CN105044297A - Device and method for detecting meat food safety - Google Patents
Device and method for detecting meat food safety Download PDFInfo
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- CN105044297A CN105044297A CN201510303837.8A CN201510303837A CN105044297A CN 105044297 A CN105044297 A CN 105044297A CN 201510303837 A CN201510303837 A CN 201510303837A CN 105044297 A CN105044297 A CN 105044297A
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- 238000001514 detection method Methods 0.000 claims abstract description 59
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- 238000002474 experimental method Methods 0.000 claims abstract description 11
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- 238000012360 testing method Methods 0.000 claims description 45
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- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 claims description 5
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- 229960001117 clenbuterol Drugs 0.000 claims description 3
- STJMRWALKKWQGH-UHFFFAOYSA-N clenbuterol Chemical compound CC(C)(C)NCC(O)C1=CC(Cl)=C(N)C(Cl)=C1 STJMRWALKKWQGH-UHFFFAOYSA-N 0.000 claims description 3
- 239000007789 gas Substances 0.000 claims description 3
- 229960002518 gentamicin Drugs 0.000 claims description 3
- 150000007660 quinolones Chemical class 0.000 claims description 3
- YJQZYXCXBBCEAQ-UHFFFAOYSA-N ractopamine Chemical compound C=1C=C(O)C=CC=1C(O)CNC(C)CCC1=CC=C(O)C=C1 YJQZYXCXBBCEAQ-UHFFFAOYSA-N 0.000 claims description 3
- 229940074095 ractopamine Drugs 0.000 claims description 3
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- NHDHVHZZCFYRSB-UHFFFAOYSA-N pyriproxyfen Chemical compound C=1C=CC=NC=1OC(C)COC(C=C1)=CC=C1OC1=CC=CC=C1 NHDHVHZZCFYRSB-UHFFFAOYSA-N 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a device and a method for detecting meat food safety. The device comprises a detection tank and detection equipment, wherein the detection equipment is positioned inside the detection tank; the detection tank comprises a double-layer tank body and a tank cover movably connected with the double-layer tank body; the detection equipment comprises a sample pretreatment device, a colloidal gold rapid detection card, an experiment reagent, reading and data treatment equipment and lab supplies; one side of the tank body comprises wheel bodies and the opposite other side of the tank body is provided with a flexible pull rod; the wheel bodies are four polyurethane weighted idler wheels; the tank body is in an upper and lower two-layer structure; the lower layer is in a drawer type structure; the upper-lower structure of the tank body and the inside of the tank cover form a storing cavity respectively; the storing cavities are filled with a support module made of a novel environment-friendly plastic foaming material; the surfaces of the support modules are provided with positioning slots with the shape same as the detection equipment and the positioning slots are respectively embedded with and hold the detection equipment.
Description
Technical field
The present invention relates to a kind of detection field of meat food safety, particularly relating to a kind of devices and methods therefor for detecting meat food safety.
Background technology
Meat is the important foodstuffs in people's diet. along with end and the accession to WTO of China's shortage economy, its quality and safety come into one's own day by day. existing management system is unsound, lack efficiently, fast the problem such as detection means become increasingly conspicuous.Particularly in recent years, the widespread use of agricultural chemicals, veterinary drug, feed, feed addictive, animals and plants hormone etc., while promotion agricultural and animal products increase substantially in a large number, also brings the hidden danger of agricultural and animal products quality safety.In addition trade refuse and domestic waste are to the pollution of agriculture production environment, the exceed standard agricultural and animal products that cause of residues of pesticides, residue of veterinary drug and other poisonous and harmful substances pollute and meat product poisoning at home and abroad occurs time and again, the serious threat healthy and life security of consumer, thus, the quality of meat product and safety have become the focal issue of social extensive concern.
China's meat product industry development is rapid, the outstanding problem but microbiotic, violated veterinary drug exceed standard etc., serious restriction China's animal husbandry development and jeopardize the healthy of consumer.Traditional instrumental method is as chromatography or chromatograph-mass spectrometer coupling technology assay method is loaded down with trivial details, efficiency is low, is not suitable for the quick detection of a large amount of sample.ELISA method is the competitive immunization detection method utilizing enzyme marker, and be widely used as quick screening method that is qualitative or half-quantitative detection in recent years, although shorten detection time to a certain extent, operating process is still more complicated.More importantly, above detection method is laboratory method, is difficult to apply at the scene, is unfavorable for promoting the use of.At present, not only laboratory is confined to the detection of meat product, but expand to the links such as production, cultivation, circulation, and time-bounded requirement is higher, therefore in the urgent need to complete checkout equipment and the analytical approach of portability, test in laboratory can be substituted to a certain extent, reach the effect of primary dcreening operation.
Therefore, a kind of device of field quick detection meat products safety is provided to provide.
Summary of the invention
For the deficiencies in the prior art, the invention provides the invention provides one can field quick detection meat food safety devices and methods therefor, structure is simple, cost is low, easy and simple to handle.
A kind of device for detecting meat food safety, described device comprises detection case and checkout equipment, it is inner that described checkout equipment is positioned at described detection case, the case lid that described detection case comprises double-layer box body and is flexibly connected with it, described checkout equipment comprises sample pre-treatments equipment, collaurum rapid detection card, experiment reagent, read and data processing equipment and test consumptive material, the side of described casing comprises wheel body, opposite side is corresponding thereto provided with telescopic lever, described wheel body is that 4 polyurethane increase the weight of roller, described casing is upper and lower double-layer structure, wherein lower floor is drawer-type structure, levels structure and the case lid inside of described casing form glove cavity respectively, wherein be filled with the supporting mould of novel environment friendly plastics expanded material material, be provided with the consistent locating slot of same checkout equipment shape on the surface of described supporting mould, inlay respectively and hold described checkout equipment.
Preferably, described sample pre-treatments equipment comprises that sample preparation is cut, sample preparation cutter, marking pen, sample spoon, operation instructions, micro balance, pipettor, single track timer, vortex centrifugal all-in-one, tissue sample multiplication of voltage rapid treatment instrument, full Self-controllable level instruction nitrogen purges instrument;
Described collaurum rapid detection card comprises sulfamido test card, quinolones test card, chloromycetin test card, gentamicin test card, Clenbuterol test card, Ractopamine test card, salbutamol test card;
Described reading and data processing equipment comprise eight passage collaurum detector and work computer;
Described experiment consumptive material comprises emgloves, centrifuge tube, accurate pH test paper, rifle head, dropper, lab-gown.
Preferably, described detection case is made up of polypropylene composite material, and the eucaryotic cell structure of described polypropylene composite material, for opening nucleus, is engaged between each cell mutually; The casing of described detection case or case lid are also provided with multiple dual control snap close.
Preferably, described dual control snap close is 7, is evenly arranged on the edge of described case lid.
Preferably, also O-ring seal is provided with at the edge of described case lid; Described detection case also comprises gas balance valve.
Preferably, the supporting mould of described case lid internal cavities is disposed with work computer locating slot by order from left to right, from top to bottom, pipettor locating slot, sample preparation cutter locating slot, sample spoon locating slot, marking pen locating slot, sample preparation cut locating slot, micro balance locating slot and timer locating slot;
The supporting mould of described box house upper strata cavity is disposed with collaurum rapid detection card locating slot, vortex centrifugal all-in-one locating slot, eight passage collaurum detector locating slots, Sample Grinder locating slot by order from left to right, from top to bottom; The supporting mould of lower floor's drawer-type structure internal cavity is disposed with full Self-controllable level instruction nitrogen by order from left to right, from top to bottom and purges instrument locating slot, tissue sample multiplication of voltage rapid treatment instrument locating slot, experiment consumptive material locating slot;
Wherein, described multiple locating slot holds corresponding checkout equipment, and described operation instructions are placed in described work computer locating slot together with work computer.
Adopting a detection method for the above-mentioned device for detecting meat food safety, comprising the steps:
1) test card and sample to be checked are returned to room temperature, then take out collaurum rapid detection card
2) use sample extraction instrument, sampling, marking pen marks; Take 2-5g in centrifuge tube after organizing sample to pulverize, add sample extracting solution, with the mixing of vortex centrifugal all-in-one, the centrifugal 10-15min of 4000r/min, gets supernatant, to be checked;
Described extracting tool comprises that sample preparation is cut, sample preparation cutter, sample spoon and micro balance, Sample Grinder;
3) collaurum rapid detection card is kept flat, draw the measuring samples solution after process with pipettor or dropper, vertically dropwise add 5-7 and drip in well, start after application of sample to use timer timing;
4) after 5 minutes, test card is put into eight passage collaurum detectors and read data, carry out data processing with notebook computer.
Technical scheme of the present invention has following beneficial effect:
A kind of devices and methods therefor for detecting meat food safety provided by the invention, configure complete, by sample pre-treatments equipment, detect reagent, test card, reading and data processing equipment integrate, and are applicable to the on-site quick screening of meat products safety, thus reach hazardous material mass-energy Timeliness coverage and monitoring management; There is miniaturization, the advantage such as portable, easy to use; Improve sample pre-treatments efficiency; Dual-use, saves case intracorporeal space and cost; Described wheel body is that 4 polyurethane increase the weight of roller, can bear larger weight, compact, conveniently moving, destination can be moved to rapidly and carry out Site Detection, there is the advantages such as good buffering, antidetonation, heat insulation, moistureproof, resist chemical, and nontoxic, do not absorb water.
Accompanying drawing explanation
Below by drawings and Examples, technical scheme of the present invention is described in further detail.
Fig. 1 is the described detection case structural representation being in open mode under one embodiment of the present invention;
Fig. 2 is dual control buckle structure schematic diagram of the present invention;
Fig. 3 is the structural representation under the cramp of dual control snap close of the present invention and fixture block are in opening.
Embodiment
In order to have a clear understanding of technical scheme of the present invention, its detailed structure will be proposed in the following description.Obviously, the concrete execution of the embodiment of the present invention also not enough specific details being limited to those skilled in the art and haveing the knack of.The preferred embodiments of the present invention are described in detail as follows, and except these embodiments described in detail, can also have other embodiments.
The invention will be further described below:
Embodiment 1
As shown in Figure 1, a kind of device for detecting meat food safety, comprises the checkout equipment in detection case and portion placed within, the case lid 1 that described detection case comprises casing 2 and is flexibly connected with it, preferably, described casing 2 and case lid 1 are connected for side and are flexibly connected, and can freely open and fasten; Described casing 2 and case lid 1 can reach the object of fastening in many ways, as the edge except connection side arranges slide fastener, or arrange latch mechanism and to cooperatively interact locking.Lower floor is moveable drawer 3, and described drawer 3 is provided with hand grip portion, freely can take out and push away, and between the movable drawer structure 3 of casing 2 lower floor and casing 2, be provided with latch mechanism, after pushing described lower floor drawer 3, drawer 3 is stated in fastening lock residence.Described latch mechanism is any structure that can reach locking object, as being dual control snap close of the present invention.
Checkout equipment described in Fig. 1 comprises sample pre-treatments equipment, collaurum rapid detection card, experiment reagent, reading and data processing equipment and experiment consumptive material, the internal cavities that the levels of described casing 2 and case lid 1 are formed respectively is filled with the supporting mould of novel environment friendly plastics expanded material material, be provided with the consistent locating slot of same checkout equipment shape on the surface of described supporting mould, inlay respectively and hold described checkout equipment.Described sample pre-treatments equipment comprises that sample preparation is cut, sample preparation cutter, sample spoon, micro balance, pipettor, single track timer, vortex centrifugal all-in-one, tissue sample multiplication of voltage rapid treatment instrument, full Self-controllable level instruction nitrogen purge instrument; Described collaurum rapid detection card comprises sulfamido test card, quinolones test card, chloromycetin test card, gentamicin test card, Clenbuterol test card, Ractopamine test card, salbutamol test card; Described reading and data processing equipment comprise eight passage collaurum detector and work computer; Described experiment consumptive material comprises emgloves, centrifuge tube, accurate pH test paper, rifle head, dropper, lab-gown.
Preferably, the supporting mould of case lid described in Fig. 11 internal cavities is disposed with work computer locating slot 4 by order from left to right, from top to bottom, pipettor locating slot 5, sample preparation cutter locating slot 6, sample spoon locating slot 7, marking pen locating slot 8, sample preparation cut locating slot 9, micro balance locating slot 10 and timer locating slot 11; The supporting mould of described casing 2 upper strata internal cavities is disposed with collaurum rapid detection card locating slot 12, vortex centrifugal all-in-one locating slot 13, eight passage collaurum detector locating slot 14, Sample Grinder locating slot 15 by order from left to right, from top to bottom; In lower floor's drawer of described casing 2 internal cavities, supporting mould is disposed with full Self-controllable level instruction nitrogen by order from left to right, from top to bottom and purges instrument locating slot 16, tissue sample multiplication of voltage rapid treatment instrument locating slot 17, experiment consumptive material locating slot 18; Wherein, described multiple locating slot holds corresponding checkout equipment, and described operation instructions are placed in described work computer locating slot 4 together with work computer.
Device for detecting meat food safety of the present invention, wherein described in Fig. 1, the side of casing 2 also comprises wheel body (not shown), and opposite side is corresponding thereto provided with telescopic lever (not shown).Compact, conveniently moving, can move to rapidly destination and carry out Site Detection.Described wheel body is that 4 polyurethane increase the weight of roller, can bear larger weight, compact, conveniently moving, can move to rapidly destination and carry out Site Detection.Described detection case is made up of polypropylene composite material, and the eucaryotic cell structure of described polypropylene composite material, for opening nucleus, is engaged between each cell mutually, open nuclear structures design, lightweight, intensity is high, firm solid, another advantage effectively can absorb external force, therefore good damping effect.As shown in Figures 2 and 3, the casing 2 of described detection case or case lid 1 are also provided with multiple dual control snap close 20, preferably, 7 dual control snap closes 20 are evenly arranged on the edge of described case lid 1.Described dual control snap close 20 comprises hinged cramp 21 and fixture block 22, and the edge of described casing 2 is provided with the projection 24 adapted with described cramp 21, and described projection 24 is arranged on a circle of described casing 2 top; Described cramp 21 and described projection 24 fasten closes described detection case, close backward described casing 2 direction and promote described fixture block 22, fastening is closed more, described cramp 21 and fixture block 22 are in the on state as shown in Figure 3, under buckling state as shown in Figure 2, can open when there being soil, when having husky, shut, traditional design is difficult to shut as snap close enters dust; The side of described dual control snap close 20 and casing 2 corresponding thereto are also respectively arranged with the through hole 23 passed for lockset, and described casing 2 and case lid 1 are locked through described through hole 23 by lockset.Also be provided with O-ring seal at the edge of described case lid 1, obturation effect is good; Described detection case also comprises gas balance valve 19, and regulate inside and outside differential pressure, under plateau, high altitude environment, air can be entered, and hydrone can not be entered, moistureproof.
Device for detecting meat food safety of the present invention detects the application that in meat products, microbiotic, violated veterinary drug exceed standard at the scene fast.
Embodiment 2
Adopt the set of equipments of described field quick detection meat products safety to carry out the method detected, comprise the steps:
(1) first complete reading operation instructions before testing, and test card and sample to be checked are returned to room temperature; Collaurum rapid detection card is taken out from original packing bag;
(2) use sample extraction instrument, sampling, marking pen marks; Take 1-5g in centrifuge tube after organizing sample to pulverize, add sample extracting solution, with the mixing of vortex centrifugal all-in-one, the centrifugal 10-15min of 4000r/min, gets supernatant, to be checked;
Described extracting tool comprises that sample preparation is cut, sample preparation cutter, sample spoon and micro balance, Sample Grinder;
(3) collaurum rapid detection card is kept flat, draw the measuring samples solution after process with pipettor or dropper, vertically dropwise add 5-7 and drip in well, start after application of sample to use timer timing;
After (4) 5 minutes, test card is put into eight passage collaurum detectors and read data, carry out data processing with notebook computer.
Embodiment 3
For detecting a method for meat food safety, comprise the steps:
(1) first complete reading operation instructions before testing, and test card and sample to be checked are returned to room temperature; Clenobuterol hydrochloride test card is taken out from original packing bag;
(2) take fresh pork 2-5g with micro balance, put into tissue sample multiplication of voltage rapid treatment instrument and squeeze out sample liquids, with the centrifugal 10-15min of vortex centrifugal all-in-one 4000r/min, get supernatant, to be checked;
(3) described clenobuterol hydrochloride test card is kept flat, draw the measuring samples solution after process with pipettor or dropper, vertically dropwise add 5-7 and drip in well, after application of sample, start timing;
After (4) 5 minutes, test card is put into eight passage collaurum detectors and read data, carry out data processing with notebook computer.
Embodiment 4
For detecting its method of meat food safety, comprise the steps:
(1) first complete reading operation instructions before testing, and test card and sample to be checked are returned to room temperature; Chloromycetin test card is taken out from original packing bag;
(2) use sample extraction instrument, sampling, marking pen marks, and takes the pork sample after 2-5g pulverizing, adds sample extracting solution, with the mixing of vortex centrifugal all-in-one, and the centrifugal 10-15min of 4000r/min; Get supernatant 1mL in 10mL centrifuge tube, use full Self-controllable level to indicate nitrogen blow device about 50 DEG C evaporates to dryness; Add 500 μ L redissolution liquid, vortex oscillation, and with the continuous drawing wash of dropper, remain until dissolve tube wall completely; To be checked.
(3) described chloromycetin test card is kept flat, draw the measuring samples solution after process with pipettor or dropper, vertically dropwise add 5-7 and drip in well, after application of sample, start timing;
After (4) 5 minutes, test card is put into eight passage collaurum detectors and read data, carry out data processing with notebook computer.
Finally should be noted that: above embodiment is only in order to illustrate that technical scheme of the present invention is not intended to limit; although with reference to above-described embodiment to invention has been detailed description; those of ordinary skill in the field still can modify to the specific embodiment of the present invention or equivalent replacement; these do not depart from any amendment of spirit and scope of the invention or equivalent replacement, are all applying within the claims awaited the reply.
Claims (7)
1. one kind for detecting the device of meat food safety, it is characterized in that, described device comprises detection case and checkout equipment, it is inner that described checkout equipment is positioned at described detection case, the case lid that described detection case comprises double-layer box body and is flexibly connected with it, described checkout equipment comprises sample pre-treatments equipment, collaurum rapid detection card, experiment reagent, read and data processing equipment and test consumptive material, the side of described casing comprises wheel body, opposite side is corresponding thereto provided with telescopic lever, described wheel body is that 4 polyurethane increase the weight of roller, described casing is upper and lower double-layer structure, wherein lower floor is drawer-type structure, levels structure and the case lid inside of described casing form glove cavity respectively, wherein be filled with the supporting mould of novel environment friendly plastics expanded material material, be provided with the consistent locating slot of same checkout equipment shape on the surface of described supporting mould, inlay respectively and hold described checkout equipment.
2. the device for detecting meat food safety according to claim 1, it is characterized in that, described sample pre-treatments equipment comprises that sample preparation is cut, sample preparation cutter, marking pen, sample spoon, operation instructions, micro balance, pipettor, single track timer, vortex centrifugal all-in-one, tissue sample multiplication of voltage rapid treatment instrument, full Self-controllable level instruction nitrogen purges instrument;
Described collaurum rapid detection card comprises sulfamido test card, quinolones test card, chloromycetin test card, gentamicin test card, Clenbuterol test card, Ractopamine test card, salbutamol test card;
Described reading and data processing equipment comprise eight passage collaurum detector and work computer;
Described experiment consumptive material comprises emgloves, centrifuge tube, accurate pH test paper, rifle head, dropper, lab-gown.
3. the device for detecting meat food safety according to claim 1, is characterized in that, described detection case is made up of polypropylene composite material, and the eucaryotic cell structure of described polypropylene composite material, for opening nucleus, is engaged between each cell mutually; The casing of described detection case or case lid are also provided with multiple dual control snap close.
4. the device for detecting meat food safety according to claim 3, is characterized in that, described dual control snap close is 7, is evenly arranged on the edge of described case lid.
5. the device for detecting meat food safety according to claim 4, is characterized in that, is also provided with O-ring seal at the edge of described case lid; Described detection case also comprises gas balance valve.
6. the device for detecting meat food safety according to claim 2, it is characterized in that, the supporting mould of described case lid internal cavities is disposed with work computer locating slot by order from left to right, from top to bottom, pipettor locating slot, sample preparation cutter locating slot, sample spoon locating slot, marking pen locating slot, sample preparation cut locating slot, micro balance locating slot and timer locating slot;
The supporting mould of described box house upper strata cavity is disposed with collaurum rapid detection card locating slot, vortex centrifugal all-in-one locating slot, eight passage collaurum detector locating slots, Sample Grinder locating slot by order from left to right, from top to bottom; The supporting mould of lower floor's drawer-type structure internal cavity is disposed with full Self-controllable level instruction nitrogen by order from left to right, from top to bottom and purges instrument locating slot, tissue sample multiplication of voltage rapid treatment instrument locating slot, experiment consumptive material locating slot;
Wherein, described multiple locating slot holds corresponding checkout equipment, and described operation instructions are placed in described work computer locating slot together with work computer.
7. adopting a detection method for the device for detecting meat food safety in claim 1-6 described in any claim, it is characterized in that, comprise the steps:
1) test card and sample to be checked are returned to room temperature, then take out collaurum rapid detection card
2) use sample extraction instrument, sampling, marking pen marks; Take 2-5g in centrifuge tube after organizing sample to pulverize, add sample extracting solution, with the mixing of vortex centrifugal all-in-one, the centrifugal 10-15min of 4000r/min, gets supernatant, to be checked;
Described extracting tool comprises that sample preparation is cut, sample preparation cutter, sample spoon and micro balance, Sample Grinder;
3) collaurum rapid detection card is kept flat, draw the measuring samples solution after process with pipettor or dropper, vertically dropwise add 5-7 and drip in well, start after application of sample to use timer timing;
4) after 5 minutes, test card is put into eight passage collaurum detectors and read data, carry out data processing with notebook computer.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510303837.8A CN105044297B (en) | 2015-06-05 | 2015-06-05 | A kind of apparatus and method for detecting meat food safety |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510303837.8A CN105044297B (en) | 2015-06-05 | 2015-06-05 | A kind of apparatus and method for detecting meat food safety |
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| Publication Number | Publication Date |
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| CN105044297A true CN105044297A (en) | 2015-11-11 |
| CN105044297B CN105044297B (en) | 2017-05-31 |
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| CN201510303837.8A Expired - Fee Related CN105044297B (en) | 2015-06-05 | 2015-06-05 | A kind of apparatus and method for detecting meat food safety |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108982143A (en) * | 2018-06-25 | 2018-12-11 | 杭州富阳福士得食品有限公司 | A kind of food inspection extraction element with leakproofness |
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| CN115356484A (en) * | 2022-10-20 | 2022-11-18 | 黑龙江省农业科学院农产品质量安全研究所 | Complete equipment and detection method for rapidly detecting mycotoxin in grains |
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