CN105016481A - Aquaculture composite plant fermentation broth and preparation method thereof - Google Patents
Aquaculture composite plant fermentation broth and preparation method thereof Download PDFInfo
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Abstract
The invention relates to the technical field of aquaculture, and specifically relates to an aquaculture composite plant fermentation broth and a preparation method thereof. The fermentation broth adopts mulberry leaf, chrysanthemum stem and leaf, honeysuckle leaf and ginkgo leaf as main raw materials, and adopts molasses and crystal salt as auxiliary materials. After a period of time of pickling, the pickle liquid is adopted as a fermentation mother liquor; a culture solution prepared with molasses, crystal salt and sterile water is added; and secondary fermentation is carried out under a certain temperature for a certain period of time, such that the fermentation broth is obtained. The aquaculture composite plant fermentation broth is prepared from the raw materials of pure natural materials. The production cost is low, the raw material source is wide, and no other chemical additive is used. The fermentation broth is simple to use, and has a good effect. The fermentation broth causes no secondary pollution, and provides a bio-enhancer effect. With the fermentation broth, large amounts of ammonia nitrogen, phosphorus salt, sulfur, microbes, pathogens and the like can be removed, and a significant effect is provided for improving water quality and preventing water pollution.
Description
Technical field
The invention belongs to technical field of aquaculture, be specifically related to a kind of aquaculture composite plant fermented liquid and preparation method thereof.
Background technology
Since eighties of last century seventies, world aquaculture increase of production is rapid, and the proportion in water industry also improves day by day, the consequent, and the pollution of aquaculture water is day by day serious, and the disease problem caused thus is also more and more serious.Some breeding production person ignores problem such as process breeding wastewater, refuse etc., not only cause the deterioration of self aquaculture water ecotope, also detrimentally affect is caused to natural ecosystems, as aging in part freshwater, silting causes water ecology to worsen, directly cause that feed coefficient is high, sickness rate is high, surviving rate is low, yield poorly, directly results in culture benefit low.
Also there is now the method that a lot of process aquaculture purifies water.It generally can be summarized as Physical, chemical method and biological process etc.According to the physico-chemical property of pollutent in water body and water body, adopt mechanical means to purify water, as filtrations, precipitation etc., these Method And Principles simply, but operate and bother very much.Also some are had to utilize pollutent in chemical process process water or suspension micelle, as gel, neutralization, complexing and chemical disinfection.Although these method effects are pretty good, cost is high, and introduces other chemical substance, can cause adverse influence to the growth of fishery products.Some method utilizes microorganism and waterplant to consume the meta-bolites of residual bait and aquatic animal in water body, to reach the effect of purifying water body, as photosynthetic bacterium, waterplant etc., but this single culture, specific aim is too strong, bad adaptability, the narrow deficiency of application surface.
Summary of the invention
In order to solve the problem such as breeding wastewater, waste pollution produced in aquaculture, the object of the present invention is to provide a kind of aquaculture composite plant fermented liquid, and apply it in aquaculture, improve water quality to reach, improve surviving rate, improve output, reduce feed coefficient, improve the aspects such as aquaculture benefit effect.
Another object of the present invention is the preparation method providing this aquaculture composite plant fermented liquid.
The technical solution adopted for the present invention to solve the technical problems is: aquaculture composite plant fermented liquid of the present invention is main raw material by mulberries leaf, chrysanthemum cauline leaf, leaf of Flos Lonicerae and Ginkgo Leaf, molasses and crystal salt are auxiliary material, through pickling after a while, and be fermentation mother liquor with pickling liquid, add the nutrient solution of molasses, crystal salt, sterilized water preparation again, Secondary Fermentation is carried out, a kind of natural composite plant fermented liquid obtained in certain temperature with in the time.
The preparation method of aquaculture composite plant fermented liquid of the present invention, comprises the following steps:
(1) plant mixture, molasses and crystal salt are placed in curing container compacting sealed pickling according to the ratio that mass ratio is 5 ~ 15:1 ~ 7:0.1 ~ 1.5, pickling temperature is 10 ~ 40 DEG C, and salting period is 25 ~ 35 days, obtains pickling liquid;
(2) by molasses, crystal salt, sterilized water according to mass ratio be the proportions nutrient solution of 20 ~ 55:0.1 ~ 2:1000 ~ 2500;
(3) by the pickling liquid of step (1) gained and the mixing of step (2) gained nutrient solution; utilize emulsification shearing pump circulation 1-3 hour; by high pressure homogenizer, micronize is carried out 1 ~ 3 hour to it more afterwards; homogenization pressure is 5 ~ 40MPa; temperature is normal temperature; then Secondary Fermentation 2 ~ 10 days under 20 ~ 40 DEG C of leavening temperatures, obtains composite plant fermented liquid.
Plant mixture in described step (1) is made up of mulberries leaf, chrysanthemum cauline leaf, leaf of Flos Lonicerae, Ginkgo Leaf, and wherein the weight proportion of mulberries leaf, chrysanthemum cauline leaf, leaf of Flos Lonicerae, Ginkgo Leaf is 30 ~ 70:20 ~ 50:10 ~ 30:10 ~ 30.Because mulberries leaf, chrysanthemum cauline leaf, leaf of Flos Lonicerae, Ginkgo Leaf self are just with a lot of microorganism, be more conducive to the increase of prior fermentation microbial growth reproduction speed, so the present invention adopts mulberries leaf, chrysanthemum cauline leaf, leaf of Flos Lonicerae, Ginkgo Leaf to ferment.
The operation steps of pickling in described step (1) is: be laid in bottom aseptic curing container by mulberries leaf, chrysanthemum cauline leaf, leaf of Flos Lonicerae, Ginkgo Leaf after 30 ~ 70:20 ~ 50:10 ~ 30:10 ~ 30 mix, in proportion crystal salt is sprinkling upon equably above-mentioned mixing natural phant surface, then in proportion molasses is watered in the superficial compaction of above-mentioned mixing natural phant; Afterwards by said sequence repetitive operation until be paved with curing container.
More specifically, the operation steps of pickling in described step (1) is: be laid in bottom aseptic curing container by mulberries leaf, chrysanthemum cauline leaf, leaf of Flos Lonicerae, Ginkgo Leaf after 30 ~ 70:20 ~ 50:10 ~ 30:10 ~ 30 mix, when sending into Jar used for making 1/10 position, in proportion crystal salt is sprinkling upon equably above-mentioned mixing natural phant surface, then in proportion molasses is watered in the superficial compaction of above-mentioned mixing natural phant; And then cover the mixing raw material of upper 1/10 ... afterwards by said sequence repetitive operation until be paved with curing container.
Why adopt compacting sealed pickling, main purpose is under the prerequisite of compacting sealing, can prevent air to enter in Jar used for making thus produce other miscellaneous bacterias, simultaneously for the fermentation of probiotic bacterium provides condition, the probiotic bacterium produced due to the present invention belongs to anerobe, and the situation of compacting sealing can provide good environment for it.Molasses provide nutrition and energy for fermentable, and crystal salt provides the living environment of a less salt for microorganism.
Preferably, in described step (3), the volume ratio of pickling liquid and nutrient solution is 0.1 ~ 2:10 ~ 30, nutrient solution provides nutrition for the growth and breeding of the plants probiotics in pickling liquid, through emulsification shearing pump circulation 1 ~ 3 hour after mixing, in order to increase the stability of nutrient solution, improve the growth and breeding speed of probiotic bacterium in treatment agent.
The aquaculture composite plant fermented liquid obtained by aforesaid method, its pH is 2 ~ 4, and viable count is 10
5~ 10
10cFU/mL.
After adopting the plant stem-leaf selected by the present invention to carry out curing and fermentation, a large amount of plants probiotics can be produced as plant lactobacillus group, yeast flora, photosynthetic bacterium etc., these plants probiotics have promoting digestion and absorption, improve anti-adversity ability, obviously improve the effect of fish, shrimp, crab, the frog, shellfish healthy state; Frequent use composite plant fermented liquid can effectively improve water quality, promote residual bait and other be floating with the objectionable impurities such as decomposition, degradation of ammonia nitrogen, nitrite, hydrogen sulfide of machine thing, increase oxygen in water, promote useful planktonic growth in water body; Composite plant fermented liquid can also adjust breeding ecological environment, improves dissolved oxygen in water, promotes the normal microflora in cultivating system and beneficial algae activated growth, keeps the eubiosis of aquaculture water; Pathogenic micro-organism and bad algae can be reduced; Obvious immunizing power and the disease resistance strengthening cultivation object, improves surviving rate.Fermentation and curing process are entirely biological treatment process simultaneously, and can not produce other pollutent, also unlike method of chemical treatment, need a large amount of chemical reagent, the impact for environment is minimum, can promotion and application on a large scale.
The aquaculture composite plant fermented liquid that the present invention obtains, can be directly used in freshwater fish culturing, such as soft-shelled turtle, mandarin fish, snakeheaded fish, also may be used in culturing marine products, thus reduce the growth of pathogenic micro-organism and bad algae, stablize and improve water water quality, growth promoting effects, improves rate of body weight gain.In breeding process, its input amount is that 10kg/hm2 ~ 150kg/hm2 can obtain splendid effect.Why controlling above-mentioned consumption is to ensure that the quantity of probiotic bacterium reaches the minimum requirements of process, and in the too small then treatment agent of addition, probiotic activity is too small, does not reach the effect that process improves water quality; Addition is excessive, wastes and may make body eutrophication.Play a cleaning action in order to continual to water quality, promote fingerling growth, the input cycle of described aquaculture composite plant fermented liquid is 5-20 days.
Aquaculture composite plant fermented liquid of the present invention, natural material is adopted to be raw material preparation, production cost is low, and raw material sources are extensive, do not adopt other chemical additive, use simple, effective, non-secondary pollution, can play the effect of bio-enhancer, remove a large amount of ammonia nitrogen, microcosmic salt, sulphur, microorganism, pathogenic agent etc., to improving water quality, prevented water pollution unusual effect.
Embodiment
Be below specific embodiments of the invention, technical scheme of the present invention is described further, but protection scope of the present invention is not limited to these embodiments.Every do not deviate from the present invention's design change or equivalent substituting include within protection scope of the present invention.
Specific embodiment of the invention is for breeding soft-shell turtle.
Embodiment 1
1, a preparation method for aquaculture composite plant fermented liquid, its concrete steps are as follows:
(1) the natural phant cauline leafs such as mulberries leaf, chrysanthemum cauline leaf, leaf of Flos Lonicerae, Ginkgo Leaf are laid in bottom aseptic curing container after 50:30:20:20 mixes, crystal salt is sprinkling upon equably above-mentioned mixing natural phant surface, then watered by molasses in the superficial compaction of above-mentioned mixing natural phant, the weight ratio of described mixing natural phant, molasses, crystal salt is 5:1:0.1; Afterwards by said sequence repetitive operation until be paved with curing container, pickling temperature is 10 DEG C, and salting period is 35 days, obtains pickling liquid;
(2) by molasses, crystal salt, sterilized water according to mass ratio be the proportions nutrient solution of 20:0.1:1000;
(3) by the pickling liquid of step (1) gained and the mixing of step (2) gained nutrient solution, emulsification shearing pump is utilized to circulate 1 hour, carry out micronize 3 hours by high pressure homogenizer to it more afterwards, homogenization pressure is 5MPa, and temperature is normal temperature; Then Secondary Fermentation 10 days under 40 DEG C of leavening temperatures, obtains composite plant fermented liquid; Wherein, the volume ratio of pickling liquid and nutrient solution is 2:10.
Gained aquaculture composite plant fermented liquid pH is 2; Viable count is 10
5cFU/mL.
2, the breeding process of soft-shelled turtle, its detailed process is:
(1) preparation of ponds and water conditioning
The pond chosen occupies 500m2, keeps mud to be 5 ~ 10cm at thickness, then uses unslaked lime 1000kg/hm
2dry disinfection, harrows 1 time on the 2nd day again, in the hope of sterilizing thoroughly, and unfused lime block is picked pond.The 3rd day after sterilization starts water filling.Before putting seedling, water body uses the fertilizer 3000kg/hm of fermentation
2, make water body become yellow-green colour.
(2) seed is put in a suitable place to breed
In pond, put into 1000 soft-shelled turtles, soft-shelled turtle specification at 500 ~ 750g/ only, amounts to 575.6kg.And body surface is glossy, stands up and take action active, without wound, residual, worm, disease.Before seed is put in a suitable place to breed, all cleaned through the salt solution of 3%, to go out the parasite of soft-shelled turtle body surface and germ.
(3) forage feed
According to day throw the amount of raising be 8% of soft-shelled turtle gross weight carry out every day feed throw in, every day feeds twice, and the time is respectively 6:00 and 16:00.Small fish and shrimp as food also just can will be thrown something and fed after rinsing and sterilization.
(4) composite plant fermented liquid is dropped into
The controlled trial that the present embodiment is thrown in as composite plant fermented liquid, therefore do not throw in any composite plant fermented liquid.
(5) daily observation
Check the integrated environment changing conditions in soft-shelled turtle pond, food situation etc. every day, and note observing pond change of water quality, get soft-shelled turtle and put the day before yesterday in a suitable place to breed, put the pond water sample of second day, the 5th day in a suitable place to breed, survey dissolved oxygen, pH value, ammonia nitrogen, the nitrite nitrogen index of water quality.
Embodiment 2
1, a preparation method for aquaculture composite plant fermented liquid, its concrete steps are as follows:
(1) the natural phant cauline leafs such as mulberries leaf, chrysanthemum cauline leaf, leaf of Flos Lonicerae, Ginkgo Leaf are laid in bottom aseptic curing container after 70:50:15:10 mixes, crystal salt is sprinkling upon equably above-mentioned mixing natural phant surface, then watered by molasses in the superficial compaction of above-mentioned mixing natural phant, the weight ratio of described mixing natural phant, molasses, crystal salt is 15:7:1.5; Afterwards by said sequence repetitive operation until be paved with curing container, pickling temperature is 40 DEG C, and salting period is 25 days, obtains pickling liquid;
(2) by molasses, crystal salt, sterilized water according to mass ratio be the proportions nutrient solution of 55:2:2500;
(3) by the pickling liquid of step (1) gained and the mixing of step (2) gained nutrient solution, emulsification shearing pump is utilized to circulate 3 hours, carry out micronize 1 hour by high pressure homogenizer to it more afterwards, homogenization pressure is 40MPa, and temperature is normal temperature; Then Secondary Fermentation 2 days under 20 DEG C of leavening temperatures, obtains composite plant fermented liquid; Wherein, the volume ratio of pickling liquid and nutrient solution is 0.1:30.
Gained aquaculture composite plant fermented liquid pH is 4; Viable count is 10
6cFU/mL.
2, the breeding process of soft-shelled turtle, its detailed process is:
(1) preparation of ponds and water conditioning
The pond chosen is 500m2, keeps mud to be 5 ~ 10cm at thickness, then uses unslaked lime 800kg/hm
2dry disinfection, harrows 1 time on the 2nd day again, in the hope of sterilizing thoroughly, and unfused lime block is picked pond.The 3rd day after sterilization starts water filling.Before putting seedling, water body uses the inorganic composite fertilizer 100kg/hm of fermentation
2, make water body become yellow-green colour.
(2) seed is put in a suitable place to breed
In pond, put into 1000 soft-shelled turtles, soft-shelled turtle specification at 500 ~ 750g/ only, amounts to 587.2kg.And body surface is glossy, stands up and take action active, without wound, residual, worm, disease.Before seed is put in a suitable place to breed, all cleaned through the salt solution of 3%, to go out the parasite of soft-shelled turtle body surface and germ.
(3) forage feed
According to day throw the amount of raising be 8% of soft-shelled turtle gross weight carry out every day feed throw in, every day feeds twice, and the time is respectively 6:00 and 16:00.Small fish and shrimp as food also just can will be thrown something and fed after rinsing and sterilization.
(4) composite plant fermented liquid is dropped into
This is thrown according to 99.85kg/hm
2, therefore in pond, add the plant fermented liquid of 4.99kg, within every 15 days, throw in once.
(5) daily observation
Check the integrated environment changing conditions in soft-shelled turtle pond, food situation etc. every day, and note observing pond change of water quality, get soft-shelled turtle and put the day before yesterday in a suitable place to breed, put the pond water sample of second day, the 5th day in a suitable place to breed, survey dissolved oxygen, pH value, ammonia nitrogen, the nitrite nitrogen index of water quality.
Embodiment 3
1, a preparation method for aquaculture composite plant fermented liquid, its concrete steps are as follows:
(1) the natural phant cauline leafs such as mulberries leaf, chrysanthemum cauline leaf, leaf of Flos Lonicerae, Ginkgo Leaf are laid in bottom aseptic curing container after 30:20:10:30 mixes, crystal salt is sprinkling upon equably above-mentioned mixing natural phant surface, then watered by molasses in the superficial compaction of above-mentioned mixing natural phant, the weight ratio of described mixing natural phant, molasses, crystal salt is 10:4:0.8; Afterwards by said sequence repetitive operation until be paved with curing container, pickling temperature is 25 DEG C, and salting period is 30 days, obtains pickling liquid;
(2) by molasses, crystal salt, sterilized water according to mass ratio be the proportions nutrient solution of 35:1.0:2500;
(3) by the pickling liquid of step (1) gained and the mixing of step (2) gained nutrient solution, emulsification shearing pump is utilized to circulate 2 hours, carry out micronize 1.5 hours by high pressure homogenizer to it more afterwards, homogenization pressure is 22MPa, and temperature is normal temperature; Then Secondary Fermentation 8 days under 35 DEG C of leavening temperatures, obtains composite plant fermented liquid; Wherein, the volume ratio of pickling liquid and nutrient solution is 1.1:20.
Gained aquaculture composite plant fermented liquid pH is 3; Viable count is 10
10cFU/mL.
2, the breeding process of soft-shelled turtle, its detailed process is:
(1) preparation of ponds and water conditioning
The pond chosen is 500m2, keeps mud to be 5 ~ 10cm at thickness, then uses unslaked lime 1300kg/hm
2dry disinfection, harrows 1 time on the 2nd day again, in the hope of sterilizing thoroughly, and unfused lime block is picked pond.The 3rd day after sterilization starts water filling.Before putting seedling, water body uses the fertilizer 4000kg/hm of fermentation
2, make water body become yellow-green colour.
(2) seed is put in a suitable place to breed
In pond, put into 1000 soft-shelled turtles, soft-shelled turtle specification at 500 ~ 750g/ only, amounts to 548.2kg.And body surface is glossy, stands up and take action active, without wound, residual, worm, disease.Before seed is put in a suitable place to breed, all cleaned through the salt solution of 4%, to go out the parasite of soft-shelled turtle body surface and germ.
(3) forage feed
According to day throw the amount of raising be 8% of soft-shelled turtle gross weight carry out every day feed throw in, every day feeds twice, and the time is respectively 6:00 and 16:00.Small fish and shrimp as food also just can will be thrown something and fed after rinsing and sterilization.
(4) composite plant fermented liquid is dropped into
This is thrown according to 124.25kg/hm
2, therefore in pond, add the plant fermented liquid of 6.22kg, within every 15 days, throw in once.
(5) daily observation
Check the integrated environment changing conditions in soft-shelled turtle pond, food situation etc. every day, and note observing pond change of water quality, get soft-shelled turtle and put the day before yesterday in a suitable place to breed, put the pond water sample of second day, the 5th day in a suitable place to breed, survey dissolved oxygen, pH value, ammonia nitrogen, the nitrite nitrogen index of water quality.
Embodiment 4
1, an aquaculture composite plant fermented liquid preparation method, its concrete steps are as follows:
(1) the natural phant cauline leafs such as mulberries leaf, chrysanthemum cauline leaf, leaf of Flos Lonicerae, Ginkgo Leaf are laid in bottom aseptic curing container after 60:40:30:15 mixes, crystal salt is sprinkling upon equably above-mentioned mixing natural phant surface, then watered by molasses in the superficial compaction of above-mentioned mixing natural phant, the weight ratio of described mixing natural phant, molasses, crystal salt is 12:5.5:1.1; Afterwards by said sequence repetitive operation until be paved with curing container, pickling temperature is 30 DEG C, and salting period is 32 days, obtains pickling liquid;
(2) by molasses, crystal salt, sterilized water according to mass ratio be the proportions nutrient solution of 45:1.5:2000;
(3) by the pickling liquid of step (1) gained and the mixing of step (2) gained nutrient solution, emulsification shearing pump is utilized to circulate 1.5 hours, carry out micronize 2 hours by high pressure homogenizer to it more afterwards, homogenization pressure is 30MPa, and temperature is normal temperature; Then Secondary Fermentation 6 days under 30 DEG C of leavening temperatures, obtains composite plant fermented liquid; Wherein, the volume ratio of pickling liquid and nutrient solution is 1.5:25.
Gained aquaculture composite plant fermented liquid pH is 4; Viable count is 10
8cFU/mL.
2, the breeding process of soft-shelled turtle, its detailed process is:
(1) preparation of ponds and water conditioning
The pond chosen is 500m2, keeps mud to be 5 ~ 10cm at thickness, then uses unslaked lime 1500kg/hm
2dry disinfection, harrows 1 time on the 2nd day again, in the hope of sterilizing thoroughly, and unfused lime block is picked pond.The 3rd day after sterilization starts water filling.Before putting seedling, water body uses the fertilizer 3700kg/hm of fermentation
2, make water body become yellow-green colour.
(2) seed is put in a suitable place to breed
In pond, put into 1000 soft-shelled turtles, soft-shelled turtle specification at 500 ~ 750g/ only, amounts to 602.3kg.And body surface is glossy, stands up and take action active, without wound, residual, worm, disease.Before seed is put in a suitable place to breed, all cleaned through the salt solution of 5%, to go out the parasite of soft-shelled turtle body surface and germ.
(3) forage feed
According to day throw the amount of raising be 10% of soft-shelled turtle gross weight carry out every day feed throw in, every day feeds twice, and the time is respectively 6:00 and 16:00.Small fish and shrimp as food also just can will be thrown something and fed after rinsing and sterilization.
(4) composite plant fermented liquid is dropped into
This throws in plant fermented liquid according to 60.23kg/hm
2, therefore in pond, add the plant fermented liquid of 3.01kg, within every 15 days, throw in once.
(5) daily observation
Check the integrated environment changing conditions in soft-shelled turtle pond, food situation etc. every day, and note observing pond change of water quality, get soft-shelled turtle and put the day before yesterday in a suitable place to breed, put the pond water sample of second day, the 5th day in a suitable place to breed, survey dissolved oxygen, pH value, ammonia nitrogen, the nitrite nitrogen index of water quality.
Embodiment 5
The present embodiment to the aquaculture composite plant fermented liquid described in above-described embodiment 1-4 to the checking improving change of water quality.In Example 1,2,3,4, soft-shelled turtle puts into the day before yesterday, puts into the water of latter second day, the 5th day, surveys its dissolved oxygen, pH value, ammonia nitrogen, nitrite nitrogen index.
Detection method:
Dissolved oxygen: GB/T 7489-1987, the dissolved oxygen in water quality measures;
PH value: GB 6920-86, the PH of water quality measures;
Ammonia nitrogen: HJ 537-2009, the mensuration of ammonia nitrogen in water quality;
Nitrite nitrogen: GB/T 7493-1987, the mensuration of water quality Nitrite Nitrogen;
Detected result is as following table:
As can be seen from the above table, pond after employing composite plant fermented liquid, the content of the dissolved oxygen in water quality, ammonia nitrogen, nitrite declines all to some extent, and pH value also declines, therefore composite plant fermented liquid of the present invention has unusual effect to improving water quality in aquaculture.
Embodiment 6
The present embodiment is mainly to a checking of the soft-shelled turtle output that several embodiment before cultivates out.
The result is as follows:
| Embodiment | Feeding period/d | Surviving rate/% | Adult fish output/kg | Average size/kg |
| 1 | 192 | 88 | 1354 | 1.54 |
| 2 | 192 | 92 | 1530 | 1.66 |
| 3 | 192 | 93 | 1592 | 1.71 |
| 4 | 192 | 90 | 1429 | 1.59 |
As can be seen from the above table, the pond of having dropped into composite plant fermented liquid is than the pond of not throwing fermented liquid, and in equal feeding time, surviving rate wants high, and the weight of output soft-shelled turtle is also heavy than not throwing will looking of plant fermented liquid.Learn thus, the raising of composite plant fermented liquid to the output of soft-shelled turtle also has unusual effect.
Claims (6)
1. an aquaculture composite plant fermented liquid, is characterized in that, its pH is 2 ~ 4, and viable count is 10
5~ 10
10cFU/mL.
2. the preparation method of aquaculture composite plant fermented liquid as claimed in claim 1, is characterized in that, comprise the following steps:
(1) plant mixture, molasses and crystal salt are placed in curing container compacting sealed pickling according to the ratio that mass ratio is 5 ~ 15:1 ~ 7:0.1 ~ 1.5, pickling temperature is 10 ~ 40 DEG C, and salting period is 25 ~ 35 days, obtains pickling liquid;
(2) by molasses, crystal salt, sterilized water according to mass ratio be the proportions nutrient solution of 20 ~ 55:0.1 ~ 2:1000 ~ 2500;
(3) by the pickling liquid of step (1) gained and the mixing of step (2) gained nutrient solution; utilize emulsification shearing pump circulation 1-3 hour; by high pressure homogenizer, micronize is carried out 1 ~ 3 hour to it more afterwards; homogenization pressure is 5 ~ 40MPa; temperature is normal temperature; then Secondary Fermentation 2 ~ 10 days under 20 ~ 40 DEG C of leavening temperatures, obtains composite plant fermented liquid.
3. the preparation method of aquaculture composite plant fermented liquid according to claim 2, it is characterized in that, plant mixture in described step (1) is made up of mulberries leaf, chrysanthemum cauline leaf, leaf of Flos Lonicerae, Ginkgo Leaf, and wherein the weight proportion of mulberries leaf, chrysanthemum cauline leaf, leaf of Flos Lonicerae, Ginkgo Leaf is 30 ~ 70:20 ~ 50:10 ~ 30:10 ~ 30.
4. the preparation method of aquaculture composite plant fermented liquid according to claim 3, it is characterized in that, the operation steps of pickling in described step (1) is: be laid in bottom aseptic curing container by mulberries leaf, chrysanthemum cauline leaf, leaf of Flos Lonicerae, Ginkgo Leaf after 30 ~ 70:20 ~ 50:10 ~ 30:10 ~ 30 mix, in proportion crystal salt is sprinkling upon equably above-mentioned mixing natural phant surface, then in proportion molasses is watered in the superficial compaction of above-mentioned mixing natural phant; Afterwards by said sequence repetitive operation until be paved with curing container.
5. the preparation method of the aquaculture composite plant fermented liquid according to claim 3 or 4, it is characterized in that, the operation steps of pickling in described step (1) is specially: be laid in bottom aseptic curing container by mulberries leaf, chrysanthemum cauline leaf, leaf of Flos Lonicerae, Ginkgo Leaf after 30 ~ 70:20 ~ 50:10 ~ 30:10 ~ 30 mix, when sending into Jar used for making 1/10 position, in proportion crystal salt is sprinkling upon equably above-mentioned mixing natural phant surface, then in proportion molasses is watered in the superficial compaction of above-mentioned mixing natural phant; And then cover the mixing raw material of upper 1/10 ... afterwards by said sequence repetitive operation until be paved with curing container.
6. the preparation method of aquaculture composite plant fermented liquid according to claim 2, is characterized in that, in described step (3), the volume ratio of pickling liquid and nutrient solution is 0.1 ~ 2:10 ~ 30.
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| CN106946363A (en) * | 2017-04-28 | 2017-07-14 | 董金伟 | A kind of method at purifying contaminated water source |
| CN113068717A (en) * | 2021-03-31 | 2021-07-06 | 北京局气网络技术有限公司 | Plant fermentation extract for regulating seawater quality and preparation method thereof |
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| CN101591631A (en) * | 2009-06-08 | 2009-12-02 | 江西省天意生物技术开发有限公司 | EM original solution and production method thereof |
| CN103409337A (en) * | 2013-07-01 | 2013-11-27 | 青岛浩澳环保科技有限公司 | Extracting raw material and extracting method of photosynthetic bacteria |
| CN104082345A (en) * | 2014-07-24 | 2014-10-08 | 中国水产科学研究院珠江水产研究所 | Special EM (Effective Microorganisms) bacteria powder for aquaculture and preparation method and application of special EM (Effective Microorganisms) bacteria powder |
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| CN106946363A (en) * | 2017-04-28 | 2017-07-14 | 董金伟 | A kind of method at purifying contaminated water source |
| CN113068717A (en) * | 2021-03-31 | 2021-07-06 | 北京局气网络技术有限公司 | Plant fermentation extract for regulating seawater quality and preparation method thereof |
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