CN104758319A - Method for preparing traditional Chinese medicine extracting component by multienzyme enzymolysis - Google Patents
Method for preparing traditional Chinese medicine extracting component by multienzyme enzymolysis Download PDFInfo
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- CN104758319A CN104758319A CN201510148375.7A CN201510148375A CN104758319A CN 104758319 A CN104758319 A CN 104758319A CN 201510148375 A CN201510148375 A CN 201510148375A CN 104758319 A CN104758319 A CN 104758319A
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Abstract
The invention discloses a method for a traditional Chinese medicine extracting component by multienzyme enzymolysis. The method comprises the following steps: taking and cutting clean traditional Chinese medicine material into slices which are 5mm in thick, crushing, putting traditional Chinese medicine powder into a steam explosion tank, introducing nitrogen gas until the pressure in the steam explosion tank is 0.7-1.3MPa, and performing explosion treatment for 8-25 minutes; then, quickly introducing steam until the pressure in the steam explosion tank is 1.6-1.7MPa, performing steam explosion treatment for 0.5-2.5 minutes; putting the traditional Chinese medicine powder into an enzymolysis tank, adding a biological compound enzyme preparation comprising cellulose, protease, pectinase, glucamylase, xylanase and laccase after adding pure water, adding an organic solvent 10-15 times the amount of the biological compound enzyme preparation after enzymolysis is ended, leaching for 2-3 times, filtering, and combining filtrate; and drying to obtain powder. The method is simple, convenient and easy to operate and high in extraction rate.
Description
Technical field
The present invention relates to technical field of enzyme engineering, be specifically related to a kind of multienzyme enzymolysis and make the method that Chinese crude drug extracts composition.
Background technology
At present, Chinese crude drug extracting method has decocting method, circumfluence method, room temperature immersion method, ultrasonic extraction, microwave loss mechanisms, supercritical water extraction method and Subcritical water chromotagraphy method etc.
It is the more general method that plant produced uses that decocting method and circumfluence method are extracted, but its Extracting temperature is higher, and cause power consumption high, paste-forming rate is high, and the post-processed prepared to preparation makes troubles, and improves production cost.Ultrasonic extraction, microwave loss mechanisms, supercritical water extraction method, Subcritical water chromotagraphy method are all extraction processes newer recently.Supersound extraction is general with other extractive technique connected applications, existing process units, but distance large-scale industrial application distance in addition, as: ultrasonic mechanism problem is to the adaptability problem of different herbal species and Optimum Operation parameter problem; In industrial ultrasonic extraction element, how the energy of ultrasonic field realizes being uniformly distributed and economy problems, i.e. the scale-up problem of this technology; The drug effect equivalence of the extract that the cmm of simple prescription obtained under high ultrasonic extraction ratio or compound extract and legal extracting method obtain and toxicity problem, the i.e. evaluation problem of technical advance; The safety criterion problem etc. of ultrasonic radiation equipment.Microwave loss mechanisms is the simple process condition experiment carried out for some single concrete extraction object due to it, and the problems such as its mechanism of action and the large equipment produced of adaptation still await further research, are only limitted to the laboratory research stage.Super-critical fluid extraction and Subcritical water chromotagraphy method Maturity not high.Although supercritical extraction possesses many advantages compared with other extracting method, yield and chemical composition are all widely different; Technique changes and the drug effect equivalence that causes and toxicity are the problems of answering primary study; Supercritical extraction unit belongs to high pressure equipment, there is the problems such as the higher and safety of cost of equipment, also should note when applying.Meanwhile, they are high to equipment precision requirement, high in cost of production feature, realize in enterprise, carry out large need of production and more drop into.。
Meanwhile, existing active ingredients from traditional Chinese medicinal extracting method, ubiquity extraction ratio is low, biological activity is low, residue can not the shortcoming of cycling and reutilization.
Summary of the invention
For solving the problem, the invention provides a kind of multienzyme enzymolysis and make the method that Chinese crude drug extracts composition, simple process is easy to operate, and extraction ratio is high.
For achieving the above object, the technical scheme that the present invention takes is:
Multienzyme enzymolysis makes the method that Chinese crude drug extracts composition, comprises the steps:
S1, get and dryly strive after Chinese crude drug is cut into the lamellar of 5 mm of thickness, pulverize, cross 100 ~ 400 order medicines sieves;
S2, be placed in steam-explosion jar by the traditional Chinese medicine powder of step S1 gained, first passing into pressure in nitrogen to steam-explosion jar is 0.7 ~ 1.3MPa, explosion treatment 8 ~ 25min; Then passing into rapidly pressure in steam to steam-explosion jar is 1.3 ~ 1.7MPa, Steam explosion treatment 0.5 ~ 2.5min;
S3, the raw material of step S2 gained is placed in enzymatic vessel, ratio in 1: 8 ~ 1: 12 add pure strive water after, the compound bio-enzyme preparation comprising cellulase, protease, pectase, glucoamylase, xylanase, laccase is added in the ratios of 80.0 ~ 100.0 milliliters/ton, 30 ~ 40 DEG C, enzymolysis 72 hours, stirring in every 4 hours, oxygenation are once;
S4, by the enzymatic hydrolysate of step S3 gained, carry out enzyme denaturing process, obtain Chinese crude drug slurry, add the organic solvent (with the weight of initial Chinese crude drug for radix) of 10 ~ 15 times, lixiviate 2 ~ 3 times, filter, merging filtrate;
S5, the filtrate of step S4 gained is carried out spraying dry, baking temperature is no more than 65 DEG C, and moisture Control, below 7%, obtains powder body.
Wherein, described organic solvent is the turpentine derivatives of natural origin.
Wherein, described turpentine derivatives is isomery, the disproportionation products of pinene.
Wherein, the ratio of described cellulase, protease, pectase, glucoamylase, xylanase, laccase is 3: 1: 2: 1: 2: 1.
Wherein, in described step S4, organic solvent extraction temperature is 60 ~ 75 DEG C, and the time is 0.5 ~ 1.5 hour.
The present invention has following beneficial effect:
First by lignin that the pectin in Chinese crude drug cell wall is inlayed by steam explosion technology, hemicellulose and cellulosic copolymer are broken up as lignin, pectin, hemicellulose and cellulosic dispersion, thus destroy the lignin natural cover for defense system that compound bio-enzyme is difficult to rapidly degraded, and to active ingredients from traditional Chinese medicinal without any destruction, again by compound enzyme by lignin, pectin, hemicellulose, protein and cellulose etc. are degraded to micromolecule, reduce the interference that in active ingredients from traditional Chinese medicinal leaching process, macromolecular complex confrontation is extracted, further increase extraction ratio, adopt plant organic solvent, recyclable, meet industry aluminium extrusions requirement.
Detailed description of the invention
In order to make objects and advantages of the present invention clearly understand, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
Embodiment
In following examples, compound bio-enzyme preparation mixes in the ratio of 3: 1: 2: 1: 2: 1 by cellulase, protease, pectase, glucoamylase, xylanase, laccase.Organic solvent is the turpentine derivatives of natural origin, and turpentine derivatives is isomery, the disproportionation products of pinene.
Embodiment 1
S1, get after clean Chinese crude drug is cut into the lamellar of 5 mm of thickness, pulverize, cross 100 order medicines sieves;
S2, be placed in steam-explosion jar by the traditional Chinese medicine powder of step S1 gained, first passing into pressure in nitrogen to steam-explosion jar is 0.7MPa, explosion treatment 8min; Then passing into rapidly pressure in steam to steam-explosion jar is 1.3MPa, Steam explosion treatment 0.5min;
S3, the raw material of step S2 gained is placed in enzymatic vessel, after ratio in 1: 8 adds pure water, the compound bio-enzyme preparation comprising cellulase, protease, pectase, glucoamylase, xylanase, laccase is added in the ratios of 80.0 milliliters/ton, 30 DEG C, enzymolysis 72 hours, stirring in every 4 hours, oxygenation are once;
S4, by the enzymatic hydrolysate of step S3 gained, carry out enzyme denaturing process, obtain Chinese crude drug slurry, add the organic solvent (with the weight of initial Chinese crude drug for radix) of 10 times, 60 DEG C, lixiviate 2 times, the time is 0.5 hour, filters, merging filtrate;
S5, the filtrate of step S4 gained is carried out spraying dry, baking temperature is no more than 65 DEG C, and moisture Control, below 7%, obtains powder body.
Embodiment 2
S1, get after clean Chinese crude drug is cut into the lamellar of 5 mm of thickness, pulverize, cross 400 order medicines sieves;
S2, be placed in steam-explosion jar by the traditional Chinese medicine powder of step S1 gained, first passing into pressure in nitrogen to steam-explosion jar is 1.3MPa, explosion treatment 25min; Then passing into rapidly pressure in steam to steam-explosion jar is 1.7MPa, Steam explosion treatment 2.5min;
S3, the raw material of step S2 gained is placed in enzymatic vessel, ratio in 1: 12 add pure strive water after, the compound bio-enzyme preparation comprising cellulase, protease, pectase, glucoamylase, xylanase, laccase is added in the ratios of 100.0 milliliters/ton, 40 DEG C, enzymolysis 72 hours, every 44, time stir, oxygenation once;
S4, by the enzymatic hydrolysate of step S3 gained, carry out enzyme denaturing process, obtain Chinese crude drug slurry, add the organic solvent (with the weight of initial Chinese crude drug for radix) of 15 times, 75 DEG C, lixiviate 3 times, 1.5 hours time, filter, merging filtrate;
S5, the filtrate of step S4 gained is carried out spraying dry, baking temperature is no more than 65 DEG C, and moisture Control, below 7%, obtains powder body.
Embodiment 3
S1, get after clean Chinese crude drug is cut into the lamellar of 5 mm of thickness, pulverize, cross 250 order medicines sieves;
S2, be placed in steam-explosion jar by the traditional Chinese medicine powder of step S1 gained, first passing into pressure in nitrogen to steam-explosion jar is 1MPa, explosion treatment 16.5min; Then passing into rapidly pressure in steam to steam-explosion jar is 1.5MPa, Steam explosion treatment 1.5min;
S3, the raw material of step S2 gained is placed in enzymatic vessel, after ratio in 1: 10 adds pure water, the compound bio-enzyme preparation comprising cellulase, protease, pectase, glucoamylase, xylanase, laccase is added in the ratios of 90 milliliters/ton, 35 DEG C, enzymolysis 72 hours, stirring in every 4 hours, oxygenation are once;
S4, by the enzymatic hydrolysate of step S3 gained, carry out enzyme denaturing process, obtain Chinese crude drug slurry, add the organic solvent (with the weight of initial Chinese crude drug for radix) of 12.5 times, 67.5 DEG C, the time is 1 hour, lixiviate 3 times, filters, merging filtrate;
S5, the filtrate of step S4 gained is carried out spraying dry, baking temperature is no more than 65 DEG C, and moisture Control, below 7%, obtains powder body.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (5)
1. Chinese crude drug multienzyme enzymolysis makes the method that Chinese crude drug extracts composition, it is characterized in that, comprises the steps:
S1, get after clean Chinese crude drug is cut into the lamellar of 5 mm of thickness, pulverize, cross 100 ~ 400 order medicines sieves;
S2, be placed in steam-explosion jar by the traditional Chinese medicine powder of step S1 gained, first passing into pressure in nitrogen to steam-explosion jar is 0.7 ~ 1.3MPa, explosion treatment 8 ~ 25min; Then passing into rapidly pressure in steam to steam-explosion jar is 1.3 ~ 1.7MPa, Steam explosion treatment 0.5 ~ 2.5min;
S3, the raw material of step S2 gained is placed in enzymatic vessel, after ratio in 1: 8 ~ 1: 12 adds pure water, the compound bio-enzyme preparation comprising cellulase, protease, pectase, glucoamylase, xylanase, laccase is added in the ratios of 80.0 ~ 100.0 milliliters/ton, 30 ~ 40 DEG C, enzymolysis 72 hours, stirring in every 4 hours, oxygenation are once;
S4, by the enzymatic hydrolysate of step S3 gained, carry out enzyme denaturing process, obtain Chinese crude drug slurry, add the organic solvent of 10 ~ 15 times, lixiviate 2 ~ 3 times, filter, merging filtrate;
S5, the filtrate of step S4 gained is carried out spraying dry, baking temperature is no more than 65 DEG C, and moisture Control, below 7%, obtains powder body.
2. multienzyme enzymolysis according to claim 1 makes the method that Chinese crude drug extracts composition, and it is characterized in that, described organic solvent is the turpentine derivatives of natural origin.
3. multienzyme enzymolysis according to claim 2 makes the method that Chinese crude drug extracts composition, and it is characterized in that, described turpentine derivatives is isomery, the disproportionation products of pinene.
4. multienzyme enzymolysis according to claim 1 makes the method that Chinese crude drug extracts composition, and it is characterized in that, the ratio of described cellulase, protease, pectase, glucoamylase, xylanase, laccase is 3: 1: 2: 1: 2: 1.
5. multienzyme enzymolysis according to claim 1 makes the method that Chinese crude drug extracts composition, and it is characterized in that, in described step S4, organic solvent extraction temperature is 60 ~ 75 DEG C, and the time is 0.5 ~ 1.5 hour.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105254773A (en) * | 2015-10-27 | 2016-01-20 | 齐齐哈尔医学院 | Preparation method of antitumor polysaccharide |
| CN105255962A (en) * | 2015-10-23 | 2016-01-20 | 齐齐哈尔医学院 | Method for extracting blood sugar-reducing panaxan by enzyme method and application of obtained panaxan |
| CN105709209A (en) * | 2016-01-17 | 2016-06-29 | 新乡医学院第一附属医院 | Medicine for treating tumor and preparation method thereof |
| CN106047540A (en) * | 2016-08-06 | 2016-10-26 | 张新红 | Home-brew beer and preparation method thereof |
| CN106177175A (en) * | 2016-08-24 | 2016-12-07 | 张东 | A kind of wolfberry fruit extract, the pharmaceutical preparation and preparation method thereof of antifatigue effect |
| CN107668698A (en) * | 2017-09-29 | 2018-02-09 | 西藏青稞麦绿素生物科技有限公司 | A kind of preparation method of barley green |
| CN107815375A (en) * | 2017-12-12 | 2018-03-20 | 湖北工业大学 | Citrinin content and the method for preparing wine of rice fermented with red yeast in a kind of reduction red yeast rice |
| CN108902588A (en) * | 2018-08-31 | 2018-11-30 | 广西宏邦食品有限公司 | A kind of production method of Siraitia grosvenorii plant beverage |
| CN111066928A (en) * | 2019-12-20 | 2020-04-28 | 郑州工程技术学院 | Preparation method of wild jujube leaf tea |
| CN111454767A (en) * | 2020-04-27 | 2020-07-28 | 合肥学院 | Method for extracting folium artemisiae argyi essential oil |
| CN114731904A (en) * | 2022-05-05 | 2022-07-12 | 桂林市农业科学研究中心 | Cultivation bag material for improving yield and quality of clitocybe maxima and preparation method thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101732359A (en) * | 2008-11-11 | 2010-06-16 | 中国科学院过程工程研究所 | Integrated method for poly-generation of Chinese medicinal extracts |
| CN102600222A (en) * | 2012-04-13 | 2012-07-25 | 吉林农业大学 | Method for increasing content of rare saponin in ginseng raw material by explosion of steam |
| CN102614236A (en) * | 2012-04-13 | 2012-08-01 | 吉林农业大学 | Method for processing raw materials of ginseng original drug, food and health care products |
| CN102618616A (en) * | 2012-04-13 | 2012-08-01 | 吉林农业大学 | Method of improving ginsenoside extraction rate by utilizing steam-explosion combined bio-composite enzyme technology |
-
2015
- 2015-03-28 CN CN201510148375.7A patent/CN104758319A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101732359A (en) * | 2008-11-11 | 2010-06-16 | 中国科学院过程工程研究所 | Integrated method for poly-generation of Chinese medicinal extracts |
| CN102600222A (en) * | 2012-04-13 | 2012-07-25 | 吉林农业大学 | Method for increasing content of rare saponin in ginseng raw material by explosion of steam |
| CN102614236A (en) * | 2012-04-13 | 2012-08-01 | 吉林农业大学 | Method for processing raw materials of ginseng original drug, food and health care products |
| CN102618616A (en) * | 2012-04-13 | 2012-08-01 | 吉林农业大学 | Method of improving ginsenoside extraction rate by utilizing steam-explosion combined bio-composite enzyme technology |
Non-Patent Citations (1)
| Title |
|---|
| 张娟 等: ""物理_生物法耦联工艺提高甘草有效成分的研究"", 《时珍国医国药》 * |
Cited By (13)
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| CN105255962A (en) * | 2015-10-23 | 2016-01-20 | 齐齐哈尔医学院 | Method for extracting blood sugar-reducing panaxan by enzyme method and application of obtained panaxan |
| CN105254773A (en) * | 2015-10-27 | 2016-01-20 | 齐齐哈尔医学院 | Preparation method of antitumor polysaccharide |
| CN105709209A (en) * | 2016-01-17 | 2016-06-29 | 新乡医学院第一附属医院 | Medicine for treating tumor and preparation method thereof |
| CN106047540A (en) * | 2016-08-06 | 2016-10-26 | 张新红 | Home-brew beer and preparation method thereof |
| CN106177175A (en) * | 2016-08-24 | 2016-12-07 | 张东 | A kind of wolfberry fruit extract, the pharmaceutical preparation and preparation method thereof of antifatigue effect |
| CN107668698A (en) * | 2017-09-29 | 2018-02-09 | 西藏青稞麦绿素生物科技有限公司 | A kind of preparation method of barley green |
| CN107815375A (en) * | 2017-12-12 | 2018-03-20 | 湖北工业大学 | Citrinin content and the method for preparing wine of rice fermented with red yeast in a kind of reduction red yeast rice |
| CN107815375B (en) * | 2017-12-12 | 2020-10-30 | 湖北工业大学 | Method for reducing citrinin content in red rice and preparing red rice wine |
| CN108902588A (en) * | 2018-08-31 | 2018-11-30 | 广西宏邦食品有限公司 | A kind of production method of Siraitia grosvenorii plant beverage |
| CN111066928A (en) * | 2019-12-20 | 2020-04-28 | 郑州工程技术学院 | Preparation method of wild jujube leaf tea |
| CN111066928B (en) * | 2019-12-20 | 2022-06-03 | 郑州工程技术学院 | Preparation method of wild jujube leaf tea |
| CN111454767A (en) * | 2020-04-27 | 2020-07-28 | 合肥学院 | Method for extracting folium artemisiae argyi essential oil |
| CN114731904A (en) * | 2022-05-05 | 2022-07-12 | 桂林市农业科学研究中心 | Cultivation bag material for improving yield and quality of clitocybe maxima and preparation method thereof |
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Application publication date: 20150708 |