CN104974198A - Process of extracting tannin from pine bark - Google Patents
Process of extracting tannin from pine bark Download PDFInfo
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- CN104974198A CN104974198A CN201510329287.7A CN201510329287A CN104974198A CN 104974198 A CN104974198 A CN 104974198A CN 201510329287 A CN201510329287 A CN 201510329287A CN 104974198 A CN104974198 A CN 104974198A
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- tannin
- cortex pini
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Abstract
The present invention discloses a process of extracting tannin from pine bark. The method comprises the following steps of: (1) pre-treatment: grinding naturally dried pine bark, and sieving the ground pine bark by using a 100 mesh sieve, so as to obtain pine bark powder for later use; (2) microwave-assisted extraction: weighing out pine bark powder, putting the powder in a round bottom flask with a reflux condenser, adding an extraction liquid, then putting the round bottom flask in a microwave reactor, performing microwave-assisted extraction for 1 minute at the temperature of 45 DEG C with microwave power being 80-85 W, thus obtaining a mixed liquid extract; and (3) purification: cooling the obtained mixed liquid extract to the room temperature, filtering the mixed liquid extract, concentrating the filtrate in vacuum at the temperature of 40 DEG C, and drying the filtrate in vacuum until the mass remains constant, thereby obtaining a tannin extract of white birch. The process of extracting tannin from pine bark provided by the invention has the advantages of being simple in operation and high in efficiency, and provides a novel approach for pine bark utilization and improves the added value of pine bark.
Description
Technical field
The present invention relates to technical field of plant extraction, especially relate to a kind of technique extracting tannin from Cortex Pini.
Background technology
Containing many kinds of substance in Cortex Pini, from Cortex Pini, extracting substance is of common occurrence, the domestic research to Cortex Pini Massonianae extract at present focuses on the material anthocyanidin (pycnogenols oligomer) (OPCs) of the resistance of oxidation in Cortex Pini Massonianae extract, this material resistance of oxidation is 20 times of vitamin C, 50 times of vitamin E.OPCs can effectively stranglehold base effect as famous antioxidant, and free radical is in degenerative disease, cardiovascular disorder, blurred vision, and the aspects such as sunburn and early ageing play an important role.
But also can extract containing many kinds of substance in Cortex Pini, just containing more tannin in Cortex Pini, tannin (Tannins) is also known as Weibull, tannin, tannic acid.It is extensively present in the leaf of plant, fruit, the position such as root and skin, is also that trees are subject to insect infestations and the main component of the insect gall generated; Be mainly used in the fields such as medicine, food, process hides, household chemicals, water treatment.Tannin is the compound of polyphenol camber polymerization, and be yellow or the amorphous bulky powder of brown color, color deepens gradually in atmosphere, has stronger water absorbability; Be insoluble to ether, benzene, chloroform, soluble in water, ethanol, acetone, the aqueous solution has astringent taste.The chemical composition more complicated of tannin, is roughly divided into two kinds: one is hydrolysable tannins, be polynary phenolic acid and sugar mainly with the compound that ester bond is formed, as gallic acid tannin and ellagic acid tannin; One is condensed tannin, and important composition composition is Flavonol and derivative thereof, tannin as wooden in heavily fortified point, chaste tree Tannins From Barks and larch tannin.The tannin of specific molecular structure has physiologically active, can have an effect with the protein in organism, polysaccharide, nucleic acid etc.The effect of tannin to human body has duality, the excessive effect that can produce anti-nutrition of the tannin in food, as reduced the nutritive value of protein, affecting effective utilization of VA and Vb12.Tannin is for oral administration on traditional medicine can control gastrointestinal hemorrhage and only dysentery, to protect from infection inflammation outward for local hemostasis and protecting wound surface; Research in recent years find tannin also have inhibitory anti-virus, antianaphylaxis, anti-oxidant and delay senility, the effect such as preventing cardiovascular disease, antitumor and Promote immunity.Tannin can be used as functional ingredient for making healthcare products in food service industry, produces foodstuff additive, flavour agent etc.; Convergence, sun-proof, whitening, crease-resistant, moisturizing, the effect such as anticorrosion is mainly played in makeup; In tanning industry, tannin is the main component of tannin extract.Therefore, utilize Cortex Pini extraction tanning matter can increase the value added of Cortex Pini, there is important economic benefit and social benefit.
Summary of the invention
In order to realize object of the present invention, the invention provides a kind of technique extracting tannin from Cortex Pini, method of the present invention has easy and simple to handle, extraction efficiency advantages of higher, and the utilization for Cortex Pini provides a kind of new approach, improves the value added of Cortex Pini.
To achieve these goals, the technical solution used in the present invention is as follows:
Extract a technique for tannin from Cortex Pini, this technique comprises the following steps:
(1) pre-treatment: pulverized 100 mesh sieves after air-dry for Cortex Pini natural drying, obtain Cortex Pini powder, for subsequent use;
(2) microwave extraction: take Cortex Pini powder for subsequent use, be placed in the round-bottomed flask of band reflux condensing tube, then extracting solution is added, again round bottom flask is entered in microwave reactor, be 80-85W at microwave power, temperature is carry out microwave extraction 1min under 45 DEG C of conditions, obtains mixed extract;
(3) purifying: will obtain carrying out suction filtration after mixed extract is cooled to normal temperature, after vacuum concentration at filtrate 40 DEG C, vacuum-drying is to constant mass at 60 DEG C, white birch tanning extract.
In the present invention, as further illustrating, extracting solution described in described step (2) and pine tree powder are 1:20-30 according to mass ratio.
In the present invention, as further illustrating, described extracting solution is according to ethanol: acetone: quality is than the mixed solution for 10-15:8-10:0.3-0.5.
In the present invention, as further illustrating, the specification of described round-bottomed flask is 250mL.
The invention has the beneficial effects as follows:
1. the present invention carries out the tanning matter in microwave extraction Cortex Pini with ethanol, acetone, water mixed liquid position Extraction solvent, by allocating the best factor such as solid-liquid ratio, Extracting temperature, microwave power, extraction time, shorten extraction time, increase the extraction yield of effective constituent, and compared with extracting with tradition heat, microwave radiation exaraction has that the time is short, extraction efficiency is high.
2. the present invention has technique simply, easy handling, extraction yield advantages of higher.
3. tannin tannin has hemostasis, suppresses microorganism, antianaphylaxis, anti-mutation, anticancer, antitumor, the anti-ageing effect of waiting for a long time, method provided by the invention extracts tannin can give full play to the economic worth that excavation Cortex Pini is felled and industrial wood waste is potential, for increasing peasant income provide a kind of new approach.
Embodiment
Further illustrate the present invention below by specific embodiment, be easier to make advantages and features of the invention be understood, it should be understood that embodiments of the invention are only used for the present invention, instead of limitation of the present invention.
Embodiment 1
Extract a technique for tannin from Cortex Pini, this technique comprises the following steps:
(1) pre-treatment: pulverized 100 mesh sieves after air-dry for Cortex Pini natural drying, obtain Cortex Pini powder, for subsequent use;
(2) microwave extraction: take Cortex Pini powder for subsequent use, be placed in the 250mL round-bottomed flask of band reflux condensing tube, then add according to ethanol: acetone: quality is extracting solution than the mixed solution for 10:8:0.3, wherein, extracting solution and pine tree powder are 1:20 according to mass ratio, then enter in microwave reactor by round bottom flask, are 80W at microwave power, temperature is carry out microwave extraction 1min under 45 DEG C of conditions, obtains mixed extract;
(3) purifying: will obtain carrying out suction filtration after mixed extract is cooled to normal temperature, after vacuum concentration at filtrate 40 DEG C, vacuum-drying is to constant mass at 60 DEG C, white birch tanning extract.
Through calculating, the present embodiment last gained tannin total amount is 110.05mg/100g.
Embodiment 2
Extract a technique for tannin from Cortex Pini, this technique comprises the following steps:
(1) pre-treatment: pulverized 100 mesh sieves after air-dry for Cortex Pini natural drying, obtain Cortex Pini powder, for subsequent use;
(2) microwave extraction: take Cortex Pini powder for subsequent use, be placed in the 250mL round-bottomed flask of band reflux condensing tube, then add according to ethanol: acetone: quality is extracting solution than the mixed solution for 3:2:0.1, wherein, extracting solution and pine tree powder are 1:30 according to mass ratio, then enter in microwave reactor by round bottom flask, are 85W at microwave power, temperature is carry out microwave extraction 1min under 45 DEG C of conditions, obtains mixed extract;
(3) purifying: will obtain carrying out suction filtration after mixed extract is cooled to normal temperature, after vacuum concentration at filtrate 40 DEG C, vacuum-drying is to constant mass at 60 DEG C, white birch tanning extract.
Through calculating, the present embodiment last gained tannin total amount is 112.35mg/100g.
Embodiment 3
Extract a technique for tannin from Cortex Pini, this technique comprises the following steps:
(1) pre-treatment: pulverized 100 mesh sieves after air-dry for Cortex Pini natural drying, obtain Cortex Pini powder, for subsequent use;
(2) microwave extraction: take Cortex Pini powder for subsequent use, be placed in the 250mL round-bottomed flask of band reflux condensing tube, then add according to ethanol: acetone: quality is extracting solution than the mixed solution for 13:9:0.4, wherein, extracting solution and pine tree powder are 1:25 according to mass ratio, then enter in microwave reactor by round bottom flask, are 81W at microwave power, temperature is carry out microwave extraction 1min under 45 DEG C of conditions, obtains mixed extract;
(3) purifying: will obtain carrying out suction filtration after mixed extract is cooled to normal temperature, after vacuum concentration at filtrate 40 DEG C, vacuum-drying is to constant mass at 60 DEG C, white birch tanning extract.
Through calculating, the present embodiment last gained tannin total amount is 119.52mg/100g.
Embodiment 4
Extract a technique for tannin from Cortex Pini, this technique comprises the following steps:
(1) pre-treatment: pulverized 100 mesh sieves after air-dry for Cortex Pini natural drying, obtain Cortex Pini powder, for subsequent use;
(2) microwave extraction: take Cortex Pini powder for subsequent use, be placed in the 250mL round-bottomed flask of band reflux condensing tube, then add according to ethanol: acetone: quality is extracting solution than the mixed solution for 14:9:0.3, wherein, extracting solution and pine tree powder are 1:29 according to mass ratio, then enter in microwave reactor by round bottom flask, are 84W at microwave power, temperature is carry out microwave extraction 1min under 45 DEG C of conditions, obtains mixed extract;
(3) purifying: will obtain carrying out suction filtration after mixed extract is cooled to normal temperature, after vacuum concentration at filtrate 40 DEG C, vacuum-drying is to constant mass at 60 DEG C, white birch tanning extract.
Through calculating, the present embodiment last gained tannin total amount is 120.11mg/100g.
Cortex Pini extraction tannin extraction process provided by the present invention is simple, cost is low, extraction yield is high, and show that the tannin content of this finished product is high through efficient liquid phase chromatographic analysis, purity is high, and has higher biological activity, has very high using value.
Claims (4)
1. extract a technique for tannin from Cortex Pini, it is characterized in that, this technique comprises the following steps:
(1) pre-treatment: pulverized 100 mesh sieves after air-dry for Cortex Pini natural drying, obtain Cortex Pini powder, for subsequent use;
(2) microwave extraction: take Cortex Pini powder for subsequent use, be placed in the round-bottomed flask of band reflux condensing tube, then extracting solution is added, again round bottom flask is entered in microwave reactor, be 80-85W at microwave power, temperature is carry out microwave extraction 1min under 45 DEG C of conditions, obtains mixed extract;
(3) purifying: will obtain carrying out suction filtration after mixed extract is cooled to normal temperature, after vacuum concentration at filtrate 40 DEG C, vacuum-drying is to constant mass at 60 DEG C, white birch tanning extract.
2. a kind of technique extracting tannin from Cortex Pini according to claim 1, is characterized in that: extracting solution described in described step (2) and pine tree powder are 1:20-30 according to mass ratio.
3. a kind of technique extracting tannin from Cortex Pini according to claim 1, is characterized in that: described extracting solution is according to ethanol: acetone: quality is than the mixed solution for 10-15:8-10:0.3-0.5.
4. a kind of technique extracting tannin from Cortex Pini according to claim 3, is characterized in that: the specification of described round-bottomed flask is 250mL.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109403025A (en) * | 2018-10-22 | 2019-03-01 | 太和县瑞丝源发制品有限公司 | A kind of production method of wig nursing Essence |
| CN112458132A (en) * | 2020-11-17 | 2021-03-09 | 汕头大学 | Method for preparing multi-element antibacterial probiotic by using single raw material |
Citations (5)
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| AU8166387A (en) * | 1985-12-23 | 1988-03-17 | Commonwealth Scientific And Industrial Research Organisation | Tannin extraction and processing |
| CN101161664A (en) * | 2006-10-13 | 2008-04-16 | 许连科 | Method for extracting tannin from lichee epidermis and uses thereof |
| CN101613383A (en) * | 2009-08-03 | 2009-12-30 | 五峰赤诚生物科技有限公司 | A kind of method of from persimmon, producing persimmon tannin |
| CN102050844A (en) * | 2010-11-11 | 2011-05-11 | 梁锋 | Method for extracting tannin by using eucalyptus barks |
| CN102643310A (en) * | 2012-03-27 | 2012-08-22 | 中国林业科学研究院林产化学工业研究所 | Method for extracting chestnut shell tannin through microwave composite technique |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU8166387A (en) * | 1985-12-23 | 1988-03-17 | Commonwealth Scientific And Industrial Research Organisation | Tannin extraction and processing |
| CN101161664A (en) * | 2006-10-13 | 2008-04-16 | 许连科 | Method for extracting tannin from lichee epidermis and uses thereof |
| CN101613383A (en) * | 2009-08-03 | 2009-12-30 | 五峰赤诚生物科技有限公司 | A kind of method of from persimmon, producing persimmon tannin |
| CN102050844A (en) * | 2010-11-11 | 2011-05-11 | 梁锋 | Method for extracting tannin by using eucalyptus barks |
| CN102643310A (en) * | 2012-03-27 | 2012-08-22 | 中国林业科学研究院林产化学工业研究所 | Method for extracting chestnut shell tannin through microwave composite technique |
Non-Patent Citations (1)
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| 刘国成，等: "微波辅助提取云南松树皮中的缩合单宁", 《山东林业科技》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109403025A (en) * | 2018-10-22 | 2019-03-01 | 太和县瑞丝源发制品有限公司 | A kind of production method of wig nursing Essence |
| CN112458132A (en) * | 2020-11-17 | 2021-03-09 | 汕头大学 | Method for preparing multi-element antibacterial probiotic by using single raw material |
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