CN104815360A - Circulating tumor cell filtration treatment instrument - Google Patents
Circulating tumor cell filtration treatment instrument Download PDFInfo
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- CN104815360A CN104815360A CN201510173002.5A CN201510173002A CN104815360A CN 104815360 A CN104815360 A CN 104815360A CN 201510173002 A CN201510173002 A CN 201510173002A CN 104815360 A CN104815360 A CN 104815360A
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- blood
- tumor cell
- circulating tumor
- pressure meter
- patient
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61M—DEVICES FOR INTRODUCING MEDIA INTO, OR ONTO, THE BODY; DEVICES FOR TRANSDUCING BODY MEDIA OR FOR TAKING MEDIA FROM THE BODY; DEVICES FOR PRODUCING OR ENDING SLEEP OR STUPOR
- A61M1/00—Suction or pumping devices for medical purposes; Devices for carrying-off, for treatment of, or for carrying-over, body-liquids; Drainage systems
- A61M1/36—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits
- A61M1/3679—Other treatment of blood in a by-pass of the natural circulatory system, e.g. temperature adaptation, irradiation ; Extra-corporeal blood circuits by absorption
Abstract
The invention belongs to the technical field of medical apparatuses and instruments, and particularly relates to a circulating tumor cell filtration treatment instrument. The treatment instrument is formed by sequentially connecting an artery pressure meter, a heparin pump, a blood pump, a flow-in pressure meter, a core filtering machine, a degassing system and a vein pressure meter through in-vitro blood pipes. According to the circulating tumor cell filtration treatment instrument, the blood of a patient is guided out through an in-vitro blood circulation system and is connected with the core filtering machine, an carbon nano tube modified by epithelial cell adhesion molecule antibodies is combined with cancer cells, the carbon nano tube combined with the cancer cells is filtered with the semipermeable membrane filtering technology, and finally the filtered blood returns to the body of the patient. By means of the circulating tumor cell filtration treatment instrument, an existing chemoradiotherapy method which causes huge side effects on the patient is replaced, the cancer far end transition probability is effectively reduced, pains of the patient are reduced, and the curing rate of the patient is improved.
Description
Technical field
The invention belongs to technical field of medical instruments, be specifically related to the apparats for treating tumor of a kind of physically based deformation absorption.
Background technology
Malignant tumor (cancer) is by the disease of cell division initiation out of control, has now become the great killer threatening each age groups life.According to " 2012 Chinese tumor registration annual report ", the cancer mortality of China reaches 13%, and in the trend increased year by year.But, the treatment of cancer be always present stage medical science be difficult to the disease of capturing.Cancer is the common name of malignant tumor, and tumor comprises benign tumor (the improper hypertrophy of local cells) and malignant tumor.Namely may be there is far-end transfer in local tumor cell, also tumor cell invades blood circulation (often blood system) at other positions of health and grows, and this transfer is also referred to as " pernicious transfer ", indicates the generation of malignant tumor.Once there is far-end transfer, quite difficulty will be become to treatment of cancer and control.In the clinical treatment of present stage, even if after local tumor excision, tumor cell also has diffusion to develop into the probability of malignant tumor.Therefore, in oncotherapy, the tumor cell how controlling to enter blood system has become the task of top priority.General way is clinically radiotherapy chemotherapy, but can cause huge injury to patient body.At scientific research field, existing research can be taked to injure the tumor cell in less physical means separating blood sample, wherein there is a kind of separation method for epithelial cell tumor (such as: hepatocarcinoma, pulmonary carcinoma, breast carcinoma, the brain cancer, skin carcinoma, nasopharyngeal carcinoma, ovarian cancer, carcinoma of testis, carcinoma of prostate etc.) generally approved: utilize epithelial cell adhesion molecule (epithelial cell Tumor invasion, be present on the tumor cell of epithelial tumour) antibody be combined with blood tumor cell, and then isolate the tumor cell in blood.But these methods can only use in blood sample less in the lab, still cannot remove whole tumor cell in blood, stop the diffusion of tumor cell.
Summary of the invention
In order to remove the tumor cell in blood comprehensively, do not produce radiation to patient body to injure with chemistry simultaneously, the present invention devise a kind of blood of human body is extracted out external, filter after tumor cell, again by the medical apparatus and instruments in the blood input human body after purification, be called that circulating tumor cell filters therapeutic instrument, it is for epithelial cell tumor the most common.
The circulating tumor cell of the present invention's design filters therapeutic instrument, is made up of core filter, arterial pressure meter, venous pressure meter, feed pressure meter, off gas system, heparin pump, blood pump, extracorporeal blood pipeline, shown in Figure 1.Wherein, arterial pressure meter 1, heparin pump 2, blood pump 3, feed pressure meter 4, core filter 5, off gas system 10, venous pressure meter 11 connect successively via extracorporeal blood pipeline; Blood pump 3 is for providing extracorporeal circulation power, heparin pump 2 for instilling the anti-hemostasis-coagulation phenomenon of heparin in blood, core filter 5 is for filtering patient body internal recycle tumor cell, blood after purification, after the bubble detection of off gas system 10 is qualified with the blood pressure detecting of venous pressure meter 11, finally by venous return in patient body.
Various piezometers in this therapeutic instrument, heparin pump, off gas system, blood pump are ripe in clinical blood dialysis treatment to be used, and repeats no more in this.Below the design content of core filter is introduced in detail.Core filter inner chamber is divided into upper chamber 8 and lower cavity 9 two parts by a semipermeable membrane 7, and the aperture of described semipermeable membrane 7 is slightly larger than maximum hemocyte diameter (aperture of general semipermeable membrane 7 is 40-100 μm); Upper and lower cavity is respectively equipped with blood flow entrance, blood stream outlet, and upper chamber 8 inwall is provided with piezoelectric ceramics; Be dissolved with a large amount of multilayer carbon nanotube (Fig. 2) modifying the antibody of epithelial cell adhesion molecule in upper chamber, its diameter and length are all less than 200 μm but are greater than semipermeable membrane aperture.When blood enters core filter upper chamber, under the ultrasonication of piezoelectric ceramics, blood fully mixes with multilayer carbon nanotube, circulating tumor cell in blood is attracted by signal transduction factor antibody, is attached on multilayer carbon nanotube, because carbon nanotube sizes is excessive, not by semipermeable membrane, and blood cell size is less than semipermeable membrane aperture, then unblocked, thus filter out tumor cell in blood.
According to apparatus structure and size, obtain the adsorption effect (Fig. 3) of instrument to tumor cell, when the CNT of tumor cell in filter cavity residual in blood reaches 0.10g/ml, in blood, tumor cell can be filtered, and now active carbon can only filter 60%.In addition, we calculate the absorbability (Fig. 4) of instrument to normal plasma cell, and with the results contrast of other adsorbing materials (active carbon), instrument does not almost adsorb normal blood cell and disturbs.Compare common adsorbing material active carbon, the multiwall nanotube of modified antibodies has better filter capacity, has better specific aim to filtration tumor cell.
After the present invention can better suppress local tumor resection operation, the tumor cell diffusion in vivo of epithelial cell tumor, replaces existing chemicotherapy system human body being had to huge typhoid fever, treats with the minimum biology of injury and physical method.Patient can repeatedly accept to filter treatment, thoroughly removes the tumor cell that may cause pernicious transfer in blood, effectively reduces tumor far-end transition probability, reduces patient suffering, improves patient's cure rate.
Accompanying drawing explanation
Fig. 1: the system schematic (direction of arrow is blood flow direction) of circulating tumor cell filtrator.
Fig. 2: the multilayer carbon nanotube outside drawing being modified with the antibody of epithelial cell adhesion molecule.
Fig. 3: instrument to the adsorption effect of tumor cell, tumor cell effect contrast figure in the CNT of modified antibodies and activated carbon filtration blood.
Fig. 4: run after instrument, instrument to the absorbability of normal plasma cell, tumor cell effect contrast figure in the CNT of modified antibodies and activated carbon filtration blood.
Number in the figure: 1 is arterial pressure meter; 2 is heparin pump; 3 is blood pump; 4 is feed pressure meter; 5 is core filter; 6 is piezoelectric ceramics; 7 is semipermeable membrane; 8 is core filter upper chamber; 9 is core filter lower cavity; 10 is off gas system; 11 is venous pressure meter.12 is multilayer carbon nanotube main body, and 13 is the antibody of multilayer carbon nanotube main body epithelial cell adhesion molecule.
Detailed description of the invention
Below in conjunction with drawings and Examples, implementation process of the present invention is described further.
Shown in Figure 1.Therapeutic instrument of the present invention is connected to form via extracorporeal blood pipeline successively by arterial pressure meter 1, heparin pump 2, blood pump 3, feed pressure meter 4, core filter 5, off gas system 10, venous pressure meter 11; Wherein each kind of piezometer, heparin pump, off gas system, blood pump adopt common equipment in clinical blood dialysis treatment at present, and core filter cavity diameter is at 10cm-5m, and cavity length is at 0.5m-5m.
Before treatment starts, with normal saline injection device pipeline, repeatedly rinse device interior.Now the multilayer carbon nanotube of modified antibodies has been placed in core filter upper chamber.
When treating beginning, carrying out internal arteriovenous fistula operation with it patient and drawing arterial blood with safety.
In system, blood pump is responsible for providing circulation power, heparin pump act as anti-hemostasis-coagulation, blood samples of patients enters core filter cavity through equipment such as arterial pressure meter and feed pressure meters.
Piezoelectric ceramics is opened, and utilizes the ultrasonic multilayer carbon nanotube of blood and modified antibodies that makes fully to merge, and allows the tumor cell in blood be attached on CNT.
Semipermeable membrane filters CNT, allows other compositions in blood pass through smoothly.
Blood after purification removes possible bubble by off gas system, and again by venous pressure meter, pressure index normally refluxes afterwards in patient body.
After entire patient's blood circulation, seance terminates.
After treatment terminates, the CNT of removing, cleaning semipermeable membrane, during next opening of device, the CNT renewed and cleaned semipermeable membrane.
Repeatedly can treat according to needs of patients, thoroughly remove blood inner tumour cell.
Claims (3)
1. circulating tumor cell filters a therapeutic instrument, it is characterized in that being connected to form successively via extracorporeal blood pipeline by arterial pressure meter, heparin pump, blood pump, feed pressure meter, core filter, off gas system, venous pressure meter; Wherein, blood pump is used for providing extracorporeal circulation power, heparin pump is used in blood, instill the anti-hemostasis-coagulation phenomenon of heparin, core filter is for filtering circulating tumor cell in blood samples of patients, blood after purification, after the bubble detection of off gas system is qualified with the blood pressure detecting of venous pressure meter, finally by venous return in patient body.
2. circulating tumor cell according to claim 1 filters therapeutic instrument, it is characterized in that described core filter inner chamber is divided into upper chamber and lower cavity two parts by a semipermeable membrane, the aperture of described semipermeable membrane is slightly larger than maximum hemocyte diameter, and its aperture is 40-100 μm; Upper and lower cavity is respectively equipped with blood flow entrance, blood stream outlet, and upper chamber inwall is provided with piezoelectric ceramics; Be dissolved with the multilayer carbon nanotube of the antibody having modified epithelial cell adhesion molecule in a large number in upper chamber, its diameter and length are all less than 200 μm but are greater than semipermeable membrane aperture.
3. circulating tumor cell according to claim 1 filters therapeutic instrument, and it is characterized in that described core filter cavity diameter is at 10cm-5m, cavity length is at 0.5m-5m.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510173002.5A CN104815360A (en) | 2015-04-14 | 2015-04-14 | Circulating tumor cell filtration treatment instrument |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510173002.5A CN104815360A (en) | 2015-04-14 | 2015-04-14 | Circulating tumor cell filtration treatment instrument |
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| CN104815360A true CN104815360A (en) | 2015-08-05 |
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| CN201510173002.5A Pending CN104815360A (en) | 2015-04-14 | 2015-04-14 | Circulating tumor cell filtration treatment instrument |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2018201862A1 (en) * | 2017-05-04 | 2018-11-08 | 中国药科大学 | Circulating tumor cell capture device |
| CN109385369A (en) * | 2018-11-15 | 2019-02-26 | 中南大学湘雅二医院 | A kind of filtration system that can prevent cell rupture |
| CN109923394A (en) * | 2016-11-11 | 2019-06-21 | 生物外科有限公司 | Filter device |
| WO2020001662A1 (en) * | 2018-06-26 | 2020-01-02 | 成都普瑞康生物科技有限公司 | Rare cell capture system and application thereof |
| CN111225608A (en) * | 2017-06-28 | 2020-06-02 | 魏秉庆 | Carbon nanotube sponge capture circulating tumor cells |
| CN113546232A (en) * | 2020-04-24 | 2021-10-26 | 京东方科技集团股份有限公司 | Therapeutic device and method for preparing cell filter membrane |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109923394A (en) * | 2016-11-11 | 2019-06-21 | 生物外科有限公司 | Filter device |
| WO2018201862A1 (en) * | 2017-05-04 | 2018-11-08 | 中国药科大学 | Circulating tumor cell capture device |
| CN111225608A (en) * | 2017-06-28 | 2020-06-02 | 魏秉庆 | Carbon nanotube sponge capture circulating tumor cells |
| CN111225608B (en) * | 2017-06-28 | 2023-09-15 | 魏秉庆 | Carbon nanotube sponge capturing circulating tumor cells |
| WO2020001662A1 (en) * | 2018-06-26 | 2020-01-02 | 成都普瑞康生物科技有限公司 | Rare cell capture system and application thereof |
| CN109385369A (en) * | 2018-11-15 | 2019-02-26 | 中南大学湘雅二医院 | A kind of filtration system that can prevent cell rupture |
| CN109385369B (en) * | 2018-11-15 | 2021-10-08 | 中南大学湘雅二医院 | Filtering system capable of preventing cell rupture |
| CN113546232A (en) * | 2020-04-24 | 2021-10-26 | 京东方科技集团股份有限公司 | Therapeutic device and method for preparing cell filter membrane |
| CN113546232B (en) * | 2020-04-24 | 2023-10-27 | 京东方科技集团股份有限公司 | Therapeutic equipment and preparation method of cell filter membrane |
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Application publication date: 20150805 |
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