CN105030855B - A kind of prevention and cure of cardiovascular disease, hyperlipemia health protection tea and preparation method thereof and detection method - Google Patents
A kind of prevention and cure of cardiovascular disease, hyperlipemia health protection tea and preparation method thereof and detection method Download PDFInfo
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Abstract
The present invention provides a kind of prevention and cure of cardiovascular disease, hyperlipemia health protection tea and preparation method thereof and detection methods, the health protection tea is made of 200 800 parts of ginkgo biloba p.e, 700 1300 parts of Ilex Latifolia Thunb of raw material, health protection tea is prepared using the method that ginkgo biloba p.e is mixed with Ilex Latifolia Thunb after fermented processing, its beneficiating ingredient is not only fully used, but also the performance and effect of ginkgo leaf, Ilex Latifolia Thunb can be fully retained；And fermented treated ginkgo Ilex Latifolia Thunb, rich in there are many amino acid and vitamins, the medical curative effect of ginkgo bitter leaves health protection tea obtained is significantly improved compared with the prior art, and excellent taste.In addition detection method of the invention energy is easy, is quickly and efficiently detected to health protection tea, ensure that product quality.
Description
Technical field
The present invention relates to technical field of traditional Chinese medicines, more particularly to a kind of prevention and cure of cardiovascular disease, hyperlipemia health protection tea and
Preparation method and detection method.
Background technology
Ginkgo is China specialty plant.Yield accounts for global 70%.Whole body is all precious, and seed, root, leaf can be made
It is medicinal and curative for effect reliable.The medical value of ginkgo is long, and effect is most non-irrigated to be recorded in《Sheng Nong's herbal classic》, have moistening lung, put down
Asthma, cough-relieving and other effects.《Compendium of Materia Medica》Also on the books " its nature and flavor sweetness and bitterness and it is puckery, enter lung, kidney two warp, there is relieving asthma and cough, stop-band
It is turbid, reduce just ", the records such as " treating stranguria effect ".
The exploitation of Ginkgo leaf preparation starts from Europe, global first ginkgo of nineteen sixty-five Germany Shi Wabai pharmacy group listing
Leaf preparation " ladder is protected peaceful " (Tebonin) then has the Tanakan of rich one Yi Pusheng drugmakers of the good fortune production of France.German, France,
The country such as the U.S., Japan, Italy develops the various preparations extracted with ginkgo leaf in succession, and for treating cardiovascular and cerebrovascular disease
And senile dementia etc., become the situation of selling well drug in European disease of old people medication.
It is counted according to ginkgo leaf association of the world, the world wide annual sales amount in ginkgo biloba p.e correlated product market is broken through
50000000000 yuan, and with annual 60~80% speed increase.World's ginkgo leaf upsurge is just in the ascendant.Nearly 20 years, entire generation
Boundary's especially western developed country starts a burst of green waves, and cause is being widely used due to synthetic drug such as antibiotic,
The especially abuse of such drug, the misuse injury caused by health, makes medical field form a kind of common recognition, that is, comes from green
Often there is the product of life (plant) larger safety, exploitation hot spot of the ginkgo as modern age autonomic drug to be in green
The forward position of tide.
Ilex Latifolia Thunb, making drug and drink use in Guizhou minority nationality regions has long history, one word of Ilex Latifolia Thunb beginning to see
In Eastern Han Dynasty's (25~225 years Christian eras), until the Qing Dynasty is classified as imperial palace tribute as rare specialty.It is investigated according to expert, ancient literature
Signified highland Lu or Ilex Latifolia Thunb is the Cortex Ilicis Rotundae tree of Aquifoliaceae.In April, 1958《The civil prescription collection in Guizhou》Head carries Guizhou bitter leaves
Tea, also known as japanese ligusticum leaf make tealeaves substitute and hyoscine with branches and leaves Ilex Latifolia Thunb, civil as refrigerant antipyretic, diuretics, but
Its uncertain plant origin.Book the 3rd edition in 1978 record " Ilex Latifolia Thunb, also known as japanese ligusticum leaf, herb mixtures tea cream, bitter taste dissipate, make freshener,
There are antipyretic refrigerant, diuresis, antihypertensive effect, it is swollen to control furunculosis poison outside ".Zhang Jingjie etc. has found that Ilex Latifolia Thunb [Ligustrum robust is produced in Guizhou Province
L.robustum (Roxb.) BL.] leaf alcohol extract and decocting liquid can reduce Rat Experimental hyperlipidemia, and have certain analgesia
Effect and the effect for reducing anaesthetized dog blood pressure.The general flavone and decocting liquid of the report Ilex Latifolia Thunb such as Huang Linfang (Ligustrum robust) are to nutrition
Property obesity have a preventive and therapeutic action, the volatile oil and decocting liquid of Ilex latifolia tea-leaf can significantly inhibit mice ear and reduce mouse
Abdominal cavity capillary permeability, it was demonstrated that Ilex Latifolia Thunb volatile oil and decocting liquid have good anti-inflammatory effect.Wang Qichun etc. uses Guizhou
The extracted extract piece for the Ilex latifolia tea-leaf that the south of Guizhou Province locality Miao ethnic group, cotton clothes race use, treats essential hypertension and cardiovascular and cerebrovascular disease is total
200, it is found that Ilex Latifolia Thunb can treat dizzy, headache caused by above-mentioned disease, uncomfortable in chest, weak, insomnia, can reduce blood pressure, adjust
Blood fat improves hemorheology status.
Single ginkgo leave tea or ginkgo leaf in the prior art and tealeaves assemble and ginkgo leaf with other beneficial to raw material group
With the health protection tea that object is prepared into, generally existing has ignored the utilizing status of effective component of ginkgo leaf, as a result leads to its product effect
Difference is extremely difficult to anticipation effect, to affect the cognition degree of consumer, it is difficult to promote.
Such as：Patent No. CN201210039384.9, patent name " a kind of ginkgo Ilex Latifolia Thunb and preparation method thereof "；And specially
Profit CN201210104586.7, patent name " a kind of ginkgo leaf extraction process and the processing method of ginkgo Ilex Latifolia Thunb ",
Ginkgo leaf is mixed with Ilex Latifolia Thunb and directly enters tea, and effective component of ginkgo leaf extract dosage is not high, drug effect dissolution rate is not high,
Processing technology is unreasonable, is only simple method for preparing tea, is extremely difficult to the medical curative effect of ginkgo leaf, Ilex Latifolia Thunb, can not achieve existing
There is the medical value of ginkgo Ilex Latifolia Thunb.
Invention content
The present invention for overcome the deficiencies in the prior art, by a large amount of prescription screening and verification, provides a kind of prevention
Health protection tea of angiocardiopathy, hyperlipemia and preparation method thereof and detection method, beneficiating ingredient are not only fully used,
And the performance and effect of ginkgo leaf, Ilex Latifolia Thunb can be fully retained, and ginkgo bitter leaves health protection tea obtained is also rich in largely
Amino acid and vitamin, medical curative effect is significantly improved compared with the prior art, and excellent taste.
Specifically, the present invention provides the following technical solutions：
A kind of prevention and cure of cardiovascular disease, hyperlipemia health protection tea, it is characterised in that：It calculates, is carried by ginkgo leaf by weight
200-800 parts of object, 700-1300 parts of Ilex Latifolia Thunb is taken to be made of raw material.
Further, it calculates, is made of raw material of 500 parts of ginkgo biloba p.e, 1000 parts of Ilex Latifolia Thunb by weight.
Preparation method includes the following steps：
(1) it takes formula ratio Ilex Latifolia Thunb to clean or clean, crushes, soaked with the ethyl alcohol of 20-30%, by former per 1kg Ilex Latifolia Thunbs
5-10g probiotics leavens, sealing is added in material, and room temperature is fermented 40-60 hours, is taken out, dry, and the Ilex Latifolia Thunb to be fermented is former
Material；
(2) frying pot to be opened, stirring rod is adjusted, is put into the Ilex Latifolia Thunb that fermentation is made in step 1, temperature control is 60-70 DEG C,
After stir-frying 10-20 minutes, the ginkgo biloba p.e of the amount is taken, hydrojet is made with water, uniformly sprayed into frying pot, wait for uniformly mixing
Afterwards, mixture is put into sterilizing, drying equipment, first high-temperature sterilization, is then cooled to 80 DEG C rapidly hereinafter, low temperature drying, crosses 80
Mesh sieve, packing to get.
Further, preparation method includes the following steps：
(1) it takes the amount Ilex Latifolia Thunb to clean or clean, crushes, soaked with 25% ethyl alcohol, added by every 1kg Ilex Latifolia Thunbs raw material
Enter 6g probiotics leavens, seal, room temperature is fermented 50 hours, is taken out, dry, the Ilex Latifolia Thunb raw material to be fermented；
(2) frying pot to be opened, stirring rod is adjusted, is put into the Ilex Latifolia Thunb that fermentation is made in step 1, temperature control is 60-70 DEG C,
After stir-frying 15 minutes, the ginkgo biloba p.e of the amount is taken, hydrojet is made with water, uniformly sprayed into frying pot, wait for after evenly mixing,
Mixture is put into sterilizing, drying equipment, first high-temperature sterilization, is then cooled to 80 DEG C rapidly hereinafter, low temperature drying, crosses 80 mesh
Sieve whole grain, packing to get.
The ginkgo biloba p.e is cleaned or is cleaned by ginkgo leaf, is crushed, and 80% ethyl alcohol, leaching are measured in 5-8 times of addition
2h is steeped, heating and refluxing extraction 3-4 hours, filtering, filtrate recycling ethanol to medicinal extract is dry, obtains ginkgo leaf ethanol extract；Medicine
Slag adds the 5-8 times of water measured, water to decoct 2-3h, and filtering, filtrate concentration is dry, obtains ginkgo leaf water extract, merges ginkgo leaf second
Alcohol extracting thing and ginkgo leaf water extract are to get to ginkgo biloba p.e.
The present invention also provides above-mentioned prevention and cure of cardiovascular disease, the detection method of hyperlipemia health protection tea, including following steps
Suddenly：
Character：Product is to bagged instant preparation, and content is the powder or particle of sepia；Gas is micro-, mildly bitter flavor Hui Tian；
Differentiate：(1) this product 2g is taken, it is finely ground, add n-butanol 10-20ml, sets water-bath medium temperature and soak 10-20 minutes and constantly shake
It shakes, lets cool, filter, filtrate is evaporated, and residue adds ethyl alcohol 2ml to make dissolving, as test solution；Separately take ginkgo leaf reference extract
1g is made in the same way of reference extract solution；It is tested according to thin-layered chromatography, draws each 3 μ l of above two solution, put respectively in same
Using the carboxymethylcellulose sodium solution containing 4% sodium acetate as on the silica gel g thin-layer plate of binder, with ethyl acetate-butanone-first
Alcohol-water (5：3：1：1) it is solvent, is unfolded, take out, dry, sprays with 3% alchlor ethanol solution, set daylight and purple respectively
It is inspected under outer smooth lamp (365nm).In test sample chromatography, show identical on position corresponding with reference extract chromatography, under daylight
The spot of color shows the fluorescence spot of same color under ultraviolet light；
(2) this product 2g is taken, adds ethyl acetate 20-30ml, water-bath to flow back 30 minutes, filtration, filtrate is evaporated, and residue adds ethyl alcohol
5ml makes dissolving, as test solution；Ilex Latifolia Thunb control medicinal material 2g separately is taken, is made in the same way of control medicinal material solution；According to thin-layer chromatography
Method is tested, and each 10 μ l of above two solution (band point sample) are drawn, and is put respectively on same silica G plate, with ethyl acetate-fourth
Ketone-formic acid-water (10:3:1:1) it is solvent, is unfolded, take out, dry, sprays 5% ferric trichloride ethanol solution being acidified with hydrochloric acid
(taking ferric trichloride 5g, add ethyl alcohol to dissolve and be diluted to 100ml, add hydrochloric acid 1ml), 100 DEG C to be heated to spot development clear, for examination
In product chromatography, on control medicinal material chromatography corresponding position, the spot of same color is shown.
It checks：By being checked under bagged instant preparation item.
Beneficial effects of the present invention are：Ginkgo leaf is mixed with Ilex Latifolia Thunb the present invention overcomes the prior art and directly enters tea,
Its drug effect is invigorated blood circulation the not high disadvantage of dissolution rate, by the research again matched to ginkgo Ilex Latifolia Thunb prescription and optimization processing technology,
Ginkgo biloba p.e and Ilex Latifolia Thunb after fermented processing are mixed with, beneficiating ingredient is not only fully used, and
And the performance and effect of ginkgo leaf, Ilex Latifolia Thunb can be fully retained；And it has been surprisingly found that fermented treated ginkgo bitter leaves
Tea, rich in there are many amino acid and vitamin, the medical curative effect of ginkgo bitter leaves health protection tea obtained is significantly carried compared with the prior art
Height, and excellent taste.In addition detection method of the invention energy is easy, is quickly and efficiently detected to health protection tea, ensure that
Product quality.
Test example：
(1) technical study：
Ginkgo Ilex Latifolia Thunb, prior art processing technology is relatively simple, such as patent No. CN201210039384.9, patent name
" a kind of ginkgo Ilex Latifolia Thunb and preparation method thereof "；And patent No. CN201210104586.7, " a kind of ginkgo leaf carries patent name
The processing method of taking technique and ginkgo Ilex Latifolia Thunb ", will treated that ginkgo leaf mixes with Ilex Latifolia Thunb directly enters tea, and ginkgo
The dosage of extract is few in leaf, therefore its mouthfeel and medical curative effect are not satisfactory；Inventors believe that directly by ginkgo leaf with
Ilex Latifolia Thunb is directly used as medicine, and impurity is more, and the dissolution of hot water Instant Drinks active constituent is low, and in addition Ilex Latifolia Thunb is without rationally handling its prophyll hardship
Astringent taste and pungent taste are heavier, and mouthfeel is bad；The present inventor, which studies, to be found：Leavening is inoculated into Ilex Latifolia Thunb raw material, in certain condition
Lower breeding, metabolite can make fermentation Ilex Latifolia Thunb have the characteristics such as certain acidity, flavour, fragrance, product made to increase preservation
Time, while nutritive value and digestibility can be improved；By the understanding to the prior art, following contrast test is made：
Test group 1：Ginkgo leaf is cleaned with Ilex Latifolia Thunb, is pulverized and mixed, and is dispensed；
Test group 2：It is prepared by method disclosed in patent No. CN201210039384.9；
Test group 3：It is prepared by method disclosed in patent No. CN201210104586.7；
Test group 4：It is prepared by the method for the embodiment of the present invention 3；
Health protection tea made of the above method carries out brewed in hot water, and quality investigation, wherein result such as the following table 1 are carried out to tea liquid：
Table 1：Ginkgo Ilex Latifolia Thunb quality made of Different Preparation is investigated
Test result shows：Preparation process of the present invention is more reasonable compared with prior art processes, manufactured health protection tea activity
Ingredient dissolution is high, is rich in a variety of amino acid and vitamin, and medical effect is more preferably and unique in taste, splendid.
(2) preparation technology parameter of the present invention is investigated：
Influencing health care tea qualitative factor of the present invention predominantly influences kudingcha fermented parameter：Concentration of alcohol, leavening
Dosage, fermentation time etc., therefore the present invention also have passed through a large amount of kudingcha fermented technological parameter screening test, part test
As a result such as the following table 2：
Table 2：Present invention process parameter is investigated：
It is above-mentioned experiments have shown that：By the investigation to technological parameter, concentration of alcohol is too low and leavening dosage is too low, when finding
Between it is too short, kudingcha fermented undesirable, active constituent dissolution is low, ginkgo Ilex Latifolia Thunb poor taste is made, when concentration of alcohol and hair
Ferment agent dosage is excessively high, fermentation time is long, and kudingcha fermented degree is excessively high, loses Ilex Latifolia Thunb primary characteristic, alcohol taste compared with
Dense, mouthfeel is poor, and therefore, the preferably kudingcha fermented technological parameter of the present invention is：20-30% ethyl alcohol, leavening dosage are 5-
8g/1kg raw materials, fermentation time 40-60h.
(3) pharmacodynamics is investigated：
1, to the influence of hyperlipemia mice
Mouse 90 is taken, male and female have concurrently, are randomly divided into 8 groups, every group 10.After fasting 16h：
Low constituent test group：Liquid 2g/kg (i.e. 0.1ml/10g) made from gavage embodiment 1；
Test group 1：Liquid 2g/kg (i.e. 0.1ml/10g) made from the gavage embodiment of the present invention 2；
Test group 2：Liquid 2g/kg (i.e. 0.1ml/10g) made from the gavage embodiment of the present invention 3；
Test group 3：Liquid 2g/kg (i.e. 0.1ml/10g) made from the gavage embodiment of the present invention 4；
High constituent test group：Liquid 2g/kg (i.e. 0.1ml/10g) made from the gavage embodiment of the present invention 5；
Control drug group 1：The liquid 0.1ml/10g that gavage ginkgo leaf is directly pulverized and mixed with Ilex Latifolia Thunb；
Control drug group 2：Liquid 0.1ml/10g made of gavage patent CN201210039384.9 methods；It is right
According to medicine group 3：Liquid 0.1ml/10g made of gavage patent CN201210104586.7 methods；
Normal group and control group high in fat feed distilled water 0.1ml/ only.Equal abdominal cavity is noted in addition to Normal group within 1 hour after administration
Penetrate 75% fresh -laid egg lotion 0.5ml/ only.Eyeball blood is taken to survey serum cholesterol after 20 hours.As a result 3 be see the table below.
Table 3：Influence (X- ± s) to induced Hyperlipidemia in Mice
| Group | Dosage (ml/10g) | Serum cholesterol |
| Normal group | -- | 3.75±0.51 |
| High in fat group | -- | 14.64±3.26Δ |
| Low group of grouping | 0.1 | 10.82±2.17* |
| Test group 1 | 0.1 | 6.13±2.31* |
| Test group 2 | 0.1 | 5.33±2.13* |
| Test group 3 | 0.1 | 6.21±2.34* |
| High constituent test group | 0.1 | 11.01±3.12* |
| Control group 1 | 0.1 | 10.15±2.68* |
| Control group 2 | 0.1 | 9.32±2.43* |
| Control group 3 | 0.1 | 8.86±2.35* |
Note：Compared with normal group, Δ P ＜ 0.01；The * P ＜ 0.05 compared with high in fat group.
4.2.2 to the influence of rat acute renal hypertension
Male rat 20 is taken, drug and dosage shown according to the form below are grouped administration at random, daily gastric infusion 1 time, continuously
14 days, after last dose 30min, the left kidney of each mouse is detached under etherization, with silver brain clip clamp kidney base of a fruit 4-5h.Then it is injected intraperitoneally
Yellow Jackets 40mg/kg anesthesia, detaches the total artery of strength, is exerted all one's strength arterial cannulation with the plastic pipe containing 0.1% heparin-saline,
It is parallel on mercury voltage detector and traces blood pressure.Silver brain clip is taken out after blood pressure is steady, rat blood pressure rises, and tends to level values after five minutes,
Compare each group rat and puts the front and back blood pressure of folder.As a result it see the table below, each group rat blood pressure of the present invention, which increases, is less than control drug group,
Illustrate the product that this method obtains, has the function of antagonism acut renal hypertension, and effect is better than control sample.
Influence (X- ± s) of the table 4 to rat acute renal hypertension
Above-mentioned experiment shows that ginkgo Ilex Latifolia Thunb prepared by the production method using 2-4 of the embodiment of the present invention can be substantially reduced
The blood fat of mouse high in fat has obvious inhibiting effect to rat acute renal hypertension, and function and effect are bitter better than existing ginkgo
Fourth tea, and embodiment 1 and embodiment 5 are respectively low component and high group grouping, drug action is substantially reduced, is not achieved and effectively controls
It treats, therefore optimizing prescriptions of the present invention are：200-800 parts of ginkgo biloba p.e, 700-1300 parts of Ilex Latifolia Thunb are made of raw material.
Specific implementation mode：
Embodiment 1：The preparation of low component health protection tea：
(1) it takes 600g Ilex Latifolia Thunbs to clean or removal of impurities, crushing is soaked with 20% ethyl alcohol, addition 5g probiotics leavens, it is close
Envelope, room temperature are fermented 40 hours, are taken out, dry, the Ilex Latifolia Thunb raw material to be fermented.
(2) frying pot is opened, stirring rod is adjusted, is put into the Ilex Latifolia Thunb raw material that fermentation is made in step 1, temperature control is 60-
70 DEG C, stir-frying after ten minutes, takes the ginkgo biloba p.e of 180g, hydrojet is made with water, uniformly sprays into frying pot, waits for uniformly mixing
Afterwards, mixture is put into sterilizing, drying equipment, first high-temperature sterilization, is then cooled to 80 DEG C rapidly hereinafter, low temperature drying, crosses 80
Mesh sieve, packing, is made 1000 bags of bagged teas.
Embodiment 2：The preparation of health protection tea：
(1) it takes 700g Ilex Latifolia Thunbs to clean or removal of impurities, crushing is soaked with 20% ethyl alcohol, addition 5g probiotics leavens, it is close
Envelope, room temperature are fermented 40 hours, are taken out, dry, the Ilex Latifolia Thunb raw material to be fermented.
(2) frying pot is opened, stirring rod is adjusted, is put into the Ilex Latifolia Thunb raw material that fermentation is made in step 1, temperature control is 60-
70 DEG C, stir-frying after ten minutes, takes the ginkgo biloba p.e of 200g, hydrojet is made with water, uniformly sprays into frying pot, waits for uniformly mixing
Afterwards, mixture is put into sterilizing, drying equipment, first high-temperature sterilization, is then cooled to 80 DEG C rapidly hereinafter, low temperature drying, crosses 80
Mesh sieve, packing, is made 1000 bags of bagged teas.
Embodiment 3：The preparation of health protection tea：
(1) it takes 1000g Ilex Latifolia Thunbs to clean or removal of impurities, crushing is soaked with 20% ethyl alcohol, addition 6g probiotics leavens,
Sealing, room temperature are fermented 50 hours, are taken out, dry, the Ilex Latifolia Thunb raw material to be fermented.
(2) frying pot is opened, stirring rod is adjusted, is put into the Ilex Latifolia Thunb raw material that fermentation is made in step 1, temperature control is 60-
70 DEG C, stir-frying after twenty minutes, takes the ginkgo biloba p.e of 500g, hydrojet is made with water, uniformly sprays into frying pot, waits for uniformly mixing
Afterwards, mixture is put into sterilizing, drying equipment, first high-temperature sterilization, is then cooled to 80 DEG C rapidly hereinafter, low temperature drying, crosses 80
Mesh sieve, packing, is made 1000 bags of bagged teas.
Embodiment 4：The preparation of health protection tea：
(1) it takes 1300g Ilex Latifolia Thunbs to clean or removal of impurities, crushing is soaked with 20% ethyl alcohol, addition 8g probiotics leavens,
Sealing, room temperature are fermented 60 hours, are taken out, dry, the Ilex Latifolia Thunb raw material to be fermented.
(2) frying pot is opened, stirring rod is adjusted, is put into the Ilex Latifolia Thunb raw material that fermentation is made in step 1, temperature control is 60-
70 DEG C, stir-frying after twenty minutes, takes the ginkgo biloba p.e of 800g, hydrojet is made with water, uniformly sprays into frying pot, waits for uniformly mixing
Afterwards, mixture is put into sterilizing, drying equipment, first high-temperature sterilization, is then cooled to 80 DEG C rapidly hereinafter, low temperature drying, crosses 80
Mesh sieve, packing, is made 1000 bags of bagged teas.
Embodiment 5：The preparation of high component health protection tea：
(1) it takes 1400g Ilex Latifolia Thunbs to clean or removal of impurities, crushing is soaked with 20% ethyl alcohol, addition 5g probiotics leavens,
Sealing, room temperature are fermented 40 hours, are taken out, dry, the Ilex Latifolia Thunb raw material to be fermented.
(2) frying pot is opened, stirring rod is adjusted, is put into the Ilex Latifolia Thunb raw material that fermentation is made in step 1, temperature control is 60-
70 DEG C, stir-frying after ten minutes, takes the ginkgo biloba p.e of 900g, hydrojet is made with water, uniformly sprays into frying pot, waits for uniformly mixing
Afterwards, mixture is put into sterilizing, drying equipment, first high-temperature sterilization, is then cooled to 80 DEG C rapidly hereinafter, low temperature drying, crosses 80
Mesh sieve, packing, is made 1000 bags of bagged teas.
Embodiment 6：Health protection tea quality testing
Character：Product is bagged instant preparation, and content is the powder or particle of sepia；Gas is micro-, mildly bitter flavor Hui Tian；
Differentiate：(1) health protection tea 2g is taken, it is finely ground, add n-butanol 15ml, sets water-bath medium temperature and soak 15 minutes and constantly shake, put
Cold, filtration, filtrate is evaporated, and residue adds ethyl alcohol 2ml to make dissolving, as test solution.Ginkgo leaf reference extract 1g separately is taken, together
Legal system is in pairs according to extract solution.It is tested according to thin-layered chromatography, draws each 3 μ l of above two solution, put respectively in same to contain
The carboxymethylcellulose sodium solution of 4% sodium acetate is on the silica gel g thin-layer plate of binder, with ethyl acetate-butanone-methanol-water
(5：3：1：1) it is solvent, is unfolded, take out, dry, sprays with 3% alchlor ethanol solution, set daylight and ultraviolet lamp respectively
It is inspected under (365nm).In test sample chromatography, on position corresponding with reference extract chromatography, same color is shown under daylight
Spot shows the fluorescence spot of same color under ultraviolet light.
(2) health protection tea 2g is taken, adds ethyl acetate 25ml, water-bath to flow back 30 minutes, filtration, filtrate is evaporated, and residue adds ethyl alcohol
5ml makes dissolving, as test solution；Ilex Latifolia Thunb control medicinal material 2g separately is taken, is made in the same way of control medicinal material solution；According to thin-layer chromatography
Method is tested, and each 10 μ l of above two solution (band point sample) are drawn, and is put respectively on same silica G plate, with ethyl acetate-fourth
Ketone-formic acid-water (10:3:1:1) it is solvent, is unfolded, take out, dry, sprays 5% ferric trichloride ethanol solution being acidified with hydrochloric acid
(taking ferric trichloride 5g, add ethyl alcohol to dissolve and be diluted to 100ml, add hydrochloric acid 1ml), 100 DEG C to be heated to spot development clear, for examination
In product chromatography, on control medicinal material chromatography corresponding position, the spot of same color is shown.
It checks：By being checked under bagged instant preparation item.
Testing result is shown：Product characteristics that embodiment 1-3 is prepared, discriminating, the scope of examination meet the requirements.
Claims (5)
1. a kind of health protection tea of prevention and cure of cardiovascular disease, it is characterised in that：It calculates by weight, by ginkgo biloba p.e 200-
800 parts, 700-1300 parts of Ilex Latifolia Thunb is made as follows for raw material：
(1) it takes formula ratio Ilex Latifolia Thunb to clean or clean, crushes, soaked with the ethyl alcohol of 20-30%, added by every 1kg Ilex Latifolia Thunbs raw material
Enter 5-10g probiotics leavens, seal, room temperature is fermented 40-60 hours, is taken out, dry, the Ilex Latifolia Thunb raw material to be fermented；
(2) frying pot is opened, stirring rod is adjusted, is put into the Ilex Latifolia Thunb that fermentation is made in step 1, temperature control is 60-70 DEG C, stir-frying
After 10-20 minutes, the ginkgo biloba p.e of the amount is taken, hydrojet is made with water, frying pot is uniformly sprayed into, waits for after evenly mixing,
Mixture is put into sterilizing, drying equipment, first high-temperature sterilization, is then cooled to 80 DEG C rapidly hereinafter, low temperature drying, crosses 80 mesh
Sieve whole grain, packing to get.
2. the health protection tea of prevention and cure of cardiovascular disease as described in claim 1, it is characterised in that：It calculates by weight, by ginkgo
500 parts of leaf extract, 1000 parts of Ilex Latifolia Thunb are made of raw material.
3. the health protection tea of prevention and cure of cardiovascular disease as claimed in claim 1 or 2, it is characterised in that：Preparation method includes following
Step：
(1) it takes the amount Ilex Latifolia Thunb to clean or clean, crushes, soaked with 25% ethyl alcohol, 6g is added by per 1kg Ilex Latifolia Thunb raw materials
Probiotics leaven, sealing, room temperature are fermented 50 hours, are taken out, dry, the Ilex Latifolia Thunb raw material to be fermented；
(2) frying pot is opened, stirring rod is adjusted, is put into the Ilex Latifolia Thunb that fermentation is made in step 1, temperature control is 60-70 DEG C, stir-frying
After 15 minutes, the ginkgo biloba p.e of the amount is taken, hydrojet is made with water, frying pot is uniformly sprayed into, waits for after evenly mixing, will mix
It closes object to be put into sterilizing, drying equipment, first high-temperature sterilization, is then cooled to 80 DEG C rapidly hereinafter, low temperature drying, it is whole to cross 80 mesh sieve
Grain, packing to get.
4. the health protection tea of prevention and cure of cardiovascular disease as claimed in claim 1 or 2, it is characterised in that：The ginkgo leaf extraction
Object is cleaned or is cleaned by ginkgo leaf, crush, be added 5-8 times measure 80% ethyl alcohol, impregnate 2h, heating and refluxing extraction 3-4 hours,
Filtering, filtrate recycling ethanol to medicinal extract is dry, obtains ginkgo leaf ethanol extract；The dregs of a decoction add the 5-8 times of water measured, water to decoct 2-
3h, filtering, filtrate concentration is dry, obtains ginkgo leaf water extract, merges ginkgo leaf ethanol extract and is extracted with ginkgo leaf water
Object is to get to ginkgo biloba p.e.
5. the health protection tea of prevention and cure of cardiovascular disease as claimed in claim 1 or 2, it is characterised in that：Detection method includes following
Step：
Character：Product is bagged instant preparation, and content is the powder or particle of sepia；Gas is micro-, mildly bitter flavor Hui Tian；
Differentiate：(1) health protection tea 2g is taken, it is finely ground, add n-butanol 10-20ml, sets water-bath medium temperature and soak 10-20 minutes and constantly shake,
It lets cool, filters, filtrate is evaporated, and residue adds ethyl alcohol 2ml to make dissolving, as test solution；Ginkgo leaf reference extract 1g separately is taken,
It is made in the same way of reference extract solution；According to thin-layered chromatography test, draw each 3 μ l of above two solution, put respectively in it is same with
Carboxymethylcellulose sodium solution containing 4% sodium acetate is on the silica gel g thin-layer plate of binder, with ethyl acetate：Butanone：Methanol：
Water=5：3：1：1 is solvent, is unfolded, and takes out, dries, and sprays with 3% alchlor ethanol solution, sets daylight respectively and wavelength is
It is inspected under the ultraviolet lamp of 365nm；In test sample chromatography, on position corresponding with reference extract chromatography, phase is shown under daylight
With the spot of color, the fluorescence spot of same color is shown under ultraviolet light；
(2) health protection tea 2g is taken, adds ethyl acetate 20-30ml, water-bath to flow back 30 minutes, filtration, filtrate is evaporated, and residue adds ethyl alcohol
5ml makes dissolving, as test solution；Ilex Latifolia Thunb control medicinal material 2g separately is taken, is made in the same way of control medicinal material solution；According to thin-layer chromatography
Method is tested, and each 10 μ l of above two solution are drawn, and band point sample is put respectively on same silica G plate, with ethyl acetate：Butanone：
Formic acid：Water=10:3:1:1 is solvent, is unfolded, and takes out, dries, and sprays 5% ferric trichloride ethanol solution being acidified with hydrochloric acid,
100 DEG C to be heated to spot development clear, in test sample chromatography, on control medicinal material chromatography corresponding position, shows same color
Spot；5% ferric trichloride ethanol solution of the hydrochloric acid acidification is prepared as follows：Ferric trichloride 5g is taken, ethyl alcohol is added to dissolve
And be diluted to 100ml, add hydrochloric acid 1ml to get；
It checks：By being checked under bagged instant preparation item.
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| CN102626168A (en) * | 2012-04-11 | 2012-08-08 | 徐州绿之野生物食品有限公司 | Ginkgo leaf extraction process and method for processing ginkgo broadleaf holly leaf tea |
| CN103907732B (en) * | 2014-04-18 | 2016-02-10 | 广西壮族自治区中医药研究院 | The preparation method of Ilex Latifolia Thunb black tea |
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