CN104855258A - Hormone treatment method for improving water culture rooting of carpinus betulus - Google Patents
Hormone treatment method for improving water culture rooting of carpinus betulus Download PDFInfo
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- CN104855258A CN104855258A CN201510203382.2A CN201510203382A CN104855258A CN 104855258 A CN104855258 A CN 104855258A CN 201510203382 A CN201510203382 A CN 201510203382A CN 104855258 A CN104855258 A CN 104855258A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention discloses a hormone treatment method for improving water culture rooting of carpinus betulus. The method comprises the following steps: selecting hard stem segments growing for 2-3 years; cutting the upper ends of the stem segments into pain ends and chamfering the lower ends to ensure the length of the stem segments between 8-12 cm; reserving two half leaves; washing the incisions with tap water and soaking the stem segments in a carbendazim solution with the concentration of 0.1 %-0.3 % for 20-30 min to disinfect and sterilize the stem segments; inserting the sterilized branches in ABT1 with the concentration of 600-800 mg/L for 1-2 h; inserting the branches with the hormone treated in tap water and keeping the air humidity in the culture room over 85 % two weeks before water culture and 70 %-80 % two weeks after water culture, and keeping the temperature at 24-26 DEG C; spraying the carbendazim solution with the concentration of 0.1 %-0.3 % on the surfaces of the branches every week; changing water every two weeks; carrying out statistics of the survival rate, the rooting rate and the callus tissue rate after 55 days. The method has the advantage that the hydroponic carpinus betulus is ensured to be higher in survival rate, rooting rate and callus tissue formation rate.
Description
Technical field
What the present invention relates to is a kind of hormone treatment method improving Carpinus betulus water planting and take root, and is induced the generation of aquatic root system and callus, finally grow into the method for a strain whole plant by the mode of HORMONE TREATMENT hard lopwood bar.Belong to Ornamental Plants and Horticulture to learn a skill field.
Background technology
Water culture rooting propagation is exactly do " matrix " cottage propagation flowers and trees with water, and makes it to produce the method that new root becomes Individual plant body, is now widely used on vegetables and flowers class plant.The research of taking root to woody plant water planting in a large number shows some water culture cuttages, and comparatively matrix cutting experiment is good, and as more native in Water culture such as gardenia, fig, the rose of Sharons inserting is taken root ahead of time; Masson pine IBA water bed Rooting percent is higher; Hypericum perforatum plantlet in vitro water planting rootability is strong, fast growth.Compare and directly plant is taken out from soil or matrix, clean root system, enter the method for Aquaponic, use branch and be more conducive to the formation of root system aerenchyma by hormone-treated water planting mode, be also conducive to improving reproduction coefficient.
Carpinus betulus (
carpinus betulus)for Betulaceae Carpinus broad leaved and deciduous broad leaved arbor, originate in Europe and West Asia.Tree crown is plentiful, autumn leaves is golden yellow, has stronger adaptability, more cold-resistant, happiness calcium, wind resistance, is widely used as street tree, garden tree, hedgerow and potted landscape etc., has wide market prospects in many countries and regions.But Carpinus betulus seed has dormant trait, cottage propagation rootage duration is long, and rooting rate is not high, and summer, cuttage was easily polluted, and is unfavorable for the large-scale production of Carpinus betulus.Water planting breeding has the feature of high-efficiency cleaning, and there is not yet the report about the cultivation of Carpinus betulus water planting both at home and abroad, and the investigation and application for Carpinus betulus water planting root-promoting hormone processing mode has the larger market space.
Summary of the invention
What the present invention proposed is a kind of hormone treatment method improving Carpinus betulus water planting and take root, and its objective is the survival rate, rooting rate and the Callus formation rate that improve Carpinus betulus, and after formation water planting root system and callus, energy long term growth is in water environment.
Technical solution of the present invention, a kind of hormone treatment method improving Carpinus betulus water planting and take root, comprises the following steps:
1) branch collection, select 2 ~ 3 years stiff branch stem sections, upper end is cut into flat mouth, cuts sth. askew in lower end, and long 8 ~ 12cm, retains 2 1/2 leaves, adopt rear tap water otch, and soaks 20 ~ 30min disinfection with the carbendazim solution of 0.1% ~ 0.3%;
2) HORMONE TREATMENT, inserts the branch of having sterilized in the ABT1 solution of 600 ~ 800mg/L and soaks 1 ~ 2h, ensures that the branch in distance otch 3cm is fully immersed in solution;
3) culture technique, branch HORMONE TREATMENT crossed inserts in running water, the degree of depth 1 ~ 3cm, before ensureing the inherent water planting of culturing room, 2 weeks air humiditys are not less than 85%, after 2 weeks, air humidity remains on 70% ~ 80%, temperature controls at 24 ~ 26 DEG C, and use weekly the carbendazim solution of 0.1% ~ 0.3% to spray branch surface, every 2 Zhou Huanshui once;
4) survival rate, rooting rate, callus rate is added up after 55 days, survival rate=(water planting retains total branch number of the branch number/water planting of blade) × 100%; Rooting rate=(total branch number of branch number/water planting that water planting is taken root) × 100%; Callus rate=(water planting forms total branch number of the branch number/water planting of callus) × 100%.
Advantage of the present invention:
1) Carpinus betulus is American-European conventional Landscape Tree Species, at home and abroad adopt seminal propagation, cottage propagation,
Propagation by grafiting and tissue culture propagation, do not see the report of water planting breeding.And water culture technology has, and sight is strong, maintenance is convenient, clean few feature such as damage by disease and insect, Economization on land, not only can be applicable to ornamental plantation but also can be applicable to indoor greening, also abatement functions is had to water pollutions, therefore, active development Carpinus betulus water planting propagation technique can be optimized its mode of production undoubtedly and open up multifunctional application, for domestic nursery stock markets of viewing and admiring provides a kind of novel seedling.Therefore, Carpinus betulus water planting rooting technique achievement has important innovative significance;
2) the different plant inducer water guide ability of taking root is different, some kind to HORMONE TREATMENT mode require strict very
To not needing hormone induction can form water root, as fairy bamboo.But research shows that the water planting of Carpinus betulus needs certain HORMONE TREATMENT to form water root, and the rooting rate of its clear water process is zero.And different HORMONE TREATMENT modes causes different rooting rates, survival rate and callus rate, the HORMONE TREATMENT mode in the present invention can improve the rooting rate of Carpinus betulus, survival rate and callus rate, has great significance to its factorial praluction.Compare with the external HORMONE TREATMENT mode to Carpinus betulus cottage propagation, the hormone kind of water planting, hormone concentration and soak time all have innovation.Therefore, the HORMONE TREATMENT mode be based upon in achievement in research is one of key point of the present invention.
Embodiment
Below embodiments of the invention are described in further detail:
Embodiment 1
Improve the hormone treatment method that Carpinus betulus water planting is taken root, it adopts following steps:
Branch gathers, and select 2 years stiff branch stem sections, upper end is cut into flat mouth, cuts sth. askew in lower end, long 10cm, retains 2 1/2 leaves.Adopt rear tap water otch, and soak 30min disinfection with the carbendazim solution of 0.1%.
HORMONE TREATMENT, inserts the branch of having sterilized in the ABT1 solution of 600mg/L and soaks 2h, ensures that the branch in distance otch 3cm is fully immersed in solution.
Culture technique, branch HORMONE TREATMENT crossed inserts in running water, degree of depth 2cm, and before ensureing the inherent water planting of culturing room, 2 weeks air humiditys are not less than 85%, and after 2 weeks, air humidity remains on 70%, and temperature controls at 25 DEG C.Use weekly the carbendazim solution of 0.1% to spray branch surface, every 2 Zhou Huanshui once.
Survival rate, rooting rate, callus rate is added up after 55 days.Survival rate=(water planting retains total branch number of the branch number/water planting of blade) × 100%; Rooting rate=(total branch number of branch number/water planting that water planting is taken root) × 100%; Callus rate=(water planting forms total branch number of the branch number/water planting of callus) × 100%.
embodiment 2
Branch gathers, and select 3 years stiff branch stem sections, upper end is cut into flat mouth, cuts sth. askew in lower end, long 8cm, retains 2 1/2 leaves.Adopt rear tap water otch, and soak 20min disinfection with the carbendazim solution of 0.3%.
HORMONE TREATMENT, inserts the branch of having sterilized in the ABT1 solution of 700mg/L and soaks 2h, ensures that the branch in distance otch 3cm is fully immersed in solution.
Culture technique, branch HORMONE TREATMENT crossed inserts in running water, degree of depth 3cm, and before ensureing the inherent water planting of culturing room, 2 weeks air humiditys are not less than 85%, and after 2 weeks, air humidity remains on 80%, and temperature controls at 25 DEG C.Use weekly the carbendazim solution of 0.3% to spray branch surface, every 2 Zhou Huanshui once.
Survival rate, rooting rate, callus rate is added up after 55 days.Survival rate=(water planting retains total branch number of the branch number/water planting of blade) × 100%; Rooting rate=(total branch number of branch number/water planting that water planting is taken root) × 100%; Callus rate=(water planting forms total branch number of the branch number/water planting of callus) × 100%.
embodiment 3
Branch gathers, and select 2 years stiff branch stem sections, upper end is cut into flat mouth, cuts sth. askew in lower end, long 12cm, retains 2 1/2 leaves.Adopt rear tap water otch, and soak 20min disinfection with the carbendazim solution of 0.1%.
HORMONE TREATMENT, inserts the branch of having sterilized in the ABT1 solution of 800mg/L and soaks 1h, ensures that the branch in distance otch 3cm is fully immersed in solution.
Culture technique, branch HORMONE TREATMENT crossed inserts in running water, degree of depth 3cm, and before ensureing the inherent water planting of culturing room, 2 weeks air humiditys are not less than 85%, and after 2 weeks, air humidity remains on 75%, and temperature controls at 25 DEG C.Use weekly the carbendazim solution of 0.3% to spray branch surface, every 2 Zhou Huanshui once.
Survival rate, rooting rate, callus rate is added up after 55 days.Survival rate=(water planting retains total branch number of the branch number/water planting of blade) × 100%; Rooting rate=(total branch number of branch number/water planting that water planting is taken root) × 100%; Callus rate=(water planting forms total branch number of the branch number/water planting of callus) × 100%.
Contrast and each rooting inhibitors statistical form of embodiment Carpinus betulus
Result shows, use the ABT1 HORMONE TREATMENT of 600 ~ 800mg/L can significantly improve the indices of Carpinus betulus water planting, wherein the rooting rate of Carpinus betulus water planting is brought up to about 60% by 0% of clear water control group by ABT1 HORMONE TREATMENT, survival rate rises about 22% than clear water control group, and callus rate rises about 17% than clear water control group.
Claims (4)
1. improve the hormone treatment method that Carpinus betulus water planting is taken root, it is characterized in that, the method comprises the following steps:
1) branch collection, disinfection; Select 2 ~ 3 years stiff branch stem sections, upper end is cut into flat mouth, cuts sth. askew in lower end, and long 8 ~ 12cm, retains 2 1/2 leaves, adopt rear tap water otch, and soaks 20 ~ 30min disinfection with the carbendazim solution of 0.1% ~ 0.3%;
2) HORMONE TREATMENT, inserts the branch of having sterilized in the ABT1 solution of 600 ~ 800mg/L and soaks 1 ~ 2h, ensures that the branch in distance otch 3cm is fully immersed in solution;
3) culture technique, branch HORMONE TREATMENT crossed inserts in running water, the degree of depth 1 ~ 3cm, and before ensureing the inherent water planting of culturing room, 2 weeks air humiditys are not less than 85%, and after 2 weeks, air humidity remains on 70% ~ 80%, and temperature controls at 24 ~ 26 DEG C;
4) survival rate, rooting rate, callus rate is added up after 55 days, survival rate=(water planting retains total branch number of the branch number/water planting of blade) × 100%; Rooting rate=(total branch number of branch number/water planting that water planting is taken root) × 100%; Callus rate=(water planting forms total branch number of the branch number/water planting of callus) × 100%.
2. a kind of hormone treatment method improving Carpinus betulus water planting and take root according to claim 1, is characterized in that described branch collection, and select 2 ~ 3 years stiff branch stem sections, upper end is cut into flat mouth, cuts sth. askew in lower end, and long 8 ~ 12cm, retains 2 1/2 leaves.
3. a kind of hormone treatment method improving Carpinus betulus water planting and take root according to claim 1, is characterized in that described disinfection tap water otch, and soaks 20 ~ 30min with the carbendazim solution of 0.1% ~ 0.3%.
4. a kind of hormone treatment method improving Carpinus betulus water planting and take root according to claim 1, is characterized in that described culture technique, and use weekly the carbendazim solution of 0.1% ~ 0.3% to spray branch surface, every 2 Zhou Huanshui once.
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Citations (3)
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| CN101473736A (en) * | 2008-05-18 | 2009-07-08 | 姚成义 | Cuttage and breeding method of Aquilaria sinensis |
| CN103141312A (en) * | 2013-04-03 | 2013-06-12 | 南京林业大学 | Grafting and seedling raising method for European hornbeam |
| CN103181325A (en) * | 2013-04-01 | 2013-07-03 | 南京林业大学 | Tissue culture rapid propagation system of European hornbeam |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101473736A (en) * | 2008-05-18 | 2009-07-08 | 姚成义 | Cuttage and breeding method of Aquilaria sinensis |
| CN103181325A (en) * | 2013-04-01 | 2013-07-03 | 南京林业大学 | Tissue culture rapid propagation system of European hornbeam |
| CN103141312A (en) * | 2013-04-03 | 2013-06-12 | 南京林业大学 | Grafting and seedling raising method for European hornbeam |
Non-Patent Citations (5)
| Title |
|---|
| V.CHALUPA: "Micropropagation of hornbeam (Carpinus betulus L.) and ash (Fraxinus excelsior L.)", 《BIOLOGIA PLANTARUM(PRAHA)》 * |
| 中共青海省委宣传部: "《青海农牧民基本常识读本》", 31 December 2000, 中共青海省委宣传部 * |
| 主要珍稀濒危树种繁殖技术编辑委员会: "《主要珍稀濒危树种繁殖技术》", 30 June 1992, 中国林业出版社 * |
| 祝遵凌等: "欧洲鹅耳枥繁殖技术研究", 《安徽农业大学学报》 * |
| 邱智敏等: "天台鹅耳枥育苗试验初报", 《福建林业科技》 * |
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Effective date of registration: 20180209 Address after: 210008 Yongcheng Road, east of Yongcheng Road, Gaochun Economic Development Zone, Nanjing, Jiangsu Province, south of Dongxu Road Patentee after: Nanjing state environmental protection Mstar Technology Ltd Address before: Nanjing City, Jiangsu province 210037 Longpan Road No. 159 Patentee before: Nanjing Forestry University |