CN104969860A - Nandina domestica 'Fire power' tissue-culture transplanted plantlets' short sprouts cutting propagation method - Google Patents
Nandina domestica 'Fire power' tissue-culture transplanted plantlets' short sprouts cutting propagation method Download PDFInfo
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- CN104969860A CN104969860A CN201510360552.8A CN201510360552A CN104969860A CN 104969860 A CN104969860 A CN 104969860A CN 201510360552 A CN201510360552 A CN 201510360552A CN 104969860 A CN104969860 A CN 104969860A
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Abstract
The invention discloses a Nandina domestica 'Fire power' tissue-culture transplanted plantlets' short sprouts cutting propagation method, which comprises the following steps: 1) a rooting agent and a cutting medium are prepared; 2) when Nandina domestica 'Fire power' tissue-culture transplanted plantlets grow to 10-20 cm high, branches of 5-8 cm long are cut; the branches are cut into small sections of 2-4 cm long and all leaves are retained, and the small sections of branches are immersed in the prepared rooting agent for 12-15s and then inserted in a prepared medium; 3) the short sprouts cuttings are rooted in an aperture disk containing the medium and kept under the full-illumination spray condition and at the relative humidity of 85-95% and at the temperature of 20-30 DEG C; callus tissues grow from the root of the short sprouts cuttings after 15-18 days; root systems grow from the callus tissues after 25-28 days; and new buds sprout after 34 days. By cutting short sprouts cuttings from tissue-culture transplanted plantlets, excellent traits of original maternal plants are maintained, and survival rate of cottage exceeds 95%. The method provided by the invention has advantages of simple operation, low cost and high efficiency.
Description
Technical field
The present invention relates to the cuttage breeding method of a Plants, specifically, relate to a kind of Nandina domestica'Fire power'
Group training transplant survival seedling single node cutting propagation method, belongs to flowers and trees seedling vegetative propagation technique field.
Background technology
Nandina domestica'Fire power' (
nandina domestica' Fire power '), belonging to Berberidaceae Nandina, is the heavenly bamboo gardening new varieties of abroad cultivating.Plant is short and small, and strain shape is compact, and blade is oval or avette, its internode is ultrashort, and Nandina domestica'Fire power' starts rudiment in Jiangsu Province mid-April, grows light red young leaves, blade is long, large, and temperature more than 30 DEG C just turns green, the l2 month gradually, when temperature drops to less than 5 DEG C, day and night temperature is more than 10 DEG C, and blade starts to redden, because it is cold-resistant evergreen, do not wither through the winter, very beautiful, be suitable for doing quilt and garth environment arrange.The cottage propagation of existing propagation technique, because Nandina domestica'Fire power' plant is short and small, stipes is short, and internode only has 1/10 of common heavenly bamboo, and branch is thin and delicate, the cuttage seedling raising method that production adopts usually, and the breeding cycle is long, and reproduction rate is extremely low, the tissue culture propagating of existing propagation technique, in Nandina domestica'Fire power' Regenerated Plantlets, there are some to report, difficult and that transplanting survival rate is low problem of taking root limits the spread of Nandina domestica'Fire power', traditional method for tissue culture achieves certain success, but growth coefficient is low and growth rate becomes technical bottleneck slowly, Zhejiang Cixi cotton Science Institute research in 2002 shows that Nandina domestica'Fire power' adopts traditional method for tissue culture, squamous subculture needs 45 d, the growth coefficient of bud is only 2.5, within 2003, Shanghai City landscape research is studied and is shown rooting rate only between 30% ~ 50%, therefore adopt traditional method for tissue culture to numerous soon not breakthrough of Nandina domestica'Fire power'.The immersion liquid tissue culture systems that project seminar of Jiangsu Polytechnic College of Agriculture and Forestry in 2014 develops voluntarily, be studied from the tissue cultivation rapid breeding method of minimal medium angle to Nandina domestica'Fire power', improve method for tissue culture, shorten the reproduction coefficient of breeding cycle and raising, however still there is complicated operation in the tissue culture technique of this plant, the problems such as operating cost is high, and reproductive efficiency is on the low side.
Train transplant survival seedling single node cutting propagation method for utilizing Nandina domestica'Fire power' group and yet there are no report.
Summary of the invention
Because Nandina domestica'Fire power' tissue cultivating and seedling cost is relatively high, technical problem to be solved by this invention is to provide a kind of easy and simple to handle, low cost, high-servival rate, the efficiently transplant survival of Nandina domestica'Fire power' group training fast seedling single node cutting propagation method.
For solving the problems of the technologies described above, the invention provides Nandina domestica'Fire power' group training transplant survival seedling single node cutting propagation method, it is characterized in that, comprise the following steps:
1) prepare cutting medium and root-growing agent: by perlite and vermiculite in 4: 3 ~ 3: 2(volume ratio) ratio be hybridly prepared into cutting medium, with for subsequent use after mass concentration 3% ferrous sulfate aqueous solution spray disinfectant; Adopt indolebutyric acid (IBA) 50 ~ 150 mg and heteroauxin (IAA) 50 ~ 150 mg mixed in equal amounts, dissolve rear and pyridoxine hydrochloride (VB with the ethanol of a small amount of mass concentration 95%
6) 10 ~ 15 mg mixing, add water and be settled to 1 L, be made into root-growing agent solution, for subsequent use;
2) when the annual 6-9 month, when Nandina domestica'Fire power' group training transplant survival seedling grows to 10 ~ 20cm height, long 5 ~ 8cm the branch of clip, branch is cut into again the segment of 2 ~ 4 cm, each short fringe cutting retains whole blade, dip 12 ~ 15s in the root-growing agent prepared after, insert in the matrix of preparation;
3) by short fringe cutting cuttage containing matrix cave dish in, then under full exposure spray condition, keep relative moisture 85% ~ 95%, temperature controls between 20 ~ 30 DEG C, within 15 ~ 18 days, short fringe cutting base portion starts to produce callus, within 25 ~ 28 days, go out root system from callus director, after 34 days, grow sprouting.
The beneficial effect that the present invention reaches: the present invention adopts tissue cultures transplant survival seedling, cutting is done by the short fringe of its tender stem segments of continuous clip, cuttage is carried out after plant growth regulator, vitamin mixed solution are as root-growing agent process, the merit of former plant can be kept, effectively can solve tissue cultures cost high problem simultaneously, method survival rate of the present invention is high, and cost is low, and efficiency is high.This method can cultivate a large amount of high quality seedling in a short time, expands the breeding production scale of Nandina domestica'Fire power', accelerates the promotion rate of high quality seedling.
Embodiment
embodiment 1
1) prepare cutting medium and root-growing agent: by perlite and vermiculite in 4: 3(volume ratio) ratio be hybridly prepared into cutting medium, with for subsequent use after mass concentration 3% ferrous sulfate aqueous solution spray disinfectant; Adopt indolebutyric acid (IBA) 50 mg and heteroauxin (IAA) 150 mg mixed in equal amounts, dissolve rear and pyridoxine hydrochloride (VB with the ethanol of a small amount of mass concentration 95%
6) 10 mg mixing, add water and be settled to 1 L, be made into root-growing agent solution, for subsequent use;
2) when the annual 6-9 month, when Nandina domestica'Fire power' plantlet in vitro grows to 10cm height, the long 5 cm branches of clip, branch is cut into again the segment of 2 cm, each short fringe cutting retains whole blade, after 12s is dipped in the morphology lower end of short fringe cutting in the root-growing agent prepared, by the matrix of 1/3 of short fringe cutting dark partial insertion preparation;
3) by short fringe cutting cuttage containing matrix cave dish in, matrix cave dish in height be 10cm.Then under full exposure spray condition, keep relative moisture 85% ~ 95%, temperature controls between 20 ~ 30 DEG C, within 15 days, short fringe cutting base portion starts to produce callus, within 25 days, go out root system from callus director, sprouting is grown after 34 days, nutrient solution is used to spray cuttage seedling, growth promoting effects, described nutrient solution consists of: each amounts of components when preparing 1 L solution is: ammonium nitrate 0.6g, potassium nitrate 0.7g, calcium chloride 0.24g, magnesium sulfate 0.38 g, potassium dihydrogen phosphate 0.8g, gibberellin 5.0mg.The present embodiment cuttage survival rate 96.8%.
embodiment 2
1) prepare cutting medium and root-growing agent: by perlite and vermiculite in 3: 2(volume ratio) ratio be hybridly prepared into cutting medium, with for subsequent use after mass concentration 3% ferrous sulfate aqueous solution spray disinfectant; Adopt indolebutyric acid (IBA) 150 mg and heteroauxin (IAA) 50 mg mixed in equal amounts, dissolve rear and pyridoxine hydrochloride (VB with the ethanol of a small amount of mass concentration 95%
6) 15 mg mixing, add water and be settled to 1 L, be made into rooting promoter solution, for subsequent use;
2) when the annual 6-9 month, when Nandina domestica'Fire power' group training transplant survival seedling grows to 20 cm height, the long 8 cm branches of clip, branch is cut into again the segment of 4 cm, each short fringe cutting retains whole blade, after 15 s are dipped in the morphology lower end of short fringe cutting in the root-growing agent prepared, by the matrix of 1/3 of short fringe cutting dark partial insertion preparation;
3) by short fringe cutting cuttage containing matrix cave dish in, matrix cave dish in height be 10cm.Then under full exposure spray condition, keep relative moisture 85% ~ 95%, temperature controls between 20 ~ 30 DEG C, within 18 days, short fringe cutting base portion starts to produce callus, within 28 days, go out root system from callus director, after 34 days, grow sprouting, use nutrient solution to spray cuttage seedling, growth promoting effects, described nutrient solution consists of: ammonium nitrate 0.8g, potassium nitrate 0.8 g, calcium chloride 0.36 g, magnesium sulfate 0.46g, potassium dihydrogen phosphate 1.2 g, gibberellin 7mg.The present embodiment cuttage survival rate 95.2%.
Below disclose the present invention with preferred embodiment, so it is not intended to limiting the invention, and all employings are equal to replacement or the technical scheme that obtains of equivalent transformation mode, all drop within protection scope of the present invention.
Claims (5)
1. Nandina domestica'Fire power' group training transplant survival seedling single node cutting propagation method, is characterized in that: comprise the following steps:
1) prepare cutting medium and root-growing agent: by perlite and vermiculite by volume 4: 3 ~ 3: 2 ratio be hybridly prepared into cutting medium, with for subsequent use after mass concentration 3% ferrous sulfate aqueous solution spray disinfectant; Adopt indolebutyric acid 50 ~ 150 mg and heteroauxin 50 ~ 150 mg mixed in equal amounts, the ethanol with a small amount of 95% mixes with pyridoxine hydrochloride 10 ~ 15 mg after dissolving, and adds water and is settled to 1 L, be made into root-growing agent solution, for subsequent use;
2) when the annual 6-9 month, when Nandina domestica'Fire power' group training transplant survival seedling grows to 10 ~ 20cm height, long 5 ~ 8cm the branch of clip, segment branch being cut into again 2 ~ 4 cm forms short fringe cutting, each short fringe cutting retains whole blade, dip 12 ~ 15s in the root-growing agent prepared after, insert in the matrix of preparation;
3) by short fringe cutting cuttage containing matrix cave dish in, then under full exposure spray condition, keep relative moisture 85% ~ 95%, temperature controls between 20 ~ 30 DEG C, within 15 ~ 18 days, short fringe cutting base portion starts to produce callus, within 25 ~ 28 days, go out root system from callus director, after 34 days, grow sprouting.
2. Nandina domestica'Fire power' group training transplant survival seedling single node cutting propagation method according to claim 1, is characterized in that: step 2) in, the morphology lower end of short fringe cutting is dipped in root-growing agent solution.
3. Nandina domestica'Fire power' group training transplant survival seedling single node cutting propagation method according to claim 1, is characterized in that: step 2) in, deeply insert 1/3 of short fringe cutting in the matrix of preparation.
4. Nandina domestica'Fire power' group training transplant survival seedling single node cutting propagation method according to claim 1, it is characterized in that: in described step 3), after growing sprouting, nutrient solution is used to spray cuttage seedling, growth promoting effects, described nutrient solution consists of: each amounts of components when preparing 1 L solution is: ammonium nitrate 0.6-0.8g, potassium nitrate 0.7-0.8 g, calcium chloride 0.24-0.36 g, magnesium sulfate 0.38-0.46g, potassium dihydrogen phosphate 0.8-1.2 g, gibberellin 5.0-7.0mg.
5. Nandina domestica'Fire power' group training transplant survival seedling single node cutting propagation method according to claim 1, it is characterized in that: in described step 3), the height of matrix in the dish of cave is 10cm.
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| CN201510360552.8A CN104969860A (en) | 2015-06-26 | 2015-06-26 | Nandina domestica 'Fire power' tissue-culture transplanted plantlets' short sprouts cutting propagation method |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110839421A (en) * | 2019-12-04 | 2020-02-28 | 江苏农林职业技术学院 | Method for promoting rooting of Nandina domestica by cutting |
| CN113875593A (en) * | 2021-10-22 | 2022-01-04 | 杭州木木生物科技有限公司 | Sugar-free tissue culture method and device for improving rooting rate of nandina domestica |
-
2015
- 2015-06-26 CN CN201510360552.8A patent/CN104969860A/en active Pending
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| 刘卫东等: "《林木种苗繁育新技术》", 31 May 2006 * |
| 杜永芹等: ""观赏林木新品种火焰南天竹的引进与繁育技术研究"", 《上海农业学报》 * |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110839421A (en) * | 2019-12-04 | 2020-02-28 | 江苏农林职业技术学院 | Method for promoting rooting of Nandina domestica by cutting |
| CN113875593A (en) * | 2021-10-22 | 2022-01-04 | 杭州木木生物科技有限公司 | Sugar-free tissue culture method and device for improving rooting rate of nandina domestica |
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