CN104673890B - Cotton black aphid PCR quick determination methods based on specific SS COI primers - Google Patents
Cotton black aphid PCR quick determination methods based on specific SS COI primers Download PDFInfo
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- CN104673890B CN104673890B CN201410697874.7A CN201410697874A CN104673890B CN 104673890 B CN104673890 B CN 104673890B CN 201410697874 A CN201410697874 A CN 201410697874A CN 104673890 B CN104673890 B CN 104673890B
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- Prior art keywords
- cotton black
- black aphid
- primer
- aphid
- cotton
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- 229920000742 Cotton Polymers 0.000 title claims abstract description 50
- 241001151957 Aphis aurantii Species 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 12
- 238000001514 detection method Methods 0.000 claims description 10
- 238000006243 chemical reaction Methods 0.000 claims description 8
- 238000012408 PCR amplification Methods 0.000 claims description 7
- 238000000246 agarose gel electrophoresis Methods 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 2
- 108020004414 DNA Proteins 0.000 abstract description 20
- 230000003321 amplification Effects 0.000 abstract description 6
- 238000003199 nucleic acid amplification method Methods 0.000 abstract description 6
- 108020005196 Mitochondrial DNA Proteins 0.000 abstract description 3
- 230000000694 effects Effects 0.000 abstract description 3
- 235000013305 food Nutrition 0.000 abstract description 3
- 238000004458 analytical method Methods 0.000 abstract 1
- 241000219146 Gossypium Species 0.000 description 40
- 241001124076 Aphididae Species 0.000 description 6
- 241000238631 Hexapoda Species 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 4
- 108090000623 proteins and genes Proteins 0.000 description 4
- 238000013461 design Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000002965 ELISA Methods 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 239000013642 negative control Substances 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 241001600407 Aphis <genus> Species 0.000 description 1
- 241000238421 Arthropoda Species 0.000 description 1
- 101150087323 COI gene Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 240000002024 Gossypium herbaceum Species 0.000 description 1
- 235000004341 Gossypium herbaceum Nutrition 0.000 description 1
- 241000258937 Hemiptera Species 0.000 description 1
- 241001145009 Sophora alopecuroides Species 0.000 description 1
- 241000219793 Trifolium Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 238000002306 biochemical method Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000012154 double-distilled water Substances 0.000 description 1
- ZMMJGEGLRURXTF-UHFFFAOYSA-N ethidium bromide Chemical compound [Br-].C12=CC(N)=CC=C2C2=CC=C(N)C=C2[N+](CC)=C1C1=CC=CC=C1 ZMMJGEGLRURXTF-UHFFFAOYSA-N 0.000 description 1
- 229960005542 ethidium bromide Drugs 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
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- 230000002068 genetic effect Effects 0.000 description 1
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- 210000003716 mesoderm Anatomy 0.000 description 1
- 230000002438 mitochondrial effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 244000062645 predators Species 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
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- 102000004169 proteins and genes Human genes 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (2)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201410697874.7A CN104673890B (en) | 2014-11-19 | 2014-11-19 | Cotton black aphid PCR quick determination methods based on specific SS COI primers |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN201410697874.7A CN104673890B (en) | 2014-11-19 | 2014-11-19 | Cotton black aphid PCR quick determination methods based on specific SS COI primers |
Publications (2)
Publication Number | Publication Date |
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CN104673890A CN104673890A (en) | 2015-06-03 |
CN104673890B true CN104673890B (en) | 2018-02-02 |
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CN201410697874.7A Expired - Fee Related CN104673890B (en) | 2014-11-19 | 2014-11-19 | Cotton black aphid PCR quick determination methods based on specific SS COI primers |
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CN (1) | CN104673890B (en) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106609306A (en) * | 2016-06-17 | 2017-05-03 | 新疆农业科学院植物保护研究所 | Rapid chrysopa formosa brauer PCR detection based on specific SS-COI primers |
CN110656186B (en) * | 2019-10-31 | 2023-07-14 | 新疆农业科学院植物保护研究所 | Kit and method for rapidly identifying walnut black spot aphids |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103436618A (en) * | 2013-08-23 | 2013-12-11 | 中国农业科学院植物保护研究所 | Aphis gossypii Glover specificity SS-COI primers, kit containing primer and detection method of kit |
-
2014
- 2014-11-19 CN CN201410697874.7A patent/CN104673890B/en not_active Expired - Fee Related
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103436618A (en) * | 2013-08-23 | 2013-12-11 | 中国农业科学院植物保护研究所 | Aphis gossypii Glover specificity SS-COI primers, kit containing primer and detection method of kit |
Non-Patent Citations (2)
Title |
---|
"DNA Barcoding and the Associated PhylAphidB@se Website for the Identification of European Aphids (Insecta Hemiptera Aphididae)";Armelle Coeur dˊacier et al.;《PLOS ONE》;20160604;第9卷(第6期);摘要和第2页右栏 DNA Extraction, Amplification and Sequencing * |
"Use of a mitochondrial COI sequence to identify species of the subtribe Aphidina (Hemiptera, Aphididae)";Jian Feng Zhang et al.;《ZooKeys》;20110811;第1-17页 * |
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CN104673890A (en) | 2015-06-03 |
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EXSB | Decision made by sipo to initiate substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
CB03 | Change of inventor or designer information | ||
CB03 | Change of inventor or designer information |
Inventor after: Wang Dongmei Inventor after: Li Haobin Inventor after: Li Xiaorong Inventor after: Ding Ruifeng Inventor after: Liu Jian Inventor after: Liu Xinlan Inventor after: Li Haiqiang Inventor after: Li Guangkuo Inventor after: Fang Shijie Inventor after: Xu Yao Inventor after: Akedan Inventor before: Wang Dongmei Inventor before: Ding Ruifeng Inventor before: Liu Jian Inventor before: Li Haiqiang Inventor before: Li Haobin Inventor before: Xu Yao Inventor before: Akedan Inventor before: Wang Fei Inventor before: Li Guangkuo |
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CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180202 |