CN104713816B - A kind of whole blood CRP detection means and method and a kind of blood cell analyzer - Google Patents
A kind of whole blood CRP detection means and method and a kind of blood cell analyzer Download PDFInfo
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- CN104713816B CN104713816B CN201510059624.5A CN201510059624A CN104713816B CN 104713816 B CN104713816 B CN 104713816B CN 201510059624 A CN201510059624 A CN 201510059624A CN 104713816 B CN104713816 B CN 104713816B
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Abstract
The invention discloses a kind of whole blood CRP detection means and method and a kind of blood cell analyzer, including syringe unit, dilution allocation unit, DIFF channel measurements unit, reagent unit, counting cell unit, sampling needle, negative pressure chamber and discharging of waste liquid unit；Reagent unit includes dilution, WBC reagents, DIFF reagents and the 2nd CRP reagents；Counting cell unit includes CRP ponds, DIFF ponds, WBC ponds, RBC ponds, the reaction and the reaction of RBC channel samples and reagent of reaction, the reaction of DIFF channel samples and reagent, WBC channel samples and reagent for completing CRP channel samples and reagent.The cellanalyzer of the classification blood routines of CRP and five can be simultaneously detected using the present invention, each sense channel parallel detection, test speed is fast.
Description
Technical field
The present invention relates to blood testing field, more particularly to a kind of fluid system with whole blood CRP detections and detection
Method, and using the blood cell analyzer of the system and method.
Background technology
CRP (C-reaction protein, c reactive protein) is that body is subject to the inflammation such as microorganism invasion or tissue damage
The Acute phase protein of liver cell synthesis during sexual stimulus, the ring-type pentamer formed by non-covalent bond by five identical subunits,
This characteristic structural makes it range five poly- elements the calbindin of immune defense characteristic (one group have) family.CRP is machine
A part for body nonspecific immunity mechanism, can activating complement classical pathway, strengthen leucocyte phagocytosis, adjust lymph
Cell or monokaryon/macrophage systemic-function, promote the generation of macrophage tissue factor, can also be examined in atherosclerotic plaque
Measure CRP.CRP just quick risings, just reach peak value in 48 hours after inflammation starts a few hours, disappear with lesion, tissue and
The recovery CRP of function is accordingly down to normal level.The characteristics of CRP, determines that its testing result has extremely important clinic in hospital
Value.
Current CRP detections mainly have two ways：One kind is to detect CRP in Biochemical Analyzer, and blood sample is first centrifuged, and is taken out
Serum, reaction tank is added by serum, reaction reagent, and CRP contents are measured by transmittance purifying method, about 90 seconds testing times, is somebody's turn to do
Method is adapted to the hospital of large sample amount；Another kind is individually to detect CRP using specific protein analyzer, by hand will with quantitative appliances
Blood sample, reaction reagent add reaction tank, and CRP contents are measured by scattering turbidimetry method, and the testing time is most fast 90 seconds, the method behaviour
Make cumbersome, be adapted to outpatient service and emergency department.
For large hospital, due to being all equipped with Biochemical Analyzer, therefore in large hospital CRP and other biochemical class ginsengs
Number is detected in Biochemical Analyzer together.For be not equipped with Biochemical Analyzer small hospital and part Medium Sized Hospitals for
Emergency treatment requirement, typically carries out CRP detections by specific protein analyzer.
Many hospitals need to examine with two parameters of CRP with reference to WBC (white blood cell, leucocyte) with as clinic at present
Disconnected reference, though Biochemical Analyzer can survey CRP and can not survey WBC, specific protein analyzer can only survey CRP and can not survey WBC, therefore
There is a problem of can not be while measure WBC and CRP in same blood sample；Simultaneously for the demand of urgent diagnosis, need quickly, easily
Measure CRP to be used with as clinical diagnosis, existing specific protein analyzer needs sample-adding by hand, cumbersome, and Biochemical Analyzer is general
Suitable great amount of samples is analyzed together.
The problem of this method is：Hospitals at Present needs to do clinical judgment with reference to WBC and CRP, and major part instrument at present
The blood routine parameters such as WBC can only be surveyed or CRP can only be surveyed, when especially specific protein analyzer surveys CRP, need manual operations, operated
It is cumbersome not convenient, dumb.
There was only few measuring appratus at present can simultaneously survey five classification blood routine parameters and CRP, but its test speed is slow, uncomfortable
Close Clinical practice.
In addition, the A of Patent publication No CN 103336130 disclose a kind of whole blood immunity analytical equipment and use this device
Blood analyser, its implementation is：HGB (hemoglobin, erythrocyte) measurements are carried out in one reaction pool to be surveyed with CRP
Amount, the reaction tank needs individually to heat and temperature conditioning unit carries out temperature control to it.HGB measurements use scattered light urbidmetry, CRP to survey
Amount uses turbidimetry.HGB is measured with CRP measurements using with portion whole blood, whole blood is added into reaction tank by sampling needle, so
Reaction reagent is separately added into by reaction tank by membrane pump afterwards, irradiation light is shining into by reaction tank by radiation source after having reacted,
In different angular collection transmitted light intensities and scattered light intensity, HGB and CRP values are then calculated according to transmitted light intensity and scattered light intensity.
Barrier film constant displacement pump power source comes from air pressure, it is therefore desirable to which source of the gas and air-channel system are controlled to barrier film constant displacement pump,
When the logical negative pressure of the quantitative pumping chamber of barrier film, barrier film constant displacement pump sucks reagent from reagent bottle, when the logical malleation of the quantitative pumping chamber of barrier film,
Reagent is squeezed into reaction tank by barrier film constant displacement pump.
Although the above method quick and easy can test out HGB and CRP, its test system for providing need source of the gas and
Air-channel system.The method not only system complex, high cost, and source of the gas short life, need to change once for 1 year.
It is extensive and the above method needs to take out CRP reagents from refrigerator first when specific protein analyzer test CRP is carried out
Tested again after multiple normal temperature.After having tested and CRP reagents need to be put back into refrigerator and carry out refrigeration prevents reagent from going bad.Operation is compared
Trouble is not easy.
Therefore, prior art has yet to be improved and developed.
The content of the invention
Aim to solve the problem that existing method operation is numerous it is an object of the invention to provide a kind of whole blood CRP detection means and method
The problem of trivial, not convenient, system complex and high cost.
Technical scheme is as follows：
A kind of whole blood CRP detection means, it include syringe unit, dilution allocation unit, DIFF channel measurements unit,
Reagent unit, counting cell unit, sampling needle, negative pressure chamber and discharging of waste liquid unit, the syringe unit connection dilution distribution
Unit, reagent unit, counting cell unit, sampling needle and DIFF channel measurement units, for the distribution of reagent and dilution；Dilution
The whole machine that liquid allocation unit is used for dilution is distributed；The DIFF channel measurements unit is by LASER Light Source, photelectric receiver and stream
Dynamic room composition, completes the measurement of DIFF passages；The reagent unit includes dilution, WBC reagents, a DIFF reagents, second
DIFF reagents, a CRP reagents and the 2nd CRP reagents；The counting cell unit includes CRP ponds, DIFF ponds, WBC ponds, RBC ponds,
Reaction, the reaction of DIFF channel samples and reagent, WBC channel samples and reagent for completing CRP channel samples and reagent
Reaction and the reaction of RBC channel samples and reagent；The sampling needle is used to complete the absorption and distribution of blood sample；The negative pressure chamber
For being that WBC ponds and RBC ponds provide negative pressure；The waste liquid that the discharging of waste liquid unit is used to produce in whole machine is discharged outside machine.
Described whole blood CRP detection means, its described syringe unit is comprising the first syringe module, the second note
Emitter module, the 3rd syringe module and the 4th syringe module, the first syringe module drag three syringes for one, by one
Three syringe tubes composition that individual motor drives, three syringe tubes of the first syringe module are respectively intended to distribute WBC reagents
With DIFF reagents；The second syringe module is one-to-one syringe, and the syringe tube group put is driven by a motor
Into a syringe tube of the second syringe module is allocated dilution for connecting dilution allocation unit；Described 3rd
Syringe module is one drag two syringe, and two syringe tubes driven by a motor are constituted, the two of the 3rd syringe module
Individual syringe tube is respectively intended to connection sampling needle to carry out blood sampling point blood and will push away sample and flow into DIFF channel measurement units to be surveyed
Amount；The 4th syringe module drags three syringes for one, and three injection-tubes driven by a motor are constituted, the 4th syringe
Three syringe tubes of module are respectively intended to push away sheath stream parcel sample flow and carry out DIFF passage surveys into DIFF channel measurements unit
Amount and distribution CRP reagents.
Described whole blood CRP detection means, its described DIFF channel measurements unit include LASER Light Source, photelectric receiver and
Flow chamber, under the collective effect of the 3rd syringe module and the 4th syringe module, sample flow is wrapped by detection zone, complete
Into the measurement of DIFF passages.
Described whole blood CRP detection means, its described WBC pond, RBC ponds and negative pressure chamber composition impedance and HGB channel measurements
There are HGB measuring units on unit, wherein WBC ponds, HGB measuring units are made up of LED and photelectric receiver, will by LED
Blood sample of the light irradiation after through WBC reagent reactings, the light intensity for then being received according to photelectric receiver calculates HGB, wherein institute
WBC reagents are stated to be added to from the first syringe in WBC ponds by the first triple valve, after negative pressure chamber sets up negative pressure, WBC ponds and
Cell in RBC ponds flows to negative pressure chamber under the driving of negative pressure, and flow process each cell is counted, and completes WBC, RBC cell
Measurement.
Described whole blood CRP detection means, its described sampling needle completes blood sample in the presence of the 3rd syringe module
Draw and distribute.
Described whole blood CRP detection means, its described CRP pond is CRP measuring units, and it includes LASER Light Source, a CRP
Reagent adds mouth, the 2nd CRP reagents to add mouth, dilution to add mouth, CRP ponds matrix and photelectric receiver, a CRP reagents and
2nd CRP reagents are reacted after the 4th triple valve and the 5th triple valve are added to blood sample respectively, the LASER Light Source and
Photelectric receiver is arranged on the side wall of CRP ponds matrix, and LASER Light Source is launched laser and is radiated on the solution of reaction, in photoelectricity
LASER Light Source is received on receiver and launches scattering light, CRP concentration can be calculated according to the scattered light intensity for receiving.
Described whole blood CRP detection means, it also includes a reagent refrigerating unit, a CRP reagents and the 2nd CRP
Reagent storage in the reagent refrigerating unit, the reagent refrigerating unit include cooling fan, cooling piece, conduction cooling matrix and on
Lid, is provided with cooling fan below cooling piece, and conduction cooling matrix is arranged on cooling piece, and surrounds multiple intervals, and described first
CRP reagents and the 2nd CRP reagents are placed in the interval that cooling piece and conduction cooling matrix are surrounded, and lid is provided with interval, whole
Individual refrigerating system control is at 2-8 DEG C.
Described whole blood CRP detection means, it also includes a preheating unit, for the pre- of CRP reagents and DIFF reagents
Heating, is separately added into CRP ponds and DIFF ponds 20 and is reacted after heating, a DIFF reagents and the 2nd DIFF reagents are distinguished
Reagent is added in DIFF ponds by the second triple valve and the 3rd triple valve, CRP reagents and DIFF reagents share a preheating
Unit, the preheating unit includes heating plate, heat conduction substrate, CRP reagents heat conducting pipe, CRP reagents heat conducting pipe, DIFF examinations
Agent heat conducting pipe and the 2nd DIFF reagent heat conducting pipes, wherein, heat conduction base 6 and each heat conducting pipe are metal heat-conducting material.
A kind of blood cell analyzer, it is provided with the whole blood CRP detection means described in above-mentioned any one.
A kind of whole blood CRP detection methods, it is characterised in that comprise the following steps：
Step S1：By sampling needle draw blood sample；
Step S2：Blood sample in sampling needle is divided into it is appropriate in CRP ponds, while by a CRP reagents and second
CRP reagents are reacted in adding CRP ponds by the 4th syringe module, and two kinds of CRP reagents enter before adding through preheating unit
Row reagent is preheated；
Step S3：Blood sample in sampling needle is divided into it is appropriate in DIFF ponds, while by a DIFF reagents and the
Two DIFF reagents add DIFF ponds to be reacted by the first syringe module, through preheating unit before two kinds of DIFF reagents heating
Carry out reagent preheating；
Step S4：Blood sample in sampling needle is divided into it is appropriate in WBC ponds, by after diluted, sampling needle
A certain amount of diluted sample is drawn from WBC ponds, is then carried out instead by the first syringe module a certain amount of WBC reagents of addition
Should；
Step S5：Sampling needle is moved into RBC ponds, the diluted sample drawn from WBC ponds is divided into；
Step S6：CRP detections, DIFF detections, WBC/HGB detections, RBC detections carry out parallel detection measurement, are measured
After export accordingly result, and each passage is completed to clean
Beneficial effects of the present invention：The system and method that the present invention is provided can use same branch whole blood, while measuring the blood such as WBC
Conventional parameter and CRP parameters, without manual operations, full-automation completes whole measurement process；Test speed is fast, one minute can be defeated
Go out all results；And system, without single source of the gas and air-channel system, simple system is compact, good reliability, low cost.And this is
System band cold storage plant and CRP reagent preheating devices in instrument, refrigerator is put back to after having tested without taking out CRP reagents manually,
And without wait, can at any time carry out CRP tests.
Brief description of the drawings
Fig. 1 is the module diagram of the whole blood CRP detection means that the present invention is provided.
Fig. 2 is CRP measuring unit structural representations in the device that the present invention is provided.
Fig. 3 is reagent refrigerating unit structural representation in the device that the present invention is provided.
Fig. 4 is preheating unit structural representation in the device that the present invention is provided.
Fig. 5 is the whole blood CRP detection method circulation figures that the present invention is provided.
Specific embodiment
To make the objects, technical solutions and advantages of the present invention clearer, clear and definite, develop simultaneously embodiment pair referring to the drawings
The present invention is further described.
Referring to Fig. 1, present invention offer is a kind of to can be used for the whole blood CRP detection means of blood cell analyzer, the device energy
Enough enable a blood analyser while measuring the blood routine parameters such as WBC and CRP parameters, it is full-automatic without manual operations
Complete whole measurement process；Test speed is fast, can export all results within one minute；And system is without single source of the gas and gas circuit system
System, simple system is compact.
The wherein described whole blood CRP detection means for setting blood cell analyzer is specifically included：Including syringe unit, dilute
Release liquid allocation unit 1, DIFF channel measurements unit 2, reagent unit, counting cell unit, sampling needle 3, negative pressure chamber 23 and waste liquid row
Put unit 24, the syringe unit connection dilution allocation unit 1, reagent unit, counting cell unit, sampling needle 3 and DIFF
Channel measurement unit 2, for the distribution of reagent and dilution；The whole machine that dilution allocation unit 1 is used for dilution 4 is distributed；Institute
State DIFF channel measurements unit 2 to be made up of LASER Light Source, photelectric receiver and flow chamber, complete the measurement of DIFF passages；It is described
Reagent unit includes dilution 4, WBC reagents 5, a DIFF reagents 6, the 2nd DIFF reagents 7, a CRP reagents 8 and second
CRP reagents 9；The counting cell unit includes CRP ponds 19, DIFF ponds 20, WBC ponds 21, RBC ponds 22, for completing CRP passage samples
Reaction and the RBC passage samples of this reaction with reagent, the reaction of DIFF channel samples and reagent, WBC channel samples and reagent
This reaction with reagent；The sampling needle 3 is used to complete the absorption and distribution of blood sample；The negative pressure chamber 23 is used to be WBC ponds 21
Negative pressure is provided with RBC ponds 22；The waste liquid that the discharging of waste liquid unit 24 is used to produce in whole machine is discharged outside machine.
Can functionally be divided into DIFF channel measurements unit, impedance and HGB channel measurements unit again from measurement and CRP measurements are single
Unit.
Wherein, the syringe unit is comprising the first syringe module 15, the second syringe module 16, the 3rd syringe
The syringe module 18 of module 17 and the 4th.The first syringe module 15 drags what three syringes were driven by a motor for one
Three syringe tube compositions, these three syringe tubes are respectively intended to distribution WBC reagents 5, a DIFF reagents 6, the 2nd DIFF examinations
Agent 7.The second syringe module 16 is that one-to-one syringe is to drive the syringe tube put to constitute by a motor, this
One syringe tube is allocated dilution 4 for connecting dilution allocation unit 1.The 3rd syringe module 17 is dragged for one
Two syringes are that two syringe tubes driven by a motor are constituted, and the two syringe tubes are respectively intended to connect sampling needle 3
Carry out blood sampling point blood and sample will be pushed away flow into DIFF channel measurements unit 2 to measure.The 4th syringe module 18 is one
Three injection-tubes for dragging three syringes to be driven by a motor are constituted, and these three syringe tubes are respectively intended to push away sheath stream parcel sample
This stream carries out DIFF channel measurements, the 2nd CRP reagents 9 of the first CRP reagents 8 of distribution and distribution into DIFF channel measurements unit 2.
The dilution allocation unit 1 includes pipeline, joint and valve, is connected by setting up pipeline with the second syringe module 16
Connect and coordinate the whole machine distribution for completing dilution.
The DIFF channel measurements unit 2 is made up of LASER Light Source, photelectric receiver and flow chamber, in the 3rd syringe mould
Under the collective effect of the syringe module 18 of block 17 and the 4th, sample flow is wrapped the measurement that DIFF passages are completed by detection zone.
WBC (leucocyte) pond 21, RBC (red blood cell) pond 22, negative pressure chamber 23 constitutes impedance and HGB channel measurement lists
There are HGB measuring units in unit, wherein WBC ponds 21, HGB measuring units are made up of LED, photelectric receiver, by LED by light
It is radiated at through the reacted blood sample of WBC reagents 5, HGB is then calculated according to the light intensity that optoelectronic receiver is received, wherein described
WBC reagents 5 are added in WBC ponds 21 by the first triple valve 10 from syringe.After negative pressure chamber 23 sets up negative pressure, WBC ponds 21
With the cell in RBC ponds 22 under the driving of negative pressure, negative pressure chamber 23 is flowed to, flow process each cell is counted, so as to complete
The measurement of WBC, RBC cell.
With further reference to Fig. 1 and 2, the CRP ponds 19 specifically include LASER Light Source 25, CRP examinations for CRP measuring units
Agent 8 adds mouth 26, the 2nd CRP reagents 9 to add mouth 29, dilution to add mouth 28, CRP ponds matrix 27 and photelectric receiver 30.The
One CRP reagents 8 and the 2nd CRP reagents 9 are carried out instead after the 4th triple valve 13 and the 5th triple valve 14 are added to blood sample respectively
Should, the LASER Light Source 25 and photelectric receiver 30 are arranged on the side wall of CRP ponds matrix 27, and LASER Light Source 25 launches laser
It is radiated on the solution of reaction, LASER Light Source 25 is received on photelectric receiver 30 and launches scattering light, according to dissipates for receiving
Penetrating light intensity can calculate CRP concentration.
With further reference to Fig. 3, a CRP reagents 8 and the 2nd CRP reagents 9 are stored in reagent refrigerating unit, described
Reagent refrigerating unit includes cooling fan 31, cooling piece 32, conduction cooling matrix 33 and upper lid 34.It is provided with below cooling piece 32 cold
But fan 31, conduction cooling matrix 33 is arranged on cooling piece 32, and surrounds multiple intervals, a CRP reagents 8 and the 2nd CRP
Reagent 9 is placed in the interval that cooling piece 32 and conduction cooling matrix 33 are surrounded, and lid 34 is provided with interval.Whole refrigerating system
At 2-8 DEG C, a CRP reagents 8 and the 2nd CRP reagents 9 can be preserved for a long time at such a temperature for control, and ice is put into without individually taking-up
Case is refrigerated.
As shown in figs. 1 and 4, a CRP reagents 8 and the 2nd CRP reagents 9 be need to be preheated first before,
It is heated to add CRP measuring units 19 after corresponding temperature and is reacted.First DIFF reagents 6 and the 2nd DIFF reagents 7 are same
Sample needs to be preheated, and is heated to add DIFF ponds 20 after corresponding temperature and is reacted, wherein the He of a DIFF reagents 6
Be added to reagent in DIFF ponds 20 by the second triple valve 11 and the 3rd triple valve 12 respectively by the 2nd DIFF reagents 7.This four kinds
Reagent shares a preheating unit, the preheating unit include heating plate 35, heat conduction substrate 36, the heat conducting pipe 37 of CRP reagents 8,
The heat conducting pipe 38 of CRP reagents 9, the heat conducting pipe 39 of a DIFF reagents 6 and the heat conducting pipe 40 of the 2nd DIFF reagents 7.Wherein, heat conduction substrate 36
It is metal heat-conducting material with each heat conducting pipe.The shared same preheating unit of four kinds of reagents, it is simple and reliable for structure.
The fluid dispensing system of the blood cell analyzer of this band CRP detections is allocated by syringe completely, without
Additionally increase other systems (air supply system, air-channel system, barrier film constant displacement pump) to carry out liquid distribution, simple system reliability, into
This is low.
First CRP reagents 8 and the 2nd CRP reagents 9 are allocated and are quantified by syringe, are carried out with by sampling needle
The distribution of CRP reagents is compared with quantitative scheme：A) CRP reagents can be avoided to be taken caused by the contact of residual blood sample with sampling needle
Band pollution effect；B) distribution of a CRP reagents 8, the distribution of the 2nd CRP reagents 9 can distribute parallel with blood sample, when can save
Between, test speed is fast；C) quantitative accuracy is high；D) CRP measuring systems and blood routine detecting system are separated, it is to avoid mutual detection
Influence.
Based on said apparatus, the present invention provides a kind of whole blood CRP detection methods, and specific method flow is as follows：
Step S1：By sampling needle draw blood sample；
Step S2：Blood sample in sampling needle is divided into it is appropriate in CRP ponds, while by a CRP reagents and second
CRP reagents are reacted in adding CRP ponds by the 4th syringe module, and two kinds of CRP reagents enter before adding through preheating unit
Row reagent is preheated；
Step S3：Blood sample in sampling needle is divided into it is appropriate in DIFF ponds, while by a DIFF reagents and the
Two DIFF reagents add DIFF ponds to be reacted by the first syringe module, through preheating unit before two kinds of DIFF reagents heating
Carry out reagent preheating；
Step S4：Blood sample in sampling needle is divided into it is appropriate in WBC ponds, by after diluted, sampling needle
A certain amount of diluted sample is drawn from WBC ponds, is then carried out instead by the first syringe module a certain amount of WBC reagents of addition
Should；
Step S5：Sampling needle is moved into RBC ponds, the diluted sample drawn from WBC ponds is divided into；
Step S6：CRP detections, DIFF detections, WBC/HGB detections, RBC detections carry out parallel detection measurement, are measured
After export accordingly result, and each passage is completed to clean.
Advantages of the present invention is as follows：
1) cellanalyzer of the classification of detection CRP and five blood routine while the present invention is provided, each sense channel is parallel
Detection, test speed is fast, can simultaneously export within one minute the classification blood routine results of CRP and five, and current other instruments prestissimo is
90 seconds；
2) the cellanalyzer fluid system of the classification of detection CRP and five blood routine need to only pass through while the present invention is provided
Syringe module can complete the distribution of reagent and blood sample with quantitatively, be quantified without increasing air supply system, air-channel system and barrier film
Pump, simple system reliability, low cost；
3) band CRP reagent refrigerating devices, thermostatic control can be in long-term storage instrument, without individually take out after the completion of detection
CRP reagents are put into refrigerator cold-storage, easy to operate；
4) DIFF passages reagent preheating shares a preheating unit with CRP passages reagent preheating, and simple system can
Lean on, low cost.
It should be appreciated that application of the invention is not limited to above-mentioned citing, and for those of ordinary skills, can
To be improved according to the above description or converted, all these modifications and variations should all belong to the guarantor of appended claims of the present invention
Shield scope.
Claims (9)
1. a kind of whole blood CRP detection means, it is characterised in that surveyed including syringe unit, dilution allocation unit, DIFF passages
Amount unit, reagent unit, counting cell unit, sampling needle, negative pressure chamber and discharging of waste liquid unit, the syringe unit connection dilution
Liquid allocation unit, reagent unit, counting cell unit, sampling needle and DIFF channel measurement units, for reagent and dilution point
Match somebody with somebody；The whole machine that dilution allocation unit is used for dilution is distributed；The DIFF channel measurements unit is by LASER Light Source, opto-electronic receiver
Device and flow chamber are constituted, and complete the measurement of DIFF passages；The reagent unit includes dilution, WBC reagents, DIFF examinations
Agent, the 2nd DIFF reagents, a CRP reagents and the 2nd CRP reagents；The counting cell unit includes CRP ponds, DIFF ponds, WBC
Pond, RBC ponds, reaction, the reaction of DIFF channel samples and reagent, WBC channel samples for completing CRP channel samples and reagent
The reaction of reaction and RBC channel samples and reagent with reagent；The sampling needle is used to complete the absorption and distribution of blood sample；Institute
Negative pressure chamber is stated for being that WBC ponds and RBC ponds provide negative pressure；The waste liquid that the discharging of waste liquid unit is used to produce in whole machine discharges machine
Outside device；
The syringe unit is comprising the first syringe module, the second syringe module, the 3rd syringe module and the 4th note
Emitter module, the first syringe module drags three syringes for one, and three syringe tubes driven by a motor are constituted, the
Three syringe tubes of one syringe module are respectively intended to distribute WBC reagents and DIFF reagents；The second syringe module is
One-to-one syringe, drives the syringe tube put to constitute, a syringe tube of the second syringe module by a motor
Dilution is allocated for connecting dilution allocation unit；The 3rd syringe module is one drag two syringe, by one
Two syringe tubes composition that motor drives, two syringe tubes of the 3rd syringe module are respectively intended to connection sampling needle to be carried out
Blood sampling point blood and sample will be pushed away flow into DIFF channel measurement units and measure；The 4th syringe module drags three injections for one
Device, three injection-tubes driven by a motor are constituted, and three syringe tubes of the 4th syringe module are respectively intended to push away sheath stream
Parcel sample flow carries out DIFF channel measurements and distribution CRP reagents into DIFF channel measurements unit.
2. whole blood CRP detection means according to claim 1, it is characterised in that the DIFF channel measurements unit includes
LASER Light Source, photelectric receiver and flow chamber, under the collective effect of the 3rd syringe module and the 4th syringe module, sample
Stream is wrapped the measurement that DIFF passages are completed by detection zone.
3. whole blood CRP detection means according to claim 2, it is characterised in that the WBC ponds, RBC ponds and negative pressure chamber's group
Into impedance and HGB channel measurement units, wherein there are HGB measuring units on WBC ponds, HGB measuring units are by LED and opto-electronic receiver
Device is constituted, by blood sample of the LED by light irradiation after through WBC reagent reactings, the light for then being received according to photelectric receiver
HGB is calculated by force, wherein the WBC reagents are added in WBC ponds by the first triple valve from the first syringe, negative pressure chamber builds
After vertical negative pressure, the cell in WBC ponds and RBC ponds flows to negative pressure chamber under the driving of negative pressure, and flow process each cell is counted
Number, completes the measurement of WBC, RBC cell.
4. whole blood CRP detection means according to claim 2, it is characterised in that the sampling needle is in the 3rd syringe mould
The absorption and distribution of blood sample are completed in the presence of block.
5. whole blood CRP detection means according to claim 2, it is characterised in that the CRP ponds are CRP measuring units, its
Mouth, the 2nd CRP reagents is added to add mouth, dilution to add mouth, CRP ponds matrix and photoelectricity including LASER Light Source, a CRP reagents
Receiver, a CRP reagents and the 2nd CRP reagents are carried out after the 4th triple valve and the 5th triple valve are added to blood sample respectively
Reaction, the LASER Light Source and photelectric receiver are arranged on the side wall of CRP ponds matrix, and LASER Light Source is launched laser and is radiated at
On the solution of reaction, reception LASER Light Source launches scattering light on photelectric receiver, can be counted according to the scattered light intensity for receiving
Calculate CRP concentration.
6. whole blood CRP detection means according to claim 2, it is characterised in that described also including a reagent refrigerating unit
In the reagent refrigerating unit, the reagent refrigerating unit includes cooling wind for first CRP reagents and the 2nd CRP reagent storages
Fan, cooling piece, conduction cooling matrix and upper lid, are provided with cooling fan below cooling piece, and conduction cooling matrix is arranged on cooling piece, and
Multiple intervals are surrounded, a CRP reagents and the 2nd CRP reagents are placed in the interval that cooling piece and conduction cooling matrix are surrounded,
Lid is provided with interval, whole refrigerating system control is at 2-8 DEG C.
7. whole blood CRP detection means according to claim 2, it is characterised in that also including a preheating unit, be used for
The preheating of CRP reagents and DIFF reagents, is separately added into CRP ponds and DIFF ponds 20 and is reacted after heating, a DIFF
Be added to reagent in DIFF ponds by the second triple valve and the 3rd triple valve respectively by reagent and the 2nd DIFF reagents, CRP reagents
With DIFF reagents share a preheating unit, the preheating unit include heating plate, heat conduction substrate, CRP reagents heat conducting pipe,
CRP reagents heat conducting pipe, a DIFF reagents heat conducting pipe and the 2nd DIFF reagent heat conducting pipes, wherein, heat conduction base 6 and each heat conducting pipe are
Metal heat-conducting material.
8. a kind of whole blood CRP detection methods, it is characterised in that comprise the following steps：
Step S1：By sampling needle draw blood sample；
Step S2：Blood sample in sampling needle is divided into it is appropriate in CRP ponds, while a CRP reagents and the 2nd CRP are tried
Agent is reacted in adding CRP ponds by the 4th syringe module, and two kinds of CRP reagents carry out reagent before adding through preheating unit
Preheating；
Step S3：Blood sample in sampling needle is divided into it is appropriate in DIFF ponds, while by a DIFF reagents and second
DIFF reagents add DIFF ponds to be reacted by the first syringe module, enter through preheating unit before two kinds of DIFF reagents heating
Row reagent is preheated；
Step S4：Blood sample in sampling needle is divided into appropriate in WBC ponds, by after diluted, sampling needle is from WBC
A certain amount of diluted sample is drawn in pond, then adds a certain amount of WBC reagents to be reacted by the first syringe module；
Step S5：Sampling needle is moved into RBC ponds, the diluted sample drawn from WBC ponds is divided into；
Step S6：CRP detections, DIFF detections, WBC/HGB detections, RBC detections carry out parallel detection measurement, are measured rear defeated
Go out accordingly result, and each passage is completed to clean
9. a kind of blood cell analyzer, it is characterised in that be provided with the whole blood CRP described in any one in claim 1 to 7
Detection means.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510059624.5A CN104713816B (en) | 2015-02-04 | 2015-02-04 | A kind of whole blood CRP detection means and method and a kind of blood cell analyzer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510059624.5A CN104713816B (en) | 2015-02-04 | 2015-02-04 | A kind of whole blood CRP detection means and method and a kind of blood cell analyzer |
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| DE69819996T2 (en) * | 1997-09-27 | 2004-09-02 | Horiba Ltd. | Device for counting blood cells and for immunological determination using whole blood |
| US6716633B2 (en) * | 2000-09-18 | 2004-04-06 | Sysmex Corporation | Blood cell detector, blood analyzer and blood analyzing method using the detector |
| CN103389386B (en) * | 2012-05-09 | 2014-09-24 | 深圳中科强华科技有限公司 | Portable three-differential blood cell analyzer liquid path system and method thereof |
| JP5771236B2 (en) * | 2013-05-17 | 2015-08-26 | 株式会社堀場製作所 | Blood analyzer |
| CN103336130B (en) * | 2013-06-21 | 2016-03-16 | 嘉善加斯戴克医疗器械有限公司 | A kind of whole blood immunity analytical equipment and use the blood analyser of this device |
| CN104127192B (en) * | 2013-10-16 | 2016-08-10 | 深圳市帝迈生物技术有限公司 | A kind of flow cytometer fluid system and FCM analysis method |
| CN103994962B (en) * | 2014-04-25 | 2016-08-24 | 深圳市帝迈生物技术有限公司 | A kind of flow cytometer fluid system and FCM analysis method |
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