CN105112160A - Preparation method for beet flavor for tobacco - Google Patents
Preparation method for beet flavor for tobacco Download PDFInfo
- Publication number
- CN105112160A CN105112160A CN201510462750.5A CN201510462750A CN105112160A CN 105112160 A CN105112160 A CN 105112160A CN 201510462750 A CN201510462750 A CN 201510462750A CN 105112160 A CN105112160 A CN 105112160A
- Authority
- CN
- China
- Prior art keywords
- beet
- spices
- preparation
- cellulase
- cigarette
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000335053 Beta vulgaris Species 0.000 title claims abstract description 44
- 235000016068 Berberis vulgaris Nutrition 0.000 title claims abstract description 43
- 238000002360 preparation method Methods 0.000 title claims abstract description 22
- 241000208125 Nicotiana Species 0.000 title abstract description 8
- 235000002637 Nicotiana tabacum Nutrition 0.000 title abstract description 8
- 239000000796 flavoring agent Substances 0.000 title abstract description 6
- 235000019634 flavors Nutrition 0.000 title abstract description 4
- 235000019504 cigarettes Nutrition 0.000 claims abstract description 73
- 238000000034 method Methods 0.000 claims abstract description 34
- 108010059892 Cellulase Proteins 0.000 claims abstract description 33
- 229940106157 cellulase Drugs 0.000 claims abstract description 33
- 239000007788 liquid Substances 0.000 claims abstract description 26
- 238000004062 sedimentation Methods 0.000 claims abstract description 23
- 238000001914 filtration Methods 0.000 claims abstract description 17
- 108090000790 Enzymes Proteins 0.000 claims abstract description 14
- 102000004190 Enzymes Human genes 0.000 claims abstract description 14
- 229940088598 enzyme Drugs 0.000 claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 13
- 238000006243 chemical reaction Methods 0.000 claims abstract description 10
- 239000012153 distilled water Substances 0.000 claims abstract description 7
- 239000000706 filtrate Substances 0.000 claims abstract description 7
- 239000002002 slurry Substances 0.000 claims abstract description 7
- 238000010009 beating Methods 0.000 claims abstract description 4
- 241000219310 Beta vulgaris subsp. vulgaris Species 0.000 claims description 80
- 235000013599 spices Nutrition 0.000 claims description 74
- 235000021537 Beetroot Nutrition 0.000 claims description 49
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 33
- 235000021536 Sugar beet Nutrition 0.000 claims description 31
- 230000008569 process Effects 0.000 claims description 13
- 238000003756 stirring Methods 0.000 claims description 11
- 238000012545 processing Methods 0.000 claims description 6
- 239000012528 membrane Substances 0.000 claims description 5
- 108091006091 regulatory enzymes Proteins 0.000 claims description 5
- 230000008719 thickening Effects 0.000 claims description 5
- 238000005352 clarification Methods 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims description 2
- 229920002678 cellulose Polymers 0.000 abstract description 54
- 239000001913 cellulose Substances 0.000 abstract description 52
- 239000000779 smoke Substances 0.000 abstract description 5
- 235000015191 beet juice Nutrition 0.000 abstract 2
- 239000002085 irritant Substances 0.000 abstract 1
- 231100000021 irritant Toxicity 0.000 abstract 1
- 235000010980 cellulose Nutrition 0.000 description 51
- 239000000243 solution Substances 0.000 description 18
- UGFAIRIUMAVXCW-UHFFFAOYSA-N Carbon monoxide Chemical compound [O+]#[C-] UGFAIRIUMAVXCW-UHFFFAOYSA-N 0.000 description 11
- 239000003546 flue gas Substances 0.000 description 11
- 230000000694 effects Effects 0.000 description 8
- 238000012360 testing method Methods 0.000 description 7
- 238000002834 transmittance Methods 0.000 description 7
- 239000000203 mixture Substances 0.000 description 6
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 239000002304 perfume Substances 0.000 description 5
- 238000001556 precipitation Methods 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 230000000638 stimulation Effects 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 150000001720 carbohydrates Chemical class 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000009472 formulation Methods 0.000 description 4
- 235000019633 pungent taste Nutrition 0.000 description 4
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 3
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 239000008103 glucose Substances 0.000 description 3
- 230000000391 smoking effect Effects 0.000 description 3
- 108010001682 Dextranase Proteins 0.000 description 2
- 238000004380 ashing Methods 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 235000013355 food flavoring agent Nutrition 0.000 description 2
- 239000003205 fragrance Substances 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 238000010561 standard procedure Methods 0.000 description 2
- 235000000346 sugar Nutrition 0.000 description 2
- 235000021533 Beta vulgaris Nutrition 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 101000925662 Enterobacteria phage PRD1 Endolysin Proteins 0.000 description 1
- 229920002488 Hemicellulose Polymers 0.000 description 1
- 102000002568 Multienzyme Complexes Human genes 0.000 description 1
- 108010093369 Multienzyme Complexes Proteins 0.000 description 1
- KWYUFKZDYYNOTN-UHFFFAOYSA-M Potassium hydroxide Chemical compound [OH-].[K+] KWYUFKZDYYNOTN-UHFFFAOYSA-M 0.000 description 1
- 240000000111 Saccharum officinarum Species 0.000 description 1
- 235000007201 Saccharum officinarum Nutrition 0.000 description 1
- 229930006000 Sucrose Natural products 0.000 description 1
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000002547 anomalous effect Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 229910052785 arsenic Inorganic materials 0.000 description 1
- RQNWIZPPADIBDY-UHFFFAOYSA-N arsenic atom Chemical compound [As] RQNWIZPPADIBDY-UHFFFAOYSA-N 0.000 description 1
- 239000010425 asbestos Substances 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 108010047754 beta-Glucosidase Proteins 0.000 description 1
- 102000006995 beta-Glucosidase Human genes 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000001461 cytolytic effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 229960004132 diethyl ether Drugs 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 235000021552 granulated sugar Nutrition 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 230000000704 physical effect Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 229910052895 riebeckite Inorganic materials 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 238000000967 suction filtration Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000002522 swelling effect Effects 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Landscapes
- Seasonings (AREA)
Abstract
The invention provides a preparation method for a beet flavor for tobacco. The preparation method comprises the following steps: (1) beating beet to collect slurry so as to obtain beet juice; (2) adding distilled water into cellulase to dissolve the cellulase, filtering the solution and fetching the filtrate to obtain a cellulase solution, and adding the cellulase solution into the beet juice for enzyme treatment reaction to obtain an enzyme treatment solution; (3) adjusting the pH of the enzyme treatment solution to a cellulase isoelectric point for isoelectric point sedimentation, and allowing the solution to stand to obtain an enzyme treatment sedimentation liquid; (4) coarsely filtering and finely filtering the enzyme treatment sedimentation liquid to obtain a clear sedimentation liquid; and (5) carrying out vacuum concentration on the clear sedimentation liquid to obtain the beet flavor. The method provided by the invention can be used for removing a lot of cellulose in beet, and can be used for improving irritant taste caused by a lot of cellulose if being applied to cigarettes, so that the smoke is more fine, smooth and flexible. The method is relatively high in cellulose reducing rate and simple in preparation process.
Description
Technical field
The present invention relates to essence spice for cigarette technical field, refer to a kind of preparation method of cigarette garden beet spices particularly.
Background technology
Beet (Betavulgaris), You Ming Chard dish, biennial herb plant, root is coniform to fusiform, succulence.Moisture accounts for 75%, and solid substance accounts for 25%.In solid substance, sucrose accounts for 16% ~ 18%, and nonsugar accounts for 7% ~ 9%.Nonsugar is divided into again solubility and insoluble two kinds: insoluble non-saccharide is Mierocrystalline cellulose, hemicellulose, protofibril quality and protein mainly; Solubility non-saccharide is divided into again inorganic non-saccharide and organic non-saccharide.Beet tails is the main source of edible granulated sugar, and world today's beet cultivated area accounts for 48% of sugar crop, is only second to sugarcane and occupies the 2nd.The glucide that the block root of beet is rich in because of it, is with a wide range of applications in essence and flavoring agent industry.Be that cigarette perfume material prepared by raw material with beet, there is the effect of soft fine and smooth flue gas in cigarette.But, because of a large amount of Mierocrystalline celluloses wherein contained, pungency and residual counter productive can be brought to cigarette.Therefore, the portion of cellulose material removed in beet effectively can improve the quality of cigarette garden beet spices.In prior art, in removing beet, cellulosic method has the modes such as sinking agent precipitation, alcohol precipitation, but these processing modes are unfavorable for keeping the characteristic aroma component in spices, and complicated operation, and cost increases, and Mierocrystalline cellulose reduced rate is not high.
Summary of the invention
The object of the invention is to solve above shortcomings in prior art, a kind of preparation method of cigarette garden beet spices is provided, the method can be degraded and be removed a large amount of Mierocrystalline cellulose in beet, thus beet spices is applied in cigarette as essence and flavoring agent, the pungency mouthfeel that a large amount of Mierocrystalline cellulose brings can be improved, make flue gas finer and smoother soft, the method Mierocrystalline cellulose reduced rate is higher, and preparation technology is simple.
The preparation method of a kind of cigarette garden beet spices of the present invention, comprises the following steps:
Step 1: beet is carried out making beating process, collect slurries, described slurries are sugar beet juice;
Step 2: add distilled water and dissolve in cellulase, after filtering, get filtrate, obtain cellulase solution; Described cellulase solution is added in the sugar beet juice of step 1, carries out ferment treatment reaction, obtain ferment treatment liquid;
Step 3: in regulating step 2, the pH of ferment treatment liquid is to iso-electric point, carries out iso-electric point sedimentation, leaves standstill, obtains ferment treatment sedimentation liquid;
Step 4: ferment treatment sedimentation liquid in step 3 is first carried out coarse filtration, then coarse filtration liquid is carried out essence filter, obtain the sedimentation liquid clarified;
Step 5: the sedimentation liquid of clarification in step 4 is carried out concentrating under reduced pressure, obtains beet spices.
Further, in described step 2, the distilled water added in cellulase and the mass ratio of cellulase are 5:1, after filtering, get filtrate, obtain cellulase solution, when carrying out ferment treatment reaction, the amount ratio of cellulase solution and sugar beet juice is 0.1 ~ 10 gram: 1 liter.
Further, in described step 2, ferment treatment reaction pH value be 3 ~ 7, ferment treatment temperature carries out at being 25 ~ 40 DEG C, enzyme processing time is 5 ~ 30 hours.
Further, in described step 2,0.8 micron membrane filter is adopted to filter.
Further, in described step 3, the pH value of regulatory enzyme treatment solution is 8 ~ 10.
Further, in described step 3, constantly stir in iso-electric point settling process, described mixing speed is 6000 ~ 12000 revs/min, and churning time is 10 ~ 30 minutes.
Further, in described step 3, the time left standstill is no less than 12 hours.
Further, in described step 4, adopt gauze to carry out coarse filtration, carry out essence filter with 0.8 micron membrane filter.
Further, in the concentration process in described step 5, thickening temperature is 50 ~ 80 DEG C, and concentrated pressure is 0.01 ~ 0.2 normal atmosphere.
Further, the relative density being concentrated into beet spices is 1.100 ~ 1.250g/cm
3.
The cellulase that the present invention adopts is an a kind of cellulolytic multienzyme complex, primarily of endo-type dextranase, and circumscribed-type dextranase and cellobiase three kinds of lytic enzyme compositions.Cellulosic hydrolysis is exactly under these three kinds of enzyme synergies, cellulosic materials is decomposed completely.Natural cellulosic materials, under the effect of protofibril element enzyme, is finally hydrolyzed to water dissolvable glucose and polysaccharose substance.Therefore, utilize cellulose treatment sugar beet juice, the Mierocrystalline cellulose that can not only effectively degrade in juice, the distinctive aroma component of good maintenance beet, meanwhile, the water dissolvable glucose after cellulose degradation and polysaccharose substance have soft flue gas in cigarette, improve the effect of mouthfeel and humectation.
Consider that cellulase itself is a kind of protein, excessive enzyme to exist in spices and residual, albumen can be brought to mix the negative impacts such as gas to cigarette.Therefore the present invention is after using the Mierocrystalline cellulose in cellulose degraded beet, adopts iso-electric point settling process, and removing cellulase, obtains quality good cigarette garden beet spices.
When iso-electric point, protein molecule exists with zwitterionic form, its molecule net charge is zero (namely positive and negative charge is equal), now protein molecule particle is not in the solution because having the mutual repulsion of identical charges, molecule reaction force attenuation each other, its particle very easily collides, condense and produce precipitation, so protein is when iso-electric point, its solubleness is minimum, the most easily forms throw out.Many physical propertiess during iso-electric point such as viscosity, swelling property, osmotic pressure etc. all diminish, thus are conducive to the filtration of suspension.
The present invention is with cellulose treatment sugar beet juice, and its content of cellulose of degrading, will increasing cigarette pungency and residual cellulose conversion for having soft flue gas, improving water dissolvable glucose and the polysaccharose substance of mouthfeel and humectation effect; Adopt iso-electric point settling process again, removing cellulase, thus obtain quality good characteristic cigarette garden beet spices.
Beneficial effect of the present invention is mainly manifested in: the cigarette garden beet spices prepared by the present invention, owing to eliminating Mierocrystalline celluloses a large amount of in beet by cellulose treatment, content of cellulose reduces, the content of reducing sugar increases, simultaneously, adopt the enzyme that iso-electric point settling process removing ferment treatment uses, add in cigarette, there is reduction pungency, soft flue gas, improve mouthfeel and humectation effect, compared with not using the beet spices of cellulose treatment, beet spices prepared by the present invention is lower at cigarette moderate stimulation, residual less; Sugar beet juice is after cellulose treatment, and its viscosity reduces, thus decreases the filtrate loss in sugar beet juice filtration, adds the yield of sugar beet juice; The method Mierocrystalline cellulose reduced rate is high, and reduced rate reaches more than 82%, and preparation method is simple, and convenient operation and automatization, easily produce conversion, and cost is comparatively cheap.
Embodiment
Below in conjunction with specific embodiment, the present invention is further illustrated:
Embodiment 1
Get 500 grams of fresh beets, add 2.5L clean water twice, with juice extractor by the beet making beating process after cleaning, collect slurries, described slurries are sugar beet juice; Get 5g cellulase (this cellulase is bought in Hangzhou Zhong Xiang Chemical Co., Ltd.), add the distilled water stirring and dissolving of 25mL quality, then use the membrane filtration of 0.8 micron, get filtrate, obtain cellulase solution; Getting 0.1g cellulase solution adds in 1L sugar beet juice, pH value be 3, temperature carries out ferment treatment at being 25 DEG C and reacts 5 hours, obtains ferment treatment liquid; Again to adjust the pH value of ferment treatment liquid under the stirring velocity of 6000 revs/min for 8, carry out iso-electric point sedimentation, stir 30 minutes, leave standstill 24 hours, obtain ferment treatment sedimentation liquid; By ferment treatment sedimentation liquid gauze coarse filtration, filter out large granular impurity, then coarse filtration liquid is filtered again with the filter membrane of 0.8 micron carry out essence filter, obtain the sedimentation liquid clarified; By the sedimentation liquid of clarification temperature be 50 DEG C, pressure carries out concentrating under reduced pressure under being 0.01 normal atmosphere, the relative density being concentrated into beet spices is 1.100g/cm
3.The sugar beet juice of squeezing compared by cigarette garden beet spices prepared by the inventive method, and cellulose concentration reduces 82.53%, and compare without the identical cigarette garden beet spices of other treatment steps of cellulose treatment, cellulose concentration reduces 27.84%.
Embodiment 2
Repeat embodiment 1 by described same steps, the amount ratio unlike cellulase solution used and sugar beet juice is 1 gram of cellulase solution: 1 liter of sugar beet juice; The pH value of ferment treatment is 5, and ferment treatment temperature is 30 DEG C, and enzyme processing time is 12 hours; When carrying out iso-electric point sedimentation, stirring velocity is 7000 revs/min, and the pH value of regulatory enzyme treatment solution is 9, stirs 30 minutes, leaves standstill 12 hours; In concentration process, thickening temperature is 60 DEG C, and concentrated pressure is 0.1 normal atmosphere.The relative density being concentrated into beet spices is 1.157g/cm
3.The sugar beet juice of squeezing compared by cigarette garden beet spices prepared by the inventive method, and cellulose concentration reduces 84.50%, and compare without the identical cigarette garden beet spices of other treatment steps of cellulose treatment, cellulose concentration reduces 30.24%.
Embodiment 3
Repeat embodiment 1 by described same steps, the amount ratio unlike cellulase solution used and sugar beet juice is 5 grams of cellulase solution: 1 liter of sugar beet juice; The pH value of ferment treatment is 5, and ferment treatment temperature is 40 DEG C, and enzyme processing time is 15 hours; When carrying out iso-electric point sedimentation, stirring velocity is 7000 revs/min, and the pH value of regulatory enzyme treatment solution is 9, stirs 30 minutes, leaves standstill 14 hours; In concentration process, thickening temperature is 60 DEG C, and concentrated pressure is 0.1 normal atmosphere.The relative density being concentrated into beet spices is 1.165g/cm
3.The sugar beet juice of squeezing compared by cigarette garden beet spices prepared by the inventive method, and cellulose concentration reduces 86.50%, and compare without the identical cigarette garden beet spices of other treatment steps of cellulose treatment, cellulose concentration reduces 31.56%.
Embodiment 4
Repeat embodiment 1 by described same steps, the amount ratio unlike cellulase solution used and sugar beet juice is 10 grams of cellulase solution: 1 liter of sugar beet juice; The pH value of ferment treatment is 7, and ferment treatment temperature is 40 DEG C, and enzyme processing time is 30 hours; When carrying out iso-electric point sedimentation, stirring velocity is 12000 revs/min, and the pH value of regulatory enzyme treatment solution is 10, stirs 10 minutes, leaves standstill 18 hours; In concentration process, thickening temperature is 80 DEG C, and concentrated pressure is 0.2 normal atmosphere.The relative density being concentrated into beet spices is 1.250g/cm
3.The sugar beet juice of squeezing compared by cigarette garden beet spices prepared by the inventive method, and cellulose concentration reduces 88.60%, and compare without the identical cigarette garden beet spices of other treatment steps of cellulose treatment, cellulose concentration reduces 32.40%.
The applied research of beet spices in cigarette:
Below all for the beet spices prepared by embodiment 1, in the application of cigarette, carry out research explanation.
1, the physical index of beet spices detects:
The physical index of the beet spices obtained by above-mentioned preparation technology is as follows:
| Title | Cigarette garden beet spices |
| Outward appearance | Clarification brown liquid |
| Fragrance | Fragrant and sweet |
| Relative density (20/20 DEG C) | 1.1015 |
| Refractive index (20 DEG C) | 1.4528 |
| Miscible degree (25 DEG C) in ethanol | This extract of 1 volume dissolves in 1-20 v aqueous solution |
| Lead content (mg/kg) | ≤5.0 |
| Arsenic content (mg/kg) | ≤1.0 |
2, the application of beet spices in cigarette prepared of the present invention: have two purposes, one is that direct perfuming uses, and two is use in perfume formulation.For these two aspects, select high, medium and low pipe tobacco to carry out reinforced experiment in perfuming experiment and application to perfume formulation, and smoke panel test.
Concrete outcome is as follows:
Perfuming is tested: the concentration gradient of configuration beet spices, that is: account for pipe tobacco weight 0.02%, 0.1%, 0.5%, 0.8%, 1.0% (weight ratio to pipe tobacco) by extract respectively and join (solvent is water) in the high, medium and low pipe tobacco of 200 grams respectively.After sealed plastic bag sealing, put 50 DEG C, baking oven and dry after 30 minutes, temperature 22 ± 1 DEG C, humidity is balance 2 hours under 60 ± 1% environment, then rolls into cigarette, does contrast smoke panel test with the blank cigarette not adding beet spices.
Be applied to reinforced experiment in perfume formulation: 10% essence 1028 propylene glycol solution 10 parts and 90 parts beet spices mixing, the change of its physical behavior of continuous observation, without ANOMALOUS VARIATIONS such as precipitation, precipitations in 14 days.Get certain density mixed solution, 80 DEG C of sealings after 30 minutes, temperature 22 ± 1 DEG C, humidity is balance 2 hours under 60 ± 1% environment, then rolls into cigarette, does contrast smoke panel test with essence 1028 at the blank cigarette of different class.
7 people's compositions are smoked panel test group, and wherein national level is smoked panel test Shi Yiming, provincial teacher of the smokeing panel test two.Smoking result shows, beet spices has reduction stimulation, soft flue gas, improves mouthfeel and humectation effect, can use as Novel tobacco flavor.
Smoking result is as follows:
1. Load unload response ratio:
2. reinforced experiment in perfume formulation is applied to:
Smoking result shows that beet spices and other essence compatiblenesies are good, is added in cigarette to have to have to reduce stimulation, soft flue gas, improves mouthfeel and humectation effect, and is applicable in the cigarette of different class.
3, according to above-mentioned evaluation method of smokeing panel test equally, the sugar beet juice of squeezing, the cigarette garden beet spices prepared without cigarette garden beet spices and the inventive method of cellulose treatment are contrasted.Cigarette garden beet spices without cellulose treatment is compared with the inventive method, and only lacked cellulose treatment step, other are identical.The results are shown in following table.
| Sample ID | Cigarette evaluation |
| The sugar beet juice of squeezing | Reduce stimulation, soft flue gas, lingual surface has residual |
| Without the cigarette garden beet spices of cellulose treatment | Reduce and stimulate, soft flue gas, slightly residual |
| Cigarette garden beet spices prepared by the inventive method | Reduce stimulation, soft flue gas, improve mouthfeel and humectation |
Evaluation and analysis result shows, in the application of cigarette, the flue gas mouthfeel that the cigarette garden beet spices of the preparation identical with the present invention of other steps without cellulose treatment brings than the sugar beet juice directly squeezed is better, and cigarette garden beet spices prepared by the present invention is more obvious than the improvement of cigarette garden beet spices to cigarette smoke and mouthfeel prepared without cellulose treatment.
4, the contrast of cigarette garden beet spices transmittance:
The sugar beet juice of squeezing, the cigarette garden beet spices prepared without cigarette garden beet spices and the inventive method of cellulose treatment are carried out the detection of transmittance.Cigarette garden beet spices without cellulose treatment is compared with the inventive method, and only lacked cellulose treatment step, other are identical.Get the tobacco aromatics using after different treatment, take distilled water as contrast, under 650nm, measure transmittance.Comparing result is listed in the table below.From data in table, transmittance is higher, and clarity is more good limpider, more not free settling between preservation period; Transmittance is lower, and clarity is more poor more muddy, gets over free settling between preservation period.The transmittance of cigarette garden beet spices prepared by the present invention is minimum, shows that this beet spices not easily residues in cigarette.
| Sample ID | Transmittance (%T) |
| The sugar beet juice of squeezing | 95.0 |
| Without the cigarette garden beet spices of cellulose treatment | 93.3 |
| Cigarette garden beet spices prepared by the inventive method | 81.4 |
5, the contrast of cigarette garden beet spices Mierocrystalline cellulose reduced rate
Mierocrystalline cellulose detects: carry out cellulosic detection according to national standard method " in food coarse-fibred measuring method ".
The sugar beet juice of squeezing, the cigarette garden beet spices prepared without cigarette garden beet spices and the inventive method of cellulose treatment are carried out the detection of Mierocrystalline cellulose reduced rate.Take 20 ~ 30g beet fragrance sample, use 1.25% vitriolization, then use 1.25% potassium hydroxide solution, move into the dry G2 weighed and hang down and melt crucible or the vertical of same model is melted in funnel, suction filtration, after fully washing with hot water, drain.Use ethanol and washed with diethylether successively once again.Weigh after crucible and content being dried in 105 DEG C of baking ovens, repetitive operation, until constant.As contained more insoluble impurities in sample, then sample can be moved into asbestos crucible, drying after weighing, move into ashing in 550 DEG C of High Temperature Furnaces Heating Apparatuss again, make the whole ashing of the material of carbon containing, be placed in moisture eliminator, be cooled to room temperature to weigh, the amount of losing is robust fibre amount.Following calculating:
X＝(G/m)×100
In formula: X---containing coarse-fibred content in sample, %;
The quality of G---the resistates quality of High Temperature Furnaces Heating Apparatus loss (or through), g;
The quality of m---sample, g.
According to national standard method, the cigarette garden beet spices content of cellulose detect the sugar beet juice of squeezing, preparing without cigarette garden beet spices and the inventive method of cellulose treatment, can calculate cellulosic degradation rate, specifically see the following form.
| Sample ID | Mierocrystalline cellulose reduced rate (%) |
| The sugar beet juice of squeezing | 0 |
| Without the cigarette garden beet spices of cellulose treatment | 27.84 |
| Cigarette garden beet spices prepared by the inventive method | 82.53 |
Above-described embodiment is illustrative principle of the present invention and effect thereof only, but not for limiting the present invention.Any person skilled in the art all without prejudice under spirit of the present invention and category, can modify above-described embodiment or changes.Therefore, such as described technical field routine techniques personnel do not depart from complete under disclosed spirit and technological thought all equivalence modify or change, must be contained by claim of the present invention.
Claims (10)
1. a preparation method for cigarette garden beet spices, is characterized in that: comprise the following steps:
Step 1: beet is carried out making beating process, collect slurries, described slurries are sugar beet juice;
Step 2: add distilled water and dissolve in cellulase, after filtering, get filtrate, obtain cellulase solution; Described cellulase solution is added in the sugar beet juice of step 1, carries out ferment treatment reaction, obtain ferment treatment liquid;
Step 3: in regulating step 2, the pH of ferment treatment liquid is to iso-electric point, carries out iso-electric point sedimentation, leaves standstill, obtains ferment treatment sedimentation liquid;
Step 4: ferment treatment sedimentation liquid in step 3 is first carried out coarse filtration, then coarse filtration liquid is carried out essence filter, obtain the sedimentation liquid clarified;
Step 5: the sedimentation liquid of clarification in step 4 is carried out concentrating under reduced pressure, obtains beet spices.
2. the preparation method of cigarette garden beet spices according to claim 1, it is characterized in that: in described step 2, the distilled water added in cellulase and the mass ratio of cellulase are 5:1, after filtering, get filtrate, obtain cellulase solution, when carrying out ferment treatment reaction, the amount ratio of cellulase solution and sugar beet juice is 0.1 ~ 10 gram: 1 liter.
3. the preparation method of cigarette garden beet spices according to claim 1, is characterized in that: in described step 2, ferment treatment reaction pH value be 3 ~ 7, ferment treatment temperature carries out at being 25 ~ 40 DEG C, enzyme processing time is 5 ~ 30 hours.
4. the preparation method of cigarette garden beet spices according to claim 1, is characterized in that: in described step 2, adopts 0.8 micron membrane filter to filter.
5. the preparation method of cigarette garden beet spices according to claim 1, is characterized in that: in described step 3, and the pH value of regulatory enzyme treatment solution is 8 ~ 10.
6. the preparation method of cigarette garden beet spices according to claim 1, is characterized in that: in described step 3, constantly stirs in iso-electric point settling process, and described mixing speed is 6000 ~ 12000 revs/min, and churning time is 10 ~ 30 minutes.
7. the preparation method of cigarette garden beet spices according to claim 1, is characterized in that: in described step 3, and the time left standstill is no less than 12 hours.
8. the preparation method of cigarette garden beet spices according to claim 1, is characterized in that: in described step 4, adopts gauze to carry out coarse filtration, carries out essence filter with 0.8 micron membrane filter.
9. the preparation method of cigarette garden beet spices according to claim 1, is characterized in that: in the concentration process in described step 5, and thickening temperature is 50 ~ 80 DEG C, and concentrated pressure is 0.01 ~ 0.2 normal atmosphere.
10. the preparation method of cigarette garden beet spices according to claim 9, is characterized in that: the relative density being concentrated into beet spices is 1.150 ~ 1.350g/cm
3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510462750.5A CN105112160A (en) | 2015-07-31 | 2015-07-31 | Preparation method for beet flavor for tobacco |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510462750.5A CN105112160A (en) | 2015-07-31 | 2015-07-31 | Preparation method for beet flavor for tobacco |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105112160A true CN105112160A (en) | 2015-12-02 |
Family
ID=54660281
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510462750.5A Pending CN105112160A (en) | 2015-07-31 | 2015-07-31 | Preparation method for beet flavor for tobacco |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105112160A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105533352A (en) * | 2015-12-17 | 2016-05-04 | 安徽砀山海升果业有限责任公司 | Method for preparing concentrated red beet juice by using multi-enzyme preparation |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1931986A (en) * | 2006-10-13 | 2007-03-21 | 华宝食用香精香料（上海）有限公司 | Biological process of converting sugar-containing solution into perfume for cigarette |
| CN102796615A (en) * | 2011-05-24 | 2012-11-28 | 湖北中烟工业有限责任公司 | Method for preparing cigarette strawberry extract by using pectinase |
| CN102952634A (en) * | 2011-08-24 | 2013-03-06 | 湖北中烟工业有限责任公司 | Preparation method for cigarette cherry extract and application of cigarette cherry extract in cigarettes |
| CN103564645A (en) * | 2012-07-18 | 2014-02-12 | 朱大恒 | Method and application for producing tobacco extracts through field tobacco waste |
| CN103666761A (en) * | 2012-08-31 | 2014-03-26 | 朱大恒 | Carrot extract and application thereof in tobacco flavoring and charging |
| CN103695178A (en) * | 2012-09-27 | 2014-04-02 | 朱大恒 | Carrot extract and its application in tobaccos |
-
2015
- 2015-07-31 CN CN201510462750.5A patent/CN105112160A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1931986A (en) * | 2006-10-13 | 2007-03-21 | 华宝食用香精香料（上海）有限公司 | Biological process of converting sugar-containing solution into perfume for cigarette |
| CN102796615A (en) * | 2011-05-24 | 2012-11-28 | 湖北中烟工业有限责任公司 | Method for preparing cigarette strawberry extract by using pectinase |
| CN102952634A (en) * | 2011-08-24 | 2013-03-06 | 湖北中烟工业有限责任公司 | Preparation method for cigarette cherry extract and application of cigarette cherry extract in cigarettes |
| CN103564645A (en) * | 2012-07-18 | 2014-02-12 | 朱大恒 | Method and application for producing tobacco extracts through field tobacco waste |
| CN103666761A (en) * | 2012-08-31 | 2014-03-26 | 朱大恒 | Carrot extract and application thereof in tobacco flavoring and charging |
| CN103695178A (en) * | 2012-09-27 | 2014-04-02 | 朱大恒 | Carrot extract and its application in tobaccos |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105533352A (en) * | 2015-12-17 | 2016-05-04 | 安徽砀山海升果业有限责任公司 | Method for preparing concentrated red beet juice by using multi-enzyme preparation |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107446825B (en) | Tremella fuciformis strain and application thereof | |
| CN101036698B (en) | Mulberry leaf extract and its preparing process and application | |
| CN103965369B (en) | Ganoderan, method for extraction and purification and the application as tobacco humectant thereof | |
| CN105852189A (en) | Method for preparing tobacco extract by utilizing waste and inferior tobacco leaves | |
| CN102181414B (en) | Complex enzyme additive for cigarettes as well as preparation method and application thereof | |
| CN103315387B (en) | Application of Chinese yam extractives in cigarettes | |
| CN105062677A (en) | Method for preparing fermented beet extract through processing beet juice by Lactobacillus plantarum | |
| CN102894443A (en) | Coix seed beverage and preparation method thereof | |
| TWI754125B (en) | Preparation method for high content trehalose | |
| CN106962578A (en) | A kind of polysaccharide containing mulberries and the gel soft candy of matrimony vine and preparation method thereof | |
| CN112029006B (en) | Tremella polysaccharide and preparation method and application thereof | |
| CN104928032A (en) | Processing method of using cellulase to treat pineapple juice to prepare pineapple spice and application of pineapple spice | |
| CN104305510A (en) | Humectant for cigarettes and application thereof | |
| CN105112160A (en) | Preparation method for beet flavor for tobacco | |
| CN104946398B (en) | A method of pineapple juice is handled by arthrobacterium and prepares cigarette pineapple flavors | |
| CN103073657A (en) | Method for manufacturing sunflower low-fat pectins | |
| CN105029687A (en) | Preparing method for cigarette addition agent rich in barley polysaccharide | |
| CN105176678A (en) | Preparation method for beet flavor used for cigarette | |
| CN105001990A (en) | Preparation method of beet extract for cigarettes | |
| CN103772511A (en) | Production process of colocasia esculenta starch | |
| CN105077552A (en) | Application of eleutherococcus brachypus polysaccharide to cigarette as tobacco humectant | |
| CN105062681A (en) | Preparation method for beta vulgaris spice for cigarettes | |
| US2834694A (en) | Fructose polymers and method of preparation | |
| CN104974849B (en) | A kind of preparation method of fermentation type tobacco garden beet medicinal extract | |
| CN104911028A (en) | Preparation method and application of pineapple extract for tobacco |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20151202 |
|
| RJ01 | Rejection of invention patent application after publication |