CN104958248B - Biological bacteria inhibiting composition and its application in cosmetics - Google Patents
Biological bacteria inhibiting composition and its application in cosmetics Download PDFInfo
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- CN104958248B CN104958248B CN201510395909.6A CN201510395909A CN104958248B CN 104958248 B CN104958248 B CN 104958248B CN 201510395909 A CN201510395909 A CN 201510395909A CN 104958248 B CN104958248 B CN 104958248B
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Abstract
The invention provides a kind of biological bacteria inhibiting composition and its application in cosmetics.The composition includes ε polylysines, nisin, natamycin, glycerine, 1,3 butanediols and pungent glycol, wherein, content ratio of each component in biological bacteria inhibiting composition is：The parts by weight of ε polylysines 0.25~1.0：The parts by weight of nisin 0.1~0.6：The parts by weight of natamycin 0.05~0.4：The parts by weight of glycerine 3~8：The parts by weight of 1,3 butanediol 2~8：The pungent parts by weight of glycol 0.2~2.The composition of the present invention has good bacteriostasis, particularly has significant synergy in terms of Candida albicans, pseudomonas aeruginosa and aspergillus niger is suppressed.The bacteria inhibiting composition of the present invention can be applied in skin type and washing class cosmetics, reduce the addition of original preservative.
Description
Technical field
The present invention is on a kind of biological bacteria inhibiting composition, specifically is used to be added in cosmetics on one kind, has
The biological bacteria inhibiting composition of bacteriostasis, belongs to cosmetic additive agent field.
Background technology
Cosmetics cause corruption during storage and use, easily by the pollution of microorganism.Particularly in cosmetics
Grease, colloid, polyalcohol, protein and the moisture contained, condition is created for the growth of microorganism.In manufacturing process, bag
When dress process, packing material are in itself and consumer uses cosmetics, it is likely to make cosmetics by microorganism pollution.Due to micro-
The effect of biology can cause cosmetics from deterioration, corruption, and its color and luster and smell change on sense organ, so as to lose commodity valency
Value.More importantly the pollution of pathogenic microorganisms can cause the harm of health.Prevent cosmetics due to microorganism pollution and
Harm and product of the generation to human body skin are gone bad, are mouldy, and current maximally effective method is exactly to add preservative.
Traditional Preservative Technology for Personal Care is the growth that can suppress microorganism, a kind of chemical agent bred, such as Buddhist nun
Moor golden ester etc..Nipagin esters are also known as p-hydroxybenzoate, and conventional has methyl hydroxybenzoate, ethyl ester, three kinds of propyl ester, wherein using
It is most be methyl esters.Although methyl hydroxybenzoate preservative is less toxic, usage amount is excessive will to influence health.Therefore, state
There are strict control, China in family to the addition of cosmetics preservative《Hygienic standards for cosmetics》In maximum to nipagin esters permit
Perhaps concentration provides as follows：Mixed ester 10%, single ester 0.4%.
With the progress of scientific research level, industry is also more and more deep to the safety research of preservative, many tradition have by
The preservative used, all it is proven to have certain negative effect.So develop safe pure plant anti-corrosion or biology
Aseptic technic turns into one of R＆D direction of industry.
ε-poly-D-lysine is the microbiology class food preservative for having in current natural antiseptic agent superior antiseptic property.It
It is to be polymerized by 25~30 lysine residues, there is strong bacteriostasis, the fresh-keeping of food can be used for as preservative.
ε-poly-D-lysine antimicrobial spectrum is wide, in acid and slightly acidic environment to gram-positive bacteria, Gram-negative bacteria, saccharomycete,
Mould has certain fungistatic effect, the gram-negative large intestine that ε-poly-D-lysine is not easy to suppress to other natural antiseptic agents
Bacillus, salmonella fungistatic effect are very good, and it also has inhibitory action to heat resistance bacillus and some viruses.ε-more
Polylysine security performance is high, heat endurance is good, water solubility is extremely strong, is widely used in the addition use in food, generally with it is other
Material is used cooperatively, to reach synergy and economic purpose.
Natamycin (Natamycin), it is that a kind of white is made to milky odorless by receiving the controlled fermentation of his streptomycete
Tasteless crystalline powder, generally exists with enol-type structure.Natamycin is a kind of odorless, tasteless, low dosage and safe
Food preservative.Its mechanism of action is combined with the ergosterol of fungi and other sterol groups, checks ergosterol
Biosynthesis, so that cell membrane distorts, seepage is ultimately resulted in, causes cell death.Natamycin both can be effective extensively
Suppress the growth of various moulds, saccharomycete, and can suppresses the generation of mycotoxin, it is fresh-keeping and anti-to can be widely used for food antiseptic
Fungus therapy.Natamycin does not have inhibitory action to bacterium, therefore it does not influence the nature of Yoghourt, cheese, green ham, dry sausage
Maturation.
It is that Buddhist nun is pungent that nisin (Nisin), which is also known as nisin or transliteration, is one caused by streptococcus lactis
Kind peptide material, is made up of, molecular weight is about 3500Da 34 amino acid residues.Because nisin can suppress most of
Gram-positive bacterium, and have strong inhibitory action to the spore of bacillus, therefore extensively should as food preservative
For food service industry.Amino acid is hydrolyzed into quickly under physiological pH condition and the alpha -chymotrypsin effect of human body after edible,
Normal flora in human body intestinal canal will not be changed and produce the resistance problem occurred such as other antibiotic, will not more be resisted with other
There is cross tolerance in rhzomorph, be it is a kind of it is efficient, nontoxic, safely, the natural antiseptic agent that has no side effect.It is existing that oneself is widely used in meat system
Product, dairy products, can, marine product, beverage, fruit drink, liquid egg and egg products, flavouring, wine-making technology, bakery,
In the fields such as instant food, perfume base spices, cosmetics.
Through looking into, do not find to combine epsilon-polylysine, nisin, natamycin into the technology report for cosmetics
Road.
The content of the invention
It is an object of the present invention to provide a kind of biological bacteria inhibiting composition and its correlation at the antibacterial aspect of cosmetics
Using described composition has good fungistatic effect, and is added to small to human health damage in cosmetics.
For the above-mentioned purpose, the invention provides a kind of biological bacteria inhibiting composition, said composition to include epsilon-polylysine, breast
Acid streptococci element, natamycin, glycerine, 1,3-BDO and pungent glycol, wherein, the epsilon-polylysine, nisin,
The content ratio of natamycin, glycerine, 1,3 butylene glycol with pungent glycol in biological bacteria inhibiting composition is：Epsilon-polylysine 0.25
~1.0 parts by weight：The parts by weight of nisin 0.1~0.6：The parts by weight of natamycin 0.05~0.4：The weight of glycerine 3~8
Part：The parts by weight of 1,3 butylene glycol 2~8：The pungent parts by weight of glycol 0.2~2.
According to specific embodiments of the present invention, it is preferable that biological bacteria inhibiting composition of the invention, by epsilon-polylysine,
Nisin, natamycin, glycerine, 1,3 butylene glycol are with pungent glycol according to the parts by weight of epsilon-polylysine 0.25~1.0：Breast
The parts by weight of acid streptococci element 0.1~0.6：The parts by weight of natamycin 0.05~0.4：The parts by weight of glycerine 3~8：1,3 butylene glycol 2~
8 parts by weight：The ratio compounding of the pungent parts by weight of glycol 0.2~2 forms.
According to specific embodiments of the present invention, it is preferable that in biological bacteria inhibiting composition of the invention, the ε-poly- bad ammonia
Acid, nisin, natamycin, glycerine, 1,3 butylene glycol and content ratio of the pungent glycol in biological bacteria inhibiting composition
For：
The parts by weight of epsilon-polylysine 0.25~1.0：The parts by weight of nisin 0.1~0.6：Natamycin 0.05~
0.4 parts by weight：The parts by weight of glycerine 3~5：The parts by weight of 1,3 butylene glycol 3~5：The pungent parts by weight of glycol 1.
Although describing nisin (Nisin) in the prior art can be applied to cosmetic field, it is blue to leather
Escherichia coli, pseudomonas aeruginosa and the saccharomycete of detection are needed in family name's negative bacterium such as cosmetics does not have fungistatic effect；ε-poly
Although lysine has broad-spectrum antibacterial effect, mainly worked in acid and slightly acidic environment, and in the prior art simultaneously
Have no that the technology that ε-poly-D-lysine is used for cosmetics antiseptic and inhibiting bacteria function is reported；Natamycin (Natamycin) does not have to bacterium
Inhibitory action, also have no the prior art report for using it for cosmetics antiseptic and inhibiting bacteria function.Further, since composition is answered in cosmetics
It is miscellaneous, substantial amounts of surfactant and glycerine etc. are usually contained, often the bacteriostasis efficacy of antipathogenic composition is had an impact.
Inventor by substantial amounts of experimental studies have found that, in the present invention by epsilon-polylysine, nisin, receive
The biological bacteria inhibiting composition that his mycin, glycerine, 1,3-BDO and pungent glycol form according to special ratios compounding, each component are mutual
Effect, has good bacteria resistance function, suppresses Escherichia coli, staphylococcus aureus, saccharomycete, breast in applied to cosmetics
Sour bacterium, bacillus subtilis etc. serve certain synergy, suppress white particularly in applied to cosmetics and read
There is significant synergy in terms of pearl bacterium, pseudomonas aeruginosa and aspergillus niger.Wherein, the experiment of inventor shows, ε-poly- bad
Propylhomoserin and glycerine, 1,3 butylene glycol, pungent glycol compound for Candida albicans, aspergillus niger, pseudomonas aeruginosa it is antibacterial
Effect is weaker, and nisin, natamycin respectively with glycerine, 1,3-BDO, pungent glycol compound even without
The obvious effect for suppressing Candida albicans, aspergillus niger, P. aeruginosa growth, and by epsilon-polylysine, streptococcus lactis
Element, natamycin three and glycerine, 1,3 butylene glycol, pungent glycol are then provided with significant suppress in vain after being compounded according to special ratios
Color candida albicans, aspergillus niger, the effect of P. aeruginosa growth.The bacteria inhibiting composition of the present invention can be applied to skin type and washing
In class cosmetics, the addition of original preservative is reduced, while product is had antibacterial and anti-inflammation functions concurrently.So as to which the present invention also carries
Supplied described biological bacteria inhibiting composition in cosmetics are suppressed staphylococcus aureus, Escherichia coli, saccharomycete, lactic acid bacteria,
Bacillus subtilis, pseudomonas aeruginosa, aspergillus niger and/or Candida albicans growth in application, particularly in suppressionization
Application in cosmetic in the growth of Candida albicans, pseudomonas aeruginosa, and/or aspergillus niger.
The biological bacteria inhibiting composition of the present invention, wherein each component will be mixed in proportion.
Present invention also offers described biological bacteria inhibiting composition for preparing the application in cosmetics, specifically it is used
Make preservative to prepare cosmetics, reduce the addition of original preservative.Further, since in the biological bacteria inhibiting composition of the present invention
Glycerine, 1,3-BDO and pungent glycol are included, glycerine, 1,3-BDO and Xin Gan need not can be again added in cosmetic formulations
Alcohol, or can these components be taken the circumstances into consideration with a small amount of addition.
Specifically, the biological bacteria inhibiting composition wherein can account for the weights of cosmetics 0.25%~1.0% by epsilon-polylysine
Amount ratio is added in cosmetics, can play good antibacterial, antisepsis, and will not change the stabilization of former cosmetics substantially
System, preparation-obtained cosmetics still have good stability.
Present invention also offers a kind of cosmetics containing described biological bacteria inhibiting composition.The cosmetics have good
Antibiotic effect.According to specific embodiments of the present invention, in cosmetics of the invention, ε in the biological bacteria inhibiting composition-poly-
Weight content of the lysine in cosmetics is 0.25%~1.0%.Preferably, epsilon-polylysine, nisin in cosmetics
Rhzomorph, the total amount of natamycin are 0.4%~2.0% preferably 0.9%~1.5%.Specifically, biological antibacterial combination of the invention
Thing can apply to skin type or washing class cosmetic product, generally, in skin-care cosmetics such as skin care cream, the life
The addition of thing bacteria inhibiting composition is calculated as 0.4 with the total amount of epsilon-polylysine therein, nisin, natamycin~
1.2g/100g, the addition of original preservative in product can be reduced；It is described in class cosmetics such as hand cleanser products are washed
The addition of biological bacteria inhibiting composition is calculated as 0.9 with the total amount of epsilon-polylysine therein, nisin, natamycin~
1.5g/100g, change original protective system, while product is had suppression antimicrobial and anti-inflammation efficacy concurrently.
In of the invention, added described bacteria inhibiting composition as preservative according to specific embodiments of the present invention
Into the cosmetic formulations of routine (as cosmetic base), you can obtain the cosmetic with good preservative efficacy of the present invention
Product.
In summary, the invention provides one kind to include epsilon-polylysine, nisin, natamycin, glycerine, 1,
The antiseptic composition of 3- butanediols and pungent glycol, said composition have good bacteriostasis, are particularly suppressing white thought
There is synergy in terms of pearl bacterium, pseudomonas aeruginosa and aspergillus niger.The present invention bacteria inhibiting composition can be applied to skin type and
Wash in class cosmetics, reduce the addition of original preservative.
Embodiment
The features of the present invention and possessed technique effect, but this hair is further described below by specific embodiment
It is bright therefore not any way limited.
Embodiment 1, biological bacteria inhibiting composition and bacteriostatic experiment
The present embodiment chooses Escherichia coli, golden yellow using the biological bacteria inhibiting composition of listed each sample in table 1 as test specimen
The functions on common pollutant bacteria such as the pathogenic bacteria such as color staphylococcus, Candida albicans and yeast, lactic acid bacteria, bacillus subtilis, aspergillus niger, is adopted
With paper dish method, bacteriostatic test is targetedly carried out, assesses suppression of each composite biological preservative sample to these common pathogens
Make and use.
Table 1, biological bacteria inhibiting composition formula
1.1 tested strains
Bacterium：Staphylococcus aureus:(Staphylococcus aureus)ATCC 6538
ETEC:(Escherichia coli)ATCC 8739
Pseudomonas aeruginosa:(Pseudomonas.aeruginosa)ATCC 9027
Bacillus subtilis (Bacillus subtilis) ATCC9372
Lactobacillus acidophilus (Lactobacillus acidophilus) ATCC 4356
Mould and saccharomycete：Aspergillus niger (Aspergillus niger) ATCC 16404
Candida albicans (Candida albicans) ATCC 10231
Saccharomyces sake (Saccharomy ces sake) ACCC20045
Note：Above-mentioned bacterial strains provide by Chinese Academy of Sciences American Type Culture Collection committee microorganism fungus kind storehouse.
1.2 for examination culture medium
Mould medium：Czapek's medium, bevel is standby after 115 DEG C of autoclaving 30min.
Bacteria culture media：Lecithin Tween 80-nutrient agar, bevel is standby after 121 DEG C of autoclaving 20min
With.
Yeast culture medium：Czapek's medium, bevel is standby after 115 DEG C of autoclaving 30min.
1.3 test method
1.3.1 experiment prepares indicator bacteria strain being inoculated on test tube slant culture medium, and 37 DEG C of culture 24h, switching is twice
By actication of culture, after actication of culture, fluid nutrient medium is accessed, 37 DEG C of shaken cultivation 24h, prepares bacteria suspension；
Each bacteria inhibiting composition test specimen in table 1 is weighed, the formula solution of 15% concentration is configured to deionized water；
Diameter 0.6cm circular filter paper piece is made of card punch.
1.3.2 the bacteriostatic test of each bacteria inhibiting composition uses paper dish method, with Escherichia coli, staphylococcus aureus, white
Candida albicans, bacillus subtilis, black aspergillus, pseudomonas aeruginosa, saccharomycete (saccharomyces sake) and lactic acid bacteria (lactobacillus acidophilus)
Respectively indicator bacteria, 0.1mL indicator bacterias bacteria suspension is taken respectively in culture medium flat plate, placement immersion uniform with sterilizing spatula coating
The presence or absence of the circular filter paper piece of 1 each test sample solution of table of over-richness 15%, 37 DEG C of incubated 24h, observation inhibition zone, just
Step assesses fungistatic effect.
1.4 result and analysis
Result of the test is listed in table 2.
Antibacterial circle diameter/cm of table 2, each preservative
Note："-" is represented around circular filter paper piece without obvious inhibition zone (antibacterial circle diameter can not accurate and effective measurement).
As shown in Table 2, sample 10 to gram-positive bacterium staphylococcus aureus, bacillus subtilis, lactic acid bacteria with
Gramnegative bacterium Escherichia coli, pseudomonas aeruginosa and fungi Candida albicans, saccharomycete, aspergillus niger and lactic acid bacteria have
Certain fungistatic effect, wherein to Escherichia coli, saccharomycete, bacillus subtilis, aspergillus niger, pseudomonas aeruginosa antibacterial effect
Fruit is weaker；Sample 11 can effectively suppress gram-positive bacterium such as staphylococcus aureus, lactic acid bacteria, bacillus subtilis
Growth, but do not have to gramnegative bacterium such as Escherichia coli, P. aeruginosa and saccharomycete, Candida albicans, aspergillus niger etc.
Obvious fungistatic effect；Sample 12 can effectively suppress the growth of gramnegative bacterium Escherichia coli and saccharomycete, but to other
Bacterium does not have obvious inhibition.And 1~9 pair of each bacterium of sample is respectively provided with significant fungistatic effect, particularly suppressing Candida albicans
Bacterium, aspergillus niger, pseudomonas aeruginosa growth in terms of, serve synergy between each component in sample 1~9.
The emulsified make-up (cold cream or face cream etc.) of biological bacteria inhibiting composition of the application example 1 containing the present invention,
Formula is：
Biological bacteria inhibiting composition (sample 5 is formulated in table 1)：With epsilon-polylysine therein, nisin, receive that he is mould
The total amount meter of element, 0.5%
Olive oil：4%
Vitwas E：1.5%
Hazel extract：3%
Laruyl alcohol sodium sulfovinate：0.8%
Ceteth -20：1%
Nexbase 2004：8%
Dimethyl silicone polymer：6%
Isopropyl palmitate：8%
Excipient thickener：6%
Glycerine：2%
1,3 butanediols：1%
Nutrition, functional components：Optionally, formula ratio
Preservative：IPBC- I (0.005%), it is the 50% of convention amount
Bronopol (0.01%), it is the 50% of convention amount
Deionized water：Surplus.
The method for preparing the cosmetics, the raw material composition of the cosmetics include oil phase, aqueous phase and of the present invention
Biological bacteria inhibiting composition, this method include：
First make oil phase and the aqueous phase progress of cosmetic material primary emulsifying, adjust the temperature of the emulsification system, be cooled to 35
~40 DEG C, add olive oil, hamamelis extract solution, biological bacteria inhibiting composition (as be formulated in have other addition mutually as nutrition,
Functional components, other addition phases are added in this step), system is agitated uniformly, further according to recipe requirements after cooling
Complete operation.
The skin care cream that the present embodiment obtains, fragrant, pleasant aroma, without unacceptable peculiar smell.By the resistance to of 24h
Cold (5 ± 1 DEG C) heat-resisting (40 ± 1 DEG C) placements test, system is normal, and nodeless mesh separates out, no muddy, discoloration, lamination.Through length
Futures frame storage test and repeatedly heat-resisting, cold-resistant destructive testing, product items sense index, physical and chemical index, sanitary index are equal
Meet corresponding national standard.
The washing class product (hand cleanser etc.) of biological bacteria inhibiting composition of the application example 2 containing the present invention, formula is：
Biological bacteria inhibiting composition (sample 8 is formulated in table 1)：With epsilon-polylysine therein, nisin, receive that he is mould
The total amount meter of element, 1.0%
Laureth sodium sulfovinate：5%
Cocounut oil acyl disodium glutamate：5%
NMF：6%
Cocoyl glucoside glyceryl oleate：1%
Excipient thickener：0.1%
Preservative：DMDMH：0.1%, it is 1/3rd of convention amount
Deionized water：Surplus.
The method for preparing the cosmetics, the raw material composition of the cosmetics include surfactant, skin care ingredient and sheet
The described biological bacteria inhibiting composition of invention, this method include：
The surfactant and excipient thickener for first making cosmetic material are completely dissolved in deionized water, adjust the system
Temperature, be cooled to 35~40 DEG C, add moisturizing ingredient and biological bacteria inhibiting composition, system is agitated uniformly, further cooling
Complete to operate according to recipe requirements afterwards.
The hand cleanser that the present embodiment obtains, smell is pleasant, without unacceptable peculiar smell.By 24h cold-resistant (5 ± 1 DEG C)
Heat-resisting (40 ± 1 DEG C) are placed test, and system is normal, and nodeless mesh separates out, no muddy, discoloration, lamination.Through long-term shelf storage
Experiment and repeatedly heat-resisting, cold-resistant destructive testing, product items sense index, physical and chemical index, sanitary index meet accordingly
National standard.
The antibacterial challenge experiment of preservative
Specimen material and culture medium
Mould medium：Czapek's medium, bevel is standby after 115 DEG C of autoclaving 30min.
Bacteria culture media：Lecithin Tween 80-nutrient agar, bevel after 121 DEG C of autoclaving 20min
It is standby.
Test strain：
Bacterium：Staphylococcus aureus:(Staphylococcus aureus)ATCC 6538
ETEC:(Escherichia coli)ATCC 8739
Pseudomonas aeruginosa:(Pseudomonas.aeruginosa)ATCC 9027
Bacillus subtilis (Bacillus subtilis) ATCC9372
Lactobacillus acidophilus (Lactobacillus acidophilus) ATCC 4356
Mould and saccharomycete：Aspergillus niger (Aspergillus niger) ATCC 16404
Candida albicans (Candida albicans) ATCC 10231
Saccharomyces sake (Saccharomy ces sake) ACCC20045
Note：Above-mentioned bacterial strains provide by Chinese Academy of Sciences American Type Culture Collection committee microorganism fungus kind storehouse.
The preparation of experiment bacterium solution：
Each bacterial strain is inoculated into corresponding medium slant, bacterium (staphylococcus aureus, large intestine angstrom respectively before experiment
Uncommon Salmonella, pseudomonas aeruginosa, bacillus subtilis, lactobacillus acidophilus) 36~48 are cultivated in 36 ± 1 DEG C of constant incubators
Hour.The appropriate bacterium colony of picking mixes in sterile saline respectively, and certain density plastc ring is made.Mixed cell is hanged
Liquid total concentration is about 1.0 × 108cfu/ml.It is standby to be placed in 4 DEG C of storages.It is small that mould cultivates 120 in 27 ± 1 DEG C of constant incubators
When.Saccharomycete is cultivated 24 hours in 27 ± 1 DEG C of constant incubators, and the appropriate yeast and mold bacterium colony of picking is in sterile physiological salt
It is well mixed in water, certain density plastc ring is made.Mixing yeast and mold spore suspension total concentration is about 1.0 ×
107cfu/ml.It is standby to be placed in 4 DEG C of storages.
Test method：
Using once adding 28 days Microbial Challenges of bacterium to test, this method is with reference to Microbial Challenge examination on American Pharmacopeia
The method for testing detection preservative effectiveness.The washing class production of the above-mentioned emulsified make-up of application example 1 and application example 2 is weighed respectively
Product 100g loads in appropriate plastic bottle as test sample, is separately added into appropriate plastc ring, every gram of sample is finally contained
Amount of bacteria is 1.0 × 106~5.0 × 106CFU/g, mould yeast bacterium amount are 1.0 × 105~5.0 × 105CFU/g is fully mixed,
It is respectively placed in 36 ± 1 DEG C of constant incubators and cultivates.In inoculation the 0th day, the 3rd day, the 7th day, the 14th day, the 21st day and the 28th day
Sampling carries out bacterium amount analysis.
Evaluation criterion：
Initial inoculum bacterium 106Cfu/g~107Cfu/g (mL), mould 105Cfu/g~106cfu/g(mL)]：1. the
At 28 days, bacterium or mould are contained in sample>103Cfu/g (mL), the sample can not be tested by the challenge of microorganism attack, table
The protective system of bright sample can not effectively act as suppressing the effect of microorganism, and product is easy in production, storage and in
Polluted by microorganism.2. at the 28th day, containing bacterium or mould 10 in sample2Cfu/g~103Cfu/g (mL), the sample
Conditionally tested by challenging, i.e., when protein in product or other animals and plants material compositions are not especially high, produced simultaneously
Hygienic conditions meet the requirements, when packing material is not susceptible to secondary pollution, the mould proof system can use, and otherwise can not.3. the
At 28 days, containing bacterium or mould in 10cfu/g~10 in sample2Cfu/g (mL), show the protective system of the sample to microorganism
There is stronger suppression to kill effect, by challenge test, product production, storage and using when be not readily susceptible to microorganism pollution.④
From the 7th day, bacterium or mould in sample<10cfu/g (mL), illustrate the protective system of the sample has extra-heavy to microorganism
Inhibitory or killing effect, by challenge test, product production, storage and using when be not easy very much microbial contamination.
Preservative system
1# is the sample of above-mentioned application example 1, and its protective system is biological bacteria inhibiting composition, IPBC- I and Bronopol.
2#, its protective system is iodine propilolic alcohol butyl mephenesin Carbamate and bromo- 2- nitropropanes -1, the 3- glycol of 2-, dosage are
Convention amount, be not added with the present invention biological bacteria inhibiting composition, add glycerine 8% and 1,3 butanediols 6%, remaining recipe ingredient with
The sample of application example 1 is identical.
3# is the sample of application example 2, and its protective system is biological bacteria inhibiting composition and DMDMH.
4#, its protective system are DMDMH and methylisothiazolinone, and dosage is convention amount, is not added with the present invention
Biological bacteria inhibiting composition, add glycerine 3% and 1,3 butanediols 5%, remaining recipe ingredient is identical with the sample of application example 2.
Specific combination is shown in Table 3.
Protective system used in table 3
* the numeral in table is the percentage that each material composition accounts for cosmetics weight
As a result with discussion
Microbial Challenge result of the test see the table below 4.
The Microbial Challenge result of the test of table 4
The Microbial Challenge result of the test carried out to the sample of 4 protective systems shows：
At (1) the 28th day, bacterium, the content of yeast and mold are equal in each sample<10cfu/g (mL), show this 4 kinds
Protective system has stronger suppression to kill effect microorganism, by challenge test, product production, storage and using when be not easy
By microorganism pollution.
(2) 1# compares with 2#, and at the 7th day, total number of bacterial colonies was lower, and the ability for suppressing mould saccharomycete is bright after 7 days
It is aobvious to embody.
(3) 3# compares with 4#, and at the 7th day, total number of bacterial colonies was lower, and the ability for suppressing mould saccharomycete is bright after 7 days
It is aobvious to embody.
Conclusion
Testing proves that 1#, 2#, 3#, 4# protective system are tested by challenging, and 1# and 2# is skin care cream sample, and 1# is selected
With biological bacteria inhibiting composition and the protective system of the chemical preservative collocation for conventional addition half, 2# is added from conventional
The chemical preservation system of dosage, according to result of the test, 1# fungistatic effects are suitable with 2#, and the detection data later from the 7th day are demonstrate,proved
Bright, its inhibition to bacterium is better.
The protective system of chemical preservative collocation of the 3# from biological bacteria inhibiting composition and for conventional addition 1/3rd,
4# selects the chemical preservation system of conventional addition, and according to result of the test, 3# fungistatic effects are suitable with 4#, and after the 7th day
Testing number it was demonstrated that its fungistatic effect to bacterium is better.
Claims (13)
- A kind of 1. life of biological bacteria inhibiting composition Candida albicans, pseudomonas aeruginosa and/or aspergillus niger in cosmetics are suppressed Application in length, wherein, the biological bacteria inhibiting composition include epsilon-polylysine, nisin, natamycin, glycerine, 1,3-BDO and pungent glycol, wherein, the epsilon-polylysine, nisin, natamycin, glycerine, 1,3-BDO It is with content ratio of the pungent glycol in biological bacteria inhibiting composition：The parts by weight of epsilon-polylysine 0.25~1.0：Nisin 0.1~0.6 parts by weight：The parts by weight of natamycin 0.05~0.4：The parts by weight of glycerine 3~8：The parts by weight of 1,3 butylene glycol 2~8：It is pungent The parts by weight of glycol 0.2~2.
- 2. application according to claim 1, wherein, the biological bacteria inhibiting composition is by epsilon-polylysine, streptococcus lactis Element, natamycin, glycerine, 1,3 butylene glycol are with pungent glycol according to the parts by weight of epsilon-polylysine 0.25~1.0：Nisin 0.1~0.6 parts by weight：The parts by weight of natamycin 0.05~0.4：The parts by weight of glycerine 3~8：The parts by weight of 1,3 butylene glycol 2~8：It is pungent The ratio compounding of the parts by weight of glycol 0.2~2 forms.
- 3. application according to claim 1 or 2, wherein, it is the epsilon-polylysine, nisin, natamycin, sweet Oil, content ratio of the 1,3 butylene glycol with pungent glycol in biological bacteria inhibiting composition are：The weight of epsilon-polylysine 0.25~1.0 Part：The parts by weight of nisin 0.1~0.6：The parts by weight of natamycin 0.05~0.4：The parts by weight of glycerine 3~5：1,3- fourths two The parts by weight of alcohol 3~5：The pungent parts by weight of glycol 1.
- 4. a kind of application of biological bacteria inhibiting composition in cosmetics are prepared, wherein, the biological bacteria inhibiting composition includes ε-poly- Lysine, nisin, natamycin, glycerine, 1,3-BDO and pungent glycol, wherein, the epsilon-polylysine, lactic acid The content ratio of streptostacin, natamycin, glycerine, 1,3 butylene glycol with pungent glycol in biological bacteria inhibiting composition is：ε-poly- The parts by weight of lysine 0.25~1.0：The parts by weight of nisin 0.1~0.6：The parts by weight of natamycin 0.05~0.4：Glycerine 3~8 parts by weight：The parts by weight of 1,3 butylene glycol 2~8：The pungent parts by weight of glycol 0.2~2.
- 5. application according to claim 4, wherein, the biological bacteria inhibiting composition is by epsilon-polylysine, streptococcus lactis Element, natamycin, glycerine, 1,3 butylene glycol are with pungent glycol according to the parts by weight of epsilon-polylysine 0.25~1.0：Nisin 0.1~0.6 parts by weight：The parts by weight of natamycin 0.05~0.4：The parts by weight of glycerine 3~8：The parts by weight of 1,3 butylene glycol 2~8：It is pungent The ratio compounding of the parts by weight of glycol 0.2~2 forms.
- 6. the application according to claim 4 or 5, wherein, it is the epsilon-polylysine, nisin, natamycin, sweet Oil, content ratio of the 1,3 butylene glycol with pungent glycol in biological bacteria inhibiting composition are：The weight of epsilon-polylysine 0.25~1.0 Part：The parts by weight of nisin 0.1~0.6：The parts by weight of natamycin 0.05~0.4：The parts by weight of glycerine 3~5：1,3- fourths two The parts by weight of alcohol 3~5：The pungent parts by weight of glycol 1.
- 7. according to the application described in claim 1 or 2 or 4 or 5, wherein, the biological bacteria inhibiting composition is relied with wherein ε-poly- The part by weight that propylhomoserin accounts for cosmetics 0.25%~1.0% is added in cosmetics.
- 8. according to the application described in claim 1 or 2 or 4 or 5, wherein, the cosmetics are skin type or washing class cosmetics.
- 9. a kind of cosmetics, wherein containing a kind of biological bacteria inhibiting composition, the biological bacteria inhibiting composition include epsilon-polylysine, Nisin, natamycin, glycerine, 1,3-BDO and pungent glycol, wherein, the epsilon-polylysine, streptococcus lactis The content ratio of element, natamycin, glycerine, 1,3 butylene glycol with pungent glycol in biological bacteria inhibiting composition is：Epsilon-polylysine 0.25~1.0 parts by weight：The parts by weight of nisin 0.1~0.6：The parts by weight of natamycin 0.05~0.4：The weight of glycerine 3~8 Measure part：The parts by weight of 1,3 butylene glycol 2~8：The pungent parts by weight of glycol 0.2~2.
- 10. cosmetics according to claim 9, wherein, the biological bacteria inhibiting composition is by epsilon-polylysine, nisin Rhzomorph, natamycin, glycerine, 1,3 butylene glycol are with pungent glycol according to the parts by weight of epsilon-polylysine 0.25~1.0：Streptococcus lactis Plain 0.1~0.6 parts by weight：The parts by weight of natamycin 0.05~0.4：The parts by weight of glycerine 3~8：The parts by weight of 1,3 butylene glycol 2~8： The ratio compounding of the pungent parts by weight of glycol 0.2~2 forms.
- 11. the cosmetics according to claim 9 or 10, wherein, the epsilon-polylysine, nisin, receive that he is mould The content ratio of element, glycerine, 1,3 butylene glycol with pungent glycol in biological bacteria inhibiting composition is：Epsilon-polylysine 0.25~1.0 Parts by weight：The parts by weight of nisin 0.1~0.6：The parts by weight of natamycin 0.05~0.4：The parts by weight of glycerine 3~5：1,3- The parts by weight of butanediol 3~5：The pungent parts by weight of glycol 1.
- 12. the cosmetics according to claim 9 or 10, wherein, the epsilon-polylysine in the biological bacteria inhibiting composition exists Weight content in cosmetics is 0.25%~1.0%.
- 13. the cosmetics according to claim 9 or 10, the cosmetics are skin type or washing class cosmetics.
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| CN106265120B (en) * | 2016-09-12 | 2019-04-16 | 珠海金肽生物科技有限公司 | A kind of cytokine-like effect and cosmetic applications of nine Oligopeptide Compositions |
| CN108210392A (en) * | 2018-03-23 | 2018-06-29 | 长沙小新新能源科技有限公司 | A kind of lasting disinfection hand cleanser and preparation method thereof |
| CN108324586A (en) * | 2018-04-08 | 2018-07-27 | 北京皓尔生物科技有限公司 | A kind of antibacterial anti-inflammatory composition and its application |
| CN109294522B (en) * | 2018-11-09 | 2021-06-22 | 山东省药学科学院 | Bacteriostatic indoor dust suppressant |
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| CN110393674A (en) * | 2019-08-22 | 2019-11-01 | 南京华狮新材料有限公司 | Amino acid antibacterial agent synergy hexamidine bactericidal composition |
| CN111019748A (en) * | 2019-11-25 | 2020-04-17 | 清华大学天津高端装备研究院 | Biological stabilizer for inhibiting metal processing liquid from putrefaction and preparation method and application thereof |
| CN110960445A (en) * | 2019-12-30 | 2020-04-07 | 福建恒安集团有限公司 | Anticorrosion system for baby wet tissue immersion liquid and preparation method thereof |
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