CN104774761A - Magnetic bead driving method and apparatus allowing cells to do rectilinear motion in micro-fluidic chip - Google Patents
Magnetic bead driving method and apparatus allowing cells to do rectilinear motion in micro-fluidic chip Download PDFInfo
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- CN104774761A CN104774761A CN201510095216.5A CN201510095216A CN104774761A CN 104774761 A CN104774761 A CN 104774761A CN 201510095216 A CN201510095216 A CN 201510095216A CN 104774761 A CN104774761 A CN 104774761A
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Abstract
The invention discloses a magnetic bead driving method and apparatus allowing cells to do rectilinear motion in a micro-fluidic chip. According to the invention, the cross section of a micro-fluidic channel is an inverted isosceles triangle with a downward vertex; a micro magnetic pole array formed by a plurality of micro magnetic poles and composed of a front column, a middle column and a back column is arranged between a pedestal and the micro-fluidic channel; each micro magnetic pole is composed of a magnetic needle, an electromagnetic iron core and a spiral coil; the center of the top of each electromagnetic iron core is fixedly connected with the lower end of one magnetic needle, and the upper end of each magnetic needle is upwardly inserted into the micro-fluidic channel from the vertex of the inverted isosceles triangle all the upper ends of the magnetic needles inserted into the micro-fluidic channel are located in a same linear extending from left to right; and the on and off states of the spiral coils are optionally controlled via a one-chip microcomputer, so linear arraying, oriented movement and speed regulation of magnetic beads are realized. The magnetic bead driving method and apparatus are not influenced by properties like fluid concentration, a pH value and a dielectric constant, can prevent damage of cells and are beneficial for maintaining activity of cells.
Description
Technical field
The invention belongs to micro-fluidic optical detection system, relate to the device that the motion of detected cell is controlled, be one of key element of decision accuracy of detection to the movement control technology of detected cell, the present invention refers in particular to the magnetic bead drive unit microchannel inner cell of micro-fluidic chip being carried out to translational motion control.
Background technology
Realize highly sensitive optical detection, key is to reduce bias light, particularly exciting light to the impact detecting light path.And exciting light facula area directly depends on stability and the accuracy of detected stream of cells controlled motion.When detected cell is in state homodromous along same linear sequence completely, exciting light hot spot can focus on enough little area to realize hypersensitivity or even single celled detection.At present for the control techniques mainly flow cytometry of cell translational motion, main products is the effect utilizing sheath fluid, the homodromous flow cytometer of order on the axis making the cell be detected be limited in liquid stream.But flow cytometer is expensive, volume is comparatively large, needs professional to operate, not yet complete universal in application departments such as hospital laboratories, more cannot be integrated in micro-fluidic chip.
In micro-fluidic chip, at present the location of cell, seizure, separation and fusion to the main direction of studying of cell manipulation, and it is little for the control method research of cell translational motion, and control method main is at present based on dielectrophoresis technology, as the photoinduction dielectrophoresis technology by photosensitive semiconductor photosensitive generation dielectric power, this technology, by the translational motion of laser facula, can produce to cell the dielectrophoresis force impelling its translational motion continued in theory; Also have mobile dielectrophoresis technology and row ripple dielectrophoresis technology in addition, the two is all lay some strip-like electrodes on microfluidic channel wall, and the former is by optionally to specific electrifying electrodes, to produce pulling force or the thrust of impelling its orientation movement to cell; Although the latter is energized to all electrodes simultaneously, there is specific ac phase difference in adjacent electrode, also can reach the effect impelling cell directional to move.But no matter above-mentioned any technology, all based on dielectrophoresis force, and dielectrophoresis force is inevitably subject to the concentration of sample, the isoparametric restriction of pH value.Simultaneously also there is impact to a certain degree and change to the activity of cell in high-tension environment.This series of problems defines its range of application.Comparatively speaking, magnetophoresis technology is by the interference of above-mentioned factor, and magnetic field force has more intensity high (magnetic field force reaches as high as 1000pN, and dielectrophoresis force is the highest only have 400pN), the advantages such as variable range large (magnetic field force variable range 2-1000pN, dielectrophoresis force is 200-400pN).In addition nanometer super-paramagnetic bead is being applied in immunodetection, there is good specificity.Be convenient to control respectively, enrichment be separated different types of cell.Magnetic bead technology at present in micro-fluidic is mainly used in the separation and fusion that utilize permanent magnet or high-speed double electromagnet cell, does not still have ripe device for the control of cell translational motion.
Summary of the invention
The object of the invention is the magnetic bead driving method and the device that propose control cell translational motion in a kind of micro-fluidic chip, this device accurately can realize the linear arrangement of individual cells in micro-fluidic chip, orientation movement and speed setting, by sample concentration, pH value is isoparametric affects, and the scope of application is broad; The stepless time adjustment to magnetic bead translational motion can be realized by optimal control program.
For achieving the above object, the technical solution that the magnetic bead drive unit of micro-fluidic chip inner cell translational motion of the present invention adopts is: its underpart is base, top is the micro-fluidic runner arranged in left-right direction, the cross section of micro-fluidic runner is summit isosceles triangle down, before being arranged to by multiple micro-magnetic pole between base and micro-fluidic runner, in, micro-array of magnetic dipoles of rear 3 row, each micro-magnetic pole is all by a needle, an electromagnetic core and a spiral winding composition, each electromagnetic core outside is all wound around a spiral winding, the middle, top of each electromagnetic core is fixedly connected with a needle lower end, every root needle upper end is all upwards inserted in micro-fluidic runner from falling the summit of isosceles triangle, insert all needle upper ends in micro-fluidic runner all on same straight line from left to right, micro-chip, parallel port interface module, the multiple output buffer identical with micro-number of magnetic poles and multiple driver module is packaged with in base, micro-chip connects multiple output buffer respectively through parallel port interface module, each output buffer connects a driver module, and each driver module connects a spiral winding.
The technical solution that the magnetic bead driving method of micro-fluidic chip inner cell translational motion of the present invention adopts is: the magnetic bead buffer solution in conjunction with cell entered in micro-fluidic runner, micro-chip triggers driver module work by output buffer, make all spiral winding energisings and make the aobvious magnetic of micro-magnetic pole entirety, magnetize the magnetic bead around all needles and needle upper end, the magnetic bead of needle vicinity is adsorbed, handle damping fluid low frequency by micro-fluidic chip Controlling System back and forth to flow, make magnetic bead realize linear arrangement under the absorption of all needles of linear arrangement.
Make spiral winding all no powers of several the micro-magnetic poles near target travel direction, the spiral winding of all the other micro-magnetic poles is all energized; For the spiral winding of adjacent these two micro-magnetic poles that are not energized and are energized, first connect the spiral winding of no micro-magnetic pole of energising wherein to attract magnetic bead, disconnect the spiral winding of micro-magnetic pole of energising wherein again to discharge magnetic bead, d/d magnetic bead will move on the needle upper end of the new micro-magnetic pole connected towards target travel direction; Afterwards, towards the direction that target travel is contrary, in turn for the spiral winding of every adjacent two micro-magnetic poles, it is all the spiral winding of the micro-magnetic pole first connecting no energising, disconnect the spiral winding of micro-magnetic pole of adjacent energising again, until all magnetic beads are all moved straightly on next needle upper end along target travel direction, so complete first job circulation; Again for the spiral winding of the most adjacent these two the micro-magnetic poles that are not energized and are energized after first job loop ends, second working cycle is completed with the method being similar to first job circulation, until all magnetic beads are all moved to next needle for this reason to target travel direction again to target travel direction, so reciprocation cycle work, until magnetic bead arrives specified location, the orientation movement realizing magnetic bead controls.
To in the orientation movement control process of magnetic bead, by adjusting the energising frequency of the spiral winding of adjacent micro-magnetic pole to control the movement velocity of magnetic bead.
The present invention and prior art ratio, have the following advantages:
1, the present invention is not by the impact of the character such as fluid concentrations, pH value, specific inductivity, is with a wide range of applications.
2, the present invention is for most of damping fluid, as long as magnetic bead type is constant, does not just need to adjust controling parameters, convenient and swift.
3, the present invention is not by the impact of the charging property of cell particle own, and except a small amount of white corpuscle, red corpuscle and magnetotactic cell equal samples are with except faint magnetic, most biological specimen, all without magnetic, therefore avoids the interference in magnetic field in analytic process.
4, of the present invention is non-contact control, does not also have high voltage environment, can prevent the damage of cell, contributes to the activity maintaining cell.
5, in existing control method, magnetic field force has the most wide in range adjustability, and from 2pN to 1000pN, therefore the present invention can realize the wide area stepless regulating of cell speed.
6, nanometer magnetic bead is applied in immunodetection by the present invention, has good specificity, can combine one by one with specific antibody, is convenient to control different types of cell respectively.
Accompanying drawing explanation
Fig. 1 is the structural front view of the magnetic bead drive unit controlling cell translational motion in micro-fluidic chip of the present invention;
Fig. 2 is the left view of Fig. 1;
Fig. 3 is the stereographic map after Fig. 1 removes micro-fluidic runner;
Fig. 4 is micro-magnetic pole and micro-fluidic runner coupling schematic diagram in Fig. 2;
Fig. 5 is pilot circuit schematic diagram of the present invention;
Fig. 6 magnetic bead drive unit of the present invention realizes the workflow diagram of magnetic bead linear arrangement;
Fig. 7 magnetic bead drive unit of the present invention realizes the workflow diagram of magnetic bead orientation movement process;
In figure: 1. micro-fluidic runner; 2. needle; 3. electromagnetic core; 4. spiral winding; 5. bundle conductor; 6. base; 7,8. communication interface.
Embodiment
See Fig. 1,2,3,4, micro-fluidic chip inner cell translational motion of the present invention
'sthe bottom of magnetic bead linear drive apparatus is base 6, and top is micro-fluidic runner 1, and micro-fluidic runner 1 is arranged at left and right directions, and the magnetic bead in conjunction with cell from left to right or from right to left moves in micro-fluidic runner 1.What install between base 6 and micro-fluidic runner 1 is micro-array of magnetic dipoles.
Micro-array of magnetic dipoles is the array architecture of 3 row before, during and after being arranged to by multiple micro-magnetic pole, each micro-magnetic pole is all made up of a needle 2, electromagnetic core 3 and a spiral winding 4, in order to increase magnetic flux, electromagnetic core 3 uses the soft magnetic materials permalloy of high permeability to make, each electromagnetic core 3 outside is all wound around a spiral winding 4, the middle, top of each electromagnetic core 3 is fixedly connected with the lower end of a needle 2, by electromagnetic core 3 and needle 2 secure bond as a whole, the upper end of every root needle 2 all extends upwardly in micro-fluidic runner 1.
Micro-fluidic runner 1 in the cross section of left and right view directions be summit down fall isosceles triangle, when the upper end of needle 2 is stretched in micro-fluidic runner 1, needle 2 is just inserted from the summit of the isosceles triangle of micro-fluidic runner 1, after insertion, is coupled for machinery is accurate with between micro-fluidic runner 1.
The upper end of inserting all needles 2 in micro-fluidic runner 1 is all on same straight line from left to right, and the spacing between all adjacent two needles 2 is identical.In order to ensure the needle 2 on electromagnetic core 3 top of the different positions in arranged in arrays upper end all on the same line, different relative to the position of micro-fluidic runner 1 according to electromagnetic core 3, needle 2 is processed into the shape that vertical-type is different with shaped form two kinds.For before, during and after micro-magnetic poles of 3 row, all needles 2 shape of middle row is all processed into vertical-type, installs straight up; Needle 2 shape of forward and backward two row is all processed into shaped form, upwards installs along shaped form, extends between middle one adjacent two needles 2 arranged.The micro-magnetic pole of middle row linearly arranges from left to right, micro-magnetic pole of forward and backward two row and the micro-magnetic pole cross arrangement of middle row.In the alignment processes of micro-array of magnetic dipoles, by adjusting the angle of micro-magnetic pole, can guarantee that the spacing of all needle 2 upper ends is identical.
Needle 2 is processed by the permalloy of high magnetic permeability, is micron-sized needles, and the diameter of needle 2 upper end is micron order, suitable with the size of Most bacterial and cell.Coordinate micro-fluidic runner 1 to fall the flow channel space design of isosceles triangle and effectively can guarantee adsorbing one to one and ensureing the accurate absorption between needle 2 and magnetic bead to greatest extent of cell and micro-magnetic pole, cell is accurately moved according to the track of straight line.
See Fig. 1 and Fig. 5, encapsulate control module in base 6, base 6 is as the shell of control module.Control module is made up of micro-chip, pilot circuit and driving circuit.Pilot circuit is made up of parallel port interface and multiple output buffers identical with micro-number of magnetic poles, and driving circuit is made up of multiple driver modules identical with micro-number of magnetic poles.Base 6 is provided with two communication interfaces, is communication interface 7 and communication interface 8 respectively, and communication interface 7 is the parallel busses interface with human-machine interface module communication, and communication interface 8 is the RS232 serial bus interface with micro-fluidic Controlling System communication.Micro-chip connects the human-machine interface module of base 6 outside through communication interface 7, connects the micro-fluidic Controlling System of base 6 outside through communication interface 8 respectively.The parallel port interface module of micro-chip also respectively in address bus, data bus, control bus connection control circuit, parallel port interface module connects multiple output buffer respectively, and each output buffer connects a driver module in driving circuit.Driver module connects a spiral winding 4 in micro-magnetic pole, to control spiral winding 4.The upper end of all spiral windings 4 all ties in prick by line concentration and receives and become bundle conductor 5 to be also finally linked into the driving circuit of base 6 inside, and the opening of lower end set by base 6 upper surface of all spiral windings 4 directly accesses the driving circuit of base 6 inside.
The effect of parallel port interface module is the address bus of the quantity expansion micro-chip according to magnetic pole micro-in micro-array of magnetic dipoles, and the signal transmitted by address bus is converted into the chip selection signal of output buffer.The control command of micro-chip to micro-magnetic pole is sent by data bus.The control information that single-chip data bus sends is deposited in the output buffer selected by sheet by parallel port interface module.Trigger driving circuit when output buffer intermediate value is 1, when value is 0, disconnect driving circuit.Control bus is used for exchange of control information between micro-chip and parallel port interface module, as reset signal when synchronous clock, interface circuit chip selection signal, startup etc.After output buffer is set, the relay conducting in driving circuit in corresponding driver module, has electric current to pass through in the spiral winding 4 of corresponding micro-magnetic pole, makes to produce magnetic line of force in corresponding electromagnetic core 3, is magnetized, makes micro-magnetic pole show magnetic.
When the present invention works, the power on/off of the spiral winding 4 optionally controlled on micro-array of magnetic dipoles by micro-chip realizes linear arrangement to magnetic bead, orientation movement and speed setting.Specific implementation process is as follows:
See Fig. 1-5 and Fig. 6, realization to the linear arrangement process of magnetic bead is: the spiral winding 4 of all micro-magnetic poles is all in off-position, magnetic bead buffer solution in conjunction with cell is entered in micro-fluidic runner 1 under Micropump drives, now, the magnetic bead NATURAL DISTRIBUTION in micro-fluidic runner 1.Afterwards, micro-chip triggers the rly. conducting in driving circuit by output buffer, driver module works, all spiral windings 4 of micro-magnetic pole are energized and make the aobvious magnetic of micro-magnetic pole entirety, thus the magnetic bead magnetized rapidly around all needles 2 and needle 2 upper end, the magnetic bead carrying cell of needle 2 vicinity can be attracted to this needle 2 upper end, magnetic bead now in damping fluid is in three kinds of states: the needle 2 summit place that part magnetic bead is contiguous because the action of a magnetic field attracted to, and other a part of magnetic bead does not have needle 2 due to neighbouring, or due to neighbouring needle 2 summit having had corresponding magnetic bead to adsorb, free movement can be in or be enriched in the state of periphery of contiguous needle 2.Now, handle damping fluid microfluid low frequency by micro-fluidic chip Controlling System back and forth to flow, in the reciprocating process of damping fluid microfluid, be adsorbed on the effect of the magnetic bead on needle 2 due to magnetic field force, motion can not be produced, and free movement or the magnetic bead that is enriched in periphery, contiguous needle 2 upper end due to institute magnetic field force induced very little, be not enough to the reactive force overcoming fluid, fluid will be followed move reciprocatingly, in the process, contact is not yet adsorbed the needle 2 of magnetic bead and is produced absorption by magnetic bead, realizes bi-directional matching attachment.Magnetic bead achieves linear arrangement under the absorption of all needles 2 of linear arrangement.When spiral winding 4 power-off of micro-magnetic pole, corresponding electromagnetic core 3 loses magnetism, and due to the characteristic that magnetic bead magnetic hysteresis is minimum, magnetic bead is in free state by losing magnetism instantaneously.In the present invention, the length size that is equal and micron-sized cell that the size of micro-fluidic runner 1 of isosceles triangle, micron-sized needle 2 upper end size and micro-needle 2 embed micro-fluidic runner 1 of falling matches, and improves the accuracy of bi-directional matching attachment to greatest extent.
After the micro-magnetic pole of all energisings has all adsorbed, after magnetic bead linear arrangement completes, when needing cleaning runner, controlled the damping fluid got rid of containing free magnetic bead and cell by microfluidic system, in micro-fluidic runner 1, introduce the wash buffer runner not having magnetic bead and bacterium.Specifically: after all spiral windings 4 are energized 120 seconds, micro-chip sends runner cleaning triggering signal to micro-fluidic Controlling System, under the effect of Micropump, damping fluid is left micro-fluidic runner 1 by driving, injects the new buffer solution for cleaning runner without magnetic bead and cell simultaneously.Due to the effect of magnetic force, be attracted to cell on needle 2 and magnetic bead combination still keeps original position, and will be rinsed into waste liquid pool by the free cell that adsorbs and magnetic bead combination.No longer free magnetic bead and cell in new damping fluid, to guarantee in the process that anaphase movement controls not from the magnetic bead of free state and the interference of cell.
See Fig. 1-5 and Fig. 7, realization to the orientation movement process control of magnetic bead is: utilize linear electric motors principle, by controlling cut-offfing in order of the spiral winding 4 of adjacent micro-magnetic pole, make the magnetic bead orientation movement of attached cell, realize magnetic bead and control to the translational motion of left or right two target directions under the driving of micro-magnetic pole.The energising order of its direction of motion spiral winding 4 of the most adjacent two micro-magnetic poles under depending on micro-chip control.Make spiral winding 4 all no powers of several the micro-magnetic poles near target travel direction, and the spiral winding 4 of all the other micro-magnetic poles is all energized, magnetic bead is not adsorbed in needle 2 upper end of these cold micro-magnetic poles, and magnetic bead has been adsorbed in needle 2 upper end of micro-magnetic pole of energising; Then, for the spiral winding 4 of adjacent these two micro-magnetic poles that are not energized and are energized, first connect the spiral winding 4 of no micro-magnetic pole of energising wherein to attract magnetic bead, disconnect the spiral winding 4 of micro-magnetic pole of energising wherein again to discharge magnetic bead, d/d magnetic bead will move on needle 2 upper end of the new micro-magnetic pole connected towards target travel direction; Afterwards, towards the direction that target travel is contrary, in turn for the spiral winding 4 of every adjacent two micro-magnetic poles, it is all the spiral winding 4 of the micro-magnetic pole first connecting no energising, disconnect the spiral winding 4 of micro-magnetic pole of adjacent energising again, until all magnetic beads are all moved straightly on next needle 2 upper end along target travel direction, so complete first job circulation.After first job loop ends, again for the spiral winding 4 of the most adjacent these two the micro-magnetic poles that are not energized and are energized after first job loop ends, the energising order controlling the spiral winding 4 of adjacent two micro-magnetic poles completes second working cycle, until all magnetic beads are all moved to next needle 2 for this reason to target travel direction again to target travel direction with the method being similar to first job circulation.So reciprocation cycle work, until magnetic bead arrives specified location.
Magnetic bead orientation movement is supported in order to there be fully many micro-magnetic pole allowances of not adsorbing magnetic bead, best practice adopts spiral winding 4 no power on M micro-magnetic pole near moving target side, 5≤M≤N, N is micro-magnetic pole sum, and adopts the spiral winding 4 on other N-M away from moving target direction micro-magnetic poles to be energized.Such as: sequentially number from the micro-magnetic pole near moving target, now the 1st is in off-position to the spiral winding 4 of M micro-magnetic pole, M+1 and above until the spiral winding 4 of the N number of micro-magnetic pole is in switch-on regime.When orientation movement starts, the spiral winding 4 of M the micro-magnetic pole be not energized first is made to be energized, corresponding M needle 2 just produces the suction to around magnetic bead, micro-chip starts timing register time delay T timing second subsequently, T is the minimum delay time of micro-chip, by spiral winding 4 power-off of the M+1 of correspondence micro-magnetic pole after timing terminates, M+1 micro-magnetic pole is lost magnetism, magnetic bead corresponding on its needle 2 upper end is subject to the attraction of the needle 2 of adjacent M micro-magnetic pole and moves to extreme direction on M magnetic pole 2, thus achieve the transmission of a cell magnetic bead combination between adjacent two magnetic poles 2.After T time delay second, in turn M+1 micro-magnetic pole is energized, to M+2 micro-magnetic pole power-off, carries out similar operation; Again after T time delay second, in turn to M+2 micro-magnetic pole energising, each and every one micro-magnetic pole power-off of M+3, operate accordingly, the rest may be inferred, until complete the operation of the micro-magnetic pole of all energisings.Now all magnetic beads move on next needle 2 upper end to target travel direction, complete a working cycle, after this working cycle terminates, the 1st micro-magnetic pole is all in off-position to M-1 micro-magnetic pole, and M is individual and above micro-magnetic pole is all in switch-on regime.According to the control law that a upper working cycle is identical, individual for adjacent two micro-magnetic poles with the M of the M-1 be not energized a micro-magnetic pole and energising, start to be energized the control of order, complete the working cycle identical with a upper working cycle, often complete a working cycle, all magnetic beads all move to next needle 2 to target travel direction.And so forth, until arrive specified location.
To in the orientation movement control process of magnetic bead, by adjusting the energising frequency of the spiral winding 4 of adjacent micro-magnetic pole, the movement velocity of magnetic bead just can be controlled.Specifically: by the speed regulating the delay value T of delay counter to regulate magnetic bead to move, T is larger, represents the control interval longer, and magnetic bead movement velocity is slower.Otherwise T is less, the control interval is shorter, and movement velocity is faster, to realize the speed setting to magnetic bead.When the increment of T or enough hour of decrement, be just considered as stepless time adjustment.
Claims (8)
1. the magnetic bead drive unit of a micro-fluidic chip inner cell translational motion, its underpart is base (6), top is the micro-fluidic runner (1) arranged in left-right direction, it is characterized in that: the cross section of micro-fluidic runner () is summit isosceles triangle down, before being arranged to by multiple micro-magnetic pole between base (6) and micro-fluidic runner (1), in, micro-array of magnetic dipoles of rear 3 row, each micro-magnetic pole is all by a needle (2), an electromagnetic core (3) and spiral winding (4) composition, each electromagnetic core (3) outside is all wound around a spiral winding (4), the middle, top of each electromagnetic core (3) is fixedly connected with needle (2) lower end, every root needle (2) upper end is all upwards inserted in micro-fluidic runner (1) from falling the summit of isosceles triangle, insert all needles (2) upper end in micro-fluidic runner (1) all on same straight line from left to right, micro-chip, parallel port interface module, the multiple output buffer identical with micro-number of magnetic poles and multiple driver module is packaged with in base, micro-chip connects multiple output buffer respectively through parallel port interface module, each output buffer connects a driver module, and each driver module connects a spiral winding (4).
2. magnetic bead drive unit according to claim 1, is characterized in that: all needles (2) of middle row are all vertical-types, and left-to-right linearly arranges; The needle (2) of forward and backward two row is all shaped forms, and between adjacent two needles (2) of middle row.
3. magnetic bead drive unit according to claim 1, is characterized in that: micro-magnetic poles of forward and backward two row and the micro-magnetic pole cross arrangement of middle row, the spacing between all adjacent two needles (2) is identical.
4. the driving method of a magnetic bead drive unit as claimed in claim 1, it is characterized in that: the magnetic bead buffer solution in conjunction with cell is entered in micro-fluidic runner (1), micro-chip triggers driver module work by output buffer, make all spiral winding (4) energisings and make the aobvious magnetic of micro-magnetic pole entirety, magnetize the magnetic bead around all needles (2) and needle (2) upper end, the magnetic bead of needle (2) vicinity is adsorbed, handle damping fluid low frequency by micro-fluidic chip Controlling System back and forth to flow, magnetic bead is made to realize linear arrangement under the absorption of all needles (2) of linear arrangement.
5. magnetic bead driving method according to claim 4, it is characterized in that: when needing cleaning runner after magnetic bead linear arrangement completes, controlled by microfluidic system in micro-fluidic runner (1), introduce the damping fluid not having magnetic bead and cell, be attracted to cell on needle (2) and magnetic bead combination still keeps original position, and be not rinsed by the free cell that adsorbs and magnetic bead combination.
6. magnetic bead driving method according to claim 4, is characterized in that: spiral winding (4) all no powers making several the micro-magnetic poles near target travel direction, and the spiral winding (4) of all the other micro-magnetic poles is all energized; For the spiral winding (4) of adjacent these two micro-magnetic poles that are not energized and are energized, first connect the spiral winding (4) of no micro-magnetic pole of energising wherein to attract magnetic bead, disconnect the spiral winding (4) of micro-magnetic pole of energising wherein again to discharge magnetic bead, d/d magnetic bead will move on needle (2) upper end of the new micro-magnetic pole connected towards target travel direction; Afterwards, towards the direction that target travel is contrary, in turn for the spiral winding (4) of every adjacent two micro-magnetic poles, it is all the spiral winding (4) of the micro-magnetic pole first connecting no energising, disconnect the spiral winding (4) of micro-magnetic pole of adjacent energising again, until all magnetic beads are all moved straightly on next needle (2) upper end along target travel direction, so complete first job circulation; Again for the spiral winding (4) of the most adjacent these two the micro-magnetic poles that are not energized and are energized after first job loop ends, second working cycle is completed with the method being similar to first job circulation, until all magnetic beads are all moved to next needle (2) for this reason to target travel direction again to target travel direction, so reciprocation cycle work, until magnetic bead arrives specified location, the orientation movement realizing magnetic bead controls.
7. magnetic bead driving method according to claim 6, is characterized in that: in the orientation movement control process of magnetic bead, by adjusting the energising frequency of the spiral winding (4) of adjacent micro-magnetic pole to control the movement velocity of magnetic bead.
8. magnetic bead driving method according to claim 7, is characterized in that: by the speed regulating the delay value of micro-chip delay counter to control magnetic bead motion, delay value is larger, and magnetic bead movement velocity is slower, and delay value is less, and magnetic bead movement velocity is faster.
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| CN107043700B (en) * | 2017-06-08 | 2023-11-24 | 杭州遂真生物技术有限公司 | Control method of magnetic control system for gene detection |
| CN107621486A (en) * | 2017-08-09 | 2018-01-23 | 江苏大学 | The program control layout inoculation method of cell and device of Cell-oriented impedance detection research |
| CN107621486B (en) * | 2017-08-09 | 2019-12-03 | 江苏大学 | The program-controlled layout inoculation method of the cell of Cell-oriented impedance detection research and device |
| CN107764994A (en) * | 2017-11-21 | 2018-03-06 | 南京罗岛纳米科技有限公司 | The device spread using magnetic bead control DNA in the sequencing experiment of solid nano hole |
| CN112433058A (en) * | 2020-11-20 | 2021-03-02 | 迈克医疗电子有限公司 | Magnetic bead detection control method and device, terminal equipment and medium |
| CN112433058B (en) * | 2020-11-20 | 2023-09-15 | 迈克医疗电子有限公司 | Method, device, terminal equipment and medium for detecting and controlling magnetic beads |
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