CN104673704B - The pseudomonas aeruginosa strains of one plant of degraded agricultural chemicals containing ester bond - Google Patents
The pseudomonas aeruginosa strains of one plant of degraded agricultural chemicals containing ester bond Download PDFInfo
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- CN104673704B CN104673704B CN201410774238.XA CN201410774238A CN104673704B CN 104673704 B CN104673704 B CN 104673704B CN 201410774238 A CN201410774238 A CN 201410774238A CN 104673704 B CN104673704 B CN 104673704B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/38—Pseudomonas
- C12R2001/385—Pseudomonas aeruginosa
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/306—Pesticides
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Abstract
The invention belongs to application environment microorganism and agriculture field, specifically disclose one plant of degraded agricultural chemicals containing ester bond pseudomonas aeruginosa (Pseudomonas aeruginosa) bacterial strain PAO1, culture presevation number is CCTCC M 2014630.The strains for degrading spectrum is wide, the agricultural chemicals containing ester bond such as degradable effective cypermethrin, gamma cyhalothrin, decis, cypermethrin, Fenpropathrin, Biphenthrin, kresoxim-methyl, pyraclostrobin and Fluoxastrobin.The bacterial strain environmental suitability is strong, and good degrading effect is easy to use, available for agricultural chemicals of the degraded containing ester bond, has very important application value in terms of the residues of pesticides removal in the environment such as water body, soil.
Description
Technical field
The present invention relates to application environment microorganism and agriculture field.More particularly, to one plant of agricultural chemicals containing ester bond of degrading
Pseudomonas aeruginosa strains.
Background technology
A large amount of, frequent use of agricultural chemicals brings serious harm, particularly pyrethroid to ecological environment and human health
The pesticide residual contamination problem brought that widely uses of ester pesticide and methoxy acrylic bactericide can not be ignored.This
Two class agricultural chemicals are the agricultural chemicals containing ester bond in structure, are the main Types on current pesticide market.Numerous studies show that this two
Pesticide residue not only causes great harm to non-target organism such as honeybee, fish and aquatic invertebrate etc., and right
The mankind and other mammals have a chronic toxicity, Long Term Contact or take in this two classes agricultural chemicals and will cause chronic disease, or even have
Carcinogenic, teratogenesis, mutagenic danger.Therefore, the pyrethroid and methoxy acrylic agricultural chemicals eliminated in environment is residual
Pollution is stayed to turn into the researcher scientific research proposition with great economy and social effect urgently to be resolved hurrily.
In recent years, as the improvement of people's living standards, pollution-free food that people are polluted in the urgent need to non agricultural chemical residuum and
Cleaning ambient.And can not also be reduced in agricultural production process Pesticides.Therefore, seek at efficient, safe, economic residues of pesticides
New technology is managed, the contradiction of prevention and control of plant diseases, pest control medication and residues of pesticides is solved, it appears particularly necessary.Biodegradation is that residues of pesticides are controlled
Frontier, the new way of reason, have easy to operate, economical and practical, environment-friendly etc. excellent compared with traditional physico-chemical process
Point, gathers around and has broad application prospects.The precedent of oil pollution is successfully administered using microorganism in the world.Domestic Beijing is good
Successfully production " than sub- " catabolic enzyme preparation is used to administer organophosphorus insecticide pollution for Nong Xin Trade Developments Co., Ltd.But,
There is presently no the microorganism specifically designed for the agricultural chemicals containing ester bond and its biodegradable formulations product.
The content of the invention
It is an object of the invention to the above-mentioned deficiency for prior art, there is provided the verdigris of one plant of degraded agricultural chemicals containing ester bond is false
Aeromonas strain.The bacterial strain can effectively degrade effective cypermethrin, gamma cyhalothrin, decis, cypermethrin, first cyanogen
The agricultural chemicals containing ester bond such as chrysanthemum ester, Biphenthrin, kresoxim-methyl, pyrazoles ethofenprox and Fluoxastrobin, therefore, the bacterial strain is in water body, soil
There is very important application value in terms of residues of pesticides removal in the environment such as earth.
Another object of the present invention is to provide application of the above-mentioned bacterial strains in the preparation for preparing degraded agricultural chemicals containing ester bond.
Another object of the present invention is to provide a kind of preparation for the agricultural chemicals containing ester bond of degrading.
Another object of the present invention is to provide application of the above-mentioned bacterial strains in degraded agricultural chemicals containing ester bond.
Above-mentioned purpose of the present invention is achieved through the following technical solutions：
One plant degraded the agricultural chemicals containing ester bond pseudomonas aeruginosa (Pseudomonas aeruginosa) bacterial strain PAO1, institute
State bacterial strain and be preserved in China typical culture collection center on December 5th, 2014（CCTCC）, preserving number is CCTCC NO: M
2014630.The address of the China typical culture collection center is Wuhan City, Hubei Province Wuhan University.
The pseudomonas aeruginosa (Pseudomonas aeruginosa) bacterial strain PAO1 by enrichment culture method to wild
Type bacterial strain carries out repeated screening rejuvenation, makes it have height resistance, the ability of degraded stabilization.
The pseudomonas aeruginosa (Pseudomonas aeruginosa) bacterial strain PAO1 grown in LB solid plates compared with
It hurry up, colonial morphology is that yellow green, center projections, edge are flat.Determined by physio-biochemical characteristics, show that the bacterial strain belongs to leather blue
Family name's negative bacillus, aerobic, oxidizing ferment, gelatin liquefaction, nitrate reduction reacting positive；Indole reaction is negative.Bacterium decomposable asymmetric choice net profit
Use glucose and xylose；Do not decompose and utilize sucrose and lactose.
It is of the invention to be found by studying, pseudomonas aeruginosa (Pseudomonas aeruginosa) bacterial strain PAO1 can be effective
Degraded effective cypermethrin, gamma cyhalothrin, decis, cypermethrin, Fenpropathrin, Biphenthrin, kresoxim-methyl, pyrrole
The agricultural chemicals containing ester bond such as azoles kresoxim-methyl and Fluoxastrobin, therefore claimed bacterial strain PAO1 is preparing agriculture containing ester bond of degrading
Application in the preparation of medicine.
A kind of preparation for the agricultural chemicals containing ester bond of degrading, containing pseudomonas aeruginosa strains PAO1 as described above and other assistant agents.
The application of bacterial strain agricultural chemicals containing ester bond in degraded water body or soil.
Compared with prior art, the invention has the advantages that：
1. the invention provides a Pseudomonas aeruginosa strain (Pseudomonas aeruginosa) bacterial strain PAO1, through experiment
Prove, the bacterial strain can effectively degrade in a short time effective cypermethrin, gamma cyhalothrin, decis, cypermethrin,
The agricultural chemicals containing ester bond such as Fenpropathrin, Biphenthrin, kresoxim-methyl, pyraclostrobin and Fluoxastrobin, degradation efficiency is high.
2. the pseudomonas aeruginosa strains PAO1 environmental suitabilities that the present invention is provided are strong, easy to use, therefore, the bacterial strain exists
There is very important application value in terms of repairing the environment such as water body, the soil of pesticide residual contamination.
Brief description of the drawings
Fig. 1 bacterial strains PAO1 degraded effective cypermethrins produce obvious transparent circle.
Fig. 2 bacterial strains PAO1 degrades over time effective cypermethrin (100 μ g/mL) HPLC chromatogram.
The specific degradation rate of Fig. 3 bacterial strains PAO1 degraded various concentrations effective cypermethrins.
Embodiment
The present invention is further elaborated on reference to Figure of description and specific embodiment.Following examples are this hair
Bright preferably embodiment, but protection scope of the present invention is not limited in any form.Main elaboration of the invention is described
The replacement of simple parameter can not go to live in the household of one's in-laws on getting married in embodiment one by one in bacterial strain and the application thought based on the bacterial strain, embodiment
State, but and be not so limited the change made under the present invention, other any Spirit Essences and principle without departing from the present invention, repair
Adorn, substitute, combine, simplify, equivalent substitute mode should be considered as, all should be comprising within the scope of the present invention.
Unless stated otherwise, the present invention is used reagent, method and apparatus for the art conventional reagent, method and are set
It is standby.Unless stated otherwise, agents useful for same and material of the present invention are purchased in market.
The bacterial strain PAO1 of embodiment 1 enrichment culture
The present inventor carries out repeated screening rejuvenation by enrichment culture method to wild-type strain PAO1, makes with highly resistance to
The stable ability of medicine, degraded.For culture medium based on the culture medium of enrichment culture（It is formulated and is：(NH4)2SO4, 2 g;
MgSO4, 0.2 g; CaCl2, 0.01 g; FeSO4, 0.005 g; MnCl2, 0.002 g; K2HPO4, 10.5 g;
KH2PO4, 4.5 g, distilled water 1000 mL, pH 7.5）.Effective cypermethrin active compound mother liquor (DMSO is solvent) is added simultaneously,
It is 50 μ g/mL to make effective cypermethrin initial mass concentration, in carrying out enrichment culture under 30 DEG C, 150 r/min shaking tables.Culture
After 7 d, it is transferred to by 10% inoculum concentration in basal medium of the second batch containing 100 μ g/mL effective cypermethrins.The same terms
Cultivate after 7 d, then be transferred to by 10% inoculum concentration containing effective cypermethrin as in 200 μ g/mL basal medium, continuation is trained
Support 7 d.By that analogy, the selection pressure of agricultural chemicals, repeated screening are continuously increased.Finally take 0.2 mL basal medium zymotic fluids equal
Line separation is repeated in even be applied in the solid plate containing effective cypermethrin, until single bacterium colony is obtained, and using efficient
Liquid chromatography（HPLC）Verify its degradation effect.
The bacterial strain is preserved in China typical culture collection center（CCTCC）, preservation date is December 5 in 2014
Day, preserving number is CCTCC M 2014630, the Strain Designation be pseudomonas aeruginosa (Pseudomonas aeruginosa)
Bacterial strain PAO1.
The address of the China typical culture collection center is Wuhan City, Hubei Province Wuhan University.
The bacterial strain PAO1 of embodiment 2 physio-biochemical characteristics
The pseudomonas aeruginosa (Pseudomonas aeruginosa) bacterial strain PAO1 grown in LB solid plates compared with
It hurry up, colonial morphology is that yellow green, center projections, edge are flat.Determined by physio-biochemical characteristics, show that the bacterial strain belongs to leather blue
Family name's negative bacillus, aerobic, oxidizing ferment, gelatin liquefaction, nitrate reduction reacting positive；Indole reaction is negative.Bacterium decomposable asymmetric choice net profit
Use glucose and xylose；Do not decompose and utilize sucrose and lactose.
The bacterial strain PAO1 degrading pesticides of embodiment 3 are tested
In basal medium（Be the same as Example 1）In be separately added into effective cypermethrin, gamma cyhalothrin, bromine cyanogen chrysanthemum
Ester, cypermethrin, Fenpropathrin, Biphenthrin, kresoxim-methyl, pyraclostrobin and Fluoxastrobin, make its final concentration of 100 μ g/
mL；Inoculum concentration be 0.2 g/L, and set do not connect the culture medium of bacterium for control, 30 DEG C culture 0~5 d, periodically sample, using HPLC
Detect the residual quantity of agricultural chemicals and calculate degradation rate, the results are shown in Table shown in 1.As a result show, bacterial strain PAO1 in a short time can be effective
Degraded effective cypermethrin, gamma cyhalothrin, decis, cypermethrin, Fenpropathrin, Biphenthrin, kresoxim-methyl, pyrrole
Azoles kresoxim-methyl and Fluoxastrobin, respectively reach 91.4% in 5 d to the degradation rate of above-mentioned agricultural chemicals, 86.1%, 84.5%, 82.8%,
70.2%th, 52.2%, 87.7%, 86.3% and 68.0%, with preferable biodegradable effect.
Claims (5)
1. one plant degraded the agricultural chemicals containing ester bond pseudomonas aeruginosa (Pseudomonas aeruginosa) bacterial strain PAO1, it is special
Levy and be, the bacterial strain was preserved in China typical culture collection center on December 5th, 2014（CCTCC）, preserving number is
CCTCC M 2014630；The agricultural chemicals containing ester bond is effective cypermethrin, gamma cyhalothrin, decis, chlorine cyanogen chrysanthemum
Ester, Fenpropathrin, Biphenthrin, kresoxim-methyl, pyrazoles ethofenprox and Fluoxastrobin.
2. application of the bacterial strain described in claim 1 in the preparation for preparing degraded agricultural chemicals containing ester bond, the agricultural chemicals containing ester bond is height
Imitate cypermethrin, gamma cyhalothrin, decis, cypermethrin, Fenpropathrin, Biphenthrin, kresoxim-methyl, pyrazoles ether chrysanthemum
Ester and Fluoxastrobin.
3. a kind of preparation for the agricultural chemicals containing ester bond of degrading, it is characterised in that contain the pseudomonas aeruginosa strains described in claim 1
PAO1 and other assistant agents, the agricultural chemicals containing ester bond be effective cypermethrin, gamma cyhalothrin, decis, cypermethrin,
Fenpropathrin, Biphenthrin, kresoxim-methyl, pyrazoles ethofenprox and Fluoxastrobin.
4. bacterial strain described in claim 1 degraded agricultural chemicals containing ester bond in application, the agricultural chemicals containing ester bond be effective cypermethrin,
Gamma cyhalothrin, decis, cypermethrin, Fenpropathrin, Biphenthrin, kresoxim-methyl, pyrazoles ethofenprox and Fluoxastrobin.
5. application according to claim 4, it is characterised in that bacterial strain described in claim 1 is in degraded water body or soil
The application of the agricultural chemicals containing ester bond.
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| CN105754908B (en) * | 2016-05-03 | 2019-02-22 | 普洱学院 | Pseudomonas aeruginosa strain and application thereof |
| CN108969956B (en) * | 2018-07-27 | 2020-12-29 | 华南农业大学 | Degrading strain of bactericide kresoxim-methyl and application thereof |
| CN114045246A (en) * | 2021-12-15 | 2022-02-15 | 黄河三角洲京博化工研究院有限公司 | Pseudomonas aeruginosa for degrading chlorobenzene compounds and/or dioxin and application thereof |
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| BR112013017809A8 (en) * | 2011-01-12 | 2019-08-27 | Inocucor Tech Inc | composition, site bioremediation method, methods for affecting plant growth, for treating a surface and fluid, for preparing an article, a food supplement, and a mixed culture, and for food supplementation, and, article |
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