CN104865106B - A kind of microbial aerosol sampling apparatus - Google Patents
A kind of microbial aerosol sampling apparatus Download PDFInfo
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- CN104865106B CN104865106B CN201510333192.2A CN201510333192A CN104865106B CN 104865106 B CN104865106 B CN 104865106B CN 201510333192 A CN201510333192 A CN 201510333192A CN 104865106 B CN104865106 B CN 104865106B
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Abstract
The present invention relates to a kind of microbial aerosol sampling apparatus,It includes microbial solution mist generating device,Compressed air gasification installation,Microorganism colloidal sol generating means and sampling vessel,Wherein,It is connected with the front end of microorganism colloidal sol generating means after microbial solution mist generating device and the connection mixing of compressed air gasification installation,Sampling vessel are connected to the end of the microorganism colloidal sol generating means,The present invention utilizes microbial solution mist generating device,Compressed air gasification installation,Microorganism colloidal sol generating means and sampling vessel,By setting quincuncial spray hole and refinement spray nozzle,Ensure that the generation quality of microbial aerosol,Pressure cylinder is risen by setting,Single filter cylinder and tube of pressure-stabilizing,Ensure gaseous mass and precision that the compression of compressed air gasification installation is obtained,It is further ensured that the generation quality of microbial aerosol,Sampled by using sampling film and physiological saline,Ensure that sampling quality and sampling rate,Improve ability in sampling.
Description
Technical field
The present invention relates to a kind of microbial aerosol sampling apparatus, it is related to a kind of sampling of aerosol field, belongs to aerosol
Technical field.
Background technology
Microbial aerosol be a group body it is small, simple structure unicellular or close to single celled biological suspension in sky
Colloidal dispersion formed thereby in gas, at 0.01-100 μm, generally 0.30 μm microbial aerosol has 6 big spies to particle size
Property:The heterogeneity in source, the diversity of species, activity mutability, send out it is three-dimensionality, deposition reproducibility, infection it is wide
General property.Microbial aerosol activity since it formed moment be just constantly in variable condition, the microorganism in air with
Human being's production and life it is closely bound up, it can both promote the well-being of mankind, and can also endanger life and the health of the mankind, therefore, research with
Concentration, species, distribution and its Changing Pattern for monitoring air microbe aerosol are significant.And to microbial aerosol
Research is sampled firstly the need of to it, but the sampling efficiency of current microbial aerosol is low, and the sampling time is long, matter of sampling
Amount is poor, it is impossible to meet quick to microbial aerosol, efficient, high-quality sampling request.
In order to solve the above technical problems, the invention provides a kind of microbial aerosol sampling apparatus, it utilizes micro- life
Thing solution atomization generating means, compressed air gasification installation, microorganism colloidal sol generating means and sampling vessel, by setting plum blossom
The spray hole and refinement spray nozzle of shape, it is ensured that the generation quality of microbial aerosol, pressure cylinder, single filter cylinder are risen by setting
And tube of pressure-stabilizing, it is ensured that gaseous mass and precision that the compression of compressed air gasification installation is obtained, further ensure bacterial gas
The generation quality of colloidal sol, additionally, being sampled by using sampling film and physiological saline, it is ensured that sampling quality and sampling speed
Rate, improves ability in sampling.
The content of the invention
To achieve the above object, the present invention is adopted the following technical scheme that:A kind of microbial aerosol sampling apparatus, its feature
It is that it includes microbial solution mist generating device, compressed air gasification installation, microorganism colloidal sol generating means and sampler
Ware, wherein, with micro- life after the microbial solution mist generating device and compressed air gasification installation connection mixing
The front end connection of thing colloidal sol generating means, the sampling vessel are connected to the end of the microorganism colloidal sol generating means.
Further, preferably, the microbial solution mist generating device includes air pump, microbial solution bottle, sprinkling
Mouth, refinement spray nozzle and pressure maintaining valve, wherein, the air pump is connected by conduit with the solution in microbial solution bottle, institute
The side for stating microbial solution bottle is provided with the spray nozzle, intensive in the spray nozzle to be provided with multiple cross sections for quincunx
Spray hole, and the profile of the spray nozzle is cone, and the spray nozzle is connected with the refinement spray nozzle, institute by conduit
State and the pressure maintaining valve is additionally provided with the conduit between air pump and the microbial solution;The compressed air gasification installation includes
Blower fan, liter pressure cylinder, single filter cylinder and tube of pressure-stabilizing, wherein, the outlet side of the blower fan is connected with one end of described liter of pressure cylinder, institute
The other end for stating liter pressure cylinder is connected with single filter cylinder, and the single filter cylinder is connected with the tube of pressure-stabilizing, the one-level
Cartridge filter is internally provided with multiple filtration core, and described liter of pressure cylinder is shaped as conical stainless steel cylinder, and the tapered end of cone with
The single filter cylinder connection, the single filter cylinder and the tube of pressure-stabilizing are the stainless steel cylinder of cylinder;Described liter of pressure cylinder
Sealing gasket and threaded connection, institute are used between single filter cylinder, between single filter cylinder and the tube of pressure-stabilizing
State microorganism colloidal sol generating means and cylinder and microbial aerosol stabilizing sleeve occur including microbial aerosol, wherein, the refinement
What spray nozzle was stretched into is arranged on inside the microbial aerosol generation cylinder, and the microbial aerosol occurs cylinder and micro- life
Thing aerosol stabilizing sleeve is connected, and the microbial aerosol stabilizing sleeve is internally provided with filter membrane, the microbial aerosol hair
Raw cylinder and microbial aerosol stabilizing sleeve are glass transparent cylindrical drum;It is described sampling vessel include sampling glass box, vavuum pump,
Filter and globe valve, wherein, the sampling glass box is connected with the aerosol stabilizing sleeve, is set inside the sampling glass box
Sampling film is equipped with, vavuum pump is also associated with the sampling glass box, the vavuum pump is connected with the filter, the sampling
Physiological saline is additionally provided with inside glass box.
Further, preferably, the thickness of the stainless steel cylinder of single filter cylinder and the tube of pressure-stabilizing is 1-2mm.
Further, preferably, the sampling glass box is shaped as inverted cone, and the cone lower end
Opening is provided with, the opening is using rubber stopper closure.
Further, preferably, the blower fan of the compressed air gasification installation be centrifugal fan, and blower fan power not
Less than 1KW, air quantity is not less than 1200 cubes ms/h.
Further, preferably, being additionally provided with temperature sensor and flow sensing in the microorganism colloidal sol generating means
Device.
Further, preferably, the sampling film used in vessel is multilayer, and the thickness of sampling film is 0.2-
0.3mm。
Further, preferably, the cross section of the spray hole of spray nozzle in the microbial solution mist generating device
Area is 0.1-0.2mm2, the cross-sectional area of the spray holes of the refinement spray nozzle in the microbial solution mist generating device is
0.02-0.06mm2。
The beneficial effects of the present invention are:
A kind of microbial aerosol sampling apparatus that the present invention is provided, it utilizes microbial solution mist generating device, pressure
Contracting air gasification device, microorganism colloidal sol generating means and sampling vessel, are sprayed by setting quincuncial spray hole and refinement
Mouth, it is ensured that the generation quality of microbial aerosol, rises pressure cylinder, single filter cylinder and tube of pressure-stabilizing, it is ensured that compression by setting
Gaseous mass and precision that the compression of air gasification device is obtained, further ensure the generation quality of microbial aerosol, additionally,
Sampled by using sampling film and physiological saline, it is ensured that sampling quality and sampling rate, improve ability in sampling.
Brief description of the drawings
Fig. 1 is a kind of structural representation of microbial aerosol sampling apparatus of the invention;
Wherein, 1, air pump, 2, microbial solution bottle, 3, spray nozzle, 4, refinement spray nozzle, 5, pressure maintaining valve, 6, blower fan, 7, rise
Pressure cylinder, 8, single filter cylinder, 9, tube of pressure-stabilizing, 10, filtration core, 11, microbial aerosol there is cylinder, 12, microbial aerosol it is steady
Safety barrel, 13, sampling glass box, 14, vavuum pump, 15, filter, 16, globe valve, 17, sampling film.
Specific embodiment
Detailed description is carried out to the present invention below in conjunction with accompanying drawing.It should be appreciated, however, that accompanying drawing has been provided only more
Understand the present invention well, they should not be interpreted as limitation of the present invention.
As shown in figure 1, the invention provides a kind of microbial aerosol sampling apparatus, it is characterised in that it includes micro- life
Thing solution atomization generating means, compressed air gasification installation, microorganism colloidal sol generating means and sampling vessel, wherein, it is described micro-
With the microorganism colloidal sol generating means after biological solution mist generating device and compressed air gasification installation connection mixing
Front end connection, the sampling vessel are connected to the end of the microorganism colloidal sol generating means.
In the present embodiment, microbial solution mist generating device includes air pump 1, microbial solution bottle 2, spray nozzle 3, thin
Change spray nozzle 4 and pressure maintaining valve 5, wherein, air pump 1 is connected by conduit with the solution in microbial solution bottle 2, and microorganism is molten
The side of liquid bottle 2 is provided with the spray nozzle, intensive in spray nozzle 3 to be provided with multiple cross sections for quincuncial spray hole, and
The profile of spray nozzle 3 is cone, and spray nozzle 3 is connected with the refinement spray nozzle 4 by conduit, and the air pump 1 is micro- with described
The pressure maintaining valve 5 is additionally provided with conduit between biological solution 2;The compressed air gasification installation includes blower fan 6, rises pressure cylinder
7th, single filter cylinder 8 and tube of pressure-stabilizing 9, wherein, the outlet side of blower fan 6 is connected with one end of described liter of pressure cylinder 7, rises the another of pressure cylinder 7
One end is connected with single filter cylinder 8, and single filter cylinder 8 is connected with the tube of pressure-stabilizing 9, and single filter cylinder 8 is internally provided with
Multiple filtration core 10, described liter of pressure cylinder 7 is shaped as conical stainless steel cylinder, and the tapered end of cone and single filter cylinder
8 connections, single filter cylinder ε and the tube of pressure-stabilizing 9 are the stainless steel cylinder of cylinder;Rise pressure cylinder and the single filter cylinder
Between, single filter cylinder and the tube of pressure-stabilizing between use sealing gasket and threaded connection, microorganism colloidal sol generating means include it is micro-
There is cylinder 11 and microbial aerosol stabilizing sleeve 12 in bioaerosol, wherein, what refinement spray nozzle 4 was stretched into is arranged on micro- life
Thing aerosol occurs inside cylinder 11, and microbial aerosol occurs cylinder 11 and is connected with the microbial aerosol stabilizing sleeve 12, micro- life
Thing aerosol stabilizing sleeve 12 is internally provided with filter membrane (not shown), and microbial aerosol occurs cylinder 11 and bacterial gas are molten
Glue stabilizing sleeve 12 is glass transparent cylindrical drum;The sampling vessel include sampling glass box 13, vavuum pump 14, the and of filter 15
Globe valve 16, wherein, sampling glass box 13 is connected with the aerosol stabilizing sleeve 12, and sampling glass box is internally provided with sampling film
17, vavuum pump 14 is also associated with sampling glass box 13, vavuum pump 14 is connected with the filter 15, inside sampling glass box 13
It is additionally provided with physiological saline.
In the present embodiment, the thickness of the stainless steel cylinder of single filter cylinder and the tube of pressure-stabilizing is 1-2mm.Sampling glass box
Be shaped as inverted cone, and the lower end of the cone is provided with opening, and the opening is blocked using rubber stopper.Compression
The blower fan of air gasification device be centrifugal fan, and blower fan power be not less than 1KW, air quantity be not less than 1200 cubic metres/it is small
When.
For the ease of being controlled to temperature and flow, be additionally provided with microorganism colloidal sol generating means temperature sensor and
Flow sensor.Sampling film in sampling vessel is multilayer, and the thickness of sampling film is 0.2-0.3mm.Microbial solution is atomized
The cross-sectional area of the spray hole of the spray nozzle in generating means is 0.1-0.2mm2, in microbial solution mist generating device
The cross-sectional area for refining the spray holes of spray nozzle is 0.02-0.06mm2。
The present invention is using microbial solution mist generating device, compressed air gasification installation, microorganism colloidal sol generating means
With sampling vessel, by setting quincuncial spray hole and refinement spray nozzle, it is ensured that the generation quality of microbial aerosol, lead to
Cross setting and rise pressure cylinder, single filter cylinder and tube of pressure-stabilizing, it is ensured that gaseous mass and essence that the compression of compressed air gasification installation is obtained
Degree, further ensures the generation quality of microbial aerosol, additionally, sampled by using sampling film and physiological saline,
Sampling quality and sampling rate are ensure that, ability in sampling is improve.
Embodiment of above is merely to illustrate the present invention, and not limitation of the present invention, about the common of technical field
Technical staff, without departing from the spirit and scope of the present invention, can also make a variety of changes and modification, therefore all
Equivalent technical scheme falls within scope of the invention, and scope of patent protection of the invention should be defined by the claims.
Claims (7)
1. a kind of microbial aerosol sampling apparatus, it is characterised in that it includes that microbial solution mist generating device, compression are empty
Gas gasification installation, microorganism colloidal sol generating means and sampling vessel, wherein, the microbial solution mist generating device and described
It is connected with the front end of the microorganism colloidal sol generating means after the connection mixing of compressed air gasification installation, the sampling vessel connection
In the end of the microorganism colloidal sol generating means, the microbial solution mist generating device includes air pump, microbial solution
Bottle, spray nozzle, refinement spray nozzle and pressure maintaining valve, wherein, the air pump is by the solution in conduit and the microbial solution bottle
Connection, the side of the microbial solution bottle is provided with the spray nozzle, intensive in the spray nozzle to be provided with multiple cross sections
It is quincuncial spray hole, and the profile of the spray nozzle is cone, the spray nozzle is connected with the refinement by conduit
Spray nozzle, is additionally provided with the pressure maintaining valve on the conduit between the air pump and the microbial solution;The compressed air gas
Makeup is put including blower fan, rises pressure cylinder, single filter cylinder and tube of pressure-stabilizing, wherein, the outlet side of the blower fan and described liter of pressure cylinder
One end connects, and the described liter of other end of pressure cylinder is connected with single filter cylinder, and the single filter cylinder connects with the tube of pressure-stabilizing
Connect, single filter cylinder is internally provided with multiple filtration core, described liter of pressure cylinder is shaped as conical stainless steel cylinder, and circular cone
The tapered end of shape is connected with single filter cylinder, and the single filter cylinder and the tube of pressure-stabilizing are cylindrical stainless steel cylinder;
Sealing gasket and spiral shell are used between described liter of pressure cylinder and single filter cylinder, between single filter cylinder and the tube of pressure-stabilizing
Line is connected, and the microorganism colloidal sol generating means include that microbial aerosol occurs cylinder and microbial aerosol stabilizing sleeve, wherein,
Refinement being arranged on of stretching into of the spray nozzle microbial aerosol occurs inside cylinder, the microbial aerosol occur cylinder with
The microbial aerosol stabilizing sleeve connection, the microbial aerosol stabilizing sleeve is internally provided with filter membrane, the microorganism
There is cylinder in aerosol and microbial aerosol stabilizing sleeve is glass transparent cylindrical drum;The sampling vessel include sampling glass
Case, vavuum pump, filter and globe valve, wherein, the sampling glass box is connected with the aerosol stabilizing sleeve, the sampling glass
Glass case is internally provided with sampling film, and vavuum pump is also associated with the sampling glass box, and the vavuum pump connects with the filter
Connect, globe valve is provided with the filter, physiological saline is additionally provided with inside the sampling glass box.
2. a kind of microbial aerosol sampling apparatus according to claim 1, it is characterised in that the single filter cylinder and
The thickness of the stainless steel cylinder of the tube of pressure-stabilizing is 1-2mm.
3. a kind of microbial aerosol sampling apparatus according to claim 1, it is characterised in that the sampling glass box
Inverted cone is shaped as, and the lower end of the cone is provided with opening, the opening is using rubber stopper closure.
4. a kind of microbial aerosol sampling apparatus according to claim 1, it is characterised in that the compressed air gasification
The blower fan of device is centrifugal fan, and the power of blower fan is not less than 1KW, and air quantity is not less than 1200 cubes ms/h.
5. a kind of microbial aerosol sampling apparatus according to claim 1, it is characterised in that the microorganism colloidal sol life
Temperature sensor and flow sensor are additionally provided with into device.
6. a kind of microbial aerosol sampling apparatus according to claim 1-5 any one, it is characterised in that described to adopt
Sampling film in sample vessel is multilayer, and the thickness of sampling film is 0.2-0.3mm.
7. a kind of microbial aerosol sampling apparatus according to claim 1-5 any one, it is characterised in that described micro-
The cross-sectional area of the spray hole of the spray nozzle in biological solution mist generating device is 0.1-0.2mm2, the microbial solution
The cross-sectional area of the spray holes of the refinement spray nozzle in mist generating device is 0.02-0.06mm2。
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CN105385585B (en) * | 2015-11-16 | 2017-12-26 | 安徽瑞达健康产业投资有限公司 | A kind of microbe filter material property detection means |
CN106442025A (en) * | 2016-10-17 | 2017-02-22 | 中国科学院深圳先进技术研究院 | Air microorganism sampling device |
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GB2566453B (en) * | 2017-09-12 | 2021-01-13 | Univ Of Northumbria At Newcastle | Impactor for aerosol component collection |
CN109266524B (en) * | 2018-09-21 | 2021-07-06 | 中国科学院生态环境研究中心 | Closed microorganism aerosol generating device |
CN109854956B (en) * | 2018-12-29 | 2024-02-27 | 江苏苏净集团有限公司 | High-pressure gas diffuser for sampling |
CN111781015B (en) * | 2020-07-30 | 2023-07-21 | 中国检验检疫科学研究院 | Optimization method and device for sampling efficiency of gas-liquid mixing biological aerosol collecting device |
CN116793767B (en) * | 2023-05-19 | 2024-01-26 | 夏津新希望六和农牧有限公司 | Active capturing device for African swine fever aerosol and detection method thereof |
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