CN104739473A - Nanofiber yarn nerve conduit and preparing method thereof - Google Patents
Nanofiber yarn nerve conduit and preparing method thereof Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B17/00—Surgical instruments, devices or methods, e.g. tourniquets
- A61B17/11—Surgical instruments, devices or methods, e.g. tourniquets for performing anastomosis; Buttons for anastomosis
- A61B17/1128—Surgical instruments, devices or methods, e.g. tourniquets for performing anastomosis; Buttons for anastomosis of nerves
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61F—FILTERS IMPLANTABLE INTO BLOOD VESSELS; PROSTHESES; DEVICES PROVIDING PATENCY TO, OR PREVENTING COLLAPSING OF, TUBULAR STRUCTURES OF THE BODY, e.g. STENTS; ORTHOPAEDIC, NURSING OR CONTRACEPTIVE DEVICES; FOMENTATION; TREATMENT OR PROTECTION OF EYES OR EARS; BANDAGES, DRESSINGS OR ABSORBENT PADS; FIRST-AID KITS
- A61F2/00—Filters implantable into blood vessels; Prostheses, i.e. artificial substitutes or replacements for parts of the body; Appliances for connecting them with the body; Devices providing patency to, or preventing collapsing of, tubular structures of the body, e.g. stents
- A61F2/02—Prostheses implantable into the body
- A61F2/04—Hollow or tubular parts of organs, e.g. bladders, tracheae, bronchi or bile ducts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/18—Macromolecular materials obtained otherwise than by reactions only involving carbon-to-carbon unsaturated bonds
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/222—Gelatin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
- A61L27/14—Macromolecular materials
- A61L27/22—Polypeptides or derivatives thereof, e.g. degradation products
- A61L27/24—Collagen
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- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
- D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
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Abstract
The invention relates to a nanofiber yarn nerve conduit and a preparing method thereof. The nerve conduit is composed of a core layer and a shell layer. The core layer is made of nanofiber yarn, and the shell layer is made of a tubular nanofiber membrane. The preparing method comprises the steps that the nanofiber yarn is prepared through the electrospinning technique, and nanofiber in the yarn is arranged in parallel in the axial direction of the yarn; the nanofiber yarn is arranged in parallel and wrapped by a layer of nanofiber membrane, and the nerve conduit is formed. Operation is simple, stability is high, environmental friendliness and safety are achieved, the prepared nerve conduit is good in biocompatibility, adhering, spreading and proliferating of nerve cells can be promoted, the oriented growth of the nerve cells is promoted through the parallel nanofiber on the surface of the nanofiber yarn, and conduits of different sizes can be customized according to actual situations to meet the requirement for peripheral nerve regeneration.
Description
Technical field
The invention belongs to organizational project and biological manufactured materials and preparation field, particularly a kind of nano-fibre yams nerve trachea and preparation method thereof.
Background technology
Peripheral nerve injury is damage common clinically, and it is extensively present in traffic accident, industrial injury and daily life.In China, peripheral nerve injury case is newly-increased 60-90 ten thousand example every year, and the case wherein damaged by neural transplantation reparation is 30-50 ten thousand example.After peripheral nerve injury, carry out by autotransplantation the golden standard that bridge joint is current clinical treatment, but there is many shortcomings in the method, such as neural size do not mated, operation, graft area afunction, neuromatous generation etc. again.The reparation of peripheral nerve injury is still clinical a great problem, along with the development of tissue engineering technique and new material, the nerve trachea that various biomaterial builds is applied to repairing peripheral nerve injury, and application organizational project technique construction nerve trachea has very large development potentiality as the succedaneum of nerve autograft.By nerve trachea bridge joint injured nerve, namely utilize biomaterial to make the tubulose bridge joint damaged peripheral nerves broken ends of fractured bone, the regeneration for nervous tissue provides space and support.Because nerve trachea can provide a clog-free passage by neuranagenesis, can prevent the intrusion of surrounding tissue cells and interference from forming cicatrix.
Tissue engineering technique is utilized to repair neurologic defect, namely obtain to cultivate at tissue engineering bracket after a small amount of seed neurocyte or stem cell cultivate propagation in vitro in patient body and form complex, after being implanted into damaged part, seed cell continues propagation and forms one with peripheral nerve tissue, and repair deficiency is neural.Period, timbering material is degraded gradually, finally reaches the reparation completely on biological significance.Wherein timbering material occupies an important position in tissue engineering technique.One of organizational project three elements are the preparation of three-dimensional stent material, and timbering material is played a supporting role, for cell provides the attachment sites of growth and the place of proliferation and differentiation.Meanwhile, timbering material and the three dimensional structure of introducing suitable construction promote cell to be differentiated to form tissue along required direction thus repair a key factor of wound.
Nano-fiber material is because can be widely used in preparing various organization engineering rack from bionic extracellular matrix structure, 2005, [the Stevens M M such as Stevens, 2005] write articles on Science, the surface area proposing nanoscale support larger is conducive to adsorbing more protein, can provide for the receptor on cell membrane and more stick site, the protein of absorption also exposes more how hidden stick site by changing conformation, thus is conducive to cell adhesion and growth.Thus the cytoskeleton prepared with nanofiber can the physical arrangement of bionical human inner cell's epimatrix, promotes the regeneration of tissue.The method preparing nano fiber scaffold at present mainly contain be separated, self assembly, electrostatic spinning etc.Wherein electrostatic spinning technique because it is easy, economy, the suitability be wide etc., and feature gets the attention.US Patent No. 20100234863 A1 discloses a kind of method utilizing electrostatic spinning technique to prepare chitosan-polycaprolactone nano-fiber nerve conduit, but natural material good biocompatibility bad mechanical property, synthetic material good mechanical performance and biocompatibility is not good enough, both advantages that this support combines, experimental result shows that the nano-fiber nerve conduit of two component is conducive to Growth of Cells, effectively can promote the regeneration of nervous tissue.US Patent No. 20120150205 A1 discloses a kind of method utilizing fibroin fiber to prepare nerve trachea, and this nerve trachea has good biocompatibility and mechanical performance.Research shows, oriented nanofibers can control the neuronic direction of growth by contact guidance, and accelerate the maturation of schwann cell, the neurocyte that oriented nanofibers is cultivated is all along machine direction orientation, aixs cylinder also extends along machine direction, is conducive to sprawling and moving of cell.US Patent No. 8652215 B2 discloses a kind of technology utilizing oriented nanofibers to prepare nerve trachea, high speed rotating is utilized to accept to prepare oriented nanofibers film, there is the fiber of more than 75% orientation within the scope of 20 °, fibrous membrane is cut into the band shape of 17mm × 1mm along fiber orientation directions, many nanofiber ribbon are inserted in nerve trachea, experimental result shows that oriented nanofibers band can promote adhesion and the migration of cell, and then promotes the regeneration of rat tibial nerve.Chinese patent CN 103127548 A discloses a kind of preparation method of the artificial nerve catheter promoting neurologic defect to repair, utilize electrostatic spinning technique that poly butyric valeric acid copolyesters is prepared into the electrospun fiber membrane aligned, multilayer tubular structures is obtained with folding by cutting, through zoopery, decorative layer Fibronectin in pipe, confirms that this artificial nerve catheter can realize the reparation of large scale neurologic defect.
Summary of the invention
Technical problem to be solved by this invention is to provide a kind of nano-fibre yams nerve trachea and preparation method thereof, the inventive method is easy and simple to handle, favorable repeatability, material has good biocompatibility, bionical artificial nerve catheter structure is similar to natural nerve, the nerve trachea of specific dimensions can be customized for patient, in neurologic defect reparation, have huge application potential.
A kind of nano-fibre yams nerve trachea of the present invention, described nerve trachea is made up of sandwich layer and shell, and its center core layer is nano-fibre yams, and shell is tubular nanometer fibrous membrane; Nerve trachea length is 10-100mm, and internal diameter is 0.5-10mm, and wall thickness is 0.02-2mm.
Described nano fibre yarn line density is 10-200 root/mm
2.
The preparation method of a kind of nano-fibre yams nerve trachea of the present invention, comprising:
(1) materials A is mixed with electrostatic spinning solution A, then carries out spinning with double needle spinning system and obtain nano-fibre yams;
(2) be axially parallel to outside metal bar by nano-fibre yams along metal bar, obtain the metal bar with nano-fibre yams, be then carried on motor, ground connection also rotates;
(3) material B is mixed with electrostatic spinning solution B, electrostatic spinning, with the metal bar Rotation of receiver with nano-fibre yams in step (2), one deck nano fibrous membrane is formed outside nano-fibre yams, after spinning terminates, remove metal bar, cutting, obtain nano-fibre yams nerve trachea.
Bioactie agent and/or conductive material is added, as nerve growth factor (NGF) in electrostatic spinning solution A in described step (1); Nano-fibre yams carries out finishing, and wherein finishing is one or more in dipping fibronectin, dipping collagen protein, plasma treatment modification.
In described step (1), double needle electrostatic spinning system comprises a set of malleation electrostatic spinning system, a set of negative pressure electrostatic spinning system, rotating metallic funnel, yarn winder.
The technological parameter that the middle double needle spinning system of described step (1) carries out spinning is: flow is 0.3 ~ 5mL/h, malleation spinning system voltage is+8 ~+25kV, negative pressure spinning system voltage is-8 ~-25kV, the distance of two syringe needles and metal funnel is 10 ~ 30cm, metal funnel rotating speed is 200 ~ 4000rpm, and the winding speed of yarn winder is 0.1 ~ 10m/min.
In described step (2), the selection of metal bar diameter is determined according to the diameter nerve of required reparation.
In described step (2), nano fibre yarn line length is 5 ~ 50cm, and metal bar diameter is 0.5 ~ 20mm; The speed of rotation is 5rpm ~ 50rpm.
In described step (3), electrostatic spinning process parameter is flow is 0.5 ~ 2mL/h, and spinning voltage is 10 ~ 20kV, and receiving range is 5 ~ 20cm, and spinning duration is 1 ~ 5h.
In described step (1), in materials A, step (3), material B is biomaterial of the same race or not of the same race; Wherein biomaterial is synthesizing polymeric material or/natural biologic material.
Described synthesizing polymeric material is one or more in polycaprolactone, polylactic acid, polyurethane; Natural biologic material is one or more in collagen, gelatin, fibroin.
In described step (1), the quality concentration expressed in percentage by volume of electrostatic spinning solution A is 5-15%, and solvent is the one in trifluoroethanol, hexafluoroisopropanol; In step (3), the quality concentration expressed in percentage by volume of electrostatic spinning solution B is 10-15%, and solvent is the one in hexafluoroisopropanol, trifluoroethanol.
The present invention adopts method nano-fibre yams combined with nanofiber conduit, prepare a kind of novel nerve trachea, utilize the height-oriented structure of nanofiber in yarn to promote sprawling and shifting of neurocyte, and then promote the regeneration of nervous tissue, repair peripheral nerve defection.
The existence of center core layer nano-fibre yams of the present invention can provide support for the growth of neurocyte in nerve trachea and guide, and promotes the Formation and regeneration of nervous tissue in conduit; This preparation method is simple to operate, and stability is high, green safety, nerve trachea good biocompatibility prepared by high financial profit, can have more practical situation adjust size structure to adapt to the needs of CO2 laser weld.
The present invention is simple to operate, stability is high, green safety, the nerve trachea good biocompatibility of preparation, the adhesion of neurocyte can be promoted, sprawl and breed, wherein the parallel nanofiber on nano-fibre yams surface is conducive to the oriented growth of neurocyte, and the conduit that can customize different size according to practical situation meets the needs that peripheral nerve is repaired.
beneficial effect
The invention has the advantages that and nano-fibre yams and nanofiber conduits join are prepared the nerve trachea with sandwich layer and shell structurre, the nano-fibre yams surface nanofibre of sandwich layer is along yarn axis to height-oriented, sprawling and moving of neurocyte can be guided, promote the regeneration of nervous tissue;
The inventive method is easy and simple to handle, favorable repeatability, material has good biocompatibility, bionical artificial nerve catheter structure is similar to natural nerve, can customize the nerve trachea of specific dimensions, in neurologic defect reparation, have huge application potential for patient.
Accompanying drawing explanation
Fig. 1 is the schematic diagram utilizing double needle electrostatic spinning system to prepare nano-fibre yams;
Fig. 2 is the schematic diagram utilizing nano-fibre yams to build nerve trachea;
Fig. 3 is SEM picture (a) of PLLA nano-fibre yams in embodiment 1, (b) side, (c) cross section and fiber diameter distribution profile (d);
Fig. 4 is the photo (b) after the photo (a) and Static Spinning one deck P (LLA-CL) nano fibrous membrane that in embodiment 1, PLLA nano-fibre yams arranges outside metal bar;
Fig. 5 is the sectional view of nerve trachea in embodiment 1; Wherein a is the SEM figure of nerve trachea complete cross section; B is the SEM figure of nerve trachea partial cross section;
Fig. 6 is the SEM picture of prepared nerve trachea and schwann cell Dual culture in embodiment 1; Incubation time a is 3 days, and b is 5 days, and c is 7 days, the SEM figure under 500 times; Incubation time d is 3 days, and e is 5 days, and f is 7 days, the SEM figure under 2000 times.
Detailed description of the invention
Below in conjunction with specific embodiment, set forth the present invention further.Should be understood that these embodiments are only not used in for illustration of the present invention to limit the scope of the invention.In addition should be understood that those skilled in the art can make various changes or modifications the present invention, and these equivalent form of values fall within the application's appended claims limited range equally after the content of having read the present invention's instruction.
Embodiment 1
(1) by 0.75g Poly-L-lactic acid (PLLA, molecular weight 500kDa) be dissolved in 10mL hexafluoroisopropanol solution, be mixed with the PLLA solution that mass volume ratio is 7.5%, this solution double needle spinning system is prepared PLLA nano-fibre yams, two syringe needle liquid inventories are all 1.0mL/h, malleation spinning system voltage is+12kV, negative pressure spinning system voltage is-12kV, the distance of two syringe needles and metal funnel is 15cm, metal funnel rotating speed is 500rpm, the winding speed of yarn winder is 0.5m/min, obtains PLLA nano-fibre yams;
(2) by PLLA nano-fibre yams along metal bar to be axially parallel to outside metal bar (Fig. 4 a), yarn effective length is 6cm, metal bar diameter is 2.5mm, metal bar with nano-fibre yams is loaded on an electric motor, make its ground connection and rotate, rotating speed is 5rpm;
(3) by 1.2g Poly-L-lactic acid caprolactone (P (LLA-CL), molecular weight 300kDa, LA:CL=50:50) be dissolved in 10mL hexafluoroisopropanol, be mixed with P (LLA-CL) solution that mass volume ratio is 12%, this solution is used for electrostatic spinning, liquid inventory is 1mL/h, spinning voltage is 12kV, accepting distance is 15cm, with the metal bar Rotation of receiver with nano-fibre yams, outside nano-fibre yams, form one deck P (LLA-CL) nano fibrous membrane (b of Fig. 4);
(4) electrostatic spinning 2h, after terminating, removes metal bar, cutting, and being namely able to PLLA nano-fibre yams is sandwich layer, and P (LLA-CL) nano fibrous membrane is the nerve trachea of shell, and catheter length is 6cm, internal diameter 2.5mm, wall thickness ~ 0.2mm.
This nerve trachea is cut to 1.5mm short tube and with schwann cell Dual culture, test the growth rate of schwann cell at the 3rd, 5,7 day observation of cell at the growing state (Fig. 6) of catheter interior.Can be observed, schwann cell well adheres on nano-fibre yams surface, and along yarn axis to stretching, extension orientation.Along with the increase of incubation time, cell quantity significantly increases, and shows that this support effectively can promote propagation and the migration of neurocyte.
Embodiment 2
(1) by 0.6g polycaprolactone (PCL, molecular weight 80kDa) be dissolved in 10mL trifluoroethanol solution with 0.6g pigskin gelatin, be mixed with the PCL/ gelatin solution that mass volume ratio is 12%, this solution double needle spinning system is prepared PCL/ gelatine nano fiber yarn, two syringe needle liquid inventories are all 1.2mL/h, malleation spinning system voltage is+10kV, negative pressure spinning system voltage is-10kV, the distance of two syringe needles and metal funnel is 15cm, metal funnel rotating speed is 300rpm, the winding speed of yarn winder is 0.3m/min, obtain PCL/ gelatine nano fiber yarn,
(2) PCL/ gelatine nano fiber yarn is axially parallel to outside metal bar along metal bar, yarn effective length is 10cm, and metal bar diameter is 2mm, is loaded on an electric motor by the metal bar with nano-fibre yams, make its ground connection and rotate, rotating speed is 5rpm;
(3) 1.2g P (LLA-CL) is dissolved in 10mL hexafluoroisopropanol, be mixed with P (LLA-CL) solution that mass volume ratio is 12%, this solution is used for electrostatic spinning, liquid inventory is 1mL/h, spinning voltage is 15kV, accepting distance is 15cm, with the metal bar Rotation of receiver with nano-fibre yams, forms one deck P (LLA-CL) nano fibrous membrane outside nano-fibre yams;
(4) electrostatic spinning 2h, after terminating, removes metal bar, cutting, and being namely able to PCL/ gelatine nano fiber yarn is sandwich layer, and P (LLA-CL) nano fibrous membrane is the nerve trachea of shell, and catheter length is 10cm, internal diameter 2.0mm, wall thickness ~ 0.2mm.
Embodiment 3
(1) by 0.75g Poly-L-lactic acid (PLLA, molecular weight 500kDa) be dissolved in 10mL hexafluoroisopropanol solution, be mixed with the PLLA solution that mass volume ratio is 7.5%, this solution double needle spinning system is prepared PLLA nano-fibre yams, two syringe needle liquid inventories are all 1.0mL/h, malleation spinning system voltage is+12kV, negative pressure spinning system voltage is-12kV, the distance of two syringe needles and metal funnel is 15cm, metal funnel rotating speed is 500rpm, the winding speed of yarn winder is 0.5m/min, obtain PLLA nano-fibre yams, subsequently, 0.2g collagen protein is dissolved in 10mL deionized water, obtain the collagen solution that mass volume ratio is 2%, obtained PLLA nano-fibre yams is infiltrated on 2h in collagen solution, take out afterwards and drying at room temperature, obtain the PLLA nano-fibre yams of surface-coated collagen protein.
(2) the PLLA nano-fibre yams that collagen protein is modified axially is parallel to outside metal bar along metal bar, yarn effective length is 4cm, metal bar diameter is 1.0mm, metal bar with nano-fibre yams is loaded on an electric motor, make its ground connection and rotate, rotating speed is 5rpm;
(3) by 1.2g P (LLA-CL), be dissolved in 10mL hexafluoroisopropanol, be mixed with P (LLA-CL) solution that mass volume ratio is 12%, this solution is used for electrostatic spinning, liquid inventory is 2mL/h, and spinning voltage is 10kV, and accepting distance is 10cm, with the metal bar Rotation of receiver with nano-fibre yams, outside nano-fibre yams, form one deck P (LLA-CL) nano fibrous membrane;
(4) electrostatic spinning 1h, after terminating, removes metal bar, cutting, the PLLA nano-fibre yams being namely able to collagen protein modification is sandwich layer, and P (LLA-CL) nano fibrous membrane is the nerve trachea of shell, catheter length is 4cm, internal diameter 1.0mm, wall thickness ~ 0.15mm.
Claims (11)
1. a nano-fibre yams nerve trachea, is characterized in that: described nerve trachea is made up of sandwich layer and shell, and its center core layer is nano-fibre yams, and shell is tubular nanometer fibrous membrane; Nerve trachea length is 10-100mm, and internal diameter is 0.5-10mm, and wall thickness is 0.02-2mm.
2. a kind of nano-fibre yams nerve trachea according to claim 1, is characterized in that: described nano fibre yarn line density is 10-200 root/mm
2.
3. a preparation method for nano-fibre yams nerve trachea as claimed in claim 1, comprising:
(1) materials A is mixed with electrostatic spinning solution A, then carries out spinning with double needle spinning system and obtain nano-fibre yams;
(2) be axially parallel to outside metal bar by nano-fibre yams along metal bar, obtain the metal bar with nano-fibre yams, be then carried on motor, ground connection also rotates;
(3) material B is mixed with electrostatic spinning solution B, electrostatic spinning, with the metal bar Rotation of receiver with nano-fibre yams in step (2), after spinning terminates, removes metal bar, cutting, obtain nano-fibre yams nerve trachea.
4. the preparation method of a kind of nano-fibre yams nerve trachea according to claim 3, is characterized in that: add bioactie agent and/or conductive material in electrostatic spinning solution A in described step (1); Nano-fibre yams carries out finishing, and wherein finishing is one or more in dipping fibronectin, dipping collagen protein, plasma treatment modification.
5. the preparation method of a kind of nano-fibre yams nerve trachea according to claim 3, is characterized in that: in described step (1), double needle electrostatic spinning system comprises a set of malleation electrostatic spinning system, a set of negative pressure electrostatic spinning system, rotating metallic funnel, yarn winder.
6. the preparation method of a kind of nano-fibre yams nerve trachea according to claim 3, it is characterized in that: the technological parameter that the middle double needle spinning system of described step (1) carries out spinning is: flow is 0.3 ~ 5mL/h, malleation spinning system voltage is+8 ~+25kV, negative pressure spinning system voltage is-8 ~-25kV, the distance of two syringe needles and metal funnel is 10 ~ 30cm, metal funnel rotating speed is 200 ~ 4000rpm, and the winding speed of yarn winder is 0.1 ~ 10m/min.
7. the preparation method of a kind of nano-fibre yams nerve trachea according to claim 3, is characterized in that: in described step (2), nano fibre yarn line length is 5 ~ 50cm, and metal bar diameter is 0.5 ~ 20mm; The speed of rotation is 5rpm ~ 50rpm.
8. the preparation method of a kind of nano-fibre yams nerve trachea according to claim 3, it is characterized in that: in described step (3), electrostatic spinning process parameter is: flow is 0.5 ~ 2mL/h, spinning voltage is 10 ~ 20kV, receiving range is 5 ~ 20cm, and spinning duration is 1 ~ 5h.
9. the preparation method of a kind of nano-fibre yams nerve trachea according to claim 3, is characterized in that: in described step (1), in materials A, step (3), material B is biomaterial of the same race or not of the same race; Wherein biomaterial is synthesizing polymeric material or/natural biologic material.
10. the preparation method of a kind of nano-fibre yams nerve trachea according to claim 9, is characterized in that: described synthesizing polymeric material is one or more in polycaprolactone, polylactic acid, polyurethane; Natural biologic material is one or more in collagen, gelatin, fibroin.
The preparation method of 11. a kind of nano-fibre yams nerve tracheas according to claim 3, it is characterized in that: in described step (1), the quality concentration expressed in percentage by volume of electrostatic spinning solution A is 5-15%, and solvent is the one in trifluoroethanol, hexafluoroisopropanol; In step (3), the quality concentration expressed in percentage by volume of electrostatic spinning solution B is 10-15%, and solvent is the one in hexafluoroisopropanol, trifluoroethanol.
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| CN108175874A (en) * | 2018-01-10 | 2018-06-19 | 深圳市第二人民医院 | Compound support frame material based on electrostatic spinning yarn and hydrogel and preparation method thereof |
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| CN111097069A (en) * | 2019-07-04 | 2020-05-05 | 南开大学 | Bionic degradable artificial nerve conduit for regulating immune microenvironment and guiding regeneration by using topological structure and preparation method thereof |
| CN112386739A (en) * | 2019-08-16 | 2021-02-23 | 南京理工大学 | Preparation method of high-conductivity nanofiber nerve conduit |
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