the preparation method of a kind of animal tissues nucleic acid extraction liquid.
According to the preparation method of animal tissues of the present invention nucleic acid extraction liquid, comprise the following steps:
(1) preparation of magnetic microsphere
(1) by Polycarbosilane dissolution of solid in organic benzene, organic kind solvent, formed emulsion; Joined by nano-scale magnetic powder in the organic alcohols solvent containing tensio-active agent, stir formation suspension; Suspension is joined in emulsion, stir, obtain mixed solution; Wherein the mass ratio of Polycarbosilane and magnetic powder is 2-5:1;
(2) be added dropwise in the warm water solution containing linking agent by microporous membrane by mixed solution, water temperature remains on 60-80 DEG C; Make Polycarbosilane crosslinking curing in mixed solution surperficial at magnetic powder; Utilize underpressure distillation means moisture and organic solvent to be removed, obtain the microspheres with solid of Polycarbosilane coated magnetic powder;
(3) microspheres with solid is carried out insulation 1-2 hour at 500-1000 DEG C, under nitrogen or protection of inert gas, after Polycarbosilane cracking, form the microspheres with solid of porous surface;
(4) microspheres with solid after thermal treatment is screened by Magneto separate means, filter out qualified finished product magnetic microsphere;
(2) activation of magnetic microsphere
Finished product magnetic microsphere, catalyzer and amino agents obtained in step () are mixed with the mass ratio of 1:1-20:5-30, maintenance pH value is 10-13, temperature is at 50-80 DEG C, stirring reaction 5-10 hour, cooling, through magnetic resolution, washing, obtain the magnetic microsphere of surface with affinity groups; Wherein catalyzer is selected from sodium hydroxide or potassium hydroxide, and amino agents is selected from amino-benzene carbonamidine or aminophenyl boronic acid.
(3) preparation of extracting solution
Magnetic microsphere after activation in step (two) and lsothiocyanates, Guanidinium hydrochloride, sodium iodide, EDTA, dodecylamino acid are configured to extracting solution, wherein the concentration of lsothiocyanates is 3.5-5M, the concentration of Guanidinium hydrochloride is 1.5-3M, the concentration of sodium iodide is 1.2-2.5M, the concentration of EDTA is 15-30mM, the concentration of dodecylamino acid is 60-100mM, and the mass concentration of magnetic microsphere is 10-15%.
In (1) of step (), organic benzene, organic kind solvent is selected from the one or more combination in toluene, dimethylbenzene and Vinylstyrene.The mass ratio of Polycarbosilane and organic benzene, organic kind solvent preferably controls at 1-1.5:1, and in this ratio range, the emulsion of formation can keep moderate viscosity, so that follow-up mixing.Organic alcohols solvent selected from ethanol or ethylene glycol; Tensio-active agent is selected from dioctyl succinate disulfonate acid, ten sodium alkyl sulfates, bromination ten alkyl trimethyl ammonium or bromination ten alkylammonium.Above-mentioned tensio-active agent can dissolve each other with Organic Alcohol, thus nano-magnetic powder fully can be disperseed and suspend.Wherein magnetic powder is iron, cobalt, nickel or their alloy, also can be Fe
3o
4.The particle diameter of magnetic powder is preferably 1nm-30nm, and the magnetic powder in this particle size range has superparamagnetism, and namely after externally-applied magnetic field removes, magnetic powder without remanent magnetism, thus can not be assembled.The mass ratio of magnetic powder and organic alcohols solvent is preferably 1-3:1, and the mass ratio of tensio-active agent and organic alcohols solvent is preferably 0.05-0.2:1.Under these conditions of mixture ratios, magnetic powder can not only disperse well, and has relatively high concentration, thus the guarantee provided for the magnetic intensity of the finished product.
In (2) of step (), linking agent is selected from N,N methylene bis acrylamide or ammonia third pyridine, and these two kinds of linking agents can dissolve each other with water, and the mass ratio of linking agent and water is preferably 0.05-0.2:1.In this step, the aperture of microporous membrane is preferably 1-50 μm, is more preferably 10-30 μm.After entering warm water solution by the drop of microporous membrane, form the oil-in-water globosity of size uniformity, and the Polycarbosilane of surface with rounded structures is under linking agent and temperature promoter action, rapidly crosslinking curing occurs, thus evenly envelopes magnetic powder.
In (3) of step (), heat-treat microspheres with solid, be coated on the Polycarbosilane generation cracking on magnetic powder surface, some small molecules volatile hydrocarbons are gone out, thus form porous coating.Wherein temperature rise rate is particularly crucial, and according to common thermal treatment temperature rise rate (5-30 DEG C/min), the rate of cleavage of Polycarbosilane is too fast, and foaming and structural breakdown will occur.The present inventor is when adopting the ultralow temperature rise rate of 0.1-2 DEG C/min, the cracking of unexpected discovery Polycarbosilane is slowly carried out, thus forming the ultramicropore being mainly less than 0.01 μm aperture, this has very important effect to adsorbs nucleic acid in magnetic microsphere application process.By thermal treatment, form the porous coating that main component is silicon carbide after Polycarbosilane cracking, not only there is larger specific surface area, there is higher structural strength simultaneously, and be combined closely with magnetic powder.
In (4) of step (), adopt Magneto separate means, the magnetic microsphere obtained is screened, remove the defective magnetic microsphere not having magnetic or magnetic to require not, finally obtain the qualified magnetic microsphere finished product meeting magnetic requirements.
By the activation treatment of step (two), make magnetic microsphere surface bonding have the active group of carboxyl and hydroxyl, make it have specific adsorption ability to nucleic acid.
In step (three), the compositions such as the lsothiocyanates in extracting solution, Guanidinium hydrochloride, sodium iodide can to animal tissues carry out efficient solution from, dissociate nucleic acid DNA/RNA, nucleoprotein and salinity.Described animal tissues comprises heart, liver, lungs, spleen or kidney, also can be whole blood, blood plasma, serum or animal secretions.
Extracting solution obtained by preparation in accordance with the present invention, can dissociate fast and high efficiency separation purification DNA to animal tissues, can be widely applied to animal epidemic (as bird flu, foot and mouth disease, swine fever, blue otopathy etc.) detection field.
Embodiment
The preparation method of animal tissues of the present invention nucleic acid extraction liquid is described below by specific embodiment.It will be appreciated by those skilled in the art that embodiment described below is only to exemplary illustration of the present invention, but not for making any restriction to it.
Embodiment 1
Take Polycarbosilane solid 1000g, pulverize last, join and fill in the rotary evaporation bottle of 1000g toluene, rotate at water-bath 40 DEG C and dissolve formation emulsion.In beaker, first add the ethanol of 300g, then add the dioctyl succinate disulfonate acid of 20g, after stirring, the nanometer iron powder (particle diameter is below 30nm) of 500g is joined in beaker, stirs 10 minutes, form suspension.The suspension of preparation is slowly joined in the emulsion of preparation, stirs, obtain mixed solution.In collection container, add 1000g deionized water, and add the N,N methylene bis acrylamide of 100g, be heated to 80 DEG C.Above collection container, arrange microporous membrane, the aperture of microporous membrane is 10 μm.Mixed solution is added dropwise in collection container by microporous membrane.Utilize underpressure distillation means the moisture in collection container and organic solvent to be removed, obtain the microspheres with solid of Polycarbosilane coated magnetic powder.Be put into by microspheres with solid in heat treatment furnace, pass into argon shield, be raised to 800 DEG C with the temperature rise rate of 0.5 DEG C/min, be incubated after 1 hour, Temperature fall, obtains the microspheres with solid of porous surface.Utilize the magnetic magnetic microsphere of magnet sucking-off tool, obtain the finished product magnetic microsphere that 568g is qualified.
Obtained finished product magnetic microsphere and sodium hydroxide and amino-benzene carbonamidine are configured to the aqueous solution with the mass ratio of 1:3:8, and keep pH value to be 10.8, temperature is at 80 DEG C, stirring reaction 5 hours, cooling, through magnetic resolution, washing, obtains the magnetic microsphere of surface with affinity groups.
Magnetic microsphere after activation and lsothiocyanates, Guanidinium hydrochloride, sodium iodide, EDTA, dodecylamino acid are configured to extracting solution, wherein the concentration of lsothiocyanates is 4M, the concentration of Guanidinium hydrochloride is 1.8M, the concentration of sodium iodide is 1.2M, the concentration of EDTA is 18mM, the concentration of dodecylamino acid is 60mM, and the mass concentration of magnetic microsphere is 12%.
Embodiment 2
Take Polycarbosilane solid 1000g, pulverize last, join and fill in the rotary evaporation bottle of 800g Vinylstyrene, rotate at water-bath 60 DEG C and dissolve formation emulsion.In beaker, first add the ethylene glycol of 300g, then add the bromination ten alkyl trimethyl ammonium of 30g, after stirring, the nano-cobalt powder (particle diameter is below 30nm) of 300g is joined in beaker, stirs 10 minutes, form suspension.The suspension of preparation is slowly joined in the emulsion of preparation, stirs, obtain mixed solution.In collection container, add 1000g deionized water, and add the N,N methylene bis acrylamide of 100g, be heated to 60 DEG C.Above collection container, arrange microporous membrane, the aperture of microporous membrane is 30 μm.Mixed solution is added dropwise in collection container by microporous membrane.Utilize underpressure distillation means the moisture in collection container and organic solvent to be removed, obtain the microspheres with solid of Polycarbosilane coated magnetic powder.Be put into by microspheres with solid in heat treatment furnace, pass into argon shield, be raised to 1000 DEG C with the temperature rise rate of 0.2 DEG C/min, be incubated after 1 hour, Temperature fall, obtains the microspheres with solid of porous surface.Utilize the magnetic magnetic microsphere of magnet sucking-off tool, obtain the finished product magnetic microsphere that 324g is qualified.
Obtained finished product magnetic microsphere and potassium hydroxide and aminophenyl boronic acid are configured to the aqueous solution with the mass ratio of 1:5:10, and keep pH value to be 12.5, temperature is at 60 DEG C, stirring reaction 10 hours, cooling, through magnetic resolution, washing, obtains the magnetic microsphere of surface with affinity groups.
Magnetic microsphere after activation and lsothiocyanates, Guanidinium hydrochloride, sodium iodide, EDTA, dodecylamino acid are configured to extracting solution, wherein the concentration of lsothiocyanates is 5M, the concentration of Guanidinium hydrochloride is 2.5M, the concentration of sodium iodide is 2.0M, the concentration of EDTA is 30mM, the concentration of dodecylamino acid is 80mM, and the mass concentration of magnetic microsphere is 15%.
Embodiment 3
Take Polycarbosilane solid 1000g, pulverize last, join and fill in the rotary evaporation bottle of 1000g dimethylbenzene, rotate at water-bath 50 DEG C and dissolve formation emulsion.In beaker, first add the ethanol of 300g, then add ten sodium alkyl sulfates of 45g, after stirring, by the nanometer Fe of 300g
3o
4powder (particle diameter is below 30nm) joins in beaker, stirs 10 minutes, forms suspension.The suspension of preparation is slowly joined in the emulsion of preparation, stirs, obtain mixed solution.In collection container, add 1000g deionized water, and add ammonia third pyridine of 100g, be heated to 80 DEG C.Above collection container, arrange microporous membrane, the aperture of microporous membrane is 50 μm.Mixed solution is added dropwise in collection container by microporous membrane.Utilize underpressure distillation means the moisture in collection container and organic solvent to be removed, obtain the microspheres with solid of Polycarbosilane coated magnetic powder.Be put into by microspheres with solid in heat treatment furnace, pass into nitrogen protection, be raised to 600 DEG C with the temperature rise rate of 1 DEG C/min, be incubated after 1 hour, Temperature fall, obtains the microspheres with solid of porous surface.Utilize the magnetic magnetic microsphere of magnet sucking-off tool, obtain the finished product magnetic microsphere that 331g is qualified.
Obtained finished product magnetic microsphere, sodium hydroxide and amino-benzene carbonamidine are mixed with the aqueous solution with the mass ratio of 1:2.5:6, and keep pH value to be 11.2, temperature is at 80 DEG C, stirring reaction 10 hours, cooling, through magnetic resolution, washing, obtains the magnetic microsphere of surface with affinity groups.
Magnetic microsphere after activation and lsothiocyanates, Guanidinium hydrochloride, sodium iodide, EDTA, dodecylamino acid are configured to extracting solution, wherein the concentration of lsothiocyanates is 5M, the concentration of Guanidinium hydrochloride is 2.8M, the concentration of sodium iodide is 2.1M, the concentration of EDTA is 25mM, the concentration of dodecylamino acid is 75mM, and the mass concentration of magnetic microsphere is 12%.
Embodiment 4
Take Polycarbosilane solid 1000g, pulverize last, join and fill in the rotary evaporation bottle of 1000g dimethylbenzene, rotate at water-bath 50 DEG C and dissolve formation emulsion.In beaker, first add the ethylene glycol of 300g, then add bromination ten alkylammonium of 40g, after stirring, the nano-nickel powder (particle diameter is below 30nm) of 500g is joined in beaker, stirs 10 minutes, form suspension.The suspension of preparation is slowly joined in the emulsion of preparation, stirs, obtain mixed solution.In collection container, add 1000g deionized water, and add ammonia third pyridine of 100g, be heated to 80 DEG C.Above collection container, arrange microporous membrane, the aperture of microporous membrane is 40 μm.Mixed solution is added dropwise in collection container by microporous membrane.Utilize underpressure distillation means the moisture in collection container and organic solvent to be removed, obtain the microspheres with solid of Polycarbosilane coated magnetic powder.Be put into by microspheres with solid in heat treatment furnace, pass into argon shield, be raised to 600 DEG C with the temperature rise rate of 0.1 DEG C/min, be incubated after 2 hours, Temperature fall, obtains the microspheres with solid of porous surface.Utilize the magnetic magnetic microsphere of magnet sucking-off tool, obtain the finished product magnetic microsphere that 538g is qualified.
Obtained finished product magnetic microsphere, potassium hydroxide and aminophenyl boronic acid are configured to the aqueous solution with the mass ratio of 1:2:5, and keep pH value to be 11.5, temperature is at 60 DEG C, stirring reaction 8 hours, cooling, through magnetic resolution, washing, obtains the magnetic microsphere of surface with affinity groups.
Magnetic microsphere after activation and lsothiocyanates, Guanidinium hydrochloride, sodium iodide, EDTA, dodecylamino acid are configured to extracting solution, wherein the concentration of lsothiocyanates is 4.2M, the concentration of Guanidinium hydrochloride is 2.5M, the concentration of sodium iodide is 1.5M, the concentration of EDTA is 15mM, the concentration of dodecylamino acid is 100mM, and the mass concentration of magnetic microsphere is 10%.
Claims (10)
1. a preparation method for animal tissues's nucleic acid extraction liquid, comprises the following steps:
(1) preparation of magnetic microsphere
(1) by Polycarbosilane dissolution of solid in organic benzene, organic kind solvent, formed emulsion; Joined by nano-scale magnetic powder in the organic alcohols solvent containing tensio-active agent, stir formation suspension; Suspension is joined in emulsion, stir, obtain mixed solution; Wherein the mass ratio of Polycarbosilane and magnetic powder is 2-5:1;
(2) be added dropwise in the warm water solution containing linking agent by microporous membrane by mixed solution, water temperature remains on 60-80 DEG C; Make Polycarbosilane crosslinking curing in mixed solution surperficial at magnetic powder; Utilize underpressure distillation means moisture and organic solvent to be removed, obtain the microspheres with solid of Polycarbosilane coated magnetic powder;
(3) microspheres with solid is carried out insulation 1-2 hour at 500-1000 DEG C, under nitrogen or protection of inert gas, after Polycarbosilane cracking, form the microspheres with solid of porous surface;
(4) microspheres with solid after thermal treatment is screened by Magneto separate means, filter out qualified finished product magnetic microsphere;
(2) activation of magnetic microsphere
Finished product magnetic microsphere obtained in (one) is mixed with the mass ratio of 1:1-20:5-30 with catalyzer, amino agents, maintenance pH value is 10-13, temperature is at 50-80 DEG C, stirring reaction 5-10 hour, cooling, through magnetic resolution, washing, obtain the magnetic microsphere of surface with affinity groups; Wherein catalyzer is selected from sodium hydroxide or potassium hydroxide, and amino agents is selected from amino-benzene carbonamidine or aminophenyl boronic acid;
(3) preparation of extracting solution
Magnetic microsphere after activation in step (two) and lsothiocyanates, Guanidinium hydrochloride, sodium iodide, EDTA, dodecylamino acid are configured to extracting solution, wherein the concentration of lsothiocyanates is 3.5-5M, the concentration of Guanidinium hydrochloride is 1.5-3M, the concentration of sodium iodide is 1.2-2.5M, the concentration of EDTA is 15-30mM, and the concentration of dodecylamino acid is 60-100mM; The mass concentration of magnetic microsphere is 10-15%.
2. the preparation method of animal tissues according to claim 1 nucleic acid extraction liquid, in (1) of wherein step (), organic benzene, organic kind solvent is selected from the one or more combination in toluene, dimethylbenzene and Vinylstyrene.
3. the preparation method of animal tissues according to claim 1 nucleic acid extraction liquid, in (1) of wherein step (), wherein organic alcohols solvent selected from ethanol or ethylene glycol.
4. the preparation method of animal tissues according to claim 1 nucleic acid extraction liquid, in (1) of wherein step (), wherein tensio-active agent is selected from dioctyl succinate disulfonate acid, ten sodium alkyl sulfates, bromination ten alkyl trimethyl ammonium or bromination ten alkylammonium.
5. the preparation method of animal tissues according to claim 1 nucleic acid extraction liquid, in (2) of wherein step (), wherein linking agent is selected from N,N methylene bis acrylamide or ammonia third pyridine.
6. the preparation method of animal tissues according to claim 1 nucleic acid extraction liquid, in (1) of wherein step (), wherein the particle diameter of nano-scale magnetic powder is 1nm-30nm.
7. the preparation method of animal tissues according to claim 1 nucleic acid extraction liquid, in (1) of wherein step (), wherein magnetic powder is iron, cobalt, nickel or their alloy.
8. the preparation method of animal tissues according to claim 1 nucleic acid extraction liquid, in (1) of wherein step (), wherein magnetic powder is Fe
3o
4.
9. the preparation method of animal tissues according to claim 1 nucleic acid extraction liquid, in (2) of wherein step (), wherein the aperture of microporous membrane is 1-50 μm.
10. the preparation method of animal tissues according to claim 1 nucleic acid extraction liquid, in (2) of wherein step (), wherein the aperture of microporous membrane is 10-30 μm.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108531563A (en) * | 2018-02-05 | 2018-09-14 | 深圳市尚维高科有限公司 | The purposes and lysate of porous microsphere and the application method of lysate |
| CN109929831A (en) * | 2017-12-18 | 2019-06-25 | 中国科学院大连化学物理研究所 | The method of metal inner core/carbon shell structurre nano particle extraction Circulating tumor DNA |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1260595A2 (en) * | 1995-07-07 | 2002-11-27 | Toyo Boseki Kabushiki Kaisha | Nucleic acid-bondable magnetic carrier and method for isolating nucleic acid using the same |
| CN1469775A (en) * | 2000-10-18 | 2004-01-21 | 东洋钢钣株式会社 | Particulate support for separation/purification or extraction and process for producing the same |
| CN104528725A (en) * | 2015-01-08 | 2015-04-22 | 厦门大学 | Preparation method of magnetic silicon carbide ceramic nano particles |
-
2015
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1260595A2 (en) * | 1995-07-07 | 2002-11-27 | Toyo Boseki Kabushiki Kaisha | Nucleic acid-bondable magnetic carrier and method for isolating nucleic acid using the same |
| CN1469775A (en) * | 2000-10-18 | 2004-01-21 | 东洋钢钣株式会社 | Particulate support for separation/purification or extraction and process for producing the same |
| CN104528725A (en) * | 2015-01-08 | 2015-04-22 | 厦门大学 | Preparation method of magnetic silicon carbide ceramic nano particles |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109929831A (en) * | 2017-12-18 | 2019-06-25 | 中国科学院大连化学物理研究所 | The method of metal inner core/carbon shell structurre nano particle extraction Circulating tumor DNA |
| CN109929831B (en) * | 2017-12-18 | 2023-01-20 | 中国科学院大连化学物理研究所 | Method for extracting circulating tumor DNA from nanoparticles with metal core/carbon shell structure |
| CN108531563A (en) * | 2018-02-05 | 2018-09-14 | 深圳市尚维高科有限公司 | The purposes and lysate of porous microsphere and the application method of lysate |
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