CN105036945A - Culture medium for black fungus cultivation and preparation method thereof - Google Patents

Culture medium for black fungus cultivation and preparation method thereof Download PDF

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Publication number
CN105036945A
CN105036945A CN201510460887.7A CN201510460887A CN105036945A CN 105036945 A CN105036945 A CN 105036945A CN 201510460887 A CN201510460887 A CN 201510460887A CN 105036945 A CN105036945 A CN 105036945A
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China
Prior art keywords
parts
culture medium
preparation
black fungus
napier grass
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CN201510460887.7A
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唐小凤
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GUILIN HONGWANG MUSHROOM INDUSTRY Co Ltd
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GUILIN HONGWANG MUSHROOM INDUSTRY Co Ltd
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Publication of CN105036945A publication Critical patent/CN105036945A/en
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Abstract

The invention discloses a culture medium for black fungus cultivation and a preparation method thereof. The culture medium is prepared from the following raw materials in parts by weight: 50-60 parts of pennisetum purpureum schum, 35-45 parts of rice bran or wheat bran, 2-4 parts of bagasse, 0.5-1.5 parts of gypsum, 0.5-1.5 parts of quicklime and 0.05-0.15 part of common andrographis herb, and the ratio of materials to water is 1: 1.5. The culture medium is suitable for large-scale cultivation, and the obtained black fungus is rich in nutritional value and high in yield.

Description

Cloud ear culture medium for cultivating and preparation method thereof
Technical field
The present invention relates to field of edible fungus culture, particularly relate to cloud ear culture medium for cultivating and preparation method thereof.
Background technology
Cloud ear, i.e. black fungus, have another name called black dish, and mulberry ear, this bacterium, tree chicken, wooden moth, wood are fine and soft, and because of likeness in form ear, its color chocolate and gaining the name in addition, is the entity of the Basidiomycetes Auriculariaceae plant auricularia auriculajudae of Mycophyta, blazons what all over the world.Chang Jisheng what mulberry, Chinese scholartree, willow, open up, paper mulberry, all trees of elm.Black fungus is a kind of nutritious edible mushrooms, is again the traditional protective foods of China and exporting.Its another name is a lot, because being grown on rotten wood, and the ear of its likeness in form people, therefore named auricularia auriculajudae; Slim and graceful like moth butterfly again, have another name called wooden moth; Because its taste is delicious just like chicken, therefore also name sets chicken, wooden machine (it is machine that Gu Nanchu people calls chicken): the auricularia auriculajudae of polyphyll is inlayed mutually in the tree, just like blade floating clouds, has again the title of cloud ear.The auricularia auriculajudae that people often eat, mainly contains two kinds: a kind of is that the outside of belly is level and smooth, look black and back side crinosity gray or beige, claims Auricularia polytricha (Mout) Sacc. (being generally called wild auricularia auriculajudae); Another kind is that two sides is smooth, chocolate, translucent, be called brightness program.Auricularia polytricha (Mout) Sacc. is piece comparatively large, but quality is slightly tough, not easily chews, and taste is not good, cheap.Brightness program matter is soft delicious, slides and band is refreshing, nutritious, and being artificial a large amount of one of cultivating, is also the cloud ear that it has often been said.
Cloud ear, the flat taste of its property is sweet, people's stomach, large intestine channel.There is nourishing, moisturize, the effect of nourish blood beneficial stomach, promoting blood circulation and hemostasis, moistening lung, ease constipation.The nutritive ingredient of black fungus, according to modern science analysis, containing 10.6 grams, protein in every 100 grams of dry products, 0.2 gram, fat, 65 grams, carbohydrate, robust fibre 7 grams, calcium 375 milligrams, 201 milligrams, phosphorus, iron 185 milligrams, in addition containing vitaminB10 .15 milligram, Lin Suanna Vitamin B2 Sodium Phosphate 0.55 milligram, 2.7 milligrams, nicotinic acid.Therefore, black fungus always deeply by the liking of numerous people, during the Chang Zuowei cooking is various, the batching of western famous dish, or stew well, as Jiamei's dessert suitable for four seasons, not only clear and melodious deliciousness with red date, lotus seeds sugaring, sliding tender refreshing larynx, and have the effect increasing appetite and nourishing and fit keeping function.Black fungus has certain adsorptive power; Human body is had to the effect of washing clearly stomach and intestine and digest cellulose.Therefore, it is again textile worker, mine worker and the indispensable a kind of protective foods of barber.
Black fungus also can be done medicinal.Successive dynasties physician is documented in detail for the drug effect of black fungus, as Ming Dynasty's LI Shi-Zhen is recorded in Compendium of Material Medica: " auricularia auriculajudae is born on rotten wood, and property is sweet dry, cures mainly beneficial gas not hungry, strong will of making light of one's life by commiting suicide, and the effect such as blood under having treatment hemorrhoid, bloody flux." China's medical science has constantly held black fungus has moist strong, clearing lung-heat benefit gas, enriches blood and invigorates blood circulation, the effects such as sedation-analgesia.Be that the traditional Chinese medical science is used for treating lumbago and skelalgia, brothers knot numbness, the prescription drug that the illness such as bleeding hemorrhoids and puerperal asthenia is conventional.According to external, black fungus can lower blood clotting, relaxes coronary atherosclerosis, has special benefit to prevention and therapy coronary heart disease.Polysaccharose substance contained by black fungus, has certain antitumor action, it is reported that to the inhibiting rate of small white mouse sarcoma 180 be 42.6%.The among the people also useful black fungus of China adds and is decocted in water for oral dose, and treats woman cervix uteri cancer and carcinoma of vagina.
Cloud ear has good market outlook, and the cultivation technique of cloud ear is also being updated.
Summary of the invention
The object of this invention is to provide a kind of cloud ear culture medium for cultivating and preparation method thereof, be applicable to extensive cultivation, the cloud ear rich in nutritive value obtained, output is high.
The present invention is achieved by the following technical solution:
Cloud ear culture medium for cultivating and preparation method thereof,
Be made up of the raw material of following weight proportion: napier grass 50-60 part, rice bran or wheat bran 35-45 part, bagasse 2-4 part, gypsum 0.5-1.5 part, unslaked lime 0.5-1.5 part, Herba Andrographis 0.05-0.15 part, material-water ratio 1:1.5.
Preferably be made up of the raw material of following weight proportion: napier grass 55 parts, rice bran or 40 parts, wheat bran, bagasse 3 parts, 1 part, gypsum, unslaked lime 1 part, Herba Andrographis 0.1 part, material-water ratio 1:1.5.
Described bagasse preferably dries rear chopping or pulverizing; Rice bran or wheat bran can be arbitrary proportions.
The preparation method of substratum of the present invention:
1) pre-treatment: smashed by napier grass, puts into steam explosion tank, burstpressures 3.0-3.5Mpa, and the dwell time maintains 150-200s;
Herba Andrographis is cleaned, smashes, put into pressure pan, be forced into 20-30MPa, make its pressure uniform, and keep 10-20min, recover normal pressure;
2) by step 1) obtain material and the mixing of other raw materials, stir;
3) after adjustment mixing, the material-water ratio of material is 1:1.5, obtains substratum.
Compared with prior art, tool of the present invention has the following advantages:
1, napier grass has higher nutritive value, and in dry-matter, crude protein content reaches 16.52-18.06%, crude fat 2.1%, napier grass soft and succulency, and palatability is fine, digestibility and utilization ratio high, be generally use as feed.Utilize napier grass to make cloud ear culture medium for cultivating, can turn waste into wealth, extend industrial chain, improves agroecological environment, improves comprehensive benefit.
2, contain abundant fiber in napier grass, directly smash or pulverize, the nutritive value of napier grass can not make full use of.The present invention is by Steam explosion treatment napier grass, and the fiber in vegetable cell is bondd, under high temperature, high pressure steam effect by lignin, cellulose crystallity improves, and the polymerization degree declines, and hemicellulose fraction is degraded, lignin softens, horizontal strength of connection declines, even softening plastic, when being full of the material sudden pressure reduction of pressure steam, gas play in hole is expanded, produce " explosion " effect, partly can peel off lignin, and raw material is torn as fiber fines.By such process, the nutrition of napier grass can be fully utilized.
3, add Herba Andrographis in substratum of the present invention, there is the effect that removing toxic substances is antibacterial.Also can adding portion herbal medicine in prior art, be generally after smashing or pulverizing, or adopt the waste residue after plant extract activeconstituents, directly mix, such process, the alkaloid in Herba Andrographis can affect the antibacterial effect of removing toxic substances.The present invention is by autoclaving Herba Andrographis, and alkaloid is most all non-volatilities when normal pressure, the first melting of direct heating, continue and be decomposed, or melting and simultaneously decomposing, alkaloid under high pressure has distillation phenomenon, the present invention, by autoclaving, can destroy alkaloid.Fungistatic effect through the Herba Andrographis of process is like this more than the twice of prior art.
4, adopt substratum of the present invention to cultivate cloud ear, compared to existing technology, output can increase by more than 30%, and the cloud fungus polysaccharides contained in the cloud ear obtained can increase by more than 40%.
Embodiment
With embodiment, the invention will be further described below, but the present invention is not limited to these embodiments.
Embodiment 1:
Cloud ear culture medium for cultivating and preparation method thereof,
Be made up of the raw material of following weight proportion: napier grass 50 parts, rice bran or 45 parts, wheat bran, bagasse 2 parts, 1.5 parts, gypsum, unslaked lime 1.5 parts, Herba Andrographis 0.05 part, material-water ratio 1:1.5;
Preparation method:
1) pre-treatment: smashed by napier grass, puts into steam explosion tank, burstpressures 3.0-3.5Mpa, and the dwell time maintains 150-200s;
Herba Andrographis is cleaned, smashes, put into pressure pan, be forced into 30MPa, make its pressure uniform, and keep 20min, recover normal pressure;
2) by step 1) obtain material and the mixing of other raw materials, stir;
3) after adjustment mixing, the material-water ratio of material is 1:1.5, obtains substratum.
Embodiment 2:
Cloud ear culture medium for cultivating and preparation method thereof,
Be made up of the raw material of following weight proportion: napier grass 60 parts, rice bran or 35 parts, wheat bran, bagasse 4 parts, 0.5 part, gypsum, unslaked lime 0.5 part, Herba Andrographis 0.15 part, material-water ratio 1:1.5;
Preparation method:
1) pre-treatment: smashed by napier grass, puts into steam explosion tank, burstpressures 3.0-3.5Mpa, and the dwell time maintains 150-200s;
Herba Andrographis is cleaned, smashes, put into pressure pan, be forced into 25MPa, make its pressure uniform, and keep 10min, recover normal pressure;
2) by step 1) obtain material and the mixing of other raw materials, stir;
3) after adjustment mixing, the material-water ratio of material is 1:1.5, obtains substratum.
Embodiment 3:
Cloud ear culture medium for cultivating and preparation method thereof,
Be made up of the raw material of following weight proportion: napier grass 55 parts, rice bran or 40 parts, wheat bran, bagasse 3 parts, 1 part, gypsum, unslaked lime 1 part, Herba Andrographis 0.1 part, material-water ratio 1:1.5;
Preparation method:
1) pre-treatment: smashed by napier grass, puts into steam explosion tank, burstpressures 3.0-3.5Mpa, and the dwell time maintains 150-200s;
Herba Andrographis is cleaned, smashes, put into pressure pan, be forced into 20MPa, make its pressure uniform, and keep 15min, recover normal pressure;
2) by step 1) obtain material and the mixing of other raw materials, stir;
3) after adjustment mixing, the material-water ratio of material is 1:1.5, obtains substratum.

Claims (3)

1. the preparation method of cloud ear culture medium for cultivating, is characterized in that:
Be made up of the raw material of following weight proportion: napier grass 50-60 part, rice bran or wheat bran 35-45 part, bagasse 2-4 part, gypsum 0.5-1.5 part, unslaked lime 0.5-1.5 part, Herba Andrographis 0.05-0.15 part, material-water ratio 1:1.5;
Comprise the steps:
1) pre-treatment: smashed by napier grass, puts into steam explosion tank, burstpressures 3.0-3.5Mpa, and the dwell time maintains 150-200s;
Herba Andrographis is cleaned, smashes, put into pressure pan, be forced into 20-30MPa, make its pressure uniform, and keep 10-20min, recover normal pressure;
2) by step 1) obtain material and the mixing of other raw materials, stir;
3) after adjustment mixing, the material-water ratio of material is 1:1.5, obtains substratum.
2. the preparation method of cloud ear culture medium for cultivating according to claim 1, is characterized in that: be made up of the raw material of following weight proportion: napier grass 55 parts, rice bran or 40 parts, wheat bran, bagasse 3 parts, 1 part, gypsum, unslaked lime 1 part, Herba Andrographis 0.1 part, material-water ratio 1:1.5.
3. the cloud ear culture medium for cultivating that obtains of preparation method according to claim 1 and 2.
CN201510460887.7A 2015-07-31 2015-07-31 Culture medium for black fungus cultivation and preparation method thereof Pending CN105036945A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107285877A (en) * 2017-07-14 2017-10-24 广西科学院生物研究所 Culture medium, cultural method and the bacterium rod grand style structure cultivated for open field cloud ear

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101699967A (en) * 2009-12-01 2010-05-05 福建农大菌草技术开发公司 Method for cultivating edible fungi and medicinal fungi by utilizing fresh fungi grass
CN104547211A (en) * 2014-12-30 2015-04-29 广西百利乐生物科技有限公司 Anti-carsickness drink

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101699967A (en) * 2009-12-01 2010-05-05 福建农大菌草技术开发公司 Method for cultivating edible fungi and medicinal fungi by utilizing fresh fungi grass
CN104547211A (en) * 2014-12-30 2015-04-29 广西百利乐生物科技有限公司 Anti-carsickness drink

Non-Patent Citations (3)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107285877A (en) * 2017-07-14 2017-10-24 广西科学院生物研究所 Culture medium, cultural method and the bacterium rod grand style structure cultivated for open field cloud ear

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Application publication date: 20151111