Summary of the invention
The object of the present invention is to provide a kind of pre-mixing agent and the application thereof that increase weanling pig colon SCFA, to increase colon SCFA, keep the integrality of colon function.
In order to reach above-mentioned purpose, solution of the present invention is:
Increase a pre-mixing agent for weanling pig colon SCFA, by following material by weight proportioning form:
Bacillus acidi lactici 50-150,
Glutamine 5-10,
Hawthorn 20-50,
Sodium glutamate 2-10,
Arginine 5-10,
Pomace 300-500,
System chaff 270-618.
Bacillus acidi lactici can improve the microorganism species of enteron aisle, and glutamine, sodium glutamate, arginine can provide gut flora nutrition, are degraded to SCFA simultaneously, and pomace is good dietary fiber, can be utilized at enteron aisle by microorganism.
The present invention is applied in when healthy weanling pig is weaned in basal diet, and by weight percentage, adding proportion is 1%.
After adopting such scheme, Bacillus acidi lactici of the present invention directly acts on the microorganism of enteron aisle, regulate the kind group relation of microorganism, glutamine, sodium glutamate and arginine are utilized by the microorganism of enteron aisle after can degrading, there is provided the energy source of microorganism, the substrate that pomace mainly provides enteric microorganism to grow.The present invention can increase colon SCFA, keeps the integrality of colon function.
Detailed description of the invention
Testing program:
Choose the healthy weanling pig of 120 DLYs, body weight 7.0 ± 0.11kg, is divided into 4 groups at random, adds respectively in the table 1 of 1% respectively organize 1, group 2, group 3, the pre-mixing agent of group 4 when weaning on basal diet.Feeding time 10 days, after feeding 10 days, the bloodletting of piglet heart is lethal, and cut abdominal cavity open and gather colon chyme, it is for subsequent use that then liquid nitrogen flash freezer proceeds to-80 DEG C of Refrigerator stores.Claim during the nascent and wean of piglet and body weight at the end of testing.
Colon fatty acid determination: adopt Otto(2003) method, and suitably to adjust.Get 2g to thaw chyme sample, use 8mL distilled water diluting, add 2 concentrated hydrochloric acid mixings, 4 DEG C 17, the centrifugal 10min of 400 × g, with 0.45 μm of filtration, get 1mL filtered fluid, to add in adsorbent (anhydrous Na 2SO4:50%H2SO4: diatomite=30:1:20) in the test tube of 10mL, add 3mL chloroform, acutely sway immediately, leave standstill 10 ~ 15min, get supernatant gas Chromatographic Determination.Nitrogen speed is 25mL/min, and air and hydrogen are pilot gas, flow velocity 30mL/min, each Sample Injection 5 μ L.Acetic acid in titer, propionic acid, butyric acid, isobutyric acid, valeric acid, isovaleric acid concentration is respectively 1.36,1.055,0.885,0.84,0.705,0.705mM.
Table 1 pre-mixing agent composition (g/Kg)
Composition |
Group 1 |
Group 2 |
Group 3 |
Group 4 |
Bacillus acidi lactici |
0 |
50 |
100 |
150 |
Glutamine |
0 |
5 |
8 |
10 |
Hawthorn |
0 |
20 |
30 |
50 |
Sodium glutamate |
0 |
2 |
5 |
10 |
Arginine |
0 |
5 |
10 |
10 |
Pomace |
0 |
300 |
500 |
500 |
System chaff |
1000 |
618 |
347 |
270 |
Described Bacillus acidi lactici is viable count is 8,000,000,000 CFU/g.
Bacillus acidi lactici can improve the microorganism species of enteron aisle, glutamine, sodium glutamate, and arginine can provide gut flora nutrition, is degraded to SCFA simultaneously, and pomace is good dietary fiber, can be utilized at enteron aisle by microorganism.
Pre-mixing agent preparation method:
Hawthorn pulverized 40 orders, and apple removes residue after squeezing the juice, and hawthorn, glutamine, sodium glutamate, arginine and system chaff mix.
Result of the test:
The ability (mg/g) of table 2 pre-mixing agent scavenging free radicals
Group |
Group 1 |
Group 2 |
Group 3 |
Group 4 |
Weaning weight (24 days, kg) |
7.10 |
6.85 |
7.05 |
7.19 |
Body weight (kg) at the end of experiment |
9.85 |
10.26 |
10.55 |
10.25 |
Acetic acid |
0.23 |
0.27 |
0.35 |
0.26 |
Propionic acid |
0.76 |
1.02 |
1.28 |
0.98 |
Isobutyric acid |
0.15 |
0.18 |
0.25 |
0.16 |
Butyric acid |
0.29 |
0.42 |
0.56 |
0.38 |
Isovaleric acid |
0.20 |
0.27 |
0.35 |
0.32 |
Valeric acid |
0.16 |
0.26 |
0.35 |
0.35 |
As can be seen from Table 2, at the end of the experiment of group 3, body weight is maximum, and reach 10.55kg, comparatively control group adds 0.7kg/ head.And acetic acid in group 3 in chyme, propionic acid, isobutyric acid, butyric acid, isovaleric acid, valeric acid concentration the highest, reach 0.35mg/g respectively, 1.28mg/g, 0.25mg/g, 0.56mg/g, 0.35mg/g, 0.35mg/g, be significantly higher than control group.
Above-described embodiment non-limiting the present invention, any person of an ordinary skill in the technical field, to its suitable change done or modification, all should be considered as not departing from patent category of the present invention.