CN104749293A - Method for efficiently extracting carotenoids in yellow peach fruits and determining carotenoids in yellow peach fruits by liquid phase - Google Patents
Method for efficiently extracting carotenoids in yellow peach fruits and determining carotenoids in yellow peach fruits by liquid phase Download PDFInfo
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Abstract
The invention discloses a method for efficiently extracting carotenoids in yellow peach fruits and determining the carotenoids in the yellow peach fruits by a liquid phase. Extracting conditions are as follows: the material-liquid ratio of pulp to an extracting agent acetone is 1 to 6 (g/mL), and ultrasonic light-shielding assisted extraction is carried out for 1 hour; chromatographic conditions are as follows: a YMC-C30 type chromatographic column is adopted; a mobile phase comprises methyl tert-butyl ether methanol with a ratio being 30 to 70; an isocratic elution manner is adopted; a flow speed is equal to 1.0mL/min; the wavelength is 450nm; the column temperature is 25 DEG C; a sample feeding amount is 20 microliters; and the time lasts for 18 minutes. According to the method for efficiently extracting the carotenoids in the yellow peach fruits and determining the carotenoids in the yellow peach fruits by the liquid phase, the extraction material-liquid ratio is 1 to 6 (g/mL), the ultrasonic light-shielding assisted extraction is carried out for 1 hour and the methyl tert-butyl ether and the methanol with the ratio of 30 to 70 are used as the mobile phase for carrying out the isocratic elution; the three conditions are combined so that a plurality of types of carotenoids in the yellow peach fruits can be effectively and completely extracted and rapidly and accurately determined; and the method is easy to operate, free of saponification and gradient elution, good in repeatability of a determined result, high in accuracy and short in liquid phase time.
Description
Technical field
The invention belongs to the chemical analysis in chemical industry and instrument analysis technology field.
Background technology
Yellow meat peach can be eaten raw with canning processing dual-purpose, and economic worth and researching value are all higher, and carotenoid is one of polyphenoils important in yellow meat Peach fruits.According to existing document, the extraction extracting method of the disclosed carotenoid in plants such as vegetables and fruit is loaded down with trivial details at present, usually need saponification process, and extraction effect is bad, and the complicated condition of high-performance liquid chromatogram determination, mobile phase composition and ratio used and gradient elution process all very complicated, measure composition single, the working sample time is long, and cost is high.Therefore, in order to carry out effective evaluation and utilization to yellow meat peach resource, the method setting up multiple types carrotene in the effective abstraction and quantification of a kind of fast, economical yellow meat peach pulp is particularly important.
A small amount of document utilization YMC-C30 chromatographic column is had to carry out the separation of carotenoid in plants at present, directly utilize the extractions such as methyl alcohol, chloroform or acetone, directly measure without the need to saponification, but all solid-liquid ratio when extracting and concrete extracting mode and time are not accurately limited; And be all utilize the reagent such as acetonitrile, methyl alcohol, methyl tert-butyl ether and water to carry out complicated proportioning combination, thus carry out the gradient elution of several mobile phase, must configure 2 yuan of pumps even 4 yuan of pumps just can satisfy the demands, trivial operations, instrument requirements is high, and minute is long, cost is high, measure limited (" high performance liquid chromatography detects the curcumin and carotenoid compounds that add in starch food products simultaneously ", Yang Fang etc., 2014; " close colored thorny elaeagnus fruit major carotenoids component and content analysis ", Hu Haitao etc., 2014; " in tomato products the C_(30-of all-trans lycopene and content beta-carotene) HPLC inner mark method ration is analyzed ", Ding Jing etc., 2010; " research of Peach fruits carotenoid assay method ", Yan Shaobin etc., 2012; " mensuration of Beijing area Vegetables Lutein, luteole and beta carotene and content thereof ", prince's sunrise and Lin Xiaoming, 2010).
Particularly, document " research of Peach fruits carotenoid assay method " discloses Peach fruits carotenoid and directly uses acetone extraction, measures, adopt 4 DEG C of extracted many times during extraction without the need to saponification extracting directly, last constant volume is to 25mL, and namely its solid-liquid ratio is 1:12.5; During upper high-performance liquid chromatogram determination, it adopts methyl alcohol (A), methyl tert-butyl ether (B) and water (C) three kinds of reagent as mobile phase, and carry out gradient elution, elution requirement is: 0 ~ 90min, 81%A15%B4%C ~ 6%90%B4%C; 92min, 81%A15%B4%C; A sample 100min consuming time.The method mobile phase composition and gradient elution process very complicated, and length consuming time, cost is high.
In addition, patent documentation CN102507775A discloses with mobile phase methyl tert-butyl ether: methyl alcohol=15:85 isocratic elution detects single carotenoid, i.e. the content of xenthophylls.
Summary of the invention
The object of this invention is to provide a kind of method that high efficiency extraction and liquid phase measure carotenoid in yellow meat Peach fruits.
For achieving the above object, the technical solution used in the present invention is: a kind of high efficiency extraction and liquid phase measure the method for carotenoid in yellow meat Peach fruits, and its step comprises:
The mixed standard solution of a, formulation of carotenoids, drawing standard solution curve;
B, extract from yellow meat peach pulp carotenoid preparation sample solution;
C, under chromatographic condition, efficient liquid phase chromatographic analysis is carried out to sample solution, and detect with VWD detecting device;
D, the analysis result of sample solution and typical curve to be compared, draw the carotenoid content in sample solution;
It is characterized in that:
Extraction conditions in step b is:
Extraction agent: acetone;
Extracting mode: ultrasonic assistant extracts;
Solid-liquid ratio: the solid-liquid ratio of yellow meat peach pulp and acetone is 1:6(g/mL);
Extraction time: 1h;
Directly with the solvent of acetone as standard solution and sample solution;
Chromatographic condition in step c is:
Chromatographic column: YMC-C30;
Mobile phase: methyl tert-butyl ether: the volume ratio of methyl alcohol is 30:70;
Type of elution: isocratic elution;
Flow velocity: 1.0 mL/min;
Wavelength: 450 nm;
Column temperature: 25 DEG C;
Sample size: 20 μ L;
Time: 18 min.
Further, the step preparing the mixed standard solution of 5 Carotenoids in step a is: accurately take each carotenoid standard items of 1mg respectively, and with 10 mL acetone solution constant volumes, obtained concentration is the standard solution of 100 mg/L; Pipette each standard solution of 1.00 mL respectively, mix and use acetone stepwise dilution, obtained each concentration list mark and each concentration mixed standard solution.
Further, the step extracting carotenoid preparation sample solution in its step b from pulp is: take pulp, according to solid-liquid ratio 1:6(g/mL) add acetone, lucifuge ultrasonic assistant extracts 1h, centrifugal 10 min of 10000 rpm at 4 DEG C, get supernatant and namely obtain sample solution, filter through the organic filter of 0.22 mm.
Further, the carotenoid that drawing standard solution uses comprises xenthophylls, luteole, beta-cryptoxanthin, alpha-carotene, beta carotene.
High efficiency extraction of the present invention and liquid phase measure the method for carotenoid in yellow meat Peach fruits, extract solid-liquid ratio 1:6(g/mL), ultrasonic assistant extracts 1h, adopt methyl tert-butyl ether: methyl alcohol=30:70 is as mobile phase isocratic elution, and three conditions combine the multiple types carrotene that can extract effectively completely with in rapid and accurate determination yellow meat peach pulp, simple to operate, without the need to saponification without the need to gradient elution, measurement result is reproducible, and accuracy is high, and the liquid phase used time is short.The extraction thinking determined and chromatographic condition also can be used for the carotenoid in other fruits and vegetables of separation determination.
Accompanying drawing explanation
Fig. 1 is the HPLC figure of mixed standard solution in embodiment 1, wherein 1. xenthophylls; 2. luteole; 3. beta-cryptoxanthin; 4. alpha-carotene; 5. beta carotene.
Fig. 2 is the HPLC figure of carotenoid in ' Nanjing Huang dew ' kind Meat Sample in embodiment 1, wherein 1. xenthophylls; 2. luteole; 3. beta-cryptoxanthin; 4. alpha-carotene; 5. beta carotene.
Fig. 3 is in embodiment 2
'beautiful ' the HPLC figure of carotenoid in kind Meat Sample, wherein 1. xenthophylls; 2. luteole; 3. beta-cryptoxanthin; 4. alpha-carotene; 5. beta carotene.
Fig. 4 is in embodiment 3
'no. 20, auspicious light ' the HPLC figure of carotenoid in kind Meat Sample, wherein 1. xenthophylls; 2. luteole; 3. beta-cryptoxanthin; 4. alpha-carotene; 5. beta carotene.
Fig. 5 is chromatogram in comparative example 1.Wherein 1. xenthophylls; 2. luteole; 3. beta-cryptoxanthin; 4. alpha-carotene; 5. beta carotene.
Fig. 6 is chromatogram in comparative example 2.Wherein 1. xenthophylls; 2. luteole; 3. beta-cryptoxanthin; 4. alpha-carotene; 5. beta carotene.
Fig. 7 is chromatogram in comparative example 3.Wherein 1. xenthophylls; 2. luteole; 3. beta-cryptoxanthin; 4. alpha-carotene; 5. beta carotene.
Fig. 8 is chromatogram in comparative example 4.Wherein 1. xenthophylls; 2. luteole; 3. beta-cryptoxanthin; 4. alpha-carotene; 5. beta carotene.
Fig. 9 is chromatogram in comparative example 5.Wherein 1. xenthophylls; 2. luteole; 3. beta-cryptoxanthin; 4. alpha-carotene; 5. beta carotene.
Figure 10 is comparative example 6 and embodiment 1 ' Nanjing Huang dew ' peach fruit flesh carrotene compound mensuration result under different feed liquid ratio.
Figure 11 is that comparative example 7 and embodiment 1 adopt different extracting mode and extraction time ' Nanjing Huang dew ' peach pulp two kinds of major carotenoids assay results.
Below in conjunction with accompanying drawing, embodiments of the present invention are described further.
Embodiment
Reagent and equipment:
5 Carotenoids standard items comprise xenthophylls, luteole, beta-cryptoxanthin, alpha-carotene, beta carotene, equal available from Sigma, purity >=95%.
Ultrasonic cleaner, hydro-extractor, Agilent highly effective liquid phase chromatographic system: 1100 series, VWD UV-detector, YMC-C30 stratographic analysis post (4.6 × 250 mm, 5 μm).
embodiment 1
A, the step of standard solution preparing 5 Carotenoids are: accurately take each carotenoid standard items of 1mg respectively, with 10 mL acetone solution constant volumes, obtained concentration is the standard solution of 100 mg/L; Pipette each standard solution of 1.00 mL respectively, mix and use acetone stepwise dilution, obtained each concentration list mark and each concentration mixed standard solution.Getting concentration is that the mixed standard solution of 10mg/L obtains standard solution curve through efficient liquid phase chromatographic analysis, and see Fig. 1, chromatographic condition is:
Chromatographic column: YMC-C30;
Mobile phase: methyl tert-butyl ether: methyl alcohol=30:70;
Type of elution: isocratic elution;
Flow velocity: 1.0 mL/min;
Wavelength: 450 nm;
Column temperature: 25 DEG C;
Sample size: 20 μ L;
Time: 15 min;
B, accurately take 0.5g ' Nanjing Huang dew ' kind ripening fruits pulp, add 3mL acetone, lucifuge ultrasound wave extracts 1 h, centrifugal 10 min of 10000 rpm at 4 DEG C, gets supernatant and obtain sample solution after the organic filter of 0.22 mm filters;
C, carry out efficient liquid phase chromatographic analysis to sample solution, obtain the chromatogram of sample solution, see Fig. 2, chromatographic condition is with the chromatographic condition of typical curve;
D, by the chromatogram of sample solution and standard solution curve comparison, obtain the content of various types of carrotene in ' Nanjing Huang dew ' peach pulp, in table 1, unit is mg/kg, the content namely contained in every kg pulp.
embodiment 2
Accurately take 0.5g ' beautiful ' kind ripening fruits pulp, add 3mL acetone, lucifuge ultrasound wave extracts 1 h, centrifugal 10 min of 10000 rpm at 4 DEG C, get supernatant after the organic filter of 0.22 mm filters, to obtain sample solution enter chromatographic column, chromatographic condition is with embodiment 1, and the chromatogram of gained sample solution is shown in Fig. 3, and in peach pulp, the content of various types of carrotene is in table 1.
embodiment 3
Accurately take No. 20, the auspicious light of 0.5g ' ' kind ripening fruits pulp, add 3mL acetone, lucifuge ultrasound wave extracts 1 h, centrifugal 10 min of 10000 rpm at 4 DEG C, get supernatant after the organic filter of 0.22 mm filters, to obtain sample solution enter chromatographic column, chromatographic condition is with embodiment 1, and the chromatogram of gained sample solution is shown in Fig. 4, and in peach pulp, the content of various types of carrotene is in table 1.
comparative example 1
The step preparing the standard solution of 5 Carotenoids is: accurately take each carotenoid standard items of 1mg respectively, and with 10 mL acetone solution constant volumes, obtained concentration is the standard solution of 100 mg/L; Pipette each standard solution of 1.00 mL respectively, mix and use acetone stepwise dilution, obtained each concentration list mark and each concentration mixed standard solution.Getting concentration is that the mixed standard solution of 10mg/L obtains standard solution curve through efficient liquid phase chromatographic analysis, and see Fig. 5, chromatographic condition is:
Chromatographic column: YMC-C30;
Mobile phase: methyl tert-butyl ether: methyl alcohol=50:50;
Type of elution: isocratic elution;
Flow velocity: 1.0 mL/min;
Wavelength: 450 nm;
Column temperature: 25 DEG C;
Sample size: 20 μ L;
Time: 10 min.
Relative embodiment 1, comparative example 1 reduces the ratio of mobile phase methanol, cannot be separated hybrid standard product 5 Carotenoids, visible the inventive method is by adopting methyl tert-butyl ether: the proportioning of methyl alcohol=30:70 can reach the effect of this 5 Carotenoids of fully effective separation.
comparative example 2
The step preparing the standard solution of 5 Carotenoids is: accurately take each carotenoid standard items of 1mg respectively, and with 10 mL acetone solution constant volumes, obtained concentration is the standard solution of 100 mg/L; Pipette each standard solution of 1.00 mL respectively, mix and use acetone stepwise dilution, obtained each concentration list mark and each concentration mixed standard solution.Getting concentration is that the mixed standard solution of 10mg/L obtains standard solution curve through efficient liquid phase chromatographic analysis, and see Fig. 6, chromatographic condition is:
Chromatographic column: YMC-C30;
Mobile phase: methyl tert-butyl ether: methyl alcohol=40:60;
Type of elution: isocratic elution;
Flow velocity: 1.0 mL/min;
Wavelength: 450 nm;
Column temperature: 25 DEG C;
Sample size: 20 μ L;
Time: 15 min.
Relative embodiment 1, comparative example 2 reduces the ratio of mobile phase methanol, low and cannot effectively be separated to the response of hybrid standard product 5 Carotenoids, visible the inventive method is by adopting methyl tert-butyl ether: the proportioning of methyl alcohol=30:70 can reach the effect of this 5 Carotenoids of fully effective separation.
comparative example 3
The step preparing the standard solution of 5 Carotenoids is: accurately take each carotenoid standard items of 1mg respectively, and with 10 mL acetone solution constant volumes, obtained concentration is the standard solution of 100 mg/L; Pipette each standard solution of 1.00 mL respectively, mix and use acetone stepwise dilution, obtained each concentration list mark and each concentration mixed standard solution.Getting concentration is that the mixed standard solution of 10mg/L obtains standard solution curve through efficient liquid phase chromatographic analysis, and see Fig. 7, chromatographic condition is:
Chromatographic column: YMC-C30;
Mobile phase: methyl tert-butyl ether: methyl alcohol=15:85;
Type of elution: isocratic elution;
Flow velocity: 1.0 mL/min;
Wavelength: 450 nm;
Column temperature: 25 DEG C;
Sample size: 20 μ L;
Time: 100 min.
The method of comparative example 3 referenced patents document CN102507775A has also made any amendment, be about to measure 450 nm that wavelength 445nm changes the detection of suitable multiple types carrotene into, relative embodiment 1, increase the ratio of methyl alcohol, the each carotenoid of hybrid standard product can effectively be separated, but response is low, minute extends 3.5 times.Visible the present invention is by adopting methyl tert-butyl ether: the proportioning of methyl alcohol=30:70, can effectively be separated each carotenoid, increase response, shorten minute.
comparative example 4
The step preparing the standard solution of 5 Carotenoids is: accurately take each carotenoid standard items of 1mg respectively, and with 10 mL acetone solution constant volumes, obtained concentration is the standard solution of 100 mg/L; Pipette each standard solution of 1.00 mL respectively, mix and use acetone stepwise dilution, obtained each concentration list mark and each concentration mixed standard solution.Getting concentration is that the mixed standard solution of 10mg/L obtains standard solution curve through efficient liquid phase chromatographic analysis, and see Fig. 8, chromatographic condition is:
Chromatographic column: YMC-C30;
Mobile phase: methyl alcohol: methyl tert-butyl ether: water=81:15:4;
Type of elution: gradient elution;
Condition of gradient elution: 0 ~ 90min, 81%A15%B4%C ~ 6%90%B4%C; 92min, 81%A15%B4%C;
Flow velocity: 1.0 mL/min;
Wavelength: 450 nm;
Column temperature: 25 DEG C;
Sample size: 20 μ L;
Time: 40 min.
Comparative example 4 quotes the method for article " research of Peach fruits carotenoid assay method ", and relative embodiment 1 changes composition and ratio and the gradient of mobile phase, and its proportion of mobile phase of relative embodiment 1 is complicated, and gradient elution is complicated.The each carotenoid of hybrid standard product can effectively be separated, minute extends 2 times, response is lower than embodiment 1, visible the present invention is by adopting methyl tert-butyl ether: methyl alcohol=30:70 is as single mobile phase, can effectively be separated each carotenoid, simplify checking procedure, shorten minute.
comparative example 5
Accurately take 0.5g ' Nanjing Huang dew ' kind ripening fruits pulp, add 3mL acetone, lucifuge ultrasound wave extracts 1 h, centrifugal 10 min of 10000 rpm at 4 DEG C, gets supernatant and obtain sample solution after the organic filter of 0.22 mm filters.
Carry out efficient liquid phase chromatographic analysis to sample solution, obtain the chromatogram of sample solution, see Fig. 9, chromatographic condition is: chromatographic column: YMC-C30;
Mobile phase: methyl alcohol: methyl tert-butyl ether: water=81:15:4;
Type of elution: gradient elution;
Condition of gradient elution: 0 ~ 90min, 81%A15%B4%C ~ 6%90%B4%C; 92min, 81%A15%B4%C;
Flow velocity: 1.0 mL/min;
Wavelength: 450 nm;
Column temperature: 25 DEG C;
Sample size: 20 μ L;
Time: 70 min.
Comparative example 5 quotes the method for article " research of Peach fruits carotenoid assay method ", and relative embodiment 1 changes composition and ratio and the gradient of mobile phase, and its proportion of mobile phase of relative embodiment 1 is complicated, and gradient elution is complicated.Peach ' Nanjing Huang dew ' each carotenoid of kind can effectively be separated, and machine is long for stabilization time, and minute extends 3.5 times, and it is bad to measure effect, response is very low, and wherein the response of luteole is low especially, completely cannot compared with the mensuration effect of embodiment 1.
comparative example 6
Accurately take 0.5g ' Nanjing Huang dew respectively ' each 4 parts of kind ripening fruits pulp, add 1 respectively, 2,4,5mL acetone, namely solid-liquid ratio is respectively 1:2,1:4,1:8,1:10, lucifuge 4 DEG C of lixiviate 1h, centrifugal 10 min of 10000 rpm at 4 DEG C, get supernatant and obtain sample solution after the organic filter of 0.22 mm filter.Carry out efficient liquid phase chromatographic analysis to sample solution, chromatographic condition is with embodiment 1, and by the chromatogram of sample solution and standard solution curve comparison, obtain the content that various solid-liquid ratio extracts lower carotenoid, unit is mg/kg, the content namely contained in every kg pulp.
The result of comparative example 6 contrasts with the result in embodiment 1, obtains broken line graph Figure 10.Obviously, the less meeting of solid-liquid ratio causes extracting not exclusively, and the higher meeting of solid-liquid ratio causes concentration too low, cannot detect.During yellow meat peach Extraction of carotenoid pigment, solid-liquid ratio needs the value of relative constancy, and it is 1:6(g/mL that the present invention finds at solid-liquid ratio) time, the carotenoid contained in yellow meat peach can be extracted completely, and the carotenoid content in extract is applicable to measuring.
comparative example 7
Accurately take 0.5g ' Nanjing Huang dew respectively ' each 4 parts of kind ripening fruits pulp, add 3mL acetone respectively, namely solid-liquid ratio is 1:6, leave standstill ultrasound wave lucifuge assisted extraction and 4 DEG C respectively and extract 30min and 2h, centrifugal 10 min of 10000 rpm at 4 DEG C, get supernatant and obtain sample solution after the organic filter of 0.22 mm filter.Carry out efficient liquid phase chromatographic analysis to sample solution, chromatographic condition is with embodiment 1, and by the chromatogram of sample solution and standard solution curve comparison, obtain the content that various solid-liquid ratio extracts lower carotenoid, unit is mg/kg, the content namely contained in every kg pulp.
The result of comparative example 7 contrasts with the result in embodiment 1, and the luteole the abundantest with content in ' Nanjing Huang dew ' kind pulp and the content of beta carotene obtain column diagram Figure 11.Obviously, ultrasonic assistant extracts and is better than 4 DEG C of standing extractions, but time shorter meeting causes extracting not exclusively, and the time, longer meeting caused carotenoid to lose.In the present invention, ultrasonic assistant extracts 1h can reach best extraction effect.
Carotenoid compound mensuration result in each kind peach in table 1 embodiment 1 ~ 3
The result of the typical curve equation of linear regression of table 2 embodiment 1, related coefficient, minimum detectability, relative standard deviation and the recovery
After measured, the equation of linear regression of 5 Carotenoids, related coefficient, minimum detectability, relative standard deviation and the recovery the results are shown in Table 2.In the corresponding range of linearity, various types of carrotene linear relationship is good, and related coefficient all reaches more than 0.9999; RSD of the present invention is within 0.99 ~ 2.25%, and the recovery is 95 ~ 105%, and precision is higher, and result is accurate.
Claims (4)
1. high efficiency extraction and liquid phase measure a method for carotenoid in yellow meat Peach fruits, and its step comprises:
The mixed standard solution of a, formulation of carotenoids, drawing standard solution curve;
B, extract from yellow meat peach pulp carotenoid preparation sample solution;
C, under chromatographic condition, efficient liquid phase chromatographic analysis is carried out to sample solution, and detect with VWD detecting device;
D, the analysis result of sample solution and typical curve to be compared, draw the carotenoid content in sample solution;
It is characterized in that:
Extraction conditions in step b is:
Extraction agent: acetone;
Extracting mode: ultrasonic assistant extracts;
Solid-liquid ratio: the solid-liquid ratio of yellow meat peach pulp and acetone is 1:6(g/mL);
Extraction time: 1h;
Directly with the solvent of acetone as standard solution and sample solution;
Chromatographic condition in step c is:
Chromatographic column: YMC-C30;
Mobile phase: methyl tert-butyl ether: the volume ratio of methyl alcohol is 30:70;
Type of elution: isocratic elution;
Flow velocity: 1.0 mL/min;
Wavelength: 450 nm;
Column temperature: 25 DEG C;
Sample size: 20 μ L;
Time: 18 min.
2. high efficiency extraction according to claim 1 and liquid phase measure the method for carotenoid in yellow meat Peach fruits, it is characterized in that: the step preparing the mixed standard solution of 5 Carotenoids in step a is: accurately take each carotenoid standard items of 1mg respectively, with 10 mL acetone solution constant volumes, obtained concentration is the standard solution of 100 mg/L; Pipette each standard solution of 1.00 mL respectively, mix and use acetone stepwise dilution, obtained each concentration list mark and each concentration mixed standard solution.
3. high efficiency extraction according to claim 2 and liquid phase measure the method for carotenoid in yellow meat Peach fruits, it is characterized in that: the step extracting carotenoid preparation sample solution in its step b from pulp is: take pulp, according to solid-liquid ratio 1:6(g/mL) add acetone, lucifuge ultrasonic assistant extracts 1h, centrifugal 10 min of 10000 rpm at 4 DEG C, get supernatant and namely obtain sample solution, filter through the organic filter of 0.22 mm.
4. the high efficiency extraction according to any one of claim 1-3 and liquid phase measure the method for carotenoid in yellow meat Peach fruits, it is characterized in that: the carotenoid that drawing standard solution uses comprises xenthophylls, luteole, beta-cryptoxanthin, alpha-carotene, beta carotene.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105717209A (en) * | 2016-01-29 | 2016-06-29 | 甘肃农业大学 | Method for extracting carotenoid components in pepper leaves and quantitatively detecting content of carotenoid components |
| CN107144651A (en) * | 2017-05-05 | 2017-09-08 | 华中农业大学 | Arctic Sea fuchsin coccus B7740 produces the Structural Identification method of isoprenoid |
| CN107561171A (en) * | 2017-07-06 | 2018-01-09 | 北京林业大学 | The high efficiency extraction and assay method of carotenoid in Chinese rose petal |
| CN108680683A (en) * | 2018-04-17 | 2018-10-19 | 中国农业科学院郑州果树研究所 | Liquid chromatography detects the HPLC methods that carotenoid detects in the mobile phase formula and fruit of carotenoid in fruit |
| CN112557544A (en) * | 2020-12-09 | 2021-03-26 | 宁夏农林科学院枸杞科学研究所 | Method for detecting lycium carotenoid metabolites |
| CN113916995A (en) * | 2020-07-07 | 2022-01-11 | 内蒙古伊利实业集团股份有限公司 | Method for measuring zeaxanthin content in zeaxanthin raw material |
| CN115078590A (en) * | 2022-07-16 | 2022-09-20 | 福建省产品质量检验研究院(福建省缺陷产品召回技术中心) | Detection method for determining 8 carotenoid species by two-dimensional liquid chromatography |
| US11460451B2 (en) | 2019-12-18 | 2022-10-04 | Xi'an Jiaotong University | Method for simultaneously determining fat-soluble vitamins and carotenoids in serum |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040166199A1 (en) * | 2001-05-24 | 2004-08-26 | Agricultural Research Organization, The Volcani Center | Increasing bioavailability of carotenoids |
| CN102507775A (en) * | 2011-10-31 | 2012-06-20 | 广东东泰乳业有限公司 | Method for detecting content of xanthophyll in soy-based food |
| CN104090061A (en) * | 2014-07-30 | 2014-10-08 | 江苏省农业科学院 | Method for detecting mono-cis/bicis-isomer of zeaxanthin |
-
2015
- 2015-04-21 CN CN201510189708.0A patent/CN104749293A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040166199A1 (en) * | 2001-05-24 | 2004-08-26 | Agricultural Research Organization, The Volcani Center | Increasing bioavailability of carotenoids |
| CN102507775A (en) * | 2011-10-31 | 2012-06-20 | 广东东泰乳业有限公司 | Method for detecting content of xanthophyll in soy-based food |
| CN104090061A (en) * | 2014-07-30 | 2014-10-08 | 江苏省农业科学院 | Method for detecting mono-cis/bicis-isomer of zeaxanthin |
Non-Patent Citations (4)
| Title |
|---|
| DANIELE GIUFFRIDA ET AL.: "Determination of the carotenoid profile in peach fruits,juice and jam", 《FRUITS》 * |
| DAVID J.HART ET AL.: "Development and evaluation of an HPLC method for the analysis of carotenoids in foods, and the measurement of the carotenoid content of vegetables and fruits commonly consumed in the UK", 《FOOD CHEMISTRY》 * |
| 赵丰丽 等: "柿子皮类胡萝卜素的超声提取及抗氧化活性的研究", 《食品与发酵工业》 * |
| 颜少宾 等: "桃果实类胡萝卜素测定方法的研究", 《果树学报》 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105717209A (en) * | 2016-01-29 | 2016-06-29 | 甘肃农业大学 | Method for extracting carotenoid components in pepper leaves and quantitatively detecting content of carotenoid components |
| CN107144651A (en) * | 2017-05-05 | 2017-09-08 | 华中农业大学 | Arctic Sea fuchsin coccus B7740 produces the Structural Identification method of isoprenoid |
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| CN108680683A (en) * | 2018-04-17 | 2018-10-19 | 中国农业科学院郑州果树研究所 | Liquid chromatography detects the HPLC methods that carotenoid detects in the mobile phase formula and fruit of carotenoid in fruit |
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| CN112557544A (en) * | 2020-12-09 | 2021-03-26 | 宁夏农林科学院枸杞科学研究所 | Method for detecting lycium carotenoid metabolites |
| CN115078590A (en) * | 2022-07-16 | 2022-09-20 | 福建省产品质量检验研究院(福建省缺陷产品召回技术中心) | Detection method for determining 8 carotenoid species by two-dimensional liquid chromatography |
| CN115078590B (en) * | 2022-07-16 | 2023-08-04 | 福建省产品质量检验研究院(福建省缺陷产品召回技术中心) | Detection method for measuring 8 carotenoid types by two-dimensional liquid chromatography |
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