CN104957190A - Sterilizing agent for livestock breeding as well as preparation method and application thereof - Google Patents
Sterilizing agent for livestock breeding as well as preparation method and application thereof Download PDFInfo
- Publication number
- CN104957190A CN104957190A CN201510376958.5A CN201510376958A CN104957190A CN 104957190 A CN104957190 A CN 104957190A CN 201510376958 A CN201510376958 A CN 201510376958A CN 104957190 A CN104957190 A CN 104957190A
- Authority
- CN
- China
- Prior art keywords
- parts
- bactericide
- korean pine
- preparation
- peptide
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to the field of animal hygiene, and particularly relates to a sterilizing agent for livestock breeding. The sterilizing agent comprises the following raw materials in parts by weight: 10 to 15 parts of spirulina peptide, 6 to 8 parts of red raspberry extract, 6 to 8 parts of pinus koraiensis polyphenol, 3 to 5 parts of alpha-spiral antibacterial peptide, 1 to 3 parts of polyhexamethyleneguanidine and 61 to 74 parts of deionized water. The sterilizing agent is used for livestock breeding. Compared with the prior art, the sterilizing agent has the following advantages: (1) marine active thromboplastin and plant extract are used as sterilizing components, so that colibacillus, staphylococcus aureus and candida albicans can be effectively killed, the sterilizing effect is good, and the sterilizing spectrum is wide; (2) the function is stable not susceptible to the influence of temperature, organic matters and acidity and alkality, capable of penetrating the organic matters and cracks and capable of rapidly killing the bacteria and virus; (3) the sterilizing agent is harmless and non-irritable to the human beings, animals and poultry, and the drug has no corrosion after being diluted by water.
Description
Technical field
The present invention relates to field of animal health, be specifically related to a kind of livestock-raising bactericide.
Background technology
The large-scale intensive Main way having become China's animal husbandry development, and antibiotic medicine all plays an important role in control animal bacteria disease and promotion animal growth, the quantity of livestock and poultry and the output of animal products are greatly improved, obtain comparatively considerable economic benefit.Therefore, feeding antibiotic uses extensively at livestock.Which results in CR Critical feed safety hidden danger.In these years, the food safety affair again and again occurred causes just because of feed safety problem, and especially the use of the long-term sub-doses of feeding antibiotic is to become one of currently known major reason.In addition, European Union's in March, 2002 aspect claims to detect chloramphenicol and monoconal antibody mediated furacilinum residue from some aquatic products of Chinese import, starts the animal derived food comprehensively stopping import China; The measure that August, Switzerland specified mandatory detection Nitrofuran metabolites because finding to have antibiotic residue from the poultry of Chinese import, etc.The result of above-mentioned event causes overstocking of a large amount of animal derived food, and over one hundred enterprise stops production, and economic loss is great.China is as Production of Livestock and Poultry big country, and due to medicament residue, particularly antibiotic residue such as to exceed standard at the problem, and export volume only accounts for the 0.9%-l.2% of output.
From last century the seventies, in order to limit the abuse of feeding antibiotic, countries in the world and area are proposed various relevant decree regulation one after another.1986, Sweden became first permission and add antibiotic country in feed; 2006, European Union completely forbade the use of antibiotics as feed addictive in aquaculture; 2005, in order to clearly to the strict forbidding of Multiple Classes of Antibiotics class medicine, the Ministry of Agriculture of China issued " No. 560 bulletins ".
Therefore, the nuisanceless green additive finding energy substitute antibiotics class just becomes the task of top priority of the nuisanceless livestock products of China.
Summary of the invention
The technical problem that the present invention solves is: feeding antibiotic is widely used as bactericide to use in livestock products, but brings counter productive simultaneously, is exactly that feed exists potential safety hazard.
Specifically, for the deficiencies in the prior art, the invention provides following technical scheme:
The invention provides a kind of livestock-raising bactericide, it is nuisanceless green additive, and raw material composition and the parts by weight thereof of making this bactericide are: spirulina peptide 10-15 part, raspberry extract 6-8 part, Korean pine polyphenol 6-8 part, alpha-helix antibacterial peptide 3-5 part, polyhexamethylene guanide 1-3 part, deionized water 63-69 part.
Preferably, raw material composition and the parts by weight thereof of making this bactericide are: spirulina peptide 12 parts, raspberry extract 7 parts, Korean pine polyphenol 7 parts, alpha-helix antibacterial peptide 4 parts, polyhexamethylene guanide 2 parts, deionized water 68 parts.
Wherein spirulina peptide has certain inhibitory action to staphylococcus aureus, Escherichia coli, bacillus subtilis, and harmless to human non-toxic, so spirulina peptide is being with a wide range of applications at food, medicine, health field as natural antiseptic agent, bactericide; Very strong killing action is had to the common pathogenic microorganism such as livestock and poultry bacterium, brood cell, virus.Meanwhile, be again the good disinfectant in livestock and poultry place, be applicable to various veterinary disinfection, be particularly useful for the regular disinfection of drinking water of fowl, to poultry, fowl itself sterilization, plant egg sterilization and animal product process, food processing time environment, apparatus and fresh fruit-vegetable food sterilization.
In raspberry extract, ellagic acid composition has good reducing power, effectively can remove hydroxyl radical free radical and DPPH free radical, and have good inhibitory action to test bacteria such as Escherichia coli, salmonella, hay bacilluses;
The hydrophobic bond of Korean pine polyphenol and multidigit point hydrogen bond can with the carbonyl generation specific bindings on protein, and Korean pine polyphenol also as alkaloid, polysaccharide etc., molecule recombination reaction can occur with the large molecule of other biological, shows as the characteristic of these compounds; Korean pine polyphenol contains a large amount of neighbour two phenolic hydroxyl groups, these adjacent two phenolic hydroxyl groups can with some metal ion generation complex reaction, in biologically active, show multiple using value.Containing a large amount of Korean pine polyphenol in the extract of pinaceae plant (as Korean pine), it has antibacterial and antiviral activity.
The amphipathic molecule of cationic (containing too much lysine and arginine) that alpha-helix antibacterial peptide is made up of 12 ~ 50 amino acid residues.There is heat endurance, under larger ion strength and lower or higher pH value, still can keep stronger activity; Alpha-helix antibacterial peptide both can have been killed or suppress gram-positive bacteria, can kill again or suppress Gram-negative bacteria.
Polyhexamethylene guanide is the high molecular polymer of long-chain, guanidine radicals has very high activity, polymer is made to become electropositive, usually in electronegative each bacterioid, virus is easy to be adsorbed, then firmly bacterium can be wound around, thus inhibit the splitting function of bacterium and virus, make bacterium, virus loses fecundity, add that polymer molecule plugs the respiration channel of microorganism, microorganism is choked to death rapidly; In addition, due to the high molecular polymer long chain type structure of polyhexamethylene guanide uniqueness, not containing heterocyclic group, not easily absorbed by animal body inner tissue, so there is no toxicity to zooblast, thus can accomplish human non-toxic.
The present invention using ocean effective bios, plant extracts as sterilization component, integrate the new and effective poultry decontaminant that washing, sterilization, sterilizing etc. are multi-functional, be applicable to the regular sterilization of the environment of livestock, tableware, vessel and drinking-water, the particularly sterilization of epidemic situation phase.Effectively can kill Escherichia coli, staphylococcus aureus and white and read coccus.
The present invention also provides the preparation method of above-mentioned bactericide, comprises the steps:
(1) spirulina peptide, raspberry extract, Korean pine polyphenol are carried out composite in proportion, emulsification 20min;
(2) alpha-helix antibacterial peptide, polyhexamethylene guanide, deionized water are added in step (1) in proportion, stir;
(3) after the assay was approved, packing, labeling, sealing.
Preferably, the preparation of described raspberry extract:
Red raspberry fruit is carried out making beating process;
Refluxing extraction, vacuum filtration again, Effect of Alcohol Extracting Procedureto Total: reflux temperature 80 DEG C, return time 90min, vacuum filtration technique: the centrifugal 10min of 4000rpm rotating speed, gets supernatant;
Described supernatant rotary evaporation in vacuo is obtained concentrate, by concentrate vacuum cooling drying, obtains raspberry extract.
Preferably, the preparation of described Korean pine polyphenol:
Korean pine is dried, and impurity elimination, pulverizes, sieves;
Ultrasonic extraction, vacuum filtration again, ultrasonic extraction process: ultrasonic temperature 50 DEG C, ultrasonic time 4min, vacuum filtration technique: the centrifugal 10min of 4000rpm rotating speed, gets supernatant;
Described supernatant rotary evaporation in vacuo is obtained concentrate, by concentrate vacuum cooling drying, obtains Korean pine polyphenol.
By bactericide prepared by the inventive method, Be very effective, its reason is to obtain raspberry extract by spirulina peptide, red raspberry fruit, Korean pine obtains Korean pine polyphenol system, active component in each component of effective extraction, thus reach the effect of the drug effect better playing each component, by test of many times, effectively can kill Escherichia coli, staphylococcus aureus and white and read coccus, have no side effect again.
Bactericide of the present invention is used for livestock-raising, and compared with prior art, tool has the following advantages:
1) using ocean, effective cytokines, plant extracts are as sterilization component, and effectively can kill Escherichia coli, staphylococcus aureus and white and read coccus, bactericidal effect is good, fungicidal spectrum is wide;
2) stable effect, does not affect by temperature, organic matter, acid-base value, can penetrate organic matter and crack, quick kill bacteria and virus;
3) to people, animal and poultry harmless non-stimulated, medicament through water dilution after non-corrosiveness;
4) easy to use, available various spraying apparatus is prepared at the scene and sprays, and drug effect is significantly reliable;
5) abundant raw material, simple process, low price, easy for operation.
Embodiment
Bactericide involved in the present invention comprises a kind of spirulina peptide, a kind of raspberry extract, a kind of Korean pine polyphenol, a kind of alpha-helix antibacterial peptide, a kind of polyhexamethylene guanide.
As one embodiment of the present of invention, spirulina peptide obtains by directly buying.Preferably, the preparation of spirulina peptide: can multigelation be adopted to prepare spirulina protein in conjunction with ultrasonic fragmentation spirulina, utilizes sephadex column to carry out separation and purification and obtains spirulina peptide.The present invention adopt spirulina peptide and other natural cytokines composite, realize novel quick-acting bactericidal effect, its toxicity is low, noresidue, to person poultry harmless, good to animal safety, can tackle hard water and grease, penetrating odor is not dense, good stability.
As one embodiment of the present of invention, raspberry extract is obtained by the making beating of red raspberry fruit, refluxing extraction, suction filtration, rotary evaporation, freeze drying; By refluxing extraction, obtain effective ingredient; Raspberry extract has good oxidation resistance, can develop as a kind of new plant-source antibacterial composition and utilize.
As one embodiment of the present of invention, Korean pine polyphenol by oven dry, the impurity elimination of Korean pine, pulverize, sieve, ultrasonic extraction, suction filtration, rotary evaporation, freeze drying obtain; By ultrasonic extraction, obtain effective ingredient; Korean pine polyphenol can suppress the growth of helicobacter pylori, and median lethal concentration is 12.5g/mL.
As one embodiment of the present of invention, alpha-helix antibacterial peptide and polyhexamethylene guanide are commercially available obtaining.Wherein the antimicrobial spectrum of marine organisms alpha-helix antibacterial peptide is wide compared with conventional antibiotic, conventional antibiotic is usually only effective to bacterium, and from the existing anti-gram positive bacteria of marine organisms alpha-helix antibacterial peptide of insect, pig, the frog, people etc. and Gram-negative bacteria effect, have again antimycotic, antivirus action; The wherein physical sterilization mechanism of polyhexamethylene guanide, makes each bacterioid, virus not develop immunity to drugs to it by DNA variation, thus reach fast, wide spectrum, stable effect killing all kinds of bacterial virus
Marine organisms spirulina peptide of the present invention and alpha-helix antibacterial peptide, and the composite collaborative use of plant extracts raspberry extract and Korean pine polyphenol, be considered to killing Escherichia coli, staphylococcus aureus and white that to read coccus etc. be favourable.
Bactericide of the present invention and preparation method thereof is further illustrated below by specific embodiment.
Embodiment one
Prepare the bactericide as defined composition in table 1, preparation detailed process is as follows:
The preparation of raspberry extract: red raspberry fruit is carried out making beating process, then refluxing extraction, vacuum filtration, obtain concentrate by filter vacuum rotary evaporation, by concentrate vacuum cooling drying, obtain raspberry extract, for subsequent use; Wherein Effect of Alcohol Extracting Procedureto Total: reflux temperature 80 DEG C, return time 90min, red raspberry fruit: 85% acetone solid-liquid ratio is 1:12 (solid-liquid ratio unit g/mL); Wherein vacuum filtration technique: the centrifugal 10min of 4000rpm rotating speed, gets supernatant; Wherein red raspberry fruit is commercially available;
The preparation of Korean pine polyphenol: Korean pine dry, impurity elimination, pulverizes, sieves, more ultrasonic extraction, vacuum filtration, filter vacuum rotary evaporation is obtained concentrate, by concentrate vacuum cooling drying, obtains Korean pine polyphenol, for subsequent use; Wherein Korean pine is dried, and is specially low temperature drying Korean pine to constant weight; Wherein pulverize, sieve, being specially high speed disintegrator and pulverizing, sieves in 20 order apertures; Wherein ultrasonic extraction process: ultrasonic temperature 50 DEG C, ultrasonic time 4min, Korean pine: 50% methyl alcohol solid-liquid ratio is 1:40 (solid-liquid ratio unit g/mL); Wherein vacuum filtration technique: the centrifugal 10min of 4000rpm rotating speed, gets supernatant; Wherein Korean pine is commercially available;
Spirulina peptide, raspberry extract, Korean pine polyphenol are carried out composite in ratio described in table 1, emulsification 20min;
Alpha-helix antibacterial peptide, polyhexamethylene guanide are added in the intermediate after above-mentioned emulsification, and adds water and stir;
Sample immediately, after the assay was approved, packing, labeling, sealing.
This bactericide is referred to as sample 1, sample 2, sample 3, sample 4 here.
Each raw material addition (unit: kg) in table 1 embodiment one
The degermation of bactericide of the present invention is relevant with the proportioning of each raw material, develops well, brings wide exploitation prospect can to the field such as clinical medicine, clinical pharmacy, food processing, animal and plant transgenic technology.
Bactericide stable effect of the present invention, does not affect by temperature, organic matter, acid-base value; With the method for electric charge and bacterial cell membrane interaction during marine organisms alpha-helix antibacterial peptide passes through, organic matter and crack can be penetrated, quick kill bacteria and virus, considerably reduce the possibility that bacterium produces drug resistance.
Medicament of the present invention is non-corrosiveness after water dilution, and easy to use, available various spraying apparatus is prepared at the scene and sprays, and drug effect is significantly reliable.
The present invention can summarize with other the concrete form without prejudice to spirit of the present invention or principal character.Therefore, no matter from which point, above-mentioned embodiment of the present invention all can only be thought explanation of the present invention and can not limit the present invention, claims indicate scope of the present invention, and scope of the present invention is not pointed out in above-mentioned explanation, therefore, any change in the implication suitable with claims of the present invention and scope, all should think in the scope of the claims being included in health care pedicure device of the present invention.
Claims (5)
1. a livestock-raising bactericide, it is characterized in that, raw material composition and the parts by weight thereof of making this bactericide are: spirulina peptide 10-15 part, raspberry extract 6-8 part, Korean pine polyphenol 6-8 part, alpha-helix antibacterial peptide 3-5 part, polyhexamethylene guanide 1-3 part, deionized water 61-74 part.
2. bactericide according to claim 1, it is characterized in that, raw material composition and the parts by weight thereof of making this bactericide are: spirulina peptide 12 parts, raspberry extract 7 parts, Korean pine polyphenol 7 parts, alpha-helix antibacterial peptide 4 parts, polyhexamethylene guanide 2 parts, deionized water 68 parts.
3. the preparation method of bactericide described in claim 1 or 2, comprises the steps:
(1) spirulina peptide, raspberry extract, Korean pine polyphenol are carried out composite in proportion, emulsification 20min;
(2) alpha-helix antibacterial peptide, polyhexamethylene guanide, deionized water are added in step (1) in proportion, stir;
(3) after the assay was approved, packing, labeling, sealing.
4. the preparation method of bactericide described in claim 3, is characterized in that, the preparation of described raspberry extract:
Red raspberry fruit is carried out making beating process;
Refluxing extraction, vacuum filtration again, Effect of Alcohol Extracting Procedureto Total: reflux temperature 80 DEG C, return time 90min, vacuum filtration technique: the centrifugal 10min of 4000rpm rotating speed, gets supernatant;
Described supernatant rotary evaporation in vacuo is obtained concentrate, by concentrate vacuum cooling drying, obtains raspberry extract.
5. the preparation method of bactericide described in claim 3, is characterized in that, the preparation of described Korean pine polyphenol:
Korean pine is dried, and impurity elimination, pulverizes, sieves;
Ultrasonic extraction, vacuum filtration again, ultrasonic extraction process: ultrasonic temperature 50 DEG C, ultrasonic time 4min, vacuum filtration technique: the centrifugal 10min of 4000rpm rotating speed, gets supernatant;
Described supernatant rotary evaporation in vacuo is obtained concentrate, by concentrate vacuum cooling drying, obtains Korean pine polyphenol.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510376958.5A CN104957190A (en) | 2015-07-01 | 2015-07-01 | Sterilizing agent for livestock breeding as well as preparation method and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510376958.5A CN104957190A (en) | 2015-07-01 | 2015-07-01 | Sterilizing agent for livestock breeding as well as preparation method and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104957190A true CN104957190A (en) | 2015-10-07 |
Family
ID=54211424
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510376958.5A Pending CN104957190A (en) | 2015-07-01 | 2015-07-01 | Sterilizing agent for livestock breeding as well as preparation method and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104957190A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105418251A (en) * | 2015-12-17 | 2016-03-23 | 叶勇 | Anti-caking controlled release fertilizer |
| CN106900751A (en) * | 2015-12-22 | 2017-06-30 | 青岛洁施克林生物科技有限公司 | The sterilization spraying of poultry special bio |
| CN114451426A (en) * | 2022-04-13 | 2022-05-10 | 山东健源生物科技有限公司 | Sterilizing and bacteriostatic biological compound preparation for improving livestock and poultry environment and preparation method thereof |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101028001A (en) * | 2006-02-27 | 2007-09-05 | 权力敏 | Allopelagic sterilizing disinfectant |
| CN102550609A (en) * | 2011-12-20 | 2012-07-11 | 青岛海芬海洋生物科技有限公司 | Sterilization liquid for aquaculture of marine organisms |
| CN102948429A (en) * | 2011-08-30 | 2013-03-06 | 曲奕 | Livin bacteria removing liquid for marine organism |
| CN103110541A (en) * | 2011-11-17 | 2013-05-22 | 王晓薇 | All-natural washing-free disinfectant hand sanitizer |
| CN104042719A (en) * | 2014-07-07 | 2014-09-17 | 江苏瑞京科技发展有限公司 | Antibacterial modified chitosan spray and preparation method thereof |
| CN104311645A (en) * | 2014-01-27 | 2015-01-28 | 北京林业大学 | Spirulina polypeptide P1 with bacteriostatic activity, and application thereof |
-
2015
- 2015-07-01 CN CN201510376958.5A patent/CN104957190A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101028001A (en) * | 2006-02-27 | 2007-09-05 | 权力敏 | Allopelagic sterilizing disinfectant |
| CN102948429A (en) * | 2011-08-30 | 2013-03-06 | 曲奕 | Livin bacteria removing liquid for marine organism |
| CN103110541A (en) * | 2011-11-17 | 2013-05-22 | 王晓薇 | All-natural washing-free disinfectant hand sanitizer |
| CN102550609A (en) * | 2011-12-20 | 2012-07-11 | 青岛海芬海洋生物科技有限公司 | Sterilization liquid for aquaculture of marine organisms |
| CN104311645A (en) * | 2014-01-27 | 2015-01-28 | 北京林业大学 | Spirulina polypeptide P1 with bacteriostatic activity, and application thereof |
| CN104042719A (en) * | 2014-07-07 | 2014-09-17 | 江苏瑞京科技发展有限公司 | Antibacterial modified chitosan spray and preparation method thereof |
Non-Patent Citations (6)
| Title |
|---|
| 李小萍等，: ""红树莓果中鞣花酸提取物的抗氧化性研究"", 《食品科技》 * |
| 章少在等，: ""酶解法制备螺旋藻抗菌肽的研究"", 《黑龙江畜牧兽医》 * |
| 贠可力 等，: ""红松多酚抗氧化实验研究"", 《中国林副特产》 * |
| 辛秀兰等，: ""红树莓果中鞣花酸提取物的抑菌活性初步研究"", 《世界科技研究与发展》 * |
| 郑建仙主编，: "《植物活性成分开发》", 30 June 2005 * |
| 黄雨洋等，: ""红松树皮多酚的提取工艺及其抗氧化活性的研究"", 《生物工程》 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105418251A (en) * | 2015-12-17 | 2016-03-23 | 叶勇 | Anti-caking controlled release fertilizer |
| CN106900751A (en) * | 2015-12-22 | 2017-06-30 | 青岛洁施克林生物科技有限公司 | The sterilization spraying of poultry special bio |
| CN114451426A (en) * | 2022-04-13 | 2022-05-10 | 山东健源生物科技有限公司 | Sterilizing and bacteriostatic biological compound preparation for improving livestock and poultry environment and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104365696A (en) | Chinese herbal medicinal disinfectant for breeding industry | |
| CN102630141A (en) | Antimicrobial composition | |
| Boyko et al. | Changes in the viability of the eggs of Ascaris suum under the influence of flavourings and source materials approved for use in and on foods | |
| Parseh et al. | Antimicrobial properties of Pomegranate (Punica granatum L.) as a Tannin rich Fruit | |
| Singh et al. | Anthelmintic efficacy of aqueous extract of Butea monosperma (Lam.) Kuntze against Haemonchus contortus of sheep and goats | |
| CN104957190A (en) | Sterilizing agent for livestock breeding as well as preparation method and application thereof | |
| KR102171948B1 (en) | Antimicrobial and Antifungal Composition Composition Comprising Mixture of Herb Essential Oil as Active Ingredient | |
| Uushona et al. | Ruminant meat production and quality enhancement, nematode suppression and greenhouse gas emission mitigation: A sustainable paradigm for valorisation of Acacia leaves | |
| KR101335081B1 (en) | The method of producing antibiotic substitute functional feed additive with fermented curcumin, capsacin and apple by-product composite having anti-viral and anti-microbial activity | |
| KR100728722B1 (en) | Antimicrobial and antiviral composition containing natural products | |
| Sorwar et al. | Effect of kalo jeera seeds and papaya leaf supplementation on the performance of broiler. | |
| KR100920713B1 (en) | A composition for the treatment or prevention of diarrhea | |
| Krstić et al. | Antimicrobial activity of sour cherry | |
| KR20180131948A (en) | Pseudomonas extremorientalis strain KACC 81047BP and composition for comprising the same | |
| Kamble et al. | Antimicrobial activity of bioactive herbal extracts against Streptococcus agalactiae biotype 2 | |
| CN101357123A (en) | New antivirus, antibacterial method for animal and plant and product thereof | |
| KR100438209B1 (en) | Complex antimicrobial composition based on carvacrol, thymol, and citral | |
| CN106490022A (en) | A kind of plant compound pesticide containing jervine and preparation method thereof | |
| CN108853314B (en) | Traditional Chinese medicine preparation for preventing and treating frog wry head disease and preparation method and application thereof | |
| Phengvongsone et al. | Antibacterial effect of ethanolic Morus alba Linn. leaf extract against mastitis-causing Escherichia coli and Staphylococcus aureus in vitro. | |
| CN102696899B (en) | A kind of rare bird feed addictive | |
| CN102696897B (en) | A kind of pure natural rare bird feed addictive | |
| CN102715359B (en) | Natural rare bird feed addictive | |
| KR101163559B1 (en) | Feed additive and health food having enhanced immunity and native antibiotics effect | |
| EP3876721A1 (en) | Composition comprising sulphated galactose, and implementations thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20151007 |
|
| RJ01 | Rejection of invention patent application after publication |