CN104894102A - Ultrasonic reinforcement culture method of microalgae - Google Patents
Ultrasonic reinforcement culture method of microalgae Download PDFInfo
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Abstract
The invention provides an ultrasonic reinforcement culture method of microalgae. The method includes selection and cleaning of a certain amount of algae, production of a microalgae solid medium, and conservation and spread culture of microalgae species in laboratory. The method is as below: first selecting a liquid medium appropriate for microalgae growth, and adding gel to obtain a solid medium; selecting sterile purified microalgae species, inoculating into the solid medium, sealing and culturing; and finally conducting initial expansion culture and relaying culture of microalgae species; and carrying out quality control on microalgae species, and screening out a high-quality microalgae species. Through ultrasonic reinforcement on high-quality microalgae species, cell wall partial rupture caused by ultrasonic improves the permeability of cell membrane, thus promoting the release of metabolites, promoting cell growth and metabolism, and increasing growth rate.
Description
Technical field
The invention belongs to microdisk electrode production technical field, be specifically related to a kind of method that can be used for the ultrasound-enhanced microdisk electrode producing bioenergy, biomaterial, food, feed, healthcare products and medicine.
Background technology
Micro-algae is as the Biological resources of preciousness, and a major reason of not also being fully used so far is exactly do not have economic production system.At present, method for microdisk electrode has racetrack culture method, bioreactor culture method etc., such as, but the racetrack cultivation pool needed for racetrack culture method only can cultivate a few algae kind grown in selective medium, micro-algae kind such as spirulina and salt algae; And the photo-bioreactor system closed used in bioreactor culture method can cultivate most micro-algae kind, but laid down cost and running cost are very high.This situation limits the development of micro-algae industry to a great extent.
Runway recognition pond initial design is in the sixties in last century, current Technical comparing is ripe, be widely used in the cultivation of spirulina, chlorella, Dunaliella salina and Haematocoocus Pluvialls, but, due to the cultivation facility that racetrack cultivation pool is an opening, the micro-algae wherein cultivated is easy to be subject to the pollution of alien species; Meanwhile, the culture condition such as the temperature of cultivation pool are difficult to control, and can not provide desirable culture condition for micro-algae.The culture condition that bioreactor can provide closed culture environment and can regulate and control, most micro-algae kinds can be cultivated in theory better, it is the emphasis of industry research and development for many years, but at present also there is construction and the high problem of use cost in bioreactor, be only applicable to the production of the micro-algae product of high added value, the application in micro-algae industry has certain limitation.
The design of current bioreactor and construction are one of difficult points of technical field of microalga biology.Although through researchdevelopment for many years, various types of reactor is designed and builds, and is even used to commercially produce micro-algae product, and never mature and stable on a large scale cheap bioreactor facility is sold.The bottleneck of micro-algae industry development that do not had suitable photo-bioreactor system to become.The culture condition that bioreactor can provide closed culture environment and can regulate and control, most micro-algae kinds can be cultivated in theory better, it is the emphasis of industry research and development for many years, but at present also there is construction and the high problem of use cost in bioreactor, be only applicable to the production of the micro-algae product of high added value, application in micro-algae industry has certain limitation, and the method for batch microdisk electrode does not also form the operational path of a whole set of maturation.
Summary of the invention
For this reason, the present invention proposes a kind of method of ultrasound-enhanced microdisk electrode, the method adopts bioreactor and racetrack cultivation pool to combine the technology of carrying out microdisk electrode, can effectively reduce microdisk electrode cost, and define the operational path of the micro-algae of a whole set of complete cultivation.
A method for ultrasound-enhanced microdisk electrode, comprises the following steps:
A, select and clean a certain amount of algae, make micro-algae solid medium, the laboratory conservation expanding species of micro-algae is cultivated, first the liquid nutrient medium that suitable micro algae growth needs is chosen, add the sepharose of 1% wherein, obtain solid medium, solid medium is inserted in incubator and cultivates, culture temperature 20 DEG C, intensity of illumination 30uE/m
2/ s;
B, to choose through the aseptic micro-algae algae kind of purifying, be inoculated in described solid medium, sealing is cultivated; Last initial enlargement of carrying out micro-algae algae kind is successively cultivated, relaying is cultivated, and carries out quality control, filter out high-quality micro-algae algae kind after relaying is cultivated to micro-algae algae kind;
C, step b gained high-quality micro-algae algae kind is put into outdoor optical bio-reactor, open reactor control unit, set temperature controls at 25 DEG C, pH 7.4, air flow 25L/min; Carry out supersound process again after having reacted, ultrasonic power is 500 ~ 3000W/m
2, and carry out supersound process, obtain micro-algae plantation liquid;
D, micro-for step c gained algae plantation liquid is inoculated into outdoor racetrack cultivation pool, in racetrack cultivation pool, carry out extensive microdisk electrode, until when algae liquid becomes scarlet, collect whole pond algae liquid, pH in described outdoor racetrack cultivation pool is 8.0, and incubation time is 8 days;
E, meet d, downstream processing is carried out to whole pond algae liquid, is separated, frustule of gathering.
Preferably, micro-for high-quality algae algae kind is put into outdoor gas lift duct type bioreactor and cultivates, control temperature is 12-28 DEG C, pH 7.0-8.0, and air flow, at 100L/min, cultivates 5-7 days.
The Advantageous Effects that the present invention brings:
The present invention is by carrying out supersound process to high-quality micro-algae algae kind, the cell walls partial fracture that ultrasonic wave causes can improve membrane passage, thus promote the effects such as the release of meta-bolites, to promote growth and the metabolism of cell, improve the speed of growth, promote Metabolite Accumulation, shorten culture cycle.
Carry out the content that supersound process can not reduce grease in microalgae cell or hydrocarbon in culturing process of the present invention, thus can not have an impact to the economic worth of energy microalgae;
Outdoor optical bio-reactor and racetrack cultivation pool are combined, the method can large-scale culturing micro-algae, compare simple racetrack reaction tank and micro-algae cultivated by simple photoproduction reaction device, present invention significantly reduces production cost, and develop a whole set of complete operational path.
Embodiment
Selected by the present invention, raw material all obtains by commercial channel.
The present invention, a kind of method of ultrasound-enhanced microdisk electrode, specifically comprises the following steps:
The laboratory enlarged culturing of the first step, micro-algae
1, micro-algae conservation solid medium is prepared
According to the growth needs of micro-algae, select suitable liquid nutrient medium, add sepharose, utilize sterile petri dish to make sterile solid substratum after high-temperature sterilization on aseptic working platform, dropping to after air-setting after with sealed membrane sealing in temperature until substratum temperature is 4 DEG C of preservations.
2, the preservation of micro-algae algae kind
The micro-algae algae kind aseptic through purifying is inoculated in aseptic operating platform conservation on solid medium that above-mentioned steps 1 makes to cultivate, cultivation can in the incubator that lighting source is housed, when needing conservation to cultivate, the culture dish being loaded with micro-algae algae kind is moved on to aseptic operating platform, the micro-algae of transfer is signed to blank solid medium culture dish with aseptic, then cultivate with being put into incubator again after sealed membrane sealing, the timed interval that conservation is cultivated can be determined according to micro algae growth speed.
3, the initial enlargement of algae kind is cultivated
Configuration liquid nutrient medium, be dispensed into respectively in large, medium and small three class triangle culturing bottles, the liquid volume of packing is approximately 10-20mL, 100-300mL and 500-1000mL, and after being sealed by triangular flask with aluminium foil and tampon, high-temperature sterilization is stand-by; The initial expanding species the first step in aseptic operating platform with aseptic cotton carrier by micro-algae from solid culture group-transfer a little to little triangle culturing bottle, cultivate in the shaking table incubator having light source; Through arriving the cultivation in several week in a few days, after nutrient solution darkens, then transfer to middle culturing bottle through aseptic technique and large culturing bottle is cultivated.
4, the relaying of algae kind is cultivated
Configuration liquid nutrient medium is in the transparent drum of 10L or 20L to capacity, high-temperature sterilization is stand-by, algae liquid in large culturing bottle is transferred in the cultivation bucket after sterilizing through aseptic technique and cultivates, the aseptic air being mixed with carbonic acid gas is passed in bucket, appropriate illumination is provided, control the temperature in culturing room, for the algae being easy to sedimentation, assist and prevent algae sedimentation with shaking table facility.
5, the quality control of algae kind expansion process
In algae seedling solution body enlarged culturing process, the quality controlling micro-algae seedling solution be paid close attention to.Can by the quality of outward appearance and microscopic examination qualification algae kind, visual inspection be see algae liquid color whether normally, whether there is the phenomenon of precipitation and attached wall; Concerning economic micro-algae of speaking more greatly, precipitation and attached wall are abnormal, algae kind poor growth is described, algae kind should be abandoned and high-temperature inactivation, microscopic examination is whether observe the microscopic pattern of micro-algae normal, whether there are external algae or microbial contamination, once find that algae kind is polluted, algae liquid should be abandoned immediately and do high-temperature inactivation process.
The bioreactor of second step, micro-algae is cultivated
1, the preparation of bioreactor
Bioreactor, before inoculation, carries out cleaning and sterilizing; The liquid comes into contact part of reactor can carry out disinfection with chemical reagent sodium hypochlorite solution or potassium permanganate solution etc., and the gaseous decontaminant sterilizations such as the gas piping ozone of reactor or nitrogen peroxide, after sterilization, pass into sterile water wash; Finally connect the monitor and forecast equipment of reactor, pass into the air of filtration sterilization, add the nutrient chemical of filtration sterilization, the monitoring and control equipment of verification reactor.
2, the inoculation of small-scale bioreactor
By several barrels in the ready bioreactor of the aseptic importing of algae kind of quality inspection, observe and record the monitoring result of reactor and the validity of operating device, setting party answers the controling parameters of device.
3, micro-algae enlarged culturing of bioreactor
Postvaccinal small-scale reactor is after cultivation in a few days, reach suitable cultivation concentration, just can shift algae liquid to cultivate to commercial scale reactor, before the transfer of algae liquid, to first get out large-scale reactor, transfer process adopts aseptic technique, ensures that algae liquid is not subject to external biological pollution, and the small-scale reactor cleaning after transfer is for subsequent use.
4, micro-algae Semi-continuous cultivation of bioreactor
Large-scale reactor, after cultivation in a few days, reaches the highest level of micro-algae throughput, at this time carries out ultrasonic cultivation, and the cultivation of micro-algae in bioreactor can adopt semi-continuous mode.
The racetrack cultivation pool of the 3rd step, micro-algae is cultivated
1, racetrack cultivation pool prepares
Racetrack cultivation pool is cleaned thoroughly before inoculation and sterilizes,
First rinsed by cultivation pool water pipe, remove the residues such as residual algae liquid, then pass into tap water to expiring pond, the runner in Primary culture pond, adds a certain amount of sterilizing agent as the sterilization such as sodium hypochlorite solution or potassium permanganate; After sterilization, again rinse cultivation pool with the tap water of filtration sterilization; The last tap water again passing into filtration sterilization, starts cultivation pool runner, adds the inorganic salts ingredients of substratum, the monitor and forecast equipment in Primary culture pond.Here need control parameter time the degree of depth of substratum, the rotating speed pH etc. of cultivation pool runner.
2, racetrack cultivation pool inoculation
Algae liquid at commercial scale reactor Semi-continuous cultivation is transferred to cultivation pool, the culture condition of micro-algae liquid is controlled in transfer process, ensure the health of micro-algae in transfer process, one of parameter that racetrack cultivation pool inoculation needs control is the inoculum density of cultivation pool, and the production efficiency of micro-algae pond inoculum density to microdisk electrode has a great impact.
3, racetrack cultivation pool microdisk electrode
Micro-algae maintains certain culture condition in the cultivation of cultivation pool, controllable parameter comprises the rotating speed and pH etc. of runner, the removal of the influence of rotation speed dissolved oxygen of runner and effective stirring of nutrient solution, the cultivation of micro-algae in cultivation pool can be batch experiments or Semi-continuous cultivation.
4th step, the gathering and Product processing of micro-algae
1, micro-algae gathering and concentrated
Microalgae recovery can adopt various ways with concentrated, as natural subsidence, and centrifugal settling, flocculation and filtration etc., natural subsidence can be carried out in cultivation pool, also can transfer to special settling bowl and carry out, after primary settling, the equipment such as whizzer are utilized to proceed to concentrate to algae slurries.
2, the downstream processing of micro-algae
Micro-algae after concentrated can dry algae powder product or continue to extract valuable biological product or without drying program, extract biological products in advance from wet algal biomass.
Be described further below in conjunction with specific embodiment:
Embodiment 1:
The present invention, a kind of method of ultrasound-enhanced microdisk electrode, it specifically comprises the following steps:
The laboratory enlarged culturing of step 1, Haematocoocus Pluvialls
First the present invention preferably selects the BBM culture medium prescription of suitable micro algae growth to configure micro-algae liquid nutrient medium, and the sepharose adding 1% in liquid culture makes solid medium;
Secondly, in incubator, conservation cultivates micro-algae, culture temperature 20 DEG C, the about 30uE/m of intensity of illumination
2/ s;
Then, from the micro-algae of solid culture group-transfer to the 200ml culturing bottle that 20mL substratum is housed, in sculling incubator, cultivate about 14-21 days, culture temperature 20 DEG C, the about 30uE/m of intensity of illumination
2/ s, shaking table is about 40rpm;
Finally, select algae liquid color and turn green culturing bottle, visual inspection algae liquid does not precipitate and attached wall phenomenon, proceed to the 1L culturing bottle that 200mL substratum is housed to cultivate, whether whether microscopic examination algae liquid is healthy, pollute, if algae liquid pollutes, then abandon the algae kind enlarged culturing of this bottle, 1L culturing bottle is cultivated with the culture condition being same as 200mL culturing bottle.After 7-14 days, will turn green algae kind and expand to the cultivation culture in glassware of the 5L containing 1L substratum, culture condition is: culture temperature 20 DEG C, the about 80uE/m of intensity of illumination
2/ s, shaking table is about 30rpm, and algae kind is at the cultivation culture in glassware of 5L after about 7 days, and the capacity of proceeding to is the interior cultivation of transparent drum of 20L, and its culture condition is: culture temperature 25 DEG C, the about 100uE/m of intensity of illumination
2/ s, ventilation rate 0.2v/v/min, the gas passed into is containing 3%CO
2, after cultivating about 7 days, sampling check algae liquid quality and concentration, the algae liquid that healthy algae liquid concentration reaches 0.3g/L can be cultivated as algae kind inoculation bioreactor.
The bioreactor of step 2, Haematocoocus Pluvialls is cultivated
According to pilot scale needs, two kinds of outdoor gas lift duct types of 1000L and 8000L have been built in the present invention, ultrasonic cultivation is carried out in bioreactor, first according to the method described above cleaning and sterilizing is carried out to the bioreactor of 1000L, liquid disinfectant utilizes the sodium hypochlorite solution of 50ppm available chlorine, the ozone gas of gaseous decontaminant 10mg/L, adds above-mentioned BBM aseptic culture medium 900L after sterilization cleaning; Open reactor control unit, set temperature controls at 25 DEG C, pH 7.4, air flow 25L/min; The healthy algae liquid of aseptic transferase 45 bucket 20L enters optical-biological reaction, and keep culture condition constant, at outside scenery 5-7 days, micro-concentration of algae reaches more than 0.5g/L.
Utilize same procedure and reagent to clean and sterilization 8000L bioreactor, then add 7000L aseptic culture medium.Control condition is set: temperature controls at 25 DEG C, and pH controls 7.4, and air flow is at 100L/min, keep culture condition 5-7 days, micro-concentration of algae reaches more than 0.5g/L, keeps culture condition constant, discharge 3000L algae liquid every day, and then inject the aseptic culture medium of 3000L.
The raceway pond of step 3, Haematocoocus Pluvialls is cultivated
According to pilot scale needs, we design and have built 100m
2racetrack cultivation pool, first cultivation pool cleaned according to method mentioned above and sterilize, adding 40 tons, tap water, starting runner 10rpm, add the sodium hypochlorite solution 10L of 10% available chlorine, after 4-5 hour, bleed off thimerosal, use tap water cultivation pool, add 27 tons of aseptic waters, add Haematocoocus Pluvialls to redden the medium component cultivated, start runner 10rpm, pH is set and controls 8.0; Pour the algae liquid of 3000L from bioreactor into, start the cultivation that reddens of Haematocoocus Pluvialls, through the cultivation of 7-10 days, algae liquid gathered in the crops whole pond algae liquid after changing into scarlet, and after algae liquid results, cleaning algae pond also starts irrigation and disinfection work.
Step 4, downstream processing is carried out to Haematocoocus Pluvialls
In order to harvesting microalgae biomass, can by haematococcus pulvialis natural subsidence a whole night in algae pond, 3-5 ton is about with the algae precipitate liquid of sludge pump extraction lower floor after removing algae pond supernatant liquor second day early morning, micro-algae is allowed to continue sedimentation 8 hours in chemical barrel precipitated liquid being transferred to 5 tons, remove supernatant liquor in bucket, by link-suspended basket centrifuge centrifugal settling lower floor algae precipitate, obtain algae mud and be about 80-100 kilogram, utilized by algae mud spray-drier drying to obtain algae powder 9-11 kilogram.
Adopt cultural method of the present invention, carry out the test of cultivating haematococcus pluvialis to produce astaxanthin in pilot scale level, achieve good effect.Through comprehensive, thinking that the production cost of astaxanthin of novel process can be reduced in more than 90% times than utilizing merely reactor production cost, can Billy to cultivate with traditional raceway pond or the bioreactor toxigenic capacity of raceway pond and other type reduces by more than 60%.
Claims (2)
1. a method for ultrasound-enhanced microdisk electrode, is characterized in that, comprises the following steps:
A, select and clean a certain amount of algae, make micro-algae solid medium, the laboratory conservation expanding species of micro-algae is cultivated, first the liquid nutrient medium that suitable micro algae growth needs is chosen, add the sepharose of 1% wherein, obtain solid medium, solid medium is inserted in incubator and cultivates, culture temperature 20 DEG C, intensity of illumination 30uE/m
2/ s;
B, to choose through the aseptic micro-algae algae kind of purifying, be inoculated in described solid medium, sealing is cultivated; Last initial enlargement of carrying out micro-algae algae kind is successively cultivated, relaying is cultivated, and carries out quality control, filter out high-quality micro-algae algae kind after relaying is cultivated to micro-algae algae kind;
C, step b gained high-quality micro-algae algae kind is put into outdoor optical bio-reactor, open reactor control unit, set temperature controls at 25 DEG C, pH 7.4, air flow 25L/min; Carry out supersound process again after having reacted, ultrasonic power is 500 ~ 3000W/m
2, and carry out supersound process, obtain micro-algae plantation liquid;
D, micro-for step c gained algae plantation liquid is inoculated into outdoor racetrack cultivation pool, in racetrack cultivation pool, carry out extensive microdisk electrode, until when algae liquid becomes scarlet, collect whole pond algae liquid, pH in described outdoor racetrack cultivation pool is 8.0, and incubation time is 8 days;
E, meet d, downstream processing is carried out to whole pond algae liquid, is separated, frustule of gathering.
2. the method for ultrasound-enhanced microdisk electrode according to claim 1, is characterized in that: in step c, micro-for high-quality algae algae kind is put into outdoor gas lift duct type bioreactor and cultivates, control temperature is 12-28 DEG C, pH 7.0-8.0, air flow, at 100L/min, cultivates 5-7 days.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105132404A (en) * | 2015-10-12 | 2015-12-09 | 山东大学 | Method for fast accumulating grease through ultrasonic stimulation micro algae |
| CN106086002A (en) * | 2016-08-12 | 2016-11-09 | 扬州大学 | A kind of supersonic induced promotion micro algae growth and the method for astaxanthin accumulation |
| CN109182133A (en) * | 2018-10-25 | 2019-01-11 | 攀枝花学院 | Microalgae pure medium and the method for isolating and purifying microalgae |
| CN109401920A (en) * | 2018-12-12 | 2019-03-01 | 河西学院 | A kind of controllable slope declines the method for algae cultivating system and its both culturing microalgae |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1699548A (en) * | 2005-06-28 | 2005-11-23 | 福建师范大学 | Method for improving algae biomass and yield of biologicalactivity product thereof |
| CN104263649A (en) * | 2014-09-15 | 2015-01-07 | 李健 | Large-scale microalgae cultivation method |
-
2015
- 2015-05-14 CN CN201510244142.7A patent/CN104894102A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1699548A (en) * | 2005-06-28 | 2005-11-23 | 福建师范大学 | Method for improving algae biomass and yield of biologicalactivity product thereof |
| CN104263649A (en) * | 2014-09-15 | 2015-01-07 | 李健 | Large-scale microalgae cultivation method |
Non-Patent Citations (1)
| Title |
|---|
| 席玮芳 等: "产油微藻的筛选及大规模培养的研究进展", 《氨基酸和生物资源》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105132404A (en) * | 2015-10-12 | 2015-12-09 | 山东大学 | Method for fast accumulating grease through ultrasonic stimulation micro algae |
| CN105132404B (en) * | 2015-10-12 | 2021-03-02 | 山东大学 | Method for rapidly accumulating grease by utilizing ultrasonic stimulation microalgae |
| CN106086002A (en) * | 2016-08-12 | 2016-11-09 | 扬州大学 | A kind of supersonic induced promotion micro algae growth and the method for astaxanthin accumulation |
| CN109182133A (en) * | 2018-10-25 | 2019-01-11 | 攀枝花学院 | Microalgae pure medium and the method for isolating and purifying microalgae |
| WO2020083071A1 (en) * | 2018-10-25 | 2020-04-30 | 攀枝花学院 | Microalgae purification culture medium and method for separation and purification of microalgae |
| CN109401920A (en) * | 2018-12-12 | 2019-03-01 | 河西学院 | A kind of controllable slope declines the method for algae cultivating system and its both culturing microalgae |
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Application publication date: 20150909 |