CN104818320B - Primer, probe, detection architecture and the kit of disposable detection lung cancer multiple gene - Google Patents
Primer, probe, detection architecture and the kit of disposable detection lung cancer multiple gene Download PDFInfo
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- CN104818320B CN104818320B CN201410727044.4A CN201410727044A CN104818320B CN 104818320 B CN104818320 B CN 104818320B CN 201410727044 A CN201410727044 A CN 201410727044A CN 104818320 B CN104818320 B CN 104818320B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
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- Proteomics, Peptides & Aminoacids (AREA)
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Material | Concentration | Dosage (μ L) |
Hs-taq enzymes | 5U/μL | 0.25-0.30 |
UNG enzymes | 1U/μL | 0.02-0.06 |
Reverse transcriptase | 0.1-1.0 |
Material | Concentration | Dosage (μ L) |
Hs-taq enzymes | 5U/μL | 0.20-0.25 |
UNG enzymes | 1U/μL | 0.02-0.08 |
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Priority Applications (1)
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CN201410727044.4A CN104818320B (en) | 2014-12-03 | 2014-12-03 | Primer, probe, detection architecture and the kit of disposable detection lung cancer multiple gene |
Applications Claiming Priority (1)
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CN201410727044.4A CN104818320B (en) | 2014-12-03 | 2014-12-03 | Primer, probe, detection architecture and the kit of disposable detection lung cancer multiple gene |
Publications (2)
Publication Number | Publication Date |
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CN104818320A CN104818320A (en) | 2015-08-05 |
CN104818320B true CN104818320B (en) | 2018-02-06 |
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CN201410727044.4A Active CN104818320B (en) | 2014-12-03 | 2014-12-03 | Primer, probe, detection architecture and the kit of disposable detection lung cancer multiple gene |
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Families Citing this family (19)
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CN106801104A (en) * | 2015-11-04 | 2017-06-06 | 深圳市瀚海基因生物科技有限公司 | Multiple PCR primer, kit and purposes |
CN105349654B (en) * | 2015-11-23 | 2019-02-12 | 厦门艾德生物医药科技股份有限公司 | A kind of probe for detecting EGFR genetic mutation, primer, detection architecture and kit |
CN106636442B (en) * | 2017-02-23 | 2020-12-01 | 上海鼎晶生物医药科技股份有限公司 | Human tumor gene variation joint detection kit |
CN106978497A (en) * | 2017-04-26 | 2017-07-25 | 武汉友芝友医疗科技股份有限公司 | Detection primer, probe and the detection kit of EML4 ALK fusion gene mutations |
CN110691852A (en) * | 2017-05-31 | 2020-01-14 | 豪夫迈·罗氏有限公司 | Multiplex PCR detection of ALK, RET and ROS fusions |
KR101987065B1 (en) * | 2017-08-07 | 2019-06-10 | 주식회사 싸이토젠 | A method for analyzing eml4-alk gene variance |
CN107385088A (en) * | 2017-09-04 | 2017-11-24 | 厦门飞朔生物技术有限公司 | A kind of multiple fluorescence PCR detection reagent box for being used to detect mankind's RET Gene Fusions |
CN109504769B (en) * | 2017-09-15 | 2022-06-21 | 益善生物技术股份有限公司 | Specific primer, liquid phase chip kit and method for detecting HER2 gene mutation |
CN108085390A (en) * | 2017-12-29 | 2018-05-29 | 上海桐树生物科技有限公司 | Tumour drives detection agent, detection kit and its application of genetic mutation |
CN110551815A (en) * | 2018-05-30 | 2019-12-10 | 苏州云泰生物医药科技有限公司 | Kit for detecting human Ras gene mutation and using method thereof |
CN110938680A (en) * | 2019-12-25 | 2020-03-31 | 江苏为真生物医药技术股份有限公司 | Gene variation site detection method for simultaneously detecting DNA and RNA |
CN111172281B (en) * | 2019-12-31 | 2023-10-20 | 广州达安基因股份有限公司 | Kit and method for detecting multiple gene mutations of non-small cell lung cancer |
CN110964830A (en) * | 2019-12-31 | 2020-04-07 | 中山大学达安基因股份有限公司 | Kit and method for multiple detection of ROS1 gene mutation |
CN111235272B (en) * | 2020-01-10 | 2023-07-07 | 厦门艾德生物医药科技股份有限公司 | Composition for once detecting multiple gene mutation of lung cancer and application thereof |
CN111139301B (en) * | 2020-03-10 | 2020-12-18 | 无锡市第五人民医院 | Breast cancer related gene ERBB2 site g.39397319C > A mutant and application thereof |
CN112458169A (en) * | 2020-11-25 | 2021-03-09 | 北京科途医学科技有限公司 | Nucleic acid reagent, kit and detection system for HER2 gene mutation detection |
CN113355423B (en) * | 2021-07-07 | 2022-06-07 | 安徽科技学院 | Primer probe and kit for detecting mutation of EGFR gene L858R and application of primer probe and kit |
CN114214412B (en) * | 2021-12-28 | 2024-02-23 | 普瑞斯新(上海)生物医疗科技有限公司 | PIK3CA gene and BRAF gene mutation multiplex detection primer probe and kit thereof |
CN114410791B (en) * | 2022-01-22 | 2024-03-29 | 河南省肿瘤医院 | Method for detecting lung cancer gene fusion based on NanoString platform |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2012170715A1 (en) * | 2011-06-07 | 2012-12-13 | Caris Mpi, Inc. | Molecular profiling for cancer |
WO2014144121A2 (en) * | 2013-03-15 | 2014-09-18 | Life Technologies Corporation | Classification and actionability indices for lung cancer |
-
2014
- 2014-12-03 CN CN201410727044.4A patent/CN104818320B/en active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2012170715A1 (en) * | 2011-06-07 | 2012-12-13 | Caris Mpi, Inc. | Molecular profiling for cancer |
WO2014144121A2 (en) * | 2013-03-15 | 2014-09-18 | Life Technologies Corporation | Classification and actionability indices for lung cancer |
Non-Patent Citations (3)
Title |
---|
A Single-Tube Multiplexed Assay for Detecting ALK,ROS1,and RET Fusions in Lung Cancer;Maruja E. Lira;《The Journal of Molecular Diagnostics》;20140331;第16卷(第2期);229-243 * |
Comparison of targeted next-generation sequencing with conventional sequencing for predicting the responsiveness toepidermal growth factor receptor-tyrosine kinase inhibitor(EGFR-TKI) therapy in never-smokers with lung adenocarcinoma;Ji-Youn Han;《Lung Cancer》;20140429;第85卷(第2期);161-167 * |
Multiplexed Molecular Profiling of Lung Cancer;Hiroaki Akamatsu;《Journal of Thoracic Oncology》;20140731;第9卷(第7期);1048-1052 * |
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Publication number | Publication date |
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CN104818320A (en) | 2015-08-05 |
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Address after: No. 39, Haicang Ding Shan Road, Haicang District, Xiamen, Fujian Patentee after: AMOY DIAGNOSTICS CO., LTD. Address before: 361000 the 5 floor of No. 4 factory, Chuang Chuang center, 289 Weng Jiao Road, Xinyang street, Haicang District, Xiamen, Fujian, China. Patentee before: Amoy Diagnostics Co., Ltd. |
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Effective date of registration: 20200810 Address after: Room 201-202, building 3, 138 xinjunhuan Road, Minhang District, Shanghai Patentee after: Shanghai Xiawei Biotechnology Co.,Ltd. Address before: 361000, No. 39, Ding Shan Road, Haicang District, Fujian, Xiamen Patentee before: Xiamen Aide Biomedical Technology Co.,Ltd. |
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Effective date of registration: 20201216 Address after: No. 39, Haicang Ding Shan Road, Haicang District, Xiamen, Fujian Patentee after: Xiamen Aide Biomedical Technology Co.,Ltd. Address before: Room 201-202, building 3, 138 Xinjun Ring Road, Minhang District, Shanghai Patentee before: Shanghai Xiawei Biotechnology Co.,Ltd. |