CN104820047A - 采用sle法同时分离猪尿中的莱克多巴胺、克仑特罗、沙丁胺醇的方法 - Google Patents
采用sle法同时分离猪尿中的莱克多巴胺、克仑特罗、沙丁胺醇的方法 Download PDFInfo
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- CN104820047A CN104820047A CN201510238888.7A CN201510238888A CN104820047A CN 104820047 A CN104820047 A CN 104820047A CN 201510238888 A CN201510238888 A CN 201510238888A CN 104820047 A CN104820047 A CN 104820047A
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- Prior art keywords
- sle
- ractopamine
- clenbuterol
- salbutamol
- pig urine
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- 238000000034 method Methods 0.000 title claims abstract description 46
- 229940074095 ractopamine Drugs 0.000 title claims abstract description 35
- YJQZYXCXBBCEAQ-UHFFFAOYSA-N ractopamine Chemical compound C=1C=C(O)C=CC=1C(O)CNC(C)CCC1=CC=C(O)C=C1 YJQZYXCXBBCEAQ-UHFFFAOYSA-N 0.000 title claims abstract description 35
- NDAUXUAQIAJITI-UHFFFAOYSA-N albuterol Chemical compound CC(C)(C)NCC(O)C1=CC=C(O)C(CO)=C1 NDAUXUAQIAJITI-UHFFFAOYSA-N 0.000 title claims abstract description 33
- 229960001117 clenbuterol Drugs 0.000 title claims abstract description 33
- STJMRWALKKWQGH-UHFFFAOYSA-N clenbuterol Chemical compound CC(C)(C)NCC(O)C1=CC(Cl)=C(N)C(Cl)=C1 STJMRWALKKWQGH-UHFFFAOYSA-N 0.000 title claims abstract description 33
- 229960002052 salbutamol Drugs 0.000 title claims abstract description 33
- 238000000605 extraction Methods 0.000 title abstract description 6
- 239000007788 liquid Substances 0.000 title abstract description 5
- 210000002700 urine Anatomy 0.000 claims abstract description 42
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 claims abstract description 9
- 102000009133 Arylsulfatases Human genes 0.000 claims abstract description 7
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 claims abstract description 7
- 108060007951 sulfatase Proteins 0.000 claims abstract description 7
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 6
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 6
- USFZMSVCRYTOJT-UHFFFAOYSA-N Ammonium acetate Chemical compound N.CC(O)=O USFZMSVCRYTOJT-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000005695 Ammonium acetate Substances 0.000 claims abstract description 5
- 229940043376 ammonium acetate Drugs 0.000 claims abstract description 5
- 235000019257 ammonium acetate Nutrition 0.000 claims abstract description 5
- 238000002156 mixing Methods 0.000 claims abstract description 5
- 239000007853 buffer solution Substances 0.000 claims abstract description 4
- WYURNTSHIVDZCO-UHFFFAOYSA-N Tetrahydrofuran Chemical compound C1CCOC1 WYURNTSHIVDZCO-UHFFFAOYSA-N 0.000 claims description 24
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 20
- 239000003480 eluent Substances 0.000 claims description 20
- YLQBMQCUIZJEEH-UHFFFAOYSA-N tetrahydrofuran Natural products C=1C=COC=1 YLQBMQCUIZJEEH-UHFFFAOYSA-N 0.000 claims description 12
- 239000006228 supernatant Substances 0.000 claims description 10
- -1 alditol glycosides Chemical class 0.000 claims description 8
- 102000004190 Enzymes Human genes 0.000 claims description 6
- 108090000790 Enzymes Proteins 0.000 claims description 6
- 241000219095 Vitis Species 0.000 claims description 6
- 235000009754 Vitis X bourquina Nutrition 0.000 claims description 6
- 235000012333 Vitis X labruscana Nutrition 0.000 claims description 6
- 235000014787 Vitis vinifera Nutrition 0.000 claims description 6
- 229930182470 glycoside Natural products 0.000 claims description 6
- 238000000622 liquid--liquid extraction Methods 0.000 claims description 6
- 229920001223 polyethylene glycol Polymers 0.000 claims description 6
- 239000000243 solution Substances 0.000 claims description 6
- 238000000638 solvent extraction Methods 0.000 claims description 6
- 238000003916 acid precipitation Methods 0.000 claims description 4
- 239000000872 buffer Substances 0.000 claims description 4
- 238000012545 processing Methods 0.000 claims description 4
- 238000005119 centrifugation Methods 0.000 claims description 2
- 102000053187 Glucuronidase Human genes 0.000 abstract 1
- 108010060309 Glucuronidase Proteins 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 abstract 1
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 6
- 238000001514 detection method Methods 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 239000002904 solvent Substances 0.000 description 4
- WEVYAHXRMPXWCK-UHFFFAOYSA-N Acetonitrile Chemical compound CC#N WEVYAHXRMPXWCK-UHFFFAOYSA-N 0.000 description 3
- 238000004090 dissolution Methods 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000008346 aqueous phase Substances 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 239000012071 phase Substances 0.000 description 2
- 239000004743 Polypropylene Substances 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 239000012491 analyte Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 102000012740 beta Adrenergic Receptors Human genes 0.000 description 1
- 108010079452 beta Adrenergic Receptors Proteins 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000002596 correlated effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
- 239000002024 ethyl acetate extract Substances 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 238000010813 internal standard method Methods 0.000 description 1
- 125000001449 isopropyl group Chemical group [H]C([H])([H])C([H])(*)C([H])([H])[H] 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 229920001155 polypropylene Polymers 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 238000005464 sample preparation method Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
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Priority Applications (1)
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CN201510238888.7A CN104820047B (zh) | 2015-05-12 | 2015-05-12 | 采用sle法同时分离猪尿中的莱克多巴胺、克仑特罗、沙丁胺醇的方法 |
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CN201510238888.7A CN104820047B (zh) | 2015-05-12 | 2015-05-12 | 采用sle法同时分离猪尿中的莱克多巴胺、克仑特罗、沙丁胺醇的方法 |
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CN104820047A true CN104820047A (zh) | 2015-08-05 |
CN104820047B CN104820047B (zh) | 2016-06-29 |
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CN201510238888.7A Active CN104820047B (zh) | 2015-05-12 | 2015-05-12 | 采用sle法同时分离猪尿中的莱克多巴胺、克仑特罗、沙丁胺醇的方法 |
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Citations (5)
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---|---|---|---|---|
JPH0767692A (ja) * | 1993-09-06 | 1995-03-14 | Shinwa Kako Kk | 光学異性体用分離剤 |
WO2005089741A2 (en) * | 2004-03-17 | 2005-09-29 | Sosei R&D Ltd. | The treatment of inflammatory disorders and pain using beta-aminoalcohols |
CN101023171A (zh) * | 2004-08-02 | 2007-08-22 | 根特拉体系股份有限公司 | 采用固相支持物纯化dna的制剂和方法 |
CN103353488A (zh) * | 2013-06-19 | 2013-10-16 | 中华人民共和国潍坊出入境检验检疫局 | 一种测定蔬果中百菌清、克菌丹、敌菌丹和灭菌丹残留的方法 |
CN103443621A (zh) * | 2011-02-07 | 2013-12-11 | 美国控股实验室公司 | 用于测定睾酮在样品中的存在或量的方法和*** |
-
2015
- 2015-05-12 CN CN201510238888.7A patent/CN104820047B/zh active Active
Patent Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPH0767692A (ja) * | 1993-09-06 | 1995-03-14 | Shinwa Kako Kk | 光学異性体用分離剤 |
WO2005089741A2 (en) * | 2004-03-17 | 2005-09-29 | Sosei R&D Ltd. | The treatment of inflammatory disorders and pain using beta-aminoalcohols |
CN101023171A (zh) * | 2004-08-02 | 2007-08-22 | 根特拉体系股份有限公司 | 采用固相支持物纯化dna的制剂和方法 |
CN103443621A (zh) * | 2011-02-07 | 2013-12-11 | 美国控股实验室公司 | 用于测定睾酮在样品中的存在或量的方法和*** |
CN103353488A (zh) * | 2013-06-19 | 2013-10-16 | 中华人民共和国潍坊出入境检验检疫局 | 一种测定蔬果中百菌清、克菌丹、敌菌丹和灭菌丹残留的方法 |
Non-Patent Citations (9)
Title |
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A. KAUFMANN 等: "Multi-residue quantification of veterinary drugs in milk with a novel extraction and cleanup technique: Salting out supported liquid extraction (SOSLE)", 《ANALYTICA CHIMICA ACTA》, vol. 820, 26 February 2014 (2014-02-26), pages 56 - 68 * |
E.N. SAUVE 等: "Determination of benzodiazepines in ante-mortem and post-mortem whole blood by solid-supported liquid–liquid extraction and UPLC–MS/MS", 《JOURNAL OF CHROMATOGRAPHY B》, vol. 883884, 1 February 2012 (2012-02-01), pages 177 - 188 * |
JUAN C. DOMÍNGUEZ-ROMERO 等: "Comparative evaluation of seven different sample treatment approaches for large-scale multiclass sport drug testing in urine by liquid chromatography–mass spectrometry", 《JOURNAL OF CHROMATOGRAPHY A》, vol. 1361, 26 September 2014 (2014-09-26), pages 34 - 42, XP055182083, DOI: doi:10.1016/j.chroma.2014.07.090 * |
P. M. WYNNE 等: "Approaches to the Solid-Phase Extraction of Equine Urine", 《CHROMATOGRAPHIA》, vol. 59, 31 March 2004 (2004-03-31), pages 51 - 56 * |
包建民 等: "一种以含硅藻土的复合材料为介质的支撑液液萃取柱的制备及其应用", 《色谱》, vol. 30, no. 8, 31 August 2012 (2012-08-31), pages 798 - 803 * |
曾绍东 等: "超高效液相色谱-质谱法测定猪肉中9种β-受体激动剂残留", 《分析仪器》, no. 4, 31 August 2013 (2013-08-31), pages 5 - 11 * |
朱鹏飞 等: "UHPLC-QTrap同时检测动物源性样品中七种β2-受体激动剂", 《第六届华东地区色谱质谱学术报告会论文集》, 7 November 2014 (2014-11-07), pages 1 - 9 * |
王丽 等: "固相支持液液萃取- 气相色谱法测定淡水鱼及水体中共平面多氯联苯", 《现代预防医学》, vol. 39, no. 23, 31 December 2012 (2012-12-31), pages 6238 - 6241 * |
王立琦 等: "液相色谱-电喷雾串联质谱测定猪组织中β-***残留的基质效应", 《分析化学》, vol. 40, no. 9, 30 September 2012 (2012-09-30), pages 1445 - 1449 * |
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Effective date of registration: 20170122 Address after: 543000 the Guangxi Zhuang Autonomous Region Cenxi North Road No. 5 Patentee after: Cenxi Productivity Promotion Center Address before: 543000 the Guangxi Zhuang Autonomous Region, Wuzhou West Ring Road, No. 198 Patentee before: WUZHOU INSTITUTE FOR FOOD AND DRUG CONTROL, GUANGXI ZHUANG AUTONOMOUS REGION |
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CB03 | Change of inventor or designer information | ||
CB03 | Change of inventor or designer information |
Inventor after: Sun Liangguang Inventor after: Guo Zhenwang Inventor after: Chen Xuesong Inventor after: Chen Qizhao Inventor after: Ye Xiaoqiang Inventor after: Wu Lan Inventor after: Deng Shuide Inventor before: Chen Xuesong Inventor before: Chen Qizhao Inventor before: Ye Xiaoqiang Inventor before: Guo Zhenwang Inventor before: Wu Lan Inventor before: Deng Shuide |
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Effective date of registration: 20180426 Address after: 543000 198 West Ring Road, Wuzhou, the Guangxi Zhuang Autonomous Region Patentee after: WUZHOU FOOD AND DRUG INSPECTION INSTITUTE Address before: No. 5 north mountain road, Cenxi, the Guangxi Zhuang Autonomous Region Patentee before: Cenxi Productivity Promotion Center |
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Application publication date: 20150805 Assignee: YULIN RONGXIAN QICHANG BOAR BREEDING CO.,LTD. Assignor: WUZHOU FOOD AND DRUG INSPECTION INSTITUTE Contract record no.: X2022450000311 Denomination of invention: Simultaneous separation of ractopamine, clenbuterol and salbutamol from pig urine by SLE Granted publication date: 20160629 License type: Common License Record date: 20221212 |