CN104997817A - Extraction method of moringa oleifera leaf extractive - Google Patents
Extraction method of moringa oleifera leaf extractive Download PDFInfo
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- CN104997817A CN104997817A CN201510457491.7A CN201510457491A CN104997817A CN 104997817 A CN104997817 A CN 104997817A CN 201510457491 A CN201510457491 A CN 201510457491A CN 104997817 A CN104997817 A CN 104997817A
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- moringa oleifera
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Abstract
The invention discloses an extraction method of moringa oleifera leaf extractive. The extraction method is characterized by comprising the following steps of 1 crushing, wherein moringa oleifera leaves are crushed into 40-100-mesh powder; 2 ultrasonic extracting, wherein the moringa oleifera leaf powder is extracted by adopting an ultrasonic extractor; 4 filtering, wherein frame filtering is performed on an extracting solution, and a material solution processed through frame filtering is filtered through microfiltration to obtain a microfiltration dialysis solution; 5 concentrating, wherein the microfiltration dialysis solution is concentrated through reverse osmosis circulation until the diopter of a reverse osmosis concentrated solution is larger than 10; 6 extracting, wherein the reverse osmosis concentrated solution is extracted by adopting petroleum ether, a water-layer clear solution is collected, and beta-cyclodextrin with 1-3 percent of solid content is added in the supernatant solution and stirred evenly; 7 spraying and drying, wherein spraying and drying are performed to obtain the faint yellow moringa oleifera leaf extractive. According to the extraction method of the moringa oleifera leaf extractive, the unique fragrance of the extractive is maintained while it is guaranteed that the water solubility of a finished product is achieved and the finished product is clear and bright, and meanwhile flavone and polysaccharide in the extractive cannot be lost.
Description
Technical field
The present invention relates to field of plant extraction, particularly a kind of extracting method of moringa oleifera leaf extractive.
Background technology
Moringa is Moringaceae Moringa plant Moringa oleifera, is a kind of tropical plants having unique economic to be worth.Be rich in several mineral materials, vitamin and health care and medicinal chemistry composition in leaf of Moringa, as vegetable and food, extra-nutrition and Dietotherapy health function can be had, also may be used for preventing and treating some diseases.Folk tradition is thought that Moringa has and is brought down a fever, antiinflammatory, calculus, diuresis, blood pressure lowering, pain relieving, heart tonifying, urges the functions such as wish.India and African country are usually used in the various diseases such as treatment diabetes, hypertension, cardiovascular diseases, obesity, dermatosis, ophthalmic, hypoimmunity, vitamin C deficiency, anemia, arthritis, rheumatism, digestive system tumor.The problems such as in prior art, the extraction process ubiquity impurity content of moringa oleifera leaf extractive is high, and effective ingredient loss is serious.
Summary of the invention
For the problems referred to above, the object of the present invention is to provide one guarantee finished product water solublity clarify bright while, also remain the fragrance that this product is special, and flavone polysaccharide in extract can not the extracting method of leaf of Moringa extracting solution of loss.
For achieving the above object, technical scheme proposed by the invention is: a kind of extracting method of moringa oleifera leaf extractive, is characterized in that, comprises the steps:
Step 1 is pulverized: leaf of Moringa is ground into 40-100 object powder;
Step 2 ultrasonic extraction: adopt ultrasonic extractor to carry out supersound extraction in leaf of Moringa powder;
Step 4 is filtered: extracting solution plate-and-frame filtration; Feed liquid after plate-and-frame filtration is filtered by microfiltration, obtains microfiltration dialysis solution, and the film core pore size filter that described microfiltration filters is 3-8 μm;
Step 5 concentrates: microfiltration dialysis solution is concentrated to reverse osmosis concentrated liquid diopter by reverse-osmosis circulating and is greater than 10;
Step 6 is sprayed dry: add in solution admittedly containing the beta-schardinger dextrin-of thing 1-3%, stir, then carry out spraying dry, obtain faint yellow moringa oleifera leaf extractive.
Further, the extraction conditions of described ultrasonic extraction is:
Extraction solvent: 40%-60% ethanol;
Extracting temperature: 45-55 DEG C;
Raw material: Extraction solvent is 1:10-30;
Extraction time: 30-60min, extraction time: 2 times;
Extract after terminating fluid, merge extractive liquid.
Further, also comprise just reverse osmosis concentrated liquid in before step 6 and adopt 0.25-1 times of quality petroleum ether extraction, collect the step of water layer clear liquid.
Further, the pore size filter of described plate-and-frame filtration is: 100-150 order.
Further, the film core pore size filter that described microfiltration filters is 5 μm.
Adopt technique scheme, the extracting method of moringa oleifera leaf extractive of the present invention, combined by techniques such as pulverizing, supersound extraction, plate-and-frame filtration, microfiltration filtration, reverse osmosis concentration, extraction, spraying dry, guarantee finished product water solublity clarify bright while, also remain the fragrance that this product is special, the flavone polysaccharide simultaneously in extract can not loss.
Detailed description of the invention
Below in conjunction with specific embodiment, the present invention will be further described.
Embodiment 1:
Be ground into the leaf of Moringa raw material 17kg of 40-100 object powder, add 60% ethanol 340kg, temperature 50 C, supersound extraction 40min, extract after terminating, with 100 order plate-and-frame filtration, obtain an extracting solution;
In filtering residue, adding 60% ethanol 170kg, temperature 50 C, supersound extraction 40min, extract after terminating, 100 order plate-and-frame filtration, obtain secondary raffinate;
Merge extracted twice liquid, be the micro-filtrate membrane filtration of 5 μm with pore size filter, obtain microfiltration dialysis solution;
It is 10.4 that microfiltration dialysis solution adopts reverse-osmosis circulating to be concentrated to reverse osmosis concentrated liquid obscurity dense;
Containing fat-soluble pigments such as chlorophyll/carotene in concentrated solution, by the petroleum ether extraction leaf green of concentrated solution 0.5 times amount, leave standstill and collect water layer clear liquid;
Get the water layer clear liquid 5L extracted, refractive power is 10.2, adds 20g beta-schardinger dextrin-, stirs;
Spray drying tower spray is dry, and inlet temperature 185 DEG C, air-out 95 DEG C, obtains faint yellow moringa oleifera leaf extractive 411g.
Embodiment 2:
Be ground into the leaf of Moringa raw material 17kg of 40-100 object powder, add 60% ethanol 340kg, temperature 50 C, supersound extraction 40min, extract after terminating, with 100 order plate-and-frame filtration, obtain an extracting solution;
In filtering residue, adding 60% ethanol 170kg, temperature 50 C, supersound extraction 40min, extract after terminating, 100 order plate-and-frame filtration, obtain secondary raffinate;
Merge extracted twice liquid, be the micro-filtrate membrane filtration of 5 μm with pore size filter, obtain microfiltration dialysis solution;
It is 10.4 that microfiltration dialysis solution adopts reverse-osmosis circulating to be concentrated to reverse osmosis concentrated liquid obscurity dense;
Remove reverse osmosis concentrated liquid 5L, refractive power is 10.4, adds 20g beta-schardinger dextrin-, stirs;
Spray drying tower spray is dry, and inlet temperature 185 DEG C, air-out 95 DEG C, owing to not adopting petroleum ether extraction to contain a large amount of chlorophyll, obtains yellow green moringa oleifera leaf extractive 424g.
Embodiment 3:
Be ground into the leaf of Moringa raw material 17kg of 40-100 object powder, add 60% ethanol 340kg, temperature 50 C, supersound extraction 40min, extract after terminating, with 100 order plate-and-frame filtration, obtain an extracting solution;
In filtering residue, adding 60% ethanol 170kg, temperature 50 C, supersound extraction 40min, extract after terminating, 100 order plate-and-frame filtration, obtain secondary raffinate;
Merge extracted twice liquid, be the micro-filtrate membrane filtration of 5 μm with pore size filter, obtain microfiltration dialysis solution;
It is 10.4 that microfiltration dialysis solution adopts reverse-osmosis circulating to be concentrated to reverse osmosis concentrated liquid obscurity dense;
Containing fat-soluble pigments such as chlorophyll/carotene in concentrated solution, with the petroleum ether extraction fat-soluble pigment of concentrated solution 0.5 times amount, leave standstill and collect water layer clear liquid;
Get the water layer clear liquid 5L extracted, refractive power is 10.2, adds 20g beta-schardinger dextrin-, stirs;
Spray drying tower spray is dry, and inlet temperature 189 DEG C, air-out 99 DEG C, obtains faint yellow moringa oleifera leaf extractive 433g.
Embodiment 4:
Be ground into the leaf of Moringa raw material 10kg of 40-100 object powder, add 40% ethanol 100kg, temperature 45 C, supersound extraction 60min, extract after terminating, with 130 order plate-and-frame filtration, obtain an extracting solution;
In filtering residue, adding 40% ethanol 150kg, temperature 55 DEG C, supersound extraction 60min, extract after terminating, 130 order plate-and-frame filtration, obtain secondary raffinate;
Merge extracted twice liquid, be the micro-filtrate membrane filtration of 8 μm with pore size filter, obtain microfiltration dialysis solution;
It is 10.6 that microfiltration dialysis solution adopts reverse-osmosis circulating to be concentrated to reverse osmosis concentrated liquid obscurity dense;
Containing fat-soluble pigments such as chlorophyll/carotene in concentrated solution, with the petroleum ether extraction fat-soluble pigment of concentrated solution 0.25 times of quality, leave standstill and collect water layer clear liquid;
Get the water layer clear liquid 5L extracted, refractive power is 10.5, adds 15g beta-schardinger dextrin-, stirs;
Spray drying tower spray is dry, and inlet temperature 185 DEG C, air-out 95 DEG C, obtains faint yellow moringa oleifera leaf extractive 433g.
Although specifically show in conjunction with preferred embodiment and describe the present invention; but those skilled in the art should be understood that; not departing from the spirit and scope of the present invention that appended claims limits; in the form and details the present invention is made a variety of changes, be protection scope of the present invention.
Claims (4)
1. an extracting method for moringa oleifera leaf extractive, is characterized in that, comprises the steps:
Step 1 is pulverized: leaf of Moringa is ground into 40-100 object powder;
Step 2 ultrasonic extraction: adopt ultrasonic extractor to carry out supersound extraction in leaf of Moringa powder;
Step 4 is filtered: extracting solution plate-and-frame filtration; Feed liquid after plate-and-frame filtration is filtered by microfiltration, obtains microfiltration dialysis solution, and the film core pore size filter that described microfiltration filters is 3-8 μm;
Step 5 concentrates: microfiltration dialysis solution is concentrated to reverse osmosis concentrated liquid diopter by reverse-osmosis circulating and is greater than 10;
Step 6 is sprayed dry: add in solution admittedly containing the beta-schardinger dextrin-of thing 1-3%, stir, then carry out spraying dry, obtain faint yellow moringa oleifera leaf extractive.
2. the extracting method of a kind of moringa oleifera leaf extractive according to claim 1, is characterized in that, the extraction conditions of described ultrasonic extraction is:
Extraction solvent: 40%-60% ethanol;
Extracting temperature: 45-55 DEG C;
Raw material: Extraction solvent is 1:10-30;
Extraction time: 30-60min, extraction time: 2 times;
Extract after terminating fluid, merge extractive liquid,
The extracting method of a kind of moringa oleifera leaf extractive according to claim 1, is characterized in that, also comprises just reverse osmosis concentrated liquid and adopts 0.25-1 times of quality petroleum ether extraction, collect the step of water layer clear liquid in before step 6.
3. the extracting method of a kind of moringa oleifera leaf extractive according to claim 1, is characterized in that, the pore size filter of described plate-and-frame filtration is: 100-150 order.
4. the extracting method of a kind of moringa oleifera leaf extractive according to claim 1, is characterized in that, the film core pore size filter that described microfiltration filters is 5 μm.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105707377A (en) * | 2016-03-21 | 2016-06-29 | 云南圣草峰生物科技有限公司 | Processing method of moringa oleifera leaves |
| CN105769944A (en) * | 2016-03-11 | 2016-07-20 | 田洋 | Moringa oleifera extract with weight reducing effect, as well as preparation method and use of moringa oleifera extract |
| CN107184621A (en) * | 2017-05-16 | 2017-09-22 | 云南省林业科学院 | A kind of extracting method of leaf of Moringa active ingredient and its application |
| CN108096300A (en) * | 2018-01-31 | 2018-06-01 | 军事科学院军事医学研究院环境医学与作业医学研究所 | Leaf of Moringa ethanol extract and extracting method and application |
| WO2018178886A1 (en) * | 2017-03-30 | 2018-10-04 | Specchiasol S.R.L. | Method for realization of dried extracts, hydro-alcoholic extracts and protein powders derived of leaves and plant seeds of moringa oleifera. |
| CN109422818A (en) * | 2017-08-21 | 2019-03-05 | 华南农业大学 | A kind of preparation method and applications of Polysaccharides from Leaves of Moringa oleifera MLP20-1 |
| CN109602769A (en) * | 2018-12-29 | 2019-04-12 | 南昌大学第附属医院 | Moringa oleifera leaf extractive and its preparation method and application |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105769944A (en) * | 2016-03-11 | 2016-07-20 | 田洋 | Moringa oleifera extract with weight reducing effect, as well as preparation method and use of moringa oleifera extract |
| CN105707377A (en) * | 2016-03-21 | 2016-06-29 | 云南圣草峰生物科技有限公司 | Processing method of moringa oleifera leaves |
| WO2018178886A1 (en) * | 2017-03-30 | 2018-10-04 | Specchiasol S.R.L. | Method for realization of dried extracts, hydro-alcoholic extracts and protein powders derived of leaves and plant seeds of moringa oleifera. |
| CN107184621A (en) * | 2017-05-16 | 2017-09-22 | 云南省林业科学院 | A kind of extracting method of leaf of Moringa active ingredient and its application |
| CN109422818A (en) * | 2017-08-21 | 2019-03-05 | 华南农业大学 | A kind of preparation method and applications of Polysaccharides from Leaves of Moringa oleifera MLP20-1 |
| CN108096300A (en) * | 2018-01-31 | 2018-06-01 | 军事科学院军事医学研究院环境医学与作业医学研究所 | Leaf of Moringa ethanol extract and extracting method and application |
| CN109602769A (en) * | 2018-12-29 | 2019-04-12 | 南昌大学第附属医院 | Moringa oleifera leaf extractive and its preparation method and application |
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Application publication date: 20151028 |