CN104931445B - It is a kind of for postpartum ischemic, lochia Chinese medicine composition detection method of content - Google Patents
It is a kind of for postpartum ischemic, lochia Chinese medicine composition detection method of content Download PDFInfo
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Abstract
It is a kind of for postpartum ischemic, the detection method of content of the Chinese medicine composition of lochia it is an object of the invention to disclose, it is characterised in that contain following content assaying method in this method：Ferrous sulfate reference substance 0.4g is taken, it is accurately weighed, add sulfuric acid solution to dissolve, precision measures 2ml, obtains reference substance solution；Precision measures reference substance solution, sets respectively in 25ml measuring bottles, adds water to 10ml, then adds 1% hydroxylamine hydrochloride solution 1ml and 0.2%2,2 bipyridyl 1ml, and mixing draws standard curve；Precision measures this product, and it is 3.0~5.5 to instill 0.5~5% sodium hydroxide solution to PH, adds 1% hydroxylamine hydrochloride solution 1ml and 0.2%2,2 bipyridyl ethanol solution 1ml, measures absorbance, from the weight for reading ferrous sulfate in test solution on standard curve.Technical problem to be solved by the present invention lies in the defects for improving original content assaying method, improve the accuracy and controllability of product quality.
Description
One, technical field：The present invention relates to a kind of detection method of content, more particularly to one kind is for postpartum ischemic, lochia
Chinese medicine composition detection method of content.
Two, background technology：
Anaemia caused by a variety of causes iron deficiency that hypoferric anemia refers to causes RBC acceptor garland rate to reduce refers to body to iron
Demand and supply it is unbalance, lead to store that iron loss is most in vivo, followed by Erythrocyte Iron lack so as to cause anaemia, iron-deficient is poor
Blood is most common anaemia.This disease is common type in anaemia, is prevalent in all over the world.According to the statistics of the World Health Organization：
There are about the different degrees of anaemias of 3,000,000,000 people in the whole world, and number dead due to suffering from anaemia and being led to various diseases is up to ten million every year.Middle national trouble
The population probability of anaemia is higher than western countries, and in the crowd for suffering from anaemia, women is apparently higher than male, during old man and children are higher than
It is young.
More due to losing blood relatively in stages of labor, postpartum relatively easily causes puerpera's anaemia.Anaemia not only influences puerpera's physical recovery
Speed, and affect to newborn nutrition offer, therefore early prevention and treatment plays an important roll to preventing postpartum anemia.
Based on malaise, loss of appetite, resistance decline severe complication can occur for postpartum anemia common symptoms, face
Bed is slight, and postpartum anemia (90g/L) can generally be corrected by diet and can be improved.But moderate postpartum anemia (60-90g/L) removes
Dietary adjustments external demand adds drug and is treated.Common oral benefit iron drug has certain gastrointestinal side effect more, such as dislikes
The patients such as the heart, vomiting, anorexia are forced to be discontinued in the case of must not being resistant to.Even more cause puerpera's physiology, psychological torment
And pain.
Iron supplementary in the market is based on Western medicine, it only carries out merely the supplement of iron, while its toxic side effect usually enables
Patient puzzles；Some Chinese medicine iron supplementaries are usually because being the inaccurate curative effect of health products and patient being allowed to be at a loss.It enriches blood general
The store-through problem low in iron absorptivity takes effect too slow；Using simple chemical iron as main component, dosage is difficult to slap some products
It holds, excessive irony is deposited in brain tissue, bone, damage health；Many enrich blood product can stimulating gastrointestinal, mended blood and hindered
Stomach；Mouthfeel is bad, and iron taste weight, people are reluctant to eat.Treatment anaemia is strengthening the spleen and replenishing qi at all, restores the transporting work(of taste
Can, promote being absorbed and utilized for iron.Tangible blood is born in invisible gas.Blood nourishing oral liquid is exactly enriching the blood and tonifying qi, strengthening the spleen and stomach, with
The source of abundant anger；It is equipped with jujube to enrich blood, then Yin grows while yang is generating, the symptom of anaemia is improved rapidly.Sea-buckthorn have it is apparent anti-oxidant and
Reduce hyperlipidemia.Treatment for anaemia, mostly uses ferrotherapy both at home and abroad at present.But take to long-term, high-dose chalybeate institute
The side effect brought, such as anorexia, Nausea and vomiting, diarrhea etc. is not easy to be received by patient.
The formulation ingredients of Chinese medicine are relatively more, and pre-treatment difficulty is bigger, and interference is more.Detection method is advanced, eliminates
Interference, precision and favorable reproducibility comprehensively can carry out quality control to the drug, ensure product quality stability,
Homogeneity.About iron-containing composition, in the content assaying method of the prior art, the content for going out active principle of measurement is relatively low,
It is inaccurate, it is difficult to which that science accurately controls the quality of product.
Three, invention content：
It is a kind of for postpartum ischemic, the detection method of content of the Chinese medicine composition of lochia present invention aims at providing
Purpose of the present invention is to be achieved through the following technical solutions：
The content assaying method of drug Chinese medicinal composition preparation of the present invention includes：
Ferrous sulfate reference substance 0.4g is taken, it is accurately weighed, it sets in 100ml measuring bottles, adds sulfuric acid solution (1 → 20) 1ml and water
80ml makes it dissolve, and adds water to scale, shakes up, and precision measures 2ml, sets in 100ml measuring bottles, is diluted with water to scale, shake up, i.e.,
Obtain reference substance solution；Precision measures reference substance solution 1.0ml, 2.0ml, 4.0ml, 6.0ml, 8.0ml, sets 25ml measuring bottles respectively
In, 10ml is added water to, then add 1% hydroxylamine hydrochloride solution 1ml and 0.2%2,2- bipyridyl 1ml, mixing adds water to scale, shakes
It is even, using corresponding solution as blank, according to UV-VIS spectrophotometry, absorbance is measured at 522nm wavelength, with absorbance
For ordinate, a concentration of abscissa draws standard curve；Precision measures 5~15ml of this product, sets in 100ml measuring bottles, instills
0.5%~5% sodium hydroxide solution is 3.0~5.5 to PH, adds water to scale, shakes up, and precision measures 5ml, sets 25ml measuring bottles
In, 10ml is added water to, then add 1% hydroxylamine hydrochloride solution 1ml and 0.2%2,2- bipyridyl ethanol solution 1ml, mixing adds water to
Scale shakes up, and using corresponding solution as blank, measures absorbance, the ferrous sulfate from reading test solution on standard curve
Weight, calculate to get；
The content assaying method of drug Chinese medicinal composition preparation of the present invention preferably includes：
Ferrous sulfate reference substance 0.4g is taken, it is accurately weighed, it sets in 100ml measuring bottles, adds sulfuric acid solution (1 → 20) 1ml and water
80ml makes it dissolve, and adds water to scale, shakes up, and precision measures 2ml, sets in 100ml measuring bottles, is diluted with water to scale, shake up, i.e.,
Obtain reference substance solution；Precision measures reference substance solution 1.0ml, 2.0ml, 4.0ml, 6.0ml, 8.0ml, sets 25ml measuring bottles respectively
In, 10ml is added water to, then add 1%~2% hydroxylamine hydrochloride solution 1ml and 0.2%2,2- bipyridyl 1ml, mixing adds water to quarter
Degree, shakes up, and using corresponding solution as blank, according to UV-VIS spectrophotometry, absorbance is measured at 522nm wavelength, to inhale
Luminosity is ordinate, a concentration of abscissa, draws standard curve；Precision measures this product 10ml, sets in 100ml measuring bottles, instills 1%
Sodium hydroxide solution is 3.5~4.5 to PH, adds water to scale, shakes up, and precision measures 5ml, sets in 25ml measuring bottles, adds water to
10ml, then add 1% hydroxylamine hydrochloride solution 1ml and 0.2%2,2- bipyridyl ethanol solution 1ml, mixing adds water to scale, shakes up,
Using corresponding solution as blank, absorbance is measured, from the weight for reading ferrous sulfate in test solution on standard curve, meter
Calculate to get.
This product contains melanterite per 10ml in terms of ferrous sulfate, must not be less than 45mg.
Of the invention signified is for postpartum ischemic, the bulk pharmaceutical chemicals of the Chinese medicine composition of lochia and proportioning：(by weight)
2~8 parts by weight of melanterite, 180~220 parts by weight of sea-buckthorn, 80~120 parts by weight of hawthorn, 80~120 weight of jujube
Part.
The bulk pharmaceutical chemicals composition optimum ratio of drug combination preparation of the present invention is (by weight):
5.3 parts by weight of melanterite, 200 parts by weight of sea-buckthorn, 100 parts by weight of hawthorn, 100 parts by weight of jujube.
Clinically-acceptable dosage form can be conventionally made in the above four traditional Chinese medicine, as tablet, granule, capsule,
Oral liquid, dripping pill, controlled release preparation, injection.
The preparation method of this drug combination preparation can be:
Sea-buckthorn, hawthorn, three taste medicine of jujube is taken to add water to cook 2 times, for the first time plus 10 times of amounts of water decoct 1.5 hours, the second system
8 times of amounts of water are added to decoct 1 hour, collecting decoction filters, and filtrate is concentrated into every 1ml crude drugs containing 1g, lets cool, ethyl alcohol is added to make alcohol content
It is 70%, stirs evenly, stand overnight, leaching supernatant, filtrate recycling ethanol is spare to no alcohol taste；Melanterite adds 0.1% sulphur
Acid solution 50ml, stirs to dissolve, and vitamin C 5g is added in filtration, filtrate, is stirred to dissolve, solution for standby；Take sucrose
200g adds suitable quantity of water, and simple syrup is made, and above-mentioned two kinds of liquids are added in filtration, and mixing takes 0.2~0.8g of sodium hydroxide, are added
It in above-mentioned admixing medical solutions, stirs evenly, pH value is adjusted to 2.5-4.0 with citric acid, is added to 1000ml, stirs evenly, stand, filter, fill
Dress, sterilizing to get.
The preparation method of this drug combination preparation is preferably:
Sea-buckthorn, hawthorn, three taste medicine of jujube is taken to add water to cook 2 times, for the first time plus 10 times of amounts of water decoct 1.5 hours, second
8 times of amounts of water are added to decoct 1 hour, collecting decoction filters, and filtrate is concentrated into every 1ml crude drugs containing 1g, lets cool, ethyl alcohol is added to make alcohol content
It is 70%, stirs evenly, stand overnight, leaching supernatant, filtrate recycling ethanol is spare to no alcohol taste；Melanterite adds 0.1% sulphur
Acid solution 50ml, stirs to dissolve, and vitamin C 5g is added in filtration, filtrate, is stirred to dissolve, solution for standby；Take sucrose
200g adds suitable quantity of water, and simple syrup is made, filtration, and above-mentioned two kinds of liquids are added, and mixing takes sodium hydroxide 0.56g, is added above-mentioned
It in admixing medical solutions, stirs evenly, pH value is adjusted to 2.5-4.0 with citric acid, is added to 1000ml, stirs evenly, stand, filtration is filling, goes out
Bacterium to get.
The preparation method of this drug combination preparation can also be:
Sea-buckthorn, hawthorn, three taste medicine of jujube is taken to add water to cook 2 times, for the first time plus 10 times of amounts of water decoct 1.5 hours, second
8 times of amounts of water are added to decoct 1 hour, collecting decoction filters, and filtrate is concentrated into every 1ml crude drugs containing 1g, lets cool, ethyl alcohol is added to make alcohol content
It is 70%, stirs evenly, stand overnight, leaching supernatant, filtrate recycling ethanol is spare to no alcohol taste；Melanterite adds 0.1% sulphur
Acid solution 50ml, stirs to dissolve, and vitamin C 5g is added in filtration, filtrate, is stirred to dissolve, solution for standby；Take sucrose
200g adds suitable quantity of water, and simple syrup is made, and above-mentioned two kinds of liquids are added in filtration, and mixing takes sodium benzoate 2g, it is added above-mentioned mixed
It closes in liquid, stirs evenly, pH value is adjusted to 2.5-4.0 with citric acid, is added to 1000ml, stirs evenly, stand, filtration is filling, goes out
Bacterium to get.
Technical problem to be solved by the present invention lies in the defects for improving original content assaying method, improve product quality
Accuracy and controllability.The Chinese medicine composition that the present invention is studied is made of melanterite, sea-buckthorn, hawthorn, jujube, melanterite conduct
The monarch drug in a prescription of we, main component iron is also the active ingredient of this product, in order to control the quality of drug combination preparation of the present invention,
Ensureing its curative effect, the content assaying method that the present invention uses carries out the assay of iron, and the content method of iron is more scientific and reasonable,
Data are more acurrate.Content assaying method through the invention improves the accuracy of product qualified products, reduces waste, reduces
Cost is conducive to the quality control of industrialized production.
Experimental example and embodiment are for further illustrating but being not limited to the present invention below
Experimental example 1：The contrast experiment of detection method of content and the content method of the present invention in the prior art：It is embodiment to take
1,2,3,4 sample carries out assay respectively.
Content assaying method one：Assay is carried out according to content assaying method in the prior art：
Ferrous sulfate reference substance 0.4g is taken, it is accurately weighed, it sets in 100ml measuring bottles, adds sulfuric acid solution (1 → 20) 1ml and water
80ml makes it dissolve, and adds water to scale, shakes up, and precision measures 2ml, sets in 100ml measuring bottles, is diluted with water to scale, shake up, i.e.,
Obtain reference substance solution (sulfur acid ferrous iron 80ug in per 1ml)；Precision measure reference substance solution 1.0ml, 2.0ml, 4.0ml,
6.0ml, 8.0ml are set respectively in 25ml measuring bottles, add water to 10ml, then 1% hydroxylamine hydrochloride solution 1ml and 0.2%2,2- is added to join pyrrole
Pyridine 1ml, mixing add water to scale, shake up, using corresponding solution as blank, according to UV-VIS spectrophotometry (Chinese Pharmacopoeia
The one annex V A of version in 2010), absorbance is measured at 522nm wavelength, using absorbance as ordinate, a concentration of abscissa is painted
Standard curve processed；Precision measures this product 10ml, sets in 100ml measuring bottles, adds water to scale, shakes up, and precision measures 10ml, sets 50ml
In measuring bottle, scale is added water to, is shaken up, precision measures 5ml, sets in 25ml measuring bottles, adds water to 10ml, then add 1% hydroxylamine hydrochloride molten
Liquid 1ml and 0.2%2,2- bipyridyl ethanol solution 1ml, mixing adds water to quarter, shakes up, and using corresponding solution as blank, measures
Absorbance, from standard curve read test solution in ferrous sulfate weight, calculate to get.
Content assaying method two：Content assaying method according to the invention carries out assay：
Ferrous sulfate reference substance 0.4g is taken, it is accurately weighed, it sets in 100ml measuring bottles, adds sulfuric acid solution (1 → 20) 1ml and water
80ml makes it dissolve, and adds water to scale, shakes up, and precision measures 2ml, sets in 100ml measuring bottles, is diluted with water to scale, shake up, i.e.,
Obtain reference substance solution；Precision measures reference substance solution 1.0ml, 2.0ml, 4.0ml, 6.0ml, 8.0ml, sets 25ml measuring bottles respectively
In, 10ml is added water to, then add 1% hydroxylamine hydrochloride solution 1ml and 0.2%2,2- bipyridyl 1ml, mixing adds water to scale, shakes
It is even, using corresponding solution as blank, according to UV-VIS spectrophotometry, absorbance is measured at 522nm wavelength, with absorbance
For ordinate, a concentration of abscissa draws standard curve；Precision measures this product 10ml, sets in 100ml measuring bottles, and instillation 1%~
2% sodium hydroxide solution is 3.5~4.5 to PH, adds water to scale, shakes up, and precision measures 5ml, sets in 25ml measuring bottles, adds water
To 10ml, then add 1% hydroxylamine hydrochloride solution 1ml and 0.2%2,2- bipyridyl ethanol solution 1ml, mixing adds water to scale, shakes
It is even, using corresponding solution as blank, absorbance is measured, the weight of ferrous sulfate from reading test solution on standard curve,
Calculate to get.
As a result as follows：
According to result it is found that the measurement result for measuring ratio method one with the sample iron content that method two carries out is higher, statistics
It is examined using t, compared with method one, method two has difference, P ＜ 0.05.
(3) brief summary：The assay carried out using the present invention is qualified products, but due to the assay of the prior art
The defect of method causes qualified products detection mistake, causes the raising of the waste of product and cost, the also control without forensic science
The quality of product processed.
Embodiment 1：
Melanterite 5.3g, sea-buckthorn 200g, hawthorn 100g, jujube 100g
Sea-buckthorn, hawthorn, three taste medicine of jujube is taken to add water to cook 2 times, for the first time plus 10 times of amounts of water decoct 1.5 hours, second
8 times of amounts of water are added to decoct 1 hour, collecting decoction filters, and filtrate is concentrated into every 1ml crude drugs containing 1g, lets cool, ethyl alcohol is added to make alcohol content
It is 70%, stirs evenly, stand overnight, leaching supernatant, filtrate recycling ethanol is spare to no alcohol taste；Melanterite adds 0.1% sulphur
Acid solution 50ml, stirs to dissolve, and vitamin C 5g is added in filtration, filtrate, is stirred to dissolve, solution for standby；Take sucrose
200g adds suitable quantity of water, and simple syrup is made, and above-mentioned two kinds of liquids are added in filtration, and mixing takes sodium benzoate 2g, it is added above-mentioned mixed
It closes in liquid, stirs evenly, pH value is adjusted to 2.5-4.0 with citric acid, is added to 1000ml, stirs evenly, stand, filtration is filling, goes out
Bacterium to get.
Ferrous sulfate reference substance 0.4g is taken, it is accurately weighed, it sets in 100ml measuring bottles, adds sulfuric acid solution (1 → 20) 1ml and water
80ml makes it dissolve, and adds water to scale, shakes up, and precision measures 2ml, sets in 100ml measuring bottles, is diluted with water to scale, shake up, i.e.,
Obtain reference substance solution；Precision measures reference substance solution 1.0ml, 2.0ml, 4.0ml, 6.0ml, 8.0ml, sets 25ml measuring bottles respectively
In, 10ml is added water to, then add 1% hydroxylamine hydrochloride solution 1ml and 0.2%2,2- bipyridyl 1ml, mixing adds water to scale, shakes
It is even, using corresponding solution as blank, according to UV-VIS spectrophotometry, absorbance is measured at 522nm wavelength, with absorbance
For ordinate, a concentration of abscissa draws standard curve；Precision measures this product 10ml, sets in 100ml measuring bottles, and instillation 1%~
2% sodium hydroxide solution is 3.5~4.5 to PH, adds water to scale, shakes up, and precision measures 5ml, sets in 25ml measuring bottles, adds water to
10ml, then add 1% hydroxylamine hydrochloride solution 1ml and 0.2%2,2- bipyridyl ethanol solution 1ml, mixing adds water to scale, shakes up,
Using corresponding solution as blank, absorbance is measured, from the weight for reading ferrous sulfate in test solution on standard curve, meter
Calculate to get.
This product contains melanterite per 10ml in terms of ferrous sulfate, is 49.23mg.
Embodiment 2：
Melanterite 5.3g, sea-buckthorn 200g, hawthorn 100g, jujube 100g
Sea-buckthorn, hawthorn, three taste medicine of jujube is taken to add water to cook 2 times, for the first time plus 10 times of amounts of water decoct 1.5 hours, the second system
8 times of amounts of water are added to decoct 1 hour, collecting decoction filters, and filtrate is concentrated into every 1ml crude drugs containing 1g, lets cool, ethyl alcohol is added to make alcohol content
It is 70%, stirs evenly, stand overnight, leaching supernatant, filtrate recycling ethanol is spare to no alcohol taste；Melanterite adds 0.1% sulphur
Acid solution 50ml, stirs to dissolve, and vitamin C 5g is added in filtration, filtrate, is stirred to dissolve, solution for standby；Take sucrose
200g adds suitable quantity of water, and simple syrup is made, filtration, and above-mentioned two kinds of liquids are added, and mixing takes sodium hydroxide 0.56g, is added above-mentioned
It in admixing medical solutions, stirs evenly, pH value is adjusted to 2.5-4.0 with citric acid, is added to 1000ml, stirs evenly, stand, filtration is filling, goes out
Bacterium to get.
Ferrous sulfate reference substance 0.4g is taken, it is accurately weighed, it sets in 100ml measuring bottles, adds sulfuric acid solution (1 → 20) 1ml and water
80ml makes it dissolve, and adds water to scale, shakes up, and precision measures 2ml, sets in 100ml measuring bottles, is diluted with water to scale, shake up, i.e.,
Obtain reference substance solution；Precision measures reference substance solution 1.0ml, 2.0ml, 4.0ml, 6.0ml, 8.0ml, sets 25ml measuring bottles respectively
In, 10ml is added water to, then add 1% hydroxylamine hydrochloride solution 1ml and 0.2%2,2- bipyridyl 1ml, mixing adds water to scale, shakes
It is even, using corresponding solution as blank, according to UV-VIS spectrophotometry, absorbance is measured at 522nm wavelength, with absorbance
For ordinate, a concentration of abscissa draws standard curve；Precision measures this product 10ml, sets in 100ml measuring bottles, and instillation 1%~
2% sodium hydroxide solution is 3.5~4.5 to PH, adds water to scale, shakes up, and precision measures 5ml, sets in 25ml measuring bottles, adds water to
10ml, then add 1% hydroxylamine hydrochloride solution 1ml and 0.2%2,2- bipyridyl ethanol solution 1ml, mixing adds water to scale, shakes
It is even, using corresponding solution as blank, absorbance is measured, the weight of ferrous sulfate from reading test solution on standard curve,
Calculate to get.
This product contains melanterite per 10ml in terms of ferrous sulfate, is 48.56mg.
Embodiment 3：
Melanterite 5.3g, sea-buckthorn 200g, hawthorn 100g, jujube 100g
Sea-buckthorn, hawthorn, three taste medicine of jujube is taken to add water to cook 2 times, for the first time plus 10 times of amounts of water decoct 1.5 hours, the second system
8 times of amounts of water are added to decoct 1 hour, collecting decoction filters, and filtrate is concentrated into every 1ml crude drugs containing 1g, lets cool, ethyl alcohol is added to make alcohol content
It is 70%, stirs evenly, stand overnight, leaching supernatant, filtrate recycling ethanol is spare to no alcohol taste；Melanterite adds 0.1% sulphur
Acid solution 50ml, stirs to dissolve, and vitamin C 5g is added in filtration, filtrate, is stirred to dissolve, solution for standby；Take sucrose
200g adds suitable quantity of water, and simple syrup is made, filtration, and above-mentioned two kinds of liquids are added, and mixing takes sodium hydroxide 0.3g, is added above-mentioned mixed
It closes in liquid, stirs evenly, pH value is adjusted to 2.5-4.0 with citric acid, is added to 1000ml, stirs evenly, stand, filtration is filling, goes out
Bacterium to get.
Ferrous sulfate reference substance 0.4g is taken, it is accurately weighed, it sets in 100ml measuring bottles, adds sulfuric acid solution (1 → 20) 1ml and water
80ml makes it dissolve, and adds water to scale, shakes up, and precision measures 2ml, sets in 100ml measuring bottles, is diluted with water to scale, shake up, i.e.,
Obtain reference substance solution；Precision measures reference substance solution 1.0ml, 2.0ml, 4.0ml, 6.0ml, 8.0ml, sets 25ml measuring bottles respectively
In, 10ml is added water to, then add 1% hydroxylamine hydrochloride solution 1ml and 0.2%2,2- bipyridyl 1ml, mixing adds water to scale, shakes
It is even, using corresponding solution as blank, according to UV-VIS spectrophotometry, absorbance is measured at 522nm wavelength, with absorbance
For ordinate, a concentration of abscissa draws standard curve；Precision measures this product 10ml, sets in 100ml measuring bottles, and instillation 1%~
2% sodium hydroxide solution is 3.5~4.5 to PH, adds water to scale, shakes up, and precision measures 5ml, sets in 25ml measuring bottles, adds water to
10ml, then add 1% hydroxylamine hydrochloride solution 1ml and 0.2%2,2- bipyridyl ethanol solution 1ml, mixing adds water to scale, shakes up,
Using corresponding solution as blank, absorbance is measured, from the weight for reading ferrous sulfate in test solution on standard curve, meter
Calculate to get.
This product contains melanterite per 10ml in terms of ferrous sulfate, is 49.15mg.
Embodiment 4
Melanterite 5.3g, sea-buckthorn 200g, hawthorn 100g, jujube 100g
Sea-buckthorn, hawthorn, three taste medicine of jujube is taken to add water to cook 2 times, for the first time plus 10 times of amounts of water decoct 1.5 hours, the second system
8 times of amounts of water are added to decoct 1 hour, collecting decoction filters, and filtrate is concentrated into every 1ml crude drugs containing 1g, lets cool, ethyl alcohol is added to make alcohol content
It is 70%, stirs evenly, stand overnight, leaching supernatant, filtrate recycling ethanol is spare to no alcohol taste；Melanterite adds 0.1% sulphur
Acid solution 50ml, stirs to dissolve, and vitamin C 5g is added in filtration, filtrate, is stirred to dissolve, solution for standby；Take sucrose
200g adds suitable quantity of water, and simple syrup is made, filtration, and above-mentioned two kinds of liquids are added, and mixing takes sodium hydroxide 0.6g, is added above-mentioned mixed
It closes in liquid, stirs evenly, pH value is adjusted to 2.5-4.0 with citric acid, is added to 1000ml, stirs evenly, stand, filtration is filling, goes out
Bacterium to get.
Ferrous sulfate reference substance 0.4g is taken, it is accurately weighed, it sets in 100ml measuring bottles, adds sulfuric acid solution (1 → 20) 1ml and water
80ml makes it dissolve, and adds water to scale, shakes up, and precision measures 2ml, sets in 100ml measuring bottles, is diluted with water to scale, shake up, i.e.,
Obtain reference substance solution；Precision measures reference substance solution 1.0ml, 2.0ml, 4.0ml, 6.0ml, 8.0ml, sets 25ml measuring bottles respectively
In, 10ml is added water to, then add 1% hydroxylamine hydrochloride solution 1ml and 0.2%2,2- bipyridyl 1ml, mixing adds water to scale, shakes
It is even, using corresponding solution as blank, according to UV-VIS spectrophotometry, absorbance is measured at 522nm wavelength, with absorbance
For ordinate, a concentration of abscissa draws standard curve；Precision measures this product 10ml, sets in 100ml measuring bottles, and instillation 1%~
2% sodium hydroxide solution is 3.5~4.5 to PH, adds water to scale, shakes up, and precision measures 5ml, sets in 25ml measuring bottles, adds water to
10ml, then add 1% hydroxylamine hydrochloride solution 1ml and 0.2%2,2- bipyridyl ethanol solution 1ml, mixing adds water to scale, shakes up,
Using corresponding solution as blank, absorbance is measured, from the weight for reading ferrous sulfate in test solution on standard curve, meter
Calculate to get.
This product contains melanterite per 10ml in terms of ferrous sulfate, is 47.38mg.
Embodiment 5：
Melanterite 4g, sea-buckthorn 180g, hawthorn 90g, jujube 120g
Sea-buckthorn, hawthorn, three taste medicine of jujube is taken to add water to cook 2 times, for the first time plus 10 times of amounts of water decoct 1.5 hours, second
8 times of amounts of water are added to decoct 1 hour, collecting decoction filters, and filtrate is concentrated into every 1ml crude drugs containing 1g, lets cool, ethyl alcohol is added to make alcohol content
It is 70%, stirs evenly, stand overnight, leaching supernatant, filtrate recycling ethanol is spare to no alcohol taste；Melanterite adds 0.1% sulphur
Acid solution 50ml, stirs to dissolve, and vitamin C 5g is added in filtration, filtrate, is stirred to dissolve, solution for standby；Take sucrose
200g adds suitable quantity of water, and simple syrup is made, and above-mentioned two kinds of liquids are added in filtration, and mixing takes sodium benzoate 2g, it is added above-mentioned mixed
It closes in liquid, stirs evenly, pH value is adjusted to 2.5-4.0 with citric acid, is added to 1000ml, stirs evenly, stand, filtration is filling, goes out
Bacterium to get.
Ferrous sulfate reference substance 0.4g is taken, it is accurately weighed, it sets in 100ml measuring bottles, adds sulfuric acid solution (1 → 20) 1ml and water
80ml makes it dissolve, and adds water to scale, shakes up, and precision measures 2ml, sets in 100ml measuring bottles, is diluted with water to scale, shake up, i.e.,
Obtain reference substance solution；Precision measures reference substance solution 1.0ml, 2.0ml, 4.0ml, 6.0ml, 8.0ml, sets 25ml measuring bottles respectively
In, 10ml is added water to, then add 1% hydroxylamine hydrochloride solution 1ml and 0.2%2,2- bipyridyl 1ml, mixing adds water to scale, shakes
It is even, using corresponding solution as blank, according to UV-VIS spectrophotometry, absorbance is measured at 522nm wavelength, with absorbance
For ordinate, a concentration of abscissa draws standard curve；Precision measures this product 10ml, sets in 100ml measuring bottles, and instillation 1%~
2% sodium hydroxide solution is 3.5~4.5 to PH, adds water to scale, shakes up, and precision measures 5ml, sets in 25ml measuring bottles, adds water to
10ml, then add 1% hydroxylamine hydrochloride solution 1ml and 0.2%2,2- bipyridyl ethanol solution 1ml, mixing adds water to scale, shakes up,
Using corresponding solution as blank, absorbance is measured, from the weight for reading ferrous sulfate in test solution on standard curve, meter
Calculate to get.
This product contains melanterite per 10ml in terms of ferrous sulfate, is 44.67mg.
Embodiment 6：
Melanterite 4g, sea-buckthorn 180g, hawthorn 90g, jujube 120g
Sea-buckthorn, hawthorn, three taste medicine of jujube is taken to add water to cook 2 times, for the first time plus 10 times of amounts of water decoct 1.5 hours, the second system
8 times of amounts of water are added to decoct 1 hour, collecting decoction filters, and filtrate is concentrated into every 1ml crude drugs containing 1g, lets cool, ethyl alcohol is added to make alcohol content
It is 70%, stirs evenly, stand overnight, leaching supernatant, filtrate recycling ethanol is spare to no alcohol taste；Melanterite adds 0.1% sulphur
Acid solution 50ml, stirs to dissolve, and vitamin C 5g is added in filtration, filtrate, is stirred to dissolve, solution for standby；Take sucrose
200g adds suitable quantity of water, and simple syrup is made, filtration, and above-mentioned two kinds of liquids are added, and mixing takes sodium hydroxide 0.56g, is added above-mentioned
It in admixing medical solutions, stirs evenly, pH value is adjusted to 2.5-4.0 with citric acid, is added to 1000ml, stirs evenly, stand, filtration is filling, goes out
Bacterium to get.
Ferrous sulfate reference substance 0.4g is taken, it is accurately weighed, it sets in 100ml measuring bottles, adds sulfuric acid solution (1 → 20) 1ml and water
80ml makes it dissolve, and adds water to scale, shakes up, and precision measures 2ml, sets in 100ml measuring bottles, is diluted with water to scale, shake up, i.e.,
Obtain reference substance solution；Precision measures reference substance solution 1.0ml, 2.0ml, 4.0ml, 6.0ml, 8.0ml, sets 25ml measuring bottles respectively
In, 10ml is added water to, then add 1% hydroxylamine hydrochloride solution 1ml and 0.2%2,2- bipyridyl 1ml, mixing adds water to scale, shakes
It is even, using corresponding solution as blank, according to UV-VIS spectrophotometry, absorbance is measured at 522nm wavelength, with absorbance
For ordinate, a concentration of abscissa draws standard curve；Precision measures this product 10ml, sets in 100ml measuring bottles, and instillation 1%~
2% sodium hydroxide solution is 3.5~4.5 to PH, adds water to scale, shakes up, and precision measures 5ml, sets in 25ml measuring bottles, adds water to
10ml, then add 1% hydroxylamine hydrochloride solution 1ml and 0.2%2,2- bipyridyl ethanol solution 1ml, mixing adds water to scale, shakes up,
Using corresponding solution as blank, absorbance is measured, from the weight for reading ferrous sulfate in test solution on standard curve, meter
Calculate to get.
This product contains melanterite per 10ml in terms of ferrous sulfate, is 44.78mg.
Embodiment 7：
Melanterite 4g, sea-buckthorn 180g, hawthorn 90g, jujube 120g
Sea-buckthorn, hawthorn, three taste medicine of jujube is taken to add water to cook 2 times, for the first time plus 10 times of amounts of water decoct 1.5 hours, the second system
8 times of amounts of water are added to decoct 1 hour, collecting decoction filters, and filtrate is concentrated into every 1ml crude drugs containing 1g, lets cool, ethyl alcohol is added to make alcohol content
It is 70%, stirs evenly, stand overnight, leaching supernatant, filtrate recycling ethanol is spare to no alcohol taste；Melanterite adds 0.1% sulphur
Acid solution 50ml, stirs to dissolve, and vitamin C 5g is added in filtration, filtrate, is stirred to dissolve, solution for standby；Take sucrose
200g adds suitable quantity of water, and simple syrup is made, filtration, and above-mentioned two kinds of liquids are added, and mixing takes sodium hydroxide 0.3g, is added above-mentioned mixed
It closes in liquid, stirs evenly, pH value is adjusted to 2.5-4.0 with citric acid, is added to 1000ml, stirs evenly, stand, filtration is filling, goes out
Bacterium to get.
Ferrous sulfate reference substance 0.4g is taken, it is accurately weighed, it sets in 100ml measuring bottles, adds sulfuric acid solution (1 → 20) 1ml and water
80ml makes it dissolve, and adds water to scale, shakes up, and precision measures 2ml, sets in 100ml measuring bottles, is diluted with water to scale, shake up, i.e.,
Obtain reference substance solution；Precision measures reference substance solution 1.0ml, 2.0ml, 4.0ml, 6.0ml, 8.0ml, sets 25ml measuring bottles respectively
In, 10ml is added water to, then add 1% hydroxylamine hydrochloride solution 1ml and 0.2%2,2- bipyridyl 1ml, mixing adds water to scale, shakes
It is even, using corresponding solution as blank, according to UV-VIS spectrophotometry, absorbance is measured at 522nm wavelength, with absorbance
For ordinate, a concentration of abscissa draws standard curve；Precision measures this product 10ml, sets in 100ml measuring bottles, and instillation 1%~
2% sodium hydroxide solution is 3.5~4.5 to PH, adds water to scale, shakes up, and precision measures 5ml, sets in 25ml measuring bottles, adds water to
10ml, then add 1% hydroxylamine hydrochloride solution 1ml and 0.2%2,2- bipyridyl ethanol solution 1ml, mixing adds water to scale, shakes up,
Using corresponding solution as blank, absorbance is measured, from the weight for reading ferrous sulfate in test solution on standard curve, meter
Calculate to get.
This product contains melanterite per 10ml in terms of ferrous sulfate, is 44.46mg.
Embodiment 8
Melanterite 6g, sea-buckthorn 210g, hawthorn 110g, jujube 100g
Sea-buckthorn, hawthorn, three taste medicine of jujube is taken to add water to cook 2 times, for the first time plus 10 times of amounts of water decoct 1.5 hours, the second system
8 times of amounts of water are added to decoct 1 hour, collecting decoction filters, and filtrate is concentrated into every 1ml crude drugs containing 1g, lets cool, ethyl alcohol is added to make alcohol content
It is 70%, stirs evenly, stand overnight, leaching supernatant, filtrate recycling ethanol is spare to no alcohol taste；Melanterite adds 0.1% sulphur
Acid solution 50ml, stirs to dissolve, and vitamin C 5g is added in filtration, filtrate, is stirred to dissolve, solution for standby；Take sucrose
200g adds suitable quantity of water, and simple syrup is made, filtration, and above-mentioned two kinds of liquids are added, and mixing takes sodium hydroxide 0.6g, is added above-mentioned mixed
It closes in liquid, stirs evenly, pH value is adjusted to 2.5-4.0 with citric acid, is added to 1000ml, stirs evenly, stand, filtration is filling, goes out
Bacterium to get.
Ferrous sulfate reference substance 0.4g is taken, it is accurately weighed, it sets in 100ml measuring bottles, adds sulfuric acid solution (1 → 20) 1ml and water
80ml makes it dissolve, and adds water to scale, shakes up, and precision measures 2ml, sets in 100ml measuring bottles, is diluted with water to scale, shake up, i.e.,
Obtain reference substance solution；Precision measures reference substance solution 1.0ml, 2.0ml, 4.0ml, 6.0ml, 8.0ml, sets 25ml measuring bottles respectively
In, 10ml is added water to, then add 1% hydroxylamine hydrochloride solution 1ml and 0.2%2,2- bipyridyl 1ml, mixing adds water to scale, shakes
It is even, using corresponding solution as blank, according to UV-VIS spectrophotometry, absorbance is measured at 522nm wavelength, with absorbance
For ordinate, a concentration of abscissa draws standard curve；Precision measures this product 10ml, sets in 100ml measuring bottles, and instillation 1%~
2% sodium hydroxide solution is 3.5~4.5 to PH, adds water to scale, shakes up, and precision measures 5ml, sets in 25ml measuring bottles, adds water to
10ml, then add 1% hydroxylamine hydrochloride solution 1ml and 0.2%2,2- bipyridyl ethanol solution 1ml, mixing adds water to scale, shakes up,
Using corresponding solution as blank, absorbance is measured, from the weight for reading ferrous sulfate in test solution on standard curve, meter
Calculate to get.
This product contains melanterite per 10ml in terms of ferrous sulfate, is 45.06mg.
Embodiment 9：
Melanterite 6g, sea-buckthorn 210g, hawthorn 110g, jujube 100g
Sea-buckthorn, hawthorn, three taste medicine of jujube is taken to add water to cook 2 times, for the first time plus 10 times of amounts of water decoct 1.5 hours, the second system
8 times of amounts of water are added to decoct 1 hour, collecting decoction filters, and filtrate is concentrated into every 1ml crude drugs containing 1g, lets cool, ethyl alcohol is added to make alcohol content
It is 70%, stirs evenly, stand overnight, leaching supernatant, filtrate recycling ethanol is spare to no alcohol taste；Melanterite adds 0.1% sulphur
Acid solution 50ml, stirs to dissolve, and vitamin C 5g is added in filtration, filtrate, is stirred to dissolve, solution for standby；Take sucrose
200g adds suitable quantity of water, and simple syrup is made, filtration, and above-mentioned two kinds of liquids are added, and mixing takes sodium hydroxide 0.3g, is added above-mentioned mixed
It closes in liquid, stirs evenly, pH value is adjusted to 2.5-4.0 with citric acid, is added to 1000ml, stirs evenly, stand, filtration is filling, goes out
Bacterium to get.
Ferrous sulfate reference substance 0.4g is taken, it is accurately weighed, it sets in 100ml measuring bottles, adds sulfuric acid solution (1 → 20) 1ml and water
80ml makes it dissolve, and adds water to scale, shakes up, and precision measures 2ml, sets in 100ml measuring bottles, is diluted with water to scale, shake up, i.e.,
Obtain reference substance solution；Precision measures reference substance solution 1.0ml, 2.0ml, 4.0ml, 6.0ml, 8.0ml, sets 25ml measuring bottles respectively
In, 10ml is added water to, then add 1% hydroxylamine hydrochloride solution 1ml and 0.2%2,2- bipyridyl 1ml, mixing adds water to scale, shakes
It is even, using corresponding solution as blank, according to UV-VIS spectrophotometry, absorbance is measured at 522nm wavelength, with absorbance
For ordinate, a concentration of abscissa draws standard curve；Precision measures this product 10ml, sets in 100ml measuring bottles, and instillation 1%~
2% sodium hydroxide solution is 3.5~4.5 to PH, adds water to scale, shakes up, and precision measures 5ml, sets in 25ml measuring bottles, adds water to
10ml, then add 1% hydroxylamine hydrochloride solution 1ml and 0.2%2,2- bipyridyl ethanol solution 1ml, mixing adds water to scale, shakes up,
Using corresponding solution as blank, absorbance is measured, from the weight for reading ferrous sulfate in test solution on standard curve, meter
Calculate to get.
This product contains melanterite per 10ml in terms of ferrous sulfate, is 51.23mg.
Embodiment 10
Melanterite 4g, sea-buckthorn 180g, hawthorn 90g, jujube 120g
Sea-buckthorn, hawthorn, three taste medicine of jujube is taken to add water to cook 2 times, for the first time plus 10 times of amounts of water decoct 1.5 hours, the second system
8 times of amounts of water are added to decoct 1 hour, collecting decoction filters, and filtrate is concentrated into every 1ml crude drugs containing 1g, lets cool, ethyl alcohol is added to make alcohol content
It is 70%, stirs evenly, stand overnight, leaching supernatant, filtrate recycling ethanol is spare to no alcohol taste；Melanterite adds 0.1% sulphur
Acid solution 50ml, stirs to dissolve, and vitamin C 5g is added in filtration, filtrate, is stirred to dissolve, solution for standby；Take sucrose
200g adds suitable quantity of water, and simple syrup is made, filtration, and above-mentioned two kinds of liquids are added, and mixing takes sodium hydroxide 0.6g, is added above-mentioned mixed
It closes in liquid, stirs evenly, pH value is adjusted to 2.5-4.0 with citric acid, is added to 1000ml, stirs evenly, stand, filtration is filling, goes out
Bacterium to get.
Ferrous sulfate reference substance 0.4g is taken, it is accurately weighed, it sets in 100ml measuring bottles, adds sulfuric acid solution (1 → 20) 1ml and water
80ml makes it dissolve, and adds water to scale, shakes up, and precision measures 2ml, sets in 100ml measuring bottles, is diluted with water to scale, shake up, i.e.,
Obtain reference substance solution；Precision measures reference substance solution 1.0ml, 2.0ml, 4.0ml, 6.0ml, 8.0ml, sets 25ml measuring bottles respectively
In, 10ml is added water to, then add 1% hydroxylamine hydrochloride solution 1ml and 0.2%2,2- bipyridyl 1ml, mixing adds water to scale, shakes
It is even, using corresponding solution as blank, according to UV-VIS spectrophotometry, absorbance is measured at 522nm wavelength, with absorbance
For ordinate, a concentration of abscissa draws standard curve；Precision measures this product 10ml, sets in 100ml measuring bottles, and instillation 1%~
2% sodium hydroxide solution is 3.5~4.5 to PH, adds water to scale, shakes up, and precision measures 5ml, sets in 25ml measuring bottles, adds water to
10ml, then add 1% hydroxylamine hydrochloride solution 1ml and 0.2%2,2- bipyridyl ethanol solution 1ml, mixing adds water to scale, shakes up,
Using corresponding solution as blank, absorbance is measured, from the weight for reading ferrous sulfate in test solution on standard curve, meter
Calculate to get.
This product contains melanterite per 10ml in terms of ferrous sulfate, is 50.89mg.
Claims (6)
1. a kind of for postpartum ischemic, the detection method of content of the Chinese medicine composition of lochia, it is characterised in that contain in this method
Following content assaying method：Take ferrous sulfate reference substance 0.4g, it is accurately weighed, set in 100ml measuring bottles, add sulfuric acid solution (1 →
20) 1ml and water 80ml make it dissolve, and add water to scale, shake up, and precision measures 2ml, sets in 100ml measuring bottles, is diluted with water to quarter
Degree, shakes up to get reference substance solution；
Precision measures reference substance solution 1.0ml, 2.0ml, 4.0ml, 6.0ml, 8.0ml, sets in 25ml measuring bottles, adds water to respectively
10ml, then add 1% hydroxylamine hydrochloride solution 1ml and 0.2%2,2- bipyridyl 1ml, mixing adds water to scale, shakes up, with corresponding
Solution is blank, and according to UV-VIS spectrophotometry, absorbance is measured at 522nm wavelength, dense using absorbance as ordinate
Degree is abscissa, draws standard curve；
Precision measures this product 10ml, sets in 100ml measuring bottles, and it is 3.5~4.5 to instill 1%~2% sodium hydroxide solution to PH, is added
Water shakes up to scale, and precision measures 5ml, sets in 25ml measuring bottles, adds water to 10ml, then add 1% hydroxylamine hydrochloride solution 1ml and
0.2%2,2- bipyridyl ethanol solution 1ml, mixing adds water to scale, shakes up, and using corresponding solution as blank, measures extinction
Degree, from standard curve read test solution in ferrous sulfate weight, calculate to get；
It is signified in this method to be for postpartum ischemic, the bulk pharmaceutical chemicals of the Chinese medicine composition of lochia and proportioning：2~8 weight of melanterite
Part, 180~220 parts by weight of sea-buckthorn, 80~120 parts by weight of hawthorn, 80~120 parts by weight of jujube.
2. the detection method of content of Chinese medicine composition as described in claim 1, it is characterised in that signified Chinese medicine in this method
The bulk pharmaceutical chemicals group of composite preparation becomes：5.3 parts by weight of melanterite, 200 parts by weight of sea-buckthorn, 100 parts by weight of hawthorn, 100 weight of jujube
Measure part.
3. the detection method of content of Chinese medicine composition as described in claim 1, it is characterised in that signified Chinese medicine in this method
Composite preparation is made by the following method：Sea-buckthorn, hawthorn, three taste medicine of jujube is taken to add water to cook 2 times, for the first time plus 10 times of amounts of water are decocted
It boils 1.5 hours, the second system adds 8 times of amounts of water to decoct 1 hour, collecting decoction, filtration, and filtrate is concentrated into every 1ml crude drugs containing 1g, puts
It is cold, add ethyl alcohol that alcohol content is made to be 70%, stir evenly, stand overnight, leaching supernatant, filtrate recycling ethanol is standby to no alcohol taste
With；Melanterite adds 0.1% sulfuric acid solution 50ml, stirs to dissolve, and vitamin C 5g is added in filtration, filtrate, is stirred to dissolve,
Solution for standby；Sucrose 200g is taken, suitable quantity of water is added, simple syrup is made, is filtered, above-mentioned two kinds of liquids are added, mixing takes sodium hydroxide
0.2~0.8g is added in above-mentioned admixing medical solutions, stirs evenly, and adjusts pH value to 2.5-4.0 with citric acid, is added to 1000ml, stirs
It is even, stand, filtration, it is filling, sterilizing to get.
4. the detection method of content of Chinese medicine composition as claimed in claim 3, it is characterised in that signified Chinese medicine in this method
Composite preparation is made by the following method：
Sea-buckthorn, hawthorn, three taste medicine of jujube is taken to add water to cook 2 times, for the first time plus 10 times of amounts of water decoct 1.5 hours, second plus water 8
Times amount decocts 1 hour, and collecting decoction, filtration, filtrate is concentrated into every 1ml crude drugs containing 1g, lets cool, and ethyl alcohol is added to make the alcohol content be
70%, it stirs evenly, stands overnight, leaching supernatant, filtrate recycling ethanol is spare to no alcohol taste；Melanterite adds 0.1% sulfuric acid
Solution 50ml, stirs to dissolve, and vitamin C 5g is added in filtration, filtrate, is stirred to dissolve, solution for standby；Sucrose 200g is taken,
Add suitable quantity of water, simple syrup is made, filter, above-mentioned two kinds of liquids are added, mixing takes sodium hydroxide 0.56g, above-mentioned mixing medicine is added
It in liquid, stirs evenly, pH value is adjusted to 2.5-4.0 with citric acid, is added to 1000ml, stirs evenly, stand, filtration is filling, sterilizing, i.e.,
.
5. the detection method of content of Chinese medicine composition as described in claim 1, it is characterised in that signified Chinese medicine in this method
Composite preparation is made by the following method：
Sea-buckthorn, hawthorn, three taste medicine of jujube is taken to add water to cook 2 times, for the first time plus 10 times of amounts of water decoct 1.5 hours, second plus water 8
Times amount decocts 1 hour, and collecting decoction, filtration, filtrate is concentrated into every 1ml crude drugs containing 1g, lets cool, and ethyl alcohol is added to make the alcohol content be
70%, it stirs evenly, stands overnight, leaching supernatant, filtrate recycling ethanol is spare to no alcohol taste；Melanterite adds 0.1% sulfuric acid
Solution 50ml, stirs to dissolve, and vitamin C 5g is added in filtration, filtrate, is stirred to dissolve, solution for standby；Sucrose 200g is taken,
Add suitable quantity of water, simple syrup is made, filter, above-mentioned two kinds of liquids are added, mixing takes sodium benzoate 2g, above-mentioned admixing medical solutions are added
In, stir evenly, pH value adjusted to 2.5-4.0 with citric acid, is added to 1000ml, stirs evenly, stand, filtration is filling, sterilizing to get.
6. the detection method of content of Chinese medicine composition described in claim 1, it is characterised in that：This product is per 10ml containing melanterite with sulphur
Sour ferrous meter, must not be less than 45mg.
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| CN101077372A (en) * | 2007-03-07 | 2007-11-28 | 郑光明 | Chinese medicinal materials composition for preventing and treating iron-deficiency anemia and method of making the same |
| CN102309572A (en) * | 2010-07-02 | 2012-01-11 | 程雪翔 | Blood nourishing oral liquid and preparation method thereof |
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| CN101077372A (en) * | 2007-03-07 | 2007-11-28 | 郑光明 | Chinese medicinal materials composition for preventing and treating iron-deficiency anemia and method of making the same |
| CN102309572A (en) * | 2010-07-02 | 2012-01-11 | 程雪翔 | Blood nourishing oral liquid and preparation method thereof |
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