CN104774819A - Xylanase particles - Google Patents

Xylanase particles Download PDF

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Publication number
CN104774819A
CN104774819A CN201510141335.XA CN201510141335A CN104774819A CN 104774819 A CN104774819 A CN 104774819A CN 201510141335 A CN201510141335 A CN 201510141335A CN 104774819 A CN104774819 A CN 104774819A
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China
Prior art keywords
particle
zytase
core
coating
granulation
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Inventor
S·A·M·马丁斯
E·S·马克森
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Novo Nordisk AS
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Novo Nordisk AS
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Publication of CN104774819A publication Critical patent/CN104774819A/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K40/00—Shaping or working-up of animal feeding-stuffs
    • A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K40/00—Shaping or working-up of animal feeding-stuffs
    • A23K40/30—Shaping or working-up of animal feeding-stuffs by encapsulating; by coating
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/98—Preparation of granular or free-flowing enzyme compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Food Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Husbandry (AREA)
  • Fodder In General (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

The invention provides a xylanase particle. During the vapour treatment process, the xylanase particle is made optionally, the thermostability of the xylanase particle is better than that of a commercialized product Ronozyme WX (CT) in the prior art. The particle comprises Ronozyme WX, and does not comprise expensive cellulosic fiber, and therefore the production cost is reduced.

Description

Zytase particle
Invention field
The present invention relates to zytase particle and manufacturing the purposes in animal-feed with them, described manufacture, by steam treatment, then is optionally carried out granulation to carry out.It also relates to the method producing zytase particle.
Background of invention
Zytase is used in animal-feed by dawn known in the art, to improve feed utilisation.RonozymeWX zytase is a kind of animal-feed zytase of single component, it derives from thermophilic mould/thin thermophilic hyphomycete of cotton like (Thermomyces lanuginosus) of fine, soft fur, and commercial can from DSM NutritionalProducts, Switzerland obtains.Being described in WO 96/23062 of this zytase and the purposes in animal-feed thereof.
The steam treatment of animal-feed, then optionally carries out granulation, through being commonly used to the digestibility of raising feed and killing salmonella (Salmonella) bacterium, if it exists.This generally includes the steam treatment of more than 80 DEG C.
The Ronozyme WX commercial CT-of can be used as particle (cated heat-staple) obtains, and survives in the high feed manufacturing temperature that its known thermostability with excellence also can exist in steam treatment process.Ronozyme WX (CT) is a kind of T-particle (producing as described in US 4106991) containing cellulosic fibre, and applies (as described in WO 92/12645) with hydrogenated palm kernel oil.
Summary of the invention
Present inventors have developed zytase particle, it has thermostability in steam treatment (then optionally granulation) process, and this is similar or better to business-like prior art Horizontal production Ronozyme WX (CT).Described particle contains Ronozyme WX, and not containing cellulose fiber and/or not containing coating (coating), thus reduce material cost and production cost.
Therefore, the invention provides the particle comprising zytase, wherein said zytase has the aminoacid sequence having at least 90% identity with Ronozyme WX, and wherein said particle not containing cellulose fiber.
The present invention is also provided for the method manufacturing feed combinations, comprises the steps:
A. feed ingredient is mixed with zytase particle,
B. composition (a) described in steam treatment, and
C. optionally described composition (b) is carried out granulation.
Wherein zytase has the aminoacid sequence having at least 90% identity with Ronozyme WX, and wherein particle not containing cellulose fiber and/or be uncoated.
Finally, the invention provides the method for several production zytase particle.
Detailed Description Of The Invention
Zytase
Described zytase is Ronozyme WX, or has the variant of the aminoacid sequence having at least 90% identity with Ronozyme WX.Ronozyme WX has the mature sequence (it is identical with the SEQ ID NO:2 in WO 96/23062) as shown in the residue 32-225 of SEQ ID NO:1.WO 96/23062 also discloses the method for producing and reclaiming Ronozyme WX.Some variants of Ronozyme WX sequence be described in WO 01/66711.
Sequence iden can be specially at least 95%, at least 98% or at least 99%.Identity between two aminoacid sequences uses Needleman-Wunsch algorithm (Needleman and Wunsch, 1970, J.Mol.Biol.48:443-453) as EMBOSS routine package (EMBOSS:The European MolecularBiology Open Software Suite, Rice etc., 2000, Trends Genet.16:276-277) in Needle program (preferred 5.0.0 version or upgrade version) in implement determine.The parameter used is gap open penalty (gap open penalty): 10, breach expansion point penalty (gap extension penalty): 0.5, and EBLOSUM62 (the EMBOSS version of BLOSUM62) permutation matrix (substitution matrix).The output being labeled as the Needle of " the longest identity " (obtaining with nobrief option) uses as percentage identities, and calculates as follows:
(identical residue x 100)/(the breach sum in comparison Chang Du – comparison)
Zytase particle
Zytase particle is particularly suitable for steam treatment, then optionally carries out granulation.They are not containing cellulosic fibre disclosed in US4106991 and WO 92/12645.Avoid using cellulosic fibre to contribute to reducing material cost and tooling cost.Zytase particle is produced by many methods, as rotary drum granulation (drum granulation) (high shear granulation), fluid bed granulation or be used in there is high absorbent capacity core in absorb.Particle can be (coated) or uncoated (uncoated) of coating.
Particle can have matrix (matrix) structure, and wherein by component evenly (equably) mixing, or it can be the particle of layering, and described particle comprises core (core) and surrounds one or more layers of core.
matrix granule (matrix granules)
In a specific embodiment, zytase is present in uniformly in (homogeneous) matrix.The matrix comprising zytase can comprise other auxiliary components.
Matrix granule uses powdered mixture and granulation liquid to produce by rotary drum granulation (mixing tank granulation).Except zytase, powdered mixture and/or granulation liquid can comprise solid-filling agent (carrier), granulation binders and liquid granulating agent.Solid-filling agent can comprise sodium sulfate, calcium carbonate, gypsum (calcium sulfate), other inorganic salt (water-soluble or insoluble) and/or starch.Tackiness agent can comprise oligosaccharides as dextrin.Liquid granulating agent can be water.Rotary drum granulation carries out the particle producing low porosity (porosity) usually under high shear.
The suitable particle size of uncoated particle is 20-2000 μm, is more specifically 50-1000 μm or 250-1000 μm.
inertia core particle (inert core particle):
Inertia core particle is as placebo particle, carrier particle, inactive core (nuclei), inactive particle, incomparable particle (non-pareil particles), inactive particle or seed, be the particle not comprising zytase or only comprise zytase in a small amount, can will comprise the coating mix coating (layered) of zytase on described particle.Their available organic or inorganic materials (if inorganic salt, sugar, sugar alcohol, little organic molecule are if the flour of organic acid or salt, starch, flour, process, Mierocrystalline cellulose, polysaccharide, mineral are as clay or silicate or these the combination of two kinds or more) are prepared.
In a specific embodiment, example to be coated is inactive particle.In a more particular embodiment, the material of core particle is selected from lower group: inorganic salt, sugar alcohol, little organic molecule, starch, flour, Mierocrystalline cellulose and mineral.
Inert particle produces by multiple granulation technique, comprising: crystallization, precipitation, pot coating (pan-coating), fluidized bed coating process, fluid bed agglomeration (fluid bed agglomeration), rotary-atomizing, extrude (extrusion), granulating (prilling), round as a ball (spheronization), size reduce method (sizereduction methods), rotary drum granulation and/or high shear granulation.
additional granulating agent
Particle can comprise additional materials as tackiness agent, weighting agent, filamentary material, stablizer, solubilizing agent, suspension agent, viscous regulator, light spheroid (light spheres), softening agent, salt, lubricant and perfume compound.
tackiness agent
Tackiness agent can be synthetic polymer, wax, fat, fermented liquid, carbohydrate, salt or polypeptide.
Suitable synthetic polymer comprises particularly Polyvinylpyrolidone (PVP) (PVP), polyvinyl alcohol (PVA), polyvinyl acetate (polyvinyl acetate), polyacrylic ester (polyacrylate), polymethacrylate (polymethacrylate), polyacrylamide (polyacrylamide), polysulfonates (polysulfonate), polycarboxylate (polycarboxylate), and their multipolymer, particularly water miscible polymkeric substance or multipolymer.
In a specific embodiment, synthetic polymer is vinyl polymer.
In a specific embodiment, tackiness agent is polypeptide.Described polypeptide is optional from gelatin, collagen, casein, chitosan, poly aspartic acid and polyglutamic acid (poly glutamatic acid).In another embodiment, tackiness agent be derivatived cellulose as hydroxypropylcellulose, methylcellulose gum or CMC.Suitable tackiness agent be carbohydrate binder as sucrose or dextrin, such as Glucidex 21D or Avedex W80.
weighting agent
Suitable weighting agent is water-soluble and/or insoluble inorganic salt, as fine grinding alkali metal sulfates (alkali sulfate), alkaline carbonate (alkali carbonate) and/or alkali metal chloride (alkalichloride), clay as kaolin (such as , English China Clay (China Clay)), wilkinite, talcum, zeolite, chalk, calcium carbonate and/or silicate.Typical weighting agent is sodium sulfate and calcium lignin sulphonate.Other weighting agents are silicon-dioxide, gypsum, kaolin, talcum and neusilin.
absorption in core
Zytase particle produces, as described in WO97/39116 by being absorbed in core with high absorbent capacity.
Core relevant in the context of the invention preferably can absorb the water of at least 10%w/w (based on core weight), more preferably at least 15%w/w, also more preferably at least 20%w/w.Particularly preferred core is the core of the water that can absorb at least 30%w/w, if absorb the core of at least 33%w/w.Some preferred core type has even higher water-retaining capacity (such as the water of about 40%w/w or more).
In the context of the invention, preferred core type comprises the core comprising starch and/or treated starch, especially containing total amount at least 25%w/w (based on core weight), as at least starch of 50%w/w and/or the core of treated starch.
It is suitable (as starch self in the context of the present invention that starch (naturally occurring starch) from a variety of plant origin demonstrates, or as the starting point of treated starch), and relevant starch comprises from following starch: cassava [especially from bitter cassava (Manihot esculenta) or sweet cassava (Manihotdulcis)]; Sago (sago belongs to kind of (a Metroxylon spp.), as sago (M.sagu)); Potato (potato (Solanum tuberosum)); Rice (Some of Oryza Species (Oryza spp)); Corn (Zea mays, Zea mays (Zea mays)); Wheat (Triticum kind (Triticum spp.)); Barley (Hordeum kind (Hordeumspp.), as H.vulgare); Sweet potato (sweet potato (Ipomoea batatas)); Chinese sorghum (sorghum kind (Sorghumspp.)); With Chinese yam (Wild yam kind (Dioscorea spp.)).
The content of enzyme (calculating as pure enzyme protein) typically in the scope of about 0.5%-20%, by the weighing scale of enzyme containing granule.
Coating
Particle can be uncoated/non-applied layer, or they can comprise one or more coating.
Suitable coated material is hydrophobic substance and salt.The coating of protectiveness can comprise further tackiness agent as previously described those.
The amount of coating can form core (uncoated particle) by weight at least 10%, at least 20%, at least 30% or at least 40%.The amount of described coating can form core (uncoated particle) and be less than 100% by weight, is less than 80% or be less than 60%.
hydrophobic coating
Coating can contain hydrophobic substance, and described hydrophobic substance has the fusing point of more than 35 DEG C.Described fusing point can be more than 40 DEG C or more than 45 DEG C.Described fusing point can lower than 100 DEG C, lower than 95 DEG C, lower than 90 DEG C, lower than 85 DEG C, lower than 80 DEG C or lower than 75 DEG C.
Hydrophobic substance has the water contact angle of more than 90 °.Hydrophobic substance is insoluble in water, but is solvable in organic non-polar solvent.
Hydrophobic substance can be fat of triglyceride or wax.The example of fat of triglyceride be hydrogenated vegetable oil or animal grease as beef (ox) grease of hydrogenation, hydrogenated palm kernel oil, hydrogenated cottonseed oil and/or hydrogenated soybean oil, wherein carbon=carbon double bond is converted into carbon-carbon single bond wholly or in part.
Wax close to envrionment temperature is being plasticity (plastic).They can be naturally occurring or synthesis.Naturally occurring wax comprises animal, plant and mineral wax.
Wax or fatty coating can comprise nearly 80%, and be preferably the weighting agent of 60-75%, it is dry powder or any material, is preferably inorganic materials, is more preferably kaolin, Magnesium Silicate q-agent or calcium carbonate.The weighting agent of instruction is incorporated to coating agent with the amount of instruction, and this can reduce particle separately and mutually adheres to and adhere to the trend on granulating device.
salt coating
Salt can be water miscible, specifically to have in 100g water at least 0.1 gram at 20 DEG C, preferably every 100g water at least 0.5g, such as every 100g water at least 1g, such as the solubleness of every 100g water at least 5g.
Salt in coating has more than 81% at 20 DEG C, or the constant humidity of more than 85% or more than 90%, or it can be the another kind of hydrate forms of this salt (such as anhydride (anhydrate)).Salt coating can according to WO 00/01793.
An object lesson of the salt be applicable to is Na 2sO 4(CH 20 DEG C=93%), anhydrous sodium sulphate is specially.
In coating, the content of salt can be at least 60%, at least 70%, at least 80%, at least 90% or at least 95% by weight.
Preferably, described salt uses as salts solution, such as, use fluidized-bed.
The manufacture of feed granules (pellet)
In the manufacture of feed granules, preferably relate to steam treatment before pelletizing, one is called the technique of adjustment (conditioning).In granulation step subsequently, feed is forced through mould and the thread (strands) of gained is cut into the suitable granule of different lengths.In this regulating step process, processing temperature can rise to 60-100 DEG C.
Fodder mixtures (mass (mash feed)) is by mixing the particle comprising feed zytase with required feed ingredient and preparing.Mixture is imported setter, such as, has the stepwise mixing tank (cascade mixer) of steam injection.Feed is heated to assigned temperature by injecting steam in the regulators, 60-100 DEG C, and such as 60 DEG C, 70 DEG C, 80 DEG C, 90 DEG C or 100 DEG C, described temperature is measured in the exit of setter.The residence time can change to several minutes even a few hours from the several seconds.As 5 seconds, 10 seconds, 15 seconds, 30 seconds, 1 minute, 2 minutes, 5 minutes, 10 minutes, 15 minutes, 30 minutes and 1 hour.In a specific embodiment, temperature is 100 DEG C and the residence time is 60 seconds.
In a specific embodiment, the processing temperature in steam treatment process is at least 60 DEG C.In a more particular embodiment, the processing temperature in steam treatment process is at least 70 DEG C.In one even more particular embodiment, the processing temperature in steam treatment process is at least 80 DEG C.In a most specific embodiment, the processing temperature in steam treatment process is at least 90 DEG C.
Feed imports press (press) from setter, such as Simon Heesen press, and is pressed into the particle with different lengths (such as 15mm).After compacting, particle is placed in air-cooler and cools one specific period, such as 15 minutes.
Specific embodiment is the method for the manufacture of feed composition, comprises the following steps:
I. feed ingredient is mixed with particle,
Ii. composition (i) described in steam treatment, and
Iii. optionally by described composition (ii) granulation.
Animal-feed
The feature of particle allows it to be used as the component of animal-feed, and it is through steam treatment and optionally granulation.A specific embodiment is through the feed composition comprising particle of steam treatment and granulation, and described particle comprises zytase.Term " animal " comprises all animals.The example of animal is non-ruminant animal and ruminating animal.
Embodiment
Embodiment 1: the preparation of the Ronozyme WX particle of uncoated cellulose-less
There is the powdered mixture of following composition
0.750kg calcium carbonate
0.750kg dextrin, Avedex W80
12.610kg grinding Na 2sO 4
? in mixing tank FM 50F be granulated by the granulation liquid formed as follows
0.750kg dextrin, Avedex W80
1.400kg Ronozyme WX
1.373kg water
Granulation is by United States Patent (USP) 4,106,991, implements described in embodiment 1.
In fluidized bed dryer by particle drying to water-content lower than 1%, and sieve to obtain the product that granularity is 250-1000 μm.
Embodiment 2: with the Ronozyme WX particle of hydrogenated palm kernel oil, calcium carbonate and kaolin coating cellulose-less in coating mixing tank
The particle of 2,000kg embodiment 1 is placed in 5L mixing tank.
By following coated materials in core:
1. the hydrogenated palm kernel oil of 10% fusing
2. 11% calcium carbonate
3. 11% kaolin
Coating is carried out as follows.First the hydrogenated palm kernel oil of the fusing of (per-cent of uncoated T-particle) 8% (w/w) is sprayed onto on mixture, then adds calcium carbonate and the kaolin 1:1 mixture of 22% (w/w).Coating flow process is to terminate with the final coating of 2% hydrogenated palm kernel oil.
After coating, warm cated T-particle of executing cools in envrionment temperature with air in fluidized-bed.In this technological process, fines is removed.
The cated T-particle of executing containing zytase of cooling carries out classification finally by screening, to guarantee that granularity is 300 μm-1180 μm.
Embodiment 3: with the preparation of the Ronozyme WX MG-particle of 40% salt coating
The sodium sulfate core being 250-500 μm by 4kg screening is loaded into the fluidized-bed of bottom spray system.
Use as left the bed setting in coating procedure:
Air-flow: 200m 3/ hour
Entering air temperature: 100 DEG C
Product temperature: 64 DEG C
1.2mm nozzle: 2.8 bar (bar) nozzle pressure
Sodium sulfate core is sprayed with following mixture:
0.37kg Ronozyme WX
0.01kg dextrin, Avedex W80
1.4kg water.
After coating, by particle drying 5 minutes.
The particle of 3kg is undertaken applying (namely coating accounts for 40% of uncoated core) by 40% salt coating, further as described in WO 2000/001793 and WO 2006/034710:
1.2kg grinds sodium sulfate
2.8kg water
After coating, by particle drying 5 minutes.
By the agglomerate that pellet through sieves is greater than 600 microns to remove any granularity.
Embodiment 4: be absorbed into cassava core and the Ronozyme WX applied with hydrogenated palm kernel oil, calcium carbonate and kaolin.
The tapioca (flour) core (from Agro Comercial, Brazil) of 10kg is imported 50L mixing tank.
By the Water spray core of Ronozyme WX and 0.637kg of 0.96kg, as described in WO 1997/039116.
Core is transferred to fluidized-bed and at 80 DEG C of dry 30min, is then sieved to 250-1180 μm to remove larger agglomerate.
This product of 2.000kg is placed in 5L mixing tank.
By following coated materials in core:
1. the hydrogenated palm kernel oil of 10% fusing
2. 11% calcium carbonate
3. 11% kaolin
Coating is carried out as follows.First the hydrogenated palm kernel oil of the fusing of (per-cent of uncoated T-particle) 8% (w/w) is sprayed onto on mixture, then adds calcium carbonate and the kaolin 1:1 mixture of 22% (w/w).Coating flow process is to terminate with the final coating of 2% hydrogenated palm kernel oil.
After coating, warm cated T-particle of executing cools in envrionment temperature with air in fluidized-bed.In this technological process, fines is removed.
The cated T-particle of executing containing zytase of cooling carries out classification finally by screening, to guarantee that granularity is 250 μm-1180 μm.
Embodiment 5: the measurement of pelleting stability
Granulation test is carried out to the particle of embodiment 1-4 is as described below.Comprise the commercial sample of Ronozyme WX (CT) as benchmark (reference).
Test setting:
By the zytase particle of about 100g and 10kg feed mixing 10min in little level (horizontal) blender.By this premixture and 90kg feed mixing 10min in larger horizontal blender.Feed is imported setter (having the stepwise mixing tank of steam injection) from blender with the speed of about 300kg/ hour.Setter uses steam injection to be heated to by feed 95 DEG C (measuring in outlet).The hold-time of setter is about 30 seconds.Feed is introduced the Simon Hessen press being equipped with the horizontal matrix of 3,0x 35mm (horizontal matrix) from setter, and be compressed to 15 millimeters long particles.After compacting, particle is placed in air-cooler and cools 15 minutes.
Feed forms
73.8% powdery Zea mays
21.7% roasted bean dregs (roasted course soya meal)
4.0% soya-bean oil
0.3%Farmix VLSVRK (from the VITAMIN of Ke-miske Fabrik, Denmark and the commercial mixture of mineral substance)
Water capacity: 12.0%
By determining that relative residual is active by the active Feed Sample activity divided by its corresponding non-pelletizing of the Feed Sample of granulation.
Result:
Sample Residual activity [%] after granulation
Embodiment 1: cellulose-less, uncoated Ronozyme WX particle 95.0
Embodiment 2: Ronozyme WX (CT) particle of plam oil coating 98.0
Embodiment 3: with the Ronozyme WX MG particle of 40% salt coating 85.0
Embodiment 4: the Ronozyme WX (CT) being absorbed into the plam oil coating of cassava core 107.0
Reference: Ronozyme WX (CT) benchmark of plam oil coating 87.0
The above results indicates as drawn a conclusion:
Cellulose-less fiber, uncoated Ronozyme WX has pelleting stability more better than Ronozyme WX (CT) benchmark of plam oil coating.
Cellulose-less fiber, the Ronozyme WX (CT) of plam oil coating has the better pelleting stability of Ronozyme WX (CT) benchmark than plam oil coating.
To absorb in cassava core and with the Ronozyme WX of plam oil coating, to there is pelleting stability more better than Ronozyme WX (CT) benchmark of plam oil coating.
Inertia sodium sulfate carrier higher slice and in fluidized-bed with sodium sulfate layer coating Ronozyme WX compared with Ronozyme WX (CT) benchmark, there is good pelleting stability.

Claims (14)

1. comprise a particle for zytase, described zytase has the aminoacid sequence with the residue 32-225 of SEQ ID NO:1 with at least 90% identity, wherein said particle not containing cellulose fiber.
2. the particle of claim 1, it comprises the uniform mixture of zytase, particle filler and granulation binders.
3. the particle of aforementioned any one claim, it comprises core and coating, and wherein said core comprises zytase, and described coating comprises the hydrophobic substance of the fusing point with more than 35 DEG C.
4. the particle of aforementioned claim, wherein said hydrophobic substance is fat of triglyceride.
5. the particle of aforementioned claim, wherein said coating comprises the inorganic fill agent material of 50-80% further.
6. the particle of claim 1, it comprises:
A. granular core, it is not containing zytase,
B. surround the uniform xylan enzyme layer of described core, it comprises zytase and forms the 5-20% of described core weight, and
C. surround the coating of described xylan enzyme layer, it comprises the salt of at least 60%, and described salt has at 20 DEG C the constant humidity being greater than 81%, forms the 20-100% of described core weight.
7. the particle of claim 1, it comprises
A. core, it can absorb the water of at least 5w/w (weight based on core), and wherein zytase is absorbed in core, and
B. coating, it comprises the hydrophobic substance of the fusing point with more than 35 DEG C, particularly fat of triglyceride.
8. the particle of aforementioned claim, wherein said coating comprises the inorganic fill agent material of 50-80% further.
9. manufacture a method for feed composition, comprise the steps:
A. the zytase particle of feed ingredient with aforementioned any one claim is mixed,
B. composition (a) described in steam treatment, and
C. optionally described composition (b) is carried out granulation.
10. manufacture a method for feed composition, comprise the steps:
A. mixed with zytase particle by feed ingredient, wherein said zytase has the aminoacid sequence having 90% identity with the residue 32-225 of SEQ IDNO:1 at least, and wherein said particle is non-applied layer,
B. composition (a) described in steam treatment, and
C. optionally described composition (b) is carried out granulation.
11. 1 kinds of methods of producing zytase particle; comprise and the mixture and granulation liquid rotary drum that comprise zytase, particle filler and granulation binders are granulated (drum granulating) to form described particle; wherein said zytase has the aminoacid sequence having 90% identity with the residue 32-225 of SEQ ID NO:1 at least, and wherein said granulated mixture not containing cellulose fiber.
The method of 12. aforementioned claims, it comprises further and applies described particle with the mixture of the inorganic fill agent material of the fat or wax and 50-80% comprising the fusing point with 30-100 DEG C.
13. 1 kinds of methods of producing zytase particle, it comprises:
A. carrier is loaded into fluidized-bed,
B. the aqueous solution comprising zytase (with optional tackiness agent) is sprayed on carrier,
It is c. the mixture in fluidized-bed is dry,
D. the aqueous solution of salt is sprayed onto in the material in fluidized-bed, and dry.
14. 1 kinds of methods of producing zytase particle, it comprises:
A. absorbent core contact liq medium is made, described absorbent core can absorb the water of at least 5%w/w (weight based on core), described liquid medium contains the zytase of dissolving and/or discrete form, the amount of the described liquid medium adopted makes products therefrom substantially not occur adjoint cohesion, and wherein said zytase has the aminoacid sequence having 90% identity with the residue 32-225 of SEQ ID NO:1 at least;
B. the volatiles of liquid medium is removed at least in part from described products therefrom, and
C. coating is imposed on described particle.
CN201510141335.XA 2015-02-24 2015-03-27 Xylanase particles Pending CN104774819A (en)

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