CN104873175A - System and method for diffused optical tomography and photoacoustic tomography combined measurement - Google Patents
System and method for diffused optical tomography and photoacoustic tomography combined measurement Download PDFInfo
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Abstract
The invention discloses a system and method for diffused optical tomography and photoacoustic tomography combined measurement. The system comprises a diffused optical tomography measurement part (1), an imitating CT space scanning system (2), a photoacoustic tomography measurement part (3) and an industrial control computer (4), wherein the diffused optical tomography measurement part (1) and the photoacoustic tomography measurement part (3) are jointly connected with the imitating CT space scanning system (2) to form an integrated multi-mode measurement system, and system automatic control software of the industrial control computer (4) is used for achieving automatic detection and data collection of a diffused optical signal and a photoacoustic signal. Compared with the prior art, by means of the system and method for the diffused optical tomography and photoacoustic tomography combined measurement, the defects of an existing single-mode imaging technique are overcome, the defect that for the prior art, the spatial resolution and the quantitative feature cannot be achieved simultaneously is overcome, the imaging method can achieve the spatial resolution of the photoacoustic tomography technique and the quantitative precision of the diffused optical tomography technique, and therefore the high-quality optical parameter imaging function is achieved.
Description
Technical field
The present invention relates to the application of optical image technology on biomedical direction, particularly a kind of diffuse optical chromatography and optoacoustic chromatography combined measurement system, multi-modal measuring method.
Background technology
Along with the development of biology, medical science, photoelectric technology and computer technology, various advanced medical imaging device constantly presents, for clinical diagnosis provides the medical image of various modes.Because imaging mechanism is different and the high complexity of human tissue structure, the medical image of single-mode can not provide the enough information required for doctor, and the information that the medical image of different modalities provides can not cover, mutually supplementary.Therefore, in clinical diagnosis and treatment, the development for multi-modal medical imaging technology has very important meaning.
Along with the development of various modes medical imaging modalities, modern medicine study developing direction also little by little changes, namely from by traditional anatomy formation method, established disease is diagnosed, be developed to the Forming Mechanism utilizing medical science functional imaging technology to disclose disease.Optical imagery is as a kind of functional imaging means, because there is comprehensive advantage that other mode cannot have concurrently on the key indexs such as sensitivity, specificity, contrast, quantitative and radiogical safety and receiving much attention, be used widely in field of biomedical research.Especially along with the rise of near infrared tissue spectral technique (near-infrared spectroscopy, NIRS), the optical function detection technique for biological tissue obtains and develops rapidly.Wherein, be based upon diffuse optical tomography technology (the diffuse opticaltomography on NIRS basis, DOT) be Non-invasive detection technology for organizer biochemical function information, the three-dimensional real-time quantitative that can realize biological tissue deep index of physiology and pathology relevant information obtains.But be subject to the impact organizing height scattering properties, such pure optical image technology cannot get both on imaging depth and spatial resolution, and its spatial resolution is limited in the 5%-10% of image tissue size; Photoacoustic tomography technology (photoacoustictomography, PAT) method based on optoacoustic effect combines the advantage of optical imagery and ultra sonic imaging effectively, is a kind of with ultrasonic vehicular bio-photon formation method.The method adopts pulsed laser irradiation biological tissue, produces ultrasonic (optoacoustic) signal after tissue resorption light energy, by measuring the photoacoustic signal carrying biological organism optical Absorption Characteristics information, can reconstruct the light energy absorption distributed image of organizer.Due to features such as the low decay of ultrasound wave and high-penetrabilities, considerably reduce the impact of organizer scattering effect, PAT method can carry out the 26S Proteasome Structure and Function imaging of high spatial resolution and high-contrast to deep tissues.But conventional P AT technology calculates the spatial distribution of organizer light energy absorption density by rebuilding initial acoustic pressure, and supposing that the absorptance of organizer and light energy absorption density are linear, finally obtaining absorptance reconstruction image.When towards deep tissues imaging, because complex biological organizes body absorption and Scatter inhomogeneity, this linear hypothesis relation and practical situation are not inconsistent, and in organizer, photic-energy transfer feature becomes affects optical parametric and rebuild quantitative key issue.
Therefore, find a kind of high-contrast advantage of both having possessed optical function imaging, meet again the medical imaging procedure of the quantitative and high spatial resolution requirement of imaging, be biomedical imaging field problem demanding prompt solution simultaneously.In other words, the high spatial resolution characteristic of the quantitative and PAT imaging technique of the height that the method needs to have DOT imaging technique, namely possess two kinds of imaging mode chiefs, complement each other deficiency, thus obtain the optical function image of ideal quantitative and spatial resolution.The complementary information that different modalities imaging technique provides is integrated, on piece image, not only can express the information of multiple image simultaneously, a kind of brand-new multi-modality imaging method can also be formed by the innovation on theoretical learning, the image that so the method provides can provide more and more detailed and accurate physiological and pathological information for doctor makes definite diagnosis, also can provide more intuitively the observation of focus and the diagnosis of disease for doctor, comprehensively and clearly basis for estimation, improve the recall rate of disease.
Summary of the invention
In order to overcome the problem of above-mentioned prior art, the present invention proposes a kind of diffuse optical chromatography and optoacoustic chromatography combined measurement system and method, utilize the means of the combined measurement of diffuse optical chromatography and optoacoustic chromatography, realize multi-modal measurement, finally can obtain the distributed image of optical parametric in two dimension or three dimensions of objective body.
The present invention proposes the combined measurement system of a kind of diffuse optical chromatography and optoacoustic chromatography, and this system comprises diffuse optical laminar analysis measurement part (1), imitative CT spacescan system (2), optoacoustic chromatography measure portion (3) and industrial control computer (4); Wherein: described diffuse optical laminar analysis measurement part (1) and described optoacoustic chromatography measure portion (3) are connected the complete multi-modal measuring system of imitative CT spacescan system (2) formation one jointly; System auto-control software, date processing and image reconstruction software in described industrial control computer (4) are for realizing automatization's detection of diffuse optical signal and photoacoustic signal, data acquisition and pretreatment and image reconstruction; Wherein, auto-control software for realizing the sequencing contro to diffuse optical chromatography and optoacoustic chromatography combined measurement system components, to realize the automatization of measuring process; Date processing and image reconstruction software are used for carrying out pretreatment to associating measuring system measurement data, and adopt image reconstruction algorithm to carry out image reconstruction to diffused light measurement data and optoacoustic measurement data, thus obtain objective body diffuse optical chromatography and optoacoustic chromatography bimodal combines reconstruction image;
Described diffuse optical laminar analysis measurement part (1) further comprises optical receiving system (11), stable light source system (12), filter system (13) and detection system (14); Described optoacoustic chromatography measure portion (3) further comprises optoacoustic detection system (31), light-pulse generator system (32) and data collecting system (33).
Described imitative CT spacescan system comprises water tank (21), immerse in water tank the electrical-controlled lifting platform that arranges and turntable (22), imaging chamber (23) and for carry first ultrasonic transducer (311), the second ultrasonic transducer (312) to realize the automatically controlled annular detection turntable (24) of signal circular scanning, objective body (5) is placed in columniform imaging chamber (23), and imaging chamber (23) maintain cylindrical shape and can ensure that ultrasonic signal has effect spread; Imaging chamber (23) is fixed on electrical-controlled lifting platform and turntable (22), by the control to lifting spacing and the anglec of rotation, adjust different imaging surface (6) upper Different Light incident angle, i.e. any setting of implementation space sample rate; In described water tank (21), the water of carrying is as ultrasonic coupling liquid, is dipped in coupling liquid by whole scanning system, for the propagation of acoustic signal and the decay of minimizing ultrasonic signal.
Described diffuse optical laminar analysis measurement part (1) also comprises optical receiving system (11), stable light source system (12), automatically controlled filter system (13) and detection system (14); Wherein:
Described optical receiving system (11) is uniformly distributed 8 detection optical fibers by the first-class angle intervals in same detection plane position of objective body (5) offside and forms, and adopts the semicircle structure of fiber_optic coaxial with imaging chamber to fix;
Described stable light source system (12) comprises the first stable state semiconductor laser (121), the second stable state semiconductor laser (122) that two wavelength are respectively λ 1 and λ 2, and it is fine to connect axis incident light; Incident illumination vertical incidence after collimator (123) is surperficial to objective body (5); Be linked in the different passages of 8:1 photoswitch (111) respectively, 8:1 photoswitch (111) emergent light is through collimator (112) extremely automatically controlled filter system (13);
Described automatically controlled filter system (13) comprises filter wheel controller (131) and automatically controlled filter wheel (132), and emergent light is incident to detection system (14) by automatically controlled filter system;
Described detection system (14) comprises photomultiplier tube (141) and counting module (142), described photomultiplier tube (141) receiving optical signals also converts the signal of telecommunication to, is undertaken processing and record by counting module (142).
Described optoacoustic chromatography measure portion (3) also comprises optoacoustic detection system (31), light-pulse generator system (32) and data collecting system (33); Wherein:
Described light-pulse generator system (32) adopts the distributed more incident mode of pulse laser (321) to realize the uniform irradiation of high energy pulse incident illumination to objective body (5) surface, connect one group of both arms fibre bundle (322), 1/4 semicircular domain of fibre bundle transmission laser difference uniform irradiation imaging surface;
Described optoacoustic detection system (31) is made up of first ultrasonic transducer (311) of carry on automatically controlled annular detection turntable (24) and the second ultrasonic transducer (312), and relative incident light source is positioned at reflection measurement face; The circular scanning position of its probe is adjusted by automatically controlled annular detection turntable (24); The photoacoustic signal of objective body (5) outgoing changes into the signal of telecommunication by after the first ultrasonic transducer (311) and the second ultrasonic transducer (312) detection, is sent to data collecting system (33);
Described data collecting system (33) comprises preamplifier (331) and high-speed data acquisition card (332).
The present invention also proposes the union measuring method of a kind of diffuse optical chromatography and optoacoustic chromatography, and the method comprises the following steps:
Step (1), the stable light source system of selected wavelength is connected to axis incident light fibre, incident optical after collimator vertical incidence to target surface, 8 detection optical fibers are uniformly distributed in the first-class angle intervals in position, isoplanar of its offside, be linked in the different passages of multichannel optical switch respectively, obtained the emergent light signal of different detecting location by the switching of 8:1 photoswitch; 8:1 photoswitch emergent light is by being incident to photomultiplier tube after automatically controlled filter wheel after collimating device collimation, and photomultiplier tube receiving optical signals also converts the signal of telecommunication to and undertaken processing and record by counting module.
Step (2), pulse laser produce pulse laser as photo-acoustic excitation light source, by both arms fibre bundle, exciting light is sent to the different incoming positions of imaging surface, vertical incidence is to target surface to be measured, excite the photoacoustic signal of generation through ultrasonic coupling liquid by pulse laser, be sent to ultrasonic transducer and be converted into the signal of telecommunication, the signal of telecommunication is after preamplifier, carry out data acquisition by high-speed data acquisition card, the data gathered the most at last send industrial control computer to and process; By the circular scanning position of automatically controlled annular detection turntable adjustment ultrasonic transducer, repeat above measuring process, finally can obtain the photoacoustic signal of the lower objective body outgoing to be measured of different angles projection;
Step (3), obtain the diffuse optical signal under single source incident angle by step (1), and then collect by step (2) photoacoustic signal inspired in objective body to be measured; Utilize the system auto-control software in industrial control computer to regulate the automatically controlled turntable in imitative CT spacescan system, thus obtain new light source incidence angle, repeat step (1) and (2), obtain the diffuse optical signal on a two-dimensional imaging face under any light source incidence angle and photoacoustic signal; Reutilization system auto-control software regulates electrical-controlled lifting platform and turntable, continue to repeat above step, just can obtain the information on any imaging surface of objective body to be measured, get up the information combination on all imaging surfaces just to obtain the three-dimensional information of whole objective body; Send the information of acquisition to date processing and image reconstruction software, finally can obtain the distributed image of optical parametric in two dimension or three dimensions of objective body.
Compared with prior art, the present invention overcomes the deficiency of existing single modality technology, make up prior art spatial resolution and quantitative on the defect that cannot get both, make the spatial resolution of formation method with optoacoustic chromatographic technique and the quantitative accuracy of diffuse optical chromatographic technique, then realize high-quality optical parametric imaging function.
Accompanying drawing explanation
Fig. 1 is the combined measurement system block diagram of diffuse optical chromatography and optoacoustic chromatography; Wherein Reference numeral: 1, diffuse optical laminar analysis measurement part; 2, imitative CT spacescan system; 3, optoacoustic chromatography measure portion; 4, industrial control computer; 11, optical receiving system; 12, stable light source system; 13, automatically controlled filter system; 14, detection system; 31, optoacoustic detection system; 32, light-pulse generator system; 33, data collecting system;
Fig. 2 is the combined measurement system structure schematic diagram of dual wavelength double frequency wide diffuse optical laminar analysis measurement part and optoacoustic chromatography combined measurement part composition; Wherein Reference numeral: 111,8:1 photoswitch; 112, collimator; 121, the first stable state semiconductor laser; 122, the second stable state semiconductor laser; 131, filter wheel controller and 132, automatically controlled filter wheel; 141, photomultiplier tube; 142, counting module; 311, the first ultrasonic transducer; 312, the second ultrasonic transducer; 321, pulse laser; 322, both arms fibre bundle; 331, preamplifier; 332, high-speed data acquisition card; 5, objective body;
Fig. 3 is the structural representation of imitative CT spacescan system; Wherein Reference numeral: 21, water tank; 22, electrical-controlled lifting platform and turntable; 23, imaging chamber; 24, automatically controlled annular detection turntable; 6, imaging surface.
Detailed description of the invention
Below in conjunction with accompanying drawing, the specific embodiment of the present invention is described in detail, if these embodiments exist exemplary content, should not be construed to limitation of the present invention.
Embodiment 1:
As shown in Figure 2, specific embodiment of the invention case 1 is the combined measurement system structure of dual wavelength double frequency wide diffuse optical laminar analysis measurement part and optoacoustic chromatography combined measurement part composition.
Diffuse optical laminar analysis measurement part implementation process (as depicted in figs. 1 and 2): two wavelength are respectively the first stable state semiconductor laser (121) of λ 1 and λ 2, the axis incident light that second stable state semiconductor laser (122) (mean power <10mW) connects core diameter 62.5 μm is fine, incident illumination after collimator (123) vertical incidence to objective body (5) surface (setting this incident angle as 0 °), the optical receiving system (11) that the detection optical fiber structure being uniformly distributed 8 core diameters 500 μm by the first-class angle intervals in position, isoplanar on 101.25 ° ~ 258.75 °, its opposite forms receives (adopting the semicircle structure of fiber_optic coaxial with imaging chamber to fix), be linked into the different passages interior (being obtained the optical signal of different detecting location by the switching of photoswitch) of 8:1 photoswitch (111) respectively, photoswitch emergent light is through collimator (112) extremely automatically controlled filter system, automatically controlled filter system comprises filter wheel controller (131) and realizes the automatically controlled filter wheel (132) of different optical filter handoff functionality (neutral-density filter being loaded with the differential declines intensity of OD=1 ~ 5 makes detection system be operated in the range of linearity with strong diffused light signal of decaying), emergent light is incident to detection system by automatically controlled filter system, detection system comprises photomultiplier tube (PMT) (141) and counting module (142) (photon counter), PMT receiving optical signals and convert to the signal of telecommunication by counting module (142) (adopt programmable logic technology realize counting gate-width and the adjustable design time of integration) carry out processing and record.Optoacoustic chromatography measure portion implementation process (as depicted in figs. 1 and 2): light-pulse generator system adopts distributed more incident mode to realize the uniform irradiation of high-energy incident illumination to objective body (5) surface.Light-pulse generator system (32) adopts pulse laser (321) (the OPO laser instrument of Q-switch Nd:YAG laser instrument (wavelength 532nm) pumping, tunable wave length scope 600 ~ 900nm), intend selecting two wavelength close with DOT measure portion, pulse width≤10ns, repetition rate 10/20Hz, pulse energy ~ 50mJ.Light-pulse generator system connects one group of both arms fibre bundle (322), 1/4 semicircular domain of fibre bundle transmission laser difference uniform irradiation imaging surface.Optoacoustic detection system (31) is made up of two broad-band ultrasonic transducers (311), (312) (adopting water logging non-agglomerated ultrasonic probe); Two ultrasonic transducers (311), (312) relative incident light sources are positioned at reflection measurement face, to ensure the accuracy of close to sources area light acoustical signal measurement, and in conjunction with effective covering of the overall search coverage photoacoustic signal of imitating CT scanning mode realize target body.The receiving plane diameter of each ultrasonic transducer is about 6mm, and mid frequency is respectively 2.25MHz and 10MHz, and pass band is respectively 1.35MHz and 7.28MHz.In order to obtain the Multi-angle ultrasound projection under same light source, automatically controlled annular detection turntable (24) is utilized to adjust the scanning position of ultrasonic probe, adjustment direction scope is respectively first quartile and the fourth quadrant interior (setting the stable light source incident angle of DOT part as 0 °) of imaging surface, by selecting the scanning projection number under different angles spacing adjustment same light source incident angle.The photoacoustic signal of objective body (5) outgoing changes into the signal of telecommunication by two ultrasonic transducers (311), (312) after detecting, and is resent to data collecting system (33).Data collecting system comprises multichannel signal preamplifier (331) and multi-channel high-speed data capture card (332) (arranging sample frequency is 100MHz); The photoacoustic signal that receives is sent in the different passages of signal preamplifier by ultrasonic transducer group, then gathers the signal of different passage by multi-channel high-speed data capture card (332), and to be sent to Industry Control calculating etc. pending for data the most at last.The frequency response of signal preamplifier and the sample frequency of data collecting card are selected according to the dominant frequency of ultrasonic transducer.
Fig. 3 is the structural representation of imitative CT spacescan system: objective body to be measured is placed in columniform imaging chamber (23), imaging chamber (23) intends adopting epoxide resin material and pvdf membrane jointly to make, what can either maintain that cylindrical shape can ensure again ultrasonic signal has effect spread, its diameter is about 2-3cm, wall thickness≤1mm.Imaging chamber (23) is fixed on electrical-controlled lifting platform and turntable (22), by the control to lifting spacing and the anglec of rotation, adjust the upper Different Light incident angle of different imaging surface (6), namely adopt the scan pattern of this imitative CT to carry out any setting of implementation space sample rate.In addition, in order to be conducive to the propagation of acoustic signal and reduce the decay of ultrasonic signal, whole scanning survey structure is dipped in water tank (21).Utilize electrical-controlled lifting platform and turntable to realize the multi-angle scanning on three dimensions, freely control space sampling densities by adjustment sweep spacing and scope.Whole scanning system, as ultrasonic coupling liquid, is dipped in coupling liquid by the water carried in water tank, thus is conducive to photoacoustic signal propagation and reduces the decay of ultrasonic signal.
Embodiment 2:
System described in embodiment 1 is utilized to carry out diffuse optical chromatography and the optoacoustic chromatography combined measurement of Animal tissue model.This system can realize focal area high-contrast and high spatial resolution imaging, can monitor tumor neogenetic blood vessels and blood oxygen situation, and the observing at body of endogenous or external source Specific marker.Illustrate, when adopting indocyanine green (indocyanine green, ICG) to absorb contrast medium as external source in living small animal experiment, operation wavelength λ
1absorption spectrum peak wavelength according to ICG is set to 785nm.In addition, for effectively obtaining blood oxygen information in organizer, system works wavelength X
2propose according to hemoglobin isobestic point (about 805nm) and be set to 830nm.During measurement, objective body to be measured is placed in column type imaging chamber, imaging chamber and the space between objective body adopt imitative organizer to mate solution (intending adopting Intralipid aqueous solution to add India Ink to be mixed) and fill.The implementation step of diffuse optical laminar analysis measurement part and optoacoustic chromatography measure portion in reference embodiment 1, first measuring process obtains operation wavelength by diffuse optical laminar analysis measurement part is λ
1single source incident angle under diffuse optical signal; The photoacoustic signal that inspires of objective body is gathered again by optoacoustic chromatography measure portion; Then utilize the system auto-control software in industrial control computer to regulate the automatically controlled turntable in imitative CT spacescan system, thus obtain new light source incidence angle; Repeated measure step, can obtain the diffuse optical signal on one group of two-dimensional imaging tomography and photoacoustic signal respectively; Then be λ at same imaging fault wavelength of finishing the work
2diffuse optical laminar analysis measurement and optoacoustic laminar analysis measurement, operation wavelength is λ
2measuring process and λ
1measuring process under wavelength is identical; Reutilization system auto-control software regulates electrical-controlled lifting platform, continues to repeat above step, just can obtain the information on other imaging faults of objective body, all fault information is integrated the three-dimensional information obtaining objective body.Send the information of acquisition to date processing and image reconstruction software, finally can obtain objective body respectively at λ
1and λ
2distributed image on the two dimension of the optical parametric under operation wavelength or three dimensions.The present embodiment obtains at λ respectively by adopting dual-wavelength measurement mode
1and λ
2optical absorption parameter under operation wavelength
with
distribution, carries out the calculating of blood oxygen saturation then.Blood oxygen saturation SaO
2refer to that in blood, the capacity of HbO2 Oxyhemoglobin accounts for the percentage ratio of whole combinative hemoglobin, hemoglobin has HbO2 Oxyhemoglobin HbO
2with reduced hemoglobin Hb two kinds of forms.Because the major optical absorbing material in biological tissue under this dual wavelength is hemoglobin, then optical absorption parameter and HbO
2can be expressed as with Hb concentration relationship formula
Wherein
with
be respectively HbO in blood
2molar absorption coefficient and concentration; ε
hband C
hbmolar absorption coefficient and the concentration of Hb in blood respectively.Can be obtained by formula (1)
HbO can be tried to achieve respectively
2with the concentration of Hb, thus can calculate blood oxygen saturation in organizer, concrete formula is
Claims (5)
1. the combined measurement system of a diffuse optical chromatography and optoacoustic chromatography, it is characterized in that, this system comprises diffuse optical laminar analysis measurement part (1), imitative CT spacescan system (2), optoacoustic chromatography measure portion (3) and industrial control computer (4); Wherein: described diffuse optical laminar analysis measurement part (1) and described optoacoustic chromatography measure portion (3) are connected the complete multi-modal measuring system of imitative CT spacescan system (2) formation one jointly; System auto-control software, date processing and image reconstruction software in described industrial control computer (4) are for realizing automatization's detection of diffuse optical signal and photoacoustic signal, data acquisition and pretreatment and image reconstruction; Wherein, auto-control software for realizing the sequencing contro to diffuse optical chromatography and optoacoustic chromatography combined measurement system components, to realize the automatization of measuring process; Date processing and image reconstruction software are used for carrying out pretreatment to associating measuring system measurement data, and adopt image reconstruction algorithm to carry out image reconstruction to diffused light measurement data and optoacoustic measurement data, thus obtain objective body diffuse optical chromatography and optoacoustic chromatography bimodal combines reconstruction image;
Described diffuse optical laminar analysis measurement part (1) further comprises optical receiving system (11), stable light source system (12), filter system (13) and detection system (14); Described optoacoustic chromatography measure portion (3) further comprises optoacoustic detection system (31), light-pulse generator system (32) and data collecting system (33).
2. the combined measurement system of diffuse optical chromatography as claimed in claim 1 and optoacoustic chromatography, it is characterized in that, described imitative CT spacescan system comprises water tank (21), immerse the electrical-controlled lifting platform and turntable (22) that arrange in water tank, imaging chamber (23) and for carry first ultrasonic transducer (311), second ultrasonic transducer (312) is to realize the automatically controlled annular detection turntable (24) of signal circular scanning, objective body (5) is placed in columniform imaging chamber (23), imaging chamber (23) maintains cylindrical shape and can ensure that ultrasonic signal has effect spread, imaging chamber (23) is fixed on electrical-controlled lifting platform and turntable (22), by the control to lifting spacing and the anglec of rotation, adjust different imaging surface (6) upper Different Light incident angle, i.e. any setting of implementation space sample rate, in described water tank (21), the water of carrying is as ultrasonic coupling liquid, is dipped in coupling liquid by whole scanning system, for the propagation of acoustic signal and the decay of minimizing ultrasonic signal.
3. the combined measurement system of diffuse optical chromatography as claimed in claim 2 and optoacoustic chromatography, it is characterized in that, described diffuse optical laminar analysis measurement part (1) also comprises optical receiving system (11), stable light source system (12), automatically controlled filter system (13) and detection system (14); Wherein:
Described optical receiving system (11) is uniformly distributed 8 detection optical fibers by the first-class angle intervals in same detection plane position of objective body (5) offside and forms, and adopts the semicircle structure of fiber_optic coaxial with imaging chamber to fix;
Described stable light source system (12) comprises two wavelength and is respectively λ
1and λ
2the first stable state semiconductor laser (121), the second stable state semiconductor laser (122), and it is fine to connect axis incident light; Incident illumination vertical incidence after collimator (123) is surperficial to objective body (5); Be linked in the different passages of 8:1 photoswitch (111) respectively, 8:1 photoswitch (111) emergent light is through collimator (112) extremely automatically controlled filter system (13);
Described automatically controlled filter system (13) comprises filter wheel controller (131) and automatically controlled filter wheel (132), and emergent light is incident to detection system (14) by automatically controlled filter system;
Described detection system (14) comprises photomultiplier tube (141) and counting module (142), described photomultiplier tube (141) receiving optical signals also converts the signal of telecommunication to, is undertaken processing and record by counting module (142).
4. the combined measurement system of diffuse optical chromatography as claimed in claim 2 and optoacoustic chromatography, it is characterized in that, described optoacoustic chromatography measure portion (3) also comprises optoacoustic detection system (31), light-pulse generator system (32) and data collecting system (33); Wherein:
Described light-pulse generator system (32) adopts the distributed more incident mode of pulse laser (321) to realize the uniform irradiation of high energy pulse incident illumination to objective body (5) surface, connect one group of both arms fibre bundle (322), 1/4 semicircular domain of fibre bundle transmission laser difference uniform irradiation imaging surface;
Described optoacoustic detection system (31) is made up of first ultrasonic transducer (311) of carry on automatically controlled annular detection turntable (24) and the second ultrasonic transducer (312), and relative incident light source is positioned at reflection measurement face; The circular scanning position of its probe is adjusted by automatically controlled annular detection turntable (24); The photoacoustic signal of objective body (5) outgoing changes into the signal of telecommunication by after the first ultrasonic transducer (311) and the second ultrasonic transducer (312) detection, is sent to data collecting system (33);
Described data collecting system (33) comprises preamplifier (331) and high-speed data acquisition card (332).
5. a union measuring method for diffuse optical chromatography and optoacoustic chromatography, is characterized in that, the method comprises the following steps:
Step (1), the stable light source system of selected wavelength is connected to axis incident light fibre, incident optical after collimator vertical incidence to target surface, 8 detection optical fibers are uniformly distributed in the first-class angle intervals in position, isoplanar of its offside, be linked in the different passages of multichannel optical switch respectively, obtained the emergent light signal of different detecting location by the switching of 8:1 photoswitch; 8:1 photoswitch emergent light is by being incident to photomultiplier tube after automatically controlled filter wheel after collimating device collimation, and photomultiplier tube receiving optical signals also converts the signal of telecommunication to and undertaken processing and record by counting module.
Step (2), pulse laser produce pulse laser as photo-acoustic excitation light source, by both arms fibre bundle, exciting light is sent to the different incoming positions of imaging surface, vertical incidence is to target surface to be measured, excite the photoacoustic signal of generation through ultrasonic coupling liquid by pulse laser, be sent to ultrasonic transducer and be converted into the signal of telecommunication, the signal of telecommunication is after preamplifier, carry out data acquisition by high-speed data acquisition card, the data gathered the most at last send industrial control computer to and process; By the circular scanning position of automatically controlled annular detection turntable adjustment ultrasonic transducer, repeat above measuring process, finally can obtain the photoacoustic signal of the lower objective body outgoing to be measured of different angles projection;
Step (3), obtain the diffuse optical signal under single source incident angle by step (1), and then collect by step (2) photoacoustic signal inspired in objective body to be measured; Utilize the system auto-control software in industrial control computer to regulate the automatically controlled turntable in imitative CT spacescan system, thus obtain new light source incidence angle, repeat step (1) and (2), obtain the diffuse optical signal on a two-dimensional imaging face under any light source incidence angle and photoacoustic signal; Reutilization system auto-control software regulates electrical-controlled lifting platform and turntable, continue to repeat above step, just can obtain the information on any imaging surface of objective body to be measured, get up the information combination on all imaging surfaces just to obtain the three-dimensional information of whole objective body; Send the information of acquisition to date processing and image reconstruction software, finally can obtain the distributed image of optical parametric in two dimension or three dimensions of objective body.
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