CN104962584A - Microorganism synthesis method of Fe-containing active matter and application of Fe-containing active matter in methyl orange degradation and catalyzing field - Google Patents

Abstract

The invention relates to a matter microorganism synthesis method of Fe-containing active matter and application of the Fe-containing active matter in the methyl orange degradation and catalyzing field. By means of the method, the Fe-containing active matter is prepared easily, other raw materials are cheap and easy to obtain, the method that Fe-containing containing matter is degraded through a catalyst is simple and efficient, the Fe-containing active matter can generate Fe(OH)2+, Fe(OH)2+ and Fe2(OH)24+ which are high in activity in water, and these substances are in cooperation with titanium dioxide under the action of organic acid and oxygen to generate oxygen radicals with the high oxidizing property, so that the methyl orange can be degraded thoroughly, the degradation effect is remarkable, and the method is suitable for treating sewage containing the methyl orange on a large scale.

Description

A kind of Microbe synthesis method of iron content actives and the application at methyl orange degradation catalytic field thereof

Technical field

The present invention relates to a kind of catalyzer, particularly relate to a kind of Microbe synthesis method of iron content actives and the application at methyl orange degradation catalytic field thereof, belong to dye degrades field.

Background technology

Dyestuff to make filamentary material or other materials obtain the distinct and organism of solid color, and dye soluble, in water solvent, maybe can be transformed into solution and dye, or is processed into dispersion state and is employed, and it is mainly used in the dyeing of textiles and leather.Its history is almost long equally with human history, the dyestuff of early application is all natural, and within 1856, Englishize scholar Perkin synthesizes first synthetic dyestuff mauvein (e), 20 beginnings of the century, synthetic dyestuff almost instead of natural dyestuff completely, current commercial dye oneself have kind more than 10,000.

The sorting technique of dyestuff has two kinds, and one is classify according to the chemical structure of dyestuff, and two is classify according to the application method of dyestuff, and be with basic structure similar in dye molecule for foundation, dyestuff can be divided into azoic dyestuff, anthraquinone dye and indigoids dyestuff etc.; By the tint applications property sort of dyestuff, dyestuff can be divided into matching stain, basic dyestuff and neutral dye etc.Along with the development of dyestuffs industries, the problem of environmental pollution becomes increasingly conspicuous, and a large amount of waste water how processing dyestuffs industries generation become the emphasis of people's research.

In the molecular structure of dyestuff, all have conjugated system, the dyestuff wherein containing azo-group (-N=N-) is called azoic dyestuff.Now, commercial dye more than half is containing azo-group, and the dyestuff that textile industry uses about 70% is azoic dyestuff, the azoic dyestuff that nearly 2000 various structures are different.Tropeolin-D, the one of azoic dyestuff, formal name used at school is to dimethylaminoazobenzene sodium sulfonate, and it is the acetate by p-aminophenyl sulphur diazonium salt and-DMA, in weak acid medium, coupling obtains, what coupling first obtained is red acidic methylene orange, and be called turmeric yellow, in alkalescence, turmeric yellow changes the sodium salt of tropeolin-D into, i.e. tropeolin-D (Methyl Orange, MO).Tropeolin-D is reagent conventional in chemical experiment, and the methyl orange aqueous solution of 0.1% is conventional acid base indicator, pH value color change interval 3.1 (red)-4.4 (Huang).

In the last few years, about the carinogenicity of aromatic amine azoic dyestuff receives much concern.Because some dyestuff may be transferred to the skin of people from textiles, when particularly dyefastness is not good, under the biocatalysis of bacterium, may reduction reaction be there is in the dyestuff that skin has been stained with, and discharge multiple carcinogenic aromatic amine, these carcinogenss in human body, become the risk factor of human lesion through skin diffusion, thus bring out cancer or cause allergy.

After undressed waste water from dyestuff directly discharges entered environment water body, on the one hand because dyestuff affects the transmission of light, thus affect the photosynthesis of waterplant, finally cause waterplant dead, and then cause interruption and the water hypoxia of food chain in water body, make whole water ecosystem close to collapse; On the other hand dyestuff especially azo dyes belong to and three to cause the hazardous and noxious substances of (carcinogenic, teratogenesis, mutagenesis), there is direct killing effect to the animal in water body, enter after human body can cause the malignant diseases such as human malignancies through food chain enrichment.

Coagulant sedimentation, absorption method, By Bubble-floating Method, biological process and chemical oxidization method etc. are had at present for the method containing Methyl Orange in Wastewater process.The shortcoming that these methods exist is: removal effect is not thorough, higher to environmental requirement, synthesis technique is more complicated, therefore needs the Water Pollution Problem that the process of a kind of cost-effective dye discoloration recovery technique is day by day serious badly.

Summary of the invention

The present invention is directed to the deficiency that above-mentioned prior art exists, a kind of Microbe synthesis method of iron content actives and the application at methyl orange degradation catalytic field thereof are provided.

The technical scheme that the present invention solves the problems of the technologies described above is as follows: a kind of Microbe synthesis method of iron content actives, and step is as follows:

(1) in reactor, add FeSO4 〃 7H2O and iron protoxide bacillus Acidithtobacillusferrooxidans LX5 suspension thalline, the pH regulating reaction solution with dilute sulphuric acid is 1.5 ~ 3.0;

(2) mixed solution of step (1) is placed in 28 DEG C of reciprocal shaker shaking culture 1 ~ 3 day;

(3) mixed solution after step (2) being cultivated filters, and the pH of gained throw out sulfuric acid acidation is the distilled water wash 2 ~ 3 times of 1.5 ~ 2.5, then uses distilled water wash 2 ~ 3 times, dries to obtain product.

Second object of the present invention is to provide a kind of catalyzer for tropeolin-D in degradation of sewage, comprise iron content actives, organic acid and titanium dioxide described above, its component by weight mark is counted: iron content actives 73 ~ 76%, organic acid 18 ~ 20% and titanium dioxide 5 ~ 7%.

Wherein, described organic acid is citric acid, tartrate, oxalic acid or EDTA.

3rd object of the present invention is a kind of method providing catalyzer described above for tropeolin-D of degrading, and step is as follows:

(1) get above-mentioned catalyzer 1g to add in the beaker of 1000g containing the waste water of tropeolin-D, fully mix;

(2) under the waste water mixed being placed in room temperature, Keep agitation 2h;

(3) its absorbancy is surveyed at tropeolin-D maximum absorption wavelength 463nm place, the residual content of tropeolin-D in wastewater measurement.

The invention has the beneficial effects as follows: iron content actives preparation of the present invention is simple, and other cheaper starting materials are easy to get, and catalyzer is simply efficient to the degradation method of tropeolin-D, and iron content active substance can produce active higher Fe (OH) in water ²⁺, Fe (OH) ²⁺and Fe ₂(OH) ₂ ⁴⁺, these materials work in coordination with titanium dioxide the oxygen radical producing and have strong oxidizing property under organic acid, oxygen effect, thus degraded thoroughly, and Be very effective is applicable to process large-scale containing tropeolin-D sewage.

Embodiment

Be described principle of the present invention and feature below in conjunction with example, example, only for explaining the present invention, is not intended to limit scope of the present invention.

The instrument that the embodiment of the present invention uses is T-6 series ultraviolet-visible spectrophotometer (Beijing Pu Xi general instrument Corp.).

Embodiment 1

For a catalyzer for tropeolin-D in degradation of sewage, its component by weight mark is counted: iron content actives 73%, citric acid 20% and titanium dioxide 7%.

Wherein, the Microbe synthesis method of iron content actives, step is as follows:

(1) in reactor, add FeSO4 〃 7H2O and iron protoxide bacillus Acidithtobacillusferrooxidans LX5 suspension thalline, the pH regulating reaction solution with dilute sulphuric acid is 1.5;

(2) mixed solution of step (1) is placed in 28 DEG C of reciprocal shaker shaking culture 1 day;

(3) mixed solution after step (2) being cultivated filters, and the pH of gained throw out sulfuric acid acidation is the distilled water wash 2 times of 1.5, then uses distilled water wash 2 times, dries to obtain product.

Catalyzer described above is for the method for tropeolin-D of degrading, and step is as follows:

(1) get above-mentioned catalyzer 1g to add in the beaker of 1000g containing the waste water of tropeolin-D, fully mix;

(2) under the waste water mixed being placed in room temperature, Keep agitation 2h;

(3) survey its absorbancy at tropeolin-D maximum absorption wavelength 463nm place, in wastewater measurement, the residual content of tropeolin-D, the results are shown in Table 1.

Embodiment 2

For a catalyzer for tropeolin-D in degradation of sewage, its component by weight mark is counted: iron content actives 76%, tartrate 18% and titanium dioxide 6%.

Wherein, the Microbe synthesis method of iron content actives, step is as follows:

(1) in reactor, add FeSO4 〃 7H2O and iron protoxide bacillus Acidithtobacillusferrooxidans LX5 suspension thalline, the pH regulating reaction solution with dilute sulphuric acid is 3.0;

(2) mixed solution of step (1) is placed in 28 DEG C of reciprocal shaker shaking culture 3 days;

(3) mixed solution after step (2) being cultivated filters, and the pH of gained throw out sulfuric acid acidation is the distilled water wash 3 times of 2.5, then uses distilled water wash 3 times, dries to obtain product.

Catalyzer described above is for the method for tropeolin-D of degrading, and step is as follows:

(1) get above-mentioned catalyzer 1g to add in the beaker of 1000g containing the waste water of tropeolin-D, fully mix;

(2) under the waste water mixed being placed in room temperature, Keep agitation 2h;

(3) survey its absorbancy at tropeolin-D maximum absorption wavelength 463nm place, in wastewater measurement, the residual content of tropeolin-D, the results are shown in Table 1.

Embodiment 3

For a catalyzer for tropeolin-D in degradation of sewage, its component by weight mark is counted: iron content actives 76%, oxalic acid 19% and titanium dioxide 5%.

Wherein, the Microbe synthesis method of iron content actives, step is as follows:

(1) in reactor, add FeSO4 〃 7H2O and iron protoxide bacillus Acidithtobacillusferrooxidans LX5 suspension thalline, the pH regulating reaction solution with dilute sulphuric acid is 2.0;

(2) mixed solution of step (1) is placed in 28 DEG C of reciprocal shaker shaking culture 2 days;

(3) mixed solution after step (2) being cultivated filters, and the pH of gained throw out sulfuric acid acidation is the distilled water wash 3 times of 2.0, then uses distilled water wash 3 times, dries to obtain product.

Catalyzer described above is for the method for tropeolin-D of degrading, and step is as follows:

(1) get above-mentioned catalyzer 1g to add in the beaker of 1000g containing the waste water of tropeolin-D, fully mix;

(2) under the waste water mixed being placed in room temperature, Keep agitation 2h;

(3) survey its absorbancy at tropeolin-D maximum absorption wavelength 463nm place, in wastewater measurement, the residual content of tropeolin-D, the results are shown in Table 1.

Table 1

As can be seen from Table 1, iron content actives prepared by the present invention and organic acid, titanium dioxide are in conjunction with the catalyzer of gained, very remarkable for the degradation effect containing tropeolin-D sewage, and action time is short.

The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.

Claims (4)

1. a Microbe synthesis method for iron content actives, it is characterized in that, step is as follows:

(1) in reactor, add FeSO47H2O and iron protoxide bacillus Acidithtobacillusferrooxidans LX5 suspension thalline, the pH regulating reaction solution with dilute sulphuric acid is 1.5 ~ 3.0;

(2) mixed solution of step (1) is placed in 28 DEG C of reciprocal shaker shaking culture 1 ~ 3 day;

(3) mixed solution after step (2) being cultivated filters, and the pH of gained throw out sulfuric acid acidation is the distilled water wash 2 ~ 3 times of 1.5 ~ 2.5, then uses distilled water wash 2 ~ 3 times, dries to obtain product.

2. for a catalyzer for tropeolin-D in degradation of sewage, it is characterized in that, its component by weight mark is counted: iron content actives 73 ~ 76%, organic acid 18 ~ 20% and titanium dioxide 5 ~ 7%.

3. catalyzer according to claim 2, is characterized in that, described organic acid is citric acid, tartrate, oxalic acid or EDTA.

4. catalyzer according to claim 2 is for a method for tropeolin-D of degrading, it is characterized in that, step is as follows:

(1) get catalyzer 1g to add in the beaker of 1000g containing the waste water of tropeolin-D, fully mix;

(2) under the waste water mixed being placed in room temperature, Keep agitation 2h;

(3) its absorbancy is surveyed at tropeolin-D maximum absorption wavelength 463nm place, the residual content of tropeolin-D in wastewater measurement.