CN104986867A - Complex microbial inoculant for improving aquaculture pond bottom mud environment and preparation method and application thereof - Google Patents
Complex microbial inoculant for improving aquaculture pond bottom mud environment and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a complex microbial inoculant for improving the aquaculture pond bottom mud environment and a preparation method and application thereof. The complex microbial inoculant is composed of bacterial powder 1, bacterial powder 2 and bacterial powder 3. The bacterial powder 1 is Thermochromatium tepidum ATCC 43061. The bacterial powder 2 is Thioalkalivibrio denitrificans DSM 13742. The bacterial powder 3 is Achromobacter denitrificans DSM 30026 and Achromobacter xylosoxidans subsp.denitrificans ATCC15173. The weight ratio of the bacterial powder 1 to the bacterial powder 2 to the bacterial powder 3 is 1-2 to 1-2 to 1-2. The complex microbial inoculant can deeply permeate into the bottom of a pond, quickly and efficiently degrade residual feeds, excrement, flora and fauna dead bodies and organic detritus depositing to the bottom of the pond, remarkably reduce ammonia nitrogen, nitrite, sulfide and other hazardous substances at the bottom of the pond, and then achieves the purposes of improving the pond bottom mud environment and purifying the water.
Description
Technical field
The present invention relates to cultivation bed mud improving technology field, fish pond, particularly relate to a kind of improvement cultivating pool bed mud environment composite fungus agent and preparation method thereof and purposes.
Background technology
Domestic in high-density pond culture mode at present, although this intensive aquaculture meets the demand of people to fishery products to a great extent, breeding environment and cultivation safety become the problem that human consumer pays close attention to the most.The utilization ratio of this high-density, intensive culture mode Middle nutrition material is on the low side, only have small part feed absorbed by cultivated animals and then form biomass, major part is then deposited to gradually in pond bottom mud by physics, chemistry or biological action together with its movement, corpse remains etc.The semi-static breeding environment of this relative closure, its self-purification capacity is relatively weak.Along with the growth of cultivation phase, pond bottom organism, nutritive substance etc. are accumulation in a large number, cause anoxic bottom cultivating pool, the harmful substance contents such as ammonia nitrogen, nitrite, sulfide sharply rise, and then overall water quality deterioration, breed bacteria pathogenic agent, this to have become in China's aquaculture evolution must faced by critical problem.
Summary of the invention
The object of the invention is to provide a kind of improvement cultivating pool bed mud environment composite fungus agent and preparation method thereof and purposes.
The technical scheme that the present invention takes is:
Improvement cultivating pool bed mud environment composite fungus agent of the present invention is made up of bacterium powder 1, bacterium powder 2 and bacterium powder 3, and described bacterium powder 1 is thermophilic purple photosynthetic bacterium Thermochromatium tepidum ATCC 43061 bacteria powder; Described bacterium powder 2 is thiobacillus denitrificans Thioalkalivibrio denitrificans DSM13742 bacteria powders; Described bacterium powder 3 is denitrification achromobacter Achromobacter denitrificans DSM 30026 and Achromobacter xylosoxidans subsp.denitrificans Achromobacter xylosoxidans subsp.denitrificans ATCC15173 bacteria powder; The weight ratio of bacterium powder 1, bacterium powder 2 and bacterium powder 3 is 1-2:1-2:1-2.
Preferred: the weight ratio of bacterium powder 1, bacterium powder 2 and bacterium powder 3 is 1:1:1.
The concrete steps of the making method of improvement cultivating pool bed mud environment composite fungus agent of the present invention are as follows:
(1) bacterium powder 1 is made:
The thermophilic purple photosynthetic bacterium Thermochromatium tepidum ATCC43061 strain cultured solution 10L of mid log phase is linked in 1000L fermentor tank and cultivates, culture condition is: yeast powder 3g/L, sodium bicarbonate 1.5g/L, sodium acetate 3.5g/L, sour hydrogen dipotassium 0.8g/L, sodium-chlor 5.5g/L, magnesium chloride hexahydrate 0.5g/L, ammonium chloride 1.5g/L, Sytron 0.8g/L, initial pH7, after leavening temperature 48 DEG C, fermentation 48h, in nutrient solution, cell density can reach 1.51 ~ 2.23 × 10
10cFU/mL; Fermentation liquor 80 order metre filter, in filtrate, add the porous-starch of filtrate weight 18%, the maltodextrin of filtrate weight 15% and filtrate weigh 0.15% Povidone and 0.15% thiocarbamide, after spray-dried, add filtrate weight 6% tricalcium phosphate and do weighting agent, colony counting method detects bacterium number, obtains viable count and is about 10
10~ 10
11thermophilic purple photosynthetic bacterium Thermochromatium tepidum ATCC 43061 bacteria powder of CFU/g;
(2) bacterium powder 2 is made:
The thiobacillus denitrificans Thioalkalivibrio denitrificans DSM13742 strain cultured solution 10L of mid log phase is linked in 1000L fermentor tank and cultivates, culture condition is: five water Sulfothiorine 5.5g/L, saltpetre 3g/L, potassium primary phosphate 1.5g/L, sodium bicarbonate 2g/L, magnesium chloride hexahydrate 0.8g/L, green vitriol 0.02g/L, initial pH6.9, after leavening temperature 29.5 DEG C, fermentation 48h, in nutrient solution, cell density can reach 1.72 ~ 2.31 × 10
10cFU/mL; Fermentation liquor 80 order metre filter, in filtrate, add the porous-starch of filtrate weight 18%, the maltodextrin of filtrate weight 15% and filtrate weigh 0.15% Povidone and 0.15% thiocarbamide, after spray-dried, add filtrate weight 6% tricalcium phosphate and do weighting agent, colony counting method detects bacterium number, obtains viable count and is about 6.51 ~ 8.94 × 10
10the thiobacillus denitrificans Thioalkalivibriodenitrificans DSM13742 bacteria powder of CFU/g;
(3) bacterium powder 3 is made:
Respectively each 5L of nutrient solution of the denitrification achromobacter Achromobacter denitrificans DSM30026 bacterial strain of mid log phase and Achromobacter xylosoxidans subsp.denitrificans Achromobacter xylosoxidanssubsp.denitrificans ATCC 15173 bacterial strain is linked into Dual culture in 1000L fermentor tank, culture condition is: ammonium chloride 1.5g/L, dipotassium hydrogen phosphate 1.5g/L, sodium-chlor 0.8g/L, Repone K 0.5g/L, magnesium sulfate heptahydrate 0.5g/L, ferric sulfate 0.002g/L, calcium chloride 0.015g/L, trace element mixing solutions 1mL, consist of: boric acid 55mg/L, seven water manganous sulfate 40mg/L, Zinc Sulphate Heptahydrate 40mg/L, cupric sulfate pentahydrate 42mg/L, four water ammonium molybdate 35mg/L, cobalt nitrate hexahydrate 28mg/L, initial pH7, after leavening temperature 26 DEG C, fermentation 48h, in nutrient solution, cell density maintains 1.52 ~ 1.85 × 10
10cFU/mL, wherein Achromobacter denitrificans DSM 30026 is 7.97 ~ 9.14 × 10
9cFU/g and Achromobacter xylosoxidans subsp.denitrificans ATCC 15173 is 8.23 ~ 9.36 × 10
9cFU/g, fermentation liquor 80 order metre filter, in filtrate, add the porous-starch of filtrate weight 18%, the maltodextrin of filtrate weight 15% and filtrate weigh 0.15% Povidone and 0.15% thiocarbamide, after spray-dried, add filtrate weight 6% tricalcium phosphate and do weighting agent, colony counting method detects bacterium number, and in bacterium powder, denitrification achromobacter Achromobacter denitrificans DSM 30026 is 5.64 ~ 6.85 × 10
10cFU/g and Achromobacter xylosoxidans subsp.denitrificans Achromobacter xylosoxidanssubsp.denitrificans ATCC 15173 is 6.32 ~ 7.16 × 10
10cFU/g,
(4) composite fungus agent is made:
By bacterium powder 1, bacterium powder 2 and bacterium powder 3 by proportioning mixing, and namely become improvement cultivation fish pond bed mud environment composite fungus agent through vacuum packaging.
In step (1), described thermophilic purple photosynthetic bacterium Thermochromatium tepidum ATCC43061 bacterial strain comes from American Type Culture preservation center, bacterium numbering ATCC 43061.
In step (2), described thiobacillus denitrificans Thioalkalivibrio denitrificans DSM13742 bacterial strain comes from German Culture Collection, bacterium numbering DSM13742.
In step (3), described denitrification achromobacter Achromobacter denitrificans DSM 30026 bacterial strain comes from German Culture Collection, bacterium numbering DSM 30026; Described Achromobacter xylosoxidans subsp.denitrificans Achromobacter xylosoxidans subsp.denitrificans ATCC 15173 bacterial strain comes from American Type Culture preservation center, bacterium numbering ATCC 15173.
Composite fungus agent of the present invention can be used for improvement cultivating pool bed mud environment, usage is: full pool Direct Uniform is shed, 1 ~ 1.5 meter of depth of water every mu use 1200 grams, the depth of water, aging pond and with serious pollution pond are with 1500 ~ 2000 grams, within every 10 days, use once, have the pharmaceutical chemicals of germicidal action and antibiotic medicine in use the first five sky of this composite fungus agent, all can not use in latter five days.
Positively effect of the present invention is as follows:
The present invention is by screening and separating multiple high performance pollution reducing viable bacteria bacterial classification from healthy aquaculture sediment of pond, composition improvement cultivating pool bed mud composite fungus agent, composite fungus agent of the present invention deeply can penetrate into pond bottom, the residual bait that pond bottom of rapidly and efficiently degrading deposits, movement, animals and plants corpse and organic debris, the objectionable impuritiess such as ammonia nitrogen, nitrite and sulfide at the bottom of remarkable reduction pond, and then reach the object improved cultivating pool bed mud environment, purify water.
Embodiment
The following examples describe in further detail of the present invention.
The invention provides a kind of improvement cultivation fish pond bed mud environment composite fungus agent and preparation method thereof.This microbiobacterial agent comprises bacterium powder 1, bacterium powder 2 and bacterium powder 3, and its making method is:
Make bacterium powder 1: by thermophilic purple photosynthetic bacterium ATCC 43061 bacterial strain (the Thermochromatium tepidum ATCC 43061 of mid log phase, buying from American Type Culture preservation center) nutrient solution 10L is linked in 1000L fermentor tank and cultivates, culture condition is: yeast powder 3 (g/L, hereafter non-specified otherwise be g/L), sodium bicarbonate 1.5, sodium acetate 3.5, acid hydrogen dipotassium 0.8, sodium-chlor 5.5, magnesium chloride hexahydrate 0.5, ammonium chloride 1.5, Sytron 0.8, initial pH7, leavening temperature 48 DEG C, after fermentation 48h, in nutrient solution, cell density can reach 1.51 ~ 2.23 × 10
10cFU/mL, fermentation liquor 80 order metre filter, in filtrate, add the porous-starch of filtrate weight 18%, the maltodextrin of filtrate weight 15% and filtrate weigh 0.15% Povidone and 0.15% thiocarbamide, after spray-dried, add filtrate weight 6% tricalcium phosphate and do weighting agent, colony counting method detects bacterium number, obtains viable count and is about 10
10~ 10
11thermophilic purple photosynthetic bacterium Thermochromatium tepidum ATCC 43061 bacteria powder of CFU/g.
Make bacterium powder 2: by thiobacillus denitrificans DSM13742 bacterial strain (the Thioalkalivibriodenitrificans DSM13742 of mid log phase, buying from German Culture Collection) nutrient solution 10L is linked in 1000L fermentor tank and cultivates, culture condition is: five water Sulfothiorine 5.5 (g/L, hereafter non-specified otherwise be g/L), saltpetre 3, potassium primary phosphate 1.5, sodium bicarbonate 2, magnesium chloride hexahydrate 0.8, green vitriol 0.02, initial pH6.9, leavening temperature 29.5 DEG C, after fermentation 48h, in nutrient solution, cell density can reach 1.72 ~ 2.31 × 10
10cFU/mL, fermentation liquor 80 order metre filter, in filtrate, add the porous-starch of filtrate weight 18%, the maltodextrin of filtrate weight 15% and filtrate weigh 0.15% Povidone and 0.15% thiocarbamide, after spray-dried, add filtrate weight 6% tricalcium phosphate and do weighting agent, colony counting method detects bacterium number, obtains viable count and is about 6.51 ~ 8.94 × 10
10the thiobacillus denitrificans Thioalkalivibrio denitrificansDSM13742 bacteria powder of CFU/g.
Make bacterium powder 3: respectively by denitrification achromobacter DSM 30026 bacterial strain (the Achromobacter denitrificans DSM 30026 of mid log phase, buy from German Culture Collection) and Achromobacter xylosoxidans subsp.denitrificans ATCC 15173 (Achromobacter xylosoxidanssubsp.denitrificans ATCC 15173, buy from American Type Culture preservation center) each 5L of nutrient solution be linked into Dual culture in 1000L fermentor tank, culture condition is: ammonium chloride 1.5 (g/L, hereafter non-specified otherwise be g/L), dipotassium hydrogen phosphate 1.5, sodium-chlor 0.8, Repone K 0.5, magnesium sulfate heptahydrate 0.5, ferric sulfate 0.002, calcium chloride 0.015, trace element mixing solutions 1mL, consist of (mg/L): boric acid 55, seven water manganous sulfates 40, Zinc Sulphate Heptahydrate 40, cupric sulfate pentahydrate 42, four water ammonium molybdates 35, cobalt nitrate hexahydrate 28, initial pH7, after leavening temperature 26 DEG C, fermentation 48h, in nutrient solution, cell density maintains 1.52 ~ 1.85 × 10
10cFU/mL.Wherein Achromobacter denitrificans DSM 30026 is 7.97 ~ 9.14 × 10
9cFU/g and Achromobacter xylosoxidans subsp.denitrificans ATCC 15173 is 8.23 ~ 9.36 × 10
9cFU/g, fermentation liquor 80 order metre filter, in filtrate, add the porous-starch of filtrate weight 18%, the maltodextrin of filtrate weight 15% and filtrate weigh 0.15% Povidone and 0.15% thiocarbamide, after spray-dried, add filtrate weight 6% tricalcium phosphate and do weighting agent, colony counting method detects bacterium number, and in bacterium powder, denitrification achromobacter DSM 30026 is 5.64 ~ 6.85 × 10
10cFU/g and Achromobacter xylosoxidans subsp.denitrificans ATCC 15173 is 6.32 ~ 7.16 × 10
10cFU/g.
Make composite fungus agent: by bacterium powder 1, bacterium powder 2 and bacterium powder 3 by proportioning mixing, and namely become improvement cultivation fish pond bed mud environment composite fungus agent through vacuum packaging.
Embodiment 1
Namely bacterium powder 1, bacterium powder 2 and bacterium powder 3 according to 1:2:1 (weight ratio) mixing, and become improvement cultivating pool bed mud environment composite fungus agent through vacuum packaging.
Embodiment 2
Namely bacterium powder 1, bacterium powder 2 and bacterium powder 3 according to 2:1:2 (weight ratio) mixing, and become improvement cultivating pool bed mud environment composite fungus agent through vacuum packaging.
Embodiment 3
Namely bacterium powder 1, bacterium powder 2 and bacterium powder 3 according to 1:1:1 (weight ratio) mixing, and become improvement cultivating pool bed mud environment composite fungus agent through vacuum packaging.
The composite fungus agent prepared by embodiment 1-3 carries out the improvement cultivating fish pond bed mud combinational environment:
Used once according to every 10 days, one meter of depth of water every mu uses composite fungus agent 1200 grams, and Direct Uniform is shed to cultivating pool.
Use that ammonia nitrogen before composite fungus agent process is 0.9mg/L, nitrite is 0.4mg/L, after using composite fungus agent, ammonia nitrogen is 0.05mg/L, nitrite is 0.02mg/L, and the degradation rate of ammonia nitrogen and nitrite reaches more than 94%.
Although illustrate and describe embodiments of the invention, for the ordinary skill in the art, be appreciated that and can carry out multiple change, amendment, replacement and modification to these embodiments without departing from the principles and spirit of the present invention, scope of the present invention is by claims and equivalents thereof.
Claims (8)
1. an improvement cultivating pool bed mud environment composite fungus agent, it is characterized in that: described composite fungus agent is made up of bacterium powder 1, bacterium powder 2 and bacterium powder 3, described bacterium powder 1 is thermophilic purple photosynthetic bacterium Thermochromatium tepidumATCC 43061 bacteria powder; Described bacterium powder 2 is thiobacillus denitrificans Thioalkalivibrio denitrificansDSM13742 bacteria powders; Described bacterium powder 3 is denitrification achromobacter Achromobacter denitrificans DSM30026 and Achromobacter xylosoxidans subsp.denitrificans Achromobacter xylosoxidans subsp.denitrificansATCC 15173 bacteria powder; The weight ratio of bacterium powder 1, bacterium powder 2 and bacterium powder 3 is 1-2:1-2:1-2.
2. improvement cultivating pool bed mud environment composite fungus agent as claimed in claim 1, is characterized in that: the weight ratio of bacterium powder 1, bacterium powder 2 and bacterium powder 3 is 1:1:1.
3. make a method for improvement cultivating pool bed mud environment composite fungus agent as claimed in claim 1 or 2, it is characterized in that: the concrete steps of described making method are as follows:
(1) bacterium powder 1 is made:
The thermophilic purple photosynthetic bacterium Thermochromatium tepidum ATCC 43061 strain cultured solution 10L of mid log phase is linked in 1000L fermentor tank and cultivates, culture condition is: yeast powder 3g/L, sodium bicarbonate 1.5g/L, sodium acetate 3.5g/L, sour hydrogen dipotassium 0.8g/L, sodium-chlor 5.5g/L, magnesium chloride hexahydrate 0.5g/L, ammonium chloride 1.5g/L, Sytron 0.8g/L, initial pH7, after leavening temperature 48 DEG C, fermentation 48h, in nutrient solution, cell density can reach 1.51 ~ 2.23 × 10
10cFU/mL; Fermentation liquor 80 order metre filter, in filtrate, add the porous-starch of filtrate weight 18%, the maltodextrin of filtrate weight 15% and filtrate weigh 0.15% Povidone and 0.15% thiocarbamide, after spray-dried, add filtrate weight 6% tricalcium phosphate and do weighting agent, colony counting method detects bacterium number, obtains viable count and is about 10
10~ 10
11thermophilic purple photosynthetic bacterium Thermochromatiumtepidum ATCC 43061 bacteria powder of CFU/g;
(2) bacterium powder 2 is made:
The thiobacillus denitrificans Thioalkalivibrio denitrificans DSM13742 strain cultured solution 10L of mid log phase is linked in 1000L fermentor tank and cultivates, culture condition is: five water Sulfothiorine 5.5g/L, saltpetre 3g/L, potassium primary phosphate 1.5g/L, sodium bicarbonate 2g/L, magnesium chloride hexahydrate 0.8g/L, green vitriol 0.02g/L, initial pH6.9, after leavening temperature 29.5 DEG C, fermentation 48h, in nutrient solution, cell density can reach 1.72 ~ 2.31 × 10
10cFU/mL; Fermentation liquor 80 order metre filter, in filtrate, add the porous-starch of filtrate weight 18%, the maltodextrin of filtrate weight 15% and filtrate weigh 0.15% Povidone and 0.15% thiocarbamide, after spray-dried, add filtrate weight 6% tricalcium phosphate and do weighting agent, colony counting method detects bacterium number, obtains viable count and is about 6.51 ~ 8.94 × 10
10the thiobacillus denitrificans Thioalkalivibrio denitrificans DSM13742 bacteria powder of CFU/g;
(3) bacterium powder 3 is made:
Respectively each 5L of nutrient solution of denitrification achromobacter Achromobacter denitrificans DSM 30026 bacterial strain of mid log phase and Achromobacter xylosoxidans subsp.denitrificans Achromobacter xylosoxidans subsp.denitrificansATCC 15173 bacterial strain is linked into Dual culture in 1000L fermentor tank, culture condition is: ammonium chloride 1.5g/L, dipotassium hydrogen phosphate 1.5g/L, sodium-chlor 0.8g/L, Repone K 0.5g/L, magnesium sulfate heptahydrate 0.5g/L, ferric sulfate 0.002g/L, calcium chloride 0.015g/L, trace element mixing solutions 1mL, consist of: boric acid 55mg/L, seven water manganous sulfate 40mg/L, Zinc Sulphate Heptahydrate 40mg/L, cupric sulfate pentahydrate 42mg/L, four water ammonium molybdate 35mg/L, cobalt nitrate hexahydrate 28mg/L, initial pH7, after leavening temperature 26 DEG C, fermentation 48h, in nutrient solution, cell density maintains 1.52 ~ 1.85 × 10
10cFU/mL, wherein Achromobacter denitrificans DSM 30026 is 7.97 ~ 9.14 × 10
9cFU/g and Achromobacter xylosoxidans subsp.denitrificans ATCC 15173 is 8.23 ~ 9.36 × 10
9cFU/g, fermentation liquor 80 order metre filter, in filtrate, add the porous-starch of filtrate weight 18%, the maltodextrin of filtrate weight 15% and filtrate weigh 0.15% Povidone and 0.15% thiocarbamide, after spray-dried, add filtrate weight 6% tricalcium phosphate and do weighting agent, colony counting method detects bacterium number, and in bacterium powder, denitrification achromobacter Achromobacter denitrificans DSM 30026 is 5.64 ~ 6.85 × 10
10cFU/g and Achromobacter xylosoxidans subsp.denitrificans Achromobacter xylosoxidans subsp.denitrificans ATCC 15173 is 6.32 ~ 7.16 × 10
10cFU/g,
(4) composite fungus agent is made:
By bacterium powder 1, bacterium powder 2 and bacterium powder 3 by proportioning mixing, and namely become improvement cultivation fish pond bed mud environment composite fungus agent through vacuum packaging.
4. making method as claimed in claim 3, it is characterized in that: in step (1), described thermophilic purple photosynthetic bacterium Thermochromatium tepidum ATCC 43061 bacterial strain comes from American Type Culture preservation center, bacterium numbering ATCC 43061.
5. making method as claimed in claim 3, it is characterized in that: in step (2), described thiobacillus denitrificans Thioalkalivibrio denitrificans DSM13742 bacterial strain comes from German Culture Collection, bacterium numbering DSM13742.
6. making method as claimed in claim 3, it is characterized in that: in step (3), described denitrification achromobacter Achromobacter denitrificans DSM 30026 bacterial strain comes from German Culture Collection, bacterium numbering DSM 30026; Described Achromobacter xylosoxidans subsp.denitrificans Achromobacter xylosoxidanssubsp.denitrificans ATCC 15173 bacterial strain comes from American Type Culture preservation center, bacterium numbering ATCC 15173.
7. composite fungus agent as claimed in claim 1 or 2 is for improveing the purposes of cultivating pool bed mud environment.
8. purposes as claimed in claim 7, it is characterized in that: the usage of described composite fungus agent is: full pool Direct Uniform is shed, 1 ~ 1.5 meter of depth of water every mu use 1200 grams, the depth of water, aging pond and with serious pollution pond are with 1500 ~ 2000 grams, within every 10 days, use once, have the pharmaceutical chemicals of germicidal action and antibiotic medicine in use the first five sky of this composite fungus agent, all can not use in latter five days.
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| CN107010731A (en) * | 2017-04-27 | 2017-08-04 | 卢松 | The handling process of high concentration starch water |
| CN107601678A (en) * | 2017-09-26 | 2018-01-19 | 惠州学院 | A kind of cities and towns black and odorous water and sediment in-situ quickly administer material |
| CN110699291A (en) * | 2019-11-04 | 2020-01-17 | 浙江树人学院（浙江树人大学） | Achromobacter xylosoxidans with sulfide degradation performance and application thereof |
| CN113651507A (en) * | 2021-09-14 | 2021-11-16 | 中国科学院南京土壤研究所 | Method for removing heavy metals in livestock and poultry manure/biogas residues and EKR-PRB coupling device |
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| CN102583775A (en) * | 2012-02-14 | 2012-07-18 | 福建微水环保技术有限公司 | Composite microorganism preparation and application thereof in waste water treatment |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106434617A (en) * | 2016-08-30 | 2017-02-22 | 武汉沃田生态科技有限公司 | Thiobacillus microcapsule inoculant used for water purification and preparing method thereof |
| CN107010731A (en) * | 2017-04-27 | 2017-08-04 | 卢松 | The handling process of high concentration starch water |
| CN107010731B (en) * | 2017-04-27 | 2020-02-07 | 内蒙古阜丰生物科技有限公司 | Treatment process of high-concentration starch wastewater |
| CN107601678A (en) * | 2017-09-26 | 2018-01-19 | 惠州学院 | A kind of cities and towns black and odorous water and sediment in-situ quickly administer material |
| CN110699291A (en) * | 2019-11-04 | 2020-01-17 | 浙江树人学院（浙江树人大学） | Achromobacter xylosoxidans with sulfide degradation performance and application thereof |
| CN110699291B (en) * | 2019-11-04 | 2021-04-16 | 浙江树人学院（浙江树人大学） | Achromobacter xylosoxidans with sulfide degradation performance and application thereof |
| CN113651507A (en) * | 2021-09-14 | 2021-11-16 | 中国科学院南京土壤研究所 | Method for removing heavy metals in livestock and poultry manure/biogas residues and EKR-PRB coupling device |
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