CN105112200A - Preparation method of tartary buckwheat yellow koji rice wine - Google Patents

Preparation method of tartary buckwheat yellow koji rice wine Download PDF

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CN105112200A
CN105112200A CN201510420917.1A CN201510420917A CN105112200A CN 105112200 A CN105112200 A CN 105112200A CN 201510420917 A CN201510420917 A CN 201510420917A CN 105112200 A CN105112200 A CN 105112200A
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fermentation
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buckwheat
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雨田
赵钢
邹亮
郭晓恒
彭镰心
赵江林
向达兵
万燕
谭茂玲
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Chengdu University
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Abstract

The invention discloses a preparation method of tartary buckwheat yellow koji rice wine. The method comprises the following steps: inoculating Aspergillus flavus into a medium according to an inoculation amount of 0.5-2%; adding traditional Chinese medicinal material fine powder with the mass fraction of 0.5-2%, and culturing to generate mycelia; collecting a liquid carrying strain in the medium to obtain medicinal koji; and steaming a fermentation substrate, mixing the steamed substrate with the medicinal koji and yeast, naturally fermenting, squeezing, filtering, and disinfecting to obtain the tartary buckwheat yellow koji rice wine. Tartary buckwheat and other traditional Chinese medicinal materials are co-fermented under the action of Aspergillus flavus, yeast is added to ferment the fermentation substrate rice or other cereals in order to prepare the yellow rice wine with health functions, effective components of tartary buckwheat and the traditional Chinese medicinal materials are maximally reserved, and new functional components are generated through the fermentation process.

Description

The preparation method of the yellow bent wine of a kind of bitter buckwheat
Technical field
The present invention relates to wine-making technology technical field, be specifically related to a kind of preparation method of Yellow wine of buckwheat.
Background technology
Bitter buckwheat and Radix Et Rhizoma Fagopyri Tatarici, belong to dicotyledonous buckwheat, formal name used at school hull buckwheat (TartarianBuckwheat), and another name buckwheat leaf seven, wild blue buckwheat, Wan Nianqiao, spinach wheat, Wu Mai, Hua Qiao are the medicine-food two-purpose crops that occurring in nature is very few.Record according to Compendium of Material Medica: bitter buckwheat bitter, property is flat cold, the real stomach of energy, beneficial strength, continuous spirit, sharp knowledge, and refining the five internal organs slag is dirty; All on the books to bitter buckwheat in Thousand Golden Prescriptions, " Chinese medicine voluminous dictionary " and pertinent literature: can calm the nerves, wind pain is swollen in the wide intestines of lively atmosphere blood, sending down abnormally ascending, heat-clearing, long-pendingization of dispelling is stagnant, gut purge, ease constipation, defaecation, cough-relieving, relieving asthma, anti-inflammatory, antianaphylaxis, cardiac stimulant, fat-reducing, the effect such as beauty treatment.
By modern analysis method, the benefit materials in bitter buckwheat is able to be familiar with by people, its composition and effect mainly as follows:
Bitter buckwheat contains abundant flavones, tartary buckwheat flavone a kind ofly has the more complicated plyability material of diverse biological activities, and major ingredient has Quercetin, rutin, bank luxuriant and rich with fragrance alcohol, morin, and wherein rutin accounts for 80%, the ratio of this natural composition more has synergistic effect, is more easily absorbed by the body.Tartary buckwheat flavone mainly contains following several function: 1) prevention, Cardiovarscular; 2) reducing blood-fat, hypotensive, hypoglycemic activity; 3) strengthening immunity; 4) antioxygenation; 5) restraining and sterilizing bacteria, prevent brain cell aging, as anticancer agent; 6) protect gastric mucosa, repair brain injury, antidepressant; 7) promote insulin secretion, improve the avidity of insulin receptor; 8) provide protection is had to cerebral ischemia and renal ischemic damage.Bitter buckwheat contains abundant phenolic compound, i.e. pycnogenols (tartary buckwheat bran content reaches as high as 5.03%), gallic acid, Protocatechuic Acid, vanillic acid, syringic acid, forulic acid etc.Bitter buckwheat polyphenol mainly contains following several function: 1) have anti-inflammatory, anti-allergic, antiviral and anticancer characteristic; 2) having the ability of engulfing free radical, is the important sources of polyphenoils; 3) lipid oxidation can be reduced and form anti-cardiopathic protection; 4) growth of leukemia cell can be suppressed.Bitter buckwheat contains DCI, and it has positive effect to preventing and treating diabetes, has therapeutic action to polycystic ovary disease.Bitter buckwheat contains the inorganic elements calcium of multiple beneficial HUMAN HEALTH, phosphorus, iron, copper, zinc and trace element-selenium etc., the content of magnesium is more than 11 times of whole meal flour, ferro element is 2-5 times of other staple food grain, zinc is more than 1.5 times, manganese be 1.4 accompany above, potassium is 2 times of wheat, 2.3 times of rice, 1.5 times of topaz rice and flour.Selenium is wherein " anticancer king " in body trace element, has very strong antioxygenation, slows down cutin aging, anti-aging; Can interior free yl be removed, get rid of vivotoxin, prevent blood clot, remove cholesterol, strengthen immune function of human body.In addition, also containing weakly alkaline starch, activated protein, VITAMIN, useful amino acid etc. in bitter buckwheat, very strong health-care effect is had to human body.
Traditional Yellow rice wine preparation technology, its preparation process generally comprises: the step such as immersion-boiling-spreading for cooling-Jia song-fermentation-distillation-sterilization-filling.As the making method that patent discloses a kind of Radix Et Rhizoma Fagopyri Tatarici wine that application number is CN01107297.0, be that buckwheat powder is broken into buckwheat powder and Radix Et Rhizoma Fagopyri Tatarici bran, after difference blunge is steamed, obtain distillate through sealing and fermenting, distillation, then obtain Radix Et Rhizoma Fagopyri Tatarici wine through mixing, blending.Research finds, in the brewing process of Radix Et Rhizoma Fagopyri Tatarici wine, due to the fermentative action of microorganism, a part of rutin in bitter buckwheat raw material can be converted into bio-absorbable utilization ratio and higher slack skin element, the different slack skin glycosides of anti-oxidant activity.But in the distillation production process of Radix Et Rhizoma Fagopyri Tatarici wine, these functional components such as flavonoid and trace element in fermentation materials can not enter distillage, and bitter buckwheat raw material is not fully utilized.
Summary of the invention
The object of this invention is to provide a kind of preparation method of Yellow wine of buckwheat, the yellow rice wine that the method prepares not only possesses significant vessel softening, reducing blood-fat, the function that delays senility; And the resistance of oxidation of Yellow wine of buckwheat can be improved.
For reaching above-mentioned purpose, providing a kind of preparation method of Yellow wine of buckwheat in one embodiment of the present of invention, comprising the following steps:
(1) medicine song, is prepared
Accessed by Aspergillus flavus in substratum, inoculum size is 0.5% ~ 2%; Add the Chinese medicinal materials fine powder cultivation generation mycelium that massfraction is 0.5% ~ 2%; Collect the liquid with bacterial classification in substratum, obtain medicine song;
Described Chinese medicinal materials fine powder is sieved by bitter buckwheat 1 ~ 100 part, matrimony vine 1 ~ 100 part, ginseng 1 ~ 100 part, 1 ~ 100 part, shizandra berry, the tuber of dwarf lilyturf 1 ~ 100 part, the root of kudzu vine 1 ~ 100 part, hawthorn 1 ~ 100 part and pseudo-ginseng 1 ~ 100 part of co-grinding and obtains;
(2), by fermentation substrate cook, admix medicine song and yeast spontaneous fermentation, after fermentation, after squeezing, filtration, sterilizing, obtain Yellow wine of buckwheat.
As an alternative embodiment of the invention, Aspergillus flavus cultivates 24h ~ 72h under 33 DEG C ~ 37 DEG C conditions; Fermentation substrate is rice; The temperature of fermentation substrate spontaneous fermentation is 26 DEG C ~ 30 DEG C, and fermentation time is 8 days ~ 12 days; The add-on of medicine song is 5% ~ 20%; Saccharomycetic inoculum size is 0.5% ~ 2%.
In sum, the present invention has the following advantages:
The present invention is by bitter buckwheat and other Chinese medicinal materialss co-fermentation under flavus effect, then yeast and fermentation substrate rice or other fermented grains is added, the yellow rice wine with nourishing function prepared, farthest remain the effective constituent of bitter buckwheat and Chinese medicinal materials, and by fermenting process, create new functional ingredient.
Yellow wine of buckwheat of the present invention, has significant vessel softening, reducing blood-fat, anti-oxidant, the nourishing function that delays senility.Method of the present invention, whole preparation process is simple to operate, without the need to distillation, reduces production energy consumption.
Embodiment
Embodiment 1
(1) medicine song, is prepared
Accessed by Aspergillus flavus in solid medium, inoculum size is 1%; Add massfraction be 0.6% Chinese medicinal materials fine powder cultivate 2 days under 35 DEG C of conditions, when culturing bottle generates a large amount of white hypha, i.e. mycelium.Then collect the liquid with bacterial classification in substratum, obtain medicine song.
Wherein, Chinese medicinal materials fine powder is sieved by bitter buckwheat 50 parts, matrimony vine 4 parts, ginseng 8 parts, 12 parts, shizandra berry, the tuber of dwarf lilyturf 4 parts, the root of kudzu vine 20 parts, hawthorn 16 parts and pseudo-ginseng 9 parts of co-grindings and obtains.
(2), by fermentation substrate rice cook to spread out and be cooled to 28 DEG C, admix the medicine cooking rice weight 6% bent, the yeast spontaneous fermentation of access 0.6%, controlling leavening temperature is about 28 DEG C, ferments 10 days.After fermentation, fermentation substrate is carried out to squeezing water outlet, after filtration, sterilizing, obtains Yellow wine of buckwheat again.
Embodiment 2
(1) medicine song, is prepared
Accessed by Aspergillus flavus in solid medium, inoculum size is 1%; Add massfraction be 1% Chinese medicinal materials fine powder cultivate 2 days under 36 DEG C of conditions, when culturing bottle generates a large amount of white hypha, i.e. mycelium.Then collect the liquid with bacterial classification in substratum, obtain medicine song.
Wherein, Chinese medicinal materials fine powder is sieved by bitter buckwheat 80 parts, matrimony vine 16 parts, ginseng 22 parts, 28 parts, shizandra berry, the tuber of dwarf lilyturf 15 parts, the root of kudzu vine 4 parts, hawthorn 19 parts and pseudo-ginseng 20 parts of co-grindings and obtains.
(2), by fermentation substrate rice cook to spread out and be cooled to 26 DEG C, admix the medicine cooking rice weight 10% bent, the yeast spontaneous fermentation of access 0.6%, controlling leavening temperature is about 26 DEG C, ferments 12 days.After fermentation, fermentation substrate is carried out to squeezing water outlet, after filtration, sterilizing, obtains Yellow wine of buckwheat again.
Embodiment 3
(1) medicine song, is prepared
Accessed by Aspergillus flavus in solid medium, inoculum size is 1%; Add massfraction be 1.2% Chinese medicinal materials fine powder cultivate 2 days under 36 DEG C of conditions, when culturing bottle generates a large amount of white hypha, i.e. mycelium.Then collect the liquid with bacterial classification in substratum, obtain medicine song.
Wherein, Chinese medicinal materials fine powder is sieved by bitter buckwheat 100 parts, matrimony vine 20 parts, ginseng 30 parts, 12 parts, shizandra berry, the tuber of dwarf lilyturf 4 parts, the root of kudzu vine 14 parts, hawthorn 9 parts and pseudo-ginseng 10 parts of co-grindings and obtains.
(2), by fermentation substrate rice cook to spread out and be cooled to 26 DEG C, admix the medicine cooking rice weight 10% bent, the yeast spontaneous fermentation of access 1%, controlling leavening temperature is about 26 DEG C, ferments 12 days.After fermentation, fermentation substrate is carried out to squeezing water outlet, after filtration, sterilizing, obtains Yellow wine of buckwheat again.
Embodiment 4
Do not add the making method of the Chinese medicinal materials fine powder containing bitter buckwheat
Cooked to spread out by fermentation substrate rice and be cooled to 28 DEG C, the yeast spontaneous fermentation of access 0.6%, controlling leavening temperature is about 28 DEG C, ferments 10 days.After fermentation, fermentation substrate is carried out to squeezing water outlet, after filtration, sterilizing, obtains Yellow wine of buckwheat again.
Different Chinese medicinal materials fine powder component is on the impact of the resistance of oxidation in yellow rice wine product:
Embodiment 5
(1) medicine song, is prepared
Accessed by Aspergillus flavus in solid medium, inoculum size is 1%; Add massfraction be 0.6% Chinese medicinal materials fine powder cultivate 2 days under 35 DEG C of conditions, when culturing bottle generates a large amount of white hypha, i.e. mycelium.Then collect the liquid with bacterial classification in substratum, obtain medicine song.
Wherein, Chinese medicinal materials fine powder is sieved obtained by bitter buckwheat 50 parts, matrimony vine 4 parts of co-grinding.
(2), by fermentation substrate rice cook to spread out and be cooled to 28 DEG C, admix the medicine cooking rice weight 6% bent, the yeast spontaneous fermentation of access 0.6%, controlling leavening temperature is about 28 DEG C, ferments 10 days.After fermentation, fermentation substrate is carried out to squeezing water outlet, after filtration, sterilizing, obtains Yellow wine of buckwheat again.
Embodiment 6
(1) medicine song, is prepared
Accessed by Aspergillus flavus in solid medium, inoculum size is 1%; Add massfraction be 0.6% Chinese medicinal materials fine powder cultivate 2 days under 35 DEG C of conditions, when culturing bottle generates a large amount of white hypha, i.e. mycelium.Then collect the liquid with bacterial classification in substratum, obtain medicine song.
Wherein, Chinese medicinal materials fine powder is sieved by ginseng 8 parts, 12 parts, shizandra berry, the tuber of dwarf lilyturf 4 parts, the root of kudzu vine 20 parts, hawthorn 16 parts and pseudo-ginseng 9 parts of co-grindings and obtains.
(2), by fermentation substrate rice cook to spread out and be cooled to 28 DEG C, admix the medicine cooking rice weight 6% bent, the yeast spontaneous fermentation of access 0.6%, controlling leavening temperature is about 28 DEG C, ferments 10 days.After fermentation, fermentation substrate is carried out to squeezing water outlet, after filtration, sterilizing, obtains Yellow wine of buckwheat again.
Embodiment 7
(1) medicine song, is prepared
Accessed by Aspergillus flavus in solid medium, inoculum size is 1%; Add massfraction be 0.6% Chinese medicinal materials fine powder cultivate 2 days under 35 DEG C of conditions, when culturing bottle generates a large amount of white hypha, i.e. mycelium.Then collect the liquid with bacterial classification in substratum, obtain medicine song.
Wherein, Chinese medicinal materials fine powder is sieved by bitter buckwheat 50 parts, matrimony vine 4 parts, the tuber of dwarf lilyturf 4 parts, the root of kudzu vine 20 parts, hawthorn 16 parts and pseudo-ginseng 9 parts of co-grindings and obtains.
(2), by fermentation substrate rice cook to spread out and be cooled to 28 DEG C, admix the medicine cooking rice weight 6% bent, the yeast spontaneous fermentation of access 0.6%, controlling leavening temperature is about 28 DEG C, ferments 10 days.After fermentation, fermentation substrate is carried out to squeezing water outlet, after filtration, sterilizing, obtains Yellow wine of buckwheat again.
Embodiment 8
(1) medicine song, is prepared
Accessed by Aspergillus flavus in solid medium, inoculum size is 1%; Add massfraction be 0.6% Chinese medicinal materials fine powder cultivate 2 days under 35 DEG C of conditions, when culturing bottle generates a large amount of white hypha, i.e. mycelium.Then collect the liquid with bacterial classification in substratum, obtain medicine song.
Wherein, Chinese medicinal materials fine powder by bitter buckwheat 50 parts, matrimony vine 4 parts, ginseng 8 parts, 12 parts, shizandra berry, the tuber of dwarf lilyturf 4 parts of co-grindings sieve and obtain.
(2), by fermentation substrate rice cook to spread out and be cooled to 28 DEG C, admix the medicine cooking rice weight 6% bent, the yeast spontaneous fermentation of access 0.6%, controlling leavening temperature is about 28 DEG C, ferments 10 days.After fermentation, fermentation substrate is carried out to squeezing water outlet, after filtration, sterilizing, obtains Yellow wine of buckwheat again.
Different strain is on the impact of yellow rice wine resistance of oxidation
Embodiment 9
(1) medicine song, is prepared
Accessed by black-koji mould in solid medium, inoculum size is 1%; Add massfraction be 0.6% Chinese medicinal materials fine powder cultivate 2 days under 35 DEG C of conditions, when culturing bottle generates a large amount of white hypha, i.e. mycelium.Then collect the liquid with bacterial classification in substratum, obtain medicine song.
Wherein, Chinese medicinal materials fine powder is sieved by bitter buckwheat 50 parts, matrimony vine 4 parts, ginseng 8 parts, 12 parts, shizandra berry, the tuber of dwarf lilyturf 4 parts, the root of kudzu vine 20 parts, hawthorn 16 parts and pseudo-ginseng 9 parts of co-grindings and obtains.
(2), by fermentation substrate rice cook to spread out and be cooled to 28 DEG C, admix the medicine cooking rice weight 6% bent, the yeast spontaneous fermentation of access 0.6%, controlling leavening temperature is about 28 DEG C, ferments 10 days.After fermentation, fermentation substrate is carried out to squeezing water outlet, after filtration, sterilizing, obtains Yellow wine of buckwheat again.
Embodiment 10
(1) medicine song, is prepared
Accessed by monascus ruber in solid medium, inoculum size is 1%; Add massfraction be 0.6% Chinese medicinal materials fine powder cultivate 2 days under 35 DEG C of conditions, when culturing bottle generates a large amount of white hypha, i.e. mycelium.Then collect the liquid with bacterial classification in substratum, obtain medicine song.
Wherein, Chinese medicinal materials fine powder is sieved by bitter buckwheat 50 parts, matrimony vine 4 parts, ginseng 8 parts, 12 parts, shizandra berry, the tuber of dwarf lilyturf 4 parts, the root of kudzu vine 20 parts, hawthorn 16 parts and pseudo-ginseng 9 parts of co-grindings and obtains.
(2), by fermentation substrate rice cook to spread out and be cooled to 28 DEG C, admix the medicine cooking rice weight 6% bent, the yeast spontaneous fermentation of access 0.6%, controlling leavening temperature is about 28 DEG C, ferments 10 days.After fermentation, fermentation substrate is carried out to squeezing water outlet, after filtration, sterilizing, obtains Yellow wine of buckwheat again.
Embodiment 11
(1) medicine song, is prepared
Accessed by yeast in solid medium, inoculum size is 1%; Add massfraction be 0.6% Chinese medicinal materials fine powder cultivate 2 days under 35 DEG C of conditions, when culturing bottle generates a large amount of white hypha, i.e. mycelium.Then collect the liquid with bacterial classification in substratum, obtain medicine song.
Wherein, Chinese medicinal materials fine powder is sieved by bitter buckwheat 50 parts, matrimony vine 4 parts, ginseng 8 parts, 12 parts, shizandra berry, the tuber of dwarf lilyturf 4 parts, the root of kudzu vine 20 parts, hawthorn 16 parts and pseudo-ginseng 9 parts of co-grindings and obtains.
(2), by fermentation substrate rice cook to spread out and be cooled to 28 DEG C, admix the medicine cooking rice weight 6% bent, the yeast spontaneous fermentation of access 0.6%, controlling leavening temperature is about 28 DEG C, ferments 10 days.After fermentation, fermentation substrate is carried out to squeezing water outlet, after filtration, sterilizing, obtains Yellow wine of buckwheat again.
Detect the yellow rice wine prepared according to method in embodiment 1 ~ embodiment 11, use DPPH method to measure its elimination factor to free radical, namely can judge the resistance of oxidation of yellow rice wine, result is as table 1.
Table 1: the free radical scavenging activity of each embodiment yellow rice wine
Embodiment Clearance rate Embodiment Clearance rate
Embodiment 1 46.2% Embodiment 7 41.2%
Embodiment 2 49.8% Embodiment 8 40.6%
Embodiment 3 52.6% Embodiment 9 28.6%
Embodiment 4 1.2% Embodiment 10 29.4%
Embodiment 5 32.5% Embodiment 11 12.3%
Embodiment 6 39.8%
Interpretation of result:
Can learn from the experimental result of above-mentioned table 1, the Yellow wine of buckwheat in embodiment 1 ~ embodiment 3 has good resistance of oxidation.The Chinese medicine powder such as bitter buckwheat are not added in embodiment 4, its resistance of oxidation is extremely low, if when getting rid of experimental result error, its actual resistance of oxidation is still very low, illustrate in the preparation process of yellow rice wine, herb fermenting product containing bitter buckwheat has tremendous influence to antioxygenation, can significantly improve the resistance of oxidation of yellow rice wine.
Can learn from the experimental result of embodiment 5 ~ embodiment 8, on the decrease after medicinal material component, the resistance of oxidation of yellow rice wine reduces, the highest in the embodiment 1 containing whole Chinese medicinal materials, illustrate that other components of Chinese medicinal materials in present method have the effect of synergy to the antioxidant property in bitter buckwheat and yellow rice wine, be because other medicinal materials except bitter buckwheat create antioxidant in fermentation, enhance the metabolic capacity of antioxidant total amount.
Can learn from the experimental result of embodiment 9 ~ embodiment 11, use metabolism antioxidant ability during other bacterial classifications to reduce.
In sum, method of the present invention, the first has highly active Quercetin and isoquercitrin raising resistance of oxidation by improving rutin conversion generation in bitter buckwheat; And by suitable traditional Chinese medicinal components, make other components in the raising that jointly can promote resistance of oxidation by fermentation later.

Claims (6)

1. a preparation method for Yellow wine of buckwheat, comprises the following steps:
(1) medicine song, is prepared
Accessed by Aspergillus flavus in substratum, inoculum size is 0.5% ~ 2%; Add the Chinese medicinal materials fine powder cultivation generation mycelium that massfraction is 0.5% ~ 2%; Collect the liquid with bacterial classification in substratum, obtain medicine song;
Described Chinese medicinal materials fine powder is sieved by bitter buckwheat 1 ~ 100 part, matrimony vine 1 ~ 100 part, ginseng 1 ~ 100 part, 1 ~ 100 part, shizandra berry, the tuber of dwarf lilyturf 1 ~ 100 part, the root of kudzu vine 1 ~ 100 part, hawthorn 1 ~ 100 part and pseudo-ginseng 1 ~ 100 part of co-grinding and obtains;
(2), by fermentation substrate cook, admix medicine song and yeast spontaneous fermentation, after fermentation, after squeezing, filtration, sterilizing, obtain Yellow wine of buckwheat.
2. the method for claim 1, is characterized in that: described Aspergillus flavus cultivates 24h ~ 72h under 33 DEG C ~ 37 DEG C conditions.
3. the method for claim 1, is characterized in that: described fermentation substrate is rice.
4. the method for claim 1, is characterized in that: the temperature of described fermentation substrate spontaneous fermentation is 26 DEG C ~ 30 DEG C, and fermentation time is 8 days ~ 12 days.
5. the method for claim 1, is characterized in that: the add-on of described medicine song is 5% ~ 20%.
6. the method for claim 1, is characterized in that: described saccharomycetic inoculum size is 0.5% ~ 2%.
CN201510420917.1A 2015-07-17 2015-07-17 Preparation method of tartary buckwheat yellow koji rice wine Pending CN105112200A (en)

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CN106047560A (en) * 2015-07-17 2016-10-26 成都大学 Preparation method of tartary buckwheat rice wine
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CN106047560A (en) * 2015-07-17 2016-10-26 成都大学 Preparation method of tartary buckwheat rice wine
CN105907520A (en) * 2016-05-31 2016-08-31 河南茗轩食品科技有限公司 Herbal medicine starter for yellow rice wine, Chinese wolfberry eucommia yellow rice wine and preparation method thereof
CN106591033A (en) * 2016-11-30 2017-04-26 绍兴国家黄酒工程技术研究中心有限公司 Production process of tartary buckwheat yellow wine with Shaoxing yellow wine flavor
CN107058025A (en) * 2017-05-27 2017-08-18 湖北楚园春酒业有限公司 A kind of trophism bitter buckwheat wine and its production method
CN107603811A (en) * 2017-10-27 2018-01-19 湖北工业大学 A kind of brewing method of bitter buckwheat wine

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