CN104830826A - Preparation method and preparation apparatus of embedded immobilized microbial ball - Google Patents
Preparation method and preparation apparatus of embedded immobilized microbial ball Download PDFInfo
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- CN104830826A CN104830826A CN201510170087.1A CN201510170087A CN104830826A CN 104830826 A CN104830826 A CN 104830826A CN 201510170087 A CN201510170087 A CN 201510170087A CN 104830826 A CN104830826 A CN 104830826A
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Abstract
The present invention relates to the field of high oil-absorbing resin preparation, and discloses a preparation method of an embedded immobilized microbial ball. The method comprises the steps of: (1) preparation of the immobilized microbial particles; (2) preparation of an acrylate copolymer emulsion; and (3) preparation of the embedded immobilized microbial ball. The present invention also discloses a preparation apparatus of the embedded immobilized microbial ball. The apparatus includes an extrusion section, an insulation transport section and a moulding section. The extrusion section comprises an extrusion sections housing, an advancing screw and a rotary motor; the insulation transport section includes a tubular type conveying channel and a heater; and the moulding section comprises a moulding section housing and a pair of reverse gears provided with hemispherical grooves. The embedded immobilized microbial ball prepared by the present invention has high oil suction rate and large oil absorption capacity, and can degrade the adsorbed oil; and microbes have good stability and long survival time. At the same time, the preparation apparatus of the present invention requires mild conditions in the preparation process, protects the survival rate of the microbes to the maximum; and the prepared product has high dimensional stability.
Description
Technical field
The present invention relates to the Preparation equipment field of embedded immobilization microbial field and embedded immobilization microbial, particularly relate to preparation method and the preparation facilities thereof of embedded immobilization microbial ball.
Background technology
Come in the past few decades, the fast development of the industry of China, national life level also improves constantly, but along with industrial expansion, industry is also more and more serious to the pollution of environment.In recent years, the environmental consciousness of people is strengthened gradually, and government also more and more payes attention to for environmental improvement.
In numerous pollution source, water pollution problems is particularly outstanding, wherein, due to blowout, transport-ship be by the pollution of leaking, the leakage etc. of knocking ship and oil pipeline to sinking causes ocean, river, the serious threat to environment structure.
At present, the improvement for petroleum pollution generally adopts bioremediation technology, adopts the bacterial classification that cultivation can be degraded to petroleum hydrocarbon in water body, administers oil spilling.But in water body oil spilling microorganism remediation process, but there is effective strain and run off serious, the problem that remediation efficiency is low.Adopt immobilized microorganism to administer water body oil spilling, improve survival rate and the activity of bacterial strain to a certain extent.But the improvement of water body oil spilling, particularly marine oil spill improvement are long-term processes, and the general carrier to being fixed of microorganism is stable not, still can not ensure the long-term surviving of bacterial strain.
Also someone adopts high oil-absorbent material to carry out oil suction to water body, the swelling rate of high oil-absorbent material is high, Oil keeping is good, but after high oil-absorbent material adsorbed oil molecule reaches capacity, need to carry out Separation and Recovery, because the Oil keeping of high oil-absorbent material is good to oil and high oil-absorbent material, oil is difficult to reclaim by after high oil-absorbent material absorption, current separation and recovery technology energy consumption is high, and complicated operation, cost is high.
Summary of the invention
Short in order to solve the survival time of immobilized microorganism in prior art, and to the separating of oil technical problem reclaiming difficulty after high oil-absorbent material oil suction, the invention provides a kind of preparation method of embedded immobilization microbial ball, embedded immobilization microbial ball swelling rate prepared by the method is fast, oil number is large, and microbiological deterioration can be carried out to by the oil after adsorbing, and microorganism is at the good stability of carrier, survival time is in the carrier long, makes it possible to the oil spilling removed long-term effectively in water body.The invention provides a kind of preparation facilities of immobilized microorganism ball, this device preparation condition in preparation immobilized microorganism ball process is gentle, farthest ensured the survival rate of microorganism in preparation process, and the Product size stability of preparation is high simultaneously.
Concrete technical scheme of the present invention is: a kind of preparation method of embedded immobilization microbial ball, and concrete steps are as follows:
(1) preparation of immobilization microorganism particles:
Get 10 parts of modification halloysite nanotubes and 40-80 part petroleum hydrocarbon degradation bacteria concentration is 6 × 10
9the kind daughter bacteria liquid mixing of cell/g, until described modification halloysite nanotubes adsorbs after described kind of daughter bacteria liquid reaches capacity, obtains the modification halloysite nanotubes liquid of attracts bacteria;
In the modification halloysite nanotubes liquid of above-mentioned attracts bacteria, add 1000 parts of concentration is the sodium alginate soln of 4-8wt%, mixing solutions is obtained after mixing, then above-mentioned mixing solutions being injected into concentration is in the calcium chloride solution of 2.5wt-3.5wt%, leaches obtained immobilization microorganism particles after placing 2-4h.
When mixing solutions is injected into calcium chloride solution, after calcium chloride and sodium alginate form alginate calcium, form carrier with halloysite nanotubes.
(2) preparation of acrylate copolymer emulsion:
Get 65-85 part methacrylate-based monomer, 20-30 part vinylbenzene, 0.5-1.5 part initiator, 3-7 part emulsifying agent join stirring and emulsifying 1-2h in 250-450 part water, obtain emulsion.
Add 3-5 part linking agent, 45-55 part perforating agent in above-mentioned emulsion after, logical nitrogen carries out heated polymerizable reaction, and temperature of reaction is 65-85 DEG C, and the reaction times is 4-8h, obtains acrylate copolymer emulsion after reaction terminates.
(3) preparation of embedded immobilization microbial ball:
Get the above-mentioned immobilization microorganism particles of 5-20 part, 0.1-0.5 part coupling agent joins in aforesaid propylene acid ester copolymer emulsion, at normal temperatures stirring at low speed evenly after obtain mixture, described mixture is put into embedded immobilization microbial ball preparation facilities and carry out ball forming processed, by the product after shaping after physiological saline cleaning, then after vacuum-drying obtained embedded immobilization microbial ball.
Each component score is parts by weight above.
In above-mentioned embedded immobilization microbial ball, due to the existence of perforating agent, make embedded immobilization microbial ball inside have a large amount of fine pore passage structures, thus there is fast absorbing oil ability and larger oil tankage; And immobilized microorganism is first carried in halloysite nanotubes-sodium alginate-Calcium Chloride System, is being carried in resin further, there is stronger stability.
The present invention simultaneously first makes the first copolymerization such as methacrylate-based monomer and vinylbenzene to a certain degree, compound together with immobilization microorganism particles etc. again, due to be under normal temperature stirring at low speed condition with acrylate copolymer emulsion compound, avoid high temperature high stir speed (S.S.), polymeric inner network structure formation condition is comparatively gentle, farthest guarantees that microorganism cells is without prejudice.
Further, described in step (1), the preparation method of modification halloysite nanotubes is:
Got 60-80 order halloysite nanotubes to join concentration be in the citric acid solution of 3wt% and stirred, and obtaining acid activation halloysite nanotubes liquid after acid activation 2-4h, the weight consumption of described halloysite nanotubes and citric acid is than being 1:2-4;
Above-mentioned acid activation halloysite nanotubes liquid is carried out thermal activation at 400-600 DEG C, takes out after 1-2h and obtain thermal activation halloysite nanotubes;
Modification halloysite nanotubes was obtained after above-mentioned thermal activation halloysite nanotubes is pulverized 150-200 order.
Its adsorptive power modified carried out to halloysite nanotubes stronger.
Further, methacrylate-based monomer described in step (2) is selected from least one in lauryl methacrylate, methacrylic acid 13 ester, and methyl methacrylate, β-dimethyl-aminoethylmethacrylate, at least one in butyl methacrylate.
Further, emulsifying agent described in step (2) is Sodium dodecylbenzene sulfonate, described initiator is persulphate, described linking agent is N, N '-methylene-bisacrylamide, described perforating agent are ethyl acetate, and described in step (3), coupling agent is silane coupling agent.
Further, together join the nano titanium oxide in addition in described acrylate copolymer emulsion with described immobilization microorganism particles, coupling agent in step (3), the consumption of described part nano titanium oxide is 1-5 part, and the particle diameter of nano titanium oxide is 10-50 nanometer.
Adding of nano titanium oxide; embedded immobilization microbial ball is made to have stronger resistance of aging; particularly anti-uv-ray; because for process rivers; particularly during deepwater oil slick; usually can suffer long Exposure to Sunlight, improve the practical life that anti-light aging ability contributes to extending resin.
Further, the petroleum hydrocarbon degradation bacterium described in step (1) is bacillus brevis D-1.
A kind of preparation facilities preparing above-mentioned embedded immobilization microbial ball, comprise connect successively extruding zone, insulation transportation section and profiled section, described extruding zone comprises extruding zone housing, augering screw and rotating machine, described augering screw is located on the axis of housing, one end of described augering screw is extended housing and is connected with rotating machine, and described extruding zone housing is provided with opening for feed; Described insulation transportation section comprises tubular type feed track and is located at the well heater of tubular type feed track outer wall, and described tubular type feed track is coaxial with extruding zone housing; The reverse gear that described profiled section comprises profiled section housing and is fixed on for a pair in profiled section housing, described reverse gear is provided with dome-type groove, described reverse gear is rotated by the electric machine control be located at outside profiled section housing, and described profiled section housing is provided with discharge port.
After material is added from opening for feed, rotating machine controls augering screw and rotates, material is advanced insulation transportation section, well heater carries out the insulation of 30-50 DEG C to material, holding temperature is lower, can not produce excessive impact to the survival of microorganism, after material arrives profiled section, by the continuous pelletizing of dome-type groove on a pair reverse gear, formed spherical after the dome-type groove on two reverse gears merges.
Further, the internal diameter of described tubular type feed track is 2-6 millimeter, and the diameter of first ball-type groove of described reverse gear is 3-8 millimeter.
Further, the points of tangency of described two reverse gears is positioned at extruding zone, is incubated on the axis of transportation section and profiled section.
Further, be positioned at the gear sense of rotation of top in described reverse gear for counterclockwise, the gear sense of rotation being positioned at below is clockwise direction.
Be compared with the prior art, the invention has the beneficial effects as follows: the fine pore passage structure of embedded immobilization microbial ball inside prepared by the present invention is numerous, there is swelling rate, larger absorbency capacity and outstanding Oil keeping fast.Inside is compounded with immobilized microorganism simultaneously, the oil be absorbed into can be decomposed, immobilized microorganism is the first carrier with modification halloysite nanotubes-sodium alginate-calcium chloride, take compound resin as Second support, to the good stability of microorganism solidification, and modification halloysite nanotubes has vesicular structure, also there is good adsorptivity, nutritive substance can be made more easily by microorganism panning, and improve survival rate and the activity of microorganism, degradation efficiency is high.This embedded immobilization microbial ball by the oily disintegrate of absorption, can carry out oil suction and oil degraded, without the need to carrying out Separation and Recovery simultaneously.
The preparation facilities of immobilized microorganism ball of the present invention simultaneously, this device preparation condition in preparation immobilized microorganism ball process is gentle, farthest ensured the survival rate of microorganism in preparation process, and the Product size stability of preparation is high.
Accompanying drawing explanation
Fig. 1 is the preparation facilities of a kind of embedded immobilization microbial ball in the present invention.
Reference numeral is: extruding zone housing 1, augering screw 2, rotating machine 3, opening for feed 4, tubular type feed track 5, well heater 6, profiled section housing 7, reverse gear 8, dome-type groove 9, discharge port 10.
Embodiment
Below in conjunction with embodiment, the invention will be further described.
As shown in Figure 1: a kind of preparation facilities preparing embedded immobilization microbial ball, comprise connect successively extruding zone, insulation transportation section and profiled section.
Described extruding zone comprises extruding zone housing 1, augering screw 2 and rotating machine 3, described augering screw is located on the axis of extruding zone housing, one end of described augering screw is extended extruding zone housing and is connected with rotating machine, and described extruding zone housing is provided with opening for feed 4.
Described insulation transportation section comprises tubular type feed track 5 and is located at the well heater 6 of tubular type feed track outer wall, and described tubular type feed track is coaxial and the internal diameter of tubular type feed track is 2-6 millimeter with extruding zone housing.
Described profiled section comprises profiled section housing 7 and is fixed on the reverse gear 8 in profiled section housing for a pair, and the points of tangency of described two reverse gears is positioned at extruding zone, is incubated on the axis of transportation section and profiled section.Described reverse gear is provided with dome-type groove 9, the diameter 1-2 millimeter larger than the internal diameter of tubular type feed track of described dome-type groove, described reverse gear is rotated by the electric machine control be located at outside profiled section housing, be positioned at the gear sense of rotation of top in described reverse gear for counterclockwise, the gear sense of rotation being positioned at below is clockwise direction.Described profiled section housing is provided with discharge port 10.
Embodiment 1
A preparation method for embedded immobilization microbial ball, concrete steps are as follows:
(1) preparation of immobilization microorganism particles:
Got 80 order halloysite nanotubes to join concentration be in the citric acid solution of 3wt% and stirred, and obtaining acid activation halloysite nanotubes liquid after acid activation 3h, the weight consumption of described halloysite nanotubes and citric acid is than being 1:3.
Above-mentioned acid activation halloysite nanotubes liquid is carried out thermal activation at 500 DEG C, takes out after 1.5h and obtain thermal activation halloysite nanotubes.Modification halloysite nanotubes was obtained after thermal activation halloysite nanotubes is pulverized 200 orders.
Get 10 parts of modification halloysite nanotubes and 60 parts of bacillus brevis D-1 concentration are 6 × 10
9the kind daughter bacteria liquid mixing of cell/g, until described modification halloysite nanotubes adsorbs after described kind of daughter bacteria liquid reaches capacity, obtains the modification halloysite nanotubes liquid of attracts bacteria.
In the modification halloysite nanotubes liquid of above-mentioned attracts bacteria, add 1000 parts of concentration is the sodium alginate soln of 6wt%, mixing solutions is obtained after mixing, then above-mentioned mixing solutions being injected into concentration is in the calcium chloride solution of 3wt%, leaches obtained immobilization microorganism particles after placing 3h.
(2) preparation of acrylate copolymer emulsion:
Get 50 parts of lauryl methacrylate, 25 parts of methyl methacrylates, 25 parts of vinylbenzene, 1 part of Potassium Persulphate, 5 parts of Sodium dodecylbenzene sulfonatees join stirring and emulsifying 1.5h in 350 parts of water, obtain emulsion;
In above-mentioned emulsion, after interpolation 4 parts of N, N '-methylene-bisacrylamide, 50 parts of ethyl acetate, logical nitrogen carries out heated polymerizable reaction, and temperature of reaction is 75 DEG C, and the reaction times is 6h, obtains acrylate copolymer emulsion after reaction terminates;
(3) preparation of embedded immobilization microbial ball:
Get 12 parts of above-mentioned immobilization microorganism particles, 3 parts of silane coupling agents join in aforesaid propylene acid ester copolymer emulsion, at normal temperatures stirring at low speed evenly after obtain mixture, described mixture is put into embedded immobilization microbial ball preparation facilities and carry out ball forming processed, by the product after shaping after physiological saline cleaning, then after vacuum-drying obtained embedded immobilization microbial ball;
Each component score is parts by weight above.
Embodiment 2
A preparation method for embedded immobilization microbial ball, concrete steps are as follows:
(1) preparation of immobilization microorganism particles:
Got 60 order halloysite nanotubes to join concentration be in the citric acid solution of 3wt% and stirred, and obtaining acid activation halloysite nanotubes liquid after acid activation 2h, the weight consumption of described halloysite nanotubes and citric acid is than being 1:4.
Above-mentioned acid activation halloysite nanotubes liquid is carried out thermal activation at 400 DEG C, takes out after 2h and obtain thermal activation halloysite nanotubes.Modification halloysite nanotubes was obtained after thermal activation halloysite nanotubes is pulverized 150 orders.
Get 10 parts of modification halloysite nanotubes and 40 parts of bacillus brevis D-1 concentration are 6 × 10
9the kind daughter bacteria liquid mixing of cell/g, until described modification halloysite nanotubes adsorbs after described kind of daughter bacteria liquid reaches capacity, obtains the modification halloysite nanotubes liquid of attracts bacteria.
In the modification halloysite nanotubes liquid of above-mentioned attracts bacteria, add 1000 parts of concentration is the sodium alginate soln of 4wt%, mixing solutions is obtained after mixing, then above-mentioned mixing solutions being injected into concentration is in the calcium chloride solution of 2.5wtwt%, leaches obtained immobilization microorganism particles after placing 4h.
(2) preparation of acrylate copolymer emulsion:
Get 40 parts of methacrylic acid 13 esters, 30 parts of butyl methacrylate, 20 parts of vinylbenzene, 1.5 parts of ammonium persulphates, 3 parts of Sodium dodecylbenzene sulfonatees join stirring and emulsifying 2h in 450 parts of water, obtain emulsion;
In above-mentioned emulsion, after interpolation 3 parts of N, N '-methylene-bisacrylamide, 45 parts of ethyl acetate, logical nitrogen carries out heated polymerizable reaction, and temperature of reaction is 65 DEG C, and the reaction times is 8h, obtains acrylate copolymer emulsion after reaction terminates;
(3) preparation of embedded immobilization microbial ball:
Get 15 parts of above-mentioned immobilization microorganism particles, nano titanium oxide that 0.2 part of silane coupling agent, 5 parts of particle diameters are 20 ran joins in aforesaid propylene acid ester copolymer emulsion, at normal temperatures stirring at low speed evenly after obtain mixture, described mixture is put into embedded immobilization microbial ball preparation facilities and carry out ball forming processed, by the product after shaping after physiological saline cleaning, then after vacuum-drying obtained embedded immobilization microbial ball;
Each component score is parts by weight above.
Embodiment 3
A preparation method for embedded immobilization microbial ball, concrete steps are as follows:
(1) preparation of immobilization microorganism particles:
Got 60 order halloysite nanotubes to join concentration be in the citric acid solution of 3wt% and stirred, and obtaining acid activation halloysite nanotubes liquid after acid activation 1h, the weight consumption of described halloysite nanotubes and citric acid is than being 1:2.
Above-mentioned acid activation halloysite nanotubes liquid is carried out thermal activation at 400 DEG C, takes out after 1h and obtain thermal activation halloysite nanotubes.Modification halloysite nanotubes was obtained after thermal activation halloysite nanotubes is pulverized 200 orders.
Get 10 parts of modification halloysite nanotubes and 30 parts of bacillus brevis D-1 concentration are 6 × 10
9the kind daughter bacteria liquid mixing of cell/g, until described modification halloysite nanotubes adsorbs after described kind of daughter bacteria liquid reaches capacity, obtains the modification halloysite nanotubes liquid of attracts bacteria.
In the modification halloysite nanotubes liquid of above-mentioned attracts bacteria, add 1000 parts of concentration is the sodium alginate soln of 6wt%, mixing solutions is obtained after mixing, then above-mentioned mixing solutions being injected into concentration is in the calcium chloride solution of 3wtwt%, leaches obtained immobilization microorganism particles after placing 5h.
(2) preparation of acrylate copolymer emulsion:
Get 15 parts of methacrylic acid 13 esters, 15 parts of lauryl methacrylate, 20 parts of butyl methacrylate, 25 parts of vinylbenzene, 0.5 part of ammonium persulphate, 3 parts of Sodium dodecylbenzene sulfonatees join stirring and emulsifying 2h in 350 parts of water, obtain emulsion;
In above-mentioned emulsion, after interpolation 7 parts of N, N '-methylene-bisacrylamide, 65 parts of ethyl acetate, logical nitrogen carries out heated polymerizable reaction, and temperature of reaction is 80 DEG C, and the reaction times is 16h, obtains acrylate copolymer emulsion after reaction terminates;
(3) preparation of embedded immobilization microbial ball:
Get 25 parts of above-mentioned immobilization microorganism particles, nano titanium oxide that 0.5 part of silane coupling agent, 5 parts of particle diameters are 40 ran joins in aforesaid propylene acid ester copolymer emulsion, at normal temperatures stirring at low speed evenly after obtain mixture, described mixture is put into embedded immobilization microbial ball preparation facilities and carry out ball forming processed, by the product after shaping after physiological saline cleaning, then after vacuum-drying obtained embedded immobilization microbial ball;
Each component score is parts by weight above.
Embodiment 4
A preparation method for embedded immobilization microbial ball, concrete steps are as follows:
(1) preparation of immobilization microorganism particles:
Got 60 order halloysite nanotubes to join concentration be in the citric acid solution of 3wt% and stirred, and obtaining acid activation halloysite nanotubes liquid after acid activation 1h, the weight consumption of described halloysite nanotubes and citric acid is than being 1:2.5.
Above-mentioned acid activation halloysite nanotubes liquid is carried out thermal activation at 450 DEG C, takes out after 1.5h and obtain thermal activation halloysite nanotubes.Modification halloysite nanotubes was obtained after thermal activation halloysite nanotubes is pulverized 150 orders.
Get 10 parts of modification halloysite nanotubes and 40 parts of bacillus brevis D-1 concentration are 6 × 10
9the kind daughter bacteria liquid mixing of cell/g, until described modification halloysite nanotubes adsorbs after described kind of daughter bacteria liquid reaches capacity, obtains the modification halloysite nanotubes liquid of attracts bacteria.
In the modification halloysite nanotubes liquid of above-mentioned attracts bacteria, add 1000 parts of concentration is the sodium alginate soln of 7wt%, mixing solutions is obtained after mixing, then above-mentioned mixing solutions being injected into concentration is in the calcium chloride solution of 3wtwt%, leaches obtained immobilization microorganism particles after placing 5h.
(2) preparation of acrylate copolymer emulsion:
Get 15 parts of methacrylic acid 13 esters, 15 parts of lauryl methacrylate, 25 parts of methyl methacrylates, 25 parts of vinylbenzene, 1.5 parts of ammonium persulphates, 3 parts of Sodium dodecylbenzene sulfonatees join stirring and emulsifying 2h in 350 parts of water, obtain emulsion;
In above-mentioned emulsion, after interpolation 3 parts of N, N '-methylene-bisacrylamide, 45 parts of ethyl acetate, logical nitrogen carries out heated polymerizable reaction, and temperature of reaction is 75 DEG C, and the reaction times is 10h, obtains acrylate copolymer emulsion after reaction terminates;
(3) preparation of embedded immobilization microbial ball:
Get 20 parts of above-mentioned immobilization microorganism particles, nano titanium oxide that 0.4 part of silane coupling agent, 3 parts of particle diameters are 30 ran joins in aforesaid propylene acid ester copolymer emulsion, at normal temperatures stirring at low speed evenly after obtain mixture, described mixture is put into embedded immobilization microbial ball preparation facilities and carry out ball forming processed, by the product after shaping after physiological saline cleaning, then after vacuum-drying obtained embedded immobilization microbial ball;
Each component score is parts by weight above.
The above; it is only preferred embodiment of the present invention; not the present invention is imposed any restrictions, every above embodiment is done according to the technology of the present invention essence any simple modification, change and equivalent structure transformation, all still belong to the protection domain of technical solution of the present invention.
Claims (10)
1. a preparation method for embedded immobilization microbial ball, is characterized in that concrete steps are as follows:
(1) preparation of immobilization microorganism particles:
Get 10 parts of modification halloysite nanotubes and 40-80 part petroleum hydrocarbon degradation bacteria concentration is 6 × 10
9the kind daughter bacteria liquid mixing of cell/g, until described modification halloysite nanotubes adsorbs after described kind of daughter bacteria liquid reaches capacity, obtains the modification halloysite nanotubes liquid of attracts bacteria;
In the modification halloysite nanotubes liquid of above-mentioned attracts bacteria, add 1000 parts of concentration is the sodium alginate soln of 4-8wt%, mixing solutions is obtained after mixing, then above-mentioned mixing solutions being injected into concentration is in the calcium chloride solution of 2.5wt-3.5wt%, leaches obtained immobilization microorganism particles after placing 2-4h;
(2) preparation of acrylate copolymer emulsion:
Get 65-85 part methacrylate-based monomer, 20-30 part vinylbenzene, 0.5-1.5 part initiator, 3-7 part emulsifying agent join stirring and emulsifying 1-2h in 250-450 part water, obtain emulsion;
Add 3-5 part linking agent, 45-55 part perforating agent in above-mentioned emulsion after, logical nitrogen carries out heated polymerizable reaction, and temperature of reaction is 65-85 DEG C, and the reaction times is 4-8h, obtains acrylate copolymer emulsion after reaction terminates;
(3) preparation of embedded immobilization microbial ball:
Get the above-mentioned immobilization microorganism particles of 5-20 part, 0.1-0.5 part coupling agent joins in aforesaid propylene acid ester copolymer emulsion, at normal temperatures stirring at low speed evenly after obtain mixture, described mixture is put into embedded immobilization microbial ball preparation facilities and carry out ball forming processed, by the product after shaping after physiological saline cleaning, then after vacuum-drying obtained embedded immobilization microbial ball;
Each component score is parts by weight above.
2. the preparation method of embedded immobilization microbial ball according to claim 1, is characterized in that, described in step (1), the preparation method of modification halloysite nanotubes is:
Got 60-80 order halloysite nanotubes to join concentration be in the citric acid solution of 3wt% and stirred, and obtaining acid activation halloysite nanotubes liquid after acid activation 2-4h, the weight consumption of described halloysite nanotubes and citric acid is than being 1:2-4;
Above-mentioned acid activation halloysite nanotubes liquid is carried out thermal activation at 400-600 DEG C, takes out after 1-2h and obtain thermal activation halloysite nanotubes;
Modification halloysite nanotubes was obtained after above-mentioned thermal activation halloysite nanotubes is pulverized 150-200 order.
3. the preparation method of embedded immobilization microbial ball according to claim 1, it is characterized in that, methacrylate-based monomer described in step (2) is selected from least one in lauryl methacrylate, methacrylic acid 13 ester, and methyl methacrylate, β-dimethyl-aminoethylmethacrylate, at least one in butyl methacrylate.
4. the preparation method of embedded immobilization microbial ball according to claim 1, it is characterized in that, emulsifying agent described in step (2) is Sodium dodecylbenzene sulfonate, described initiator is persulphate, described linking agent is N, N '-methylene-bisacrylamide, described perforating agent are ethyl acetate, and described in step (3), coupling agent is silane coupling agent.
5. the preparation method of embedded immobilization microbial ball according to claim 1, it is characterized in that, together join the nano titanium oxide in addition in described acrylate copolymer emulsion with described immobilization microorganism particles, coupling agent in step (3), the consumption of described part nano titanium oxide is 1-5 part, and the particle diameter of nano titanium oxide is 10-50 nanometer.
6. the preparation method of embedded immobilization microbial ball according to claim 1, is characterized in that, the petroleum hydrocarbon degradation bacterium described in step (1) is bacillus brevis D-1.
7. prepare the preparation facilities of the embedded immobilization microbial ball as described in one of claim 1-6 for one kind, it is characterized in that: comprise connect successively extruding zone, insulation transportation section and profiled section, described extruding zone comprises extruding zone housing (1), augering screw (2) and rotating machine (3), described augering screw is located on the axis of extruding zone housing, one end of described augering screw is extended extruding zone housing and is connected with rotating machine, and described extruding zone housing is provided with opening for feed (4); Described insulation transportation section comprises tubular type feed track (5) and is located at the well heater (6) of tubular type feed track outer wall, and described tubular type feed track is coaxial with extruding zone housing; Described profiled section comprises profiled section housing (7) and is fixed on the reverse gear (8) in profiled section housing for a pair, described reverse gear is provided with dome-type groove (9), described reverse gear is rotated by the electric machine control be located at outside profiled section housing, and described profiled section housing is provided with discharge port (10).
8. the preparation facilities of embedded immobilization microbial ball according to claim 7, is characterized in that, the internal diameter of described tubular type feed track is 2-6 millimeter, and the diameter of first ball-type groove of described reverse gear is 3-8 millimeter.
9. the preparation facilities of embedded immobilization microbial ball according to claim 7, is characterized in that, the points of tangency of described two reverse gears is positioned at extruding zone, is incubated on the axis of transportation section and profiled section.
10. the preparation facilities of embedded immobilization microbial ball according to claim 7, is characterized in that, be positioned at the gear sense of rotation of top in described reverse gear for counterclockwise, the gear sense of rotation being positioned at below is clockwise direction.
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| CN107837795A (en) * | 2017-11-17 | 2018-03-27 | 浙江海洋大学 | A kind of resin sorbent and preparation method of the load of microorganisms type of degradable greasy dirt |
| CN108117971A (en) * | 2016-11-30 | 2018-06-05 | 斗山生物技术有限公司 | Utilize the microbial fixed carrier preparation facilities for transferring bolt |
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| CN109046290A (en) * | 2018-09-12 | 2018-12-21 | 潘钕 | The preparation and application of fly ash base resin sorbent |
| CN109706072A (en) * | 2019-01-24 | 2019-05-03 | 王静 | A kind of microbe immobilized particles preparation facilities |
| CN110773545A (en) * | 2019-09-19 | 2020-02-11 | 百沃星联(上海)环保科技有限公司 | Microorganism medium for food decomposition, and apparatus and method for producing the same |
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| CN110773545A (en) * | 2019-09-19 | 2020-02-11 | 百沃星联(上海)环保科技有限公司 | Microorganism medium for food decomposition, and apparatus and method for producing the same |
| CN113620653A (en) * | 2021-09-03 | 2021-11-09 | 科顺民用建材有限公司 | Microbial emulsion, composition thereof, preparation method and application thereof, and microbial self-repairing high-temperature-resistant tile glue |
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