CN104887728B - A kind of method for preparing the medicinal material root of Dahurain angelica - Google Patents
A kind of method for preparing the medicinal material root of Dahurain angelica Download PDFInfo
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- CN104887728B CN104887728B CN201410074633.7A CN201410074633A CN104887728B CN 104887728 B CN104887728 B CN 104887728B CN 201410074633 A CN201410074633 A CN 201410074633A CN 104887728 B CN104887728 B CN 104887728B
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Abstract
The invention discloses a kind of methods for preparing the medicinal material root of Dahurain angelica.Method provided by the invention includes the following steps：（1）The root of the plant root of Dahurain angelica is taken, removes soil；（2）Complete step（1）Afterwards, the root of the plant root of Dahurain angelica is impregnated 11 32 days in 3 10g/100mLNaCl aqueous solutions；（3）Complete step（2）Afterwards, the root of the plant root of Dahurain angelica is taken, is spontaneously dried after washing, obtains the medicinal material root of Dahurain angelica.The medicinal material root of Dahurain angelica prepared using method provided by the invention, drug rate are 23 28%, and Medicinal Materials Characters, indentification by TLC, alcohol extract and Imperatorin content reach《Pharmacopoeia of People's Republic of China》Quality standard under root of Dahurain angelica item, and content of nitrite meets relevant criterion.Method provided by the invention solves the problems, such as that the root of the plant root of Dahurain angelica in natural seasoning largely rots or goes mouldy, and can substitute sulphur fumigating system and oven drying method, have wide application value.
Description
Technical field
The present invention relates to a kind of methods for preparing the medicinal material root of Dahurain angelica.
Background technology
The medicinal material root of Dahurain angelica is the dry root of the plant root of Dahurain angelica, is a kind of common root drug material.It is excavated when summer, autumn leaf Huang
The root of the plant root of Dahurain angelica removes fibrous root and silt, dries and obtains.Drying means common at present has sulfur fumigation method, suning method, baking
Dry method.
Since the root of the root of Dahurain angelica is coarseer, it is longer to dry the time of needs naturally, meets rainy days it occur frequently that rotting, causes medicine
Material largely loses, and certain economic loss is brought to medicinal herb grower.Especially in the vast southern area in the larger China of relative humidity,
By naturally dry or dry that prepare angelica root be very unrealistic merely.
Oven drying method is although more convenient, but drying can make the color of medicine materical crude slice deeper after the root cutting of the fresh plant root of Dahurain angelica, table
Face shrinkage, and without mealiness, and before drying the water content of the medicinal material root of Dahurain angelica and the temperature of drying also have to prepared slice quality it is certain
It influences.
Sulfur fumigation method is a kind of traditional habitat processing method, solves the problems, such as medicinal material color and damages by worms, but the party
Method can decline the active constituent content of the root of Dahurain angelica, drug action reduces, and seriously affect the quality of medicinal material of the root of Dahurain angelica.
Except above-mentioned common method, there are some technological means to be used to prepare the medicinal material root of Dahurain angelica, as dried after sweating, lime buries
Hide etc..But being dried after sweating makes the volatile ingredient of the medicinal material root of Dahurain angelica that larger variation have occurred, and lime buries, and makes medicinal material
Chemical composition in the root of Dahurain angelica is changed.
In conclusion the existing drying means for being used to prepare the medicinal material root of Dahurain angelica cannot meet the need of modernization Chinese Medicine Industry
It will.Therefore, a kind of new dry technology is invented, the root of large quantities of fresh plant roots of Dahurain angelica of harvest is enable at source to obtain soon
Fast, effective drying prevents rotting and going mouldy for a large amount of fresh medicinal material raw materials, and can guarantee the quality of medicinal material after processing, is one
Item problem to be solved.
Salt marsh method is pickled mainly using vegetables as raw material using brine mostly.GB5009.33-2010《Food security
National standard nitrite in food bathes the measure of nitrate》、GB2714-2003《Catsup and pickled vegetables sanitary standard》It is required that Pickle
Nitrite（With NaNO2Meter）Content standard for≤20mg/kg, Ministry of Agriculture NY/T437-2000《Pollution-free food catsup and pickled vegetables》
The limit standard of nitrite be≤4mg/kg.
The content of the invention
The object of the present invention is to provide a kind of methods for preparing the medicinal material root of Dahurain angelica.
The method provided by the invention for preparing the medicinal material root of Dahurain angelica, includes the following steps：
（1）The root of the plant root of Dahurain angelica is taken, removes soil；
（2）Complete step（1）Afterwards, the root of the plant root of Dahurain angelica is impregnated 11-32 days in 3-10g/100mL NaCl aqueous solutions；
（3）Complete step（2）Afterwards, the root of the plant root of Dahurain angelica is taken, is spontaneously dried after washing, obtains the medicinal material root of Dahurain angelica.
The step（2）Specifically it may include following steps：
1. complete step（1）Afterwards, the root of the plant root of Dahurain angelica is soaked in the container equipped with 3-10g/100mL NaCl aqueous solutions
Bubble 1-2 days
2. after completing step 1., the NaCl aqueous solutions in container described in reject add in 3-10g/100mL NaCl water again
Solution impregnates the root of the plant root of Dahurain angelica 10-30 days.
" 3-10g/100mL NaCl aqueous solutions " described in any of the above concretely 3-4g/100mL NaCl aqueous solutions, 3-
5g/100mL NaCl aqueous solutions, 3-6g/100mL NaCl aqueous solutions, 3-8g/100mL NaCl aqueous solutions, 3-10g/100mL
NaCl aqueous solutions, 4-5g/100mL NaCl aqueous solutions, 4-6g/100mL NaCl aqueous solutions, 4-8g/100mL NaCl aqueous solutions,
4-10g/100mL NaCl aqueous solutions, 5-6g/100mL NaCl aqueous solutions, 5-8g/100mL NaCl aqueous solutions, 5-10g/
100mL NaCl aqueous solutions, 6-8g/100mL NaCl aqueous solutions, 6-10g/100mL NaCl aqueous solutions or 8-10g/100mL
NaCl aqueous solutions.
The step 2. in, soaking time concretely 10-15 days, 10-20 days, 10-30 days, 15-20 days, 15-30 days
Or 20-30 days.
NaCl aqueous solutions described in any of the above are specifically to be prepared with salt and water.
The step（2）In, the immersion carries out under air-proof condition.For example, the method for sealing can be to
Container cover lid and with water or edible oil seal.For example, the method for sealing is alternatively in vessel port plastic covering film.
The step（2）In, complete step（1）Afterwards, the root of no disease and pests harm, the plant root of Dahurain angelica having no mechanical damage is selected to carry out
It is described.
The plant root of Dahurain angelica concretely plants the plant root of Dahurain angelica in Anguo City to plant in the plant root of Dahurain angelica in Hebei province.
The plant root of Dahurain angelica concretely plants the plant root of Dahurain angelica in Suining City to plant in the plant root of Dahurain angelica in Sichuan Province.
Using the root of the salt marsh method processing plant root of Dahurain angelica in the present invention, the moisture in plant cell is made to exist using high concentration salt
The lower exudation of hyperosmosis effect of salt, so as to achieve the purpose that quickly to reduce moisture in plant, while can inhibit various micro- lifes
The activity of object prevents rotten or iso-metamorphism phenomenon of going mouldy.The medicinal material root of Dahurain angelica prepared using method provided by the invention, drug
Rate is 23-28%, and character, indentification by TLC, the content of alcohol extract and Imperatorin reach《People's Republic of China's medicine
Allusion quotation》Quality standard under root of Dahurain angelica item, and content of nitrite meets relevant criterion.Method provided by the invention solves nature and does
The problem of root of the plant root of Dahurain angelica largely rots or goes mouldy in dry method, can substitute sulphur fumigating system and oven drying method, have wide
Application value.
Specific embodiment
Following embodiment facilitates a better understanding of the present invention, but does not limit the present invention.Experiment in following embodiments
Method is conventional method unless otherwise specified.Test material used, is city unless otherwise specified in following embodiments
What field can be commercially available.Dry standard：Be spaced 6 it is small when weigh, weight does not change.Imperatorin reference substance：Chengdu
Pu Si bio tech ltd, lot number 101228-01.Isomperatorin reference substance：Upper Hiroad standing grain Pharmaceutical Technology Co., Ltd,
Lot number 120421.Pickle jar, Pickles jar, pressure stone, are scalded before use with boiling water.Pickles pond cleans one with hot boiling water before use
Time, accomplish no dirt, no-sundries.Root of Dahurain angelica control medicinal material is purchased from Chinese pharmaceutical biological product and examines and determine institute, is that the root of the plant root of Dahurain angelica passes through
Section spontaneously dries, the powder that grinding obtains.
Indentification by TLC：Substitute sample powder 0.5g, add diethyl ether 10mL, impregnates 1h, constantly shakes, and filters, filtrate
It volatilizes, residue adds ethyl acetate 1mL to make dissolving, as test solution.Root of Dahurain angelica control medicinal material 0.5g separately is taken, is made in the same way of control
Medicinal material solution.Imperatorin reference substance, Isomperatorin reference substance are taken again, add ethyl acetate that every 1mL respectively mixing containing 1mg is made
Solution, as reference substance solution.According to《Pharmacopoeia of People's Republic of China》（2010 editions）Annex VI B thin-layered chromatography is tested, and is drawn
Above-mentioned each 4 μ L of three kinds of solution are put respectively on same silica gel g thin-layer plate, with petroleum ether（30~60 DEG C）- ether（3:2）For exhibition
Agent is opened, is unfolded below 25 DEG C, is taken out, is dried, put ultraviolet lamp（365nm）Under inspect.《Pharmacopoeia of People's Republic of China》
（2010 editions）Requirement " in test sample chromatography, on position corresponding with control medicinal material chromatography and reference substance chromatography, is shown under root of Dahurain angelica item
The fluorescence spot of same color ".
Alcohol extract measures：According to《Pharmacopoeia of People's Republic of China》（2010 editions）Hot formulation under annex XA, with 50.0%
Ethanol as solvent measures the amount of extract.《Pharmacopoeia of People's Republic of China》（2010 editions）The measured value of extract under root of Dahurain angelica item
It is required that " 15.0% must not be less than ".
The assay of Imperatorin：According to《Pharmacopoeia of People's Republic of China》（2010 editions）High performance liquid chromatography under root of Dahurain angelica item
The assay method of method carries out the measure of Imperatorin content.Take angelica root powder（Cross No. three sieves）About 0.4g, it is accurately weighed, it puts
In 50mL measuring bottles, add methanol 45mL, be ultrasonically treated（Power 300W, frequency 50kHz）1h takes out, lets cool, and adds methanol to scale,
It shakes up, filters, take subsequent filtrate, obtain test solution.Take Imperatorin reference substance appropriate, it is accurately weighed, methanol is added to be configured to often
1mL contains the solution of 10 μ g, obtains reference substance solution.Using Shimadzu LC-20A high performance liquid chromatographs, it is bonded with octadecylsilane
Silica gel is filler, with methanol-water（55：45）For mobile phase, Detection wavelength 300nm, number of theoretical plate is based on Imperatorin peak
It calculates not less than 3000；It is accurate respectively to draw reference substance solution and each 20 μ L of test solution, liquid chromatograph is injected, is measured, meter
Calculate the content to get root of Dahurain angelica Imperatorin.《Pharmacopoeia of People's Republic of China》（2010 editions）This product is required to contain Europe under root of Dahurain angelica item
Peucedanin（C16H14O4）Amount be " must not be less than 0.080% ".
Nitrite Limited Doses measure：According to GB5009.33-2010《National food safety standard nitrite in food is bathed
The measure of nitrate》The assay method of the chromatography of ions carries out the determination limit of nitrite.Weigh root of Dahurain angelica sample powder（Cross three
Number sieve）5.0g is washed with ultra-pure water in 100mL volumetric flasks, and concussion shakes up and is settled to 100mL, ultrasonic extraction 30min（Every
5min concussions shake up 1 time, and solid phase is kept to be completely dispersed）, 10min is centrifuged with 10000r/min, takes supernatant；By supernatant successively
Pass through the silent winged generation that OnGuard II RP columns of U.S.'s match（Using preceding successively with methanol 10mL, water 15mL by standing activation
30min）, OnGuard II Ag columns（In advance with water 10mL by standing activation 30min）, OnGuard II Na columns（It uses in advance
Water 10mL is by standing activation 30min）, continuous eluent is collected after discarding the first eluents of 3mL, is test solution.Take nitrous
Sour sodium standard solution 5.00mL（Standard value be 200 μ g/mL food inspection sodium nitrite standard solution, GBW (E)
100156, Beijing Haian Hongmeng Standard Substance Technology Co., Ltd.）, it is settled in 100mL volumetric flasks, is configured to
10.0mg/L standard solution.It is worn using the U.S. and pacifies ICS-2000 ion chromatographs, the online generating means of outfit leacheate,
DS6 electric conductivity detectors, ASRS300-4 types suppressor, 50 μ L quantitative loops.Chromatographic column for Dionex IonPac AS19 (250mm ×
4mm), guard column is Dionex IonPac AG19 (50mm × 4mm), and 30 DEG C of column temperature, leacheate is KOH solution（Concentration is 6-
70mmol/L）, gradient 6mmol/L rises to 70mmol/L after keeping 30min in 0.1min, keeps 6min, then at
6mmol/L is down in 0.1min, keeps 6min, flow velocity 1.0mL/min.Detector is electric conductivity detector, and detection cell temperature is 35
℃.Sampling volume is 50 μ L.The reagent being related to is：Methanol（Chromatographically pure）, ultra-pure water（Resistivity is 18.2 Ω cm, Millipore
It is prepared by ultrapure water machine）.Thought by the research of methodology, the detection limit of this method（LOD, S/N=3）And quantitative limit（LOQ, S/N=
10）Respectively 3.3mg/kg and 10.0mg/kg.
The quality ÷ of drug rate=medicinal material root of Dahurain angelica is used to prepare quality × 100% of the root of the plant root of Dahurain angelica of the medicinal material root of Dahurain angelica.
It is supervised in the present invention using China Salt Industry Co., the salt of the first saltern of Shandong Han Ting production（NaCl content marks
It is shown as being more than or equal to 91.0%）Prepare NaCl aqueous solutions.The preparation method of NaCl aqueous solutions：By water it is boiled after, be cooled to room temperature,
The salt of the parts by weight such as not is added in water, stirring is completely dissolved it, filters to remove impurity if necessary, now with the current.NaCl
The concentration of aqueous solution refers to the concentration of NaCl in water.
Embodiment 1 prepares the medicinal material root of Dahurain angelica with the root of the plant root of Dahurain angelica
First, salt marsh method prepares the medicinal material root of Dahurain angelica（It is carried out in Hebei province Anguo City）
1st, mid or late October excavates the root of the plant root of Dahurain angelica from Hebei province Anguo City at random, removes soil.
2nd, after completing step 1, selection no disease and pests harm, the root having no mechanical damage take 1.5kg, fitly pile up in pickle jar
In, it is pushed down with pressure stone, adds in 3g/100mL NaCl aqueous solutions, dipped 3cm of the water surface, lid covers and seals to ensure the close of tank mouth
Feng Xing（Water or edible oil seal can be used）, pickle 1 day.
3rd, after completing step 2, the brine in pickle jar is poured out, adds in new 3g/100mL NaCl aqueous solutions, the water surface is dipped
Root 3cm, lid cover and seal to ensure the leakproofness of tank mouth（Water or edible oil seal can be used）, pickle 10 days.
4th, complete step 3 after, take out root, with the clear water of flowing wash 8 it is small when, then spontaneously dry, acquisition the medicinal material root of Dahurain angelica.
3 batches of reprocessings are carried out, does not occur to rot or mouldy iso-metamorphism phenomenon, average drug rate is 23.33%.
2nd, directly dry and prepare the medicinal material root of Dahurain angelica（It is carried out in Hebei province Anguo City）
1st, with the 1 of step 1.
2nd, after completing step 1, in the same size, no disease and pests harm, the root having no mechanical damage is selected, takes 1.5kg, spontaneously dried.
3 batches of reprocessings are carried out, a degree of rotten or mouldy iso-metamorphism phenomenon has occurred.
3rd, identify
1st, character：The character of 3 batches of medicinal material roots of Dahurain angelica prepared by step 1 is：In long pyramid type, long 15-20cm, diameter
1.5-2.5cm；Epidermis taupe brown, root head subcircular, the transverse projections of tool vertical wrinkle, supporting root trace and hole skin sample, some arrangements
Into four stringers；Matter is solid, section white or canescence, mealiness, cambium ring brown, subcircular；Gas fragrance, acrid flavour, slight bitter.Symbol
It closes《Pharmacopoeia of People's Republic of China》（2010 editions）Character requirement under root of Dahurain angelica item.
2nd, thin-layer chromatography：3 batches of medicinal material roots of Dahurain angelica prepared by step 1, corresponding with control medicinal material chromatography and reference substance chromatography
On position, the fluorescence spot of aobvious same color.Meet《Pharmacopoeia of People's Republic of China》（2010 editions）Thin layer color under root of Dahurain angelica item
Compose the requirement differentiated.
3rd, determination of extractives：The average alcohol extract content of 3 batches of medicinal material roots of Dahurain angelica prepared by step 1 is 15.24%, is met
《Pharmacopoeia of People's Republic of China》（2010 editions）Extract must not be less than 15.0% requirement under root of Dahurain angelica item.
4th, Imperatorin assay：The content of average Imperatorin of 3 batches of medicinal material roots of Dahurain angelica prepared by step 1 is
0.13%, meet《Pharmacopoeia of People's Republic of China》（2010 editions）What the amount of Imperatorin must not be less than 0.080% under root of Dahurain angelica item
It is required that.
5th, nitrite determination limit：The NaNO of 3 batches of medicinal material roots of Dahurain angelica prepared by step 12Content is below 3.3mg/kg's
Detection limit, meets GB2714-2003《Catsup and pickled vegetables sanitary standard》With Ministry of Agriculture NY/T437-2000《Pollution-free food catsup and pickled vegetables》
Nitrite limit standard.
Embodiment 2 prepares the medicinal material root of Dahurain angelica with the root of the plant root of Dahurain angelica
First, salt marsh method prepares the medicinal material root of Dahurain angelica（It is carried out in Suining City of Sichuan Province）
1st, early August excavates the root of the plant root of Dahurain angelica from Suining City of Sichuan Province at random, removes soil.
2nd, after completing step 1, selection no disease and pests harm, the root having no mechanical damage take 1.4kg, fitly pile up in pickle jar
In, it is pushed down with pressure stone, adds in 4g/100mL NaCl aqueous solutions, dipped 4cm of the water surface, lid covers and seals to ensure the close of tank mouth
Feng Xing（Water or edible oil seal can be used）, pickle 2 days.
3rd, after completing step 2, the brine in pickle jar is poured out, adds in new 4g/100mL NaCl aqueous solutions, the water surface is dipped
Root 4cm, lid cover and seal to ensure the leakproofness of tank mouth（Water or edible oil seal can be used）, pickle 15 days.
4th, complete step 3 after, take out root, with the clear water of flowing wash 16 it is small when, then spontaneously dry, acquisition medicinal material it is white
The root of Dahurian angelica.
3 batches of reprocessings are carried out, does not occur to rot or mouldy iso-metamorphism phenomenon, average drug rate is 26.24%.
2nd, directly dry and prepare the medicinal material root of Dahurain angelica（It is carried out in Suining City of Sichuan Province）
1st, with the 1 of step 1.
2nd, after completing step 1, in the same size, no disease and pests harm, the root having no mechanical damage is selected, takes 1.4kg, spontaneously dried.
3 batches of reprocessings are carried out, more serious rotten or mouldy iso-metamorphism phenomenon has occurred.
3rd, identify
1st, character：The character of 3 batches of medicinal material roots of Dahurain angelica prepared by step 1 is：In long pyramid type, long 15-20cm, diameter
1.5-2.5cm；Epidermis taupe brown, root head subcircular, the transverse projections of tool vertical wrinkle, supporting root trace and hole skin sample, some arrangements
Into four stringers；Matter is solid, section white or canescence, mealiness, cambium ring brown, subcircular；Gas fragrance, acrid flavour, slight bitter.Symbol
It closes《Pharmacopoeia of People's Republic of China》（2010 editions）Character requirement under root of Dahurain angelica item.
2nd, thin-layer chromatography：3 batches of medicinal material roots of Dahurain angelica prepared by step 1, corresponding with control medicinal material chromatography and reference substance chromatography
On position, the fluorescence spot of aobvious same color.Meet《Pharmacopoeia of People's Republic of China》（2010 editions）Thin layer color under root of Dahurain angelica item
Compose the requirement differentiated.
3rd, determination of extractives：The average alcohol extract content of 3 batches of medicinal material roots of Dahurain angelica prepared by step 1 is 20.32%, is met
《Pharmacopoeia of People's Republic of China》（2010 editions）Extract must not be less than 15.0% requirement under root of Dahurain angelica item.
4th, Imperatorin assay：The content of average Imperatorin of 3 batches of medicinal material roots of Dahurain angelica prepared by step 1 is
0.26%, meet《Pharmacopoeia of People's Republic of China》（2010 editions）What the amount of Imperatorin must not be less than 0.080% under root of Dahurain angelica item
It is required that.
5th, nitrite determination limit：The NaNO of 3 batches of medicinal material roots of Dahurain angelica prepared by step 12Content is below 3.3mg/kg's
Detection limit, meets GB2714-2003《Catsup and pickled vegetables sanitary standard》With Ministry of Agriculture NY/T437-2000《Pollution-free food catsup and pickled vegetables》
Nitrite limit standard.
Embodiment 3 prepares the medicinal material root of Dahurain angelica with the root of the plant root of Dahurain angelica
First, salt marsh method prepares the medicinal material root of Dahurain angelica（It is carried out in Hebei province Anguo City）
1st, mid or late October excavates the root of the plant root of Dahurain angelica from Hebei province Anguo City at random, removes soil.
2nd, after completing step 1, selection no disease and pests harm, the root having no mechanical damage take 5.5kg, are fitly deposited in Pickles jar
In, it is pushed down with pressure stone, adds in 6g/100mL NaCl aqueous solutions, dipped 8cm of the water surface, lid covers and seals to ensure the close of cylinder mouth
Feng Xing（Water or edible oil seal can be used）, pickle 1 day.
3rd, after completing step 2, the brine in Pickles jar is poured out, adds in new 6g/100mL NaCl aqueous solutions, the water surface is dipped
Root 8cm, lid cover and seal to ensure the leakproofness of cylinder mouth（Water or edible oil seal can be used）, pickle 20 days.
4th, complete step 3 after, take out root, with the clear water of flowing wash 20 it is small when, then spontaneously dry, acquisition medicinal material it is white
The root of Dahurian angelica.
3 batches of reprocessings are carried out, does not occur to rot or mouldy iso-metamorphism phenomenon, average drug rate is 24.73%.
2nd, identify
1st, character：The character of 3 batches of medicinal material roots of Dahurain angelica prepared by step 1 is：In long pyramid type, long 15-20cm, diameter
1.5-2.5cm；Epidermis taupe brown, root head subcircular, the transverse projections of tool vertical wrinkle, supporting root trace and hole skin sample, some arrangements
Into four stringers；Matter is solid, section white or canescence, mealiness, cambium ring brown, subcircular；Gas fragrance, acrid flavour, slight bitter.Symbol
It closes《Pharmacopoeia of People's Republic of China》（2010 editions）Character requirement under root of Dahurain angelica item.
2nd, thin-layer chromatography：3 batches of medicinal material roots of Dahurain angelica prepared by step 1, corresponding with control medicinal material chromatography and reference substance chromatography
On position, the fluorescence spot of aobvious same color.Meet《Pharmacopoeia of People's Republic of China》（2010 editions）Thin layer color under root of Dahurain angelica item
Compose the requirement differentiated.
3rd, determination of extractives：The average alcohol extract content of 3 batches of medicinal material roots of Dahurain angelica prepared by step 1 is 17.11%, is met
《Pharmacopoeia of People's Republic of China》（2010 editions）Extract must not be less than 15.0% requirement under root of Dahurain angelica item.
4th, Imperatorin assay：The content of average Imperatorin of 3 batches of medicinal material roots of Dahurain angelica prepared by step 1 is
0.14%, meet《Pharmacopoeia of People's Republic of China》（2010 editions）What the amount of Imperatorin must not be less than 0.080% under root of Dahurain angelica item
It is required that.
5th, nitrite determination limit：The NaNO of 3 batches of medicinal material roots of Dahurain angelica prepared by step 12Content is below 3.3mg/kg's
Detection limit, meets GB2714-2003《Catsup and pickled vegetables sanitary standard》With Ministry of Agriculture NY/T437-2000《Pollution-free food catsup and pickled vegetables》
Nitrite limit standard.
Embodiment 4 prepares the medicinal material root of Dahurain angelica with the root of the plant root of Dahurain angelica
First, salt marsh method prepares the medicinal material root of Dahurain angelica（It is carried out in Suining City of Sichuan Province）
1st, early August excavates the root of the plant root of Dahurain angelica from Suining City of Sichuan Province at random, removes soil.
2nd, after completing step 1, selection no disease and pests harm, the root having no mechanical damage take 5.5kg, are fitly deposited in Pickles jar
In, it is pushed down with pressure stone, adds in 10g/100mL NaCl aqueous solutions, dipped 10cm of the water surface, lid covers and seals to ensure cylinder mouth
Leakproofness（Water or edible oil seal can be used）, pickle 2 days.
3rd, after completing step 2, the brine in Pickles jar is poured out, adds in new 10g/100mL NaCl aqueous solutions, water surface leaching
Root 10cm is crossed, lid covers and seals to ensure the leakproofness of cylinder mouth（Water or edible oil seal can be used）, pickle 30 days.
4th, complete step 3 after, take out root, with the clear water of flowing wash 24 it is small when, then spontaneously dry, acquisition medicinal material it is white
The root of Dahurian angelica.
3 batches of reprocessings are carried out, does not occur to rot or mouldy iso-metamorphism phenomenon, average drug rate is 27.64%.
2nd, identify
1st, character：The character of 3 batches of medicinal material roots of Dahurain angelica prepared by step 1 is：In long pyramid type, long 15-20cm, diameter
1.5-2.5cm；Epidermis taupe brown, root head subcircular, the transverse projections of tool vertical wrinkle, supporting root trace and hole skin sample, some arrangements
Into four stringers；Matter is solid, section white or canescence, mealiness, cambium ring brown, subcircular；Gas fragrance, acrid flavour, slight bitter.Symbol
It closes《Pharmacopoeia of People's Republic of China》（2010 editions）Character requirement under root of Dahurain angelica item.
2nd, thin-layer chromatography：3 batches of medicinal material roots of Dahurain angelica prepared by step 1, corresponding with control medicinal material chromatography and reference substance chromatography
On position, the fluorescence spot of aobvious same color.Meet《Pharmacopoeia of People's Republic of China》（2010 editions）Thin layer color under root of Dahurain angelica item
Compose the requirement differentiated.
3rd, determination of extractives：The average alcohol extract content of 3 batches of medicinal material roots of Dahurain angelica prepared by step 1 is 25.19%, is met
《Pharmacopoeia of People's Republic of China》（2010 editions）Extract must not be less than 15.0% requirement under root of Dahurain angelica item.
4th, Imperatorin assay：The content of average Imperatorin of 3 batches of medicinal material roots of Dahurain angelica prepared by step 1 is
0.21%, meet《Pharmacopoeia of People's Republic of China》（2010 editions）What the amount of Imperatorin must not be less than 0.080% under root of Dahurain angelica item
It is required that.
5th, nitrite determination limit：The NaNO of 3 batches of medicinal material roots of Dahurain angelica prepared by step 12Content is below 3.3mg/kg's
Detection limit, meets GB2714-2003《Catsup and pickled vegetables sanitary standard》With Ministry of Agriculture NY/T437-2000《Pollution-free food catsup and pickled vegetables》
Nitrite limit standard.
Embodiment 5 prepares the medicinal material root of Dahurain angelica with the root of the plant root of Dahurain angelica
First, salt marsh method prepares the medicinal material root of Dahurain angelica（It is carried out in Hebei province Anguo City）
1st, mid or late October excavates the root of the plant root of Dahurain angelica from Hebei province Anguo City at random, removes soil.
2nd, after completing step 1, selection no disease and pests harm, the root having no mechanical damage take 50kg, are fitly deposited in pickles pond
In, it is pushed down with pressure stone, adds in 5g/100mL NaCl aqueous solutions, dipped 20cm of the water surface, pond is covered on face with plastic foil, pickles 1
My god.
3rd, after completing step 2, extract the brine in pickles pond out, add in new 5g/100mL NaCl aqueous solutions, the water surface is dipped
Root 20cm, pond are covered on face with plastic foil, are pickled 10 days.
4th, complete step 3 after, with the clear water of flowing wash 12 it is small when, then spontaneously dry, acquisition the medicinal material root of Dahurain angelica.
3 batches of reprocessings are carried out, does not occur to rot or mouldy iso-metamorphism phenomenon, average drug rate is 24.0%.
2nd, identify
1st, character：The character of 3 batches of medicinal material roots of Dahurain angelica prepared by step 1 is：In long pyramid type, long 15-20cm, diameter
1.5-2.5cm；Epidermis taupe brown, root head subcircular, the transverse projections of tool vertical wrinkle, supporting root trace and hole skin sample, some arrangements
Into four stringers；Matter is solid, section white or canescence, mealiness, cambium ring brown, subcircular；Gas fragrance, acrid flavour, slight bitter.Symbol
It closes《Pharmacopoeia of People's Republic of China》（2010 editions）Character requirement under root of Dahurain angelica item.
2nd, thin-layer chromatography：3 batches of medicinal material roots of Dahurain angelica prepared by step 1, corresponding with control medicinal material chromatography and reference substance chromatography
On position, the fluorescence spot of aobvious same color.Meet《Pharmacopoeia of People's Republic of China》（2010 editions）Thin layer color under root of Dahurain angelica item
Compose the requirement differentiated.
3rd, determination of extractives：The average alcohol extract content of 3 batches of medicinal material roots of Dahurain angelica prepared by step 1 is 18.18%, is met
《Pharmacopoeia of People's Republic of China》（2010 editions）Extract must not be less than 15.0% requirement under root of Dahurain angelica item.
4th, Imperatorin assay：The content of average Imperatorin of 3 batches of medicinal material roots of Dahurain angelica prepared by step 1 is
0.13%, meet《Pharmacopoeia of People's Republic of China》（2010 editions）What the amount of Imperatorin must not be less than 0.080% under root of Dahurain angelica item
It is required that.
5th, nitrite determination limit：The NaNO of 3 batches of medicinal material roots of Dahurain angelica prepared by step 12Content is below 3.3mg/kg's
Detection limit, meets GB2714-2003《Catsup and pickled vegetables sanitary standard》With Ministry of Agriculture NY/T437-2000《Pollution-free food catsup and pickled vegetables》
Nitrite limit standard.
Embodiment 6 prepares the medicinal material root of Dahurain angelica with the root of the plant root of Dahurain angelica
First, salt marsh method prepares the medicinal material root of Dahurain angelica（It is carried out in Suining City of Sichuan Province）
1st, early August excavates the root of the plant root of Dahurain angelica from Suining City of Sichuan Province at random, removes soil.
2nd, after completing step 1, selection no disease and pests harm, the root having no mechanical damage take 80kg, are fitly deposited in pickles pond
In, it is pushed down with pressure stone, adds in 8g/100mL NaCl aqueous solutions, dipped 20cm of the water surface, pond is covered on face with plastic foil, pickles 2
My god.
3rd, after completing step 2, extract the brine in pickles pond out, add in new 8g/100mL NaCl aqueous solutions, the water surface is dipped
Root 20cm, pond are covered on face with plastic foil, are pickled 15 days.
4th, complete step 3 after, with the clear water of flowing wash 48 it is small when, then spontaneously dry, acquisition the medicinal material root of Dahurain angelica.Carry out 3
Reprocessing is criticized, does not occur to rot or mouldy iso-metamorphism phenomenon, average drug rate is 26.3%.
2nd, identify
1st, character：The character of 3 batches of medicinal material roots of Dahurain angelica prepared by step 1 is：In long pyramid type, long 15-20cm, diameter
1.5-2.5cm；Epidermis taupe brown, root head subcircular, the transverse projections of tool vertical wrinkle, supporting root trace and hole skin sample, some arrangements
Into four stringers；Matter is solid, section white or canescence, mealiness, cambium ring brown, subcircular；Gas fragrance, acrid flavour, slight bitter.Symbol
It closes《Pharmacopoeia of People's Republic of China》（2010 editions）Character requirement under root of Dahurain angelica item.
2nd, thin-layer chromatography：3 batches of medicinal material roots of Dahurain angelica prepared by step 1, corresponding with control medicinal material chromatography and reference substance chromatography
On position, the fluorescence spot of aobvious same color.Meet《Pharmacopoeia of People's Republic of China》（2010 editions）Thin layer color under root of Dahurain angelica item
Compose the requirement differentiated.
3rd, determination of extractives：The average alcohol extract content of 3 batches of medicinal material roots of Dahurain angelica prepared by step 1 is 23.13%, is met
《Pharmacopoeia of People's Republic of China》（2010 editions）Extract must not be less than 15.0% requirement under root of Dahurain angelica item.
4th, Imperatorin assay：The content of average Imperatorin of 3 batches of medicinal material roots of Dahurain angelica prepared by step 1 is
0.20%, meet《Pharmacopoeia of People's Republic of China》（2010 editions）What the amount of Imperatorin must not be less than 0.080% under root of Dahurain angelica item
It is required that.
5th, nitrite determination limit：The NaNO of 3 batches of medicinal material roots of Dahurain angelica prepared by step 12Content is below 3.3mg/kg's
Detection limit, meets GB2714-2003《Catsup and pickled vegetables sanitary standard》With Ministry of Agriculture NY/T437-2000《Pollution-free food catsup and pickled vegetables》
Nitrite limit standard.
Claims (15)
1. a kind of method for preparing the medicinal material root of Dahurain angelica, includes the following steps：
（1）The root for the plant root of Dahurain angelica for take no disease and pests harm, having no mechanical damage removes soil；
（2）Complete step（1）Afterwards, the root of the plant root of Dahurain angelica is impregnated 11-32 days in 3-10 g/100 mL NaCl aqueous solutions；
（3）Complete step（2）Afterwards, the root of the plant root of Dahurain angelica is taken, is spontaneously dried after washing, obtains the medicinal material root of Dahurain angelica；
The step（2）Include the following steps：
1. complete step（1）Afterwards, the root of the plant root of Dahurain angelica is impregnated into 1- in the container equipped with 3-10g/100mL NaCl aqueous solutions
2 days；
2. after completing step 1., the NaCl aqueous solutions in container described in reject add in 3-10g/100mL NaCl aqueous solutions again,
The root of the plant root of Dahurain angelica is impregnated 10-30 days；
The immersion carries out under air-proof condition；
The container is cleaned using preceding with boiling water；
The NaCl aqueous solutions are now with the current；
The NaCl aqueous solutions are prepared as follows what is obtained：By water it is boiled after, be cooled to room temperature, add in salt, stirring
It is completely dissolved, then filters to remove impurity.
2. the method as described in claim 1, it is characterised in that：" the 3-10g/100mL NaCl aqueous solutions " is 3-4g/
100mL NaCl aqueous solutions.
3. the method as described in claim 1, it is characterised in that：" the 3-10g/100mL NaCl aqueous solutions " is 3-5g/
100mL NaCl aqueous solutions.
4. the method as described in claim 1, it is characterised in that：" the 3-10g/100mL NaCl aqueous solutions " is 3-6g/
100mL NaCl aqueous solutions.
5. the method as described in claim 1, it is characterised in that：" the 3-10g/100mL NaCl aqueous solutions " is 3-8g/
100mL NaCl aqueous solutions.
6. the method as described in claim 1, it is characterised in that：" the 3-10g/100mL NaCl aqueous solutions " is 4-5g/
100mL NaCl aqueous solutions.
7. the method as described in claim 1, it is characterised in that：" the 3-10g/100mL NaCl aqueous solutions " is 4-6g/
100mL NaCl aqueous solutions.
8. the method as described in claim 1, it is characterised in that：" the 3-10g/100mL NaCl aqueous solutions " is 4-8g/
100mL NaCl aqueous solutions.
9. the method as described in claim 1, it is characterised in that：" the 3-10g/100mL NaCl aqueous solutions " is 4-10g/
100mL NaCl aqueous solutions.
10. the method as described in claim 1, it is characterised in that：" the 3-10g/100mL NaCl aqueous solutions " is 5-6g/
100mL NaCl aqueous solutions.
11. the method as described in claim 1, it is characterised in that：" the 3-10g/100mL NaCl aqueous solutions " is 5-8g/
100mL NaCl aqueous solutions.
12. the method as described in claim 1, it is characterised in that：" the 3-10g/100mL NaCl aqueous solutions " is 5-10g/
100mL NaCl aqueous solutions.
13. the method as described in claim 1, it is characterised in that：" the 3-10g/100mL NaCl aqueous solutions " is 6-8g/
100mL NaCl aqueous solutions.
14. the method as described in claim 1, it is characterised in that：" the 3-10g/100mL NaCl aqueous solutions " is 6-10g/
100mL NaCl aqueous solutions.
15. the method as described in claim 1, it is characterised in that：" the 3-10g/100mL NaCl aqueous solutions " is 8-10g/
100mL NaCl aqueous solutions.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102210728A (en) * | 2010-05-21 | 2011-10-12 | 成都中医药大学 | Method for processing angelica dahurica in producing area |
| CN102258553A (en) * | 2010-05-24 | 2011-11-30 | 成都中医药大学 | Method for processing radix angelicae decoction pieces in producing area |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102210728A (en) * | 2010-05-21 | 2011-10-12 | 成都中医药大学 | Method for processing angelica dahurica in producing area |
| CN102258553A (en) * | 2010-05-24 | 2011-11-30 | 成都中医药大学 | Method for processing radix angelicae decoction pieces in producing area |
Non-Patent Citations (1)
| Title |
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| "几种盐制中药炮制方法的改进";叶玲玲;《浙江临床医学》;19991231;第1卷(第6期);430 * |
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