CN104938789B - A kind of biological fermentation feed and preparation method thereof and special equipment - Google Patents
A kind of biological fermentation feed and preparation method thereof and special equipment Download PDFInfo
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- CN104938789B CN104938789B CN201510289424.9A CN201510289424A CN104938789B CN 104938789 B CN104938789 B CN 104938789B CN 201510289424 A CN201510289424 A CN 201510289424A CN 104938789 B CN104938789 B CN 104938789B
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- 230000004151 fermentation Effects 0.000 title claims abstract description 45
- 238000002360 preparation method Methods 0.000 title claims abstract description 9
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- 239000002994 raw material Substances 0.000 claims abstract description 32
- 238000002156 mixing Methods 0.000 claims abstract description 29
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- 238000000034 method Methods 0.000 claims abstract description 16
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- 101710089042 Demethyl-4-deoxygadusol synthase Proteins 0.000 claims abstract description 6
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- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims abstract description 6
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- 238000011081 inoculation Methods 0.000 claims abstract description 4
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- -1 compound vitamin Chemical class 0.000 claims description 6
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Landscapes
- Fodder In General (AREA)
Abstract
The present invention relates to a kind of biological fermentation feed and production technology and special equipment, production technology includes that raw material is just clear, crushes, mixing, inoculation, is packaged, fermentation, simple for process, easy to operation, it can be achieved that mechanization continuous production.It is to add premix using bio-matrixes such as cheap agricultural and sideline product leftover bits and pieces such as apple pomace, date powder, peanut shell, DDGS, whitewashing maize peel and bacterium solution is made through mixed fermentation that it, which is formulated,.The biological fermentation feed stable quality of preparation, palatability is good, nutritive value is comprehensive, viable bacteria content is high, preferably stores transport, can be used as livestock and poultry diet Direct-fed, and can substitute 20%~30% perfect compound feed, really realizes and saves food, reduces aquaculture cost.
Description
The present invention is application number 201310204789.8, the applying date：On May 29th, 2013, denomination of invention：A kind of " biology
The divisional application of fermented feed and preparation method thereof and special equipment ".
Technical field
The present invention relates to poultry poultry fodder, more particularly to a kind of biological fermentation feed and preparation method thereof and special equipment.
Background technology
Currently, that there is medicament residues is big, food security potential threat degree is big for feed and cultivation industry, feed profit is empty
Between it is low, aquaculture cost is high, market upheaval amplitude is big, and people and animals strive the particularly thorny of grain, and a large amount of agricultural and sideline product leftover bits and pieces resources do not have
It is reasonably developed and used etc. under the present situation of outstanding problems, utilizes plant haulm leather shell, agricultural byproduct ferment making section
The green safe biological feedstuff of grain nonreactive meets the grand premise of the Scientific Outlook on Development, meets national Industry Policy, can become give up into
Treasured reduces feed cost, increases culture efficiency, eliminates environmental pollution, reduces disease, and safe and secure food is provided for consumer
Product.
A lot of research work has been done in terms of biological fermentation feed production technology by some enterprises of the country and scientific research department at present,
And achieve gratifying progress and certain achievement.But mostly it is only limitted to fermented stalk, fermented bean dregs, fermentation cotton dregs, hair at present
The single feeds raw material such as ferment brewex's grains, even if there is the manufacturer of fermenting mixed raw material, production technology is still with traditional family
The workshop-based accumulated solids fermentation in front yard, batch based on batch production, not yet form mechanization, serialization, large-scale production.It is existing
Some fermented feeds often use fresh beer vinasse or fresh soy sauce grain etc. to be used as raw material, but the moisture of final products is difficult to control, and
And frequently encounter incomplete mixing, winter fermentation is difficult, strain survival rate is low, the degree of fermentation is difficult to control, easy microbiological contamination is gone mouldy, quality not
Stablize, technical bottlenecks and the product defects such as nutritive value is not high, palatability is poor, should not store.
Invention content
In view of the deficiencies in the prior art, it can realize that mechanization is continuously given birth to the purpose of the present invention is to provide a kind of
Quality is stable, palatability is good, nutritive value is comprehensive, viable bacteria content is high, preferably store transport a kind of biological fermentation feed and its
Preparation method and special equipment.
To achieve the above objectives, the technical solution adopted by the present invention is that：
A kind of biological fermentation feed, crude protein content reaches 30%-35%, crude fibre≤10%, cinder in the fermented feed
Point≤10%, calcium content 0.5%-1.2%, total phosphorus >=0.2%, sodium chloride content 0.2%-0.9%, moisture 40%-45%, viable count
Amount >=50 hundred million/gram.
Proteinase activity is 550-650U, cellulase activity 5900-6000U, phytic acid enzyme activity in the fermented feed
Property is 120-150U.
The fermented feed includes that the score ratio mixed fermentation by weight of following components forms：100 parts of DDGS, apple pomace 96
Part, 40 parts of date powder, 140 parts of peanut shell powder, 60 parts of maize peel of whitewashing, worm is 100 parts husky, 20 parts of premix, 30 parts of bacterium solution, molasses 6
Part, 408 parts of water；
The premix includes following components by weight：600 parts of mountain flour, 20 parts of medical stone, 20 parts of compound vitamin are compound
100 parts of micro- mine, 100 parts of calcium monohydrogen phosphate, 150 parts of salt, 5 parts of flavouring agent, 5 parts of sweetener；
The weight proportion of the wherein compound vitamin is as follows：
Vitamin A 5000KIU/kg vitamin Ds3 1000KIU/kg
Vitamin E 50000mg/kg vitamin Ks3 2500mg/kg
Vitamin B12000mg/kg vitamin Bs2 7500mg/kg
Vitamin B65000mg/kg vitamin Bs12 250mg/kg
Biotin 250mg/kg niacin 25000mg/kg
Calcium pantothenate 20000mg/kg folic acid 25mg/kg
Surplus is cornstarch；
The proportioning of compound micro- mine is as follows：
Copper 5g/kg；Iron 40g/kg；Manganese 30g/kg
Zinc 60g/kg；Iodine 200mg/kg；Selenium 100mg/kg
Surplus is medical stone.
The bacterium solution is S. cervisiae（Saccharomyces Cerevisiae ）CGMCC No.2388 bacterium solutions, withered grass
Bacillus（Bacillus subtilis）6928 bacterium solutions of CGMCC No. and lactobacillus acidophilus（Lactobacillus acidophilus）CGMCC No.5093 bacterium solution mixed bacteria liquids.
S. cervisiae in the bacterium solution（Saccharomyces Cerevisiae）Bacterium solution viable count >=3.0 × 109
A/mL, bacillus subtilis（Bacillus subtilis）Bacterium solution viable count >=5.0 × 109A/mL, lactobacillus acidophilus（Lactobacillus acidophilus）Viable count >=2.2 × 10 of bacterium solution10A/mL.
S. cervisiae（Saccharomyces Cerevisiae）2388 bacterium solutions of CGMCC No. are given birth to purchased from Qingdao root
Object Group Co., Ltd；
Bacillus subtilis（Bacillus subtilis）CGMCC No.6928 bacterium solutions, purchased from the azure biological group in Qingdao
Co., Ltd；
Lactobacillus acidophilus（Lactobacillus acidophilus）CGMCC No.5093 bacterium solutions are given birth to purchased from Qingdao root
Object Group Co., Ltd.
The activation method of the bacterium solution, summer：The weight ratio of above-mentioned three kinds of bacterium solutions presses 1:1:1 opportunistic pathogen mixed
1 part of liquid, 10 parts of molasses, 0.06 part of feed grade magnesium sulfate, 0.06 part of dipotassium hydrogen phosphate, 40 parts of room temperature tap water are kept for 35 DEG C -38
DEG C, stir-activating 5-6 hours；Winter：The weight ratio of above-mentioned three kinds of bacterium solutions presses 1:1:16 parts of the original bacteria liquid mixed, molasses 10
Part, 0.06 part of feed grade magnesium sulfate, 0.06 part of dipotassium hydrogen phosphate, 35 DEG C of 40 parts of -38 DEG C of tap water, heat preservation, stir-activating 6-8 are small
When.
Its production technology includes:（1）Raw material receives, is just clear, removes moulding material, blocky sundries；（2）Raw material crushes, powder
Particle degree is in 30 mesh or so；（3）Raw material mixes, inoculation, and prior activated bacterium solution is sprayed onto by sprinkling system on material,
It is mixed evenly；（4）It is packaged sealing, 8-12S peritonaeums or duplicature bag is taken to pack；（5）Constant heat storage ferments, 20 DEG C -40 DEG C
Fermentation 5-7 days.
A kind of feeding method of biological fermentation feed, the fermented feed additive amount：Grower pigs are being raised in the ratio of 20%-30%
This feed of Direct-fed before and after hello normal daily ration.
A kind of special equipment of biological fermentation feed, including raw material receive with just clear device, grinding device, mixing arrangement and
Packing apparatus；
It includes leading to receiving sieve for carrying out the circle of primary screening that the raw material, which is received with just clear device, and the circle leads to receiving sieve
Top be equipped with and be used for collecting the cloth bag pulse dust collector and centrifugal fan of raw material dust, the circle leads to the lower section of receiving sieve
It is cheated equipped with blanking；
The grinding device includes cheating the bucket elevator that be connected to blanking, and the pipe magnetic separator that is connected to bucket elevator top waits for
Feed bin is crushed, the lower section of feed bin to be comminuted is equipped with scroll feeder, and the lower section of scroll feeder is equipped with the hammer leaf powder communicated therewith
The lower section of broken machine, hammer crusher is equipped with screw conveyor；
The mixing arrangement includes fighter's elevator of screw conveyor connection, with the dispensing being connected at the top of fighter's elevator
The lower part in storehouse, batch bin is equipped with mixing bunker, and mixing bunker and small powder cast feeder, bacterium solution sprinkling system, high efficient mixer are respectively communicated with,
The bottom of mixing bunker is equipped with screw conveyor；
The packing apparatus includes the bucket elevator being connected to screw conveyor, the rotation being connected to bucket elevator point
The lower section of orchestration, rotary distributor is equipped with screw unloading storehouse, and the lower section in screw unloading storehouse is equipped with computer packaging scale.
By mechanization continuous production, solid fermentation in bag is effectively prevented biological fermentation feed of the present invention
Microbiological contamination in family workshop and solids bulk fermentation is gone mouldy problem, and product quality is stablized.The present invention is to utilize cheap agriculture
Crop leftover bits and pieces is turned waste into wealth by microbial solid fermentation, is formed a new generation and is collected nutrition, diseases prevention, material-saving, ecological quadruple effect in one
The full-nutrients biological fermented feed of body.The present invention is a kind of novel fodder, can be used as animal diets Direct-fed, and can substitute
20%~30% perfect compound feed is really realized and saves food, reduces aquaculture cost.Product of the present invention has special apple fragrant
Gas and lactic acid fragrance greatly improve the palatability of animal, increase feed intake.Contain a large amount of probiotics in product of the present invention, it can
To promote digestion of the animal to complete feed, efficiency of feed utilization is improved, maintains Tiny ecosystem colony balance in animal intestinal tract, is improved dynamic
Object immunity of organisms.
Description of the drawings
Attached drawing 1 is the structural schematic diagram of biological fermentation feed special equipment in embodiment.
In figure：101- circles lead to receiving sieve；102- cloth bag pulse dust collectors；103- centrifugal fans；104- blankings are cheated；
The first bucket elevators of 105-；201- pipe magnetic separators；202- feed bins to be comminuted；203- scroll feeders；204- hammer crushers；
205- screw conveyors；The second bucket elevators of 206-；301- batch bins；302- small powder cast feeders；303- mixing bunkers；304- high
Imitate mixing machine；305- bacterium solution sprinkling systems；306- screw conveyors；307- third bucket elevators；401- rotary distributors；
402-screw unloading storehouses；403-computer packaging scales.
Specific implementation mode
Embodiment 1
A kind of biological fermentation feed, including the score ratio mixed fermentation by weight of following components form：
100 parts of DDGS, 96 parts of apple pomace, 40 parts of date powder, 140 parts of peanut shell powder, 60 parts of maize peel of whitewashing, worm sand 100
Part, 20 parts of premix, 30 parts of bacterium solution, 6 parts of molasses, 408 parts of water.
Wherein：The premix includes following components by weight：600 parts of mountain flour, 20 parts of medical stone, compound vitamin 20
Part, 100 parts of compound micro- mine, 100 parts of calcium monohydrogen phosphate, 150 parts of salt, 5 parts of flavouring agent, 5 parts of sweetener；
The weight proportion of the wherein compound vitamin is as follows：
Vitamin A 5000KIU/kg vitamin Ds3 1000KIU/kg
Vitamin E 50000mg/kg vitamin Ks3 2500mg/kg
Vitamin B12000mg/kg vitamin Bs2 7500mg/kg
Vitamin B65000mg/kg vitamin Bs12 250mg/kg
Biotin 250mg/kg niacin 25000mg/kg
Calcium pantothenate 20000mg/kg folic acid 25mg/kg
Surplus is cornstarch；
The weight proportion of wherein compound micro- mine is as follows：
Copper 5g/kg；Iron 40g/kg；Manganese 30g/kg
Zinc 60g/kg；Iodine 200mg/kg；Selenium 100mg/kg
Surplus is medical stone.
Wherein bacterium solution is S. cervisiae（Saccharomyces Cerevisiae）CGMCC No.2388 bacterium solutions, withered grass
Bacillus（Bacillus subtilis）CGMCC No.4628 bacterium solutions and lactobacillus acidophilus（Lactobacillus acidophilus）CGMCC No.5093 bacterium solution mixed bacteria liquids.
Wherein S. cervisiae in bacterium solution（Saccharomyces Cerevisiae）Bacterium solution viable count >=3.0 × 109
A/mL, bacillus subtilis（Bacillus subtilis）Bacterium solution viable count >=5.0 × 109A/mL, lactobacillus acidophilus（Lactobacillus acidophilus）Viable count >=2.2 × 10 of bacterium solution10A/mL.
S. cervisiae（Saccharomyces Cerevisiae）CGMCC No.2388 bacterium solutions are given birth to purchased from Qingdao root
Object Group Co., Ltd；
Bacillus subtilis（Bacillus subtilis）CGMCC No.6928 bacterium solutions, purchased from the azure biological group in Qingdao
Co., Ltd；
Lactobacillus acidophilus（Lactobacillus acidophilus）CGMCC No.5093 bacterium solutions are given birth to purchased from Qingdao root
Object Group Co., Ltd.
The activation method of bacterium solution：
Summer：The weight ratio for three kinds of bacterium solutions that above-mentioned purchase comes presses 1:1:11 part of the original bacteria liquid mixed, 10 parts of molasses,
0.06 part of feed grade magnesium sulfate, 0.06 part of dipotassium hydrogen phosphate, 40 parts of room temperature tap water are kept for 35 DEG C -38 DEG C, stir-activating 5-6
Hour；Winter：Above-mentioned purchase carrys out the weight ratio of three kinds of bacterium solutions by 1:1:16 parts of the original bacteria liquid mixed, 10 parts of molasses, feed
0.06 part of magnesium sulfate of grade, 0.06 part of dipotassium hydrogen phosphate, 35 DEG C of 40 parts of -38 DEG C of tap water, heat preservation, stir-activating 6-8 hours.
A kind of preparation method of above-mentioned biological fermentation feed includes:
1, by raw material DDGS, apple pomace, date powder, peanut shell powder, maize peel of whitewashing, worm sand received respectively according to the ratio,
It is just clear, remove moulding material, blocky sundries；
2, just clear raw material is crushed respectively, grinding particle size is in 30 mesh or so；
3, raw material mixing just clear, after crushing, sterilizing, inoculation are carried out respectively.Prior activated bacterium solution is passed through into sprinkling
System is sprayed onto on material, is mixed evenly.Specific steps：Raw material after carrying out first cleer and peaceful crushing respectively fully enters mixed
It closes storehouse to finish, first opens bacterium solution sprinkling system steam threshold switch, lead to 30 minutes steam to pipeline, mixing bunker and the raw material crushed
Carry out disinfection sterilizing, prevents miscellaneous bacteria from being bred in feed and causes microbiological contamination so as to cause product moldy metamorphism after bag is packed into.This process
It is to maintain the critical control point of product quality stabilization.After having led to steam, added on one side by bacterium solution sprinkling system by formula rate
Fresh tap water, summer are directly added into room temperature tap water, and 30 DEG C of warm water is added in winter, and warm water, which is added, so that viable bacteria is comparatively fast fitted
New growing environment, tachyauxesis breeding is answered to shorten fermentation period, improve product quality.It is put on one side by small powder cast feeder pre-
Batch mixing（Premix cannot prevent trace element rotten by steam sterilizing）.After the completion of feeding intake and adding water, bacterium solution sprinkling system is waited for
Channel temp under the overall leadership be cooled to 40 DEG C hereinafter, start again spray bacterium solution, prevent scald strain.It stirs, sprays 10 seconds in sprinkling,
Stirring 30 seconds, then spray 10 seconds, it is stirred for 30 seconds, until bacterium solution is sprayed, remixes stirring 90 seconds, ensure mixing
Uniformly.The more existing fermented feed technique of this technological process is the difference is that increase one of sterilizing program in kind, by right
The improvement of bacterium solution sprinkling system increases the pipeline and control system of a set of logical steam, can be to bacterium solution pipeline, mixing bunker, original
Material carries out sterilization, prevents microbiological contamination.In addition this technique ensure that the nutritional ingredients such as trace element, minerals are not destroyed, bacterium
Kind survival rate does not decline, and reproduction speed is fast, fermentation period is short, and material stirring mixture homogeneity is high, and product quality is stablized.
4, it is packaged sealing, 8-12S peritonaeums or duplicature bag is taken to pack.Due to moisture content of material height, spiral is used
Formula discharge bin ensure that the good fluidity of material；Food bag is packed using 8-12S peritonaeums or duplicature bag, and inner bag is using by hand
Tying, outer bag use sack closer, 2 kilograms of pressure in bag.The advantages of this packaging bag be can reach packaging bag be neither easy it is outer
The rupture of portion pressure extrusion, and be not easy to make packaging bag because of gases such as viable bacteria growth metabolism generates in bag exhaust gas such as carbon dioxide
It bursts, sealing effect can reach the air of outside into not going, the optimal seal effect that the exhaust gas of the inside can be discharged in right amount.
5, constant heat storage ferments, and 20 DEG C -40 DEG C ferment 5-7 days, finished product.
Above-mentioned biological fermentation feed is prepared by following special equipment in the present embodiment, including raw material receive with just
Clear device, grinding device, mixing arrangement and packing apparatus；
It includes leading to receiving sieve 101 for carrying out the circle of primary screening that the raw material, which is received with just clear device, and the circle is logical just
The top of cleaning 101 is equipped with the cloth bag pulse dust collector 102 and centrifugal fan 103 for being used for collecting raw material dust, the circle
The lower section of logical receiving sieve 101 is equipped with blanking and cheats 104；
The grinding device includes the first bucket elevator 105 being connected to blanking hole 104, with the first bucket elevator
The lower section of pipe magnetic separator 201, the feed bin to be comminuted 202 that 105 tops are connected to, feed bin 202 to be comminuted is equipped with scroll feeder 203, spiral shell
The lower section of rotation feeder 203 is equipped with the hammer crusher 204 communicated therewith, and the lower section of hammer crusher 204 is equipped with helical feed
Machine 205；
The mixing arrangement includes the second bucket elevator 206 that screw conveyor 205 is connected to, with the second bucket elevator
The lower part of the batch bin 301 that 206 tops are connected to, batch bin 301 is equipped with mixing bunker 303, and 303 the inside of mixing bunker is equipped with efficiently mixing
Machine 304, mixing bunker 303 are respectively communicated with small powder cast feeder 302, bacterium solution sprinkling system 305, and the bottom of mixing bunker 303 is equipped with spiral shell
Revolve conveyer 306；
The packing apparatus includes the third bucket elevator 307 being connected to screw conveyor 306, with third bucket lifting
The lower section of the rotary distributor 401 that machine 307 is connected to, rotary distributor 401 is equipped with screw unloading storehouse 402, screw unloading storehouse 402
Lower section is equipped with computer packaging scale 403.
The technique that biological fermentation feed is produced using above equipment is as follows：
Raw material enters the logical receiving sieve 101 of circle through feeding port, enters blanking hole 104 through the blocky sundries of primary screening removal, throws
Raw material dust is collected by cloth bag pulse dust collector 102 during material.
Raw material in blanking hole 104 is by the first bucket elevator 105, and into feed bin 202 to be comminuted, raw material is into powder
Iron impurities are removed through pipe magnetic separator 201 before particle storehouse, material is sent by feed bin 202 to be comminuted by scroll feeder 203 and is hammered into shape
Tablet pulverizer 204, material crushes in hammer crusher, and control grinding particle size is in 30 mesh or so.
Raw material after crushing enters fighter's elevator 206 by screw conveyor 205 and is promoted to batch bin 301, waits owning
Bulk raw material finishes after above-mentioned technique into batch bin respectively not comprising premix, bacterium solution, molasses and water.
Opening batch bin discharge valve makes raw material enter mixing bunker 303, waits for that material enters mixing bunker and finishes, and opens bacterium solution sprinkling
305 steam threshold switch of system, logical steam carry out disinfection sterilizing to pipeline, mixing bunker and the raw material that crushes, and steam to be led to finishes,
Tap water enters mixing bunker by bacterium solution sprinkling system, while putting into premix by small powder cast feeder 302, waits the water that feeds intake and add
After the completion, after bacterium solution sprinkling system pipe temperature is cooled to 40 DEG C or less.
Prior activated bacterium solution is sprayed at by bacterium solution sprinkling system 305 on material, and the spray of 304 side of high efficient mixer is started
Side stirring is spilt, the mixed processes of material are completed.Mixed material will by screw conveyor 306, third bucket elevator 307
Material is sent to rotary distributor 401, and material is assigned to screw unloading storehouse 402 by rotary distributor 401.
The material to be got off by screw unloading storehouse 402 enters computer packaging scale 403 and weighs pack, and inner bag carries out artificial tying
Afterwards, outer bag is sealed by sack closer.Material after package encapsulation is sent into ferment at constant temperature library, carries out fermentation 5-7 days, is made into
Product.
The microbial fermented fodder nutrient ingredient of above-mentioned preparation is measured：
Assay method：
The CP in feed assay method GB/T6432
Coarse-fibred assay GB/T6434 in feed
The measurement GB/T6435 of moisture and other volatile matter contents in feed
Calcium catalyst GB/T6436 in feed
Total phosphorus yield spectrophotometry GB/T6437 in feed
The assay method GB/T6438 of feed coarse ash
Fodder yeast viable count GB/T 22547
The measurement GB/T6439 of water soluble chloride in feed
Prolease activity measures：
Claim drying 1.000 g of sample in triangular flask, adds 7.2 phosphate buffers of pH, 20 mL, 40 DEG C of water-bath heat preservations 60
Min selects suitable extension rate to measure the activity of protease using folin's methods after filtering.By 1g enzyme powders every 1 at 40 DEG C
The enzyme activity that min hydrolysed caseins generate 1 μ g tyrosine is defined as 1U.
Cellulase activity measures：
Claim drying 0.500 g of sample in triangular flask, adds 20 mL of distilled water, 40 DEG C of water-baths keep the temperature 45 min, after filtering
Select suitable extension rate carboxymethylcellulose sodium（CMC）Substrate is done, DNS is used（3,5- dinitrosalicylic acids）Method measures also
Raw sugar content calculates cellulase activity.CMC will be hydrolyzed in 1 min per 1g cellulase in the case where 5.0,40 DEG C of pH,
The enzymatic activity for generating 1 μ g glucose is defined as 1U.
Phytase activity measures：
Claim drying 0.500 g of sample in triangular flask, adds 5.5 acetic acid-sodium acetate buffer solutions of pH, 20 mL, 40 DEG C of water-baths
30 min select suitable extension rate ammonium metavanadate method to measure the vigor of phytase after filtering.Phytic acid enzyme sample is in phytic acid
Na concn is 0.005 mol/L, and under conditions of 40 DEG C of temperature, pH 5.50, it is inorganic that 1 μm of ol is discharged from sodium phytate per 1min
Phosphorus, as a phytase activity are defined as 1U.
1#, 2#, 3# sample obtained is taken to be measured, measurement result is as follows：
The measurement result of various compositions in 1 biological fermentation feed of table
As seen from Table 1, crude protein content 30%-35% in fermented feed, and crude fibre≤10%, coarse ash≤10%, calcium
Content 0.5%-1.2%, total phosphorus >=0.2%, sodium chloride content 0.2%-0.9%, moisture 40%-45%, number of viable >=5,000,000,000/
Gram.
The variation of hydrolase after the fermentation of 2 biological fermentation feed of table
As can be seen from Table 2, in fermented feed proteinase activity be 550-650U, cellulase activity 5900-6000U,
Phytase activity is 120-150U.
The various hydrolytic enzyme activities of the fermented feed after bacterium are connect to greatly improve, the cellulose that is rich in the feedstuff that can degrade,
Hemicellulose is difficult to the organophosphors such as the albumen digested and assimilated, phytate phosphorus, convert it into conducive to digest and assimilate small molecular sugar,
Small peptide, amino acid and Phos.
Feeding method of the above-mentioned biological fermentation feed to grower pigs：
Grower pigs feeding trial：
Test period：1 day to 2012 July in 2012, on September 30, amounted to 92 days.
The source of test pig and grouping：It all is from Shandong Province Pingdu City south villages and small towns pig farm；Control group is using in the market
The complete feed of purchase, test group are that fermented feed is directly raised as the perfect compound feed of growth swine rations replacement 20%-30%
Hello.
Test site：Shandong Province Pingdu City south villages and small towns pig farm.
Test result：
Using automatic diet machine for food.Test group is total to use 1900kg feeds, wherein using biological fermentation feed
475kg.Control group is total to use 1600kg complete feeds.
3 biological fermentation feed grower pigs of table feed application test
As seen from Table 3, the perfect compound feed Direct-fed for 25% being substituted with biological fermentation feed educates grower pigs, improves
The speed of the feed intakes of grower pigs, pig fattening accelerates；Due in fermented feed albumen and energy be not enough to reach full price
Feed contents are equivalent to pig after eating up staple food and have eaten and order snacks, therefore feedstuff-meat ratio relative increase, but this biological fermentation feed
Cheap, 1400 yuan of producer price per ton is used in mixed way raising assembly relative to average 3200 yuan/ton of perfect compound feed
This reduces 10.3% instead.
Claims (1)
1. a kind of biological fermentation feed, it is characterised in that：The biological fermentation feed, including following components are by ratio of weight and the number of copies
Mixed fermentation forms：100 parts of DDGS, 96 parts of apple pomace, 40 parts of date powder, 140 parts of peanut shell powder, 60 parts of maize peel of whitewashing, worm are husky
100 parts, 20 parts of premix, 30 parts of bacterium solution, 6 parts of molasses, 408 parts of water；
Crude protein content 30%-35% in the fermented feed, crude fibre≤10%, coarse ash≤10%, calcium content 0.5%-1.2%,
Total phosphorus >=0.2%, sodium chloride content 0.2%-0.9%, moisture 40%-45%, number of viable >=5,000,000,000/gram；
Proteinase activity is 550-650U, cellulase activity 5900-6000U in the fermented feed, and phytase activity is
120-150U；
The premix includes following components by weight：600 parts of mountain flour, 20 parts of medical stone, 20 parts of compound vitamin, compound micro- mine
100 parts, 100 parts of calcium monohydrogen phosphate, 150 parts of salt, 5 parts of flavouring agent, 5 parts of sweetener；
The weight proportion of the wherein compound vitamin is as follows：
Vitamin A 5000KIU/kg vitamin Ds3 1000KIU/kg
Vitamin E 50000mg/kg vitamin Ks3 2500mg/kg
Vitamin B12000mg/kg vitamin Bs2 7500mg/kg
Vitamin B65000mg/kg vitamin Bs12 250mg/kg
Biotin 250mg/kg niacin 25000mg/kg
Calcium pantothenate 20000mg/kg folic acid 25mg/kg
Surplus is cornstarch；
The weight proportion of wherein compound micro- mine is as follows：
Copper 5g/kg；Iron 40g/kg；Manganese 30g/kg；
Zinc 60g/kg；Iodine 200mg/kg；Selenium 100mg/kg；
Surplus is medical stone；
The bacterium solution is S. cervisiaeSaccharomyces CerevisiaeCGMCC No.2388 bacterium solutions, bacillus subtilis
BacteriumBacillus subtilisCGMCC No.6928 bacterium solutions and lactobacillus acidophilusLactobacillus acidophilus
CGMCC No.5093 bacterium solution mixed bacteria liquids；
The activation method of bacterium solution：
Summer：The weight ratio for three kinds of bacterium solutions that above-mentioned purchase comes presses 1:1:11 part of the original bacteria liquid mixed, 10 parts of molasses, feed
0.06 part of magnesium sulfate of grade, 0.06 part of dipotassium hydrogen phosphate, 40 parts of room temperature tap water are kept for 35 DEG C -38 DEG C, stir-activating 5-6 hours；
Winter：Above-mentioned purchase carrys out the weight ratio of three kinds of bacterium solutions by 1:1:16 parts of the original bacteria liquid mixed, 10 parts of molasses, feed grade sulfuric acid
0.06 part of magnesium, 0.06 part of dipotassium hydrogen phosphate, 35 DEG C of 40 parts of -38 DEG C of tap water, heat preservation, stir-activating 6-8 hours；
A kind of preparation method of above-mentioned biological fermentation feed includes:
1）, by raw material DDGS, apple pomace, date powder, peanut shell powder, maize peel of whitewashing, worm sand is received, just respectively according to the ratio
Clearly, moulding material, blocky sundries are removed；
2）, just clear raw material crushed respectively, grinding particle size is in 30 mesh or so；
3）, respectively carry out it is just clear, crush after raw material mixing, sterilizing, inoculation；Raw material after carrying out first cleer and peaceful crushing respectively is complete
Portion enters mixing bunker and finishes, and first opens bacterium solution sprinkling system steam threshold switch, leads to 30 minutes steam to pipeline, mixing bunker and crushing
Good raw material carries out disinfection sterilizing；After having led to steam, formula rate is pressed on one side, fresh tap water is added by bacterium solution sprinkling system,
Summer is directly added into room temperature tap water, and 30 DEG C of warm water is added in winter；
Premix is put by small powder cast feeder on one side, after the completion of feeding intake and adding water, waits for that bacterium solution sprinkling system pipe temperature cools down
To 40 DEG C hereinafter, starting to spray bacterium solution again, scald strain is prevented；It sprays 10 seconds, is stirred for 30 seconds again, until bacterium solution is sprayed
It finishes, remixes stirring 90 seconds,
4）, be packaged sealing, 2 kilograms of pressure in bag；
5）, constant heat storage fermentation, 20 DEG C -40 DEG C ferment 5-7 days, finished product；
The biological fermentation feed is prepared by following special equipment, including raw material is received and filled with just clear device, crushing
It sets, mixing arrangement and packing apparatus.
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| CN104856204A (en) | 2015-08-26 |
| CN104938767B (en) | 2018-05-22 |
| CN104839428A (en) | 2015-08-19 |
| CN103250868A (en) | 2013-08-21 |
| CN104938789A (en) | 2015-09-30 |
| CN103250868B (en) | 2015-09-23 |
| CN104856204B (en) | 2017-06-16 |
| CN104938767A (en) | 2015-09-30 |
| CN104839428B (en) | 2018-11-20 |
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