CN104770327A - Hatching method of quasipaa spinosa frogspawn - Google Patents

Hatching method of quasipaa spinosa frogspawn Download PDF

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Publication number
CN104770327A
CN104770327A CN201510125493.6A CN201510125493A CN104770327A CN 104770327 A CN104770327 A CN 104770327A CN 201510125493 A CN201510125493 A CN 201510125493A CN 104770327 A CN104770327 A CN 104770327A
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hatching
frame
side frame
eaves
tadpole
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CN104770327B (en
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余智杰
李志元
章海鑫
陶志英
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Bright Moon Mountain Jiangxi Stone Frog Cultivation Ltd Co
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Bright Moon Mountain Jiangxi Stone Frog Cultivation Ltd Co
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Abstract

The invention belongs to the field of aquaculture, and particularly relates to a hatching method of quasipaa spinosa frogspawn. The method comprises the following steps of preparation of hatching ambient conditions, use of a hatching frame, hatching operation and cultivation of tadpole out of a membrane, wherein the hatching ambient conditions include hatching pond conditions and hatching environment, and the hatching operation comprises three-layer filtration of hatching water. The hatching ambient conditions, the use of the hatching frame, the hatching operation and the cultivation of the tadpole out of the membrane are optimized, and the illumination intensity of a hatching pond, the dissolved oxygen and pH of water in the hatching pond, the specific form of the used hatching frame, the hatching density, specific operations of the three-layer filtration of the hatching water and the cultivation of the tadpole out of the membrane are further optimized, so that the tadpole hatching density can reach 2000 tadpoles per square meter, and the tadpole hatching rate is increased, so that hatched small tadpoles are strong, and a good foundation is provided for the subsequent tadpole cultivation.

Description

A kind of hatching m of Ranaspinosa David frog's egg
Technical field
The invention belongs to aquaculture field, be specifically related to a kind of hatching m of Ranaspinosa David frog's egg.
Background technology
Ranaspinosa David, has another name called the stone frog, chukar, the hole frog, stone squama, stone freezes, belong to amphibian animal ranid, be mainly distributed in south China mountain area.More than 85% of its stomach inclusion is the insect of harm tree growth; Body is large and sturdy, Fresh & Tender in Texture, nutritious, and nourishing effects is subject to the favor of people.Because people are to the increase day by day of Ranaspinosa David demand and the day by day deficient of wild resource, the market price of Ranaspinosa David also rises thereupon, therefore, carries out propagating artificially of Ranaspinosa David and has significant society, ecology and economic benefit.
The incubation rate of Ranaspinosa David is not only relevant with the quality of frog's egg itself, also manages close relationship with spawn incubation.Good hatching management not only directly improves spawn incubation rate, can also reduce the damage of frog's egg and tadpole seedling, provide the tadpole seedling of physique stalwartness.
" brief talking the artificial incubation technique main points of Ranaspinosa David " describes the artificial incubation technique main points of Ranaspinosa David, comprise frog's egg collection, hatching density, incubation equipment, hatching management etc., and specifically discuss following content: " hatching density of Ranaspinosa David ovum is closely related with hatching survival rate.Adopt cement pit hatching frog's egg, suitable hatching density is 5000 ~ 6000/m 2.The depth of water remains on 30 ~ 50cm.Incubation equipment except hatchery, also available incubation net cage, hatching frame, plastic basin or other containers etc.And the points for attention in hatching process, as removed dead ovum etc. ".
But the shortcoming of above-mentioned Ranaspinosa David artificial incubation method is obvious, and operational observations is inconvenient; Hatching density is up to 10000/m 2, with ovum footpath 1.5-2.0cm after the water suction of Ranaspinosa David ovum, density is too large, easily piles up, causes anoxic, affect incubation rate; Manage numerous and diverse, operation is many, need regularly remove dead ovum, and labour intensity is large, also large to the mechanically actuated of frog's egg; Fuzzyyer to environment of hatching index request, quantizating index is not had to intensity of illumination etc., causes managerial uncertainty; Be not strict with Spawning water, likely making harmful organisms enter affects spawn incubation; And prophase management is not had to tadpole, do not reach the object of cultivating healthy and strong tadpole seedling, tadpole survival rate reduces, thus constrains the large-scale production of Ranaspinosa David.
Summary of the invention
For this reason; to be solved by this invention be the hatching density of existing Ranaspinosa David excessive, easily pile up; incubation condition is harsh, it is numerous and diverse to manage, operational observations is inconvenient and make the incubation rate of tadpole and early stage cultivating survival rate reduce thus constrain the technical problem of the large-scale production of Ranaspinosa David, thus propose that the environmental condition indexs such as a kind of incubation condition is simple and easy to do, illumination specifically quantize, water quality requirement is strict, the incubation rate of tadpole and early stage cultivating survival rate high and the hatching m of the Ranaspinosa David frog's egg of the tadpole seedling physique stalwartness of hatching.
For solving the problems of the technologies described above, the present invention is achieved through the following technical solutions:
The hatching m of a kind of Ranaspinosa David frog's egg of the present invention, the method comprises the steps: environment of hatching condition; The use of hatching frame; Hatching operation; Tadpole after membrane is cultivated;
Wherein, described environment of hatching condition, comprises the condition of hatchery and the environment of hatching; Described hatching operation comprises carries out three layer filtration to Spawning water.
The above-mentioned hatching m of the present invention, the condition of described hatchery is: select area 6m 2, the dark 60cm in pond cement hatchery, construct at the bottom of pond and wall by masonry, cement, mortar whitewash, and finally use cement mortar floating, require at the bottom of pond smooth, do not seep water; Enter discharge outlet diagonal angle to arrange, delivery port to build at the bottom of pond one jiao in, if regulate water level to begin to crop up, water inlet is located at above the water surface, keeps whole pond water body to exchange, creates miniflow water environment;
The environment of described hatching is: build sunshade, rain shade above described hatchery, keep described hatchery intensity of illumination 2000 ~ 3000lux, rainwater is avoided to flow directly in described hatchery, the water level mean depth of described hatchery remains on 30 ~ 40cm, water quality dissolved oxygen is not less than 5mg/L, and water quality pH is 6.5 ~ 8.
The above-mentioned hatching m of the present invention, the use of described hatching frame is: each described hatchery places described hatching frame 10-13, and tiling is put in the water surface, and each described hatching frame puts 1000, fertilized egg, and averag density is 2000/m 2, make described fertilized egg keep below the water surface, water inlet can not flow directly in frame, will gently flow into described hatchery.
The above-mentioned hatching m of the present invention, the use of described hatching frame hatches frame for using anti-escaping, and described anti-hatching frame of escaping comprises frame body, and is arranged on the screen cloth of bottom of described frame body; Described frame body is by side frame around being formed, and described side frame top is provided with perpendicular to described side frame and the frame eaves inwardly detained, and described screen cloth is 10-25 order screen cloth.
The above-mentioned hatching m of the present invention, described frame body by first side frame, second side frame, the 3rd side frame, four side frame around being formed, described frame eaves by the first frame eaves, the second frame eaves, the 3rd frame eaves, the 4th frame eaves around being formed, described first side frame and described second side frame are oppositely arranged, described 3rd side frame, described four side frame respectively with described first side frame, described second side frame is adjacent and be oppositely arranged; Described first frame eaves, described second frame eaves, described 3rd frame eaves, described 4th frame eaves are adjacent with described first side frame, described second side frame, described 3rd side frame, described four side frame respectively and vertically arrange, described first frame eaves and described second frame eaves are oppositely arranged, described 3rd frame eaves, described 4th frame eaves respectively with described first frame eaves, described second frame eaves is adjacent and be oppositely arranged.
The above-mentioned hatching m of the present invention, the distance of described first frame eaves and described first side frame and the length ratio of described first side frame are 2 ~ 3:76, the distance of described first frame eaves and described first side frame and the length ratio of described 3rd side frame are 2 ~ 3:50, and the distance of described first frame eaves and described first side frame is 2 ~ 3:4 with the ratio of the height of described first side frame.
The above-mentioned hatching m of the present invention, described to carry out three layer filtration to Spawning water as follows: described Spawning water requires that water quality is pure and fresh, pollution-free, and dissolved oxygen is sufficient; Water inlet will through 3 layers of filtration, and ground floor adopts woven wire filter, prevents from entering at dirts such as the large-scale harmful organisms such as the frog, snake and leaves; The second layer adopts 5 ~ 20 order screen cloths to filter, and stops the harmful animal such as small fish and shrimp, tadpole to enter; Third layer adopts 60-70 order screen cloth to filter, in order to control cladocera, copepoda, in order to avoid its harm frog's egg and squab small tadpole.
The above-mentioned hatching m of the present invention, described hatching operation also comprises:
The output time phase difference ovum of 1-2 days, can be used as and hatch with a collection of being placed in same described hatchery, and the output time can be placed at ovum on the same day samely describedly anti-escapes in hatching frame;
Diligent inspection between the incubation period, flood, slime water can not enter pond, prevent pieces of an egg from piling up overlapping, Timing measurement water temperature, temperature, observe water quality and embryonic development situation, make a record, and pinpoint the problems and process in time.
The above-mentioned hatching m of the present invention, the tadpole after described membrane is cultivated and is: just hatching small tadpole yolk out does not also absorb completely, and health is tiny, also should not shift out described anti-escaping and hatch frame, needs to support about 10 days temporarily in hatching frame described anti-escaping; Firm membrane 3 days tadpoles do not ingest, by absorbing yolk growth; The complete rear tadpole of absorption of yolk at its ingests egg membrane, does not need to throw something and feed; After 7 days, throw something and feed 1 yolk that boils of each ten thousand tail supplements the nutrients, training ingestion ability; When tadpole grows to 2-3cm, tadpole has certain Burden-Swimming Ability of KM, independently can ingest and just can put into the cultivation of tadpole pond.
Technique scheme of the present invention has the following advantages compared to existing technology:
(1) hatching m of Ranaspinosa David frog's egg of the present invention, by optimizing environment of hatching condition, hatching the use of frame, hatching the tadpole cultivation after operation and membrane, tadpole after the dissolved oxygen of the water quality in the intensity of illumination of hatchery described in one-step optimization, described hatchery of going forward side by side, pH, the concrete form of hatching frame used, hatching density, the concrete operations described Spawning water being carried out to three layer filtration and described membrane is cultivated, improve the incubation rate of tadpole, make the tadpole seedling physique of hatching healthy and strong, cultivate for next step tadpole and lay a good foundation.
(2) hatching m of Ranaspinosa David frog's egg of the present invention, incubation condition is simple and easy to do, and operation is few, when only having pieces of an egg to pile journey, just need be separated gently.
(3) hatching m of Ranaspinosa David frog's egg of the present invention, the small tadpole of just having hatched out is supported about 10 days in hatching frame temporarily described anti-escaping, be conducive to training firm membrane tadpole initial feeding, after cultivating into healthy and strong tadpole, just descend pond, improve the survival rate of tadpole cultivation in early stage.
(4) hatching m of Ranaspinosa David frog's egg of the present invention, the hatching frame used can either meet frog's egg and tadpole to the demand of oxygen, also the escape of the drift of frog's egg and the small tadpole of just hatching can effectively be prevented, thus the quantity of the frog's egg that can calculate simply, easily in hatching apparatus or tadpole, be conducive to the hatching management of frog's egg and the aquaculture management of tadpole.
Accompanying drawing explanation
In order to make content of the present invention be more likely to be clearly understood, below according to a particular embodiment of the invention and by reference to the accompanying drawings, the present invention is further detailed explanation, wherein
Fig. 1 is the vertical view that the Ranaspinosa David frog's egg of the embodiment of the present invention 1 is prevented escaping to hatch frame;
Fig. 2 is the positive sectional view that the Ranaspinosa David frog's egg of the embodiment of the present invention 1 is prevented escaping to hatch frame;
Fig. 3 is the right section figure that the Ranaspinosa David frog's egg of the embodiment of the present invention 1 is prevented escaping to hatch frame;
In figure, Reference numeral is expressed as: 31-first side frame, 32-second side frame, 33-the 3rd side frame, 34-four side frame, 51-first frame eaves, 52-second frame eaves, 53-the 3rd frame eaves, 54 the 4th frame eaves, the distance of the first frame eaves and described first side frame described in A-, the length of first side frame 31 described in B-, the length of the 3rd side frame 33 described in C-, the height of first side frame 31 described in D-.
Embodiment
Embodiment 1
Anti-the escaping of the present embodiment Ranaspinosa David frog's egg hatches frame, as shown in Figure 1, Figure 2 and Figure 3, comprises frame body, and is arranged on the screen cloth of bottom of described frame body; Described frame body is by side frame around being formed, and described side frame top is provided with perpendicular to described side frame and the frame eaves inwardly detained, and described screen cloth is 10-25 order screen cloth.Described frame body by first side frame 31, second side frame 32, the 3rd side frame 33, four side frame 34 around being formed, described frame eaves by the first frame eaves 51, second frame eaves 52, the 3rd frame eaves 53, the 4th frame eaves 54 around being formed, described first side frame 31 and described second side frame 32 are oppositely arranged, described 3rd side frame 33, described four side frame 34 respectively with described first side frame 31, described second side frame 32 is adjacent and be oppositely arranged; Described first frame eaves 51, described second frame eaves 52, described 3rd frame eaves 53, described 4th frame eaves 54 are adjacent with described first side frame 31, described second side frame 32, described 3rd side frame 33, described four side frame 34 respectively and vertically arrange, described first frame eaves 51 is oppositely arranged with described second frame eaves 52, described 3rd frame eaves 53, described 4th frame eaves 54 respectively with described first frame eaves 51, described second frame eaves 52 is adjacent and be oppositely arranged.
Preferably, the distance A of described first frame eaves 51 and described first side frame 31 is 2 ~ 3:76 with the ratio of the length B of described first side frame 31, the distance A of described first frame eaves 51 and described first side frame 31 is 2 ~ 3:50 with the ratio of the length C of described 3rd side frame 33, and the distance A of described first frame eaves 51 and described first side frame 31 is 2 ~ 3:4 with the ratio of the height D of described first side frame 31.
Further preferably, the described distance A of the present embodiment Ranaspinosa David spawn incubation device is 2 ~ 3cm, and accordingly, the length B of described first side frame 31 is 76cm, and the length C of described 3rd side frame 33 is 50cm, and the height D of described first side frame 31 is 4cm.The wooden frame of described first side frame 31, described second side frame 32, described 3rd side frame 33, described four side frame to be thickness be 4cm.Described first frame eaves 51, described second frame eaves 52, described 3rd frame eaves 53, described 4th frame eaves 54 for thickness be the wooden frame of 1cm.
Above-mentioned anti-hatching frame of escaping can either meet frog's egg and tadpole to the demand of oxygen, also the escape of the drift of frog's egg and the small tadpole of just hatching can effectively be prevented, thus the quantity of the frog's egg that can calculate simply, easily in hatching apparatus or tadpole, be conducive to the hatching management of frog's egg and the aquaculture management of tadpole.
Embodiment 2
The hatching m of a kind of Ranaspinosa David frog's egg of the present embodiment, the method comprises the steps: environment of hatching condition; The use of hatching frame; Hatching operation; Tadpole after membrane is cultivated.
The present embodiment uses anti-the escaping of the Ranaspinosa David frog's egg in embodiment 1 to hatch frame.
Environment of hatching condition is: the condition of (1) hatchery is: select area 6m 2, the dark 60cm in pond cement hatchery, construct at the bottom of pond and wall by masonry, cement, mortar whitewash, and finally use cement mortar floating, require at the bottom of pond smooth, do not seep water; Enter discharge outlet diagonal angle to arrange, delivery port to build at the bottom of pond one jiao in, if regulate water level to begin to crop up, water inlet is located at above the water surface, keeps whole pond water body to exchange, creates miniflow water environment; (2) environment of hatching is: build sunshade, rain shade above described hatchery, keep described hatchery intensity of illumination 2000 ~ 3000lux, rainwater is avoided to flow directly in described hatchery, the water level mean depth of described hatchery remains on 30 ~ 40cm, water quality dissolved oxygen is not less than 5mg/L, and water quality pH is 6.5 ~ 8.
The use of hatching frame is: described hatching frame 10 ~ 13 is placed in each described hatchery, and tiling is put in the water surface, and each described hatching frame puts 1000, fertilized egg, and averag density is 2000/m 2, make described fertilized egg keep below the water surface, water inlet can not flow directly in frame, will gently flow into described hatchery.
Hatching is operating as: (1) output time phase difference ovum of 1-2 days, can be used as and hatches with a collection of being placed in same described hatchery, and the output time can be placed at ovum on the same day samely describedly anti-escapes in hatching frame; (2) described Spawning water requires that water quality is pure and fresh, pollution-free, and dissolved oxygen is sufficient; Water inlet will through 3 layers of filtration, and ground floor adopts woven wire filter, prevents from entering at dirts such as the large-scale harmful organisms such as the frog, snake and leaves; The second layer adopts 5 ~ 20 order screen cloths to filter, and stops the harmful animal such as small fish and shrimp, tadpole to enter; Third layer adopts 60-70 order screen cloth to filter, in order to control cladocera, copepoda, in order to avoid its harm frog's egg and squab small tadpole; (3) diligent inspection between the incubation period, flood, slime water can not enter pond, prevent pieces of an egg from piling up overlapping, Timing measurement water temperature, temperature, observe water quality and embryonic development situation, make a record, and pinpoint the problems and process in time.
Tadpole after membrane is cultivated: just hatching small tadpole yolk out does not also absorb completely, and health is tiny, also should not shift out described anti-escaping and hatch frame, needs to support about 10 days temporarily in hatching frame described anti-escaping; Firm membrane 3 days tadpoles do not ingest, by absorbing yolk growth; The complete rear tadpole of absorption of yolk at its ingests egg membrane, does not need to throw something and feed; After 7 days, throw something and feed 1 yolk that boils of each ten thousand tail supplements the nutrients, training ingestion ability; When tadpole grows to 2-3cm, tadpole has certain Burden-Swimming Ability of KM, independently can ingest and just can put into the cultivation of tadpole pond.
Above-mentioned hatching m, by optimizing environment of hatching condition, hatching the use of frame, hatching the tadpole cultivation after operation and membrane, tadpole after the dissolved oxygen of the water quality in the intensity of illumination of hatchery described in one-step optimization, described hatchery of going forward side by side, pH, the concrete form of hatching frame used, hatching density, the concrete operations described Spawning water being carried out to three layer filtration and described membrane is cultivated, and improve the incubation rate of tadpole, make the tadpole seedling physique of hatching healthy and strong, cultivate for next step tadpole and lay a good foundation.
Obviously, above-described embodiment is only for clearly example being described, and the restriction not to embodiment.For those of ordinary skill in the field, can also make other changes in different forms on the basis of the above description.Here exhaustive without the need to also giving all embodiments.And thus the apparent change of extending out or variation be still among the protection domain of the invention.

Claims (9)

1. a hatching m for Ranaspinosa David frog's egg, is characterized in that, the method comprises the steps: environment of hatching condition; The use of hatching frame; Hatching operation; Tadpole after membrane is cultivated;
Wherein, described environment of hatching condition, comprises the condition of hatchery and the environment of hatching; Described hatching operation comprises carries out three layer filtration to Spawning water.
2. hatching m according to claim 1, is characterized in that,
The condition of described hatchery is: select area 6m 2, the dark 60cm in pond cement hatchery, construct at the bottom of pond and wall by masonry, cement, mortar whitewash, and finally use cement mortar floating, require at the bottom of pond smooth, do not seep water; Enter discharge outlet diagonal angle to arrange, delivery port to build at the bottom of pond one jiao in, if regulate water level to begin to crop up, water inlet is located at above the water surface, keeps whole pond water body to exchange, creates miniflow water environment;
The environment of described hatching is: build sunshade, rain shade above described hatchery, keep described hatchery intensity of illumination 2000 ~ 3000lux, rainwater is avoided to flow directly in described hatchery, the water level mean depth of described hatchery remains on 30 ~ 40cm, water quality dissolved oxygen is not less than 5mg/L, and water quality pH is 6.5 ~ 8.
3. hatching m according to claim 1 and 2, is characterized in that, the use of described hatching frame is:
Described hatching frame 10 ~ 13 is placed in each described hatchery, and tiling is put in the water surface, and each described hatching frame puts 1000, fertilized egg, and averag density is 2000/m 2, make described fertilized egg keep below the water surface, water inlet can not flow directly in frame, will gently flow into described hatchery.
4. according to the arbitrary described hatching m of claim 1-3, it is characterized in that, the use of described hatching frame hatches frame for using anti-escaping, and described anti-hatching frame of escaping comprises frame body, and is arranged on the screen cloth of bottom of described frame body; Described frame body is by side frame around being formed, and described side frame top is provided with perpendicular to described side frame and the frame eaves inwardly detained, and described screen cloth is 10-25 order screen cloth.
5. hatching m according to claim 4, it is characterized in that, described frame body is by first side frame (31), second side frame (32), 3rd side frame (33), four side frame (34) is around formation, described frame eaves is by the first frame eaves (51), second frame eaves (52), 3rd frame eaves (53), 4th frame eaves (54) is around formation, described first side frame (31) and described second side frame (32) are oppositely arranged, described 3rd side frame (33), described four side frame (34) respectively with described first side frame (31), described second side frame (32) is adjacent and be oppositely arranged, described first frame eaves (51), described second frame eaves (52), described 3rd frame eaves (53), described 4th frame eaves (54) respectively with described first side frame (31), described second side frame (32), described 3rd side frame (33), described four side frame (34) is adjacent and vertically arrange, described first frame eaves (51) and described second frame eaves (52) are oppositely arranged, described 3rd frame eaves (53), described 4th frame eaves (54) respectively with described first frame eaves (51), described second frame eaves (52) is adjacent and be oppositely arranged.
6. hatching m according to claim 5, it is characterized in that, described first frame eaves (51) is 2 ~ 3:76 with the distance (A) of described first side frame (31) and the ratio of the length (B) of described first side frame (31), described first frame eaves (51) is 2 ~ 3:50 with the distance (A) of described first side frame (31) and the ratio of the length (C) of described 3rd side frame (33), described first frame eaves (51) is 2 ~ 3:4 with the distance (A) of described first side frame (31) and the ratio of the height (D) of described first side frame (31).
7. according to the arbitrary described hatching m of claim 1-6, it is characterized in that, described to carry out three layer filtration to Spawning water as follows: described Spawning water requires that water quality is pure and fresh, pollution-free, and dissolved oxygen is sufficient; Water inlet will through 3 layers of filtration, and ground floor adopts woven wire filter, and the second layer adopts 5 ~ 20 order screen cloths to filter, and third layer adopts 60-70 order screen cloth to filter.
8. according to the arbitrary described hatching m of claim 1-7, it is characterized in that, described hatching operation also comprises:
The output time phase difference ovum of 1-2 days, can be used as and hatch with a collection of being placed in same described hatchery, and the output time can be placed at ovum on the same day samely describedly anti-escapes in hatching frame;
Diligent inspection between the incubation period, flood, slime water can not enter pond, prevent pieces of an egg from piling up overlapping, Timing measurement water temperature, temperature, observe water quality and embryonic development situation, make a record, and pinpoint the problems and process in time.
9. according to the arbitrary described hatching m of claim 1-8, it is characterized in that, the tadpole after described membrane is cultivated and is: just hatching small tadpole yolk out does not also absorb completely, and health is tiny, also should not shift out described anti-escaping and hatch frame, need to support about 10 days temporarily in hatching frame described anti-escaping; Firm membrane 3 days tadpoles do not ingest, by absorbing yolk growth; The complete rear tadpole of absorption of yolk at its ingests egg membrane, does not need to throw something and feed; After 7 days, throw something and feed 1 yolk that boils of each ten thousand tail supplements the nutrients, training ingestion ability; When tadpole grows to 2-3cm, tadpole has certain Burden-Swimming Ability of KM, independently can ingest and just can put into the cultivation of tadpole pond.
CN201510125493.6A 2015-03-20 2015-03-20 A kind of hatching method of Ranaspinosa David. Rana ovum Expired - Fee Related CN104770327B (en)

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CN106719429A (en) * 2017-01-19 2017-05-31 江西省林业科学院 A kind of hatching method in batches of Ranaspinosa David frog's egg
CN109247301A (en) * 2018-11-27 2019-01-22 湖南小新农业开发有限责任公司 A kind of cultural method of Rana nigromaculata

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