CN104678542A - Laser source device - Google Patents
Laser source device Download PDFInfo
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- CN104678542A CN104678542A CN201310643269.7A CN201310643269A CN104678542A CN 104678542 A CN104678542 A CN 104678542A CN 201310643269 A CN201310643269 A CN 201310643269A CN 104678542 A CN104678542 A CN 104678542A
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- laser
- catoptron
- source device
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- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/06—Means for illuminating specimens
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- Optics & Photonics (AREA)
- Microscoopes, Condenser (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The invention provides a laser source device, which comprises laser devices, a reflector, an automatic regulator and a laser focusing device; the automatic regulator is located on the back face of the reflector, and is used for regulating the position and the angle of the reflector. The problem that the array output of the existing laser device is not stable is solved; while a laser source beam with high coupling efficiency, high modulation depth and high quality is obtained, the long-term stability of the laser source beam is greatly improved; the laser source device can be widely used for total internal reflection fluorescence microscopes, laser confocal fluorescence microscopes, super-resolution fluorescence microscopes and the like.
Description
Technical field
The present invention relates to a kind of LASER Light Source output unit, belong to fluorescence microscopy field.
Background technology
Optical microscope occupies an important position in field of biology always.It has run through whole biological development course, comprises the discovery of cell, the observation of organelle and cell dynamic process.In nearly ISUZU company, fluorescent microscope, because of its high resolving power and imaging selectivity, occupies leading position in optical microscope family.Fluorescent microscope develops into today, and of paramount importance technological improvement is the combination of laser technology and optical microscope, i.e. the invention of laser confocal microscope.Laser confocal scanning optical microscope greatly strengthen the axial resolution of optical microscope and improves imaging definition.Subsequently, the appearance of total internal reflectance microscope makes the resolution of Z axis bring up to 100 nanometers.More it should be noted that the microscopical invention of super-resolution fluorescence in recent years makes the development of optical microscope reach unprecedented prosperity, the various imaging pattern of the exploitation that scientist is positive is to adapt to respective imaging requirements.
Existing high-resolution Imaging-PAM all be unable to do without laser as imaging source, and requires that laser can arbitrarily switch, beamstability.Usually, the LASER Light Source of commercial apparatus is by being incorporated in optical fiber after space optical coupling, and imports in microscopes optical path.The shortcoming of this mode is that coupling efficiency is low, and the impact that light beam is subject to environment is large, easily departs from and focuses on site, need regular debugging, spends a large amount of manpower maintenances.Especially the diode laser made in China angle of divergence is large, and near field hot spot is difficult to compression, and beam quality is poor, makes application more difficult.
For solving the problems of the technologies described above, the invention provides a kind of laser light-source device, object obtains high coupling efficiency, the high modulation degree of depth and the high-quality LASER Light Source bundle of long-term stability.The present invention can be applicable to multiple fluorescent microscope imaging system, comprises laser confocal fluorescence microscope, full interior angle TIRF, super-resolution fluorescence microscope etc.
Summary of the invention
The object of the present invention is to provide a kind of laser light-source device, solve existing laser array and export unstable problem, while acquisition high coupling efficiency, high modulation degree of depth high-quality LASER Light Source bundle, significantly improve the long-time stability of light source beam, laser confocal fluorescence microscope, full interior angle TIRF, super-resolution fluorescence microscope etc. can be widely used in.
According to a scheme of the present invention, it is characterized in that, comprise laser instrument, catoptron, automatic regulator and laser aggregator; Automatic regulator is positioned at mirror back surface, the position of accommodation reflex mirror and angle; Wherein, the laser that described laser instrument sends, shines on the mirror; The automatic regulator being positioned at mirror back surface regulates position and the angle of reflective mirror, reflects the laser light to laser aggregator, pools light source beam.Automatic regulator controls reflector position and the automatically accurate of angle, is realized by computer software.
In one preferred embodiment, one or more optionally in gas laser, liquid laser, solid state laser, semiconductor laser of described laser instrument.Further preferred implementation is, described laser instrument is semiconductor laser.
In one preferred embodiment, the quantity of laser instrument is more than or equal to 2, and the optical maser wavelength wherein having at least 2 laser instruments to launch is not identical.Further preferred implementation is, the quantity of described laser instrument is more than or equal to 2, and the optical maser wavelength that each laser instrument is launched is all not identical.
Those skilled in the art according to actual conditions, can select the quantity of laser instrument, kind, wavelength and power, to obtain the best light source beam measured and need.
In one preferred embodiment, described laser aggregator comprises one group of semi-transparent semi-reflecting spectroscope.Further preferred implementation is, described semi-transparent semi-reflecting spectroscopical quantity is equal with the quantity of laser instrument.
In one preferred embodiment, the quantity of described catoptron is more than or equal to 1, is less than or equal to the quantity of laser instrument.
In one preferred embodiment, the quantity of described automatic regulator is more than or equal to 1, is less than or equal to the quantity of catoptron.
In one preferred embodiment, described laser instrument, catoptron, automatic regulator three quantity are equal.
The LASER Light Source that those skilled in the art can send according to each laser instrument, selects the catoptron of suitable quantity and the quantity of automatic regulator, is as the criterion each bundle LASER Light Source parallel projection can be entered laser aggregator.
Laser light-source device provided by the present invention can be used for fluorescent microscope, and in one embodiment, from laser aggregator light source beam out, import the light path of fluorescent microscope through catoptron, concrete steps are as follows:
The laser that laser instrument sends is radiated on the catoptron with automatic regulator, and automatic regulator is accurately located by the light path of angle to laser of accommodation reflex mirror; After the laser that catoptron reflects accumulates a branch of light source beam by laser aggregator, fluorescent microscope light path is directly entered through catoptron, spectroscope is partly returned and object lens are radiated on sample through semi-transparent, the fluorescence of electromagnetic radiation arrives charge coupling device through catoptron, be called for short CCD detecting device, carry out fluorescence imaging.
Laser light-source device provided by the present invention is used for fluorescent microscope, in another embodiment, from laser aggregator light source beam out, imports the light path of fluorescent microscope through fiber optic conduction device; Described fiber optic conduction device comprises fiber coupler and optical fiber, and concrete steps are as follows:
The laser that laser instrument sends is radiated on the catoptron with automatic regulator, and position automatic regulator is accurately located by the light path of angle to laser of accommodation reflex mirror; After the laser that catoptron reflects accumulates a branch of light source beam by laser aggregator, optical fiber is entered through fiber coupler, fluorescent microscope light path is imported by optical fiber, through lens, semi-transparently partly return spectroscope and object lens are radiated on sample, the fluorescence of electromagnetic radiation arrives on CCD detecting device through catoptron and carries out fluorescence imaging.
In described laser aggregator, semi-transparent semi-reflecting eyeglass should be coaxially arranged in parallel, can through the light of the light of long wavelength, reflects short wave; Described optical fiber adopts can adopt single-mode fiber or multimode optical fiber; The efficiency of described fiber coupler should be more than or equal to 50%.
In one preferred embodiment, concrete steps are:
The laser that the laser instrument of step one, multi-wavelength is launched is radiated on position self-regulation catoptron respectively, and self-regulation catoptron is by the movement of computer software control position and rotation; Then be pooled in same light beam by the multiple laser reflected through a series of semi-transparent semi-reflecting eyeglass; Final beam is incorporated in microscopes optical path with optical fiber or spatial light mode.
Step 2, multiple laser shine on sample through reflective mirror, and the fluorescence of electromagnetic radiation arrives on CCD detecting device through catoptron and carries out optical imagery.
The invention has the beneficial effects as follows:
1, provide multi-wavelength's laser beam steady in a long-term to export, the accurate control of laser position is carried out automatically by software, saves manpower and maintenance cost.
2, described self-regulation laser output device can be used for the multiple laser that utilizes as the fluorescent microscope of light source, comprises laser confocal fluorescence microscope, full interior angle TIRF, super-resolution fluorescence microscope etc., but is not limited only to above-mentioned microscope.
Accompanying drawing explanation
Fig. 1 is an embodiment schematic diagram of laser light-source device of the present invention.
Fig. 2 is an embodiment schematic diagram of laser light-source device of the present invention.
Fig. 3 is an embodiment schematic diagram of laser light-source device of the present invention, and A is exported light source beam, imports fluorescent microscope schematic diagram through catoptron; B is exported light source beam, imports fluorescent microscope schematic diagram through fiber optic conduction device.
Fig. 4 is the light source beam that laser light-source device of the present invention exports, and imports the index path after fluorescent microscope through catoptron.
Fig. 5 is the light source beam that laser light-source device of the present invention exports, and imports the index path after fluorescent microscope through fiber optic conduction device.
Fig. 6 is the biological sample image that laser light-source device of the present invention obtains for fluorescent microscope.
Reference numeral in accompanying drawing is as follows:
10-laser instrument; 12-catoptron; 14-automatic regulator; 16-laser aggregator; 18-catoptron; 20-fiber optic conduction device; 200-fiber coupler; 202-optical fiber.
Embodiment
Below in conjunction with drawings and Examples, the present invention is described in further detail, but the present invention is not limited to these drawings and Examples.
Fig. 1 is an embodiment schematic diagram of laser light-source device of the present invention, 5 laser instruments 10 send the laser that wavelength is 640nm, 561nm, 532nm, 473nm and 405nm respectively, be radiated on a catoptron 12, there is an automatic regulator 14 at catoptron 12 back side, by position and the angle of computer software regulating and controlling catoptron 12, the light path of laser is accurately located; After the laser aggregator 16 that the laser that catoptron 12 reflects is made up of 5 semi-transparent semi-reflecting spectroscopes accumulates a branch of light source beam, enter optical fiber 202 through fiber coupler 200, import fluorescent microscope light path by optical fiber 202.After light source beam enters fluorescent microscope, through lens, semi-transparently partly return spectroscope and object lens are radiated on sample, the fluorescence of electromagnetic radiation arrives CCD detecting device through catoptron and carries out fluorescence imaging, as shown in Figure 5.
An embodiment of laser light-source device of the present invention as shown in Figure 2,5 laser instruments 10 send the laser that wavelength is 640nm, 561nm, 532nm, 473nm and 405nm respectively, be radiated on 5 catoptrons 12 respectively, there is an automatic regulator 14 at catoptron 12 back side, by position and the angle of computer software regulating and controlling catoptron 12, the light path of each Shu Jiguang is accurately located; After the laser aggregator 16 that the laser that catoptron 12 reflects is made up of 5 semi-transparent semi-reflecting spectroscopes accumulates a branch of light source beam, directly enter fluorescent microscope light path through catoptron 18.After light source beam enters fluorescent microscope, partly return spectroscope and object lens are radiated on sample through semi-transparent, the fluorescence of electromagnetic radiation arrives CCD detecting device through catoptron and carries out fluorescence imaging, as shown in Figure 4.
An embodiment of laser light-source device of the present invention as shown in Figure 3.5 laser instruments 10 send the laser that wavelength is 640nm, 561nm, 532nm, 473nm and 405nm respectively, be radiated on 5 catoptrons 12 respectively, respectively there is an automatic regulator 14 at 5 catoptron 12 back sides, control by computer software the position and the angle that regulate 5 catoptrons 12 respectively, the light path of each Shu Jiguang is accurately located; After the laser aggregator 16 that the laser that catoptron 12 reflects is made up of 5 semi-transparent semi-reflecting spectroscopes accumulates a branch of light source beam, directly enter fluorescent microscope light path through catoptron 18, as shown in Figure 3A; After light source beam enters fluorescent microscope, partly return spectroscope and object lens are radiated on sample through semi-transparent, the fluorescence of electromagnetic radiation arrives CCD detecting device through catoptron and carries out fluorescence imaging, as shown in Figure 4.Or enter optical fiber 202 through fiber coupler 200 after the light source beam that laser aggregator 16 collects, import fluorescent microscope light path by optical fiber 202, as shown in Figure 3 B; After light source beam enters fluorescent microscope, through lens, semi-transparently partly return spectroscope and object lens are radiated on sample, the fluorescence of electromagnetic radiation arrives CCD detecting device through catoptron and carries out fluorescence imaging, as shown in Figure 5.
Concrete steps are as follows:
Step one, first, the laser that LASER Light Source sends respectively impinges upon on respective automated location regulator, and computer software needs position and the angle of shift position regulator according to the change of position.
Step 2, folded light beam impinge upon on semi-transparent semi-reflecting eyeglass, and collect in same light path.Be incorporated in microscopical light path by the mode of spatial light and coupling fiber respectively.
Step 3, light beam impinge upon on sample through catoptron reflection, and the fluorescence of electromagnetic radiation is radiated on CCD detecting device through catoptron and carries out imaging.
In above-mentioned step one, laser instrument must select fixed wave length, laser quality high, and wavelength is not limit.Automated location regulator is arranged on fixing position, is moved by its position of computer software control.
In above-mentioned step 2, semi-transparent semi-reflecting eyeglass requires coaxial, can through the light of the light of long wavelength, reflects short wave.When optical fiber exports, general employing single-mode fiber or multimode optical fiber, fiber coupler requires that efficiency reaches more than 50%.Require when spatial light exports that reflected light position is just in time irradiated on the catoptron in microscopes optical path.
In above-mentioned step 3, light beam exports through optical fiber the method expanding or directly import and enters into microscope.Light beam irradiation produces fluorescence on sample, and fluorescence impinges upon on CCD detecting device through reflection and carries out imaging.
Above-mentioned self-regulation Laser output system, laser beam can maintain a long-term stability, by the change of computer software and rectifiable light-beam position.Self-regulation Laser output system of the present invention can provide light source for multiple fluorescent microscope, comprises laser confocal fluorescence microscope, full interior angle TIRF, super-resolution fluorescence microscope etc., can be used for the field such as biology, material.
Embodiment 1
High-resolution imaging research is carried out to the structure of intracellular protein, by the living cells through fluorescence transfection, cultivates evenly adherent in the medium, be placed in the sample stage of full interior angle TIRF.Adopt laser light-source device as shown in Figure 3 B, for full interior angle TIRF provides light source beam, to intracellular protein imaging, result is exported by computer, as shown in Figure 6, clearly shows the Subfilament Structure of intracellular protein.
It should be pointed out that the professional and technical personnel for making the art, under the prerequisite not departing from the technology of the present invention principle, is what can realize the multiple amendment of above-described embodiment, and these amendments also should be considered as in the scope that the present invention should protect.
Claims (10)
1. a laser light-source device, is characterized in that, comprises laser instrument (10), catoptron (12), automatic regulator (14) and laser aggregator (16); Automatic regulator (14) is positioned at catoptron (12) back side, the position of accommodation reflex mirror (12) and angle;
The laser that described laser instrument (10) sends, impinges upon on catoptron (12); The automatic regulator (14) being positioned at catoptron (12) back side regulates position and the angle of reflective mirror (12), reflects the laser light to laser aggregator (16), pools light source beam.
2. laser light-source device according to claim 1, is characterized in that, the quantity of described laser instrument (10) is more than or equal to 2, and the optical maser wavelength wherein having at least 2 laser instruments (10) to launch is different.
3. laser light-source device according to claim 1, is characterized in that, the quantity of described laser instrument (10) is more than or equal to 2, and the optical maser wavelength that each laser instrument is launched is all not identical.
4. laser light-source device according to claim 1, is characterized in that, described laser instrument (10) one or more in gas laser, semiconductor laser or solid state laser optional.
5. laser light-source device according to claim 1, is characterized in that, described laser instrument (10) is semiconductor laser.
6. laser light-source device according to claim 1, is characterized in that, described laser aggregator (16) comprises one group of semi-transparent semi-reflecting spectroscope.
7. laser light-source device according to claim 6, is characterized in that, in described laser aggregator (16), semi-transparent semi-reflecting spectroscopical quantity is equal with the quantity of laser instrument (10).
8. the arbitrary laser light-source device according to claim 1-7, is characterized in that, the quantity of described catoptron (12) is more than or equal to 1, is less than or equal to the quantity of laser instrument (10).
9. the arbitrary laser light-source device according to claim 1-7, is characterized in that, the quantity of described automatic regulator (14) is more than or equal to 1, is less than or equal to the quantity of catoptron (12).
10. the arbitrary laser light-source device according to claim 1-7, is characterized in that, described laser instrument (10), catoptron (12), automatic regulator (14) three's quantity are equal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310643269.7A CN104678542A (en) | 2013-12-02 | 2013-12-02 | Laser source device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310643269.7A CN104678542A (en) | 2013-12-02 | 2013-12-02 | Laser source device |
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| CN104678542A true CN104678542A (en) | 2015-06-03 |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106195929A (en) * | 2016-09-03 | 2016-12-07 | 超视界激光科技（苏州）有限公司 | Laser light source module |
| CN106680984A (en) * | 2016-12-16 | 2017-05-17 | 大连光耀辉科技有限公司 | Laser binary channel simultaneous output device and microscope |
| CN106997122A (en) * | 2015-11-30 | 2017-08-01 | 株式会社日本显示器 | Light-emitting device and display device |
| CN107703616A (en) * | 2016-08-08 | 2018-02-16 | 大连光耀辉科技有限公司 | Multi-channel laser output equipment and fluorescence microscope |
| CN107703614A (en) * | 2016-08-08 | 2018-02-16 | 大连光耀辉科技有限公司 | Laser output equipment and fluorescence microscope |
| CN110006860A (en) * | 2019-03-27 | 2019-07-12 | 华南师范大学 | A kind of burnt multichannel fluorescence detecting system of copolymerization |
| CN114815132A (en) * | 2022-05-16 | 2022-07-29 | 江苏镭创高科光电科技有限公司 | Dimming system and dimming method of gradient array reflector |
-
2013
- 2013-12-02 CN CN201310643269.7A patent/CN104678542A/en active Pending
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106997122A (en) * | 2015-11-30 | 2017-08-01 | 株式会社日本显示器 | Light-emitting device and display device |
| CN107703616A (en) * | 2016-08-08 | 2018-02-16 | 大连光耀辉科技有限公司 | Multi-channel laser output equipment and fluorescence microscope |
| CN107703614A (en) * | 2016-08-08 | 2018-02-16 | 大连光耀辉科技有限公司 | Laser output equipment and fluorescence microscope |
| CN106195929A (en) * | 2016-09-03 | 2016-12-07 | 超视界激光科技（苏州）有限公司 | Laser light source module |
| CN106680984A (en) * | 2016-12-16 | 2017-05-17 | 大连光耀辉科技有限公司 | Laser binary channel simultaneous output device and microscope |
| CN106680984B (en) * | 2016-12-16 | 2019-06-11 | 大连光耀辉科技有限公司 | A kind of laser binary channels output equipment and microscope simultaneously |
| CN110006860A (en) * | 2019-03-27 | 2019-07-12 | 华南师范大学 | A kind of burnt multichannel fluorescence detecting system of copolymerization |
| CN114815132A (en) * | 2022-05-16 | 2022-07-29 | 江苏镭创高科光电科技有限公司 | Dimming system and dimming method of gradient array reflector |
| CN114815132B (en) * | 2022-05-16 | 2023-12-01 | 江苏镭创高科光电科技有限公司 | Dimming system and dimming method for gradient array reflector |
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