CN104945526A - Method for extracting polysaccharide from okra - Google Patents
Method for extracting polysaccharide from okra Download PDFInfo
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- CN104945526A CN104945526A CN201510371953.3A CN201510371953A CN104945526A CN 104945526 A CN104945526 A CN 104945526A CN 201510371953 A CN201510371953 A CN 201510371953A CN 104945526 A CN104945526 A CN 104945526A
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Abstract
The invention discloses a method for extracting polysaccharide from okra, which comprises the following steps: (1) taking fresh tender okra fruit, screening to remove the attachment on the okra surface, weighing, cutting the okra into about 0.5cm*0.5cm dices, filtering to remove okra seeds, adding 4-8 times by weight of water into the pulverized okra, and making the pulverized okra top into a homogenate; (2) filtering the okra homogenate obtained in the step (1), and collecting the filter cake and filtrate, wherein the filtrate is a primary extracting solution; (3) resuspending the okra filter cake obtained in the step (2) in 3-5 times by weight of water, regulating the pH value of the solution to 3.0-6.0, filtering, and collecting the filtrate which is a secondary extracting solution; (4) merging the extraction filtrates, adding proteinase accounting for 0.1-2.0 wt% of the okra, adding calcium chloride accounting for 0.05-1 wt% of the raw material, regulating the pH value to 8-9, and stirring at 30-50 DEG C for 6-10 hours; (5) regulating the pH value of the enzymolysis solution obtained in the step (4) to 3.0-5.5, adding a sulfate solution, stirring uniformly, filtering, and collecting the filtrate; and (6) ultrafiltering the filtrate obtained in the step (5), collecting the trapped fluid, and carrying out spray drying.
Description
Technical field
The invention belongs to field of biological extraction, particularly, the present invention relates to a kind of method extracting polysaccharide from gumbo.
Background technology
Gumbo is Malvaceae (Malvaceae) Abelmoschus annual herb plant, and okra originates in the ground such as Africa Egypt, all has establishing in large scale at present at Europe And Africa.As dish, medicine, flower dual-purpose type plant, its purposes is rather extensive, and in recent years, okra is mainly cultivated to pluck tender pod as vegetables, simultaneously also can in order to prepare solid beverage, alternative tea and polysaccharide product etc.The functional performance of okra is mainly manifested in it to be had higher nutritive value and keeps healthy significantly and food therapy effect two aspects.From nutrition aspect, okra rich in proteins, fat, carbohydrate, VITAMIN, mineral substance and food fibre.Okra is rich in the composition such as flavones, polysaccharide, and simultaneously the content of VITAMIN and mineral substance is also very rich has nourishing function, not only with health role to heart, stomach, skin, also has the efficacy effects such as antifatigue.
The mucoid material that the tender pod of okra contains, it is the mixture by poly semi-lactosi, Polygalactan and arabinan and pectin, wherein pectin is a kind of soluble fiber, in modern health new concept, pole is paid attention to, it can promote the excretion of organic substance in body, effectively can reduce toxin accumulation in vivo, reduce the content of blood sugar and cholesterol; Meanwhile, the degradation product of gumbo polysaccharide is conducive to the Growth and reproduction of probiotic bacterium in enteron aisle, is beneficial to intestinal health.
Research is reported in gumbo mucus in experimentation on animals and can reduces the plasma cholesterol of mouse, but mechanism is failed to understand; Many results of study show the ability of the external conjucated bile acids of multiple fresh vegetables such as gumbo, point out and other vegetables, and as beet, asparagus, green soya bean etc. are compared, gumbo has stronger conjucated bile acids ability, and the cholic acid binding ability impact of boiling on okra is little; Large quantity research shows that the activity research of polysaccharide shows that it has significant conjucated bile acids, reduces the effect of cholesterol in recent years.Meanwhile, gumbo polysaccharide can also as biomaterial, doctor's material device material etc.Although the bibliographical information extracting method of some gumbo polysaccharide, cost is too high, is not suitable for large-scale promotion.
Summary of the invention
The problems such as the not high and Purity of the rate of recovery existed for traditional gumbo Polyose extraction process is low, the present invention aims to provide a kind of method extracting polysaccharide from gumbo, to improve the extraction yield of gumbo polysaccharide, and improves the purity of polysaccharide.A kind of method extracting polysaccharide from gumbo of the present invention comprises the following steps:
(1) pre-treatment: get the tender fruit of fresh gumbo, sieve and remove the dirt settling on gumbo surface, to weigh (weight in wet base) and as payment foundation, gumbo is cut into the fourth shape of about 0.5cm × 0.5cm, filtration method removes gumbo seed, in the gumbo after pulverizing, add the clear water of 4 ~ 8 times of weight, use refiner or colloidal mill that the gumbo of pulverizing top is made into homogenate shape.
(2) once extract: adopt the method for filtering the gumbo homogenate that step (1) obtains to be filtered, collect filter cake and filtrate respectively, filtrate is an extracting solution.
(3) second extraction: gumbo filter cake Eddy diffusion step (2) obtained is in the clear water of 3 ~ 5 times of weight, and regulator solution pH to 3.0-6.0, filtering and collecting filter liquid is secondary raffinate.
(4) enzymolysis: the filtrate merging extracted twice, add the proteolytic enzyme of 0.1 ~ 2.0% gumbo weight, add the calcium chloride of raw material weight 0.05%-1%, adjustment pH is 8-9, within the scope of 30 ~ 50 DEG C, stir 6 ~ 10h.
(5) precipitation process: enzymolysis solution step (4) obtained regulates pH to 3.0 ~ 5.5 and adds sulfate liquor, stirs, uses centrifuging filtrate to be processed, collects filtrate after leaving standstill.
(6) dry: the filtrate that step (5) obtains is carried out uf processing, collect trapped fluid, spraying dry, the powder of acquisition is gumbo polysaccharide.
Preferably, in described step (4) leaching process, the raw material of enzymolysis be through once with second extraction after the consolidated material of filtrate.
Preferably, in described step (4) leaching process, the enzyme of use can be the mixture of papoid, subtilisin or trypsinase or these enzymes.
Preferably, in described step (4) leaching process, the calcium salt added in solution can be solid or the solution of calcium chloride.
Preferably, in described step (5) leaching process, the vitriol added in solution can be ferric sulfate, zinc sulfate or sodium sulfate or used in combination.
Compared with prior art, its advantage comprises in the present invention:
(1) adopt the method for step by step arithmetic, improve the yield of gumbo polysaccharide.
(2) gumbo adopting extracting method of the present invention to prepare has the high advantage of purity.
Specific embodiment
Below by embodiment, the present invention is specifically described; what be worth proposition is; embodiment is just to a kind of explanation of extracting the method for polysaccharide from gumbo of the present invention; the whole of patent of the present invention can not be represented; more limiting the scope of the invention can not be interpreted as; under the precondition of design not departing from essence of the present invention, some adjustment or improvement can also be made, all belong to protection scope of the present invention.
Embodiment 1
(1) pre-treatment: get the tender fruit of fresh gumbo, sieve and remove the dirt settling on gumbo surface, get 1kg and be cut into the fourth shape of about 0.5cm × 0.5cm, screen filtration is adopted to go out gumbo seed, in the gumbo fourth after pulverizing, add the clear water of 6 times of weight, use colloidal mill that the gumbo fourth of pulverizing is made into homogenate shape.
(2) once extract: adopt the method for bag type filtering the gumbo homogenate that step (1) obtains to be filtered, collect filter cake and filtrate respectively, No. one time extracting solution is weighed as 4kg.
(3) second extraction: gumbo filter cake Eddy diffusion step (2) obtained is in the clear water of 4 times of weight, and regulator solution pH to 4.0, filtering and collecting filter liquid, secondary raffinate is weighed as 4kg.
(4) enzymolysis: the filtrate merging extracted twice amounts to 8kg, adds 20g trypsinase, adds the calcium chloride of raw material weight 50g, regulates pH to be 8.0, under 37 DEG C of conditions, stir 6 ~ 10h.
(5) precipitation process: enzymolysis solution step (4) obtained regulates pH to 4.0 and adds sodium sulfate, stirs, uses centrifuging filtrate to be processed, collects filtrate after leaving standstill.
(6) dry: filtrate employing molecular weight cut-off step (5) obtained is that the film of 50kDa carries out uf processing, and collect trapped fluid, carry out spraying dry after concentrating under reduced pressure, the powder of acquisition is gumbo polysaccharide, is weighed as 24g.
Claims (5)
1. from gumbo, extract a method for polysaccharide, it is characterized in that the method comprises the following steps:
(1) pre-treatment: get the tender fruit of fresh gumbo, sieve and remove the dirt settling on gumbo surface, to weigh (weight in wet base) and as payment foundation, by diced for gumbo shape, filtration method removes gumbo seed, in the gumbo after pulverizing, add the water of 4 ~ 8 times of weight, use refiner or colloidal mill that the gumbo of pulverizing top is made into homogenate shape;
(2) once extract: adopt the method for filtering the gumbo homogenate that step (1) obtains to be filtered, collect filter cake and filtrate respectively, filtrate is first time extracting solution;
(3) second extraction: the gumbo filter cake Eddy diffusion that step (2) is obtained in the water of 3 ~ 5 times of weight, regulator solution pH to 3.0-6.0, filtering and collecting filter liquid is second time extracting solution;
(4) enzymolysis: the filtrate merging extracted twice, add the proteolytic enzyme of 0.1 ~ 2.0% gumbo weight, add the calcium chloride of raw material weight 0.05%-1%, adjustment pH is 8-9, within the scope of 30 ~ 50 DEG C, stir 6 ~ 10h;
(5) precipitation process: enzymolysis solution step (4) obtained regulates pH to 3.0 ~ 5.5 and adds sulfate liquor, stirs, uses centrifuging filtrate to be processed, collects filtrate after leaving standstill;
(6) dry: the filtrate that step (5) obtains is carried out uf processing, collect trapped fluid, spraying dry, the powder of acquisition is gumbo polysaccharide.
2. a kind of method extracting polysaccharide from gumbo according to claim 1, is characterized in that in step (4), the raw material of enzymolysis be through once with second extraction after the consolidated material of filtrate.
3. a kind of method extracting polysaccharide from gumbo according to claim 1, is characterized in that, in step (4), the enzyme of use can be the mixture of papoid, subtilisin or trypsinase or these enzymes.
4. a kind of method extracting polysaccharide from gumbo according to claim 1, is characterized in that in step (4), and the calcium salt added in solution can be calcium chloride solution or calcium chloride solid.
5. a kind of method extracting polysaccharide from gumbo according to claim 1, it is characterized in that in step (5), the vitriol added in solution, can be ferric sulfate, zinc sulfate or sodium sulfate or used in combination.
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| CN201510371953.3A CN104945526A (en) | 2015-06-29 | 2015-06-29 | Method for extracting polysaccharide from okra |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105218697A (en) * | 2015-11-13 | 2016-01-06 | 谢镜国 | A kind of method extracting gumbo polysaccharide from okra |
| CN106496353A (en) * | 2016-10-11 | 2017-03-15 | 芜湖职业技术学院 | A kind of Abelmoschus esculentus pectin polysaccharide preparation method for removing hypocholesterolemic activity with high definition |
| CN106699917A (en) * | 2017-01-09 | 2017-05-24 | 中国农业大学 | Ultrasonic-assisted extraction method for polysaccharide extract and pectin extract of okra |
| CN106832027A (en) * | 2016-12-30 | 2017-06-13 | 浙江海洋大学 | The extracting method of gumbo polysaccharide |
| CN107130006A (en) * | 2017-05-19 | 2017-09-05 | 惠州嘉联生物科技开发有限公司 | The fermentation process and its active product of high activity gumbo polysaccharide |
| CN108484785A (en) * | 2018-01-29 | 2018-09-04 | 浙江海洋大学 | A kind of method and monosaccharide component identification method of the extraction purification polysaccharide from gumbo |
| CN110916198A (en) * | 2019-12-24 | 2020-03-27 | 集美大学 | Method for simultaneously preparing pectic polysaccharide and viscous glycoprotein by using okra fermented wine lees |
| CN114686553A (en) * | 2022-04-20 | 2022-07-01 | 湖北瑞邦生物科技有限公司 | Maca and okra peptide composite polysaccharide polypeptide and extraction method and application thereof |
| CN117362465A (en) * | 2023-10-26 | 2024-01-09 | 河北葵克生物科技有限公司 | Method for extracting okra polysaccharide from okra |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101443364A (en) * | 2006-05-10 | 2009-05-27 | 博胡斯生物科技股份公司 | Pectic polysaccharides isolated from fruit pods of okra |
| CN104448033A (en) * | 2014-12-31 | 2015-03-25 | 天津国际生物医药联合研究院 | Extraction method of abelmoschus esculentus pectin |
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| CN101443364A (en) * | 2006-05-10 | 2009-05-27 | 博胡斯生物科技股份公司 | Pectic polysaccharides isolated from fruit pods of okra |
| CN104448033A (en) * | 2014-12-31 | 2015-03-25 | 天津国际生物医药联合研究院 | Extraction method of abelmoschus esculentus pectin |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105218697A (en) * | 2015-11-13 | 2016-01-06 | 谢镜国 | A kind of method extracting gumbo polysaccharide from okra |
| CN106496353A (en) * | 2016-10-11 | 2017-03-15 | 芜湖职业技术学院 | A kind of Abelmoschus esculentus pectin polysaccharide preparation method for removing hypocholesterolemic activity with high definition |
| CN106832027A (en) * | 2016-12-30 | 2017-06-13 | 浙江海洋大学 | The extracting method of gumbo polysaccharide |
| CN106699917A (en) * | 2017-01-09 | 2017-05-24 | 中国农业大学 | Ultrasonic-assisted extraction method for polysaccharide extract and pectin extract of okra |
| CN106699917B (en) * | 2017-01-09 | 2019-04-30 | 中国农业大学 | A kind of method of ultrasound assisted extraction okra polyoses extract and pectin extraction object |
| CN107130006A (en) * | 2017-05-19 | 2017-09-05 | 惠州嘉联生物科技开发有限公司 | The fermentation process and its active product of high activity gumbo polysaccharide |
| CN108484785A (en) * | 2018-01-29 | 2018-09-04 | 浙江海洋大学 | A kind of method and monosaccharide component identification method of the extraction purification polysaccharide from gumbo |
| CN110916198A (en) * | 2019-12-24 | 2020-03-27 | 集美大学 | Method for simultaneously preparing pectic polysaccharide and viscous glycoprotein by using okra fermented wine lees |
| CN114686553A (en) * | 2022-04-20 | 2022-07-01 | 湖北瑞邦生物科技有限公司 | Maca and okra peptide composite polysaccharide polypeptide and extraction method and application thereof |
| CN114686553B (en) * | 2022-04-20 | 2023-11-21 | 湖北瑞邦生物科技有限公司 | Maca okra peptide compound polysaccharide polypeptide and extraction method and application thereof |
| CN117362465A (en) * | 2023-10-26 | 2024-01-09 | 河北葵克生物科技有限公司 | Method for extracting okra polysaccharide from okra |
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