CN104940513A - Pharmaceutical composition for treating menopause syndrome - Google Patents

Abstract

The present invention discloses a pharmaceutical combination for treating menopause syndrome, which comprises the following raw materials in weight part: 120 parts of prepared rehmannia root, 120 parts of Curculigo orchioides, 120 parts of Chinese yam, 120 parts of Cornus officinalis, 120 parts of epimeddium, 120 parts of salted morinda officinalis, 120 parts of taxillus, 120 parts of ligustrum lucidum, 120 parts of yerbadetajo herb, 100 parts of Chinese angelica, 90 parts of cassia twig, 90 parts of levisticum, 90 parts of Saposhnikovia divaricata, 30 parts of Asarum Herb and 60 parts of licorice. The pharmaceutical combination is effective in treating kidney yin-yang deficiency type premenopausal and postmenopausal diseases for women and can significantly improve the symptoms, the physical signs and the related indexes of premenopausal and postmenopausal diseases. The effect of the pharmaceutical combination is superior to that of Youguiwan decoction and the pharmaceutical combination is an effective, simple, convenient and safe Chinese materia medica preparation in treating premenopausal and postmenopausal diseases.

Description

The syndromic drug regimen for the treatment of postmenopausal women

Technical field

The present invention relates to one and treat the syndromic medicine of postmenopausal women, belong to technical field of Chinese medicine.

Background technology

Menopausal syndrome belongs to traditional Chinese medical science menopausal syndrome category, be women before and after menopause, kidney qi degradation, Radix Semiaquilegiae gradually exhausts, and rushes wantonly two deficient pulses and declines.To suffer from a deficiency of the kidney, can because of the relatively deficiency of YIN, relatively yang deficiency or deficiency in both YIN and YANG and occur different disease, and can the heart be involved, liver, spleen.

This sick Western medicine diagnose standard meets the women of " perimenopausal syndrome " of World Health Organization's proposition in 1994.Climacteric refers to one period around menopause, comprises from close to the menopause appearance endocrine relevant with menopause, and biology and Clinical symptoms to rise to last menstruation 1 year, namely after menopause transition to last menstruation 1 year.Perimenopausal syndrome refer to before and after postmenopausal women due to gonadal hormone reduce caused by a series of body and psychic symptoms.

Tcm diagnosis standard is with reference to " new Chinese medicine guideline of clinical investigations " the 3rd volume " new Chinese medicine treatment female dimacteric syndrome guideline of clinical investigations " and meet national higher medical institution sixth version textbook, the kidney yin and yang deficiency type primary symptom of " Gynecology of Chinese Medicine " menopausal syndrome is shown in: symptom coldness of the body with chills, Tidal fever with perspiration, dim complexion, emotional, insomnia, tired, nocturia frequency, Musculoskeletal pain, light red tongue, white, slippery and moist fur, deep-thready pulse; Secondary disease: puffiness of the face and limbs, dizzy, cardiopalmus, depression, sexual anhedonia; Picture of the tongue light red tongue, white, slippery and moist fur; Pulse condition deep-thready pulse.

Chinese medicine primary disease, focuses on integrally-regulated, based on the kidney invigorating, holds concurrently and adjusts that he is dirty, treating both the principal and secondary aspects of a disease, reaching " YIN and YANG in a relative equilibrium, spirit and even " object.Except focusing on negative and positive in Heibei provincial opera kidney (deficiency of the kidney yin---nourishes kidney yin, help with YANG hyperactivity suppressing, insufficiency of kidney-YANG---the kidney warming invigorating YANG, kidney insufficiency---tonifying both YIN and YANG) in treatment.Or nourishing the liver and kidney, restoring normal coordination between the heart and kidney, the kidney warming warms up spleen, or depressed liver-energy dispersing and QI regulating is reduced phlegm.But by long-term clinical observation treatment, find that still ascribing to of menopausal syndrome is suffered from a deficiency of the kidney, namely the void of kidney qi declines at all.And single with the kidney invigorating warming YANG YIN nourishing unsatisfactory curative effect to the patient of insufficiency of kidney-YANG and deficiency in both YIN and YANG, clinical observation finds kidney yang deficiency then impatency meridians, makes yang-energy not reach the disease occurring wind and cold impatency, and with the kidney invigorating warming YANG, the every common effect of expelling wind and cold method treatment.Therefore, with Chinese medicine the kidney invigorating warming YANG, expelling wind and cold is main treatment postmenopausal women syndrome is the technical background that the present invention proposes.

Summary of the invention

Technical problem to be solved by this invention is to provide one and treats the syndromic drug regimen of postmenopausal women (the kidney invigorating dispel the wind climacteric mixture), to realize adopting the syndromic object of Chinese medicine postmenopausal women.

Technical scheme of the present invention is such:

The kidney invigorating of the present invention mixture that dispels the wind is made up of the crude drug of following parts by weight: Radix Rehmanniae Preparata 114 ~ 132 parts, Rhizoma Curculiginis 114 ~ 132 parts, Rhizoma Dioscoreae 114 ~ 132 parts, Fructus Corni 114 ~ 132 parts, Herba Epimedii 114 ~ 132 parts, 114 ~ 132 parts, stir-baking RADIX MORINDAE after sprinking salt solution sky, Herba Taxilli 114 ~ 132 parts, wine Fructus Ligustri Lucidi 114 ~ 132 parts, Herba Ecliptae 114 ~ 132 parts, Radix Angelicae Sinensis 95 ~ 110 parts, Ramulus Cinnamomi 94.5 ~ 99 parts, Radix Angelicae Pubescentis 94.5 ~ 99 parts, Radix Saposhnikoviae 95 ~ 99 parts, Herba Asari 31.5 ~ 33 parts, 57 ~ 66 parts, Radix Glycyrrhizae.Adjuvant: ethyl hydroxybenzoate 0.5 part.

Radix Rehmanniae Preparata enriching yin and nourishing kidney, life essence-filling, marrow-benefitting in side; Rhizoma Dioscoreae supplementing QI and nourishing YIN; Fructus Corni invigorating liver and spleen; Three medicines match and form " three mend " classical in LIUWEI DIHUANG WAN, in order to nourishing the liver and kidney; Rhizoma Curculiginis, Herba Epimedii, stir-baking RADIX MORINDAE after sprinking salt solution sky warming the kidney to invigorate YANG, bone and muscle strengthening, Eradicates rheumatism; Chinese angelica blood supplementing is invigorated blood circulation; Herba Ecliptae, wine Fructus Ligustri Lucidi the kidney invigorating nourishing YIN; Ramulus Cinnamomi diaphoresis expelling pathogenic factors from muscles promoting the flow of QI-blood by warming the meridian, supporing yang activating QI; Radix Angelicae Pubescentis, Radix Saposhnikoviae deliver loose wind, and part of the body cavity below the umbilicus, housing the bladder, kidneys and bowels damp of dispelling is fought bitterly; Herba Taxilli invigorating the liver and kidney, bone and muscle strengthening; The pungent temperature of Herba Asari is dispersed, and warm ruton is strange; The invigorating middle warmer of Radix Glycyrrhizae QI invigorating, coordinating the actions of various ingredients in a prescription.Full side is with the kidney invigorating, and in mediation kidney, negative and positive are main, and the dehumidifying medicine of cooperation functions dispelling wind and clearing away cold, removing dampness dredging collateral to treat, suffer from a deficiency of the kidney, kidney imbalance of YIN and YANG by all diseases before and after menopause, the card of meridians impatency.Before and after treatment menopause, in all diseases, there is the rule for the treatment of holding the kidney invigorating, pass the chief that dredging collateral is eliminating evil, raise the particular views of the theory for the treatment of in accordance with local conditions.

The dispel the wind preparation method of mixture of the kidney invigorating of the present invention is as follows:

By ten five tastes crude drug in prescription, decoct with water 3 times, first time adds 10.5 times of water gagings (namely adding the water of 10.5 times of weight of all crude drug gross weights), and second and third time respectively adds 8 times of water gagings, and each decoction 0.5 hour, filters, merging filtrate; Filtrate normal pressure concentrates, and adds ethyl hydroxybenzoate (using a small amount of dissolve with ethanol), and mixing, adds purified water, stir evenly, subpackage, to obtain final product.

prescription drug physicochemical property and pharmacological research:

radix Rehmanniae Preparata(scrophulariaceae rehmannia glutinosa plant rehmannia glutinosathe Preparation process product of dried root Libosch.): the main chemical compositions of Radix Rehmanniae tuber is iridoid glycosides.Radix Rehmanniae Preparata is owing to heating vexed steaming for a long time, and iridoid glycoside constituents is relatively few, is about 1/10 of Radix Rehmanniae.Radix Rehmanniae is separated other glycosides obtained to be had: digitophyllin C, echinacoside, burnt Radix Rehmanniae phenethyl alcohol glycoside A ₁, B ₁, cistanoside A, F and stamen flower glucosides etc.

Radix Rehmanniae Preparata, sweet, tepor.Return liver, kidney channel.Have YIN nourishing of enriching blood, beneficial essence fills out effect of marrow.For blood deficiency and yellow complexion, severe palpitation, menoxenia, metrostaxis, the hepatic and renal YIN deficiency, soreness of the waist and knees, osteopyrexia and fever, night sweat is passed out semen, and interior-heat is quenched one's thirst, dizzy, tinnitus, early whitening of beard and hair.

Modern pharmacological research shows, Radix Rehmanniae Preparata mainly contains the pharmacological action of following three aspects: 1. on the impact of blood system: prepared rehmannia root polysaccharide gavage to resembling through cyclophosphamide or radio-induced blood deficiency mouse peripheral blood, bone marrow nucleated cell declines all has antagonism, can promote increment and the differentiation of mouse hematopoietic stem cell; 2. on immune impact: oral Dihuang polysaccharide can make the phagocytic percentage of caused by cyclophosphamide immunosuppressed mice peritoneal macrophage and phagocytic index significantly raise; 3. on the impact of cardiovascular system: Radix Rehmanniae decoct oral administration obviously can resist the ischemia/reperfusion in rats myocardial hypertrophy that Levothyroxinnatrium brings out, and the heart, brain mitochondria Ca can be suppressed ²⁺, Mg ²⁺-atpase activity, thus protection heart and brain tissues avoids ATP to exhaust and ischemic injuries.

In conjunction with the characteristic that the effective ingredient of Radix Rehmanniae Preparata, pharmacological research, clinical practice and function cure mainly, Radix Rehmanniae Preparata adopts the preparation technology of water boiling and extraction in the present invention.

rhizoma Curculiginis(amrallid Rhizoma Curculiginis curculigo orchioidesgaertn. dry rhizome): Rhizoma Curculiginis rhizome is containing cycloartane type triterpene and glucoside compound thereof: curculigenin A, B, C, Rhizoma Curculiginis saponin A, B, C, D, E, F, G, H, I, J, K, L, curculigol, also contains the glucoside compound of methylphenol and oxomethyl phenol, curculigoside, lichens glycol glucoside, curculigineA, B, C, curculigoside B, also contains Cycloartane-type triterpene glycosides constituents.

Rhizoma Curculiginis, pungent, heat; Poisonous.Return kidney,liver,spleen warp.There is kidney-replenishing, bone and muscle strengthening, effect of dispelling cold and dampness.For impotence and seminal emission, muscles and bones flaccidity is soft, chills and pain of the waist and kness, yang deficiency cold diarrhea.

Modern pharmacological research shows, Rhizoma Curculiginis mainly contains the pharmacological action of following seven aspects: 1. adaptive significances: curculigol preserved material, to mouse gavaging, can obviously extend the mice oxytolerant time-to-live.Curculigol preserved material has high temperature resistance effect to mouse peritoneal injection; 2. defying age: Rhizoma Curculiginis can extend mouse survival number and mean survival time, and the lipofuscin content that obviously can reduce the heart, brain; 3. immune enhancing function: Rhizoma Curculiginis electricity sugar is external can stimulate spleen lymphocyte proliferation separately, reduces the suppressed mouse thymus of HC induction of immunity and spleen weight, and thymocyte cell and splenic T, B cell proliferation reduce, and have obvious inhibitory action; 4. osteoporosis: have obvious facilitation to the propagation of bone like cell; 5. calm, convulsion: its alcohol preserved material has obvious sedation, mouse peritoneal injection obviously can extend the length of one's sleep that pentobarbital sodium causes, and also obviously can delay the mice battle array twin property convulsions time of occurrence that Picrotoxin causes; 6. antiinflammatory: lumbar injection Rhizoma Curculiginis alcohol extraction agent has obvious inhibitory action to Oleum Tiglii induced mice auricle edema; 7. other: its water decoction can significantly improve Na ⁺-K ⁺-atpase activity, the effects such as coronary artery dilator, heart tonifying, quickening heart rate.

In conjunction with the characteristic that the effective ingredient of Rhizoma Curculiginis, pharmacological research, clinical practice and function cure mainly, Rhizoma Curculiginis adopts water boiling and extraction to be rational in we.

rhizoma Dioscoreae(Dioscoreaceae plant Rhizoma Dioscoreae dioscorea oppositathunb. dry rhizome) to study point: containing Chinese yam polysaccharide, dopamine, hydrochloric acid Rhizoma Dioscoreae alkali, polyphenol oxidase in its rhizome, and containing multiple sterols composition Dihydrocholesterol, cholesterol and derivant thereof; In recent years be also separated and obtain new glucosides, still containing polysaccharide RDRS-1.

Rhizoma Dioscoreae, sweet, flat.Return spleen, lung, kidney channel.Have spleen reinforcing nourishing the stomach, promote the production of body fluid lung benefiting, effect of the kidney invigorating arresting seminal emission.For insufficiency of the spleen lack of appetite, incessant chronic diarrhea, cough and asthma due to lung deficiency, emission due to deficiency of the kidney, leukorrhagia, frequent micturition, deficiency-heat is quenched one's thirst.

Modern pharmacological research shows, Rhizoma Dioscoreae mainly contains the pharmacological action of following four aspects: pharmacologically active: 1. adjust gastrointestinal motility: gastric emptying can be suppressed to move, can antagonism acetylcholine, Ileum From A White tetanic contraction effect caused by barium chloride, and have inhibitory action to the enhancing of neostigmine load Mouse Stomach intestine movement; 2. immunoloregulation function: Chinese yam polysaccharide can improve the immunomodulating of specific cellular immunity and body fluid, also has non-specific immunity; 3. blood sugar lowering: its water decoction is to mouse stomach, the blood glucose of normal mouse can be reduced, preventive and therapeutic action is had to the diabetes mice caused by alloxan, also significant inhibitory action is had to the mouse blood sugar rising that epinephrine and glucose cause, Chinese yam polysaccharide is hypoglycemic effective ingredient, its effect with increase insulin secretion, to improve impaired islet beta cell function relevant; 4. other: its water decoction, to mouse gavaging, can increase its prostate, weight of seminal vesicle, produce Andrin-like action, the concrete In Vitro Anti mutation effect of Chinese yam polysaccharide.

In conjunction with the characteristic that mountain the effective elements of the medicine, pharmacological research, clinical practice and function cure mainly, Rhizoma Dioscoreae adopts the preparation technology of water boiling and extraction in the present invention.

fructus Corni(Cornaceae plant Fructus Corni cornus officinalissieb. the drying and ripening sarcocarp of et Zucc.): mainly contain 1. iridoid glycoside constituents in its sarcocarp: the new glycosides of meliatin, cornin, Fructus Corni; 2. triterpenoid compound: ursolic acid, oleanolic acid; 3. tannin constituents: Fructus Corni tannin , , Herba Diclipterae Chinensis tannin D, Fructus seu radix camptothecae acuminatae (Fructus Camptothecae Acuminatae) tannin A, B; 4. Determination of Organic Acids: tartaric acid, malic acid, citric acid, gallic acid, succinic acid; Other are also containing compositions such as skunk bush polysaccharides.

Fructus Corni, sour, puckery, tepor.Return liver, kidney channel.There is liver and kidney tonifying, arresting collape syndrome by astringents.For vertigo and tinnitus, soreness of waist and knee joint, impotence and seminal emission, enuresis frequent micturition, bleeding not during menses, profuse sweating is collapsed, and interior-heat is quenched one's thirst.

Modern pharmacological research shows, Fructus Corni mainly contains the pharmacological action of following five aspects: 1. immunoloregulation function: its water decoction intraperitoneal administration makes the obvious atrophy of mouse thymus, slow down the Cl of reticuloendothelial cell to carbon granule, also mice serum hemolytic antibody content and serum anti-immunoglobulin G content can be raised, demonstrate immunoregulation effect, Fructus Corni total glycosides can obviously suppress mouse lymphocyte to transform and LAK Hemapoiesis in vitro, take the generation that can suppress IL-2 in body, have immunosuppressive action; 2. blood pressure lowering: Fructus Corni decoct can reduce the blood sugar level of alloxan diabetes rats, and liver glycogen content can be increased, its iridoid constituents can reduce Streptozocin blood glucose in diabetic rats level, and the heart cause diabetes and nephropathy also have protective effect; 3. antiinflammatory: the mouse peritoneal capillary permeability that Fructus Corni water decoction Dichlorodiphenyl Acetate causes increases, rat granuloma hamartoplasia, the inflammation such as the rat toes swelling that the mice auricle swelling caused by dimethylbenzene and Ovum Gallus domesticus album cause have inhibitory action, experiment in vivo proves, Fructus Corni total glycosides has good antiinflammatory action; 4. antibacterial: experiment in vitro proves, its decoct has inhibitory action to funguses such as staphylococcus aureus, dysentery bacterium and Dong's color hair moss bacterium, and the many Bacillus typhi of fresh fruit meat and dysentery bacterium have inhibitory action; 5. other: skunk bush polysaccharide has antioxidant activity, protects the liver in addition, the effect such as antitumor and anti-defective virus.

In conjunction with the characteristic that the effective ingredient of Fructus Corni, pharmacological research, clinical practice and function cure mainly, Fructus Corni adopts the preparation technology of water boiling and extraction in the present invention.

herba Epimedii(Berberidaceae plant Herba Epimedii epimedium brevicornumaxim., Epimedium sagittatum epimedium sagittatum(Sieb. et Zucc.) Maxim., E. Pubescens epimedium pubescensor Herba Epimedii Maxim. epimedium koreanumthe dried leaves of Nakai): its aerial parts mainly contains flavones ingredient, as: icariin, epimedoside, kaempferol-3,7-O-α-L-two rhamnoside, β-dehydration icariin etc.

Herba Epimedii, acrid, sweet, warm.Return liver, kidney channel.There is kidney-replenishing, bone and muscle strengthening, effect of wind-damp dispelling.For kidney yang deficiency, impotence and seminal emission, muscles and bones flaccidity is soft, rheumatic arthralgia, numbness contracture.

Modern pharmacological research shows, Herba Epimedii mainly contains the pharmacological action of following five aspects: 1. promote gonad function: the extraction position of Herba Epimedii water decoction is that dosage makes the corpus cavernosal smooth muscle of phyenlephrinium or electricity irritation process relax and increase toleration according to patience, increases the generation of cyclic GMP in L-arginine level and mice cavernosal tissue simultaneously; 2. promoting bone growing: study mechanism display Herba Epimedii total flavones can by protection gonad, suppress the mrna expression of bone resorption and osteocyte interleukin-6 and tumor necrosis factor α, the mrna expression of promoting bone growing and osteocyte transforming growth factor beta 1, thus prevent osteoporosis; 3. affect cardiovascular system: Herba Epimedii extract can reduce anesthetized dog Total peripheral vascular resistance and left room EDP, increase coronary artery blood flow, cardiac output, pulse output etc., Herba Epimedii water extract has calcium antagonistic activity simultaneously; 4. immunoloregulation function: Herba Epimedii energy enhancing body non-specific immunity and cellular immune function, regulate humoral immune function, effective ingredient is mainly icariin and epimedium brevicornum polysaccharide.Epimedium brevicornum polysaccharide and Herba Epimedii total flavones all can significantly improve macrophage to the phagocytic rate of chicken red blood cell and phagocytic index, can significantly reduce by antagonism caused by cyclophosphamide mouse monokaryon macrophage phagocytic function.To macrophages secrete interleukin-1 (IL-1) and anti-tumor factor (TNF), there is dual regulation; 5. other: its extract and icariin have antidepressant effect, and Herba Epimedii total flavones also has antiinflammatory action.

In conjunction with the characteristic that the effective ingredient of Herba Epimedii, pharmacological research, clinical practice and function cure mainly, Herba Epimedii adopts the preparation technology of water boiling and extraction in the present invention.

stir-baking RADIX MORINDAE after sprinking salt solution sky(Maguireothamnus speciosus Radix Morindae Officinalis morinda officinalisthe processed product of the dry root of How): the root of Radix Morindae Officinalis mainly contains 1. flavones ingredient, as: 1,6-dihydroxy-2,4-dimethoxy anthraquinone, Rubiadin, physcione; 2. iridoid and glycoside thereof: 10-deacetyl asperulosidic glycosides; There is the oligosaccharides of antidepressant activity: resistance to these sugar, seven polysaccharide; 3. cyclopropanone analog derivative: Radix Morindae Officinalis element.

Stir-baking RADIX MORINDAE after sprinking salt solution sky, sweet, pungent, tepor.Return kidney, Liver Channel.There is kidney-replenishing, bone and muscle strengthening, effect of wind-damp dispelling.For impotence and seminal emission, cold womb is infertile, menoxenia, and few abdomen cold type of pain, rheumatic arthralgia, muscles and bones flaccidity is soft.

Modern study pharmacology shows, stir-baking RADIX MORINDAE after sprinking salt solution sky mainly contains the effect of following five aspects: 1. improve body function, strengthen immunity: its water decoction significantly can strengthen Mouse Weight to mouse stomach, extend swimming time, improve anti-fatigue ability, polysaccharides from morinda officinalis how gavage can increase young mice thymic weight, improve the phagocytic activity that spleen produces immune special rosette forming cell and macrophage, oligosaccharide is external can strengthen mouse spleen cell proliferation ability, strengthens mouse boosting cell antibody forming cell number.Aqueous extracts gavage can improve S ₁₈₀the immunologic function of tumor-bearing mice, regulates and improves body and erythrocytic immunologic function; 2. on the impact of reproductive function: Radix Morindae Officinalis decocting liquid gavages to female rats, ovary, uterus and hypophysis weight can be made to increase, and the function increasing human chorionic gonadotropin in ovary/metakentrin receptor can also improve the reaction of ovary to metakentrin, ovulation rate corpus luteum generates and maintains luteal function, male mice gavages decocting liquid can to the first cell of essence and primary spermatocyte generation effect, remarkable reduction rate of teratosperm, the lipid peroxidation injury of its water extract to Human sperm membrane has intervention effect significantly, obviously protection sperm membrane 26S Proteasome Structure and Function; 3. on the impact of skeletal system: the core binding factor alpha that Aqueous extracts and alcohol extract gavage can make bone cell differentiation relevant ₁express and strengthen, promote that marrow stromal cell is to osteoblast differentiation, thus promoting bone growing; 4. on the impact of myocardial cell: its Aqueous extracts gavage also has protective effect to myocardial ischemia-reperfusion injury; 5. protect the liver: its decocting liquid gavage can weaken Acute Liver Injury Induced by carbon tetrachloride model mice cell damage degree, obviously can also suppress the growth of mouse bearing liver cancer tumor.

In conjunction with the characteristic that the effective ingredient in stir-baking RADIX MORINDAE after sprinking salt solution sky, pharmacological research, clinical practice and function cure mainly, stir-baking RADIX MORINDAE after sprinking salt solution sky adopts the preparation technology of water boiling and extraction in the present invention.

herba Taxilli(Loranthaceae plant Herba Taxilli taxillus chinensis(DC.) the dry stem and branch with leaf of Danser): main containing Herba Taxilli toxalbumin, agglutinin of loranthyl; Flavones ingredient: avicularin, Quercetin, Quercitroside, also containing d-catechin etc.

Herba Taxilli, bitter, sweet, flat.Return liver, kidney channel.There is wind-damp dispelling, invigorating the liver and kidney, bone and muscle strengthening, effect of antiabortive unit.For rheumatic arthralgia, soreness of the waist and knees, muscles and bones is unable, and metrorrhagia is through many, and blood leaking in gestation, frequent fetal movement, has a dizzy spell.

Modern pharmacological research shows, Herba Taxilli mainly contains the pharmacological action of following three aspects: 1. on the impact of cardiovascular system: a. blood pressure lowering: its water logging agent and alcohol preserved material have hypotensive effect, active component is mainly avicularin, and rat tail vein injection water decocting liquid also shows transient hypotensive effect; B. blood fat reducing: after Herba Taxilli defat, the ethanolic extract of decocting liquid has the effect of obvious cholesterol reducing and triacylglycerol to hyperlipidemia rats, can significantly improve SOD activity, reduces LPO in serum; 2. on immune impact: its decocting liquid and granule gavage thereof have obvious inhibitory action to 2,4-bis-nitrilo benzene induced mice skin of pinna delayed hypersensitivity; 3. antiinflammatory, analgesia: Herba Taxilli decocting liquid and the reaction of the mouse writhing caused by granule gastric infusion Dichlorodiphenyl Acetate thereof have obvious inhibitory action, and on Carrageenan causes that the swelling of rat toes also has inhibitory action.

In conjunction with the characteristic that the effective ingredient of Herba Taxilli, pharmacological research, clinical practice and function cure mainly, Herba Taxilli adopts the preparation technology of water boiling and extraction in the present invention.

wine Fructus Ligustri Lucidi(Oleaceae plants Fructus Ligustri Lucidi ligustrum lucidumthe processed product of dry mature fruit Ait.): mainly contain 1. triterpenes components, as: oleanolic acid, ursolic acid, 19 Alpha-hydroxies-acetyl ursolic acid etc.; 2. secoiridoid, as: the acid of nuzhenide, Fructus Ligustri Lucidi, Fructus Ligustri Lucidi fruit A, B, C, D, Nuezhengslaside etc.; 3. flavonoid, as: Quercetin, apigenin-7-O-beta-D-glucopyranose glucosides etc.; 4. containing Phenylpropanoid Glycosides methods of glycosides, as Fructus Ligustri Lucidi element, ligustrin etc.

Wine Fructus Ligustri Lucidi, sweet, bitter, cold.Return liver, kidney channel.There is nourishing the liver and kidney, effect of improving eyesight black hair.For the hepatic and renal YIN deficiency, vertigo and tinnitus, soreness of the waist and knees, early whitening of beard and hair, poor vision, interior-heat is quenched one's thirst, osteopyrexia and fever.

Modern pharmacological research shows, wine Fructus Ligustri Lucidi mainly contains the pharmacological action of following eight aspects: 1. strengthen immunity: the cellular immunization of water decoction to the nonspecific immunity of normal mouse stomach and caused by cyclophosphamide immunosuppressed mice has potentiation, obvious facilitation is had, the external ability that also can improve normal mouse T lymphocytic emiocytosis interleukin-2 to the T lymphocyte of deficiency of YIN mice spleen caused by adrenocortical hormone; 2. antioxidation, defying age; 3. hair follicle growth is promoted: the mice antenna hair follicle of decocting liquid to In vitro culture has growth promoting effects effect significantly; 4. melanin formation promoting; 5. protect the liver: oleanolic acid and Glossy Privet Fruit Wine process product decocting liquid gavage has significant protective effect to the large and small Mus acute liver damage that carbon tetrachloride causes, and serum glutamic pyruvic transminase can be made obviously to decline; 6. antitumor; 7. blood sugar lowering: its water decoction gavage increases normal mouse blood sugar and the mouse blood sugar that caused by alloxan, epinephrine and glucose all facilitation significantly, and oleanolic acid is hypoglycemic main component; 8. other: atherosclerosis, antiinflammatory, antibacterial etc.

In conjunction with the characteristic that the effective ingredient of wine Fructus Ligustri Lucidi, pharmacological research, clinical practice and function cure mainly, wine Fructus Ligustri Lucidi adopts the preparation technology of water boiling and extraction in the present invention.

herba Ecliptae(feverfew Eclipta prostrata eclipta prostratal. dry aerial parts): mainly contain 1. triterpenoid saponins: Fructus seu radix camptothecae acuminatae (Fructus Camptothecae Acuminatae) glycosides A, B, C, D, sweet wine intestinal saponin - ; 2. triterpenes components: echinocystic acid, oleanolic acid; 3. thiophenes: α-terthienyl; 4. coumarin kind compound; 5. flavonoid: Quercetin, apigenin etc.; 6. volatile oil: epoxy caryophyllene etc.

Herba Ecliptae, sweet, sour, cold.Return kidney, Liver Channel.There is nourishing the liver and kidney, effect of cooling blood for hemostasis.For the hepatic and renal YIN deficiency, odontoseisis, early whitening of beard and hair, vertigo and tinnitus, soreness of the waist and knees, YinXuXueRe haematemesis, epistaxis, hematuria, dysentery, metrostaxis, traumatic hemorrhage.

Modern pharmacological research shows, Herba Ecliptae mainly contains the pharmacological action of following eight aspects: 1. stop blooding: decocting liquid gavage obviously can shorten gastrorrhagia mice and coagulate former zymogen time and partial prothrombin time, raise fibrinogen content and platelet counts, reduce gastric bleeding to count, have significant anastalsis; 2. antiinflammatory: decocting liquid gavage is to the mice auricle swelling caused by Oleum Tiglii, rat toes swelling caused by carrageenin or formaldehyde, the mouse peritoneal capillary permeability that acetic acid causes increases, the rat skin capillary permeability that histamine causes increases, and rat granuloma hamartoplasia only has inhibitory action significantly; 3. antidote against snake bite; 4. protect the liver; 5. on the impact of immunologic function: mice delayed hypersensitivity and serum antihemolysin antibody horizontal can be significantly improved, the Mouse Thymocytes Apoptosis of cyclophosphamide induction is suppressed; 6. on the impact of cardiovascular system; 7. antimutagenic: water decoction gavage or lumbar injection, has obvious inhibitory action, tool antimutagenic activity to the mouse bone marrow cells Polychromatic erythrocytes micronucleus of Induced by Cyclophosphamide; 8. other: antibacterial, raise peripheral leukocytes etc.

In conjunction with the characteristic that the effective ingredient of Herba Ecliptae, pharmacological research, clinical practice and function cure mainly, Herba Ecliptae adopts the preparation technology of water boiling and extraction in the present invention.

radix Angelicae Sinensis(umbelliferae angelica angelica sinensis(OLiv.) dry root of Diels): angelica root is containing volatile oil and involatile constituent.Herb each several part all contains volatile oil, and is many with fruit content.In oil, main component is positive fourth alkene Fu lactone and Rhizoma Ligustici acid esters, and the former is the composition with special aroma; Separately there are the positive pentanone of adjacent carboxyl benzene and sesquiterpene etc.

Radix Angelicae Sinensis, sweet, acrid, warm.Return liver, the heart, spleen channel.Having enriches blood invigorates blood circulation, menstruction regulating and pain relieving, effect of loosening bowel to relieve constipation.For blood deficiency and yellow complexion, dizzy cardiopalmus, menoxenia, amenorrhea dysmenorrhea, asthenia cold abdominalgia, rheumatic arthralgia, injury from falling down, ulcer sores, dryness of the intestine constipation.

Modern pharmacological research shows, Radix Angelicae Sinensis mainly contains the pharmacological action of following five aspects: 1. anti-thrombosis function: Radix Angelicae Sinensis water preparation intravenous injection or be orally formed with obvious inhibitory action to rat artery and venous bypass thrombosis; 2. blood circulation effect is improved; 3. to the effect of cardiovascular system: Radix Angelicae Sinensis decoct or fluid extract have inhibitory action to isolated mouse heart, heart beating when dosage is large, can be made to stop at relaxing period.The arrhythmia of Radix Angelicae Sinensis to rat myocardial ischemia and reperfusion has protective effect, and Radix Angelicae Sinensis injection 0.6 g/Kg is used for rats by intraperitoneal injection, and premature beat incidence rate and the total incidence rate of arrhythmia can be made obviously to reduce; 4. improve the effect of coronary circulation: intravenous injection Radix Angelicae Sinensis decoct (2 g/kg) significantly can increase coronary flow, reduce coronary resistance and myocardial oxygen consumption, increase heart output and SI, alleviate myocardial infarct size when blocking arteria coronaria; 5. to the effect of level and smooth flesh: Radix Angelicae Sinensis volatile oil is that Radix Angelicae Sinensis plays the dominant reactive part of spasmolysis to vascular smooth muscle, and wherein ligustilide activity is the strongest.Radix Angelicae Sinensis volatile oil also has stronger inhibitory action to intestinal smooth muscle spasm.

In conjunction with the characteristic that the effective ingredient of Radix Angelicae Sinensis, pharmacological research, clinical practice and function cure mainly, Radix Angelicae Sinensis adopts the preparation technology of water boiling and extraction in the present invention.

ramulus Cinnamomi(canella Cortex Cinnamomi cinnamomum cassiathe dry twig of Presl): Ramulus Cinnamomi is containing volatile oil, and in oil, main component is cinnamic aldehyde, also has benzyl benzoate, cinnamyl acetate, β-cadinene, Chang Pu alkene, coumarin etc.

Ramulus Cinnamomi, acrid, sweet, warm.GUIXIN, lung, urinary bladder channel.There is diaphoresis expelling pathogenic factors from muscles, promoting the flow of QI-blood by warming the meridian, supporing yang activating QI, effect of the flat gas that spins.For anemofrigid cold, coldness and pain in the epigastrium, cold in blood amenorrhea, arthralgia, phlegm retention, edema, cardiopalmus, renal mass.

Modern pharmacological research shows, Ramulus Cinnamomi mainly contains the pharmacological action of following two aspects: 1. antibacterial action: Ramulus Cinnamomi ethanol extract can suppress escherichia coli, bacillus subtilis and staphylococcus aureus in vitro, and valid density is 25 mg/mL or following; Suppression is all had to Staphylococcus albus, dysentery bacterium, Typhoid and paratyphoid bacillus, streptococcus pneumoniae, aerobacteria, Bacillus proteus, anthrax bacillus etc.; 2. antivirus action: Ramulus Cinnamomi decoct infected by influenza has inhibitory action.

In conjunction with the characteristic that the effective ingredient of Ramulus Cinnamomi, pharmacological research, clinical practice and function cure mainly, Radix Angelicae Sinensis adopts the preparation technology of water boiling and extraction in the present invention.

radix Angelicae Pubescentis(samphire Angelica pubescens angelica pubescensmaxim. f. biserratathe dry root of Shan et Yuan): mainly containing coumarin kind compound, as: bergapton.Osthole etc.; Also containing volatile oil: thymol, p-methylphenol etc.

Radix Angelicae Pubescentis, pungent, bitter, tepor.Return kidney, urinary bladder channel.There is expelling wind and removing dampness, effect of numbness relieving and pain relieving.For anemofrigid-damp arthralgia, lumbocrural pain, few cloudy volt wind headache, wind and cold holds wet headache under the arm.

Modern pharmacological research shows, Radix Angelicae Pubescentis mainly contains the pharmacological action of following three aspects: 1. antiinflammatory, analgesia and sedation; 2. inhibitory action is had to platelet aggregation; 3. hypotensive effect, but not lasting, and contained bergapten, xanthotoxin etc. have photosensitive and antitumor action.

In conjunction with the characteristic that the effective ingredient of Radix Angelicae Pubescentis, pharmacological research, clinical practice and function cure mainly, Radix Angelicae Pubescentis adopts the preparation technology of water boiling and extraction in the present invention.

radix Saposhnikoviae(umbelliferae Saposhnikovia divaricata saposhnikovia divaricata(Turcz.) dry root of Schischk.): opoponax mainly contains 1. chromone constituents: Radix Saposhnikoviae chromone alcohol, 4 '-O-glucosyl group-5-O-methyl tooth A Mi, 3 '-O-angeloyl groups last of the twelve Earthly Branches phenol, hamaudol, 3 '-O-acetyl group hamaudol, hamaudol glycoside, 5-O-methyl tool ammiol, cimifugin, cimifugin glycoside; 2. Coumarins composition: bergapten, psoralen, imperatorin, phellopterin, Dare appropriate because of, xanthotoxin, anomalin, scopoletin; 3. polyacetylene class becomes to send out: Panaxynol, falcarindiol, Radix Saposhnikoviae acidic polysaccharose A, C; 4. volatile oil is containing several 10 kinds of compositions, and totally 20 kinds that can identify, what content was higher has octanal, β-bisabolence, aldehyde C-9, and 7-octene-4-alcohol, aldehyde, alkene and sagittol etc. are analysed in side.5. also containing cupreol, β-paddy alcohol-β-D-Glucose glycoside, mannitol, vanillic acid etc.

Radix Saposhnikoviae, pungent, sweet, tepor.Return bladder, liver, spleen channel.There is expelling pathogenic wind from the body surface, removing dampness to relieve pain, effect of relieving convulsion.For headache due to common cold, rheumatic arthralgia, rubella pruritus, tetanus.

Modern pharmacological research shows, Radix Saposhnikoviae mainly contains the pharmacological action of following four aspects: 1. antiallergic, antiinflammatory: Radix Saposhnikoviae has the effect of the delayed hypersensitivity suppressed caused by DNCB; 2. antipyretic, analgesia: rabbit triple vaccine (pertussis, diphtheria, tetanus vaccine) is made animal pyrogenicity model.Radix Saposhnikoviae decocting liquid 2 g/kg, compares with anodynine and normal saline.Radix Saposhnikoviae decocting liquid refrigeration function in 1 ~ 2 hour is obvious; 3. convulsion: Radix Saposhnikoviae water extract can extend the incubation period that mice convulsion occurs; 4. other: its water decoction staphylococcus glucose coccus, beta hemolytic streptococcus and Diplococcus pneumoniae all have certain inhibitory action.

In conjunction with the characteristic that the effective ingredient of Radix Saposhnikoviae, pharmacological research, clinical practice and function cure mainly, Radix Saposhnikoviae adopts the preparation technology of water boiling and extraction in the present invention.

herba Asari(aristolochiaceae plant Herba Asari asarum heterotropoidesfr. Schmidt var. mandshuricum(Maxim.) Kitag., Seoul Herba Asari asarum sieboldiimiq. var. seoulensenakai or magnificent Herba Asari asarum sieboLdiimiq. dry root welding technology): Herba Asari herb is containing volatile oil 2.5%, in oil, main component is methyleugenol, safrole, asaricin, elemicin, still containing eucarvone, chavicol methylether, pinene, camphene, eucalyptol, demethyl coclaurine, pungent taste material pellitorine and-N-isobutyl group-2, 4, 8, 10-12 carbon tetraene amide etc., in oil, main component is α-thujene (α-myrcene, γ-terpineol, elemicin, still containing alpha-terpineol, safrole, methyleugenol, myristicin, linalool, asaricin, limonene etc. magnificent Herba Asari is also containing Pentadecane, sitosterol, campesterol, stigmasterol, sesamin and N-isobutyl group 14 carbon tetraene amide etc. Seoul Herba Asari is containing volatile oil 1%, in oil, main component is eucarvone, another containing α-or nopinene, eucalyptol, methyleugenol, safrole, α-methoxyl group safrole, elemicin, asaricin etc.

Herba Asari, pungent, temperature.GUIXIN, lung, kidney channel.There is expelling wind and cold, wind-expelling pain-stopping, sensible, effect of warming the lung to resolve fluid-retention.For anemofrigid cold, headache, toothache, nasal obstruction watery nasal discharge, allergic rhinitis, nasal sinusitis, rheumatic arthralgia, phlegm retention cough with asthma.

Modern pharmacological research shows, Herba Asari mainly contains the pharmacological action of following five aspects: 1. antipyretic effect; 2. on the impact of cardiovascular system: Herba Asari volatile oil obviously can increase the coronary flow of mice isolated heart; And energy blood pressure lowering, and decoct can boost; 3. to the effect of respiratory system: Herba Asari volatile oil has the relexation of highly significant to the tracheal smooth muscle that histamine or acetylcholine cause convulsion, and its antihistamine effect comparatively anti-acetylcholine is strong; 3. antiinflammatory; 4. other: antitussive, to eliminate the phlegm.

In conjunction with the characteristic that the effective ingredient of Herba Asari, pharmacological research, clinical practice and function cure mainly, Herba Asari adopts the preparation technology of water boiling and extraction in the present invention.

radix Glycyrrhizae(glycyrrhizic legume glycyrrhiza uralensisfisch., Glycyrrhiza inflata Bat. glycy-rrhiza inflataor Glycyrrhiza glabra L. Bat. glycyrrhiza glabral. dry root welding technology) Radix Glycyrrhizae contains number of chemical composition, and main component has glycyrrhizic acid, liquirtin etc.The chemical composition of Radix Glycyrrhizae is very complicated; from Radix Glycyrrhizae, isolated compound has the tens of kinds of compounds such as glycyrrhizin, liquiritin, isoliquiritin, neoliquriitin, neoisoliquiritin, glycyrrhizin, isoliquiritigenin and licoricidin (+)-Licoricidin., glycyrol, isoglycyrol, 7-methylcoumarin essence, umbelliferone so far, but these compositions and quantity usually can be different with the difference of the factors such as the kind of Radix Glycyrrhizae, planting area, collecting time.Large quantifier elimination shows, glycyrrhizin and Flavonoid substances are most important biological active substanceies in Radix Glycyrrhizae, are mainly present in the part within licorice epidermis.

Radix Glycyrrhizae, sweet, flat.GUIXIN, lung, spleen, stomach warp.There is spleen reinforcing stomach function regulating, effect of Yiqi and vein recovery.For weakness of the spleen and stomach, fatigue and weakness, severe palpitation, irregularly intermittent and regularly intermittent pulse.

Modern pharmacological research shows, Radix Glycyrrhizae mainly contains the pharmacological action of following four aspects: 1. to the effect of digestive system: in the removing extractum of glycyrrhizin and Radix Glycyrrhizae, flavonoid glycosides has obvious protective effect to Rat Experimental ulcer; 2. adrenocortical hormone sample effect: Radix Glycyrrhizae extractum, glycyrrhizin and enoxolone have promotion sodium to Healthy People and animal, the effect of water retention; Low dose of glycyrrhizin (every only 100 μ g) can make rat chest gland atrophy and adrenal gland's weight increase, and produces the effect of glucocorticoid cortisone sample.Time heavy dose of, then the effect of glucocorticoid sample is not obvious, only presents the effect of mineralocorticoid sample; 3. antiinflammatory: have obvious inhibitory action to Oleum Tiglii induced mice auricle edema; 4. other: antibacterial, antitumor, removing toxic substances etc.

In conjunction with the characteristic that the effective ingredient of Radix Glycyrrhizae, pharmacological research, clinical practice and function cure mainly, Radix Glycyrrhizae adopts the preparation technology of water boiling and extraction in the present invention.

Technique effect of the present invention is verified below by a test example:

1. test objective:

By design that is random, open, contrast, clinical observation is carried out to the curative effect of the kidney yin and yang deficiency type of all cards before and after treatment postmenopausal women of the present invention syndromic combinational drug therapy postmenopausal women and safety.

subjects inclusive criteria:

1. Western medicine diagnose standard is met.

2. tcm diagnosis standard is met.

3. the age was the women of 40 ~ 55 years old

4. hormone and other treatment by Chinese herbs person was not used in 3 months.

5. the patient of Informed Consent Form has been signed.

6. can the person of following up a case by regular visits to.

subjects exclusion standard:

1. patient does not meet the diagnostic criteria of doctor trained in Western medicine and the CM syndrome differentiation criterion of the traditional Chinese medical science.

2. patient age is below 40 years old or more than 55 years old.

3. in 3 months, used gonadal hormone or used the influential medicine of sex hormone level.

4. the irregular colporrhagia of unknown cause is not cured, and hysterectomy is postoperative, after bilateral oophorectomy, and gynecological's organic disease and genital malignant tumor person.

5. the organ diseases such as severe cardiac cerebrovascular, liver, kidney and endocrine metabolism disease, hypertension, psychotic is merged.

6. the patient not signing Informed Consent Form and can not follow up a case by regular visits to.

safety observation index:

1. blood, urine, stool routine examination inspection.

2. Liver and kidney function inspection.

health giving quality observation index:

(1) cardinal symptom: coldness of the body with chills, Tidal fever with perspiration, dim complexion, emotional, insomnia, tired, nocturia frequency, Musculoskeletal pain, puffiness of the face and limbs, dizzy, the symptoms such as cardiopalmus, depression, sexual anhedonia.

(2) main physical signs: picture of the tongue, pulse condition etc.

(3) physico-chemical examination: blood FSH .LH .E2 and blood, urine, feces routine examination, first 3 must be done, and other need choosing as one sees fit to do according to the state of an illness.

object of study is divided into groups:

Selected meet above-mentioned standard and the menopausal syndrome patient of this clinical observation of voluntary participation amounts to 130 examples, be divided into treatment group and matched group by random packet principle.Treatment group: 100 example, age 40-55 year, average 50.33 ± 3.11 years old, course of disease 4-7, average 6.14 ± 1.70 years; Matched group: 30 example, age 40-55 year, average 50.00 ± 2.841 years old, course of disease 4-7, average 5.97 ± 1.73 years; Two groups of physical data have comparability.

therapeutic Method:

Treatment group: by prescription of the present invention from intending Gengnian Decoction: Radix Rehmanniae Preparata 12g RHIIZOMA DIOSCOREAE fr0m Henan of China 12g Fructus Corni 12g Rhizoma Curculiginis 12g Herba Epimedii 12g Radix Morindae Officinalis 12g Radix Angelicae Sinensis 10g Ramulus Cinnamomi 9g Radix Angelicae Pubescentis 9g Radix Saposhnikoviae 9g Herba Taxilli 12g Herba Asari 3g Radix Glycyrrhizae Preparata 6g.Every agent makes by No. 2 Affiliated Hospital of Guiyang College of Traditional Chinese Medicine's machine room of boiling medicine 3 bags, the packed Chinese medicine that specification is 100ml/ bag.And add and subtract with disease.

Matched group: by teaching material side Radix Rehmanniae Preparata 15g, Radix Aconiti Lateralis Preparata (gun tab) 6g, Cortex Cinnamomi 6g, Rhizoma Dioscoreae 12g, Fructus Corni 12g, Semen Cuscutae 12g, Colla cornus cervi 6g, Fructus Lycii 15g, Radix Angelicae Sinensis 10g, Cortex Eucommiae 10g, every agent makes by No. 2 Affiliated Hospital of Guiyang College of Traditional Chinese Medicine's machine room of boiling medicine 3 bags, the packed Chinese medicine that specification is 100ml/ bag.And add and subtract with disease.

the course for the treatment of:

Treatment group: by prescription of the present invention from the Gengnian Decoction 100ml intended, oral, 3 times/day; Matched group: teaching material side 100ml, oral, 3 times/day.Two groups was all 1 course for the treatment of with 30 days, observed treatment 3 course for the treatment of.Two groups, while taking medicine, are all carried out the health education of menopause and post menopausal diseases to patient and psychology is nursed.

curative effect determinate standard:

Cure: tcm clinical practice symptom, sign disappear or substantially disappear, syndrome integral Jian Shao≤95%

Effective: tcm clinical practice symptom, sign are obviously improved, syndrome integral Jian Shao≤70%;

Effective: tcm clinical practice symptom, sign all take a favorable turn, syndrome integral Jian Shao≤30%;

Invalid: tcm clinical practice symptom, sign are all not improved, even increase the weight of, syndrome integral reduces less than 30%.

All clinical data data are by SPSS 16.0 statistical analysis.Measurement data represents with " x ± s ", compares employing independent samples t-test between group, compares employing one factor analysis of variance in group between each course for the treatment of; Enumeration data covariance analysis; Curative effect ranked data adopt rank test.

result of the test is as shown in following table 1-table 6:

Note: 1, before mutual item group * medication: F=0.229, P=0.633 > 0.05, can think that each group of regression straight line is parallel, can covariance analysis be used; The F=5.113 of 2.Corrected Model, P=0.007 < 0.05, selected model has statistical significance; 3. the statistic of test F=6.700 of group, P=0.011 < 0.05, after the FSH before correcting medication is described, the FSH difference after two groups of treatments has statistical significance; 4. F=4.324, P=0.040 < 0.05 before medication, can think that the FSH before covariant medication has statistical significance to the impact after medication; 5. the mean matched group 67.755 > treatment group 63.103 after adjustment, so treatment group is better than matched group.

Note: 1, before mutual item group * medication: F=2.236, P=0.137 > 0.05, can think that each group of regression straight line is parallel, can covariance analysis be used; The F=37.783 of 2.Corrected Model, P=0.000 < 0.05, selected model has statistical significance; 3. the statistic of test F=39.642 of group, P=0.000 < 0.05, after the LH before correcting medication is described, the LH difference after two groups of treatments has statistical significance; 4. F=44.153, P=0.000 < 0.05 before medication, can think that the LH before covariant medication has statistical significance to the impact after medication; 5. the mean matched group 44.436 > treatment group 39.882 after adjustment, so treatment group is better than matched group.

Note: 1, before mutual item group * medication: F=0.716, P=0.399 > 0.05, can think that each group of regression straight line is parallel, can covariance analysis be used; The F=99.842 of 2.Corrected Model, P=0.000 < 0.05, selected model has statistical significance; 3. the statistic of test F=44.340 of group, P=0.000 < 0.05, after the E2 before correcting medication is described, the E2 difference after two groups of treatments has statistical significance; 4. F=58.437, P=0.000 < 0.05 before medication, can think that the E2 before covariant medication has statistical significance to the impact after medication; 5. the mean matched group 82.657 < treatment group 92.848 after adjustment, so treatment group is better than matched group.

Note: treat two groups of comparing difference not statistically significants (P > 0.05) after 1 course for the treatment of for two groups; Two groups treat 2 courses for the treatment of after two groups of comparing differences have statistical significance (P < 0.05); Two groups treat 3 courses for the treatment of after two groups of comparing differences have remarkable statistical significance (P < 0.01), treatment group comparatively matched group improves symptom better efficacy.

safety analysis:

During all cases Ureteral Calculus, monitoring Liver and kidney merit, three large routines etc. are all unchanged closely.Have in treatment group an example start to take medicine a few days ago in, occur diarrhoea, but symptom is slight, every day 2-3 time, do not affect continual cure, symptom takes a turn for the better gradually two days later, and rear disappearance in a week, adverse reaction rate is 1%.Have an example to occur in matched group dizzy, there is weak, drowsiness, xerostomia in two examples, but symptom is slight, and do not affect continual cure, adverse reaction rate is 10%.Consider from drug safety: treatment group is relatively better.

conclusion and discussion:

Found by the clinical observation of above two groups of cases research, treatment group for the primary symptom of kidney yin deficiency of yang type patient and the treatment of secondary disease and in reduction serum follotropin, lutropin, raising serum estradiol treatment group be all obviously better than matched group.Based on above result, the present invention and Yougui Wan, the kidney-Yang-Reinforcing Bolus compare and are analyzed as follows by we: certainly intend Gengnian Decoction, composition: Radix Rehmanniae Preparata 12g RHIIZOMA DIOSCOREAE fr0m Henan of China 12g Fructus Corni 12g Rhizoma Curculiginis 12g Herba Epimedii 12g Radix Morindae Officinalis 12g Radix Angelicae Sinensis 10g Ramulus Cinnamomi 9g Radix Angelicae Pubescentis 9g Radix Saposhnikoviae 9g Herba Taxilli 12g Herba Asari 3g Radix Glycyrrhizae Preparata 6g.Aversion to cold and cold limbs person Ramulus Cinnamomi is easily Cortex Cinnamomi 10g, clear urine in large amounts, and nocturia frequency person adds Fructus Schisandrae Chinensis 12g, Herba Cistanches 12g, Ootheca Mantidis 12g, and kidney tonifying is astringent or styptic treatment for spontaneous sweating, and kidney deficiency in both YIN and YANG adds CARAPAX ET PIASTRUM TESTUDINIS PREPARATA 15g, Fructus Ligustri Lucidi 12g, Herba Ecliptae 12g, and the obvious person of kidney yang virtual loss adds Cornu Cervi Degelatinatum 15g.Radix Rehmanniae Preparata, RHIIZOMA DIOSCOREAE fr0m Henan of China, Fructus Corni nourishing kidney yin in side, with treating YANG within YIN; Rhizoma Curculiginis, Herba Epimedii, Radix Morindae Officinalis warming and recuperating the kidney-YANG; Ramulus Cinnamomi, Radix Angelicae Sinensis strengthen promoting the flow of QI-blood by warming the meridian, the merit of supporing yang activating QI; And Ramulus Cinnamomi can enter Liver and kidney blood system and cold relieving; Radix Angelicae Pubescentis, Herba Asari enter kidney channel, can search volt wind; Radix Saposhnikoviae all row flesh table, makes it to go out; Herba Taxilli expelling wind and dampness, to reach outside aid yang qi.Full side plays the kidney invigorating warming YANG altogether, the merit of expelling wind and cold.Yougui Wan, the kidney-Yang-Reinforcing Bolus: Radix Rehmanniae Preparata 15g, Radix Aconiti Lateralis Preparata (gun tab) 6g, Cortex Cinnamomi 6g, Rhizoma Dioscoreae 12g, Fructus Corni 12g, Semen Cuscutae 12g, Colla cornus cervi 6g, Fructus Lycii 15g, Radix Angelicae Sinensis 10g, Cortex Eucommiae 10g.If hold concurrently menorrhagia or metrorrhagia and metrostaxis person, reuse Colla cornus cervi, add with Halloysitum Rubrum, Fructus Psoraleae, to strengthen the merit that the kidney warming Gu Chong only collapses, if hold concurrently the obvious person of back cold type of pain, add Fructus Zanthoxyli, Radix Morindae Officinalis, to augment kidney invigorating YANG, the effect of temperature compensation governor vessel.In side with Radix Aconiti Lateralis Preparata, Cortex Cinnamomi, Colla cornus cervi for monarch drug, warming and recuperating the kidney-YANG, benefiting essence and marrow.Minister with Radix Rehmanniae Preparata, Fructus Lycii, Fructus Corni, Rhizoma Dioscoreae nourishing YIN for benefiting the kidney, nourishing the liver spleen reinforcing.Help with Semen Cuscutae YANG invigorating tonifying YIN, controlling nocturnal emission with astringent drugs reducing urination; Cortex Eucommiae liver and kidney tonifying, bone and muscle strengthening; Chinese angelica blood supplementing nourishing the liver.All medicines coordinate, and play warming and recuperating the kidney-YANG altogether, and replenishing essence stops the merit of something lost.This side comparatively front compares the product without expelling wind and cold, therefore promoting the flow of QI-blood by warming the meridian, the outer soothing the liver power of aid yang qi is unequal to front.This is two side's prescriptions, the adopted difference in side.Namely be the mechanism of action place being better than Yougui Wan, the kidney-Yang-Reinforcing Bolus from plan Gengnian Decoction.From its modern pharmacology mechanism of action, prescription of the present invention has the kidney invigorating warming YANG YIN nourishing, increases immunologic function, improves microcirculatory effect certainly.Reproductive endocrine-the immunoloregulation function of patient recovers to reach new adjustment from disorder and symptom is improved.And Radix Angelicae Pubescentis, Radix Saposhnikoviae, Herba Taxilli, Herba Asari have blood vessel dilating, spasmolytic diuresis, improve the effect of microcirculation, whether it can improve or strengthen pelvic cavity blood circulation, thus promote the increase of ovary blood volume, impel ovarian secretion hormone amount to increase, need further research and exploitation.During all cases Ureteral Calculus, closely monitoring Liver and kidney merit, the hematuria just lab testing such as conventional, have no significant change, clinical practice has safety.Therefore before and after treatment postmenopausal women of the present invention syndromic combinational drug therapy postmenopausal women, the kidney yin and yang deficiency type health giving quality of all cards is good, safety is high, be a kind ofly treat the Chinese medicine preparation that before and after postmenopausal women, all cards are effective, easy, safe.

model case:

Ni, female, 48 years old, because " lumbosacral aching pain companion palpitation and insomnia 1 year, increases the weight of January repeatedly " went to a doctor on October 12nd, 2007.Patient is symptom partial rcsponse after taking climacteric Drug therapy.There is coldness of the body with chills, lumbosacral aching pain, clear urine in large amounts, nocturia frequency without obvious inducement afterwards, before March, withdraw exacerbation of symptoms after medicine voluntarily.Existing disease is seen: coldness of the body with chills, lumbosacral aching pain, clear urine in large amounts, nocturia frequency, dim complexion, lethargy, palpitation and insomnia, vertigo and tinnitus, forgetful, light red tongue, white, slippery and moist fur, deep-thready pulse.Tcm diagnosis is " menopausal syndrome ", and card belongs to insufficiency of kidney-YANG, and control suitable the kidney invigorating warming YANG, expelling wind and cold, gives the syndromic combinational drug therapy for the treatment of postmenopausal women of the present invention, oral each 10g, every day 3 times.Have rosy cheeks during further consultation after January, spirit can, appetite comparatively before take a turn for the better, tongue arteries and veins is substantially the same.Prompting treatment effectively, continues to take the syndromic drug regimen for the treatment of postmenopausal women of the present invention, and tell coldness of the body with chills, lumbosacral aching pain, vertigo and tinnitus disappearance after February during further consultation, sleep obviously improves, and only slightly feels lumbosacral aching pain after fatigue, reaches clinical cure.

Accompanying drawing explanation

Fig. 1 is preparation technology's flow chart;

Fig. 2 is decocting process reference substance chromatogram;

Fig. 3 is decocting process orthogonal experiment chromatogram one;

Fig. 4 is decocting process orthogonal experiment chromatogram two;

Fig. 5 is decocting process orthogonal experiment chromatogram three;

Fig. 6 is water extraction process three batches checking chromatogram;

Fig. 7 is that pilot scale sample size measures chromatogram;

Fig. 8 is middle test agent TLC distinguish collection of illustrative plates.

Detailed description of the invention

Below in conjunction with drawings and Examples, the present invention is described in further detail, but protection scope of the present invention is not by any restriction of specific embodiment, but be limited by claim.

Embodiment:

one. prescription and method for making:

Prescription: Radix Rehmanniae Preparata 120 g, Rhizoma Curculiginis 120 g, Rhizoma Dioscoreae 120 g, Fructus Corni 120 g, Herba Epimedii 120 g, stir-baking RADIX MORINDAE after sprinking salt solution sky 120 g, Herba Taxilli 120 g, wine Fructus Ligustri Lucidi 120 g, Herba Ecliptae 120 g, Radix Angelicae Sinensis 100 g, Ramulus Cinnamomi 90 g, Radix Angelicae Pubescentis 90 g, Radix Saposhnikoviae 90 g, Herba Asari 30 g, Radix Glycyrrhizae 60 g; Make 1000 mL.

Method for making: above ten five kinds of Chinese medicine, decoct with water 3 times, first time adds 10.5 times of water, and second and third time respectively adds 8 times of water gagings, and each decoction 0.5 hour, filters, merging filtrate; Filtrate normal pressure is concentrated to about 800 mL, adds 0.5 g ethyl hydroxybenzoate (using a small amount of dissolve with ethanol), and mixing, adds purified water to 1000 mL, stir evenly, subpackage, to obtain final product.

Preparation technology's flow chart of the present invention as shown in Figure 1.Process route of the present invention is determined as follows: ten gomi herbs such as Radix Rehmanniae Preparata, Rhizoma Curculiginis, Rhizoma Dioscoreae decoct with water extraction → Aqueous extracts simmer down to medicinal liquid → add appropriate ethyl hydroxybenzoate, mixing, standardize solution, subpackage → finished product.

two. medical material pre-treating technology is studied:

radix Rehmanniae Preparata:scrophulariaceae rehmannia glutinosa plant rehmannia glutinosathe Preparation process product of dried root Libosch., cut sheet or block, dry, to obtain final product.

rhizoma Curculiginis:amrallid Rhizoma Curculiginis curculigo orchioidesgaertn. dry rhizome, removing root head and fibrous root, cleaning, drying.

rhizoma Dioscoreae:dioscoreaceae plant Rhizoma Dioscoreae dioscorea oppositathunb. dry rhizome, removing impurity, separately size, bubble profit, to thoroughly, cuts sheet, dry.

fructus Corni:cornaceae plant Fructus Corni cornus officinalissieb. the drying and ripening sarcocarp of et Zucc..

herba Epimedii:berberidaceae plant Herba Epimedii epimedium brevicornumaxim., Epimedium sagittatum epimedium sagittatum(Sieb. et Zucc.) Maxim., E. Pubescens epimedium pubescensor Herba Epimedii Maxim. epimedium koreanumthe dried leaves of Nakai.

stir-baking RADIX MORINDAE after sprinking salt solution sky:maguireothamnus speciosus Radix Morindae Officinalis morinda officinalisthe dry root of How, steams method (" Chinese Pharmacopoeia " annex II D in 2010) according to salt and steams thoroughly, remove core while hot, cutting, dry.

herba Taxilli:loranthaceae plant Herba Taxilli taxillus chinensis(DC.) the dry stem and branch with leaf of Danser, removes thick stem, cutting, dry, or dry after steaming.

wine Fructus Ligustri Lucidi:oleaceae plants Fructus Ligustri Lucidi ligustrum lucidumait. dry mature fruit, steams method (" Chinese Pharmacopoeia " annex II D in 2010) according to stewed with wine method or wine and stewes to wine absorption or steam thoroughly.

herba Ecliptae: feverfew Eclipta prostrata ecLipta prostratal. dry aerial parts, removing impurity, slightly washes, cutting, dry.

radix Angelicae Sinensis:umbelliferae angelica angelica sinensis(OLiv.) dry root of Diels, removing impurity, cleans, runs through, shave, dry or cold drying.

ramulus Cinnamomi: canella Cortex Cinnamomi cinnamomum cassiathe dry twig of Presl, removing impurity, cleans, runs through, cut sheet, dry.

radix Angelicae Pubescentis: samphire Angelica pubescens angelica pubescensmaxim. f. biserratathe dry root of Shan et Yuan, removing impurity, cleans, runs through, shave, dry or cold drying.

radix Saposhnikoviae: umbelliferae Saposhnikovia divaricata saposhnikovia divaricata(Turcz.) dry root of Schischk., removing impurity, cleans, runs through, cut sheet, dry.

herba Asari: aristolochiaceae plant Herba Asari asarum heterotropoidesfr. Schmidt var. mandshuricum(Maxim.) Kitag., Seoul Herba Asari asarum sieboldiimiq. var. seoulensenakai or magnificent Herba Asari asarum sieboldiimiq. dry root welding technology, removing impurity, spray clear water, slightly moistens, cutting, dries in the shade.

radix Glycyrrhizae: glycyrrhizic legume glycyrrhiza uralensisfisch., Glycyrrhiza inflata Bat. glycy-

rrhiza inflataor Glycyrrhiza glabra L. Bat. glycyrrhiza glabral. dry root welding technology, removing impurity, cleans, runs through, cut sheet, dry.

three. the screening of process conditions:

(1) Study on extraction:

the mensuration of 1 medical material water absorption rate:

Take 1/2 clinical prescription amount medical material and amount to 77 g, add 8 times amount water soakings, soak different time, filter, claim wet medicine weight (to medical material weight no longer increases), measure medical material water absorption rate, formula following (the results are shown in Table 1-1).

Quality (g) under mb-medical material water suction saturation

Mg-medical material quality (g) in the dry state

From table 1-1, medical material water absorption rate is 2.1 times, for convenience of operation, is 2.5 times of calculating with water absorption rate, and namely medical material water absorption is heavy 2.5 times of medical material.Therefore first water extraction time should add the water of 2.5 times amount of medical material weight.

the single factor exploration of soak time:

Under the condition meeting medical material water absorption rate, investigate soak time, on the impact of prescription paste-forming rate, with total solid matters yield for inspection target, optimize the soak time that prescription paste-forming rate is had the greatest impact.

test method and result:

2.1.1 sample preparation:

Take 1/2 clinical prescription amount medical material and amount to 77 g, add 10.5 times of water gagings first, add 8 times of water gagings for 2 times afterwards, be accustomed to according to traditional medication and consider Preparation Room in Hospital practical condition, soaking 0 h, 0.5 h, 4 h and soaked overnight respectively, decoct 2 times, each 0.5 h, filters, merging filtrate, medicinal liquid is dissolved to 50 mL, for subsequent use.

total solid matters yield measures:

From above-mentioned medicinal liquid, each precision gets two part of 10 mL, in the evaporating dish of constant weight, and water bath method, measure according to dry weightless mensuration (" Chinese Pharmacopoeia " version in 2010 annex IX G), calculate total solid matters yield, formula following (the results are shown in Table 2-1, variance analysis is in Table 2-2).

W is the weight of solids in 10 mL filtrates, and V is constant volume, and Wt is sample charged material weight.

One factor analysis of variance shows: p>0.05, illustrates different soak time there was no significant differences; According to traditional handicraft and cost-saving needs, soak time is selected to be 0.5 h.

the quadrature factor of decocting process:

Under the condition meeting medical material water absorption rate, soak 0.5 h, investigate the principal element of amount of water, decocting time, decoction number of times three impact decoction effect, by each factor 3 level, use L ₉(3 ⁴) orthogonal table arrangement test (table 3-1).Boiled process adopts orthogonal test to carry out screening (table 3-2), fully curative effect of medication can be reflected in order to make technique, and cure mainly in conjunction with the our feature of medicine composition and the function of we, with the content of the anti-icariin of the effective ingredient of Herba Epimedii in we for index is evaluated technique, preferred abstract methods.Orthogonal result variance analysis and factor level analysis in table 3-3, table 3-4.

sample preparation:

Take 1/2 clinical prescription amount medical material and amount to 77 g, (first time soaks 0.5 h), and medicinal liquid filters, merging filtrate, and concentrated, medicinal liquid is settled to 50 mL, for subsequent use to be undertaken testing (table 3-2) by orthogonal test table.

the mensuration of icariin in test specimen:

3.2.1 instrument:

AgiLent technoLongies 1260 Infinity, G4212B DAD diode array detector; Prunus mume (sieb.) sieb.et zucc. Teller MS105DU electronic balance; Shu Mei KQ-500DA type numerical control ultrasonic cleaner.

reagent:

Acetonitrile (chromatographically pure), Diluted Alcohol, methanol (analytical pure), water is redistilled water, icariin reference substance (National Institute for Food and Drugs Control, national drug standards material, lot number: 110737-200415), treatment postmenopausal women of the present invention syndromic drug regimen orthogonal test sample, lacks the negative sample of Herba Epimedii.

method and result:

3.3.1 chromatographic condition and system suitability test:

With the chromatographic column Agilengt C that octadecylsilane chemically bonded silica is filler ₁₈(CAPCELL PAKC18,5 μm, 4.6 × 250 mm); Acetonitrile-aqueous solution (30:70) is mobile phase; Determined wavelength is 270nm.Number of theoretical plate calculates should be not less than 3000 by icariin peak.

the preparation of reference substance storing solution:

Precision takes puts in 20 mL measuring bottles at 60 DEG C of drying under reduced pressure icariin reference substance 0.02028 g of 4 hours, adds methanol ultrasonic dissolution and to scale, shakes up, being made into the storing solution of 1.014 mg/mL.

3.3.3 the preparation of reference substance solution:

Precision measures reference substance storing solution 1.0 mL and puts in 10 mL measuring bottles, adds methanol to scale, shakes up, and makes the reference substance solution of 0.1014 mg/mL; Reference substance chromatogram as shown in Figure 2.

need testing solution is prepared:

Precision measures sample 2 mL and puts in 20 mL measuring bottles, adds methanol dilution to scale, shakes up, and filter, subsequent filtrate is as need testing solution.

3.3.5 content measurement:

Accurate absorption reference substance solution and each 10 μ L of need testing solution, injection liquid chromatography, measures, record, calculates content, the results are shown in Table 3-2, table 3-3, Fig. 3, Fig. 4, Fig. 5.

interpretation of result:

With Icariin content in sample for index, from table 3-2, table 3-3 analyze can obtain: factor A, B, C there are no significant impact, for secondary cause, in conjunction with the production decision of clinical prolonged application, consider that cost-saving and need of production selects A level 1, B level 3, C level 1, then this prescription is drafted optimal extract process and is: A ₁b ₃c ₁, namely prescription medical material decocts with water 3 times, and first time adds 10.5 times of water, and second and third time respectively adds 8 times of water gagings, and each decoction 0.5 hour, filters, merging filtrate.

the process certification experiment of three batch samples:

Get prescription medical material, by optimal extract process A ₁b ₃c ₁extract, measure Icariin content, result shows (see table 3-5, Fig. 6): total solid matters yield meansigma methods is 31.88%, RSD=2.87%, illustrates that process stabilizing is feasible.

concentrated:

because said preparation is traditional handicraft mixture, consider traditional habit and practical condition, adopt normal pressure to concentrate.

(2) preparations shaping technical study:

In order to increase the stability of mixture, ensureing the quality of preparation, effective antiseptic must be selected.According to " Chinese Pharmacopoeia " version in 2010, medicinal the most frequently used antiseptic is respectively benzoic acid and sodium benzoate, sorbic acid and potassium salt thereof and ethyl hydroxybenzoate class.Benzoic acid and sodium benzoate is a kind of acid antiseptic, and when pH is 2.5 ~ 4, its bacteriostasis is stronger; As pH>5.5, fungistatic effect obviously weakens, and poor to fungi and yeasts inhibition, and toxicity is larger.Therefore, should not as the antiseptic of this preparation.Sorbic acid and potassium salt thereof are also acid antiseptic, have fine inhibitory action to fungi and yeasts, and its pH value range be suitable for is <6.The toxicity ratio benzoic acid and sodium benzoate of ethyl hydroxybenzoate is much lower, and application pH value range is wide, and its bacteriostasis by the impact of pH value, has antibacterial action widely to mycete, yeast, antibacterial hardly.

the selection of antiseptic:

Get sorbic acid (" Chinese Pharmacopoeia " 2010 editions containing 0.3% respectively, specify maximum sorbic acid consumption) and 0.05% ethyl hydroxybenzoate (" Chinese Pharmacopoeia " 2010 editions, specify maximum sorbic acid consumption) the syndromic drug regimen for the treatment of postmenopausal women of the present invention be 45 DEG C in temperature, respectively 0 month, January, February, March, relative humidity is under 75% environment, measure according to microbial limit method (" Chinese Pharmacopoeia " version in 2010 annex XIII C) and check, the results are shown in Table 5-1.

Result: sorbic acid starts anticorrosion inefficacy for 3 months under acceleration conditions.

Get the syndromic drug regimen for the treatment of postmenopausal women of the present invention containing the sorbic acid of 0.3% and the ethyl hydroxybenzoate of 0.05% respectively respectively at 0 month, 3 months, 6 months, 7 months, under room temperature environment, measure according to microbial limit method (" Chinese Pharmacopoeia " version in 2010 annex XIII C) and check, the results are shown in Table 5-2.

Result: sorbic acid at ambient temperature 6 months time start anticorrosion inefficacy.

According to table 5-1, table 5-2 drafts the antiseptic selecting ethyl hydroxybenzoate as the syndromic drug regimen for the treatment of postmenopausal women of the present invention.

the consumption of antiseptic:

Heavier according to this preparation moisture content, the feature of easy moldy metamorphism, and " Chinese Pharmacopoeia " (version one in 2010) regulation, the consumption of oxybenzene esters must not more than 0.05%, therefore to select concentration to be respectively 0.01%, 0.03%, 0.05% ethyl hydroxybenzoate be that the antiseptic of this preparation contrasts.

Get the syndromic drug regimen for the treatment of postmenopausal women of the present invention (respectively containing ethyl hydroxybenzoate 0.01%, 0.03%, 0.05%) containing variable concentrations ethyl hydroxybenzoate, respectively at 0 month, 1 month, 2 months, 3 months, it is 45 DEG C in temperature, relative humidity is under 75% environment, measure according to microbial limit method (" Chinese Pharmacopoeia " version in 2010 annex XIII C) and check, the results are shown in Table 5-3.

Result: 0.01% ethyl hydroxybenzoate, 0.03% ethyl hydroxybenzoate under acceleration conditions 3 months time anticorrosion inefficacy.

Get the syndromic drug regimen for the treatment of postmenopausal women of the present invention (respectively containing ethyl hydroxybenzoate 0.01%, 0.03%, 0.05%) containing variable concentrations ethyl hydroxybenzoate, respectively at 0 month, 3 months, 6 months, 7 months, under room temperature environment, measure according to microbial limit method (" Chinese Pharmacopoeia " version in 2010 annex XIII C) and check, the results are shown in Table 5-4.

Result: 0.01% ethyl hydroxybenzoate, 0.03% ethyl hydroxybenzoate at ambient temperature 7 months time anticorrosion inefficacy.

By the accelerated tests of 3 months and 7 months stability tests, find only have the ethyl hydroxybenzoate of 0.05% to have good antisepsis, the quality of preparation can be guaranteed, therefore select 0.05% concentration as antiseptic.

(3) pilot scale research:

Carrying out three batches of amplifications, 10 times of productions by working out preparation process condition, comprehensive assessment being carried out to production technology index, performance rating is carried out to medical material and finished product, investigating the reasonability of this product preparation technology.Shown by data in pilot process parametric statistics table, 100 bottles of (100 mL/ bottle) finished products should be produced by fed intake theory, work out said preparation finished product net yield and be not less than 85%.

Show through three batches of scale up test results, the every technical parameter of this product is identical with technique optimum condition, and stable process conditions, feasible process is described, is applicable to batch production (see table 6-1, Fig. 7, Fig. 8).

Certainly, the embody rule example of more than just invention, the technical scheme that the present invention also has other embodiment, all employings to be equal to replacement or equivalent transformation to be formed, all drops within protection domain of the presently claimed invention.

Claims (1)

1. treat the syndromic drug regimen of postmenopausal women, it is characterized in that: it is made up of the crude drug of following parts by weight: Radix Rehmanniae Preparata 114 ~ 132 parts, Rhizoma Curculiginis 114 ~ 132 parts, Rhizoma Dioscoreae 114 ~ 132 parts, Fructus Corni 114 ~ 132 parts, Herba Epimedii 114 ~ 132 parts, 114 ~ 132 parts, stir-baking RADIX MORINDAE after sprinking salt solution sky, Herba Taxilli 114 ~ 132 parts, wine Fructus Ligustri Lucidi 114 ~ 132 parts, Herba Ecliptae 114 ~ 132 parts, Radix Angelicae Sinensis 95 ~ 110 parts, Ramulus Cinnamomi 94.5 ~ 99 parts, Radix Angelicae Pubescentis 94.5 ~ 99 parts, Radix Saposhnikoviae 95 ~ 99 parts, Herba Asari 31.5 ~ 33 parts, 57 ~ 66 parts, Radix Glycyrrhizae.