CN104814272B - The application of infructescence of Platycarya strobilacea Sieb.et Zucc. Extract - Google Patents
The application of infructescence of Platycarya strobilacea Sieb.et Zucc. Extract Download PDFInfo
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/70—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in livestock or poultry
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- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The application of infructescence of Platycarya strobilacea Sieb.et Zucc. Extract, specifically infructescence of Platycarya strobilacea Sieb.et Zucc. Extract promotes the application in aquatic products volume increase and prevention and treatment aquatic products disease additive in preparation.The aquatic products is fish or shrimp.The fish is green hata.The infructescence of Platycarya strobilacea Sieb.et Zucc. Extract is Fructus Platycaryae strobilaceac preface water extract.For extract of the present invention using water as Extraction solvent, extraction process is simple, economic, green, environmental protection, safety.There is growth-promoting effect with this plant extract feed additive cultivation green hata, through challenge viral dosage the result shows that the extract feed additive has antibacterial ability to vibrio parahaemolytious.
Description
Technical field
The invention belongs to green scientific culture industry fields, and in particular to the application of infructescence of Platycarya strobilacea Sieb.et Zucc. Extract.
Background technique
Currently, in order to improve food conversion ratio, aquaculture cost is reduced with the fast development of China's culture fishery, it is raw
Efficient, low consumption aquatic feeds are produced, effect of the additive in aquatic feeds is more and more valued by the people.Usually in feed
The drugs such as addition growth accelerator, phagostimulant, antioxidant, immunopotentiator prevent and treat disease to reach the growth for promoting aquatic livestock
Disease, enhancing body immunity, saving nutritional need and other effects.But the drugs such as these chemicals, antibiotic, hormone and its
On the one hand the intracorporal metabolite of animal can generate savings and residual in aquatic products, endanger the health of consumer, on the other hand
Pollution can be generated to environment by being such as discharged into breeding water body, destroy the cultivation balance of water body, the serious sustainable health for restricting aquaculture
Development.It can also cause germ drug resistance and using effect to be deteriorated further, since antibiotics growth accelerator is used for a long time, generate
The problems such as side effect, for these reasons, current many countries, have completely forbidden use to the antibiotic of growth-promoting class, therefore,
It can effective the efficient of substitute antibiotics, safety, green additive there is an urgent need to find exploitation.Plant extracts is at low cost with its
It is honest and clean, easy to process, without drug resistance and medicament residue, Small side effects, significant effect, it is resourceful the advantages that pass by people
Note.
Plant extracts is separated, is purified by a certain kind from plant material using physics, chemistry or biological means
Or the biological micromolecule and macromolecule plant product of plurality of active ingredients composition.Plant extracts contains multiple nutritional components and life
Active substances have both nutrition and two kinds of functions of drug, and according to the difference of plant origin and processing technology, plant extracts also has
Standby respective property and function.Main function has the palatability for improving feed, improves the feed intake of animal；Stimulate digestion liquid point
It secretes, improves animal intestinal tract function；With antibacterial and anti-oxidation function.China's traditional Chinese medicine because of its noresidue, be not likely to produce drug resistance
Property, the concern that aquiculture animal immunity can be enhanced and gradually cause more and more researchers, it would be desirable to from traditional Chinese medicine
In extract effective active composition, to replace part high residue drug.It is believed that Chinese herbal medicine is most possibly to replace antibiotic
Feed addictive.
Fructus Platycaryae strobilaceac preface is the fruit of juglandaceae plant Platycarya strobilacea (Plotytarya strohilacea Sieb et Zuce)
Sequence, infructescence strobiloid, ovate-elliptic is cylindrical to long ellipticity, and long 2.5-5cm, diameter 2-3cm, package piece harbor, and big matter is brown
Color；Pyrene is flat, and two sides have narrow wing.Seed is oval, plants epithelium matter.The ground such as northwest China, East China, Central China, south China produce.
Platycarya strobilacea is civil using wide and with a long history in China, is mostly used as medicine with leaf, infructescence.Fructus Platycaryae strobilaceac preface have it is clearing heat and detoxicating, dissipate
Wind analgesic, the effect of activating microcirculation and removing stasis medicinal, sensible apocenosis, be mainly used for nasosinusitis, headache, internal injury chest is swollen, abdominal pain, arthralgia and myalgia, carbuncle swells,
The illnesss such as scabies.
Change in fragrant infructescence and contains more rich vegatable tannin.Change fragrant fruit tannin and belongs to Ellagitannins, it is its special
Biology and pharmacological activity be its medical value basis.Therefore, the extraction preparation of the fragrant infructescence tannin ingredient of developmentization and
Bioactivity research has important directive significance to the fragrant infructescence resource of better development and utilizationization.Known domestic and foreign literature
Research to fragrant infructescence extract and pharmacological activity is changed, focuses mostly in Platycarya strobilacea and its infructescence extract to cytotoxicity, enteron aisle
Inhibiting effect, antioxidation, pre- anti-cancer and the anti-aging effects of interior harmful bacteria and Platycarya strobilacea are treated as herbal mixture
The report of acute and chronic rhinitis, nasosinusitis, but have no the research about Fructus Platycaryae strobilaceac preface as feeding additive aquatic animal.
Summary of the invention
The technical issues of solution: the object of the present invention is to provide the applications of infructescence of Platycarya strobilacea Sieb.et Zucc. Extract, will disappear containing sterilization
The change perfume (or spice) berry extract of scorching ingredient is produced into feed addictive, and this feed using plant extracts as additive is used to cultivate
Fishes and shrimps growth rate can be improved in the aquatic livestocks such as fish, shrimp, has prevention and therapeutic effect to multiple aquatic animal disease.
Technical solution: infructescence of Platycarya strobilacea Sieb.et Zucc. Extract promotes answering in aquatic products volume increase and prevention and treatment aquatic products disease additive in preparation
With.The aquatic products is fish or shrimp.The fish is green hata.The infructescence of Platycarya strobilacea Sieb.et Zucc. Extract is the extraction of Fructus Platycaryae strobilaceac preface water
Object.The infructescence of Platycarya strobilacea Sieb.et Zucc. Extract is mixed with commercial diet according to the mass ratio of 1:50-200.A kind of aquatic feeds addition
Agent, effective component are infructescence of Platycarya strobilacea Sieb.et Zucc. Extract, and tannin content is not less than 75wt.%.
The utility model has the advantages that extract of the present invention is using water as Extraction solvent, extraction process is simple, economic, green, environmental protection, safety.
There is growth-promoting effect with this plant extract feed additive cultivation green hata, through challenge viral dosage the result shows that the extraction
Object feed addictive has antibacterial ability to vibrio parahaemolytious.
Specific embodiment
This specific embodiment is only the explanation to invention, is not limitation of the present invention, and those skilled in the art are readding
The modification that can make not creative contribution after this specification to the present embodiment as needed is run through, but as long as in power of the invention
All by the protection of Patent Law in sharp claimed range.
Embodiment 1:
By the change perfume (or spice) infructescence raw material 2000g to moisture for 13.2wt% that dries in the shade, sundries is removed, 3-5mm is crushed to, is added
12L pure water solution extracts 1h in 80 DEG C of water-baths, and extracting solution filtering is separated by solid-liquid separation, and repeats to extract twice, discards waste residue,
Combined extract.Extracting solution cooling and standings are settled overnight, discard precipitating, supernatant is concentrated in vacuo, freeze-drying
Fragrant infructescence extract, tannin content are greater than 76%.
Embodiment 2:
By the fragrant infructescence extract of change obtained by embodiment 1 according to the additive amount point of 0.5wt.%, 1.0wt.% and 2.0wt.%
It is not added in commercially available grouper mash feed, fish oil and water is added after mixing and is modulated that (feed amount of water is every
900-100g water is added in 2.5kg feed, and fish oil is added according to 5% ratio of feeding quality score), using twin-screw feed granulating
The hard pellet diet of 1.5mm is made in machine, carries out culture experiment, investigates the feed addictive to the shadow of green hata growth performance
It rings.
Each experimental group sets two repetitions, will test fry and be randomly assigned into the 8 200L rectangle blue for having flowing water modelings
To expect in sink, each sink puts 60 tails in a suitable place to breed, experiment feed 2 times is fed in 8:00 and 16:00 daily, every time until being satiated with food, and
Excrement is removed in time.24 hours inflation, flowing water during raising, water temperature are 21~33 DEG C, dissolved oxygen > 5mg/L, experiment by a definite date 60
It.Experiment takes 50 tails when starting at random, and the body for measuring all survival fishes at the end of experiment is long, after weight, and sample is placed in -20 DEG C
Refrigerator is saved for chemical analysis in the future.
By the raising of 60d, the growth indexes and survival rate measurement result of each group experiment fish are as shown in table 1.As seen from table,
Whole average weight, body weight increase rate and the specific growth rate of experimental group 1 (0.5% addition group of plant extracts) grouper are most
Height, other are successively 3 ﹥ experimental group of experimental group, 2 ﹥ experimental groups 4 (control group).And grouper coefficient of condition is successively from big to small: real
Test group 3 ﹥ experimental group, 4 ﹥ experimental group, 2 ﹥ experimental group 1.From experimental result it can be seen that all addition group groupers of plant extracts are raw
Long performance is superior to control group (no additives), and extract optimum addition is 0.5%.The experiment of being added to perfume infructescence extract
Group grouper survival rate is above control group.Illustrate that this plant extract feed additive cultivation green hata has growth promotion
Effect.
Influence (60d) of the additionization perfume infructescence extract to green hata growth performance in 1 feed of table
Embodiment 3:
It is research vibrio parahaemolytious to the pathogenicity of green hata, first randomly selects 17 tail groupers and be divided into 4 concentration groups,
The bacterium solution (5 μ L/g) of every group of various concentration according to the weight size of fish injection respective volume is to determine its half lethal concentration.Knot
Fruit display injection OD600 value is that 0.17 vibrio parahaemolytious grouper will not be lethal, so 35 times of dilution groups conducts pair will be injected
According to group.It is observed after 72h and determines half lethal concentration, is i.e. OD600 is about 0.6-2.0.
The fragrant infructescence extract of change is added according to 0.5% ratio into feed and cultivates green hata, while control is set
Group (no added group).After raising experiment, it is secondary that two experimental groups take 20 tails health, the complete green hata of body surface to carry out respectively
Hemolysis vibrion attacks poison injection experiment.Vibrio parahaemolytious is resuspended with PBS buffer solution to OD600=1.8, according to the weight of every tail fish
The injection (5 μ L/g) for carrying out respective volume is injected in abdominal cavity under grouper abdomeinal fin, the grouper do not injected as blank group,
Count the cumulative mortality of different disposal group.
The results are shown in Table 2 for challenge viral dosage of the vibrio parahaemolytious to green hata.The 6th day after vibrio parahaemolytious injection
(120h), experimental group (0.5% addition group of extract) grouper cumulative mortality are 20%, substantially less than control group (60%) (P
<0.05).Show that additionization perfume infructescence extract can effectively enhance the antibacterial ability of green hata in feed.
Challenge viral dosage result of 2 vibrio parahaemolytious of table to green hata
The present invention is not limited to the above embodiments, can be implemented described in the content of present invention and has the good result.
Claims (2)
1. infructescence of Platycarya strobilacea Sieb.et Zucc. Extract promotes the application in green hata volume increase and prevention and treatment vibrio parahaemolytious additive in preparation,
It is characterized in that the infructescence of Platycarya strobilacea Sieb.et Zucc. Extract is Fructus Platycaryae strobilaceac preface water extract, tannin content is not less than 75wt.%.
2. application according to claim 1, feature is in the infructescence of Platycarya strobilacea Sieb.et Zucc. Extract and commercial diet according to 1:50-
200 mass ratio mixing.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510225149.4A CN104814272B (en) | 2015-05-05 | 2015-05-05 | The application of infructescence of Platycarya strobilacea Sieb.et Zucc. Extract |
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| CN201510225149.4A CN104814272B (en) | 2015-05-05 | 2015-05-05 | The application of infructescence of Platycarya strobilacea Sieb.et Zucc. Extract |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110050906B (en) * | 2019-01-28 | 2022-08-26 | 广东海洋大学 | Application of hydrolyzed tannin in improving prawn growth performance and prawn intestinal microbial abundance |
| CN110368336B (en) * | 2019-07-16 | 2022-04-12 | 广东海洋大学 | Skin-care lotion containing platycarya strobilacea infructescence as well as preparation method and application of skin-care lotion |
| CN111493222A (en) * | 2020-06-12 | 2020-08-07 | 中国林业科学研究院林产化学工业研究所 | Application of platycarya strobilacea fruit extract in feed for freshwater fish |
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| CN102178730A (en) * | 2011-05-13 | 2011-09-14 | 西北大学 | Platycarya strobilacea sieb infructescence polyphenols extract as well as extracting method and application thereof |
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