CN104892119B - A kind of liquid microbe microbial inoculum kept soil from packing together and preparation method thereof - Google Patents
A kind of liquid microbe microbial inoculum kept soil from packing together and preparation method thereof Download PDFInfo
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- CN104892119B CN104892119B CN201510253832.9A CN201510253832A CN104892119B CN 104892119 B CN104892119 B CN 104892119B CN 201510253832 A CN201510253832 A CN 201510253832A CN 104892119 B CN104892119 B CN 104892119B
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Abstract
The present invention provides a kind of liquid microbe microbial inoculum kept soil from packing together and preparation method thereof, and the liquid microbe microbial inoculum is made up of non-microorganism raw material and microorganism raw material two parts；The non-microorganism raw material includes fulvic acid, brown sugar, urea, aspartic acid, polystyrene acid amides, EDTA iron, alginic acid, chinaberry extract solution, rapeseed extract solution, and the microorganism raw material includes bacillus subtilis, EM bacterium, lactic acid bacteria, Trichoderma and bacillusmusilaginosiengineering.After fluid present invention microbial bacterial agent 1 year, the soil weight is 1.0 1.2, and total porosity is 50 55%, and aeration porosity is 15 22%, and heavy metal in soil total amount is reduced to 220 260mg/kg from 500 600 mg/kg；Line green pepper is planted using fluid present invention microbial bacterial agent 3 years, and soil hardening does not occur.
Description
Technical field
The present invention relates to a kind of fertilizer and preparation method thereof.Specifically, it is micro- to be related to a kind of liquid kept soil from packing together
Bacteria agent and preparation method thereof, belong to agricultural technology field.
Background technology
Microbial bacterial agent is expanded for arable farming and growing and transplanting seedling area, and continuous cropping continuous cropping increases, diseases and pests of agronomic crop
The situation being on the rise, the disease-resistant bacterial manure of new bio developed using High biotechnology.
The liquid microbe microbial inoculum kept soil from packing together can improve edaphon flora, suppress soil surface characters breeding,
The root-rot of crop can effectively be prevented, found the disease such as withered.Activating soil is organic to follow with inorganic nutrient, raising fertilizer efficiency rate, promotion crop
Ring, Prolonged absorption utilize.Extraneous root strong sprout completely filled fruit.Improve soil granular structure, eliminate hardened, raising retain water and nutrients ability,
It is drought resisting, degeneration-resistant, cold-resistant, resistant to lodging.It is uninterrupted to use, soil microenvironment can be improved.Eliminate soil hardening, neutralize soda acid
Degree, reduce heavy metal-polluted soil and saline and alkaline murder by poisoning.Beneficial microbe in metabolic process is bred, can secretory cell mitogen, Yin stamp
The auximones such as acetic acid, vitamin, this acetic acid, gibberellin and amino acid isoreactivity material, strength promote extraneous root, taken root, strengthen
Root, capillary radical amount double the above.The features such as water suction, fertilizer sucting ability are strengthened, and stem is thick, robust seedling, fast transplanting slow seedling.
Patent CN201410338222.4 discloses a kind of organic fertilizer kept soil from packing together, by the original of following parts by weight
Material is made：Furfural dregs 40-50, urea 33-50, oil foot 12-16, soy sauce residues 8-12, actinolite powder 8-10, attapulgite 6-8, sulphur
Sour potassium 6-7, potassium oxide 5-6, ammonium sulfate 5-6, ammonium hydrogen carbonate 4-6, barium chloride 3-5, strontium chloride 3-5, calcium chloride 3-5, magnesium sulfate
4-5, EM bacteria agent 4-5, appropriate amount of water；The residual of heavy metal in crop is reduced, makes crop safety nontoxic, is available for consumer to trust food
With, the attapulgite nitrogen fixation of addition is good, can improved soil it is hardened, be advantageous to plant growth.
Patent CN201110363858.0 discloses a kind of lysozyme soil sterilization and exempts from the micro- life of the foster root mulch of anti-compaction of deep ploughing
Thing fertilizer, raw material components are by weight percentage：Polyacrylamide 25~60%, calcium-base bentonite 20~45% are biochemical yellow rotten
Acid 8~22%, amino acid/11~3%, chelated microelement 2~5%, wherein, calcic 13~15% in calcium-base bentonite；Chelating
In trace element, content of magnesium is 0.5~0.8%, and Zn content is 0.2~0.8%, and copper content is 0.3~0.8%, and iron content is
0.1~0.2%, manganese content is 0.1~0.2%；In amino acid, bacillus megaterium content be 0.5~1.5 hundred million/gram, gluey bud
Spore bacillus content be 0.5~100,000,000/gram；In biochemical fulvic acid, suborder bacillus content >=200,000,000/g, unwrapping wire bacterial content >=200,000,000/g, solution
Phosphorus coral Sai Shi bacillus content >=200,000,000/g, colloid bacillus content >=200,000,000/g；It is easy to use, it is not tight to environmental requirement
Lattice, can guarantee that volume increase, reduce fertilizer application amount again, reduce cost, while can also improve soil and crop quality, reduce dirty
Dye.
Patent CN201410382659.8 discloses a kind of rice bio-feritlizer, comprising as follows by weight percentage
Composition is made：Organic matter 35%-50%, dried poultrymanure 35%-50%, calcium 3%-5%, magnesium 1%-2%, phosphorus 1%-2%, complex micro organism fungicide
5%-10%；The organic matter includes powdered rice hulls or powder of straw；The complex micro organism fungicide includes bacillus subtilis, yeast
Bacterium, photosynthetic bacteria and actinomyces, the bacillus subtilis living bacteria count is 0.5 hundred million/gram -1 hundred million/gram, the saccharomycete it is effective
Viable count is 30,000/grams -5 ten thousand/gram, the photosynthetic bacteria living bacteria count be 0.5 hundred million/gram -1 hundred million/gram and the actinomyces it is effectively living
Bacterium number is 10,000/grams -2 ten thousand/gram.The nutrition of each side needed for paddy growth can be met, and chronic administration will not also cause soil plate
Knot and salinization of soil problem.
Although above patent discloses several microbial manures kept soil from packing together, but have significant limitation, deposits
The defects of and deficiency it is as follows：1st, the DeGrain of anti-compaction, although also there is anti-compaction, it is main be all fixed nitrogen,
Increase the effect of fertility, extra-nutrition element；2nd, the heavy-metal residual that can not be degraded in soil；3rd, not to hardened soil
The effect that earth improves.
The content of the invention
For problem above, the present invention provides a kind of liquid microbe microbial inoculum kept soil from packing together and preparation method thereof,
Realize following goal of the invention：
1st, line green pepper is continuously planted 3 years using fertilizer of the present invention, soil does not occur hardened；
2nd, after using fluid present invention microbial bacterial agent, degraded heavy metal in soil residual, total metalses are from 500-
600mg/kg is reduced to 220-260mg/kg；
3rd, after using fluid present invention microbial bacterial agent, improve hardened soil, reduce the soil weight to 1.0-1.2；
4th, after using fluid present invention microbial bacterial agent, total porosity is improved to 50-55%；
5th, after using fluid present invention microbial bacterial agent, aeration porosity is improved to 15-22%.
To solve problem above, the present invention uses following technical scheme：
A kind of liquid microbe microbial inoculum kept soil from packing together, including non-microorganism raw material and microorganism raw material.
It is the further improvement to above-mentioned technical proposal below：
Described non-microorganism raw material includes：Fulvic acid, brown sugar, urea, aspartic acid, polystyrene acid amides, EDTA-
Iron, alginic acid, chinaberry extract solution, rapeseed extract solution component.
The non-microorganism material component weight part ratio is：Fulvic acid 25-30 parts, brown sugar 3-5 parts, urea 10-15 parts, day
Winter propylhomoserin 5-8 part, polystyrene acid amides 0.2-0.5 parts, EDTA- iron 0.2-0.3 parts, alginic acid 1-3 parts, chinaberry extract solution 2-
5 parts, rapeseed extract solution 3-4 parts.
The microorganism raw material includes bacillus subtilis, EM bacterium, lactic acid bacteria, Trichoderma and bacillusmusilaginosiengineering.
Content of the microorganism raw material in finished product microbial inoculum be:Bacillus subtilis 4.5-5.0 hundred million/ml, EM bacterium 1.5-
2.0 hundred million/ml, hundred million/ml of lactic acid bacteria 1.0-1.5, hundred million/ml of Trichoderma 1.0-1.5, hundred million/ml of bacillusmusilaginosiengineering 0.5-1.0.
A kind of preparation method for the liquid microbe microbial inoculum kept soil from packing together, including prepare chinaberry extract solution, prepare
Rapeseed extract solution, mixing, spray bacterium step.
Described preparation chinaberry extract solution step：Chinaberry is steamed 0.5 hour, crushed, addition sterilized water, 40-45 DEG C
Extraction.
Described preparation rapeseed extract solution step：Rapeseed is steamed 1 hour, crushed, adds sterilized water, 30-37 DEG C of leaching
Carry.
Line green pepper is planted as fertilizer using described liquid microbe microbial inoculum, continuous plantation 3 years, plate does not occur for soil
Knot.
Line green pepper is planted as fertilizer using described liquid microbe microbial inoculum, after planting 1 year, heavy metal in soil total amount
220-260mg/kg is reduced to from 500-600mg/kg.
The present invention uses above technical scheme, compared with prior art, has the advantages that：
1st, using fluid present invention microbial bacterial agent, soil hardening is effectively prevent, continuous to plant line green pepper 3 years, soil does not have
There is generation hardened.
2nd, after using fluid present invention microbial bacterial agent 1 year, heavy metal in soil residual is effectively degraded, heavy metal is total
Amount is reduced to 220-260mg/kg from 500-600mg/kg.
3rd, after using fluid present invention microbial bacterial agent 1 year, it is effectively improved ecological environment of soil, the soil weight is from 1.5-
1.6 are reduced to 1.0-1.2, and total porosity rises to 50-55% from 20-30%, and aeration porosity rises to 15-22% from 7-9%.
4th, the yield and quality of line green pepper is improved using fluid present invention microbial bacterial agent, hence it is evident that reduce epidemic disease
Rate, survival rate is high, and epidemic disease incidence is 3-6%, survival rate 99.1-99.5%.Planted using fluid present invention microbial bacterial agent
Line green pepper, hence it is evident that improve line green pepper yield, line green pepper volume increase 36.5-37.7%.
5th, the raw material resources of fluid present invention microbial bacterial agent enriches, ecological, environmental protective.
6th, the cost of raw material of fluid present invention microbial bacterial agent is cheap, and technique is simple, is easy to grasp, and is adapted to large-scale production
And promote.
Embodiment
A kind of liquid microbe microbial inoculum kept soil from packing together of embodiment 1
It is made up of following two parts raw material：
（1）Non-microorganism raw material, weight part ratio are as follows：
（2）Microorganism raw material：
A kind of liquid microbe microbial inoculum kept soil from packing together of embodiment 2
It is made up of following two parts raw material：
（1）Non-microorganism raw material, weight part ratio are as follows：
（2）Microorganism raw material：
A kind of liquid microbe microbial inoculum kept soil from packing together of embodiment 3
It is made up of following two parts raw material：
（1）Non-microorganism raw material, weight part ratio are as follows：
（2）Microorganism raw material：
A kind of preparation method for the liquid microbe microbial inoculum kept soil from packing together of embodiment 4：
1st, chinaberry extract solution is prepared
Chinaberry is steamed 0.5 hour, crushed, adds the sterilized water of 50 times of weight, 40-45 DEG C extracts 42 hours, filtering, obtains
Chinaberry extract solution.
2nd, rapeseed extract solution is prepared
Rapeseed is steamed 1 hour, crushed, adds the sterilized water of 50 times of weight, 30-37 DEG C extracts 48 hours, filtering, obtains oily
Vegetable seed extract solution.
3rd, mix
Fulvic acid, brown sugar, urea, aspartic acid, polystyrene acid amides are mixed, are dissolved in weight ratio for 1:6 sterilized water
In, stir, stand 20-30 minutes, after stable, sequentially add while stirring EDTA- iron, alginic acid, chinaberry extract solution,
Rapeseed extract solution, after dissolving completely, carry out sterilization treatment.
4th, bacterium is sprayed
1st, solution be cooled to temperature for 20-40 DEG C when, successively spray into bacillus subtilis, EM bacterium, lactic acid bacteria, Trichoderma,
Bacillusmusilaginosiengineering, the bacterial content in finished product is set to be hundred million/ml of bacillus subtilis 4.5-5.0 hundred million/ml, EM bacterium 1.5-2.0, breast
Hundred million/ml of sour bacterium 1.0-1.5, hundred million/ml of Trichoderma 1.0-1.5, hundred million/ml of bacillusmusilaginosiengineering 0.5-1.0.
The line green pepper of embodiment 5 is planted
1st, soil prepares
The field of soil hardening is selected, establishes greenhouse.The soil weight of selected field is 1.5-1.6, total porosity 20-
30%, aeration porosity 7-9%, heavy metal total content are 500-600mg/kg.
2nd, nursery
Seed is carried out and hoted water treatment of seeds, 55~65 DEG C of water temperature, is soaked seed 30 minutes, is stirred in leaching.
Chemicals treatment：Soaked seed 30 minutes with 1% tertiary sodium phosphate, soaked seed 20~30 minutes with 100 times of liquid of formalin, it is standby
With.
The seed handled well is put into the hole tray full of matrix, nursery, each cavities puts a seed.
The matrix is attapulgite, oyster shell whiting, vermiculite power, peanut shell powder, pine bark powder, plant ash, blue rubble, plate indigo plant
Root powder, by 2：3：2：1：1.5：4：1：Sterilize and be made after 0.02 mass ratio mixing, moisture 50-60%.
Prepare transplanting, field planting when hole plate seedling growth is high to 30-50cm.
3rd, it is colonized
During height of seedling 30-50cm, it is colonized in greenhouse, is typically colonized in January.During field planting seedling cave has been dug in growing area, one
One plant of cave, every plant of spacing 20-30cm, line-spacing 50-60cm.
A small water is poured after field planting in time, can not broad irrigation, prevent from washing upside down the just line green pepper that has been colonized.
4th, field management line green pepper root system is undeveloped, take root it is shallow, therefore water when do not answer overlong time, forbid flood irrigation, it is necessary to
Take furrow irrigation or drip irrigation.
Irrigation quantity：The time of infertility pours water 5~6 times, each 50~60m of irrigation quantity3/ mu, keep soil moisture.Should be in line with small
The principle of water frequent irrigation, forbid broad irrigation and field ponding, noon is hot and the rainy day does not water, and this is also preventing and treating line green pepper epidemic disease
Major measure.
5th, a kind of embodiment 1-3 liquid microbe microbial inoculum kept soil from packing together is added to the water by fertilising, is mixed with water
Uniformly, then applied or drip irrigation with water punching；Mu dosage 10L or so.
6th, disease control
After the field planting of green pepper seedling is into greenhouse, be easy to get capsicum epidemic disease.Line green pepper is planted using fertilizer of the present invention, epidemic disease incidence is non-
It is often low, it is also possible to occurring, when epidemic disease occurs, 50% first frost copper 600 times of liquid of wettable powder, or 30% first frost hymexazol can be used
600 times of liquid every plant of 250 grams of amount of liquid medicine, fill 1 time ~ 2 times, 5 days ~ 7 days interval to diseased plant and surrounding plant pouring root.
Through experiment, soil can be obviously improved using fluid present invention microbial bacterial agent, and plantation line green pepper effect is good, using reality
Specific effectiveness indicator is shown in Table 1 after applying the fertilizer of a 1-3：
Table 1
Through experiment, 2 every effectiveness indicator of embodiment is best, is preferred embodiment.
Fluid present invention microbial bacterial agent effectively improves hardened soil, after planting 1 year, soil weight 1.0-1.2,
Total porosity is 50-55%, aeration porosity 15-22%.
Fluid present invention microbial bacterial agent effectively degrades heavy metal in soil residual, after planting 1 year, a huge sum of money in soil
Category total amount is 220-260mg/kg.
Line green pepper is planted using fluid present invention microbial bacterial agent, epidemic disease incidence is only 3-6%, survival rate 99.1-
99.5%, compared with using the common microbial bacterial agent kept soil from packing together, line green pepper volume increase 36.5-37.7%.
Line green pepper is continuously planted 3 years using fluid present invention microbial bacterial agent, hardened phenomenon does not occur for soil.
Finally it should be noted that：The preferred embodiments of the present invention are the foregoing is only, are not intended to limit the invention,
Although the present invention is described in detail with reference to the foregoing embodiments, for those skilled in the art, it still may be used
To be modified to the technical scheme described in previous embodiment, within the spirit and principles of the invention, that is made is any
Modification, equivalent substitution, improvement etc., should be included in the scope of the protection.
Claims (2)
- A kind of 1. liquid microbe microbial inoculum kept soil from packing together, it is characterised in that：It is former including non-microorganism raw material and microorganism Material；The non-microorganism material component weight part ratio is：It is 27 parts of fulvic acid, 4 parts of brown sugar, 12 parts of urea, 7 parts of aspartic acid, poly- Third 0.3 part of ethernamine, 0.2 part of EDTA- iron, 2 parts of alginic acid, 3 parts of chinaberry extract solution, 3 parts of rapeseed extract solution；Content of the microorganism raw material in finished product microbial inoculum be:1.8 hundred million/ml of bacillus subtilis 4.8 hundred million/ml, EM bacterium, lactic acid 1.2 hundred million/ml of bacterium, 1.2 hundred million/ml of Trichoderma, 0.8 hundred million/ml of bacillusmusilaginosiengineering；Using described liquid microbe microbial inoculum as fertilizer plant line green pepper, plantation 1 year after, heavy metal in soil total amount from 500-600mg/kg is reduced to 220mg/kg；A kind of preparation method of described liquid microbe microbial inoculum kept soil from packing together includes preparing chinaberry extract solution, prepared Rapeseed extract solution, mixing, spray bacterium step；Described preparation chinaberry extract solution step：Chinaberry is steamed 0.5 hour, crushed, adds sterilized water, 40-45 DEG C of extraction；Described preparation rapeseed extract solution step：Rapeseed is steamed 1 hour, crushed, adds sterilized water, 30-37 DEG C of extraction；Described blend step：Fulvic acid, brown sugar, urea, aspartic acid, polystyrene acid amides are mixed, being dissolved in weight ratio is 1:In 6 sterilized water, stir, stand 20-30 minutes, after stable, sequentially add EDTA- iron, alginic acid, hardship while stirring Chinaberry extract solution, rapeseed extract solution, after dissolving completely, carry out sterilization treatment.
- A kind of 2. liquid microbe microbial inoculum kept soil from packing together according to claim 1, it is characterised in that：Using described Liquid microbe microbial inoculum plant line green pepper as fertilizer, continuous plantation 3 years, soil does not occur hardened.
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| CN105665434A (en) * | 2016-01-20 | 2016-06-15 | 潍坊市新友吉土壤消毒服务专业合作社 | Soil disinfection and soil remediation combined technology |
| CN106180175B (en) * | 2016-07-11 | 2019-04-16 | 绿艺园林建设有限公司 | A method of plantation rape restoration of soil polluted by heavy metal |
| CN107089867A (en) * | 2017-06-01 | 2017-08-25 | 陕西省蒲城美尔果农化有限责任公司 | A kind of somatotrophic wheat dedicated liquid microbial manure of anti-compaction |
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| CN1107826A (en) * | 1994-11-18 | 1995-09-06 | 钟福才 | Efficient fertilizer for spraying plant root and its processing method |
| CN101792669A (en) * | 2009-11-27 | 2010-08-04 | 山东多利德生物科技有限公司 | Multi-functional biological agent containing bacillus subtilis |
| CN101993829A (en) * | 2009-08-25 | 2011-03-30 | 吉林师范大学 | Composite microbial agent |
| CN102531776A (en) * | 2011-12-23 | 2012-07-04 | 于永波 | Compound microbial fertilizer and method for producing same |
| CN103145507A (en) * | 2013-04-01 | 2013-06-12 | 张元宝 | Deep-ploughing-free compound microbial fertilizer |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1107826A (en) * | 1994-11-18 | 1995-09-06 | 钟福才 | Efficient fertilizer for spraying plant root and its processing method |
| CN101993829A (en) * | 2009-08-25 | 2011-03-30 | 吉林师范大学 | Composite microbial agent |
| CN101792669A (en) * | 2009-11-27 | 2010-08-04 | 山东多利德生物科技有限公司 | Multi-functional biological agent containing bacillus subtilis |
| CN102531776A (en) * | 2011-12-23 | 2012-07-04 | 于永波 | Compound microbial fertilizer and method for producing same |
| CN103145507A (en) * | 2013-04-01 | 2013-06-12 | 张元宝 | Deep-ploughing-free compound microbial fertilizer |
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Denomination of invention: A liquid microbial agent for preventing soil hardening and its preparation method Effective date of registration: 20221201 Granted publication date: 20180123 Pledgee: Anqiu Sub branch of China Construction Bank Corp. Pledgor: WEIFANG LVWEITE BIOLOGICAL ENGINEERING CO.,LTD. Registration number: Y2022980024397 |