CN104777293A - Detection device and detection method - Google Patents
Detection device and detection method Download PDFInfo
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- CN104777293A CN104777293A CN201510158851.3A CN201510158851A CN104777293A CN 104777293 A CN104777293 A CN 104777293A CN 201510158851 A CN201510158851 A CN 201510158851A CN 104777293 A CN104777293 A CN 104777293A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
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Abstract
The invention provides a detection device. The detection device comprises a sampling tube, a sampler and a reagent bottle, wherein the sampling tube is mounted on the sampler, a reagent is contained in the reagent bottle, the sampler and the reagent bottle can be in sealed fit, when the sampler and the reagent bottle are in sealed fit, the sampling tube is arranged in the reagent bottle and can contact with the reagent. The invention further discloses a detection method. According to the detection device and the detection method, the operation steps are simplified, few amount of the needed sample is needed, good user experience can be provided, adaptability to the range of concentration and content of to-be-detected substance in the sample is great, and interference factors can be reduced by dilution; moreover, the safety and the reliability of analysis process can be improved.
Description
Technical field
The invention belongs to technical field of analytical instruments, relate to a kind of for fluid sample sampling and analyze container and method.
Background technology
In fields such as food, environment, medical treatment, an important step in detecting is analyzed in the sampling of sample.In traditional analysis the sampling of sample and pre-service general operation loaded down with trivial details, the at substantial time, and detection institute's analysis time accounting of core is little.Along with development and the efficient allegro working method of society, have experimental apparatus miniaturization, simple operation, just-in-time of reporting the result analytical approach become inevitable requirement.Therefore, easy sampling and preprocess method have very large value in actual applications.
At present, develop in this respect and have the other diagnostic techniques (Point-of-Care Test, POCT) of bed faster.This refers to analyzes at once in sampling location, saves the complex process program of sample when laboratory inspection, obtains a class new method of assay fast.The ultimate principle of POCT technology is broadly divided into four classes: (1) is infiltrated on the related liquid reagent in classic method in the absorbent material of filter paper and various microporous barrier, become the dried reagent block of integration, then be fixed in hard type matrix, become various forms of diagnostic reagent srip.During detection, make reaction medium with liquid existing in sample, tested composition directly reacts with the dry reagent solidified on carrier.Color reaction is produced, with observing qualitative or instrument detection after adding inspection specimen.Be applicable to whole blood, serum, blood plasma, all kinds of sample such as urine.(2) traditional analysis equipment miniaturization, method of operating is simplified, and making it becomes portable and equipment that is palm-type.(3) said two devices is integrated into unified system.(4) applying biological induction technology, utilizes biological inductor to detect determinand.
POCT is easy and simple to handle, and instrument is easy to carry, can bear traditional experiment function but without the need to traditional experimental facilities.Do not need fixing or special place owing to having, POCT can not limit by time, place, possesses the advantage of round-the-clock 24 hours.Scene is taken or be transported to kit and instrument hand can test on the spot, make POCT can obtain testing result timely.In addition, the easy ease for use of POCT can also reduce time, manpower, the taking of resource aspect, and reduces integrated cost.
POCT has been widely used in the biochemical analysis field of blood and urine.But the POCT technology used at present still has some urgently improvements.One of them problem is that required sample size is bigger than normal.Such as, when detecting with haemoglobin detector, drop of blood directly drops in test strips, and tens of second can obtain testing result, but required blood volume is at 15-20 μ L.For finger tip blood sampling, such blood volume is bigger than normal, and pain obviously.If the required blood volume of test can be reduced, will the pain alleviating person of blood collecting be contributed to, promote Consumer's Experience.In addition, sample pipetting volume amount out of true is the ubiquitous problem of test strips method.In addition, test strips method also has certain requirement for smearing blood, and blood volume is inadequate, and it is inaccurate to smear the unequal result that all can cause.Another problem is that still step is too much for some POCT, there is the space optimized and simplify.Such as current CRP detection kit on the market, is made up of sampling suction nozzle, dilution, test strip.In use, first use suction nozzle pipette samples, then mix with dilution, then dilution is added drop-wise in test strips, then enter to detect.For these class methods, the process of its sample is separated disconnection with detection, simplifies not.
Summary of the invention
The technical matters that first the present invention will solve is to provide a kind of pick-up unit, can facilitate, accurately sample, and and can detect synchronously to get up by sampling.
The technical scheme that technical solution problem of the present invention adopts is: a kind of pick-up unit, comprise stopple coupon, described pick-up unit also comprises sampler and reagent bottle, described stopple coupon is arranged in described sampler, described reagent bottle is built with reagent, and described sampler and described reagent bottle can be sealed and matched, when described sampler and described reagent bottle are sealed and matched, described stopple coupon is in described reagent bottle, and can contact with described reagent.
While employing technique scheme, the present invention can also adopt or combine and adopt following further technical scheme:
Described sampler comprises bottle cap and stopple coupon, and described stopple coupon is fixedly connected on described bottle cap, and described bottle cap can coordinate with the bottle sealing of described reagent bottle.
Described stopple coupon is at least one.
Described reagent bottle comprises body, described body has the plane that at least one pair of is parallel to each other.
When the described plane be parallel to each other is more than a pair, the distance between often pair of plane is entirely not identical.
Described bottle cap is provided with opening.
Another technical matters to be solved by this invention is to provide a kind of detection method, the method application above-mentioned detection device, and comprises the following steps:
1) sampler is adopted to sample testing sample,
2) sampler is covered on body, sample is fully contacted with reagent,
3) pick-up unit is put into instrument to detect.
While employing technique scheme, the present invention can also adopt or combine and adopt following further technical scheme:
Wherein pair of planar on the body of pick-up unit is placed in and detects in light path.
The invention has the beneficial effects as follows: first, simplify operation steps.Substantially sample, synchronously the carrying out of pre-service and detection is achieved.Compared to the method dripped to by sample liquid in test strips, the present invention is more accurately easy.The application of sample amount of test strips method is difficult to control, and drop is too much, very few, smears inequality and all may bring error.And in the present invention, sample liquid can be automatically drawn in pipe under capillary action, simple to operate.Further, the given volume of kapillary, ensure that the accurate of sampling amount of the present invention.Being covered by lid after sampling and mix on body, is carry out along with while reaction again the dilution of sample.Without the need to the further process to sample, directly upper machine just can detect.Therefore, present invention ensures that the accuracy of detection and the simplicity of operation.
Secondly, required amount of samples is few, and Consumer's Experience is better.For blood sample, person of blood collecting has pain in various degree.Reduce required sample size, realize Wicresoft and get blood and contribute to alleviating pain, even when patient almost imperceptible pain complete sampling.But test strips method needs liquid existing in sample to make reaction medium, and tested composition and the dry reagent solidified in papery just can be made to react.Reduction sample volume means the reduction of surveyed area area.And this just proposes very high requirement to the precision of detecting instrument, the spot size such as measuring light beam used is less, and sensitivity is higher, or uses the detection method etc. that detectability is lower.In the present invention, testing process is carried out in the container of pre-filled reagent, and the minimizing of sample volume can not affect the area of surveyed area.Therefore when not promoting existing instruments and methods and being constant, sample volume can be reduced.Get at finger tip the sample sizes such as blood few the present invention there is clear superiority, significantly can promote Consumer's Experience.
Secondly, for testing concentration content range in sample, there is wider adaptive faculty.Such as, when the rectangular configuration formula that component b adopts the equal printing opacity in four sides, there is the logical light face that two groups of light paths are different.When the high signal of test substance content easily overflows, short light path face can be adopted.Otherwise, when determinand content is lower, long light path face can be used, improve sensitivity.Whether, when exceeding standard for a certain index of detection or composition, one of long light path group can be used to lead to light face and detect, serious when running into content overproof, when signal overflows, the detection faces can using light path instead shorter detects.Otherwise, detect a certain index or composition whether up to standard time, can just detect with one of short light path group can be used to lead to light face, too low when running into content, light path can be used instead and improve sensitivity compared with the detection faces of length.
In addition, disturbing factor is decreased by diluting effect.Matrix usually has significant interference to the analytic process analyzing thing, and the accuracy of impact analysis result.Such as, enzymatic or chemical reaction rate can be subject to the impact of the factor such as ionic strength, pH.When in sample, these factors differ greatly, can make a big impact to result.In the present invention, the diluting effect of damping fluid can eliminate the interference that above-mentioned factor is brought, and guarantees the reliable of result.
Finally, the present invention can also improve safety and the reliability of analytic process.Whole testing process is just carried out in an airtight space after sampling, avoid blood sample equal samples to contact with environment, operator and the direct of instrument, stop that sample is contaminated, sample pollutes instrument, and sample is to problems such as the potential pollutions of operator.Greatly improve the security of instrument user.
Accompanying drawing explanation
Fig. 1 is the cut-open view of the sampler of embodiments of the invention 1.
Fig. 2 is the cut-open view of the body of embodiments of the invention 1.
Fig. 3 is the perspective exploded view of embodiments of the invention 1.
Fig. 4 is the skeleton view of the sampler of embodiments of the invention 2.
Fig. 5 is the cut-open view of Fig. 4.
Fig. 6 is the skeleton view of the sampler of embodiments of the invention 3.
Fig. 7 is the cut-open view of Fig. 6.
Fig. 8 is the skeleton view of the body of embodiments of the invention 4.
Fig. 9 is the sectional side elevation of Fig. 8.
Figure 10 is the cross-sectional figure of Fig. 8.
Figure 11 is the skeleton view of the body of embodiments of the invention 5.
Figure 12 is the sectional side elevation of Figure 11.
Figure 13 is the cross-sectional figure of Figure 11.
Figure 14 is the sectional side elevation of the body of embodiments of the invention 6.
Figure 15 is the sectional side elevation of another angle of Figure 14.
Figure 16 is the detection visual angle figure of Figure 14.
Figure 17 is the haemoglobin examination criteria curve of embodiment 7.
Figure 18 is the haemoglobin examination criteria curve of embodiment 8.
Figure 19 is the glucose detection typical curve of embodiment 10.
Embodiment
Embodiment 1, with reference to accompanying drawing 1-3.
Pick-up unit of the present invention comprises sampler and reagent bottle, sampler comprises bottle cap 1 and stopple coupon 3, stopple coupon 3 is fixedly connected on the inside surface of bottle cap 1 by web member 2, reagent bottle comprises body 4, is preinstalled with solid-state or liquid reagent in body 4, and bottle cap 1 can be sealed and matched with the bottleneck of body 4, such as adopt and be threaded, when sampler and reagent bottle are sealed and matched, stopple coupon 3 is in the body 4 of reagent bottle, and can contact with reagent wherein.
Each sampler at least comprises a stopple coupon 3, and in the present embodiment, stopple coupon 3 is 1.
Body 4 has the plane that at least one pair of is parallel to each other, this (a bit) is detection light path to the distance between plane, when detecting, this being placed in plane in the detection light path in instrument, can detecting.
Embodiment 2, with reference to accompanying drawing 4,5.
In the present embodiment, the bottle cap 1 of sampler adopts circle, and bottle cap 1 is provided with perforate 5, perforate 5 is connected with outside kapillary 6, and be beneficial to follow-up possible further analysis, other embodiments of the present embodiment are identical with embodiment 1.
Embodiment 3, with reference to accompanying drawing 6,7.
In the present embodiment, the bottle cap 1 of sampler adopts square, and bottle cap 1 is provided with perforate 5, perforate 5 is connected with outside kapillary 6, and be beneficial to follow-up possible further analysis, other embodiments of the present embodiment are identical with embodiment 1.
Embodiment 4, with reference to accompanying drawing 8-10.
In the present embodiment, the cross section of body 4 is rectangle, has two to the plane be parallel to each other, distance between two planes is not identical, can make two light paths and use, the bottleneck of body 4 have ring-type installation position, suitable with the bottle cap 1 of sampler, ring-type installation position is communicated with body 4.Other embodiments of the present embodiment are identical with embodiment 1.
Embodiment 5, with reference to accompanying drawing 11-13.
In the present embodiment, ring-type installation position is solid position, and other embodiments of the present embodiment are identical with embodiment 5.
Embodiment 6, with reference to accompanying drawing 14-16.
In the present embodiment, body 4 has the plane of recessed two sides symmetry, distance between the plane that this two sides piles is less than the distance between two facial planes without recessed position, make body 4 has at least two different light paths, when detecting, select the light path be applicable to according to the different demands of detected sample.
Embodiment 7, the mensuration 1 of haemoglobin in blood, with reference to accompanying drawing 17.
In the present embodiment, kapillary volume 2uL, the light path 6mm of body 4.Get 0.7mL TritonX-100 and 0.6g sodium dodecylsulphonate, with pH7.2, the phosphate solution of concentration 0.33mmol/L is mixed with 1L test solution.The above-mentioned test solution of 200 μ L is pre-installed, with diaphragm seal bottleneck in bottle.During use, puncture sealing film with suction nozzle, then draw blood sample, build rear upset body, repeatedly put upside down and make it mixing for 10 times, measure under 540nm wavelength.
Embodiment 8, the mensuration 2 of haemoglobin in blood.
In the present embodiment, kapillary volume 3uL, the light path 6mm of body 4.450 μ L reactant liquors are pre-installed, wherein containing the Triton X-100 of 0.1 mol/L NaOH and 25g/L, with diaphragm seal in bottle.During use, puncture sealing film with suction nozzle, then draw blood sample, build rear upset body, make it mixing, measure under 575nm wavelength after 1min.
Embodiment 10, the mensuration 3 of haemoglobin in blood.
In the present embodiment, kapillary volume 2uL, the light path 10mm of body 4.500 μ L reactant liquors are pre-installed, wherein containing the Triton-100 of 20 g/L, the Na of 21.2 g/L in bottle
2cO
3, with film seal.During use, puncture sealing film with suction nozzle, then draw blood sample, build rear upset body, make it mixing, measure under 540nm wavelength.
Embodiment 11, the mensuration of glucose content in beverage.
In the present embodiment, kapillary volume 1uL, the light path 3mm of body 4.In bottle, pre-install 150uL reactant liquor, wherein reagent content is glucose dehydrogenase 20000U/L, mutarotase 1600U/L, nicotinamide adenine dinucleotide 4.7mmol/L, tetrazolium bromide 0.38mmol/L.During use, puncture sealing film with suction nozzle, then draw drink sample, build and mix, measure under 600nm wavelength after 4min.
Embodiment 12, the mensuration of protein content in beverage.
In the present embodiment, kapillary volume 5uL volume, the light path of body 4 is 10mm.In bottle, pre-install 500uL reagent, its composition is often liter of sulfur acid copper 1.5g, sodium potassium tartrate tetrahydrate 6g, NaOH 30g, potassium iodide 1g.During use, puncture sealing film with suction nozzle, then draw drink sample, build and mix, measure under 540nm wavelength.
For haemoglobin, glucose, protein content in embodiment, describe the present invention.But the present invention goes for the detection of other compositions in biochemistry and food analysis equally.The kapillary volume mentioned in literary composition and the light path of body, be only the content selected by part embodiment.Foregoing is not meant to limit the present invention in any manner, and on the contrary, will carry out many amendments, change and change within the scope of the invention.
Claims (8)
1. a pick-up unit, comprise stopple coupon, it is characterized in that: described pick-up unit also comprises sampler and reagent bottle, described stopple coupon is arranged in described sampler, described reagent bottle is built with reagent, and described sampler and described reagent bottle can be sealed and matched, when described sampler and described reagent bottle are sealed and matched, described stopple coupon is in described reagent bottle, and can contact with described reagent.
2. a kind of pick-up unit as claimed in claim 1, it is characterized in that: described sampler comprises bottle cap and stopple coupon, described stopple coupon is fixedly connected on described bottle cap, described bottle cap can coordinate with the bottle sealing of described reagent bottle.
3. a kind of pick-up unit as claimed in claim 1, is characterized in that: described stopple coupon is at least one.
4. a kind of pick-up unit as claimed in claim 1, is characterized in that: described reagent bottle comprises body, described body has the transparent flat that at least one pair of is parallel to each other.
5. a kind of pick-up unit as claimed in claim 4, is characterized in that: when the described transparent flat be parallel to each other is more than a pair, the distance between often pair of plane is entirely not identical.
6. a kind of pick-up unit as claimed in claim 2, is characterized in that: described bottle cap is provided with opening.
7. a detection method, is characterized in that: the pick-up unit of described detection method application according to any one of claim 1-6, and comprises the following steps:
1) sampler is adopted to sample testing sample,
2) sampler is covered on body, sample is fully contacted with reagent,
3) pick-up unit is put into instrument to detect.
8. a kind of detection method as claimed in claim 1, is characterized in that: in described step 3), the wherein pair of planar on the body of pick-up unit is placed in and detects in light path.
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| CN201510158851.3A CN104777293A (en) | 2015-04-05 | 2015-04-05 | Detection device and detection method |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510158851.3A CN104777293A (en) | 2015-04-05 | 2015-04-05 | Detection device and detection method |
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| CN104777293A true CN104777293A (en) | 2015-07-15 |
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| CN201510158851.3A Pending CN104777293A (en) | 2015-04-05 | 2015-04-05 | Detection device and detection method |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109782002A (en) * | 2019-03-27 | 2019-05-21 | 苏州康和顺医疗技术有限公司 | A kind of household specific protein analyzer one adopts one and puts detection method |
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| US20100032437A1 (en) * | 2004-12-17 | 2010-02-11 | Dynamic Systems Gmbh | Container with Transponder |
| CN101883527A (en) * | 2007-11-12 | 2010-11-10 | 莱夫阿塞斯有限公司(上市公司) | Device for biochemical processing and analysis of a sample |
| CN102083955A (en) * | 2008-05-13 | 2011-06-01 | 3M创新有限公司 | Sampling devices and methods of use |
| CN102639246A (en) * | 2009-10-22 | 2012-08-15 | 吉哈德·波耐科 | Test set for a photometric measuring device and photometric measuring method for a sample liquid |
| CN203011829U (en) * | 2012-11-20 | 2013-06-19 | 北京雪迪龙科技股份有限公司 | Cuvette, water quality concentration detecting device and water quality monitoring system |
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2015
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Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1910438A (en) * | 2004-01-21 | 2007-02-07 | 欧雷恩诊断公司 | Sampling and assay device |
| US20100032437A1 (en) * | 2004-12-17 | 2010-02-11 | Dynamic Systems Gmbh | Container with Transponder |
| CN101883527A (en) * | 2007-11-12 | 2010-11-10 | 莱夫阿塞斯有限公司(上市公司) | Device for biochemical processing and analysis of a sample |
| CN102083955A (en) * | 2008-05-13 | 2011-06-01 | 3M创新有限公司 | Sampling devices and methods of use |
| CN201368842Y (en) * | 2009-02-20 | 2009-12-23 | 程俊彪 | Capillary flow cuvette |
| CN102639246A (en) * | 2009-10-22 | 2012-08-15 | 吉哈德·波耐科 | Test set for a photometric measuring device and photometric measuring method for a sample liquid |
| CN203011829U (en) * | 2012-11-20 | 2013-06-19 | 北京雪迪龙科技股份有限公司 | Cuvette, water quality concentration detecting device and water quality monitoring system |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109782002A (en) * | 2019-03-27 | 2019-05-21 | 苏州康和顺医疗技术有限公司 | A kind of household specific protein analyzer one adopts one and puts detection method |
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