CN104844812A - Method for preparing composite active edible film by utilizing shelled shrimp processed leftovers - Google Patents
Method for preparing composite active edible film by utilizing shelled shrimp processed leftovers Download PDFInfo
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- CN104844812A CN104844812A CN201510262176.9A CN201510262176A CN104844812A CN 104844812 A CN104844812 A CN 104844812A CN 201510262176 A CN201510262176 A CN 201510262176A CN 104844812 A CN104844812 A CN 104844812A
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- processing fent
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Abstract
The invention discloses a method for preparing a composite active edible film by utilizing shelled shrimps processed leftovers. The method comprises the technical processes of: preparing egg white in the shelled shrimps processed leftovers; preparing enzymolysis polypeptide in the shelled shrimps processed leftovers; preparing chitosan in the shelled shrimps processed leftovers; preparing film solution from the prepared egg white, enzymolysis polypeptide and chitosan in the shelled shrimps processed leftover, an antioxidant and a plasticizer; finally, pouring the film solution which is successfully prepared on a polyvinyl chloride crystal board to carry out scraping, drying and film tearing to prepare the composite active edible film. According to the invention, the shelled shrimps processed leftovers are used as main raw materials and a new approach is explored for high-valued utilization of the leftovers; the prepared composite active edible film has the obvious characteristics of rich nutrition, high oxidation resistance and the like and has wide market application prospect in the field of food packaging.
Description
Technical field
The invention belongs to food processing field, relate to a kind of preparation method of edible packing membrane, particularly relate to the method utilizing peeled shrimp processing fent to prepare composite reactive edible film.
Background technology
Edible film, be with edible natural material (as polysaccharide, albumen, lipid) for raw material, utilize certain processing method by the interaction between differing molecular formed a kind of there is excellent mechanical strength and selective penetrated property compact structure, can the edible film of Some substitute Plastic Packaging Materials.Edible film can be covered in food surfaces (or inner) with forms such as parcel, coating, microcapsule, thus reaches the infiltration stoping aqueous vapor, oxygen or various solute, improves Food Quality or Shelf-life.In recent years, exploitation for edible film has no longer been confined to the surrogate as plastics film, but its more functional performance is given under guarantee basic function prerequisite, as in edible film production process, add the materials such as antiseptic-germicide, antioxidant, pigment, flavour substances, nutrition-fortifying agent, spice.Therefore, plyability active deible film will be development trend from now on.
In recent years, China's peeled shrimp output and the total volume of exports are continuing to increase, and peeled shrimp is in the course of processing, create the tankage such as shrimp head, shrimp shell accounting for shrimp total mass 30% ~ 40%, and wherein part is used to produce feed.Other parts are then dropped process, i.e. contaminate environment, ample resources also can be caused to waste simultaneously.Now there are some researches show, containing a large amount of active substance, wherein protein and polysaccharide (chitin) rich content especially in shrimping beam trawl, if reasonable development, will larger economic benefit be produced.
Based on this, applicant, according to the present Research of edible film and peeled shrimp processing fent, with peeled shrimp processing fent for raw material, by series of processes process, prepares a kind of composite reactive edible film.
Summary of the invention
The object of the present invention is to provide and a kind ofly significantly improve shrimps processing added value, reduce aquatic products processing waste to the pollution of environment, be easy to digest, nutrition reinforced effects is good, can the peeled shrimp processing fent that utilize of Shelf-life prepare the method for composite reactive edible film.
For achieving the above object, technical scheme of the present invention is: utilize peeled shrimp processing fent to prepare the method for composite reactive edible film, it prepares albumen, enzymolysis polypeptide and chitosan in peeled shrimp processing fent by certain technique, then in albumen and chitosan solution, enzymolysis polypeptide, tea-polyphenol and softening agent is added, obtain composite reactive edible film after drying and moulding, concrete steps are as follows:
(1) preparation of albumen in peeled shrimp processing fent:freezing shrimp meat processing fent (shrimp head and shrimp shell) is pulverized with tissue mashing machine, phosphate buffered saline buffer (0.02 mol/L is added according to solid-liquid ratio 1 g:2 ~ 3mL, pH 6.5 ~ 7.5), stir extraction 5 ~ 6 h at 35 ~ 40 DEG C after, in 85 ~ 90 DEG C of insulation 10 ~ 15 min, centrifugal 25 ~ 30 min of 8 000 r/min, get in supernatant liquor and add ethyl acetate solution according to volume ratio 1:1, after concussion 3 ~ 5 min, leave standstill 60 ~ 90 min, taking off a layer aqueous phase molecular weight cut-off is that after 1 kDa dialysis tubing desalination, freeze-drying obtains peeled shrimp processing fent albumen.
peeled shrimp processing fentenzymolysis
the preparation of polypeptide:the centrifugal sediment got in step (1) adds Gly-NaOH damping fluid (0.05 mol/L, pH 9.0 ~ 10.0) according to solid-liquid ratio 1 g:3 ~ 5mL, adds Sumizyme MP (enzyme activity>=2.0 × 10 according to 0.8 ~ 1.0% of weight of precipitate
5u/g), in 45 ~ 55 DEG C of insulation 4 ~ 6 h, after 95 DEG C of insulation 10 min, centrifugal 15 ~ 20 min of 10 000 r/min, supernatant liquor adopts molecular weight cut-off to be that after 100 Da dialysis tubing desalinations, freeze-drying obtains peeled shrimp processing fent enzymolysis polypeptide.
the preparation of chitosan in peeled shrimp processing fent:the centrifugal sediment got in step (2) is washed till pH 6.5 ~ 7.5, then saturated ethylenediamine tetraacetic acid (EDTA) (EDTA) solution 20 ~ 24 h is added according to solid-liquid ratio 1 g:3 ~ 5mL, centrifuging and taking precipitation is washed till pH 6.5 ~ 7.5, then in precipitation, 1.0 mol/L NaOH are added, 25 ~ 30 h are processed at 60 DEG C, centrifuging and taking precipitation is washed till pH 6.5 ~ 7.5, dry chitosan.
the preparation of composite reactive edible film film-forming soln: chitosan prepared by albumen step (1) prepared respectively according to solid-liquid ratio 1g:40 ~ 50 mL and step (3) joins (wherein the ratio 2:1 ~ 1:2 of albumen and chitosan) in the lactic acid solution of 0.20 mol/L, and in solution, add 0.1 ~ 0.3% peeled shrimp processing fent enzymolysis polypeptide, 0.3 ~ 0.5% antioxidant, 0.5 ~ 1.0% softening agent according to weightmeasurement ratio, utilize defoamer to carry out deaeration after stirring, make film-forming soln.
the preparation of composite reactive edible film:be poured on equably by the film-forming soln modulated on polyvinyl chloride crystal slab and carry out striking film forming, in 40 ~ 50% times dryings of room temperature relative humidity after 1 ~ 2 day, namely demoulding obtains peeled shrimp processing fent composite reactive edible film.
As improvement, shrimp described in step (1) be Chinese pipe whip shrimp (
solenocera melantho).
As improvement, described in step (4), antioxidant is tea-polyphenol.
As improvement, described in step (4), softening agent is sorbyl alcohol.
Compared with prior art, the invention has the advantages that:
1, the present invention utilizes peeled shrimp processing fent for raw material, both can significantly improve the added value of shrimps processing, can reduce again the pollution of aquatic products processing waste to environment.
2, the composite reactive edible film that prepared by the present invention contains peeled shrimp processing fent enzymolysis polypeptide, and this enzymolysis polypeptide molecular weight is little, is easy to digest and assimilate, and significantly can strengthen the trophic function of edible film.
3, the composite reactive edible film that prepared by the present invention contains tea-polyphenol, can suppress by UV-induced food lipid oxidation, extends the shelf-lives of food.
4, the shrimps processing fent used in the present invention is food raw material, the process safety preparing albumen, polypeptide and chitosan is reliable, the additives such as the tea-polyphenol added and sorbyl alcohol are all the foodstuff additive that country allows to use, and therefore remain the edibility of this composite reactive film.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail.
A preparation method for peeled shrimp processing fent composite reactive edible film, preparation technology's flow process is as follows: peeled shrimp processing fent → Protein Extraction → residual protein enzymolysis → chitosan extraction → film-forming soln preparation → composite reactive edible film.
Embodiment:
(1) preparation of albumen in peeled shrimp processing fent:freezing shrimp meat processing fent (shrimp head and shrimp shell) is pulverized with tissue mashing machine, phosphate buffered saline buffer (0.02 mol/L, pH 7.0) is added, stir extraction 6 h at 37 DEG C after according to solid-liquid ratio 1 g:3mL, in 85 DEG C of insulation 15 min, centrifugal 25 min of 8 000 r/min, get supernatant liquor, add ethyl acetate solution according to volume ratio 1:1, after shaking 5 min, leave standstill 90 min, taking off a layer aqueous phase molecular weight cut-off is that after 1 kDa dialysis tubing desalination, freeze-drying obtains peeled shrimp processing fent albumen.The albumen that the manner is obtained, adds in edible film and has higher activity, has the film forming characteristics exceeding to expect.
the preparation of polypeptide in peeled shrimp processing fent:the centrifugal sediment got in step (1) adds Gly-NaOH damping fluid (0.05 mol/L, pH 9.5) according to solid-liquid ratio 1 g:3mL, adds Sumizyme MP (enzyme activity>=2.0 × 10 according to 1.0% of weight of precipitate
5u/g), in 50 DEG C of insulation 5 h, after 95 DEG C of insulation 10 min, centrifugal 20 min of 10 000 r/min, supernatant liquor adopts molecular weight cut-off to be that after 100 Da dialysis tubing desalinations, freeze-drying obtains peeled shrimp processing fent enzymolysis polypeptide.
the preparation of chitosan in peeled shrimp processing fent:the centrifugal sediment got in step (2) is washed till pH 7.0, then saturated ethylenediamine tetraacetic acid (EDTA) (EDTA) solution 24 h is added according to solid-liquid ratio 1 g:3, centrifuging and taking precipitation is washed till pH 7.0, then in precipitation, 1.0 mol/L NaOH are added, 30 h are processed at 60 DEG C, centrifuging and taking precipitation is washed till pH 7.0, dry chitosan.
the preparation of composite reactive edible film film-forming soln: chitosan prepared by albumen step (1) prepared respectively according to solid-liquid ratio 1g:50 mL and step (3) joins (wherein the ratio 1:1 of albumen and chitosan) in the lactic acid solution of 0.20 mol/L, and in solution, add 0.2% peeled shrimp processing fent enzymolysis polypeptide, 0.5% tea-polyphenol, 1.0% sorbyl alcohol according to weightmeasurement ratio, utilize defoamer to carry out deaeration after stirring, make film-forming soln.
the preparation of composite reactive edible film:be poured on equably by the film-forming soln modulated on polyvinyl chloride crystal slab and carry out striking film forming, in the 40% time drying of room temperature relative humidity after 1 day, namely demoulding obtains peeled shrimp processing fent composite reactive edible film.
Composite reactive edible film prepared by the present invention has the feature of the strong and edibility of nutritious, anti-oxidant activity, can promote the use of by food packaging applications.
Finally, still need it is noted that what enumerate above is only a specific embodiment of the present invention.Obviously, the invention is not restricted to above embodiment, many distortion can also be had.All distortion that those of ordinary skill in the art can directly derive from content disclosed by the invention or associate, all should think protection scope of the present invention.
Claims (3)
1. utilize peeled shrimp processing fent to prepare the method for composite reactive edible film, it is characterized in that: it comprises the following steps:
1) preparation of albumen in peeled shrimp processing fent:freezing shrimp meat processing fent tissue mashing machine is pulverized, the phosphate buffered saline buffer of 0.02 mol/L, pH 6.5 ~ 7.5 is added according to solid-liquid ratio 1 g:2 ~ 3mL, stir extraction 5 ~ 6 h at 35 ~ 40 DEG C after, in 85 ~ 90 DEG C of insulation 10 ~ 15 min, centrifugal 25 ~ 30 min of 8 000 r/min, get in supernatant liquor and add ethyl acetate solution according to volume ratio 1:1, after concussion 3 ~ 5 min, leave standstill 60 ~ 90 min, taking off a layer aqueous phase molecular weight cut-off is that after 1 kDa dialysis tubing desalination, freeze-drying obtains peeled shrimp processing fent albumen;
2) preparation of polypeptide in peeled shrimp processing fent:get the Gly-NaOH damping fluid that the centrifugal sediment in step 1) adds 0.05 mol/L, pH 9.0 ~ 10.0 according to solid-liquid ratio 1 g:3 ~ 5mL, add enzyme activity>=2.0 × 10 according to 0.8 ~ 1.0% of weight of precipitate
5the Sumizyme MP of U/g, in 45 ~ 55 DEG C of insulation 4 ~ 6 h, after 95 DEG C of insulation 10 min, centrifugal 15 ~ 20 min of 10 000 r/min, supernatant liquor adopts molecular weight cut-off to be that after 100 Da dialysis tubing desalinations, freeze-drying obtains peeled shrimp processing fent enzymolysis polypeptide;
3) preparation of chitosan in peeled shrimp processing fent:get step 2) in centrifugal sediment be washed till pH 6.5 ~ 7.5, then saturated edta solution 20 ~ 24 h is added according to solid-liquid ratio 1 g:3 ~ 5mL, centrifuging and taking precipitation is washed till pH 6.5 ~ 7.5, then in precipitation, 1.0 mol/L NaOH are added, 25 ~ 30 h are processed at 60 DEG C, centrifuging and taking precipitation is washed till pH 6.5 ~ 7.5, dry peeled shrimp processing fent chitosan;
4) preparation of composite reactive edible film film-forming soln: chitosan prepared by albumen step 1) prepared respectively according to solid-liquid ratio 1g:40 ~ 50 mL and step 3) shines the ratio of albumen and chitosan weight 2:1 ~ 1:2, join in the lactic acid solution of 0.20 mol/L, and 0.1 ~ 0.3% peeled shrimp processing fent enzymolysis polypeptide, 0.3 ~ 0.5% antioxidant, 0.5 ~ 1.0% softening agent is added in solution, utilize defoamer to carry out deaeration after stirring, make film-forming soln;
5) preparation of composite reactive edible film:be poured on equably by the film-forming soln modulated on polyvinyl chloride crystal slab and carry out striking film forming, in 40 ~ 50% times dryings of room temperature relative humidity after 1 ~ 2 day, namely demoulding obtains peeled shrimp processing fent composite reactive edible film.
2., according to a kind of method utilizing peeled shrimp processing fent to prepare composite reactive edible film described in claim 1, it is characterized in that: step 1) Prawn is Chinese pipe whip shrimp.
3., according to a kind of method utilizing peeled shrimp processing fent to prepare composite reactive edible film described in claim 1, it is characterized in that: step 4) in antioxidant be tea-polyphenol, softening agent is sorbyl alcohol.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110746659A (en) * | 2019-11-14 | 2020-02-04 | 广东医科大学 | Natural preservative film and preparation method thereof |
| CN115777764A (en) * | 2022-10-21 | 2023-03-14 | 自然资源部第三海洋研究所 | Edible coating liquid, composite edible film, composite coating liquid, and preparation method and application thereof |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103087355A (en) * | 2012-12-29 | 2013-05-08 | 浙江工业大学 | Tea polyphenol-containing chitosan-minced fillet protein composite film and preparation method thereof |
| CN103113496A (en) * | 2013-02-21 | 2013-05-22 | 华东理工大学 | Clean chitosan production technology |
-
2015
- 2015-05-22 CN CN201510262176.9A patent/CN104844812A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103087355A (en) * | 2012-12-29 | 2013-05-08 | 浙江工业大学 | Tea polyphenol-containing chitosan-minced fillet protein composite film and preparation method thereof |
| CN103113496A (en) * | 2013-02-21 | 2013-05-22 | 华东理工大学 | Clean chitosan production technology |
Non-Patent Citations (1)
| Title |
|---|
| MIRARI Y.ARANCIBIA ET AL.: ""Development of active films of chitosan isolated by mild extraction with added protein concentrate from shrimp waste"", 《FOOD HYDROCOLLOIDS》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110746659A (en) * | 2019-11-14 | 2020-02-04 | 广东医科大学 | Natural preservative film and preparation method thereof |
| CN115777764A (en) * | 2022-10-21 | 2023-03-14 | 自然资源部第三海洋研究所 | Edible coating liquid, composite edible film, composite coating liquid, and preparation method and application thereof |
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Application publication date: 20150819 |