CN105004698A - Novel surface plasmon resonance biosensor - Google Patents
Novel surface plasmon resonance biosensor Download PDFInfo
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- CN105004698A CN105004698A CN201510313359.9A CN201510313359A CN105004698A CN 105004698 A CN105004698 A CN 105004698A CN 201510313359 A CN201510313359 A CN 201510313359A CN 105004698 A CN105004698 A CN 105004698A
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- surface plasmon
- plasmon resonance
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- graphene
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- 238000002198 surface plasmon resonance spectroscopy Methods 0.000 title claims abstract description 22
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 38
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 29
- 239000000377 silicon dioxide Substances 0.000 claims abstract description 19
- 229910021389 graphene Inorganic materials 0.000 claims abstract description 18
- 239000000463 material Substances 0.000 claims abstract description 9
- 239000004793 Polystyrene Substances 0.000 claims abstract description 8
- 229920002223 polystyrene Polymers 0.000 claims abstract description 8
- 239000012528 membrane Substances 0.000 claims description 15
- 239000002077 nanosphere Substances 0.000 claims description 11
- 239000004411 aluminium Substances 0.000 claims description 3
- 229910052782 aluminium Inorganic materials 0.000 claims description 3
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 claims description 3
- 239000005308 flint glass Substances 0.000 claims description 3
- XOCUXOWLYLLJLV-UHFFFAOYSA-N [O].[S] Chemical compound [O].[S] XOCUXOWLYLLJLV-UHFFFAOYSA-N 0.000 claims description 2
- 239000011521 glass Substances 0.000 claims description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 2
- 239000010931 gold Substances 0.000 claims description 2
- 229910052737 gold Inorganic materials 0.000 claims description 2
- 239000010703 silicon Substances 0.000 claims description 2
- 229910052710 silicon Inorganic materials 0.000 claims description 2
- 229910052751 metal Inorganic materials 0.000 abstract description 7
- 239000002184 metal Substances 0.000 abstract description 7
- 239000010409 thin film Substances 0.000 abstract description 5
- 238000001514 detection method Methods 0.000 abstract description 4
- 230000035945 sensitivity Effects 0.000 abstract description 4
- 235000012239 silicon dioxide Nutrition 0.000 abstract description 4
- 230000000694 effects Effects 0.000 abstract description 3
- 239000000126 substance Substances 0.000 abstract description 3
- 238000007254 oxidation reaction Methods 0.000 abstract description 2
- 239000002245 particle Substances 0.000 abstract 4
- 239000010408 film Substances 0.000 description 9
- 238000005516 engineering process Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 3
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 2
- 238000005229 chemical vapour deposition Methods 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000007797 corrosion Effects 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 230000005672 electromagnetic field Effects 0.000 description 1
- 238000005538 encapsulation Methods 0.000 description 1
- 239000003574 free electron Substances 0.000 description 1
- 239000007970 homogeneous dispersion Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 238000001755 magnetron sputter deposition Methods 0.000 description 1
- 239000011807 nanoball Substances 0.000 description 1
- 239000002086 nanomaterial Substances 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 230000010287 polarization Effects 0.000 description 1
- 238000000985 reflectance spectrum Methods 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
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- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention discloses a novel surface plasmon resonance biosensor and belongs to the field of nano-science. The novel surface plasmon resonance biosensor is composed of a prism (1), a nanometer particle array (2), a silicon dioxide thin film (3), graphene (4) and a sample pool (5). The nanometer particle array (2) is located on the lower surface of the prism (1) and covered with the silicon dioxide thin film (3), and the silicon dioxide thin film (3) is covered with the graphene (4). The novel surface plasmon resonance biosensor is characterized in that the prism (1) is made of high-refraction-rate material, and the nanometer particle array (2) is formed by polystyrene nanometer balls (6) covered with a metal thin film (7). On the basis of an SPR principle, SPPs are excited on the lower surface of metal, and the small change of the refraction rate of a sample can affect a resonance curve and change the resonance angle; otherwise, according to the resonance angle obtained through detection, the refraction rate of the sample can be obtained through deduction, and then the concentration of substances to be detected can be accurately obtained. The nanometer particle array is utilized for exciting the sensitive feature of the SPPs, the geometric structure features of the graphene are utilized for adsorbing molecules to be detected better, meanwhile, the effect of protecting the metal from oxidization is achieved, and the purpose of improving the sensitivity and accuracy of the biosensor is achieved.
Description
Technical field
The present invention relates to a kind of novel surface plasmon resonance biology sensor, belong to nanometer technology, biological technology field.
Background technology
In recent years, considerable progress is achieved to the research of metal surface phasmon (SPP).SPP refers to the collective oscillation by external electromagnetic field (as light wave) inducing metal micro-nano structure surface free electron, it has an outstanding feature can realize surface plasmon resonance (Surface Plasmon Resonance exactly, SPR), during resonance, internal field can increase thousands of times.Therefore the action effect of electric field and material can be improved greatly, very extensive in the application of bio-sensing field, become the strong technological means of people's amalyzing substances concentration.The present invention proposes a kind of novel SPR sensing technology, adopt outside nanosphere and cover metallic film as sensing unit, and with Graphene encapsulation, have the advantages that sensitivity is high and applied widely.
Summary of the invention
The technical issues that need to address of the present invention are: overcome the deficiencies in the prior art, provide a kind of highly sensitive, have wide range of applications, a kind of novel surface plasmon resonance biology sensor of price economy.
Technical solution of the present invention is:
A kind of novel surface plasmon resonance biology sensor, be made up of prism (1), nano-grain array (2), silica membrane (3), Graphene (4) and sample cell (5), nano-grain array (2) is positioned at the lower surface of prism (1), silica membrane (3) covers on nano-grain array (2), Graphene (4) covers on silica membrane (3), prism (1) is high-index material, and nano-grain array (2) forms by covering metallic film (7) outside polystyrene nanospheres (6).
Described prism (1) is greater than the material of silica membrane (3) for refractive index, and is low-loss relative to incident light, is preferably dense flint glass or seleno oxygen sulphur glass or silicon.
Described polystyrene nanospheres (6) diameter is 50nm ~ 80nm, individual layer next-door neighbour arrangement.
Described metallic film (7) material is gold or silver-colored or aluminium, and thickness is 10nm ~ 80nm.
Described silica membrane (3) thickness is between 10nm and 0.25 times incident wavelength.
Described Graphene (4) number of plies is 1 ~ 8 layer.
The present invention compared with prior art tool has the following advantages:
1. the sensitivity of superelevation.The surface plasmon resonance biosensor of the present invention's design improves 1 magnitude nearly with traditional biology sensor phase specific sensitivity.
2. test substance sensing range kind is wide.This biology sensor overcomes the limited shortcoming of testing sample states of matter, can carry out liquid state detection and can carry out gaseous state detection, applied range again.
3. long service life.Graphene has very strong oxidation resistance, can the not oxidized and corrosion of guard metal layer, increases serviceable life of sensor.
Operative wavelength is wide.The prism material that the present invention selects has wider operative wavelength, can detect at different-waveband to sample.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention;
Fig. 2 surface plasmon resonance peak;
The resonance peak that Fig. 3 tests sample moves.
Embodiment
Below in conjunction with drawings and Examples, the invention will be further described.
As shown in Figure 1, the present invention is made up of prism (1), nano-grain array (2), silica membrane (3), Graphene (4) and sample cell (5), nano-grain array (2) is positioned at the lower surface of prism (1), silica membrane (3) covers on nano-grain array (2), and Graphene (4) covers on silica membrane (3).When prism (1) is dense flint glass, metallic film (7) is silver, and thickness h is 10nm, and silica membrane (3) thickness is 25nm, when Graphene (4) thickness is 0.34nm, is the surface plasmon resonance that incident wavelength 700nm is used.
When incident by the 700nm LASER Light Source of TM polarization, after prism (1) refraction, arrive nano-grain array (2).After incident angle is greater than the cirtical angle of total reflection, produce evanescent waves in the interface of prism (1) and nano-grain array (2), this evanescent waves is by excitating surface plasmon resonance pattern.When to be covered in metallic film (7) thickness h on polystyrene nanospheres (6) be 10nm ~ 80nm, can significantly strengthen this resonance mode, its effect is similar to nano ball shell.When resonance occurs, internal field increases, and incident light is absorbed, and energy of reflection light is sharply declined, and reflectance spectrum occurs surface plasmon resonance peak, as shown in Figure 2.This resonance peak is very responsive to the change of sample refractive index, and when the sample refraction index changing adsorbed by Graphene (4), resonant positions will change, and realize the detection to sample, as shown in Figure 3.Graphene (4) specific inductive capacity has larger imaginary part, at a visible light wave range order of magnitude larger than Ag.When this can cause surface plasmon resonance, loss increases, and causes resonance peak to broaden, the accuracy of sensor is reduced.Between metallic film (7) and Graphene (4), add the effective dielectric constant that silica membrane (3) regulates film system, thickness is between 10nm and 0.25 times incident wavelength.
Concrete making step of the present invention is as follows:
A. polystyrene nanospheres homogeneous dispersion is coated in prism lower surface, forms the individual layer nanosphere array of next-door neighbour; B. adopt magnetron sputtering metal-coated films on the nanosphere array of prism lower surface under vacuum environment, thickness is 10nm ~ 80nm; C. adopt liquid-phase deposition technique method (LPD) silicon dioxide thin film growth on argent or aluminium film, thickness is 10nm ~ 0.25 times incident wavelength; D. adopt chemical vapour deposition technique (CVD) growing graphene, thickness is 0.34nm ~ 2.72nm; E. clean, dry, complete making.
Claims (6)
1. a novel surface plasmon resonance biology sensor, be made up of prism (1), nano-grain array (2), silica membrane (3), Graphene (4) and sample cell (5), nano-grain array (2) is positioned at the lower surface of prism (1), silica membrane (3) covers on nano-grain array (2), Graphene (4) covers on silica membrane (3), it is characterized in that: prism (1) is high-index material, nano-grain array (2) forms by covering metallic film (7) outside polystyrene nanospheres (6).
2. novel surface plasmon resonance biology sensor according to claim 1, it is characterized in that: described prism (1) is greater than the material of silica membrane (3) for refractive index, and be low-loss relative to incident light, be preferably dense flint glass or seleno oxygen sulphur glass or silicon.
3. novel surface plasmon resonance biology sensor according to claim 1, is characterized in that: described polystyrene nanospheres (6) diameter is 50nm ~ 80nm, individual layer next-door neighbour arrangement.
4. novel surface plasmon resonance biology sensor according to claim 1, is characterized in that: described metallic film (7) covers on polystyrene nanospheres (6), and distance nanosphere top thickness h is 10nm ~ 80nm, and material is gold or silver-colored or aluminium.
5. novel surface plasmon resonance biology sensor according to claim 1, is characterized in that: described silica membrane (3) thickness is between 10nm and 0.25 times incident wavelength.
6. novel surface plasmon resonance biology sensor according to claim 1, is characterized in that: described Graphene (4) number of plies is 1 ~ 8 layer.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510313359.9A CN105004698B (en) | 2015-06-09 | 2015-06-09 | A kind of Biosensors Based on Surface Plasmon Resonance device |
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| CN201510313359.9A CN105004698B (en) | 2015-06-09 | 2015-06-09 | A kind of Biosensors Based on Surface Plasmon Resonance device |
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| CN105004698B CN105004698B (en) | 2018-01-09 |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105372207A (en) * | 2015-11-25 | 2016-03-02 | 广西师范大学 | Graphene material surface plasmon waveguide trace gas sensing device |
| CN106872405A (en) * | 2017-01-05 | 2017-06-20 | 深圳大学 | A kind of biologic sensor chip based on bilayer graphene |
| CN109870765A (en) * | 2019-03-22 | 2019-06-11 | 中国科学院微电子研究所 | A kind of super resonance of gain auxiliary based on single silicon line |
| CN110231309A (en) * | 2019-04-15 | 2019-09-13 | 厦门大学 | A method of utilizing the medium prism structure and its sensing of graphene |
| CN111239082A (en) * | 2020-01-21 | 2020-06-05 | 东北大学秦皇岛分校 | Surface plasma resonance sensor based on graphene-silver grating |
| CN111272666A (en) * | 2020-02-27 | 2020-06-12 | 电子科技大学 | Bio-protein sensor based on magneto-optical surface plasmon resonance |
| CN111735799A (en) * | 2020-07-02 | 2020-10-02 | 燕山大学 | Guided wave surface plasma resonance sensor |
| CN112304905A (en) * | 2020-10-22 | 2021-02-02 | 济南橘子智能科技有限公司 | High-performance waveguide sensing system and preparation method thereof |
| CN113030026A (en) * | 2021-03-07 | 2021-06-25 | 天津理工大学 | LSPR multi-wavelength narrow-band tunable sensor |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109917512B (en) * | 2019-03-22 | 2020-07-28 | 中国科学院微电子研究所 | Silicon double-wire system with gain assistance |
| CN112033932B (en) * | 2020-09-07 | 2021-09-07 | 科竟达生物科技有限公司 | Local surface plasmon resonance biochip, manufacturing method thereof, biosensing system comprising local surface plasmon resonance biochip and application of biosensing system |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105372207A (en) * | 2015-11-25 | 2016-03-02 | 广西师范大学 | Graphene material surface plasmon waveguide trace gas sensing device |
| CN106872405A (en) * | 2017-01-05 | 2017-06-20 | 深圳大学 | A kind of biologic sensor chip based on bilayer graphene |
| CN106872405B (en) * | 2017-01-05 | 2019-08-13 | 深圳大学 | A kind of biologic sensor chip based on bilayer graphene |
| CN109870765B (en) * | 2019-03-22 | 2020-07-28 | 中国科学院微电子研究所 | Gain-assisted super-resonance based on single silicon wire |
| CN109870765A (en) * | 2019-03-22 | 2019-06-11 | 中国科学院微电子研究所 | A kind of super resonance of gain auxiliary based on single silicon line |
| CN110231309A (en) * | 2019-04-15 | 2019-09-13 | 厦门大学 | A method of utilizing the medium prism structure and its sensing of graphene |
| CN111239082A (en) * | 2020-01-21 | 2020-06-05 | 东北大学秦皇岛分校 | Surface plasma resonance sensor based on graphene-silver grating |
| CN111272666A (en) * | 2020-02-27 | 2020-06-12 | 电子科技大学 | Bio-protein sensor based on magneto-optical surface plasmon resonance |
| CN111272666B (en) * | 2020-02-27 | 2022-01-25 | 电子科技大学 | Bio-protein sensor based on magneto-optical surface plasmon resonance |
| CN111735799A (en) * | 2020-07-02 | 2020-10-02 | 燕山大学 | Guided wave surface plasma resonance sensor |
| CN112304905A (en) * | 2020-10-22 | 2021-02-02 | 济南橘子智能科技有限公司 | High-performance waveguide sensing system and preparation method thereof |
| CN112304905B (en) * | 2020-10-22 | 2022-11-04 | 吉林省兜率龙源实业集团有限公司 | High-performance waveguide sensing system and preparation method thereof |
| CN113030026A (en) * | 2021-03-07 | 2021-06-25 | 天津理工大学 | LSPR multi-wavelength narrow-band tunable sensor |
| CN113030026B (en) * | 2021-03-07 | 2022-11-04 | 天津理工大学 | LSPR multi-wavelength narrow-band tunable sensor |
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Effective date of registration: 20220120 Address after: 264200 No. 21, Xingshan Road, chucun Town, torch high tech Industrial Development Zone, Weihai City, Shandong Province Patentee after: Shandong Weixin Medical Equipment Co.,Ltd. Address before: 241002 Research Office of Anhui Normal University, Huajin South Road, Wuhu City, Anhui Province Patentee before: ANHUI NORMAL University |