CN105106024B - Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder and preparation method thereof - Google Patents
Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder and preparation method thereof Download PDFInfo
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- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
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- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/02—Preparations for care of the skin for chemically bleaching or whitening the skin
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Abstract
The present invention relates to flexible nano-liposomes freeze-dried powder and preparation method thereof technical field, it is a kind of Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder and preparation method thereof, preparing the raw material of Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder includes cistanche extracts phenylethanoid glycosides, lecithin, cholesterol and sodium taurocholate.First nano liposomes freeze-dried powder is made in cistanche deserticola phenylethanoid glycosides by the present invention, improve the Transdermal absorption effect of cistanche extracts phenylethanoid glycosides, the action time of cistanche extracts phenylethanoid glycosides is extended at the same time, and Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder according to the present invention has the characteristics that preferable envelop rate, particle diameter be smaller and good stability, the exploitation for being the later stage using cistanche extracts phenylethanoid glycosides as the cosmetics or Related product of main component provides technical basis.
Description
Technical field
The present invention relates to flexible nano-liposomes freeze-dried powder and preparation method thereof technical field, is a kind of Cistanche deserticola extraction
Thing phenylethanoid glycosides flexible nano-liposomes freeze-dried powder and preparation method thereof.
Background technology
Saline cistanche is one of most characteristic advantage medicine resource in Xinjiang, and ancient times multi-section TCM Literature records saline cistanche tool
There is the effect of happy color beauty treatment, seminar takes the lead in having carried out at home saline cistanche in metabolism of pigment obstacle disease and skin maintenance neck
The research in domain, research show that cistanche extracts phenylethanoid glycosides have effects that whitening sun protection is mainly related with following factor：（1）
Suppress tyrosinase activity, reduce melanocyte and formed.Tyrosinase be in melanocyte be catalyzed melanogenesis rate-limiting enzyme, saline cistanche
Extract phenylethanoid glycosides can penetrate into skin, while cell Proliferation concentration is not influenced, can effectively inhibit the junket in skin
The activity of propylhomoserin enzyme, blocks the formation of melanin, so that Skin pigmentation is reduced, dispelling stain and freckle, and to black
Plain cell does not produce the side effects such as toxic, irritation, sensitization.（2）Remove free radical and antioxidation.Research shows：
The catalytic action of tyrosinase and DOPA quinone and the Auto-oxidation reaction occurred later are both needed to the participation of free radical, free radical number
Increase or the enhancing of activity, can accelerate melanogenesis speed.Cistanche extracts phenylethanoid glycosides, which have, removes external oxygen freedom
Base, antioxidation, for its play whitening sun protection effect provide according to (Liu Fengxia, waits Chinese Pharmacological Bulletins, 2006,
22,(5):595-599.).（3）Radiation resistance.Animal experiment study is shown：Cistanche extracts phenylethanoid glycosides external application system
Agent is to ultraviolet B radiation（UVB）Irradiation causes mouse skin pigmentation light Ageing Model to have notable protective effect.Above drug effect
Research, provide foundation for application of the cistanche extracts phenylethanoid glycosides in terms of whitening sun protection cosmetics.
Liposome is that Britain molecular biologist doctor Bangham had found and named first in 1962, liposome be by
The completely enclosed multi-layer vesicles for the bilayer that the lipoids such as phosphatide are formed, its structure are similar to cell, have and are sustained controllable, peace
The features such as Atoxic.And liposome has the structure similar to skin, there is good skin permeability.Research shows, fat
Plastid can go swiftly to deep skin, can stay in more active materials after liposomal encapsulated active material percutaneous absorbtion
Epidermis is between corium.Dior is in the Liposome Cosmeatics for developing first trade name Capture in 1986, its appearance
Indicate the beginning of cosmetics of new generation.In recent decades, American-European and Japan to the ultra-micro carrier system of cosmetic active thing into
Substantial amounts of research is gone, liposome is applied to cosmetic formulations more and more widely.In order to overcome conventional liposome particle diameter not
Uniformly, the shortcomings of unstability, conventional liposome is transformed, flexible nano-liposomes freeze-dried powder, flexible nano fat is made
Application characteristic of the plastid freeze-dried powder in cosmetics is as follows：
（1）Compatibility：Due to lipid bilayer have it is amphiphilic, can by liposomal encapsulated beauty and skin care because
Son can be water miscible (being encapsulated in liposome water phase center), or amphiphilic material (being embedded in lipid bilayer),
Even the different active ingredient of several properties can be encapsulated at the same time.（2）Long-effective protection acts on：Since there is liposome sustained release to make
With, therefore after dermal layer of the skin is entered, encapsulated active factors can be slowly released out, thus substantially prolongs it
Play the time of effect.Liposome Cosmeatics of different nature, its active ingredient are also different in the time that skin endodermis is detained.
The bimolecular lamellar lipid membrane active material unstable to properties has protective effect at the same time, if being encapsulated in liposome
In, then the interference of outside environmental elements is not easily susceptible to, its internal stability and vitro stability have obtained different degrees of carry
It is high.（3）Skin care moisture-keeping function：Phosphatide in liposome has the innate efficacy of the tender skin of moisturizing, without side-effects.When liposome is made up
After cell fusion in product and cortex, the effect of phospholipid composition will play moisturizing tender skin, skin is set to become soft and glossy.
In addition, the various beauty and skin care factors embedded in liposome can also discharge at leisure, the various health protections played to skin are beautiful
Appearance acts on.（4）Particle diameter is small：The nano-lipid carrier of small particle has good compatibility with keratoderma, will efficiently can live
Property component conveys and penetrates into cuticula so that active component effectively acts on the deep layer cells of skin.（5）Stability is good：
Liposome solutions store for a long time can be there are many problems, and aggregation and phosphatide such as the leakage, liposome particles of active material exist
Easily aoxidized under liquid condition, these problems limit application of the liposome in cosmetics.Liposome is made with good
The dry particle and powder of good mobility, mix using preceding with water, you can disperse or dissolve into liposome, solve wrappage and
The stability problem of its own, extends the holding time of Lipidosome.
The content of the invention
The present invention provides a kind of Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder and its preparation
Method, makes public for the first time cistanche extracts phenylethanoid glycosides Cistanche deserticola extract phenylethanoid glycosides flexible nano fat is made
Plastid freeze-dried powder, Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder of the present invention have envelop rate
Preferably, particle diameter is smaller and the characteristics of stability is high.
One of technical scheme is realized by following measures：A kind of Cistanche deserticola extract benzyl carbinol is total
Glycosides flexible nano-liposomes freeze-dried powder, raw materials by weight portion meter include 1 part to 10 parts cistanche extracts phenylethanoid glycosides,
60 parts to 140 parts of lecithin, 10 parts to 90 parts of cholesterol and 10 parts to 120 parts of surfactant, Cistanche deserticola extraction
Thing phenylethanoid glycosides flexible nano-liposomes freeze-dried powder obtains in the steps below：The first step, consolidates to the desired amount of lecithin and courage
Organic solvent is added in alcohol until lecithin and cholesterol obtain lysate after being dissolved completely in organic solvent；Second step, will be molten
Solution liquid is evaporated under reduced pressure, and when thin film is occurring in lysate during reduction vaporization, is added into lysate organic mixed
Solution is closed until oil phase will be obtained after Film Fractionation；3rd step, adds into oil phase and contains the desired amount of cistanche extracts benzene
The phosphate buffer of the total glycosides of ethanol and the phosphate buffer containing the desired amount of surfactant, contain aequum by addition
Cistanche extracts phenylethanoid glycosides phosphate buffer and phosphate buffer containing the desired amount of surfactant
Oil phase carry out sonic oscillation after obtain lotion；4th step, by lotion stand 30 minutes to 40 minutes, when stand 30 minutes to 40
Lotion after minute is when occurring without lamination, will stand 30 minutes and is evaporated under reduced pressure to lotion after forty minutes, to remove
Organic mixed solution in lotion, when lotion is evaporated under reduced pressure to gelatinous, freeze drying protectant aqueous solution water is added into lotion
Hydrate is obtained after conjunction, wherein, hydration time for 1 hour to 1.2 it is small when, lotion and freeze drying protectant aqueous solution volume ratio are 1:
1 to 2；5th step, flexible nano-liposomes suspension is obtained after hydrate is carried out homogenization；6th step, by flexible nano
Liposome turbid liquor obtains Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder after being freeze-dried, its
In, the temperature of freeze-drying is -50 DEG C to -40 DEG C.
Here is the further optimization and/or improvements to one of foregoing invention technical solution：
Above-mentioned lecithin is soybean lecithin, egg yolk lecithin, Distearoyl Phosphatidylcholine, two palmityl phosphatidyl courages
Any one of alkali, dimyristoyl phosphatidyl choline and ceramide；Or/and surfactant is sodium taurocholate or deoxidation
Sodium taurocholate.
Above-mentioned freeze drying protectant is lactose, sucrose, fructose, maltose, trehalose, glucose, mannitol, sorbierite and wood
Any one in sugar alcohol；Or/and phosphate buffer is in the phosphoric acid containing the desired amount of cistanche extracts phenylethanoid glycosides
Content in salt buffer is to be enough to make the benzyl carbinol glycosides in cistanche extracts phenylethanoid glycosides to dissolve, phosphate buffer
PH value is 5 to 7；Or/and content of the phosphate buffer in the phosphate buffer containing the desired amount of surfactant is
It is enough to dissolve surfactant, the pH value of phosphate buffer is 5 to 7；Or/and the quality hundred of freeze drying protectant aqueous solution
It is 10% to 20% to divide specific concentration.
In above-mentioned 5th step, the pressure of homogenization is 10000psi to 60000psi, and the temperature of homogenization is 30 DEG C
To 70 DEG C, the number of homogenization is 1 time to 6 times.
In above-mentioned 6th step, need to carry out pre-freeze, pre-freeze before flexible nano-liposomes suspension is freeze-dried
Temperature be 0 DEG C to 8 DEG C, time of freeze-drying for 6 hours to 48 it is small when；Or/and benzyl carbinol in cistanche glucoside extract
Mass percent of the glycosides in Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder is 1% to 99%；Or/
It is any one in Desert Herba Cistanches, Cistanche tubulosa and halophilous herbage with, Cistanche deserticola.
The two of technical scheme are realized by following measures：A kind of Cistanche deserticola extract benzyl carbinol is total
The preparation method of glycosides flexible nano-liposomes freeze-dried powder, raw materials by weight portion meter include 1 part to 10 parts of cistanche extracts
Phenylethanoid glycosides, 60 parts to 140 parts of lecithin, 10 parts to 90 parts of cholesterol and 10 parts to 120 parts of surfactant, meat
The preparation method of desert cistanche category extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder carries out in the steps below：The first step, to
Organic solvent is added in the desired amount of lecithin and cholesterol until lecithin and cholesterol obtain after being dissolved completely in organic solvent
To lysate；Second step, lysate is evaporated under reduced pressure, when is there is thin film in lysate during reduction vaporization,
Organic mixed solution is added into lysate until oil phase will be obtained after Film Fractionation；3rd step, adds containing into oil phase
The phosphate buffer of the cistanche extracts phenylethanoid glycosides of requirement and the phosphate containing the desired amount of surfactant delay
Fliud flushing, will add the phosphate buffer containing the desired amount of cistanche extracts phenylethanoid glycosides and contains the desired amount of surface
The oil phase of the phosphate buffer of activating agent obtains lotion after carrying out sonic oscillation；4th step, 30 minutes to 40 are stood by lotion
Minute, when standing occurs without lamination in 30 minutes to lotion after forty minutes, 30 minutes will be stood to breast after forty minutes
Liquid is evaporated under reduced pressure, and to remove organic mixed solution in lotion, when lotion is evaporated under reduced pressure to gelatinous, is added into lotion
Obtain hydrate after entering the hydration of freeze drying protectant aqueous solution, wherein, hydration time for 1 hour to 1.2 it is small when, lotion is protected with lyophilized
It is 1 to protect agent aqueous solution volume ratio:1 to 2；5th step, flexible nano-liposomes suspension is obtained after hydrate is carried out homogenization
Liquid；6th step, it is soft to obtain Cistanche deserticola extract phenylethanoid glycosides after flexible nano-liposomes suspension is freeze-dried
Property nano liposomes freeze-dried powder, wherein, the temperature of freeze-drying is -50 DEG C to -40 DEG C.
Here is two further optimization and/or improvements to foregoing invention technical solution：
Above-mentioned lecithin is soybean lecithin, egg yolk lecithin, Distearoyl Phosphatidylcholine, two palmityl phosphatidyl courages
Any one of alkali, dimyristoyl phosphatidyl choline and ceramide；Or/and surfactant is sodium taurocholate or deoxidation
Sodium taurocholate.
Above-mentioned freeze drying protectant is lactose, sucrose, fructose, maltose, trehalose, glucose, mannitol, sorbierite and wood
Any one in sugar alcohol；Or/and phosphate buffer is in the phosphoric acid containing the desired amount of cistanche extracts phenylethanoid glycosides
Content in salt buffer is to be enough to make the benzyl carbinol glycosides in cistanche extracts phenylethanoid glycosides to dissolve, phosphate buffer
PH value is 5 to 7；Or/and content of the phosphate buffer in the phosphate buffer containing the desired amount of surfactant is
It is enough to dissolve surfactant, the pH value of phosphate buffer is 5 to 7；Or/and the quality hundred of freeze drying protectant aqueous solution
It is 10% to 20% to divide specific concentration.
In above-mentioned 5th step, the pressure of homogenization is 10000psi to 60000psi, and the temperature of homogenization is 30 DEG C
To 70 DEG C, the number of homogenization is 1 time to 6 times.
In above-mentioned 6th step, need to carry out pre-freeze, pre-freeze before flexible nano-liposomes suspension is freeze-dried
Temperature be 0 DEG C to 8 DEG C, time of freeze-drying for 6 hours to 48 it is small when；Or/and benzyl carbinol in cistanche glucoside extract
Mass percent of the glycosides in Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder is 1% to 99%；Or/
It is any one in Desert Herba Cistanches, Cistanche tubulosa and halophilous herbage with, Cistanche deserticola.
First nano liposomes freeze-dried powder is made in cistanche deserticola phenylethanoid glycosides by the present invention, improves cistanche extracts benzene
The Transdermal absorption effect of the total glycosides of ethanol, while the action time of cistanche extracts phenylethanoid glycosides is extended, and according to this
The invention Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder have preferable envelop rate, particle diameter compared with
The characteristics of small and good stability is the later stage using cistanche extracts phenylethanoid glycosides as the cosmetics of main component or related production
The exploitation of product provides technical basis.
Brief description of the drawings
Attached drawing 1 is the Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder that embodiment 3 obtains（It is multiple
It is molten）Electron microscope.
Embodiment
The present invention from following embodiments limitation, can technique according to the invention scheme and actual conditions determine specifically
Embodiment.
With reference to embodiment, the invention will be further described：
Embodiment 1：The Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder, raw materials by weight portion
The cistanche extracts phenylethanoid glycosides, 60 parts to 140 parts of lecithin, 10 parts to 90 parts of courage that meter includes 1 part to 10 parts are consolidated
Alcohol and 10 parts to 120 parts of surfactant, Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder are pressed
Preparation method is stated to obtain：The first step, organic solvent is added until lecithin and courage are consolidated into the desired amount of lecithin and cholesterol
Alcohol obtains lysate after being dissolved completely in organic solvent；Second step, lysate is evaporated under reduced pressure, when lysate is steamed in decompression
When occurring thin film during hair, organic mixed solution is added into lysate until oil phase will be obtained after Film Fractionation；The
Three steps, the phosphate buffer containing the desired amount of cistanche extracts phenylethanoid glycosides is added into oil phase and contains aequum
Surfactant phosphate buffer, delay adding containing the phosphate of the desired amount of cistanche extracts phenylethanoid glycosides
The oil phase of fliud flushing and phosphate buffer containing the desired amount of surfactant obtains lotion after carrying out sonic oscillation；4th
Step, 30 minutes to 40 minutes are stood by lotion, will be quiet when standing occurs without lamination in 30 minutes to lotion after forty minutes
Put 30 minutes and be evaporated under reduced pressure to lotion after forty minutes, to remove organic mixed solution in lotion, steamed when lotion depressurizes
When being sent to gelatinous, hydrate is obtained after the hydration of freeze drying protectant aqueous solution is added into lotion, wherein, hydration time is small for 1
When 1.2 is small, lotion is 1 with freeze drying protectant aqueous solution volume ratio:1 to 2；5th step, homogenization is carried out by hydrate
After obtain flexible nano-liposomes suspension；6th step, meat is obtained after flexible nano-liposomes suspension is freeze-dried
Desert cistanche category extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder, wherein, the temperature of freeze-drying is -50 DEG C to -40 DEG C.
The envelop rate of the Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder obtained according to the present embodiment（%）, grain
Footpath distribution（nm）And average grain diameter（nm）As shown in table 1.The Cistanche deserticola extract benzene second that will be obtained according to the present embodiment
The total glycosides flexible nano-liposomes freeze-dried powder of alcohol temperature be 4 DEG C in the environment of places 30 days after observe, observe the results show that according to
The Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes that the present embodiment obtains freeze powder and agglomeration phenomena, explanation occur
The Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder that the present embodiment obtains is with good stability.By
Exist in solid form in the Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder that the present embodiment obtains, no
It only ensure that stability, and avoid phosphatide（Lecithin）Oxydrolysis, so as to be effectively improved cistanche extracts
The Transdermal absorption effect of phenylethanoid glycosides, while extend medicine（Cistanche extracts phenylethanoid glycosides）Action time.This
The Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder that embodiment obtains takes full advantage of nano liposomes
Stablize, be sustained the features such as controllable, bioavilability is high and safe and harmless.
Embodiment 2：The Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder, raw materials by weight portion
The cistanche extracts phenylethanoid glycosides, the lecithin of 60 parts or 140 parts, the courage of 10 parts or 90 parts that meter includes 1 part or 10 parts are consolidated
Alcohol and 10 parts or 120 parts of surfactant, Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder are pressed
Preparation method is stated to obtain：The first step, organic solvent is added until lecithin and courage are consolidated into the desired amount of lecithin and cholesterol
Alcohol obtains lysate after being dissolved completely in organic solvent；Second step, lysate is evaporated under reduced pressure, when lysate is steamed in decompression
When occurring thin film during hair, organic mixed solution is added into lysate until oil phase will be obtained after Film Fractionation；The
Three steps, the phosphate buffer containing the desired amount of cistanche extracts phenylethanoid glycosides is added into oil phase and contains aequum
Surfactant phosphate buffer, delay adding containing the phosphate of the desired amount of cistanche extracts phenylethanoid glycosides
The oil phase of fliud flushing and phosphate buffer containing the desired amount of surfactant obtains lotion after carrying out sonic oscillation；4th
Step, 30 minutes or 40 minutes are stood by lotion, will be quiet when standing 30 minutes or lotion after forty minutes occurs without lamination
Put 30 minutes or lotion after forty minutes is evaporated under reduced pressure, to remove organic mixed solution in lotion, steamed when lotion depressurizes
When being sent to gelatinous, hydrate is obtained after the hydration of freeze drying protectant aqueous solution is added into lotion, wherein, hydration time is small for 1
When or 1.2 it is small when, lotion and freeze drying protectant aqueous solution volume ratio are 1:1 or 2；5th step, homogenization is carried out by hydrate
After obtain flexible nano-liposomes suspension；6th step, meat is obtained after flexible nano-liposomes suspension is freeze-dried
Desert cistanche category extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder, wherein, the temperature of freeze-drying is -50 DEG C or -40 DEG C.
Embodiment 3：The Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder, raw materials by weight portion
The cistanche extracts phenylethanoid glycosides, 80 parts to 100 parts of lecithin, 60 parts to 90 parts of courage that meter includes 6 parts to 10 parts are consolidated
Alcohol and 80 parts to 110 parts of surfactant, Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder are pressed
Preparation method is stated to obtain：The first step, organic solvent is added until lecithin and courage are consolidated into the desired amount of lecithin and cholesterol
Alcohol obtains lysate after being dissolved completely in organic solvent；Second step, lysate is evaporated under reduced pressure, when lysate is steamed in decompression
When occurring thin film during hair, organic mixed solution is added into lysate until oil phase will be obtained after Film Fractionation；The
Three steps, the phosphate buffer containing the desired amount of cistanche extracts phenylethanoid glycosides is added into oil phase and contains aequum
Surfactant phosphate buffer, delay adding containing the phosphate of the desired amount of cistanche extracts phenylethanoid glycosides
The oil phase of fliud flushing and phosphate buffer containing the desired amount of surfactant obtains lotion after carrying out sonic oscillation；4th
Step, 30 minutes are stood by lotion, when the lotion after standing 30 minutes occurs without lamination, by the lotion after standing 30 minutes
It is evaporated under reduced pressure, to remove organic mixed solution in lotion, when lotion is evaporated under reduced pressure to gelatinous, is added into lotion
Hydrate is obtained after the hydration of freeze drying protectant aqueous solution, wherein, when hydration time is 1 small, lotion and freeze drying protectant aqueous solution
Volume ratio is 1:1；5th step, flexible nano-liposomes suspension is obtained after hydrate is carried out homogenization；6th step, will be soft
Property nanometer liposome turbid liquor be freeze-dried after obtain Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes jelly
Dry powder, wherein, the temperature of freeze-drying is -45 DEG C to -40 DEG C.The Cistanche deserticola extract benzyl carbinol obtained according to the present embodiment
The envelop rate of total glycosides flexible nano-liposomes freeze-dried powder（%）, particle size distribution range（nm）And average grain diameter（nm）As shown in table 1,
In temperature it is 4 DEG C by the Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder that is obtained according to the present embodiment
After being placed 30 days under environment, the Cistanche deserticola extract phenylethanoid glycosides that are obtained after redissolution by electron microscopic observation according to the present embodiment
Flexible nano-liposomes freeze-dried powder, its electron microscope is as shown in Figure 1, as seen in Figure 1, the Cistanche deserticola that the present embodiment obtains
Each cistanche extracts phenylethanoid glycosides flexible nano fat in extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder
Plastid（Black pointing object in Fig. 1）Dispersed distribution, does not occur agglomeration phenomena, illustrates the Cistanche deserticola extraction that the present embodiment obtains
Thing phenylethanoid glycosides flexible nano-liposomes freeze-dried powder is with good stability.
Embodiment 4：The Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder, raw materials by weight portion
Meter includes 2 parts to 5 parts of cistanche extracts phenylethanoid glycosides, 120 parts to 140 parts of lecithin, 11.1 parts to 50 parts of courage
Sterol and 11.1 parts to 60 parts of surfactant, Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder are pressed
Following preparation methods obtain：The first step, organic solvent is added until lecithin and courage into the desired amount of lecithin and cholesterol
Sterol obtains lysate after being dissolved completely in organic solvent；Second step, lysate is evaporated under reduced pressure, when lysate is depressurizing
When occurring thin film in evaporation process, organic mixed solution is added into lysate until oil phase will be obtained after Film Fractionation；
3rd step, the phosphate buffer containing the desired amount of cistanche extracts phenylethanoid glycosides is added into oil phase and containing required
The phosphate buffer of the surfactant of amount, will add the phosphate containing the desired amount of cistanche extracts phenylethanoid glycosides
The oil phase of buffer solution and phosphate buffer containing the desired amount of surfactant obtains lotion after carrying out sonic oscillation；4th
Step, 40 minutes are stood by lotion, when the lotion of standing after forty minutes occurs without lamination, will stand lotion after forty minutes
It is evaporated under reduced pressure, to remove organic mixed solution in lotion, when lotion is evaporated under reduced pressure to gelatinous, is added into lotion
Obtain hydrate after the hydration of freeze drying protectant aqueous solution, wherein, hydration time for 1 hour to 1.2 it is small when, lotion and frozen-dried protective
Agent aqueous solution volume ratio is 1:2；5th step, flexible nano-liposomes suspension is obtained after hydrate is carried out homogenization；The
Six steps, obtain Cistanche deserticola extract phenylethanoid glycosides flexible nano after flexible nano-liposomes suspension is freeze-dried
Lipidosome freeze-dried powder, wherein, the temperature of freeze-drying is -46 DEG C.The Cistanche deserticola extract benzene second obtained according to the present embodiment
The envelop rate of the total glycosides flexible nano-liposomes freeze-dried powder of alcohol（%）, particle size distribution range（nm）And average grain diameter（nm）Such as the institute of table 1
Show, by the Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder obtained according to the present embodiment temperature be 4
After being placed 30 days in the environment of DEG C, the Cistanche deserticola extract phenylethanoid glycosides flexible nano fat obtained according to the present embodiment is observed
Plastid freezes powder and agglomeration phenomena occurs, illustrates the Cistanche deserticola extract phenylethanoid glycosides flexible nano fat that the present embodiment obtains
Plastid freeze-dried powder is with good stability.
Embodiment 5：It is with the difference of above-described embodiment, lecithin is soybean lecithin, egg yolk lecithin, two hard
Any one of acyl phosphatidyl choline, dipalmitoylphosphatidylcholine, dimyristoyl phosphatidyl choline and ceramide；
Or/and surfactant is sodium taurocholate or NaTDC.
Embodiment 6：Be with the difference of above-described embodiment, freeze drying protectant for lactose, sucrose, fructose, maltose,
Any one in trehalose, glucose, mannitol, sorbierite and xylitol；Or/and phosphate buffer is containing required
Content in the phosphate buffer of the cistanche extracts phenylethanoid glycosides of amount is to be enough to make cistanche extracts benzyl carbinol total
Benzyl carbinol glycosides dissolving in glycosides, the pH value of phosphate buffer is 5 to 7；Or/and phosphate buffer is containing the desired amount of
For content in the phosphate buffer of surfactant to be enough to dissolve surfactant, the pH value of phosphate buffer is 5
To 7；Or/and the mass percent concentration of freeze drying protectant aqueous solution is 10% to 20%.
Embodiment 7：It is with the difference of above-described embodiment, in the 5th step, the pressure of homogenization is 10000psi
To 60000psi, the temperature of homogenization is 30 DEG C to 70 DEG C, and the number of homogenization is 1 time to 6 times.
Embodiment 8：Be with the difference of above-described embodiment, in the 6th step, flexible nano-liposomes suspension into
Need to carry out pre-freeze before row freeze-drying, the temperature of pre-freeze is 0 DEG C to 8 DEG C, and the time of freeze-drying is 6 hours to 48 small
When；Or/and benzyl carbinol glycosides freeze in Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes in cistanche glucoside extract
Mass percent in dry powder is 1% to 99%；Or/and Cistanche deserticola is Desert Herba Cistanches, Cistanche tubulosa and halophilous herbage
In any one.
It can be seen that from the envelop rate in table 1, particle size distribution range and average grain diameter according to embodiment 1,3 and of embodiment
The Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder that embodiment 4 obtains has preferable envelop rate and grain
The less feature in footpath, it is achieved thereby that medicine（Cistanche extracts phenylethanoid glycosides）Carrying and sustained release, and then improve life
Thing utilization rate.
In conclusion first nano liposomes freeze-dried powder is made in cistanche deserticola phenylethanoid glycosides by the present invention, meat desert is improved
The Transdermal absorption effect of Rong's extract phenylethanoid glycosides, while the action time of cistanche extracts phenylethanoid glycosides is extended,
And Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder according to the present invention has preferable bag
Envelope rate, particle diameter be smaller and is change of the later stage using cistanche extracts phenylethanoid glycosides as main component the characteristics of good stability
The exploitation of cosmetic or Related product provides technical basis.
Above technical characteristic constitutes the embodiment of the present invention, it, can basis with stronger adaptability and implementation result
It is actually needed and increases and decreases non-essential technical characteristic, meets the needs of different situations.
Table 1
| Envelop rate（%） | Particle size distribution range（nm） | Average grain diameter（nm） | |
| Embodiment 1 | 38% to 40% | 100 to 1000 | 182 to 200 |
| Embodiment 3 | 38% | 200 to 1000 | 182 |
| Embodiment 4 | 40% | 150 to 950 | 200 |
Claims (10)
1. a kind of Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder, it is characterised in that raw material is by weight
Number meter includes 1 part to 10 parts of cistanche extracts phenylethanoid glycosides, 60 parts to 140 parts of lecithin, 10 parts to 90 parts of courage
Sterol and 10 parts to 120 parts of surfactant, surfactant are sodium taurocholate or NaTDC, Cistanche deserticola extract benzene
The total glycosides flexible nano-liposomes freeze-dried powder of ethanol obtains in the steps below：The first step, into the desired amount of lecithin and cholesterol
Organic solvent is added until lecithin and cholesterol obtain lysate after being dissolved completely in organic solvent；Second step, by lysate
It is evaporated under reduced pressure, when thin film is occurring in lysate during reduction vaporization, it is molten that organic mixing is added into lysate
Liquid is until oil phase will be obtained after Film Fractionation；3rd step, adds into oil phase and contains the desired amount of cistanche extracts benzyl carbinol
The phosphate buffer of total glycosides and the phosphate buffer containing the desired amount of surfactant, contain the desired amount of meat by adding
The oil of the phosphate buffer of desert cistanche extract phenylethanoid glycosides and the phosphate buffer containing the desired amount of surfactant
Lotion is obtained after mutually carrying out sonic oscillation；4th step, by lotion stand 30 minutes to 40 minutes, when stand 30 minutes to 40 minutes
When lotion afterwards occurs without lamination, 30 minutes will be stood and be evaporated under reduced pressure to lotion after forty minutes, to remove lotion
In organic mixed solution, when lotion is evaporated under reduced pressure to gelatinous, after the hydration of freeze drying protectant aqueous solution is added into lotion
Obtain hydrate, wherein, hydration time for 1 hour to 1.2 it is small when, lotion and freeze drying protectant aqueous solution volume ratio are 1:1 to
2；5th step, flexible nano-liposomes suspension is obtained after hydrate is carried out homogenization；6th step, by flexible nano lipid
Body suspension obtains Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder after being freeze-dried, wherein,
The temperature of freeze-drying is -50 DEG C to -40 DEG C.
2. Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder according to claim 1, its feature
It is lecithin for soybean lecithin, egg yolk lecithin, Distearoyl Phosphatidylcholine, dipalmitoylphosphatidylcholine, two meat
Any one of cardamom phosphatidyl choline and ceramide.
3. Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder according to claim 1 or 2, it is special
Sign in freeze drying protectant in being lactose, sucrose, fructose, maltose, trehalose, glucose, mannitol, sorbierite and xylitol
Any one；Or/and phosphate buffer is in the phosphate-buffered containing the desired amount of cistanche extracts phenylethanoid glycosides
Content in liquid is to be enough to make the benzyl carbinol glycosides in cistanche extracts phenylethanoid glycosides to dissolve, and the pH value of phosphate buffer is
5 to 7；Or/and content of the phosphate buffer in the phosphate buffer containing the desired amount of surfactant is to be enough to make
Surfactant dissolves, and the pH value of phosphate buffer is 5 to 7；Or/and the mass percent of freeze drying protectant aqueous solution is dense
Spend for 10% to 20%.
4. Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder according to claim 1 or 2, it is special
Sign is in the 5th step, and the pressure of homogenization is 10000psi to 60000psi, and the temperature of homogenization is 30 DEG C to 70 DEG C,
The number of homogenization is 1 time to 6 times.
5. Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder according to claim 3, its feature
In the 5th step, the pressure of homogenization is 10000psi to 60000psi, and the temperature of homogenization is 30 DEG C to 70 DEG C,
Matter number of processing is 1 time to 6 times.
6. Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder according to claim 1 or 2, it is special
Sign in the 6th step, is needing to carry out pre-freeze, the temperature of pre-freeze before flexible nano-liposomes suspension is freeze-dried
For 0 DEG C to 8 DEG C, time of freeze-drying for 6 hours to 48 it is small when；Or/and in cistanche glucoside extract benzyl carbinol glycosides in meat
Mass percent in desert cistanche category extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder is 1% to 99%；Or/and meat desert
Rong belongs to for any one in Desert Herba Cistanches, Cistanche tubulosa and halophilous herbage.
7. Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder according to claim 3, its feature
In the 6th step, needing to carry out pre-freeze before flexible nano-liposomes suspension is freeze-dried, the temperature of pre-freeze is 0
DEG C to 8 DEG C, time of freeze-drying for 6 hours to 48 it is small when；Or/and in cistanche glucoside extract benzyl carbinol glycosides in meat desert
The mass percent that Rong belongs in extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder is 1% to 99%.
8. Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder according to claim 4, its feature
In the 6th step, needing to carry out pre-freeze before flexible nano-liposomes suspension is freeze-dried, the temperature of pre-freeze is 0
DEG C to 8 DEG C, time of freeze-drying for 6 hours to 48 it is small when；Or/and in cistanche glucoside extract benzyl carbinol glycosides in meat desert
The mass percent that Rong belongs in extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder is 1% to 99%.
9. Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder according to claim 5, its feature
In the 6th step, needing to carry out pre-freeze before flexible nano-liposomes suspension is freeze-dried, the temperature of pre-freeze is 0
DEG C to 8 DEG C, time of freeze-drying for 6 hours to 48 it is small when；Or/and in cistanche glucoside extract benzyl carbinol glycosides in meat desert
The mass percent that Rong belongs in extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder is 1% to 99%.
10. a kind of Cistanche deserticola extract benzyl carbinol according to claim 1 or 2 or 3 or 4 or 5 or 6 or 7 or 8 or 9 is total
The preparation method of glycosides flexible nano-liposomes freeze-dried powder, it is characterised in that raw materials by weight portion meter includes 1 part to 10 parts of meat
Desert cistanche extract phenylethanoid glycosides, 60 parts to 140 parts of lecithin, 10 parts to 90 parts of cholesterol and 10 parts to 120 parts of table
Face activating agent, surfactant are sodium taurocholate or NaTDC, Cistanche deserticola extract phenylethanoid glycosides flexible nano lipid
The preparation method of body freeze-dried powder carries out in the steps below：The first step, adds organic molten into the desired amount of lecithin and cholesterol
Agent is until lecithin and cholesterol obtain lysate after being dissolved completely in organic solvent；Second step, decompression steaming is carried out by lysate
Hair, when thin film is occurring in lysate during reduction vaporization, organic mixed solution is added into lysate until will be thin
Oil phase is obtained after film dissolving；3rd step, the phosphoric acid containing the desired amount of cistanche extracts phenylethanoid glycosides is added into oil phase
Salt buffer and the phosphate buffer containing the desired amount of surfactant, contain the desired amount of cistanche extracts by adding
The oil phase of the phosphate buffer of phenylethanoid glycosides and phosphate buffer containing the desired amount of surfactant carries out ultrasound
Lotion is obtained after vibration；4th step, by lotion stand 30 minutes to 40 minutes, when stand 30 minutes to lotion after forty minutes not
When there is lamination, 30 minutes will be stood and be evaporated under reduced pressure to lotion after forty minutes, it is organic mixed in lotion to remove
Solution is closed, when lotion is evaporated under reduced pressure to gelatinous, hydrate is obtained after the hydration of freeze drying protectant aqueous solution is added into lotion,
Wherein, hydration time for 1 hour to 1.2 it is small when, lotion and freeze drying protectant aqueous solution volume ratio are 1:1 to 2；5th step, will
Hydrate obtains flexible nano-liposomes suspension after carrying out homogenization；6th step, by flexible nano-liposomes suspension into
Cistanche deserticola extract phenylethanoid glycosides flexible nano-liposomes freeze-dried powder is obtained after row freeze-drying, wherein, freeze-drying
Temperature is -50 DEG C to -40 DEG C.
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| CN105534898A (en) * | 2015-12-31 | 2016-05-04 | 杨建华 | Nano-emulsion of phenylethanoid glycosides in cistanche salsa and preparation method thereof |
| CN107982132A (en) * | 2017-12-24 | 2018-05-04 | 姚佑灿 | A kind of radioresistance isolation breast |
| CN107998183B (en) * | 2017-12-26 | 2021-05-04 | 浙江大学 | Osmanthus fragrans phenylethanoid glycoside liposome coated with chitosan and preparation method thereof |
| CN108904369A (en) * | 2018-05-03 | 2018-11-30 | 扬州梦晓生物工程有限公司 | It is a kind of to load micro- peptide nano liposomes and its preparation, application and application method |
| CN110464835B (en) * | 2018-05-11 | 2023-06-16 | 中国医学科学院药物研究所 | Insulin flexible particles and preparation thereof |
| CN110169560B (en) * | 2019-06-19 | 2021-08-17 | 武汉轻工大学 | Spice extract and spice extract liposome as well as preparation method and application thereof |
| CN110478379B (en) * | 2019-06-21 | 2022-02-18 | 福建医科大学 | Selaginella chinensis total biflavone precursor liposome and preparation method thereof |
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