CN104800836A - Fish collagen protein as well as production method and application thereof - Google Patents

Abstract

The invention relates to fish collagen proteins as well as a production method and an application thereof. Fish bone collagen, chondroitin sulfate and traditional Chinese medicine preparation are uniformly mixed in a weight ratio of 10: 3: 2; the traditional Chinese medicine preparation is prepared by adopting the following raw materials: ginkgo leaves, prepared rhizome of rehmannia, frankincense, mulberry fruits, deer-horn glue, caulis spatholobi, fructus psoraleae, Tuckahoe, atractylodes, dandelion, Chinese holly, astragalus membranaceus, barbary wolfberry fruit, polygonum multiflorum, Chinese yam, codonopsis pilosula, ligusticum wallichii, acanthopanax roots, radix puerariae, eucommia ulmoides, radix achyranthis bidentatae, dried orange peel, gorgon euryale seed and herba epimedii. All raw materials of the protein powder are compatible with one another, a synergistic effect can be realized, the fish collagen protein is applicable to the osteoporosis, and the prospect is wide.

Description

A kind of fish collagen and production method thereof and application

Technical field

The present invention relates to drug world, relate to a kind of fish collagen, particularly with the fish bone collagen albumen fish collagen protein powder that is primary raw material and production method thereof and application.

Background technology

Osteoporosis and osteoporosis, be one group of osteopathia that many reasons causes, osseous tissue has normal calcification, and calcium salt and substrate are normal rates, and the metabolic osteopathy being reduced to feature with unit volume inner bone tissues amount becomes.In most osteoporosis, caused by the minimizing of osseous tissue increases mainly due to bone absorption.With skeleton pain, be easy to fracture for feature.It is one of social problem and medical problem the most outstanding in the world today, has caused the extensive concern of the United Nations and countries in the world.Osteoporosis can cause bone amount to reduce and bone strength declines, then affects by slight external force or other factors, can fracture, and claims osteoporotic fracture.China is the country that aging population are maximum in the world.Within current more than 60 years old, old man is more than 1.8 hundred million, accounts for 13% of total population, enters aged tendency of population society fast, osteoporosis, patients with osteoporotic bone fracture caused by aged tendency of population society, 84,000,000 people in 2000; 1.4 hundred million people in 2010, account for 7.9% of total population, and sickness rate is obvious increasing trend.

Osteoporosis, osteoporotic fracture main clinical manifestation: soreness of the waist and knees, Leg Cramps, skeleton pain, health shortens, bow-backed deformity, scoliosis, not resistance to fatigue, limbs fatigue, deficiency-heat night sweat etc.Severe patient chest deformity, thoracic cavity diminishes, even occur uncomfortable in chest, breathe hard, the symptom such as cyanosis, dyspnea.Its clinical characters: patient age is large, and body constitution is weak, severe symptoms, and complication is many, and the course for the treatment of is long, and functional rehabilitation is poor, and disability rate is high, and mortality rate is high.Microtrauma can cause vertebral compression fractures, Hip Fracture, radius far-end fracture, proximal humeral fracture, fracture of distal end of humerus, ankle fracture, fracture of the tibial plateau, Methods for Distal Femoral Fractures etc.Because of activity minimizing or long-term bed, easily there is pulmonary infection, urinary system infection, pressure ulcer, deep venous thrombosis of lower limbs, pulmonary infarction, severe complications such as multiple organ dysfunction syndrome and dead.Not only give patient and family bring animally with spiritual great pain, return patient and family brings the huge spending of medical expense and heavy financial burden.

Western medical treatment aspect: some line special-purpose medicaments treatment cycle are long, take effect slow, side effect is large; Some Drugs in Therapy potency ratio is unreasonable, and restrictive condition is many, and drug compliance is low; Osteoporotic fracture operative treatment has a big risk, the factors such as complication is many, somewhat expensive.Some patients is made to receive nonstandard treatment, even have selected therapy discontinued or abandoning cure, occur that in treatment clinical course, patient's bone amount continues to reduce, bone strength continuous decrease, slight external force can cause " returning to poverty " phenomenon of fracture generation or refracture, and patient's disability rate and mortality rate are obviously increased.Have a strong impact on the whole people and prevented and treated osteoporosis engineering progress and fitness-for-all planning implementation, increase the weight of the focus outstanding problem of aged tendency of population society.

Summary of the invention

The object of the invention is the deficiency in order to overcome background technology, in conjunction with a large amount of ancestral side and oneself medical practice for many years, by the research to Chinese medicine, and in conjunction with dialectical demonstration, in many ways collect the length of many families, seek therapeutic regimen, according to philosophic thinking and the Chinese medicine " kidney governing bones; store essential substances, bone growth promoting marrow " of the materialistic dialectics; " liver governing tendons, store blood, main catharsis "; " spleen mainization fortune foodstuff essence, to give birth to and bring up muscle "." asthenia ", " suffering from a deficiency of the kidney ", " suffer from a deficiency of the kidney, bone is become homeless and is supported to the atrophic debility of bones then osteodynia "; " Liver and kidney is insufficiency of the spleen ", " then failure of the spinal column to keep in upright position, bone is withered and marrow is few, sends out as the atrophic debility of bones ", " its muscles and bones is not flourish ", " easily fracturing "." excess of the kidney is bone Animando then ", " bone marrow biochemistry is active, and skeleton nourishes sufficient, firm and strong "." qi being the governor of blood, blood being the material foundation of QI ", " stagnation of QI is blood stasis then, the capable then blood of gas "; ", with behavior honor, blood is to live as expensive for gas "; " stagnation of QI and blood may bring about pain, general rule is not bitterly "; " knot then loose it ", " damage then benefit ", " deficiency syndrome should be treated by tonifying method "; " stasis of blood is gone, newborn, bone close ".Basic theories such as " preventive treatment of disease ".Adhere to the kidney invigorating nourishing the liver, hold concurrently and the five internal organs; Nourishing yin and warming yang, qi and blood tonifying; Body fluid and tune, mend marrow bone growth promoting; Treating both the principal and secondary aspects of a disease.

For achieving the above object, the technical solution used in the present invention is as follows:

With fish collagen be main constituent for an osteoporotic egg albumen powder, its by fish bone collagen egg albumen powder, chondroitin sulfate powder, Chinese medicine preparation according to the weight ratio mix homogeneously of 10:3:2;

Described Chinese medicine preparation is prepared by the raw material of following proportioning and obtains:

Folium Ginkgo, Radix Rehmanniae Preparata, Olibanum, Fructus Mori, Colla cornus cervi, Caulis Spatholobi, Fructus Psoraleae, Poria, the Rhizoma Atractylodis Macrocephalae, Herba Taraxaci, Ilex purpurea Hassk.[I.chinensis Sims, the Radix Astragali, Fructus Lycii, Radix Polygoni Multiflori, Rhizoma Dioscoreae, Radix Codonopsis, Rhizoma Chuanxiong, Radix Et Caulis Acanthopanacis Senticosi, Radix Puerariae, the Cortex Eucommiae, Radix Achyranthis Bidentatae, Pericarpium Citri Reticulatae, Semen Euryales, Herba Epimedii.

Preferably, described Chinese medicine preparation is prepared by the raw material of following weight proportioning and obtains:

Folium Ginkgo 35 parts, Radix Rehmanniae Preparata 35 parts, Olibanum 35 parts, Fructus Mori 35 parts, Colla cornus cervi 30 parts,

Caulis Spatholobi 30 parts, Fructus Psoraleae 30 parts, 30 parts, Poria, the Rhizoma Atractylodis Macrocephalae 28 parts, Herba Taraxaci 28 parts,

Ilex purpurea Hassk.[I.chinensis Sims 25 parts, the Radix Astragali 25 parts, Fructus Lycii 25 parts, Radix Polygoni Multiflori 25 parts, Rhizoma Dioscoreae 22 parts,

Radix Codonopsis 20 parts, Rhizoma Chuanxiong 20 parts, Radix Et Caulis Acanthopanacis Senticosi 20 parts, Radix Puerariae 15 parts, the Cortex Eucommiae 15 parts,

Radix Achyranthis Bidentatae 10 parts, Pericarpium Citri Reticulatae 10 parts, Semen Euryales 10 parts, Herba Epimedii 10 parts.

The preparation method of described Chinese medicine preparation is as follows:

Above-mentioned raw materials medicine is taken for subsequent use according to above-mentioned weight:

(1) get Folium Ginkgo, Ilex purpurea Hassk.[I.chinensis Sims, the Radix Astragali, Radix Polygoni Multiflori, Radix Codonopsis, Semen Euryales, Herba Epimedii mix in described ratio, add the ethanol (volume ratio) relative to mixture 3 times of weight 95%, reflux, extract, 2 times, each 0.5 hour, extracting solution merges, be concentrated into the clear paste that density is 1.2g/ml, 80 DEG C of oven dry, are ground into powder;

(2) get residual components and add 3 times of weight water decoctions twice, 2 hours first times, 1 hour (adding the water of 3 times of weight equally) of second time, merge twice decoction liquor, it is the extractum of 1.2g/ml that drying under reduced pressure obtains density, is ground into powder;

(3) the powder mix homogeneously prepared by step (1) and step (2) and get final product.

Consumption: each 3 grams of egg albumen powder, every day 3 times one month is a course for the treatment of, takes 1-3 the course for the treatment of continuously.

In skeleton, organic 70-80% is collagen protein, when skeleton generates, first sufficient collagen fabric must be synthesized to organize skeletogenous framework, and the aminoacid of collagen protein, hydroxyproline transport the vehicle of calcium to osteocyte in blood plasma, the ossein in osteocyte is the adhesive of calcium hydroxy phosphate.It and calcium hydroxy phosphate together constitute the main body of skeleton.After people enters person in middle and old age, worse and worse, quantity is fewer and feweri for collagen protein quality in skeleton, can not effectively all calcium hydroxy phosphates be binded, at this moment osteoporosis will occur, fish collagen has been proved can via oral and reach the effect of supplementary soft tissue structure.

The present invention, by fish collagen and Chinese medicine preparation mixing, through rational compatibility, has given full play to characteristic property and effect of each raw material self, has taken into account complementary action each other; Through each raw material synergism each other, its drug effect complements each other, and reaches best coupling each other.

The component of medicine of the present invention all adopts natural raw material of Chinese medicine, and its preparation is easy, and medicine source is extensive, and with low cost, it follows the prescriptions principle of the traditional Chinese medical science, and through clinical practice checking, it is reliably evident in efficacy, mild in medicine property and, there is not toxic and side effects, have a extensive future.

Detailed description of the invention

Embodiment 1:

A kind of fish collagen, its by fish bone collagen albumen, chondroitin sulfate, Chinese medicine preparation according to the weight ratio mix homogeneously of 10:3:2;

Described Chinese medicine preparation is prepared by the raw material of following weight proportioning and obtains:

Folium Ginkgo 35 parts, 35 parts, Radix Rehmanniae Preparata, Olibanum 35 parts, Fructus Mori 35 parts, Colla cornus cervi 30 parts,

Caulis Spatholobi 30 parts, Fructus Psoraleae 30 parts, 30 parts, Poria, the Rhizoma Atractylodis Macrocephalae 28 parts, Herba Taraxaci 28 parts,

Ilex purpurea Hassk.[I.chinensis Sims 25 parts, the Radix Astragali 25 parts, Fructus Lycii 25 parts, Radix Polygoni Multiflori 25 parts, Rhizoma Dioscoreae 22 parts,

Radix Codonopsis 20 parts, Rhizoma Chuanxiong 20 parts, Radix Et Caulis Acanthopanacis Senticosi 20 parts, Radix Puerariae 15 parts, the Cortex Eucommiae 15 parts,

Radix Achyranthis Bidentatae 10 parts, Pericarpium Citri Reticulatae 10 parts, Semen Euryales 10 parts, Herba Epimedii 10 parts.

The preparation method of described Chinese medicine preparation is as follows:

Above-mentioned raw materials medicine is taken for subsequent use according to above-mentioned weight:

(1) get Folium Ginkgo, Ilex purpurea Hassk.[I.chinensis Sims, the Radix Astragali, Radix Polygoni Multiflori, Radix Codonopsis, Semen Euryales, Herba Epimedii mix in described ratio, add the ethanol (volume ratio) relative to mixture 3 times of weight 95%, reflux, extract, 2 times, each 0.5 hour, extracting solution merges, be concentrated into the clear paste that density is 1.2g/ml, 80 DEG C of oven dry, are ground into powder;

(2) get residual components and add 3 times of weight water decoctions twice, 2 hours first times, 1 hour (adding the water of 3 times of weight equally) of second time, merge twice decoction liquor, it is the extractum of 1.2g/ml that drying under reduced pressure obtains density, is ground into powder;

(3) the powder mix homogeneously prepared by step (1) and step (2) and get final product.

Consumption: each 3 grams, every day 3 times one month is a course for the treatment of, takes 1-3 the course for the treatment of continuously.

Embodiment 2

Zoopery

Laboratory animal BABc mice 40, male and female half and half, cleaning grade, body weight 20-22g, is provided by the People's Hospital of city animal center.Be divided into 4 groups at random, often organize 10, i.e. blank group 1, model control group 2, embodiment 1 medicine group 3, control drug group 4.Except 1 group gives except normal saline, all the other each group all gives dexamethasone 1mg/kg, injection in every three days once, intramuscular injection, meanwhile, associating retinoic acid gavage, every day consumption 70mg/kg, totally three weeks.

During medication modeling except blank group and model control group give isometric(al) normal saline gavage, the embodiment of the present invention 1 medicine group adopts the administration of gavage mode, dosage is 0.5g/kg, every day three times, control drug group adopts medicine XIANLING GUBAO JIAONANG granule, dosage is 0.5g/kg, every day three times.Administration is after three weeks, detects writhing number of times, then puts to death mice, and the mice neck that breaks gets blood, centrifuging and taking serum, detects S-Ca(serum calcium with automatic biochemistry analyzer) content.See table 1:

Table 1

Group	S-Ca（mmol/L）	Writhing number of times (secondary/15 minute)
			Blank group	2.03±0.17
Model control group	4.15±1.03	55±14
			Embodiment 1 medicine group	2.47±0.51	13±3
Control drug group	2.85±0.67	21±4

As seen from Table 1, model group compares with blank group, and S-Ca raises greatly, and writhing number of times increases, obvious difference, and modeling success is described.The embodiment of the present invention 1 medicine group is obviously better than control drug group, and namely Determination of Calcium in Serum content reduces, and P<0.01, illustrates that calcium loss reduces, and points out medicine of the present invention to possess good analgesic effect.

Embodiment 3

Clinical data

From clinic case, select sufferers of osteoporosis face 131 example, be divided into treatment group and matched group at random, treatment group 66 people, matched group 65 people, in treatment group and the case selected by matched group, medical history, the state of an illness, age, sex are as far as possible quite.

Treatment group gives egg albumen powder prepared by the embodiment of the present invention 1, each 3 grams, every day 3 times, a month course for the treatment of, takes 2 courses for the treatment of continuously;

Matched group adopts conventional medicine XIANLING GUBAO JIAONANG (Tongjitang Pharmacy Co., Ltd., Guizhou).Oral, each 3, every day 2 times, be divided into early, late warm water takes, the course for the treatment of is two months.

Efficacy assessment standard

Diagnostic criteria

1. Western medicine diagnose standard: with reference to " Chinese's osteoporosis suggestion diagnostic criteria (the second original text) " (Chinese osteoporosis magazine, the 6th volume the 1st phase in 2000) of in October, 1999 formulation of Aged in China association's osteoporosis committee Criteria of Osteoporosis subject group

2. tcm diagnosis standard: require to formulate according to the evaluation of " guideline of clinical investigations of new Chinese medicine treatment osteoporosis " of " new Chinese medicine guideline of clinical investigations " (trial version in 2002).Show as: lumbar vertebrae pain, aching and limp few power, walk with difficulty, the vertigo, can not be prudent, body of the tongue or partially red or light, the symptoms such as thin lingual fur or thin white, deep-thready pulse, osteoporosis is shown in above-mentioned disease person.

Efficacy assessment standard:

With reference to the evaluation such as " Chinese osteoporosis magazine ", " practical orthopaedics and tranmstology ".

Cure: clinical symptoms, sign disappear, functional rehabilitation is normal, within more than 1 year, does not recur;

Effective: clinical symptoms, sign obviously alleviate, function recovers normal substantially;

Effective: clinical symptoms, sign part alleviate;

Invalid: treatment more than 30 days continuously, symptom, sign are without change.

Therapeutic outcome is in table 2:

Table 2

Group	Cure	Effective	Effectively	Invalid	Total effective rate
						Treatment group	26	22	11	7	89%
Matched group	10	18	16	21	67%

Visible treatment group compares matched group in cure rate and total effective rate in significant difference.

Embodiment 4

Model case:

Shen, man, 63 years old.Lumbago for many years, time light time heavy, nearly one month aggravation.Lumbago unbearably, and to two hip and numbness of lower limbs pain, can not stand for a long time sitting and normal waking activity, be afraid of cold obviously, and left lower limb is obvious, sleeps not good enough, and diet is general, and urine is normal, and stool is normal by waist lower limb.Give fish collagen protein powder prepared by embodiment 1, after the course for the treatment of, symptom alleviates, and after continuing to take two courses for the treatment of, symptom portion disappears, drug withdrawal observe so far more than a year the state of an illness do not recur, be cure.

Above-mentioned each Chinese medicine that the present invention adopts makes compatibility mutually, its collaborative effect of curing the disease can be played, there is between each composition of raw material of Chinese medicine used drug effect be interweaved and mutually promote and coordinate usefulness, through clinical verification, be applicable to osteoporosis, the Chinese medicine composition of the application is with low cost, evident in efficacy, has broad application prospects.

Claims (3)

1. a fish collagen, is characterized in that, described fish collagen is by fish bone collagen albumen, chondroitin sulfate, Chinese medicine preparation according to the weight ratio mix homogeneously of 10:3:2, and described Chinese medicine preparation is prepared by following raw material and obtains:

Folium Ginkgo, Radix Rehmanniae Preparata, Olibanum, Fructus Mori, Colla cornus cervi, Caulis Spatholobi, Fructus Psoraleae, Poria, the Rhizoma Atractylodis Macrocephalae, Herba Taraxaci, Ilex purpurea Hassk.[I.chinensis Sims, the Radix Astragali, Fructus Lycii, Radix Polygoni Multiflori, Rhizoma Dioscoreae, Radix Codonopsis, Rhizoma Chuanxiong, Radix Et Caulis Acanthopanacis Senticosi, Radix Puerariae, the Cortex Eucommiae, Radix Achyranthis Bidentatae, Pericarpium Citri Reticulatae, Semen Euryales, Herba Epimedii.

2. fish collagen according to claim 1, is characterized in that, described Chinese medicine preparation is prepared by the raw material of following weight proportioning and obtains:

Folium Ginkgo 35 parts, Radix Rehmanniae Preparata 35 parts, Olibanum 35 parts, Fructus Mori 35 parts, Colla cornus cervi 30 parts,

Caulis Spatholobi 30 parts, Fructus Psoraleae 30 parts, 30 parts, Poria, the Rhizoma Atractylodis Macrocephalae 28 parts, Herba Taraxaci 28 parts,

Ilex purpurea Hassk.[I.chinensis Sims 25 parts, the Radix Astragali 25 parts, Fructus Lycii 25 parts, Radix Polygoni Multiflori 25 parts, Rhizoma Dioscoreae 22 parts,

Radix Codonopsis 20 parts, Rhizoma Chuanxiong 20 parts, Radix Et Caulis Acanthopanacis Senticosi 20 parts, Radix Puerariae 15 parts, the Cortex Eucommiae 15 parts,

Radix Achyranthis Bidentatae 10 parts, Pericarpium Citri Reticulatae 10 parts, Semen Euryales 10 parts, Herba Epimedii 10 parts.

3. the fish collagen according to claim 1-2, is characterized in that, the preparation method of described Chinese medicine preparation comprises the steps:

Each raw material for standby is taken according to weight:

(1) get Folium Ginkgo, Ilex purpurea Hassk.[I.chinensis Sims, the Radix Astragali, Radix Polygoni Multiflori, Radix Codonopsis, Semen Euryales, Herba Epimedii mixing, add the ethanol relative to mixture 3 times of weight 95%, reflux, extract, 2 times, each 0.5 hour, extracting solution merged, and is concentrated into the clear paste that density is 1.2g/ml, 80 DEG C of oven dry, are ground into powder;

(2) get surplus stock, mixing, adds the soak by water twice of 3 times of weight, and 2 hours first times, second time 1 hour, merges twice decoction liquor, and it is the extractum of 1.2g/ml that drying under reduced pressure obtains density, is ground into powder;

(3) the powder mix homogeneously prepared by step (1) and step (2) and get final product.