CN104718969A - Production technology and using method for edible mushroom branch mother seeds - Google Patents
Production technology and using method for edible mushroom branch mother seeds Download PDFInfo
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- CN104718969A CN104718969A CN201310696412.9A CN201310696412A CN104718969A CN 104718969 A CN104718969 A CN 104718969A CN 201310696412 A CN201310696412 A CN 201310696412A CN 104718969 A CN104718969 A CN 104718969A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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Abstract
The invention discloses a production technology and using method for edible mushroom branch mother seeds. Novel mother seeds and the expanding propagation of the second-class strain by the mother seeds are produced through the following production technologies which include: utilizing wooden branches, making the branches, preparing the nutrient solution, soaking the branches with the nutrient solution, preparing the culture medium, tabulating, sterilizing, inoculating and culturing. The edible mushroom branch mother seeds are unique in preparation method, simple in technology, low in cost, high in survival rate, large in propagation coefficient, low in pollution rate and neat and robust in hypha growth. Moreover, the growth speed of the mother seeds can be 10-15 days faster than the normal mother seeds after the second-class strain is inoculated.
Description
Technical field
The invention belongs to parent edible fungus kind production technical field, be specifically related to edible mushroom branch mother and plant production technology and using method.
Background technology
Generally adopt in parent edible fungus kind production process, agar medium is produced, and is applicable to multiple eating starter kind and produces.But it is short that agar medium mother plants the shelf-life, and be no more than one month under normal temperature, Cord blood can not more than 3 months.Survival rate is not very high, and reproduction coefficient is also little, and female a kind can only connect 6 to 8 bottles or bag secondary kind.Publication number for: CN1440636A, denomination of invention for: the application for a patent for invention of " produce edible fungi liquid strain needed for special mother kind ", technics comparing is complicated.Be only applicable to the production of batch production liquid spawn.Publication number CN1596586A, denomination of invention are guaranteed the quality also longer (4-6) moon for " edible fungus wood slag mother seed and preparation method thereof ", and reproduction coefficient is also large, and survival rate is also very high, but result of use is planted the same with agar medium mother.Publication number CN103329735A, denomination of invention are that " a kind of rice that utilizes makes the method for edible mushroom test tube stock as medium " connects the reproduction coefficient of second class inoculum also not quite, and survival rate is better, and result of use agar medium mother plants the same.
Summary of the invention
Object of the present invention be exactly provide for the deficiencies in the prior art that a kind of cost is low, survival rate is high, reproduction coefficient is large, long shelf-life, secondary kind grows fast branch mother and plants production technology after use.
The object of this invention is to provide that a kind of cost is low, survival rate is high, reproduction coefficient is large, long shelf-life, secondary kind comparatively uses and conventionally femalely plants the growth branch of fast 10 ~ 15 days mother and plant after use.
The object of this invention is to provide that a kind of cost is low, survival rate is high, reproduction coefficient is large, long shelf-life, secondary kind comparatively uses conventional female using method of planting the growth branch of fast 10 ~ 15 days mother and planting after use.
For achieving the above object, technical scheme of the present invention is.
Edible mushroom branch mother plants production technology and using method comprises the following steps.
(1) making of branch: make female branch of planting and will select diameter 0.2 ㎝ ~ 0.3 about ㎝, long 6 ~ 12 ㎝, the side in garden flat can, select cork relatively good, taking second place of hardwood and bamboo, professed machine can be selected to process, also can select wooden toothpick.
(2) nutrient solution is prepared: in 1000 ml waters, add 50 grams of soya-bean cakes, 20 grams of white sugar, 3 grams of potassium dihydrogen phosphates, 1.5 magnesium sulfate, above-mentioned each component is put in water and soaks 20 minutes, be heated to 100 DEG C 15 minutes, filter out soya-bean cake to supply evaporation moisture content to obtain nutrient solution for subsequent use, nutrient solution of the present invention suitable hypha of edible fungus also can be selected grow other fill a prescription.
(3) branch process: be first dipped in by branch in nutrient solution and soak 24 ~ 36 hours, also can heat nutrient solution and boil branch and inhale nutrient solution, and branch does not have the white heart just passable, and generally boil 2 ~ 3 hours, the branch handled well is for subsequent use.
(4) preparation of medium: produce wood decay fungi medium: select 81% thin wood chip, 15% wheat bran, 2% corn flour, 1% sugar, 1% gypsum, water content 60 ~ 65%; Produce the medium of straw rotting fungus: rice straw powder 50%, cow dung powder 38%, corn flour 4%, analysis for soybean powder 3%, superphosphate 2%, magnesium sulfate 1%, calcium carbonate 2%, water content 58 ~ 60%; Stir according to a conventional method for subsequent use.
(5) tubulature: select long 100 ~ 160 ㎜ test tubes according to the length of branch, shorter than the test tube doing agar medium by 30 ~ 40%, price is much lower.The branch handled well with nutrient solution and the medium configured are mixed, make branch is stained with medium, at the bottom of pipe, put a small amount of medium then load branch, a small amount of medium is being put at the mouth of pipe, medium is made to be filled in the space of branch, branch is put a little medium just passable, flatten medium and be stoppered tampon.(test tube of the present invention also can use other containers).
(6) sterilizing: will be stoppered test tube sterilizing in pressure cooker of tampon, temperature 121 ~ 127 DEG C, the time is 60 ~ 90 minutes.Also can normal-pressure sterilization temperature 100 DEG C, 8 hours time.
(7) inoculate: after sterilizing terminates, taking out test tube connecing the cooling of bacterium Indoor Natural, treating below pipe temperature drop to 30 DEG C, just can aseptically, inoculating by sterile working code.
(8) constant temperature culture: postvaccinal strain tube is put into constant incubator or culturing room, constant temperature culture at temperature control 25 DEG C, generally also covered with test tube (the different growth rate of kind is different) at 10 ~ 15 days, and the growth rate that common mother plants is similar.
(9) quality inspection packaging: after tree fungus covers with pipe, just can carry out the qualified bacterial classification of quality examination and pack for sale or use the production carrying out second class inoculum.
The using method of the female bacterium of branch: each branch can connect a second class inoculum, one is the seal closure using tree fungus special, tree fungus inserts in the cultivation of bacterium bag just passable by connecing tube, two, if do not used this special cultivation lid, then can making one by oneself, thinner to connect tube just passable, select the glass tube of long 4 ~ 6 ㎝ of diameter 6 ~ 10 ㎜ or resistant to elevated temperatures the first day of the lunar month material Guan Junke, one of this pipe is allowed to insert medium 0.3 about ㎝, bacterium bag and connect tube leather sheath or cord is tightened, mouth of pipe tampon seals, aseptically tampon is pulled out after sterilizing cooling, the female bacterium of a branch is inserted just passable in inoculated tube, tree fungus must insert in medium, above with medium maintain an equal level or slightly high can, this homemade tiny advantage connecing tube is used to be 1, the mouth of pipe tiny inoculation time air the little probability reducing pollution of amount of flow, 2, during the tiny inoculation of the mouth of pipe, the sudden and violent leakage face of medium is little also reduces pollution, 3, due to pollution can be also reduced by the charge level covered with very soon in pipe after bacterial classification sprouting after the tiny inoculation of the mouth of pipe.
Compared with prior art, tool of the present invention has the following advantages:.
1, the preparation method of branch mother kind of the present invention is unique, does not need special equipment, easily implements.
2, enforcement of the present invention, replaces long test tube with short test tube, the space that cost-saving minimizing takies; Replacing expensive agar to make mother culture media with branch, reduce cost to a great extent again, is a kind of not only economy but also practical technology.
3, present invention process is simple, easily grasps, i.e. saving of labor but also save time; Agar medium packing test tube will complete within the regular hour, and complete before and will solidifying after agar melts, the present invention is then unrestricted; Will put inclined-plane after agar medium sterilizing, will complete within a certain period of time yet, the present invention does not then have this step, and directly cooling inoculation is just passable.
4, mother's kind survival rate of the present invention is high, sprout fast, because mycelial growth is inner to branch, in seeded process, only have outside mycelia to come to harm inner mycelia by less than injury, situation about therefore invariably sprouting after inoculation, and more conventional mother plant sprout fast, because inner mycelia does not come to harm, there is no recovery process, with regard to direct growth, and mycelial growth is neatly healthy and strong.
5, branch mother kind reproduction coefficient of the present invention is large, test tube thick equally as diameter 20 ㎜, common mother plant connect second class inoculum also with regard to 6 ~ 8 bottles or bag, and branch mother plants often pipe dress 35 ~ 50, branch (branch thickness different), each branch connects a secondary kind, switchable 35 ~ 50 secondary kinds.
6, branch of the present invention mother plants long shelf-life, and normal temperature can 3 months, and low temperature can reach 9 months, and does not affect result of use.
7, comparatively than using conventional female second class inoculum growth of planting to cover with for fast 10 ~ 15 days after branch mother of the present invention plants inoculation second class inoculum, because using the female bacterium of branch, inoculation just extend into 6 ~ 12 ㎝ in medium, grows together after sprouting in medium, so the growth rate of secondary bacterium is just fast.
8, use branch mother to plant and produce secondary kind, connect fungi pollution rate low, use branch mother to plant and connect bacterium by method of the present invention and can reduce the pollution rate producing secondary bacterium greatly.
Embodiment
Below in conjunction with embodiment, the present invention is further illustrated, but this example does not produce any restriction to the present invention.
1, the production technology that the branch mother being raw material with wooden toothpick plants and using method, embodiment is as follows:.
2, select diameter 0.2 about ㎝, long 6,5 ㎝, wooden toothpick, selects toothpick specially to process cost-saving, and one, wooden toothpick is sharp, is also convenient to insert in medium, easy to use.
3, prepare nutrient solution: in 1000 ml waters add 50 can soya-bean cake, 20 grams of white sugar, 3 grams of potassium dihydrogen phosphates, 1.5 magnesium sulfate, above-mentioned each component is put in water and soaks 20 minutes, be heated to 100 DEG C 15 minutes, filter out soya-bean cake and supply evaporation water to get nutrient solution for subsequent use.
4, toothpick process: be first dipped in by toothpick in nutrient solution and soak 24 hours, toothpick does not have the white heart just passable, and the branch handled well is for subsequent use.
5, the preparation of medium: planting according to the mother that will make is will produce wood decay fungi or straw rotting fungus and determine the medium selected, and this example selects wood decay fungi medium: select 81% thin wood chip, 15% wheat bran, 2% corn flour, 1% sugared, 1% gypsum water content 60 ~ 65%; Stir according to a conventional method for subsequent use.
6, tubulature: the test tube selecting 20 × 110 ㎜ specifications, the toothpick handled well with nutrient solution and the medium configured are mixed, make on toothpick, be stained with medium, at the bottom of pipe, put a small amount of medium then load toothpick, point at the bottom of pipe, put into a small amount of medium again at the mouth of pipe, medium is made to be filled in the space of toothpick, toothpick is put a little medium just passable, flatten medium and be stoppered tampon, often pipe dress about 45 ~ 50, toothpick.
7, sterilizing: will be stoppered test tube sterilizing in pressure cooker of tampon, temperature 127 DEG C, the time is 60 minutes.
8, inoculate: after sterilizing terminates, taking out test tube connecing the cooling of bacterium Indoor Natural, treating below pipe temperature drop to 30 DEG C, just can aseptically, inoculating by sterile working code, accessing woodear bacterial classification.
9, constant temperature culture: Jie Zhong ?strain tube after auricularia auriculajudae put into constant incubator or culturing room, constant temperature culture at temperature control 25 DEG C, generally also covers with test tube 15 ~ 17.
The method of woodear secondary kind of 10, transferring: second class inoculum can be sawdust medium also can be branch, pack by the operational procedure process composts or fertilisers of cultivating of secondary kind, homemade tiny inoculated tube is selected in sealing, with the first day of the lunar month material pipe of long 5 ㎝ of diameter 8 ㎜, one is inserted medium 0.2 about ㎜, sack is drawn close to pipe and is tightened with leather sheath, beyond the Great Wall the sterilizing of tampon conventional method.Be cooled to less than 30 DEG C after sterilizing, aseptically by the inoculation of sterile working code, during inoculation, the female bacterium of toothpick must insert in the space of the inner or branch of medium by inoculated tube, top and charge level or branch head flattens or bacterial classification is slightly high.Pollute low by this method inoculation, use the secondary kind fast growth that this mother plants.
Claims (8)
1. edible mushroom branch mother plants production technology and using method, its feature utilizes wooden branch to pass through, and the immersion of the making of branch, the preparation of nutrient solution, branch nutrient solution, the preparation of medium, tubulature, sterilizing, connects the production technology such as bacterium, cultivation and a kind of novel mother that produces plants and this mother plants and expands the using method of numerous second class inoculum.
2. edible mushroom branch according to claim 1 mother plants production technology and using method, and the culture that the mother of making plants use is wooden or the branch of bamboo.
3. plant production technology and using method according to claim 1 and edible mushroom branch according to claim 2 mother, the branch making the female kind of cultivation will be dipped in nutrient solution and soak 24 ~ 36 hours, also can heat nutrient solution to boil branch and inhale nutrient solution, branch does not have the white heart just passable, general 2 ~ 3 hours.
4. plant production technology and using method according to claim 1 and edible mushroom branch according to claim 3 mother, the nutrient solution prescription selected is: add 50 grams of soya-bean cakes, 20 grams of white sugar, 3 grams of potassium dihydrogen phosphates, 1.5 magnesium sulfate in 1000 ml waters, the nutrient solution of other formulas that nutrient solution of the present invention also can select suitable hypha of edible fungus to grow.
5. plant production technology and using method according to claim 1 and edible mushroom branch according to claim 2 mother, need to configure the solid culture medium used together with branch, medium is divided into wood decay fungi medium and straw rotting fungus medium, and the present invention also can use other culture medium prescriptions of other suitable wood decay fungi and straw rotting fungus growth on producing.
6. plant production technology and using method according to claim 1 and edible mushroom branch according to claim 5 mother, the medium mixing that branch raw material is wanted and prepared, make branch is stained with medium, and at the bottom of pipe, put a small amount of medium, then load branch, putting into a small amount of medium at the mouth of pipe, make medium be filled in the space of branch, branch is put a little medium just passable, flatten medium and be stoppered tampon.
7. edible mushroom branch mother according to claim 1 plants production technology and using method, and production uses test tube that other containers also can be used for culture.
8. edible mushroom branch mother according to claim 1 plants production technology and using method, the using method that the mother produced plants can select tree fungus special-purpose sealing lid or self-control one thinner to connect tube just passable, select the glass tube of long 4 ~ 6 ㎝ of diameter 6 ~ 10 ㎜ or resistant to elevated temperatures the first day of the lunar month material Guan Junke, one of this pipe is allowed to insert medium 0.3 about ㎝, bacterium bag and connect tube leather sheath or cord is tightened, mouth of pipe tampon seals, aseptically tampon is pulled out after sterilizing cooling, the female bacterium of a branch is inserted just passable in inoculated tube, tree fungus must insert in medium, above with medium maintain an equal level or slightly high can.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201310696412.9A CN104718969A (en) | 2013-12-18 | 2013-12-18 | Production technology and using method for edible mushroom branch mother seeds |
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| CN201310696412.9A CN104718969A (en) | 2013-12-18 | 2013-12-18 | Production technology and using method for edible mushroom branch mother seeds |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105237139A (en) * | 2015-08-29 | 2016-01-13 | 安远县天华现代农业有限责任公司 | Preparation and culturing method of tree branch bacterial strain of pleurotus eryngii and hypsizygus marmoreus |
| CN105347885A (en) * | 2015-11-12 | 2016-02-24 | 吴长发 | Bottled wood chip bacterium culture medium |
| CN105543101A (en) * | 2015-10-11 | 2016-05-04 | 福建省中延菌菇业有限公司 | Antrodia camphorate mother strain preparation method |
| CN108739047A (en) * | 2018-05-10 | 2018-11-06 | 山东恒发食用菌有限公司 | A kind of production of Pleurotus nebrodensis tree fungus and breeding method |
| CN111448946A (en) * | 2020-06-01 | 2020-07-28 | 安徽省百麓现代农业科技有限公司 | Dictyophora rubrovalvata branch strain cultivation method |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105237139A (en) * | 2015-08-29 | 2016-01-13 | 安远县天华现代农业有限责任公司 | Preparation and culturing method of tree branch bacterial strain of pleurotus eryngii and hypsizygus marmoreus |
| CN105543101A (en) * | 2015-10-11 | 2016-05-04 | 福建省中延菌菇业有限公司 | Antrodia camphorate mother strain preparation method |
| CN105543101B (en) * | 2015-10-11 | 2018-09-04 | 福建省中延菌菇业有限公司 | A kind of Antrodia camphorata parent species preparation method |
| CN105347885A (en) * | 2015-11-12 | 2016-02-24 | 吴长发 | Bottled wood chip bacterium culture medium |
| CN108739047A (en) * | 2018-05-10 | 2018-11-06 | 山东恒发食用菌有限公司 | A kind of production of Pleurotus nebrodensis tree fungus and breeding method |
| CN111448946A (en) * | 2020-06-01 | 2020-07-28 | 安徽省百麓现代农业科技有限公司 | Dictyophora rubrovalvata branch strain cultivation method |
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